JP4480715B2 - 二重末端シーケンシング - Google Patents

二重末端シーケンシング Download PDF

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JP4480715B2
JP4480715B2 JP2006503134A JP2006503134A JP4480715B2 JP 4480715 B2 JP4480715 B2 JP 4480715B2 JP 2006503134 A JP2006503134 A JP 2006503134A JP 2006503134 A JP2006503134 A JP 2006503134A JP 4480715 B2 JP4480715 B2 JP 4480715B2
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primer
sequencing
nucleic acid
polymerase
primers
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JP2006515522A (ja
JP2006515522A5 (https=
Inventor
イ−ジュ チェン
ジョン エイチ． リーモン
ケントン ローマン
マイケル ティー． ロナン
メイスレイアン スリニバサン
ジョナサン ロスバーグ
マイケル ウェイナー
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４５４ コーポレーション
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    • C—CHEMISTRY; METALLURGY
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
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    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502707—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the manufacture of the container or its components
    • B—PERFORMING OPERATIONS; TRANSPORTING
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    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502715—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
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    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C—CHEMISTRY; METALLURGY
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    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1075—Isolating an individual clone by screening libraries by coupling phenotype to genotype, not provided for in other groups of this subclass
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    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
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    • C12Q1/6865—Promoter-based amplification, e.g. nucleic acid sequence amplification [NASBA], self-sustained sequence replication [3SR] or transcription-based amplification system [TAS]
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    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6867—Replicase-based amplification, e.g. using Q-beta replicase
    • C—CHEMISTRY; METALLURGY
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    • C12Q1/6869—Methods for sequencing
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    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/14—Heterocyclic carbon compound [i.e., O, S, N, Se, Te, as only ring hetero atom]
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  • Chemical & Material Sciences (AREA)
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  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Genetics & Genomics (AREA)
  • Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biomedical Technology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Clinical Laboratory Science (AREA)
  • Dispersion Chemistry (AREA)
  • Hematology (AREA)
  • Plant Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Optics & Photonics (AREA)
  • Condensed Matter Physics & Semiconductors (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Saccharide Compounds (AREA)
  • Colloid Chemistry (AREA)
  • Emulsifying, Dispersing, Foam-Producing Or Wetting Agents (AREA)
JP2006503134A 2003-01-29 2004-01-28 二重末端シーケンシング Expired - Fee Related JP4480715B2 (ja)

Applications Claiming Priority (8)

Application Number Priority Date Filing Date Title
US44347103P 2003-01-29 2003-01-29
US46507103P 2003-04-23 2003-04-23
US47660203P 2003-06-06 2003-06-06
US47650403P 2003-06-06 2003-06-06
US47631303P 2003-06-06 2003-06-06
US47659203P 2003-06-06 2003-06-06
US49798503P 2003-08-25 2003-08-25
PCT/US2004/002485 WO2004070005A2 (en) 2003-01-29 2004-01-28 Double ended sequencing

Publications (3)

Publication Number Publication Date
JP2006515522A JP2006515522A (ja) 2006-06-01
JP2006515522A5 JP2006515522A5 (https=) 2010-04-08
JP4480715B2 true JP4480715B2 (ja) 2010-06-16

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JP2006503134A Expired - Fee Related JP4480715B2 (ja) 2003-01-29 2004-01-28 二重末端シーケンシング
JP2006532273A Expired - Lifetime JP4473878B2 (ja) 2003-01-29 2004-01-28 核酸を増幅および配列決定する方法
JP2006503163A Pending JP2007525151A (ja) 2003-01-29 2004-01-28 一本鎖ｄｎａライブラリーの調製方法
JP2006503133A Expired - Lifetime JP4768598B2 (ja) 2003-01-29 2004-01-28 ビーズエマルジョン核酸増幅
JP2009297745A Pending JP2010142233A (ja) 2003-01-29 2009-12-28 核酸を増幅および配列決定する方法
JP2010053827A Expired - Lifetime JP5258822B2 (ja) 2003-01-29 2010-03-10 ビーズエマルジョン核酸増幅
JP2012181295A Expired - Lifetime JP5495399B2 (ja) 2003-01-29 2012-08-20 核酸を増幅および配列決定する方法
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