DE60216777D1 - Multiplex-hpv-pcr-fluoreszenzassays unter verwendung mehrerer fluorophore - Google Patents

Multiplex-hpv-pcr-fluoreszenzassays unter verwendung mehrerer fluorophore

Info

Publication number
DE60216777D1
DE60216777D1 DE60216777T DE60216777T DE60216777D1 DE 60216777 D1 DE60216777 D1 DE 60216777D1 DE 60216777 T DE60216777 T DE 60216777T DE 60216777 T DE60216777 T DE 60216777T DE 60216777 D1 DE60216777 D1 DE 60216777D1
Authority
DE
Germany
Prior art keywords
fluorescence assays
pcr fluorescence
hpv
present
multiple fluorophore
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE60216777T
Other languages
English (en)
Other versions
DE60216777T2 (de
Inventor
U Jansen
J Taddeo
Weili Li
C Dicello
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Merck and Co Inc
Original Assignee
Merck and Co Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Merck and Co Inc filed Critical Merck and Co Inc
Publication of DE60216777D1 publication Critical patent/DE60216777D1/de
Application granted granted Critical
Publication of DE60216777T2 publication Critical patent/DE60216777T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701—Specific hybridization probes
    • C12Q1/708—Specific hybridization probes for papilloma

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Virology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
DE60216777T 2001-08-23 2002-08-19 Multiplex-hpv-pcr-fluoreszenzassays unter verwendung mehrerer fluorophore Expired - Lifetime DE60216777T2 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US31438301P 2001-08-23 2001-08-23
US314383P 2001-08-23
PCT/US2002/026964 WO2003019143A2 (en) 2001-08-23 2002-08-19 Fluorescent multiplex hpv pcr assays using multiple fluorophores

Publications (2)

Publication Number Publication Date
DE60216777D1 true DE60216777D1 (de) 2007-01-25
DE60216777T2 DE60216777T2 (de) 2007-10-31

Family

ID=23219734

Family Applications (1)

Application Number Title Priority Date Filing Date
DE60216777T Expired - Lifetime DE60216777T2 (de) 2001-08-23 2002-08-19 Multiplex-hpv-pcr-fluoreszenzassays unter verwendung mehrerer fluorophore

Country Status (11)

Country Link
US (1) US20050175987A1 (de)
EP (1) EP1421200B1 (de)
JP (1) JP2005500856A (de)
AT (1) ATE348192T1 (de)
CA (1) CA2457888A1 (de)
CY (1) CY1106353T1 (de)
DE (1) DE60216777T2 (de)
DK (1) DK1421200T3 (de)
ES (1) ES2276948T3 (de)
PT (1) PT1421200E (de)
WO (1) WO2003019143A2 (de)

