ATE506449T1 - Direkte rt-pcr auf oligonukleotid-immobilisierten pcr-mikroplatten - Google Patents
Direkte rt-pcr auf oligonukleotid-immobilisierten pcr-mikroplattenInfo
- Publication number
- ATE506449T1 ATE506449T1 AT07016364T AT07016364T ATE506449T1 AT E506449 T1 ATE506449 T1 AT E506449T1 AT 07016364 T AT07016364 T AT 07016364T AT 07016364 T AT07016364 T AT 07016364T AT E506449 T1 ATE506449 T1 AT E506449T1
- Authority
- AT
- Austria
- Prior art keywords
- pcr
- microplates
- immobilized
- oligonucleotide
- filter plate
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/06—Lysis of microorganisms
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
- C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6851—Quantitative amplification
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biochemistry (AREA)
- Analytical Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- General Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Physics & Mathematics (AREA)
- Immunology (AREA)
- Biophysics (AREA)
- Tropical Medicine & Parasitology (AREA)
- Medicinal Chemistry (AREA)
- Mycology (AREA)
- Virology (AREA)
- Biomedical Technology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Saccharide Compounds (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US6839497P | 1997-12-22 | 1997-12-22 | |
US7162798P | 1998-01-16 | 1998-01-16 |
Publications (1)
Publication Number | Publication Date |
---|---|
ATE506449T1 true ATE506449T1 (de) | 2011-05-15 |
Family
ID=26748929
Family Applications (3)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
AT07016364T ATE506449T1 (de) | 1997-12-22 | 1998-12-22 | Direkte rt-pcr auf oligonukleotid-immobilisierten pcr-mikroplatten |
AT98964860T ATE401415T1 (de) | 1997-12-22 | 1998-12-22 | Direkte rt-pcr auf mikrotiterplatten mit immobilisierten oligonukleotid |
AT05017491T ATE397086T1 (de) | 1997-12-22 | 1998-12-22 | Reinigung von mrna auf mikroplatten |
Family Applications After (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
AT98964860T ATE401415T1 (de) | 1997-12-22 | 1998-12-22 | Direkte rt-pcr auf mikrotiterplatten mit immobilisierten oligonukleotid |
AT05017491T ATE397086T1 (de) | 1997-12-22 | 1998-12-22 | Reinigung von mrna auf mikroplatten |
Country Status (5)
Country | Link |
---|---|
EP (3) | EP1042497B1 (de) |
JP (3) | JP4325114B2 (de) |
AT (3) | ATE506449T1 (de) |
DE (3) | DE69839734D1 (de) |
WO (1) | WO1999032654A1 (de) |
Families Citing this family (56)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6844158B1 (en) | 1997-12-22 | 2005-01-18 | Hitachi Chemical Co., Ltd. | Direct RT-PCR on oligonucleotide-immobilized PCR microplates |
US7399589B2 (en) | 1998-02-06 | 2008-07-15 | Digene Corporation | Immunological detection of RNA:DNA hybrids on microarrays |
US6686151B1 (en) | 1998-02-06 | 2004-02-03 | Digene Corporation | Immunological detection of RNA:DNA hybrids on microarrays |
US20050100943A1 (en) | 2000-04-11 | 2005-05-12 | Hideki Kambara | Method of producing probe arrays for biological materials using fine particles |
CN1245520C (zh) * | 1999-04-12 | 2006-03-15 | 日立化成工业株式会社 | 使用精细颗粒生产生物材料的探针列阵的方法 |
FR2798675B1 (fr) * | 1999-09-16 | 2004-02-27 | Centre Nat Rech Scient | Procede et dispositif de fabrication d'un support porteur d'une pluralite de sequences polynucleotidiques et/ou peptidiques differentes |
CA2318730C (en) * | 1999-09-20 | 2008-01-22 | Ortho-Clinical Diagnostics, Inc. | A method for reverse transcribing rna isolated in dry form |
US6642000B1 (en) * | 1999-11-12 | 2003-11-04 | University Of Chicago | PCR amplification on microarrays of gel immobilized oligonucleotides |
ITCA20000004A1 (it) * | 2000-03-07 | 2001-09-07 | Mario Raimondo Caria | Metodo e sistema per la rivelazione simultanea e multipla con quantificazione della ibridizzazione di composti molecolari quali acidi nuclei |
US6376191B1 (en) * | 2000-03-22 | 2002-04-23 | Mergen, Ltd. | Microarray-based analysis of polynucleotide sequence variations |
