MX344371B - Amplificacion de señal. - Google Patents
Amplificacion de señal.Info
- Publication number
- MX344371B MX344371B MX2013005569A MX2013005569A MX344371B MX 344371 B MX344371 B MX 344371B MX 2013005569 A MX2013005569 A MX 2013005569A MX 2013005569 A MX2013005569 A MX 2013005569A MX 344371 B MX344371 B MX 344371B
- Authority
- MX
- Mexico
- Prior art keywords
- signal amplification
- compositions
- nucleic acid
- methods
- relates
- Prior art date
Links
- 230000003321 amplification Effects 0.000 title 1
- 238000003199 nucleic acid amplification method Methods 0.000 title 1
- 102000004190 Enzymes Human genes 0.000 abstract 3
- 108090000790 Enzymes Proteins 0.000 abstract 2
- 238000000034 method Methods 0.000 abstract 2
- 239000000203 mixture Substances 0.000 abstract 2
- 150000007523 nucleic acids Chemical class 0.000 abstract 2
- 238000001514 detection method Methods 0.000 abstract 1
- 102000039446 nucleic acids Human genes 0.000 abstract 1
- 108020004707 nucleic acids Proteins 0.000 abstract 1
- 239000000758 substrate Substances 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
- C12Q1/682—Signal amplification
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Immunology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
Abstract
La presente invención se refiere a un método para detectar un objetivo, caracterizado porque comprende: (a) genera run primer oligonucleótido de Fragmento Conductor (DF) a partir de un evento de reconocimiento de objetivo mediante: (1) actividad catalítica de una enzima de ácido nucleico de componentes múltiples (MNAzima); o (2) escisión de nucleasa dependiente del objetivo de un duplicado formado por hibridación entre un Oligonucleótido Iniciador Sintético (SIO) y el objetivo; (b) proporcionar un primer oligonucleótido del Sustrato Amplificador de Enzima (EAS1) y un segundo oligonucleótido del sustrato Amplificador de Enzima (EAS2) en donde una porción del EAS1 es complementaria a una porción del EAS2, y en donde una porción del EAS1 es complementaria a una porción del primer DF y; (c) poner en contacto el EAS1 y el EAS2 con el primer DF bajo condiciones que permiten; (1) ensamblar el primer DF con el EAS1 y el EAS2 para formar un primer complejo de Amplificador de Señal de Enzima Completa (CESA), y (2) forma run sitio de reconocimiento y un sitio de escisión para una primera nucleasa; en donde el enlace del primer DF a dicho EAS1 completa un sitio de reconocimiento nucleasa parcial y/o un sitio de escisión de nucleasa parcia; (d) proporcionar la primer nucleasa; y ( e) poner en contacto la primera nucleasa con el primer complejo CESA bajo condiciones que permiten la interacción de la nucleasa con el sitio de reconocimiento y escisión en el sitio de escisión, en donde la escisión por dicha primera nucleasa produce un efecto detectable indicativo de la presencia del objetivo.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| AU2010905152A AU2010905152A0 (en) | 2010-11-19 | Signal amplification | |
| PCT/AU2011/001504 WO2012065231A1 (en) | 2010-11-19 | 2011-11-21 | Signal amplification |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| MX2013005569A MX2013005569A (es) | 2014-02-03 |
| MX344371B true MX344371B (es) | 2016-12-14 |
Family
ID=46083431
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| MX2013005569A MX344371B (es) | 2010-11-19 | 2011-11-21 | Amplificacion de señal. |
Country Status (13)
| Country | Link |
|---|---|
| US (1) | US9862990B2 (es) |
| EP (1) | EP2640852B1 (es) |
| JP (1) | JP6092114B2 (es) |
| KR (1) | KR101968352B1 (es) |
| AU (1) | AU2011331920B2 (es) |
| BR (1) | BR112013012450B1 (es) |
| CA (1) | CA2818478C (es) |
| IL (1) | IL226389A (es) |
| MX (1) | MX344371B (es) |
| NZ (1) | NZ610584A (es) |
| SG (2) | SG10201509557XA (es) |
| WO (1) | WO2012065231A1 (es) |
| ZA (1) | ZA201303552B (es) |
Families Citing this family (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| SG10201509557XA (en) | 2010-11-19 | 2015-12-30 | Speedx Pty Ltd | Signal amplification |
