ATE332398T1 - Einfach markierte oligonukleotidsonden - Google Patents
Einfach markierte oligonukleotidsondenInfo
- Publication number
- ATE332398T1 ATE332398T1 AT01959722T AT01959722T ATE332398T1 AT E332398 T1 ATE332398 T1 AT E332398T1 AT 01959722 T AT01959722 T AT 01959722T AT 01959722 T AT01959722 T AT 01959722T AT E332398 T1 ATE332398 T1 AT E332398T1
- Authority
- AT
- Austria
- Prior art keywords
- probes
- nucleic acid
- methods
- labeled oligonucleotide
- oligonucleotide probe
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
- C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6851—Quantitative amplification
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Immunology (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Analytical Chemistry (AREA)
- Biotechnology (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biophysics (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Saccharide Compounds (AREA)
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US22472600P | 2000-08-11 | 2000-08-11 | |
US24061000P | 2000-10-16 | 2000-10-16 |
Publications (1)
Publication Number | Publication Date |
---|---|
ATE332398T1 true ATE332398T1 (de) | 2006-07-15 |
Family
ID=26918967
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
AT01959722T ATE332398T1 (de) | 2000-08-11 | 2001-08-10 | Einfach markierte oligonukleotidsonden |
Country Status (8)
Country | Link |
---|---|
US (1) | US6635427B2 (de) |
EP (1) | EP1307592B1 (de) |
JP (1) | JP5213297B2 (de) |
AT (1) | ATE332398T1 (de) |
CA (1) | CA2417986C (de) |
DE (1) | DE60121342T2 (de) |
ES (1) | ES2267803T3 (de) |
WO (1) | WO2002014555A2 (de) |
Families Citing this family (93)
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DE10027113A1 (de) * | 1999-12-23 | 2001-09-27 | Andreas Hoeft | Verfahren zur Bestimmung von mikrobieller DNS/RNS, Kit dafür und Verwendung des Verfahrens |
FI20000333A0 (fi) * | 2000-02-16 | 2000-02-16 | Jussi Nurmi | Homogeeninen menetelmä polynukleotidin havaitsemiseksi |
US7998673B2 (en) * | 2000-03-29 | 2011-08-16 | Lgc Limited | Hybridisation beacon and method of rapid sequence detection and discrimination |
PT1715063E (pt) * | 2000-03-29 | 2011-04-28 | Lgc Ltd | Farol de hibridização e método para a detecção e discriminação rápida de sequência |
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US7785776B2 (en) * | 2002-05-13 | 2010-08-31 | Idaho Technology, Inc. | Genotyping by amplicon melting curve analysis |
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CA2433473A1 (en) * | 2002-07-23 | 2004-01-23 | F. Hoffmann-La Roche Ag | Fluorescent hybridization probes with reduced background |
US7122384B2 (en) * | 2002-11-06 | 2006-10-17 | E. I. Du Pont De Nemours And Company | Resonant light scattering microparticle methods |
US20060099596A1 (en) | 2002-12-06 | 2006-05-11 | Roche Molecular Systems, Inc. | Multiplex assay detection of pathogenic organisms |
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EP1613771B1 (de) | 2003-04-04 | 2012-03-21 | Roche Diagnostics GmbH | Verbessertes system für mehrfarbige echtzeit-pcr |
JP4454249B2 (ja) | 2003-04-18 | 2010-04-21 | アークレイ株式会社 | 膵ラ氏島アミロイドタンパク質変異遺伝子の検出法ならびにそのための核酸プローブおよびキット |
US20050053950A1 (en) * | 2003-09-08 | 2005-03-10 | Enrique Zudaire Ubani | Protocol and software for multiplex real-time PCR quantification based on the different melting temperatures of amplicons |
CN101076537B (zh) * | 2003-11-19 | 2012-07-04 | 阿莱洛吉克生物科学公司 | 标记有多个荧光团的寡核苷酸 |
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EP1634965B1 (de) | 2004-09-09 | 2010-01-20 | Roche Diagnostics GmbH | Echtzeit-PCR unter Zusatz von Pyrophosphatase |
EP1841890B1 (de) * | 2005-01-03 | 2013-08-14 | THE GOVERNMENT OF THE UNITED STATES OF AMERICA, as represented by the Secretary, Department of Health and Human Services | Primer für nukleinsäurenachweis |
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EP1700922B1 (de) | 2005-03-10 | 2016-08-24 | Roche Diagnostics GmbH | 3-Substituierte 5-Nitroindolderivate und diese enthaltende markierte Oligonukleotidsonden |
