ATE550099T1 - Verbessertes system für mehrfarbige echtzeit-pcr - Google Patents
Verbessertes system für mehrfarbige echtzeit-pcrInfo
- Publication number
- ATE550099T1 ATE550099T1 AT04725021T AT04725021T ATE550099T1 AT E550099 T1 ATE550099 T1 AT E550099T1 AT 04725021 T AT04725021 T AT 04725021T AT 04725021 T AT04725021 T AT 04725021T AT E550099 T1 ATE550099 T1 AT E550099T1
- Authority
- AT
- Austria
- Prior art keywords
- time pcr
- fret
- improved system
- color real
- directed
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6851—Quantitative amplification
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N2021/6417—Spectrofluorimetric devices
- G01N2021/6421—Measuring at two or more wavelengths
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Applications Claiming Priority (4)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
EP03007458 | 2003-04-04 | ||
EP03014929 | 2003-07-01 | ||
EP03017561 | 2003-08-07 | ||
PCT/EP2004/003457 WO2004087950A2 (en) | 2003-04-04 | 2004-04-01 | Improved system for multi color real time pcr |
Publications (1)
Publication Number | Publication Date |
---|---|
ATE550099T1 true ATE550099T1 (de) | 2012-04-15 |
Family
ID=33135519
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
AT04725021T ATE550099T1 (de) | 2003-04-04 | 2004-04-01 | Verbessertes system für mehrfarbige echtzeit-pcr |
Country Status (7)
Country | Link |
---|---|
US (2) | US8137616B2 (de) |
EP (2) | EP1613771B1 (de) |
JP (2) | JP2006521092A (de) |
AT (1) | ATE550099T1 (de) |
CA (1) | CA2525482C (de) |
ES (1) | ES2384170T3 (de) |
WO (1) | WO2004087950A2 (de) |
Families Citing this family (65)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US7439016B1 (en) | 2000-06-15 | 2008-10-21 | Digene Corporation | Detection of nucleic acids by type-specific hybrid capture method |
US7601497B2 (en) | 2000-06-15 | 2009-10-13 | Qiagen Gaithersburg, Inc. | Detection of nucleic acids by target-specific hybrid capture method |
JP2006521092A (ja) * | 2003-04-04 | 2006-09-21 | エフ.ホフマン−ラ ロシュ アーゲー | マルチカラーリアルタイムpcr用の改良されたシステム |
EP1930730B1 (de) * | 2005-03-10 | 2019-08-14 | Gen-Probe Incorporated | Systeme und Verfahren zur Durchführung von Tests zum Nachweis oder zur Quantifizierung von Analyten |
US8615368B2 (en) | 2005-03-10 | 2013-12-24 | Gen-Probe Incorporated | Method for determining the amount of an analyte in a sample |
US7709249B2 (en) | 2005-04-01 | 2010-05-04 | 3M Innovative Properties Company | Multiplex fluorescence detection device having fiber bundle coupling multiple optical modules to a common detector |
US7507575B2 (en) | 2005-04-01 | 2009-03-24 | 3M Innovative Properties Company | Multiplex fluorescence detection device having removable optical modules |
US7527763B2 (en) | 2005-07-05 | 2009-05-05 | 3M Innovative Properties Company | Valve control system for a rotating multiplex fluorescence detection device |
EP1746169B1 (de) * | 2005-07-21 | 2009-12-23 | Epigenomics AG | Verfahren zur Quantifizierung methylierter DNA |