Families Citing this family (26)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7087414B2 (en) 2000-06-06 2006-08-08 Applera Corporation Methods and devices for multiplexing amplification reactions
WO2003057914A2 (en) * 2002-01-07 2003-07-17 Norchip A/S METHOD FOR DETECTING HUMAN PAPILLOMAVIRUS mRNA
US6818420B2 (en) 2002-02-27 2004-11-16 Biosource International, Inc. Methods of using FET labeled oligonucleotides that include a 3′-5′ exonuclease resistant quencher domain and compositions for practicing the same
JP2006508662A (ja) 2002-12-04 2006-03-16 アプレラ コーポレイション ポリヌクレオチドの多重増幅
CN1294276C (zh) * 2003-07-22 2007-01-10 厦门大学 检测核酸的等长双链特异性探针
JP4999460B2 (ja) * 2003-10-13 2012-08-15 ゲナコ・バイオメデイカル・プロダクツ・インコーポレイテツド 核酸のプライマーに基づく増幅のための方法及びキット
US7901883B2 (en) 2004-12-10 2011-03-08 Genera Biosystems Limited Human papilloma virus (HPV) detection using nucleic acid probes, microbeads and fluorescent-activated cell sorter (FACS)
KR100705746B1 (ko) 2005-03-14 2007-04-09 학교법인 성균관대학 사람파필로마바이러스의 검출을 위한 프라이머
US8927208B2 (en) * 2005-03-30 2015-01-06 Labo Bio-Medical Investments B.V. Identification of beta-papillomavirus DNA by type-specific reverse hybridization
CA2604692A1 (en) * 2005-04-28 2006-11-02 Merck And Co., Inc. Fluorescent multiplex hpv pcr assays
CA2648971A1 (en) * 2006-04-11 2007-10-18 Bio-Rad Pasteur Hpv detection and quantification by real-time multiplex amplification
EP2210955A3 (de) * 2006-05-23 2010-09-15 Molecular Detection, Inc. Bei Umgebungstemperatur beständige Kits zur Molekulardiagnostik
DE102006041970A1 (de) * 2006-08-28 2008-03-06 Genome Identification Diagnostics Gmbh Verfahren und Mittel zum Nachweis von humanen Papillomaviren
ES2403729T3 (es) 2007-04-05 2013-05-21 Genera Biosystems Limited Composiciones y métodos de detección
EP1997914A1 (de) * 2007-06-01 2008-12-03 Università Degli Studi Di Milano - Bicocca Identifizierung und Quantifizierung onkogener HPV-Nukleinsäuren mittels PCR-Assays in Echtzeit
JP5595276B2 (ja) * 2007-11-01 2014-09-24 フェレニヒン フォール クリステレイク ホーヘル オンデルウェイス，ウェーテンスハッペレイク オンデルズーク エン パティエンテンゾルフ 子宮頸部ｈｐｖの新たな検出方法
WO2011088573A1 (en) * 2010-01-19 2011-07-28 Her Majesty The Queen In Right Of Canada As Represented By The Minister Of Health Set of probes for the detection and typing of 46 human papillomavirus mucosal types
JP5965846B2 (ja) 2010-02-16 2016-08-10 ベクトン・ディキンソン・アンド・カンパニーＢｅｃｔｏｎ， Ｄｉｃｋｉｎｓｏｎ Ａｎｄ Ｃｏｍｐａｎｙ 近縁のヒトパピローマウイルス（ｈｐｖ）の血清型を検出するためのアッセイ
CN103517994A (zh) * 2011-01-07 2014-01-15 奇亚根盖瑟斯堡股份有限公司 用于对高风险人乳头瘤病毒进行基因型分型和定量的材料和方法
US10371610B2 (en) 2016-02-23 2019-08-06 Noul Co., Ltd. Contact-type patch, staining method using the same, and manufacturing method thereof
KR20170099738A (ko) 2016-02-23 2017-09-01 노을 주식회사 접촉식 염색 패치 및 그 제조 방법
EP3856860B1 (de) 2018-09-24 2023-11-01 Flooring Industries Limited, SARL Keramiktinten für digitalen tintenstrahldrucker
EP4028559A4 (de) * 2019-08-15 2023-09-20 Shrestha, Devjani Ghosh Verfahren zur diagnose und in-vitro-risikostratifizierung für kopf-hals-karzinom auf der grundlage von exosomalen mrna
WO2022115470A1 (en) * 2020-11-25 2022-06-02 Precigen, Inc. Human papilloma virus vaccines and uses of the same for hpv associated diseases
WO2023159110A1 (en) * 2022-02-16 2023-08-24 The General Hospital Corporation Contact-type patches for staining
WO2023195780A1 (en) * 2022-04-08 2023-10-12 Seegene, Inc. Method for detecting three target nucleic acids in sample

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5876922A (en) * 1985-07-31 1999-03-02 Institute Pasteur Papillomavirus probe and a process for in vitro diagnosis of papillomavirus infections
US5447839A (en) * 1988-09-09 1995-09-05 Hoffmann-La Roche Inc. Detection of human papillomavirus by the polymerase chain reaction
US5863717A (en) * 1989-11-03 1999-01-26 Abbott Laboratories Use of conserved oligonucleotide primers to amplify human papillomavirus DNA sequences
US5538848A (en) * 1994-11-16 1996-07-23 Applied Biosystems Division, Perkin-Elmer Corp. Method for detecting nucleic acid amplification using self-quenching fluorescence probe
US5571673A (en) * 1994-11-23 1996-11-05 Hoffmann-La Roche Inc. Methods for in-solution quenching of fluorescently labeled oligonucleotide probes
DE69739357D1 (de) * 1996-06-04 2009-05-28 Univ Utah Res Found Überwachung der hybridisierung während pcr
US6218105B1 (en) * 1996-07-19 2001-04-17 Kathleen S. Hall High throughput papilloma virus in vitro infectivity assay
US6261771B1 (en) * 1998-02-18 2001-07-17 The United States Of America As Represented By The Secretary Of The Army Method and apparatus for detection of multiple nucleic acid sequences and multiple antigens
US6268143B1 (en) * 1998-08-05 2001-07-31 Kansas State University Research Foundation Automated high throughput E. coli o157:H7 PCR detection system and uses thereof

