MX344371B - Amplificacion de señal. - Google Patents

Amplificacion de señal.

Info

Publication number
MX344371B
MX344371B MX2013005569A MX2013005569A MX344371B MX 344371 B MX344371 B MX 344371B MX 2013005569 A MX2013005569 A MX 2013005569A MX 2013005569 A MX2013005569 A MX 2013005569A MX 344371 B MX344371 B MX 344371B
Authority
MX
Mexico
Prior art keywords
signal amplification
compositions
nucleic acid
methods
relates
Prior art date
Application number
MX2013005569A
Other languages
English (en)
Other versions
MX2013005569A (es
Inventor
Velyian Todd Alison
Mokany Elisa
Meiria Linardy Evelyn
Lonergan Dina
Original Assignee
Speedx Pty Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from AU2010905152A external-priority patent/AU2010905152A0/en
Application filed by Speedx Pty Ltd filed Critical Speedx Pty Ltd
Publication of MX2013005569A publication Critical patent/MX2013005569A/es
Publication of MX344371B publication Critical patent/MX344371B/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/682—Signal amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase

Abstract

La presente invención se refiere a un método para detectar un objetivo, caracterizado porque comprende: (a) genera run primer oligonucleótido de Fragmento Conductor (DF) a partir de un evento de reconocimiento de objetivo mediante: (1) actividad catalítica de una enzima de ácido nucleico de componentes múltiples (MNAzima); o (2) escisión de nucleasa dependiente del objetivo de un duplicado formado por hibridación entre un Oligonucleótido Iniciador Sintético (SIO) y el objetivo; (b) proporcionar un primer oligonucleótido del Sustrato Amplificador de Enzima (EAS1) y un segundo oligonucleótido del sustrato Amplificador de Enzima (EAS2) en donde una porción del EAS1 es complementaria a una porción del EAS2, y en donde una porción del EAS1 es complementaria a una porción del primer DF y; (c) poner en contacto el EAS1 y el EAS2 con el primer DF bajo condiciones que permiten; (1) ensamblar el primer DF con el EAS1 y el EAS2 para formar un primer complejo de Amplificador de Señal de Enzima Completa (CESA), y (2) forma run sitio de reconocimiento y un sitio de escisión para una primera nucleasa; en donde el enlace del primer DF a dicho EAS1 completa un sitio de reconocimiento nucleasa parcial y/o un sitio de escisión de nucleasa parcia; (d) proporcionar la primer nucleasa; y ( e) poner en contacto la primera nucleasa con el primer complejo CESA bajo condiciones que permiten la interacción de la nucleasa con el sitio de reconocimiento y escisión en el sitio de escisión, en donde la escisión por dicha primera nucleasa produce un efecto detectable indicativo de la presencia del objetivo.
MX2013005569A 2010-11-19 2011-11-21 Amplificacion de señal. MX344371B (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
AU2010905152A AU2010905152A0 (en) 2010-11-19 Signal amplification
PCT/AU2011/001504 WO2012065231A1 (en) 2010-11-19 2011-11-21 Signal amplification

Publications (2)

Publication Number Publication Date
MX2013005569A MX2013005569A (es) 2014-02-03
MX344371B true MX344371B (es) 2016-12-14

Family

ID=46083431

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2013005569A MX344371B (es) 2010-11-19 2011-11-21 Amplificacion de señal.

Country Status (13)

Country Link
US (1) US9862990B2 (es)
EP (1) EP2640852B1 (es)
JP (1) JP6092114B2 (es)
KR (1) KR101968352B1 (es)
AU (1) AU2011331920B2 (es)
BR (1) BR112013012450B1 (es)
CA (1) CA2818478C (es)
IL (1) IL226389A (es)
MX (1) MX344371B (es)
NZ (1) NZ610584A (es)
SG (2) SG190841A1 (es)
WO (1) WO2012065231A1 (es)
ZA (1) ZA201303552B (es)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2012065231A1 (en) 2010-11-19 2012-05-24 Speedx Pty Ltd Signal amplification
AU2013202354A1 (en) * 2012-06-18 2014-01-16 Speedx Pty Ltd Target detection and signal amplification
CN104764790B (zh) * 2015-03-26 2017-06-09 济南大学 基于核酸适配体检测链霉素的生物传感器及其制备方法
AU2016296594B2 (en) 2015-07-17 2020-07-09 Luminex Corporation Methods and compositions for catalytic assays
CN105567839B (zh) * 2016-02-03 2019-02-22 青岛大学 基于网络型核酸纳米探针的酶循环放大检测dna的比色法
US10465233B2 (en) 2016-03-25 2019-11-05 The Government Of The United States Of America, As Represented By The Secretary Of The Navy Time-resolved nucleic acid hybridization beacons
KR20180072480A (ko) * 2016-12-21 2018-06-29 한국생명공학연구원 자가 형광 신호 증폭이 가능한 마이크로 rna 검출용 바이오 프로브 세트 및 이의 용도
CN108728430B (zh) * 2017-04-21 2022-04-05 胤安国际（辽宁）基因科技股份有限公司 一种制备含有多个重复单元dna长探针的方法
CN107884565B (zh) * 2017-10-13 2019-12-24 广东省生态环境技术研究所 一种砷离子的检测方法及检测试剂盒
CN109975561B (zh) * 2019-04-25 2021-11-09 河北医科大学 一种基于核酸适配体的超灵敏检测多巴胺的方法

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5424413A (en) * 1992-01-22 1995-06-13 Gen-Probe Incorporated Branched nucleic acid probes
US20080220425A1 (en) * 1996-01-24 2008-09-11 Third Wave Technologies, Inc. Methods and Compositions for Detecting Target Sequences
WO2007041774A1 (en) 2005-10-07 2007-04-19 Johnson & Johnson Research Pty Limited Multicomponent nucleic acid enzymes and methods for their use
AU2007304837B2 (en) 2006-10-06 2011-09-29 Speedx Pty Ltd Molecular switches and methods for their use
US20120058471A1 (en) * 2007-08-13 2012-03-08 University Of Strathclyde Identification of nucleic acid sequences
WO2010017246A1 (en) * 2008-08-04 2010-02-11 University Of Maryland Probes and methods for detecting analytes
US8278048B2 (en) * 2008-08-15 2012-10-02 Cascade Biosystems, Inc. Detecting nucleic acid
WO2012065231A1 (en) 2010-11-19 2012-05-24 Speedx Pty Ltd Signal amplification

Also Published As

Publication number Publication date
KR20140026343A (ko) 2014-03-05
SG10201509557XA (en) 2015-12-30
US20140017669A1 (en) 2014-01-16
MX2013005569A (es) 2014-02-03
WO2012065231A1 (en) 2012-05-24
EP2640852A4 (en) 2014-05-14
CN103328655A (zh) 2013-09-25
JP2013542738A (ja) 2013-11-28
IL226389A0 (en) 2013-07-31
KR101968352B1 (ko) 2019-04-11
EP2640852B1 (en) 2018-08-22
BR112013012450B1 (pt) 2020-10-13
AU2011331920B2 (en) 2014-10-30
EP2640852A1 (en) 2013-09-25
ZA201303552B (en) 2014-01-29
JP6092114B2 (ja) 2017-03-08
BR112013012450A2 (pt) 2016-08-30
AU2011331920A1 (en) 2013-05-02
IL226389A (en) 2016-03-31
CA2818478C (en) 2020-07-21
CA2818478A1 (en) 2012-05-24
NZ610584A (en) 2015-02-27
SG190841A1 (en) 2013-07-31
US9862990B2 (en) 2018-01-09

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