US7754148B2 (en) | 2006-12-27 | 2010-07-13 | Progentech Limited | Instrument for cassette for sample preparation |
US7727473B2 (en) | 2005-10-19 | 2010-06-01 | Progentech Limited | Cassette for sample preparation |
US11001881B2 (en) | 2006-08-24 | 2021-05-11 | California Institute Of Technology | Methods for detecting analytes |
US8637436B2 (en) | 2006-08-24 | 2014-01-28 | California Institute Of Technology | Integrated semiconductor bioarray |
US7629124B2 (en) | 2006-06-30 | 2009-12-08 | Canon U.S. Life Sciences, Inc. | Real-time PCR in micro-channels |
US8048626B2 (en) | 2006-07-28 | 2011-11-01 | California Institute Of Technology | Multiplex Q-PCR arrays |
US11525156B2 (en) | 2006-07-28 | 2022-12-13 | California Institute Of Technology | Multiplex Q-PCR arrays |
US11560588B2 (en) | 2006-08-24 | 2023-01-24 | California Institute Of Technology | Multiplex Q-PCR arrays |
JP5367365B2 (ja) * | 2006-11-30 | 2013-12-11 | アークレイ株式会社 | Sult1a1遺伝子増幅用プライマーセット、それを含むsult1a1遺伝子増幅用試薬およびその用途 |
CA2722484A1 (en) | 2008-04-24 | 2009-10-29 | Peter D. Ludowise | Analysis of nucleic acid amplification curves using wavelet transformation |
EP3085794B1 (de) * | 2008-10-15 | 2020-03-18 | AXOLABS GmbH | Oligonukleotid-detektionsverfahren |
US8877436B2 (en) | 2008-10-27 | 2014-11-04 | Qiagen Gaithersburg, Inc. | Fast results hybrid capture assay on an automated platform |
CN102625850B (zh) * | 2009-04-03 | 2014-11-26 | 蒂莫西·Z·刘 | 多重核酸检测方法和系统 |
AU2010237532B2 (en) * | 2009-04-15 | 2014-11-20 | Biocartis Nv | Optical detection system for monitoring rtPCR reaction |
CA2760542A1 (en) | 2009-05-01 | 2010-11-04 | Qiagen Gaithersburg, Inc. | A non-target amplification method for detection of rna splice-forms in a sample |
CN102459649A (zh) * | 2009-05-29 | 2012-05-16 | 肺结核诊断公司 | 先进的病原体检测和筛选 |
JP5826752B2 (ja) | 2009-09-14 | 2015-12-02 | キアジェン ゲイサーズバーグ インコーポレイテッド | 細胞学用培地に固定された組織サンプルから核酸またはタンパク質を回収するための組成物および方法 |
WO2011078439A1 (en) * | 2009-12-24 | 2011-06-30 | Seegene, Inc. | Real-time multiplexing detection of target nucleic acid sequences with elimination of false signals |
JP5876834B2 (ja) | 2010-01-04 | 2016-03-02 | キアジェン ゲイサーズバーグ インコーポレイテッド | 固定された組織サンプルからの核酸またはタンパク質の回収のための方法、組成物、およびキット |
EP2528932B1 (de) | 2010-01-29 | 2016-11-30 | QIAGEN Gaithersburg, Inc. | Verfahren und zusammensetzungen für sequenzspezifische reinigung und multiplex-analyse von nukleinsäuren |
EP2529031B1 (de) | 2010-01-29 | 2014-07-09 | QIAGEN Gaithersburg, Inc. | Verfahren zur feststellung von hpv in einer probe |
KR20160088958A (ko) | 2010-02-23 | 2016-07-26 | 루미넥스 코포레이션 | 일체화된 샘플 제조, 반응 및 검출을 위한 장치 및 방법 |
CA2799200A1 (en) * | 2010-05-19 | 2011-11-24 | Qiagen Gaithersburg, Inc. | Methods and compositions for sequence-specific purification and multiplex analysis of nucleic acids |
US9376727B2 (en) | 2010-05-25 | 2016-06-28 | Qiagen Gaithersburg, Inc. | Fast results hybrid capture assay and associated strategically truncated probes |
GB201020827D0 (en) * | 2010-12-08 | 2011-01-19 | Fotech Solutions Ltd | Distrubuted optical fibre sensor |