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US7781165B2 (en) | 2005-10-19 | 2010-08-24 | Roche Diagnostics Operations, Inc. | Benzimidazolium compounds and salts of benzimidazolium compounds for nucleic acid amplification |
DE502005008153D1 (de) | 2005-11-23 | 2009-10-29 | Roche Diagnostics Gmbh | Polynukleotid mit Phosphatmimetikum |
JP4713514B2 (ja) * | 2006-02-20 | 2011-06-29 | エフ.ホフマン−ラ ロシュ アーゲー | 改善された標識試薬 |
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RU2394915C2 (ru) * | 2006-03-24 | 2010-07-20 | Александр Борисович Четверин | Бесконтактные способы обнаружения молекулярных колоний, наборы реагентов и устройство для их осуществления |
WO2008020090A1 (es) * | 2006-08-18 | 2008-02-21 | Laboratorios Indas, S.A. | Método y kit para diagnosticar el riesgo de desarrollar una enfermedad trombótica venosa |
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CN102449167B (zh) | 2009-05-26 | 2014-12-17 | 厦门大学 | 一种检测核酸序列变异的方法 |
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JP5635496B2 (ja) * | 2009-10-30 | 2014-12-03 | アークレイ株式会社 | Egfr遺伝子多型検出用プローブおよびその用途 |
WO2011062258A1 (ja) * | 2009-11-19 | 2011-05-26 | アークレイ株式会社 | Mthfr遺伝子増幅用プライマーセット、それを含むmthfr遺伝子増幅用試薬およびその用途 |
JP5917144B2 (ja) * | 2009-12-07 | 2016-05-11 | アークレイ株式会社 | 疾患関連遺伝子の多型検出用プローブおよびその用途 |
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JP2020526194A (ja) | 2017-06-29 | 2020-08-31 | ジュノー セラピューティクス インコーポレイテッド | 免疫療法薬と関連する毒性を評価するためのマウスモデル |
WO2019027850A1 (en) | 2017-07-29 | 2019-02-07 | Juno Therapeutics, Inc. | CELL EXPANSION REAGENTS EXPRESSING RECOMBINANT RECEPTORS |
MA50057A (fr) | 2017-09-01 | 2020-07-08 | Juno Therapeutics Inc | Expression génique et évaluation d'un risque de développement d'une toxicité suite à une thérapie cellulaire |
CN107727618B (zh) * | 2017-09-04 | 2019-12-31 | 江苏大学 | 生物芯片和特异性切割位点dna序列检测汞离子的方法 |
MX2020004239A (es) | 2017-11-01 | 2020-09-09 | Juno Therapeutics Inc | Proceso para producir una composicion de celulas t. |
CN111918972A (zh) | 2018-01-31 | 2020-11-10 | 朱诺治疗学股份有限公司 | 评估存在或不存在有复制能力的病毒的方法和试剂 |
GB201812192D0 (en) | 2018-07-26 | 2018-09-12 | Ttp Plc | Variable temperature reactor, heater and control circuit for the same |
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CN114302736A (zh) | 2019-04-17 | 2022-04-08 | 高山免疫科学股份有限公司 | 变体icos配体(icosl)融合蛋白的方法和用途 |
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KR102107589B1 (ko) * | 2019-11-20 | 2020-05-07 | 주식회사 유진셀 | 단일 신호 형광 물질을 이용한 실시간 타겟 핵산 검출 및 정량 방법 |
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WO1998026093A2 (en) * | 1996-12-13 | 1998-06-18 | The Government Of The United States Of America, Represented By The Secretary, Department Of Health And Human Services | Fluorescent nucleotide analog hairpin formation for detection of nucleic acid hybridization |
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DE19858588B4 (de) | 1998-08-22 | 2016-04-07 | Qiagen Gmbh | Farbstoffmarkiertes Oligonukleotid zum Markieren eines Nukleinsäuremoleküls |
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-
2001
- 2001-08-10 AT AT01959722T patent/ATE332398T1/de not_active IP Right Cessation
- 2001-08-10 ES ES01959722T patent/ES2267803T3/es not_active Expired - Lifetime
- 2001-08-10 DE DE60121342T patent/DE60121342T2/de not_active Expired - Lifetime
- 2001-08-10 CA CA2417986A patent/CA2417986C/en not_active Expired - Lifetime
- 2001-08-10 WO PCT/US2001/025231 patent/WO2002014555A2/en active IP Right Grant
- 2001-08-10 JP JP2002519680A patent/JP5213297B2/ja not_active Expired - Lifetime
- 2001-08-10 EP EP01959722A patent/EP1307592B1/de not_active Expired - Lifetime
- 2001-08-10 US US09/927,842 patent/US6635427B2/en not_active Expired - Lifetime
Also Published As
Publication number | Publication date |
---|---|
DE60121342T2 (de) | 2007-06-28 |
EP1307592B1 (de) | 2006-07-05 |
DE60121342D1 (de) | 2006-08-17 |
US20030022177A1 (en) | 2003-01-30 |
ES2267803T3 (es) | 2007-03-16 |
EP1307592A2 (de) | 2003-05-07 |
JP2004506431A (ja) | 2004-03-04 |
WO2002014555A2 (en) | 2002-02-21 |
JP5213297B2 (ja) | 2013-06-19 |
CA2417986A1 (en) | 2002-02-21 |
CA2417986C (en) | 2013-11-26 |
US6635427B2 (en) | 2003-10-21 |
WO2002014555A3 (en) | 2003-02-27 |
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