GB2362212A - Method for the identification and speciation of bacteria of the burkholderia cepacia complex - Google Patents
Method for the identification and speciation of bacteria of the burkholderia cepacia complexInfo
- Publication number
- GB2362212A GB2362212A GB0104647A GB0104647A GB2362212A GB 2362212 A GB2362212 A GB 2362212A GB 0104647 A GB0104647 A GB 0104647A GB 0104647 A GB0104647 A GB 0104647A GB 2362212 A GB2362212 A GB 2362212A
- Authority
- GB
- United Kingdom
- Prior art keywords
- bacteria
- burkholderia cepacia
- complex
- sequence information
- nucleotide sequence
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
- C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/04—Antibacterial agents
Abstract
Identification and speciation of bacteria of the <I>Burkholderia cepacia</I> complex in a sample can be accomplished by: a) obtaining nucleotide sequence information for the <I>recA</I> gene in bacteria of the <I>Burkholderia cepacia</I> complex found in the sample; and b) comparing the nucleotide sequence information obtained for the <I>recA</I> gene in bacteria of the <I>Burkholderia cepacia</I> complex found in the sample with a standard library of nucleotide sequence information comprising standard nucleotide sequence information for at least three species of bacteria of the <I>Burkholderia cepacia</I> complex. Preferably, the nucleotide sequence information is obtained by evaluation of restriction fragment length polymorphism (RFLP). Other techniques for obtaining sequence information can also be used, including base-by-base determination of the sequence of the region of interest, sequence-specific oligonucleotide hybridization probes, and ligation techniques. Universal primers which can be used for amplification of all known members of the <I>Burkholderia cepacia</I> complex, and genomovar-specific primers which can be used for selective amplification of the <I>recA</I> gene from bacteria of one genomovar provide alternative analytical modalities. Speciation of bacteria of the <I>Burkholderia cepacia</I> complex can be used as a basis for administration of a vaccine specific to the flagellin of the bacteria, since it is shown that this flagellin is conserved across members of genomovar III, subgroup RG-B.
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US9911698P | 1998-09-03 | 1998-09-03 | |
US9911598P | 1998-09-03 | 1998-09-03 | |
PCT/CA1999/000813 WO2000014274A1 (en) | 1998-09-03 | 1999-09-03 | Method for the identification and speciation of bacteria of the burkholderia cepacia complex |
Publications (2)
Publication Number | Publication Date |
---|---|
GB0104647D0 GB0104647D0 (en) | 2001-04-11 |
GB2362212A true GB2362212A (en) | 2001-11-14 |
Family
ID=26795589
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
GB0104647A Withdrawn GB2362212A (en) | 1998-09-03 | 1999-09-03 | Method for the identification and speciation of bacteria of the burkholderia cepacia complex |
Country Status (3)
Country | Link |
---|---|
AU (1) | AU5612099A (en) |
GB (1) | GB2362212A (en) |
WO (1) | WO2000014274A1 (en) |
Families Citing this family (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20020055101A1 (en) | 1995-09-11 | 2002-05-09 | Michel G. Bergeron | Specific and universal probes and amplification primers to rapidly detect and identify common bacterial pathogens and antibiotic resistance genes from clinical specimens for routine diagnosis in microbiology laboratories |
US20100267012A1 (en) | 1997-11-04 | 2010-10-21 | Bergeron Michel G | Highly conserved genes and their use to generate probes and primers for detection of microorganisms |
US20030049636A1 (en) | 1999-05-03 | 2003-03-13 | Bergeron Michel G. | Species-specific, genus-specific and universal DNA probes and amplification primers to rapidly detect and identify common bacterial and fungal pathogens and associated antibiotic resistance genes from clinical specimens for diagnosis in microbiology laboratories |
CA2905326C (en) | 1999-09-28 | 2016-09-27 | Geneohm Sciences Canada Inc. | Nucleic acids and methods for the detection of klebsiella |
CN100432228C (en) * | 2003-11-21 | 2008-11-12 | 广东省微生物研究所 | Ribotide sequence of 2-naphthanic acid degradation bacteria DNA segment and its preparation method and application |
CN116064866B (en) * | 2022-09-23 | 2023-09-29 | 中国食品药品检定研究院 | Kit, primer, method and application for detecting Burkholderia cepacia group |
Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO1997001647A2 (en) * | 1995-06-28 | 1997-01-16 | Trustees Of Health & Hospitals Of The City Of Boston, Inc. | Dna sequences for identifying highly transmissible lineages of pseudomonas (burkholderia) cepacia |
WO1998020157A2 (en) * | 1996-11-04 | 1998-05-14 | Infectio Diagnostic (I.D.I.) Inc. | Species-specific, genus-specific and universal dna probes and amplification primers to rapidly detect and identify common bacterial and fungal pathogens and associated antibiotic resistance genes from clinical specimens for diagnosis in microbiology laboratories |
-
1999
- 1999-09-03 GB GB0104647A patent/GB2362212A/en not_active Withdrawn
- 1999-09-03 WO PCT/CA1999/000813 patent/WO2000014274A1/en active Application Filing
- 1999-09-03 AU AU56120/99A patent/AU5612099A/en not_active Abandoned
Patent Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO1997001647A2 (en) * | 1995-06-28 | 1997-01-16 | Trustees Of Health & Hospitals Of The City Of Boston, Inc. | Dna sequences for identifying highly transmissible lineages of pseudomonas (burkholderia) cepacia |
WO1998020157A2 (en) * | 1996-11-04 | 1998-05-14 | Infectio Diagnostic (I.D.I.) Inc. | Species-specific, genus-specific and universal dna probes and amplification primers to rapidly detect and identify common bacterial and fungal pathogens and associated antibiotic resistance genes from clinical specimens for diagnosis in microbiology laboratories |
Non-Patent Citations (8)
Title |
---|
INT J SYSTEMIC BACTERIOL, 1997, 47, 1188-1200, P VANDAMME ETAL * |
J BACTERIOLOGY, 1995, 177, 6881-6893, S KARLIN ET AL * |
J BACTERIOLOGY, 1998, 180, 1110-1118, BA HALES ET AL * |
J CLINICAL MICROBIOL, 1994, 32, 2422-2424, SE DASEN ET AL * |
J CLINICAL MICROBIOL, 1995, 33, 3304-3307, P YUK-FONG LIU ETAL * |
J CLINICAL MICROBIOL, 1996, 34, 2914-2920, E MAHENTHIRALINGAM ET AL * |
J MED MICROBIOL, 1995, 43, 436-441, HC RYLEY ET AL * |
PEDIATRIC RES, 35, 394A, JJ LIPUMA ET AL * |
Also Published As
Publication number | Publication date |
---|---|
GB0104647D0 (en) | 2001-04-11 |
WO2000014274A1 (en) | 2000-03-16 |
AU5612099A (en) | 2000-03-27 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
AT | Applications terminated before publication under section 16(1) | ||
WAP | Application withdrawn, taken to be withdrawn or refused ** after publication under section 16(1) | ||
WAP | Application withdrawn, taken to be withdrawn or refused ** after publication under section 16(1) |