IT1403792B1 (it) * | 2010-12-30 | 2013-10-31 | St Microelectronics Srl | Analizzatore per analisi biochimiche e metodo per la determinazione di concentrazioni di sostanze fluorescenti in una soluzione |
US9361427B2 (en) * | 2011-02-01 | 2016-06-07 | The Regents Of The University Of California | Scar-less multi-part DNA assembly design automation |
EP2678664B1 (de) | 2011-02-24 | 2019-08-07 | Gen-Probe Incorporated | Systeme und verfahren zur unterscheidung optischer signale mit verschiedenen modulationsfrequenzen bei einem optischen signaldetektor |
JP2014511182A (ja) | 2011-02-24 | 2014-05-15 | キアゲン ガイサーズバーグ アイエヌシー. | Hpv核酸を検出するための材料及び方法 |
CN104023834B (zh) | 2011-05-04 | 2016-09-28 | 卢米耐克斯公司 | 用于集成的样品制备、反应和检测的设备与方法 |
JP2014515926A (ja) | 2011-05-12 | 2014-07-07 | ネットバイオ・インコーポレーテッド | Str遺伝子座の迅速な多重増幅のための方法および組成物 |
EP2525211B1 (de) | 2011-05-16 | 2018-01-03 | F. Hoffmann-La Roche AG | Instrument und verfahren zum detektieren von analyten |
JP5817369B2 (ja) * | 2011-09-13 | 2015-11-18 | ソニー株式会社 | スペクトル解析装置及び微小粒子測定装置、並びにスペクトル解析あるいはスペクトルチャート表示のための方法及びプログラム |
JP6103778B2 (ja) | 2011-11-03 | 2017-03-29 | キアジェン ゲイサーズバーグ インコーポレイテッド | カルボキシル化表面を使用して細胞を固定、単離、および濃縮するための材料および方法 |
EP3544015B1 (de) * | 2012-02-03 | 2024-10-23 | California Institute of Technology | Signalcodierung und -decodierung bei biochemischen multiplextests |
EP3901243A1 (de) | 2012-08-03 | 2021-10-27 | California Institute of Technology | Multiplexing und quantifizierung in pcr mit reduzierter hardware und reduzierten anforderungen |
US9932632B2 (en) | 2012-08-10 | 2018-04-03 | Streck, Inc. | Real-time optical system for polymerase chain reaction |
CN105008878B (zh) | 2012-12-05 | 2017-09-19 | 吉恩波克公司 | 光学探询器件 |
EP3014251A1 (de) | 2013-06-28 | 2016-05-04 | Streck Inc. | Vorrichtungen für echtzeit-polymerasekettenreaktionen |
US10718012B2 (en) | 2013-10-14 | 2020-07-21 | Agency For Science, Technology And Research | Non-motorized optical multiplexing for the simultaneous detection of DNA target amplicons in a polymerase chain reaction solution |
US9170198B2 (en) * | 2013-12-19 | 2015-10-27 | Luminex Corporation | Crosstalk reduction |
CN106457252B (zh) * | 2014-03-28 | 2020-07-14 | 好奇诊断有限责任公司 | 用于样本同时且均匀的热循环的装置及其用途 |
BE1022048B1 (nl) * | 2014-04-11 | 2016-02-10 | Ugentec Bvba | Methoden voor fluorescentie data correctie |
US9708647B2 (en) | 2015-03-23 | 2017-07-18 | Insilixa, Inc. | Multiplexed analysis of nucleic acid hybridization thermodynamics using integrated arrays |
US9499861B1 (en) | 2015-09-10 | 2016-11-22 | Insilixa, Inc. | Methods and systems for multiplex quantitative nucleic acid amplification |
WO2017155858A1 (en) | 2016-03-07 | 2017-09-14 | Insilixa, Inc. | Nucleic acid sequence identification using solid-phase cyclic single base extension |
WO2017218777A1 (en) | 2016-06-17 | 2017-12-21 | California Institute Of Technology | Nucleic acid reactions and related methods and compositions |
KR102264865B1 (ko) * | 2016-06-30 | 2021-06-14 | 주식회사 씨젠 | 반응 분석용 형광 검출 장치 |