Also Published As

Publication number Publication date
DK1421200T3 (da) 2007-04-10
JP2005500856A (ja) 2005-01-13
ES2276948T3 (es) 2007-07-01
EP1421200A2 (de) 2004-05-26
CY1106353T1 (el) 2011-10-12
WO2003019143A3 (en) 2003-05-08
CA2457888A1 (en) 2003-03-06
EP1421200A4 (de) 2004-12-01
DE60216777T2 (de) 2007-10-31
ATE348192T1 (de) 2007-01-15
PT1421200E (pt) 2007-02-28
US20050175987A1 (en) 2005-08-11
EP1421200B1 (de) 2006-12-13
WO2003019143A2 (en) 2003-03-06

Similar Documents

Publication Publication Date Title
DE60216777D1 (de) Multiplex-hpv-pcr-fluoreszenzassays unter verwendung mehrerer fluorophore
ATE541057T1 (de) Primer und sonden für den nachweis und die unterscheidung von typen und subtypen des influenzavirus
HRP20100414T1 (hr) Postupak detekcije mutacija egfr u uzorcima krvi
ATE448327T1 (de) Verfahren und zusammensetzungen zum nachweis und zur analyse von polynukleotidbindungsproteinwechselwirkungen unter verwendung licht sammelnder multichromophoren
DE60036307D1 (de) Fluoreszenzintensitätstest für duplex- und triplex-nukleinsäurehybridisierung in lösung unter verwendung von fluoreszenzinterkalatoren
ATE476521T1 (de) Verfahren und zusammensetzungen zum nachweis und zur analyse von polynukleotiden unter verwendung von licht einfangenden multichromophoren
ES2659798T3 (es) Detección de la restricción de cadena ligera de inmunoglobulina por hibridación in situ de ARN
WO2004099434A3 (en) Homogeneous multiplex screening assays and kits
US20050112601A1 (en) Methods for cellular or microorganism capture and quantification using bioluminescence regenerative cycle (BRC) assays
WO2006014351A3 (en) Exploring fluorophore microenvironments
US20170097345A1 (en) Assay plate and uses thereof
NZ598954A (en) Diagnosis of metastatic melanoma and monitoring indicators of immunosuppression through blood leukocyte microarray analysis
EP1806410A3 (de) Floureszente, multiplexe HPV-PCR-Assays unter Verwendung multipler Fluorophore
EP1671118A4 (de) Cyanid wahrnehmende verbindungen und verwendungen davon
WO2005083394A3 (en) Methods for detecting anionic and non-anionic proteins using carbocyanine dyes
ES2575538T3 (es) Ensayo para Trichomonas vaginalis mediante amplificación y detección del gen AP65-1 de Trichomonas vaginalis
WO2001068913A3 (en) Nucleic acid detection method and system
NZ596252A (en) A method for detection of human papillomavirus (hpv) type
ES2665465T3 (es) Ensayo para detectar serotipos estrechamente relacionados del papilomavirus humano (HPV)
MY149655A (en) Detection and enumeration of microorganisms
ATE449868T1 (de) Methoden, testsätze und kompositionen zur detektion und quantifizierung von zielsequenzen
CN102071247B8 (zh) 一种区分非嗜肺与嗜肺军团菌的检测方法及试剂盒
ATE259424T1 (de) Enzym-substrate und assay mit intramolekularen fret
WO2006116303A3 (en) Fluorescent multiplex hpv pcr assays
WO2012070863A2 (ko) 핵산효소-분자비콘을 이용한 핵산의 검출방법

Legal Events

Date Code Title Description
8364 No opposition during term of opposition
8327 Change in the person/name/address of the patent owner

Owner name: MERCK & CO., INC., RAHWAY, N.J., US