| AU2013202354A1 (en) * | 2012-06-18 | 2014-01-16 | Speedx Pty Ltd | Target detection and signal amplification |
| CN104764790B (zh) * | 2015-03-26 | 2017-06-09 | 济南大学 | 基于核酸适配体检测链霉素的生物传感器及其制备方法 |
| EP3325666B1 (en) | 2015-07-17 | 2020-04-29 | Luminex Corporation | Methods for catalytic assays |
| CN105567839B (zh) * | 2016-02-03 | 2019-02-22 | 青岛大学 | 基于网络型核酸纳米探针的酶循环放大检测dna的比色法 |
| US10465233B2 (en) | 2016-03-25 | 2019-11-05 | The Government Of The United States Of America, As Represented By The Secretary Of The Navy | Time-resolved nucleic acid hybridization beacons |
| KR20180072480A (ko) * | 2016-12-21 | 2018-06-29 | 한국생명공학연구원 | 자가 형광 신호 증폭이 가능한 마이크로 rna 검출용 바이오 프로브 세트 및 이의 용도 |
| US20200299754A1 (en) | 2017-04-11 | 2020-09-24 | Speedx Pty Ltd | Detection cascades |
| CN108728430B (zh) * | 2017-04-21 | 2022-04-05 | 胤安国际（辽宁）基因科技股份有限公司 | 一种制备含有多个重复单元dna长探针的方法 |
| CN107884565B (zh) * | 2017-10-13 | 2019-12-24 | 广东省生态环境技术研究所 | 一种砷离子的检测方法及检测试剂盒 |
| CN109975561B (zh) * | 2019-04-25 | 2021-11-09 | 河北医科大学 | 一种基于核酸适配体的超灵敏检测多巴胺的方法 |
| CN115637287A (zh) * | 2022-06-14 | 2023-01-24 | 青岛科技大学 | 用于检测单碱基基因突变的MNAzyme生物传感器 |
Family Cites Families (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5424413A (en) * | 1992-01-22 | 1995-06-13 | Gen-Probe Incorporated | Branched nucleic acid probes |
| US20080220425A1 (en) * | 1996-01-24 | 2008-09-11 | Third Wave Technologies, Inc. | Methods and Compositions for Detecting Target Sequences |
| CN103789311A (zh) * | 2005-10-07 | 2014-05-14 | 强生研究有限公司 | 多组分核酸酶及其使用方法 |
| EP2066817B1 (en) | 2006-10-06 | 2014-07-30 | SpeeDx Pty Ltd | Molecular switches and methods for their use |
| JP2010535528A (ja) * | 2007-08-13 | 2010-11-25 | ユニバーシティ オブ ストラスクライド | 核酸配列の同定 |
| US20110207132A1 (en) * | 2008-08-04 | 2011-08-25 | University Of Maryland | Probes and methods for detecting analytes |
| WO2010019414A2 (en) * | 2008-08-15 | 2010-02-18 | Cascade Biosystems, Inc. | Detecting nucleic acid |
| SG10201509557XA (en) | 2010-11-19 | 2015-12-30 | Speedx Pty Ltd | Signal amplification |
-
2011
- 2011-11-21 SG SG10201509557XA patent/SG10201509557XA/en unknown
- 2011-11-21 MX MX2013005569A patent/MX344371B/es active IP Right Grant
- 2011-11-21 BR BR112013012450-4A patent/BR112013012450B1/pt not_active IP Right Cessation
- 2011-11-21 US US13/885,554 patent/US9862990B2/en not_active Expired - Fee Related
- 2011-11-21 WO PCT/AU2011/001504 patent/WO2012065231A1/en not_active Ceased
- 2011-11-21 JP JP2013539098A patent/JP6092114B2/ja not_active Expired - Fee Related
- 2011-11-21 NZ NZ610584A patent/NZ610584A/en not_active IP Right Cessation
- 2011-11-21 EP EP11840792.3A patent/EP2640852B1/en active Active
- 2011-11-21 AU AU2011331920A patent/AU2011331920B2/en not_active Ceased
- 2011-11-21 SG SG2013038831A patent/SG190841A1/en unknown
- 2011-11-21 CA CA2818478A patent/CA2818478C/en active Active
- 2011-11-21 KR KR1020137015622A patent/KR101968352B1/ko not_active Expired - Fee Related
-
2013
- 2013-05-16 IL IL226389A patent/IL226389A/en active IP Right Grant
- 2013-05-16 ZA ZA2013/03552A patent/ZA201303552B/en unknown
Also Published As
| Publication number | Publication date |
|---|---|
| CA2818478A1 (en) | 2012-05-24 |
| US9862990B2 (en) | 2018-01-09 |
| EP2640852A1 (en) | 2013-09-25 |
| NZ610584A (en) | 2015-02-27 |
| AU2011331920A1 (en) | 2013-05-02 |
| WO2012065231A1 (en) | 2012-05-24 |
| KR20140026343A (ko) | 2014-03-05 |
| EP2640852A4 (en) | 2014-05-14 |
| BR112013012450B1 (pt) | 2020-10-13 |
| IL226389A (en) | 2016-03-31 |
| AU2011331920B2 (en) | 2014-10-30 |
| SG10201509557XA (en) | 2015-12-30 |
| JP2013542738A (ja) | 2013-11-28 |
| BR112013012450A2 (pt) | 2016-08-30 |
| CA2818478C (en) | 2020-07-21 |
| KR101968352B1 (ko) | 2019-04-11 |
| JP6092114B2 (ja) | 2017-03-08 |
| SG190841A1 (en) | 2013-07-31 |
| MX2013005569A (es) | 2014-02-03 |
| US20140017669A1 (en) | 2014-01-16 |
| IL226389A0 (en) | 2013-07-31 |
| ZA201303552B (en) | 2014-01-29 |
| EP2640852B1 (en) | 2018-08-22 |
| CN103328655A (zh) | 2013-09-25 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FG | Grant or registration |