KR102246285B1 (ko) * | 2017-03-07 | 2021-04-29 | 일루미나, 인코포레이티드 | 단일 광원, 2-광학 채널 서열분석 |
CA3176536C (en) | 2017-03-24 | 2024-03-05 | Gen-Probe Incorporated | Compositions and methods for detection of viral pathogens in samples |
CN111801340A (zh) | 2018-03-02 | 2020-10-20 | 生命技术公司 | 新型淬灭剂和报告基因染料组合 |
JP2022525322A (ja) | 2019-03-14 | 2022-05-12 | インシリクサ, インコーポレイテッド | 時間ゲート蛍光ベースの検出のための方法およびシステム |
US12054770B2 (en) | 2020-08-26 | 2024-08-06 | Electronics And Telecommunications Research Institute | Apparatus and method for PCR diagnosis based on multi-wavelength light source |
CN113308354B (zh) * | 2021-05-28 | 2022-02-15 | 深圳市尚维高科有限公司 | 一种多色荧光定量pcr仪光路系统及处理方法 |
EP4423502A1 (de) | 2021-10-26 | 2024-09-04 | Caribou Biosciences, Inc. | Exonuklease-gekoppelter echtzeit-endonukleaseaktivitätstest |
WO2024204236A1 (ja) * | 2023-03-27 | 2024-10-03 | 株式会社カネカ | 核酸増幅用反応液 |
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US5118801A (en) | 1988-09-30 | 1992-06-02 | The Public Health Research Institute | Nucleic acid process containing improved molecular switch |
US7081226B1 (en) * | 1996-06-04 | 2006-07-25 | University Of Utah Research Foundation | System and method for fluorescence monitoring |
US5750409A (en) | 1991-11-18 | 1998-05-12 | Boehringer Mannheim Gmbh | Pentacyclic compounds and their use as absorption or fluorescent dyes |
CA2129787A1 (en) * | 1993-08-27 | 1995-02-28 | Russell G. Higuchi | Monitoring multiple amplification reactions simultaneously and analyzing same |
US5538848A (en) | 1994-11-16 | 1996-07-23 | Applied Biosystems Division, Perkin-Elmer Corp. | Method for detecting nucleic acid amplification using self-quenching fluorescence probe |
US5654419A (en) * | 1994-02-01 | 1997-08-05 | The Regents Of The University Of California | Fluorescent labels and their use in separations |
US5869255A (en) * | 1994-02-01 | 1999-02-09 | The Regents Of The University Of California | Probes labeled with energy transfer couples dyes exemplified with DNA fragment analysis |
EP0706649B1 (de) | 1994-04-29 | 2001-01-03 | Perkin-Elmer Corporation | Verfahren und vorrichtung zur echtzeiterfassung der produkte von nukleinsäureamplifikation |
US5563588A (en) * | 1994-08-02 | 1996-10-08 | Belfer; Bruce D. | Fiber optic traffic signal light system having a shutter control |
WO1996037767A1 (en) * | 1995-05-26 | 1996-11-28 | Optical Analytic Inc. | Wide angle scattering detector |
DE19521231A1 (de) | 1995-06-10 | 1996-12-12 | Boehringer Mannheim Gmbh | Neue Oxazinfarbstoffe und ihre Verwendung als Fluoreszenzmarker |
US5837196A (en) * | 1996-01-26 | 1998-11-17 | The Regents Of The University Of California | High density array fabrication and readout method for a fiber optic biosensor |
ES2260562T3 (es) * | 1996-06-04 | 2006-11-01 | University Of Utah Research Foundation | Sistema y metodo para llevar a cabo y supervisar procesos biologicos. |
AU726501B2 (en) | 1996-06-04 | 2000-11-09 | University Of Utah Research Foundation | Monitoring hybridization during PCR |
