CN107884565B - 一种砷离子的检测方法及检测试剂盒 - Google Patents
一种砷离子的检测方法及检测试剂盒 Download PDFInfo
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- CN107884565B CN107884565B CN201710951750.0A CN201710951750A CN107884565B CN 107884565 B CN107884565 B CN 107884565B CN 201710951750 A CN201710951750 A CN 201710951750A CN 107884565 B CN107884565 B CN 107884565B
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- 238000001514 detection method Methods 0.000 title claims abstract description 51
- 229910052785 arsenic Inorganic materials 0.000 title claims abstract description 13
- -1 arsenic ions Chemical class 0.000 title claims abstract description 11
- 108010042407 Endonucleases Proteins 0.000 claims abstract description 17
- 102000004533 Endonucleases Human genes 0.000 claims abstract description 17
- 238000006243 chemical reaction Methods 0.000 claims abstract description 14
- 230000000295 complement effect Effects 0.000 claims description 17
- 108020004707 nucleic acids Proteins 0.000 claims description 10
- 102000039446 nucleic acids Human genes 0.000 claims description 10
- 150000007523 nucleic acids Chemical class 0.000 claims description 10
- 238000010791 quenching Methods 0.000 claims description 9
- 230000000171 quenching effect Effects 0.000 claims description 9
- 239000000243 solution Substances 0.000 claims description 8
- 108091023037 Aptamer Proteins 0.000 claims description 5
- HAYXDMNJJFVXCI-UHFFFAOYSA-N arsenic(5+) Chemical compound [As+5] HAYXDMNJJFVXCI-UHFFFAOYSA-N 0.000 claims description 5
- 239000000872 buffer Substances 0.000 claims description 5
- 239000007853 buffer solution Substances 0.000 claims description 4
- 108091008146 restriction endonucleases Proteins 0.000 claims description 2
- 230000009870 specific binding Effects 0.000 claims description 2
- 238000000034 method Methods 0.000 abstract description 8
- 230000003321 amplification Effects 0.000 abstract description 5
- 238000003199 nucleic acid amplification method Methods 0.000 abstract description 5
- 230000007613 environmental effect Effects 0.000 abstract description 3
- 229910001385 heavy metal Inorganic materials 0.000 abstract description 3
- 230000035945 sensitivity Effects 0.000 abstract description 3
- 238000004458 analytical method Methods 0.000 abstract 1
- 238000001917 fluorescence detection Methods 0.000 abstract 1
- 108091008104 nucleic acid aptamers Proteins 0.000 abstract 1
- 108020004414 DNA Proteins 0.000 description 16
- 102000053602 DNA Human genes 0.000 description 6
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 6
- QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical group Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 description 5
- 102000004190 Enzymes Human genes 0.000 description 5
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- 239000012086 standard solution Substances 0.000 description 5
- TWRXJAOTZQYOKJ-UHFFFAOYSA-L Magnesium chloride Chemical compound [Mg+2].[Cl-].[Cl-] TWRXJAOTZQYOKJ-UHFFFAOYSA-L 0.000 description 4
- 150000002500 ions Chemical class 0.000 description 3
- 239000011780 sodium chloride Substances 0.000 description 3
- RQNWIZPPADIBDY-UHFFFAOYSA-N arsenic atom Chemical compound [As] RQNWIZPPADIBDY-UHFFFAOYSA-N 0.000 description 2
- 238000003776 cleavage reaction Methods 0.000 description 2
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- 230000000875 corresponding effect Effects 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 238000001676 hydride generation atomic fluorescence spectroscopy Methods 0.000 description 2
- 229910001629 magnesium chloride Inorganic materials 0.000 description 2
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- 238000004445 quantitative analysis Methods 0.000 description 2
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- 230000007670 carcinogenicity Effects 0.000 description 1
- 231100000260 carcinogenicity Toxicity 0.000 description 1
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- ABZLKHKQJHEPAX-UHFFFAOYSA-N tetramethylrhodamine Chemical compound C=12C=CC(N(C)C)=CC2=[O+]C2=CC(N(C)C)=CC=C2C=1C1=CC=CC=C1C([O-])=O ABZLKHKQJHEPAX-UHFFFAOYSA-N 0.000 description 1
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Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/5308—Immunoassay; Biospecific binding assay; Materials therefor for analytes not provided for elsewhere, e.g. nucleic acids, uric acid, worms, mites
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Molecular Biology (AREA)
- Immunology (AREA)
- Engineering & Computer Science (AREA)
- Hematology (AREA)
- Chemical & Material Sciences (AREA)
- Biomedical Technology (AREA)
- Urology & Nephrology (AREA)
- Microbiology (AREA)
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- Biotechnology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
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CN107884565A CN107884565A (zh) | 2018-04-06 |
CN107884565B true CN107884565B (zh) | 2019-12-24 |
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Families Citing this family (3)
Publication number | Priority date | Publication date | Assignee | Title |
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CN109055492B (zh) * | 2018-07-31 | 2022-02-22 | 广东省生态环境技术研究所 | 一种免标记荧光检测铅离子的方法及检测试剂盒 |
CN113295857B (zh) * | 2020-02-22 | 2024-11-01 | 重庆博视知识产权服务有限公司 | 一种同时检测As3+、Pb2+和Hg2+的方法 |
CN113295657B (zh) * | 2020-02-22 | 2024-12-31 | 青岛科技大学 | 一种同时检测As3+、Pb2+和Hg2+的纳米胶囊-核酸分子复合物及其制备方法 |
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US9862990B2 (en) * | 2010-11-19 | 2018-01-09 | Speedx Pty Ltd | Signal amplification |
CN105018626B (zh) * | 2015-08-06 | 2018-12-25 | 常熟理工学院 | 一种确定样品中砷浓度的方法 |
CN105624165B (zh) * | 2016-01-05 | 2018-11-30 | 山东大学 | 基于自锁式适体探针的级联扩增策略的生物分子检测方法 |
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2017
- 2017-10-13 CN CN201710951750.0A patent/CN107884565B/zh active Active
Non-Patent Citations (4)
Title |
---|
A universal amplified strategy for aptasensors: Enhancing sensitivity through allostery-triggered enzymatic recycling amplification;Kejun Feng等;《Biosensors and Bioelectronics》;20121231;第38卷(第1期);121-125 * |
Selective and sensitive mercuric (ii) ion detection based on quantum dots and nicking endonuclease assisted signal amplification;Jing Ma等;《Biosensors and Bioelectronics》;20130515;第43卷;84-87 * |
基于发卡探针DNA/G-四链体模拟酶结构转化的高灵敏无标记荧光检测DNA;宋璐娜等;《分析化学》;20150915(第9期);1402-1407 * |
基于适配体茎环开关和链置换放大技术的铅离子荧光适配体传感器;王开宇等;《第六届中国临床微生物学大会暨微生物学与免疫学论坛论文汇编》;20150903;208-209 * |
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