ATE491041T1 - Verfahren zur quantifizierung von methylierter dns - Google Patents
Verfahren zur quantifizierung von methylierter dnsInfo
- Publication number
- ATE491041T1 ATE491041T1 AT04090255T AT04090255T ATE491041T1 AT E491041 T1 ATE491041 T1 AT E491041T1 AT 04090255 T AT04090255 T AT 04090255T AT 04090255 T AT04090255 T AT 04090255T AT E491041 T1 ATE491041 T1 AT E491041T1
- Authority
- AT
- Austria
- Prior art keywords
- dna
- real time
- methylated dna
- methylated
- converted
- Prior art date
Links
- 238000000034 method Methods 0.000 title abstract 3
- ISAKRJDGNUQOIC-UHFFFAOYSA-N Uracil Chemical compound O=C1C=CNC(=O)N1 ISAKRJDGNUQOIC-UHFFFAOYSA-N 0.000 abstract 2
- 230000011987 methylation Effects 0.000 abstract 2
- 238000007069 methylation reaction Methods 0.000 abstract 2
- LRSASMSXMSNRBT-UHFFFAOYSA-N 5-methylcytosine Chemical class CC1=CNC(=O)N=C1N LRSASMSXMSNRBT-UHFFFAOYSA-N 0.000 abstract 1
- 230000002411 adverse Effects 0.000 abstract 1
- 230000024245 cell differentiation Effects 0.000 abstract 1
- 238000003745 diagnosis Methods 0.000 abstract 1
- 238000004393 prognosis Methods 0.000 abstract 1
- 238000011002 quantification Methods 0.000 abstract 1
- 238000003753 real-time PCR Methods 0.000 abstract 1
- 239000000523 sample Substances 0.000 abstract 1
- 229940035893 uracil Drugs 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6858—Allele-specific amplification
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T436/00—Chemistry: analytical and immunological testing
- Y10T436/14—Heterocyclic carbon compound [i.e., O, S, N, Se, Te, as only ring hetero atom]
- Y10T436/142222—Hetero-O [e.g., ascorbic acid, etc.]
- Y10T436/143333—Saccharide [e.g., DNA, etc.]
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Saccharide Compounds (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP04090255A EP1609873B1 (de) | 2004-06-23 | 2004-06-23 | Verfahren zur Quantifizierung von methylierter DNS |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE491041T1 true ATE491041T1 (de) | 2010-12-15 |
Family
ID=34928810
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT04090255T ATE491041T1 (de) | 2004-06-23 | 2004-06-23 | Verfahren zur quantifizierung von methylierter dns |
Country Status (5)
| Country | Link |
|---|---|
| US (1) | US7972784B2 (de) |
| EP (1) | EP1609873B1 (de) |
| AT (1) | ATE491041T1 (de) |
| DE (1) | DE602004030430D1 (de) |
| WO (1) | WO2006000447A1 (de) |
Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US10731215B2 (en) | 2005-04-15 | 2020-08-04 | Epigenomics Ag | Method for determining the presence or absence of methylation in a sample |
| JP2010199747A (ja) * | 2009-02-23 | 2010-09-09 | Canon Inc | 画像処理装置、画像処理装置の空間周波数変換方法、及びプログラム |
| CN102321745B (zh) * | 2010-10-14 | 2014-05-14 | 博尔诚（北京）科技有限公司 | 一种定量检测dna特定位点甲基化程度的方法及试剂盒 |
| CN105335607B (zh) * | 2015-10-12 | 2017-06-16 | 湖北工业大学 | 一种边坡渐进破坏潜在滑动面的计算方法 |
| AU2016383531B2 (en) * | 2015-12-30 | 2021-02-25 | Technische Universität Dresden | Method and means for diagnosing tumors |
| US10913986B2 (en) | 2016-02-01 | 2021-02-09 | The Board Of Regents Of The University Of Nebraska | Method of identifying important methylome features and use thereof |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6331393B1 (en) * | 1999-05-14 | 2001-12-18 | University Of Southern California | Process for high-throughput DNA methylation analysis |
| DE10112515B4 (de) * | 2001-03-09 | 2004-02-12 | Epigenomics Ag | Verfahren zum Nachweis von Cytosin-Methylierungsmustern mit hoher Sensitivität |
| JP4917891B2 (ja) * | 2003-10-21 | 2012-04-18 | オリオン ゲノミクス エルエルシー | 差次的酵素的断片化の方法 |
| EP1692264A2 (de) * | 2003-10-28 | 2006-08-23 | The Johns Hopkins University | Quantitative methylierungsspezifische multiplex-pcr |
| US20050287553A1 (en) * | 2004-04-06 | 2005-12-29 | Epigenomics Ag | Method for the quantification of methylated DNA |
-
2004
- 2004-06-23 AT AT04090255T patent/ATE491041T1/de not_active IP Right Cessation
- 2004-06-23 DE DE602004030430T patent/DE602004030430D1/de not_active Expired - Lifetime
- 2004-06-23 EP EP04090255A patent/EP1609873B1/de not_active Expired - Lifetime
-
2005
- 2005-06-23 US US11/630,659 patent/US7972784B2/en not_active Expired - Fee Related
- 2005-06-23 WO PCT/EP2005/006907 patent/WO2006000447A1/en active Application Filing
Also Published As
| Publication number | Publication date |
|---|---|
| EP1609873A1 (de) | 2005-12-28 |
| US7972784B2 (en) | 2011-07-05 |
| WO2006000447A1 (en) | 2006-01-05 |
| US20080311566A1 (en) | 2008-12-18 |
| EP1609873B1 (de) | 2010-12-08 |
| DE602004030430D1 (de) | 2011-01-20 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| RER | Ceased as to paragraph 5 lit. 3 law introducing patent treaties |