US5853992A (en) * | 1996-10-04 | 1998-12-29 | The Regents Of The University Of California | Cyanine dyes with high-absorbance cross section as donor chromophores in energy transfer labels |
US6027709A (en) | 1997-01-10 | 2000-02-22 | Li-Cor Inc. | Fluorescent cyanine dyes |
MA24525A1 (fr) | 1997-04-14 | 1998-12-31 | Procter & Gamble | Particule detergente |
AUPO652997A0 (en) * | 1997-04-30 | 1997-05-29 | Kindconi Pty Limited | Temperature cycling device and method |
US6369893B1 (en) * | 1998-05-19 | 2002-04-09 | Cepheid | Multi-channel optical detection system |
AU746069B2 (en) * | 1998-05-19 | 2002-04-11 | Cepheid | Multi-channel optical detection system |
US6140054A (en) | 1998-09-30 | 2000-10-31 | University Of Utah Research Foundation | Multiplex genotyping using fluorescent hybridization probes |
US6197520B1 (en) | 1999-08-13 | 2001-03-06 | University Of Utah Research Foundation | Solution-based color compensation adjusted for temperature and electronic gains |
WO2002014555A2 (en) | 2000-08-11 | 2002-02-21 | University Of Utah Research Foundation | Single-labeled oligonucleotide probes |
US6870165B2 (en) * | 2001-10-19 | 2005-03-22 | Biocal Technology, Inc. | Multi-color multiplexed analysis in a bio-separation system |
US7635588B2 (en) * | 2001-11-29 | 2009-12-22 | Applied Biosystems, Llc | Apparatus and method for differentiating multiple fluorescence signals by excitation wavelength |
DE60333480D1 (de) * | 2002-12-06 | 2010-09-02 | Roche Diagnostics Gmbh | Verfahren zum nachweis von pathogenen organismen |
JP2006521092A (ja) | 2003-04-04 | 2006-09-21 | エフ.ホフマン−ラ ロシュ アーゲー | マルチカラーリアルタイムpcr用の改良されたシステム |
-
2004
- 2004-04-01 JP JP2005518687A patent/JP2006521092A/ja not_active Withdrawn
- 2004-04-01 AT AT04725021T patent/ATE550099T1/de active
- 2004-04-01 EP EP04725021A patent/EP1613771B1/de not_active Expired - Lifetime
- 2004-04-01 CA CA2525482A patent/CA2525482C/en not_active Expired - Lifetime
- 2004-04-01 EP EP10170020A patent/EP2284278A1/de not_active Withdrawn
- 2004-04-01 WO PCT/EP2004/003457 patent/WO2004087950A2/en active Application Filing
- 2004-04-01 ES ES04725021T patent/ES2384170T3/es not_active Expired - Lifetime
- 2004-04-01 US US10/549,648 patent/US8137616B2/en not_active Expired - Lifetime
-
2009
- 2009-07-21 JP JP2009170421A patent/JP5144605B2/ja not_active Expired - Lifetime
-
2011
- 2011-02-08 US US13/023,407 patent/US20110151550A1/en not_active Abandoned
Also Published As
Publication number | Publication date |
---|---|
EP1613771B1 (de) | 2012-03-21 |
WO2004087950A3 (en) | 2004-11-25 |
US8137616B2 (en) | 2012-03-20 |
WO2004087950A2 (en) | 2004-10-14 |
CA2525482A1 (en) | 2004-10-14 |
JP2009232871A (ja) | 2009-10-15 |
ES2384170T3 (es) | 2012-07-02 |
EP1613771A2 (de) | 2006-01-11 |
JP5144605B2 (ja) | 2013-02-13 |
CA2525482C (en) | 2015-02-17 |
US20060269922A1 (en) | 2006-11-30 |
JP2006521092A (ja) | 2006-09-21 |
EP2284278A1 (de) | 2011-02-16 |
US20110151550A1 (en) | 2011-06-23 |
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