US7439016B1 (en) | 2000-06-15 | 2008-10-21 | Digene Corporation | Detection of nucleic acids by type-specific hybrid capture method |
US7601497B2 (en) | 2000-06-15 | 2009-10-13 | Qiagen Gaithersburg, Inc. | Detection of nucleic acids by target-specific hybrid capture method |
DE10033091A1 (de) * | 2000-07-07 | 2002-01-31 | Max Planck Gesellschaft | Polymer-Chip |
AU2000275323A1 (en) * | 2000-09-27 | 2002-04-08 | Centre National De La Recherche Scientifique (C.N.R.S.) | Method and device for making a support bearing a plurality of different polynucleotide and/or peptide sequences |
US7285199B2 (en) | 2000-10-31 | 2007-10-23 | Hitachi Chemical Research Center, Inc. | Apparatus and method for electrophoretic microspot concentration |
WO2002066637A2 (en) * | 2000-10-31 | 2002-08-29 | Hitachi Chemical Research Center, Inc. | Method for collecting and using nuclear mrna |
GB0105790D0 (en) * | 2001-03-08 | 2001-04-25 | Expresson Biosystems Ltd | Detecting binding of mRNA to an oligonnucleotide array using RNA dependent nucleic acid modifying enzymes |
CN100487116C (zh) * | 2001-07-09 | 2009-05-13 | 旭化成株式会社 | 应用非织造织物纯化和检测核酸的方法 |
JP4471659B2 (ja) * | 2002-01-09 | 2010-06-02 | シスメックス株式会社 | 直接核酸増幅法 |
EP1504121A4 (de) * | 2002-04-24 | 2005-06-29 | Hitachi Chemical Res Ct Inc | Vorrichtung und verfahren zur quantifizierung von mrna aus vollblut mit hohem durchsatz |
US7745180B2 (en) | 2002-04-24 | 2010-06-29 | Hitachi Chemical Co., Ltd. | Device and method for high-throughput quantification of mRNA from whole blood |
US20040067492A1 (en) * | 2002-10-04 | 2004-04-08 | Allan Peng | Reverse transcription on microarrays |
DE602004026033D1 (de) | 2003-01-29 | 2010-04-29 | 454 Corp | Zweiendige sequenzierung |
CN1580283A (zh) * | 2003-08-13 | 2005-02-16 | 清华大学 | 一种检测核酸分子的方法 |
DE10353887A1 (de) * | 2003-11-18 | 2005-06-16 | Febit Ag | Hochparalleler DNA-Synthesizer auf Matrizenbasis |
EP1548126A1 (de) * | 2003-12-22 | 2005-06-29 | Bio-Rad Pasteur | Trägermaterial für Kontrollnukleinsäuren und Anwendung dieser zur Nukleinsäuredetektion. |
US20050277121A1 (en) * | 2004-06-11 | 2005-12-15 | Ambion, Inc. | Crude biological derivatives competent for nucleic acid detection |
EP2194485B1 (de) | 2004-11-16 | 2012-10-17 | Illumina, Inc. | Verfahren und Vorrichtung zum Auslesen kodierter Mikroperlen |
WO2006088601A2 (en) * | 2005-01-21 | 2006-08-24 | New York Blood Center, Inc., The | Method for extraction and identification of nucleic acids |
WO2006116708A2 (en) * | 2005-04-28 | 2006-11-02 | Hitachi Chemical Research Center, Inc. | Method of minimizing reagent consumption in microplate-based reactions |
FR2885140B1 (fr) * | 2005-04-29 | 2010-12-31 | Millipore Corp | Procede de detection et de caracterisation de microorgarnismes sur une membrane. |
JP4916689B2 (ja) * | 2005-09-12 | 2012-04-18 | 住友ベークライト株式会社 | Dna鎖増幅方法 |
US7830575B2 (en) | 2006-04-10 | 2010-11-09 | Illumina, Inc. | Optical scanner with improved scan time |
JPWO2007141912A1 (ja) * | 2006-06-07 | 2009-10-15 | 住友ベークライト株式会社 | Rna検出方法 |
US7840065B2 (en) | 2006-07-12 | 2010-11-23 | Color Savvy Systems Limited | Method for deriving consistent color measurements |
CN101558167A (zh) * | 2006-08-01 | 2009-10-14 | 应用生物系统有限公司 | 分析物和核酸的检测 |
EP2347011B1 (de) | 2008-10-27 | 2017-01-18 | QIAGEN Gaithersburg, Inc. | Rna/dna hybrid detektionsverfahren |
EP2425019B1 (de) | 2009-05-01 | 2014-03-19 | QIAGEN Gaithersburg, Inc. | Ungezieltes verstärkungsverfahren zur erkennung von rna-spleissformen in einer probe |
JP5729904B2 (ja) | 2009-06-02 | 2015-06-03 | キヤノン株式会社 | 細胞から蛋白質、dna、rnaを調製する方法 |
EP2454378B1 (de) * | 2009-07-17 | 2015-12-23 | Canon U.S. Life Sciences, Inc. | Verfahren und systeme für dna-isolation bei einer mikrofluidischen vorrichtung |
AU2010291990B2 (en) | 2009-09-14 | 2016-05-05 | Qiagen Gaithersburg, Inc. | Compositions and methods for recovery of nucleic acids or proteins from tissue samples fixed in cytology media |
JP6103941B2 (ja) | 2010-01-29 | 2017-03-29 | キアジェン ゲイサーズバーグ インコーポレイテッド | 核酸の配列特異的精製および多重分析のための方法および組成物 |
EP2529031B1 (de) | 2010-01-29 | 2014-07-09 | QIAGEN Gaithersburg, Inc. | Verfahren zur feststellung von hpv in einer probe |
CN102834526B (zh) | 2010-04-05 | 2015-12-02 | 普罗格诺西斯生物科学公司 | 空间编码的生物学测定 |
US20190300945A1 (en) | 2010-04-05 | 2019-10-03 | Prognosys Biosciences, Inc. | Spatially Encoded Biological Assays |
US10787701B2 (en) | 2010-04-05 | 2020-09-29 | Prognosys Biosciences, Inc. | Spatially encoded biological assays |
AU2011255638B2 (en) | 2010-05-19 | 2016-08-25 | Qiagen Gaithersburg, Inc. | Methods and compositions for sequence-specific purification and multiplex analysis of nucleic acids |
US9376727B2 (en) | 2010-05-25 | 2016-06-28 | Qiagen Gaithersburg, Inc. | Fast results hybrid capture assay and associated strategically truncated probes |
AU2012220575B2 (en) | 2011-02-24 | 2017-01-12 | Qiagen Gaithersburg, Inc. | Materials and methods for detection of HPV nucleic acid |
GB201106254D0 (en) | 2011-04-13 | 2011-05-25 | Frisen Jonas | Method and product |
EP2766493B1 (de) * | 2011-10-11 | 2019-12-18 | Qiagen GmbH | Probenverarbeitungsverfahren und probenverarbeitungskartusche |
US9593365B2 (en) | 2012-10-17 | 2017-03-14 | Spatial Transcriptions Ab | Methods and product for optimising localised or spatial detection of gene expression in a tissue sample |
JP2014176303A (ja) * | 2013-03-13 | 2014-09-25 | Seiko Epson Corp | cDNAの合成方法 |
CN105849275B (zh) | 2013-06-25 | 2020-03-17 | 普罗格诺西斯生物科学公司 | 检测样品中生物靶标的空间分布的方法和系统 |
EP4119677B1 (de) | 2015-04-10 | 2023-06-28 | Spatial Transcriptomics AB | Räumlich getrennte multiplex-nukleinsäureanalyse von biologischen proben |
CN106867993A (zh) * | 2017-02-17 | 2017-06-20 | 唐旌生物科技(上海)有限公司 | 一种逆转录方法、逆转录试剂盒及其应用 |
Family Cites Families (11)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE4034036C2 (de) * | 1990-10-26 | 1994-03-03 | Diagen Inst Molekularbio | Vorrichtung und Verfahren zur Isolierung von Nukleinsäuren aus Zellsuspensionen |
JPH04187077A (ja) * | 1990-11-22 | 1992-07-03 | Shimadzu Corp | 核酸の抽出精製装置 |
JPH04360686A (ja) * | 1991-06-04 | 1992-12-14 | Tosoh Corp | Dnaの精製方法 |
US5437976A (en) * | 1991-08-08 | 1995-08-01 | Arizona Board Of Regents, The University Of Arizona | Multi-domain DNA ligands bound to a solid matrix for protein and nucleic acid affinity chromatography and processing of solid-phase DNA |
CA2122450C (en) * | 1991-11-01 | 2004-07-13 | Charles Phillip Morris | Solid phase amplification process |
JPH08500722A (ja) * | 1992-01-29 | 1996-01-30 | 日立化成工業株式会社 | ポリヌクレオチド固定化担体 |
US5545528A (en) * | 1993-04-15 | 1996-08-13 | Hitachi Chemical Research Center | Rapid screening method of gene amplification products in polypropylene plates |
GB9314249D0 (en) * | 1993-07-09 | 1993-08-18 | Proofname Ltd | Purification method and apparatus |
GB9507238D0 (en) * | 1995-04-07 | 1995-05-31 | Isis Innovation | Detecting dna sequence variations |
CA2223896A1 (en) * | 1995-06-08 | 1996-12-27 | Robert Hugh Don | Method and apparatus for dna extraction |
DE69719409T2 (de) * | 1996-06-18 | 2004-01-15 | Rikagaku Kenkyusho | Verfahren zur Reinigung von DNS |
-
1998
- 1998-12-22 AT AT07016364T patent/ATE506449T1/de not_active IP Right Cessation
- 1998-12-22 DE DE69839734T patent/DE69839734D1/de not_active Expired - Lifetime
- 1998-12-22 EP EP98964860A patent/EP1042497B1/de not_active Expired - Lifetime
- 1998-12-22 WO PCT/US1998/027293 patent/WO1999032654A1/en active Application Filing
- 1998-12-22 DE DE69839572T patent/DE69839572D1/de not_active Expired - Lifetime
- 1998-12-22 EP EP07016364A patent/EP1865074B1/de not_active Expired - Lifetime
- 1998-12-22 DE DE69842238T patent/DE69842238D1/de not_active Expired - Lifetime
- 1998-12-22 AT AT98964860T patent/ATE401415T1/de not_active IP Right Cessation
- 1998-12-22 AT AT05017491T patent/ATE397086T1/de not_active IP Right Cessation
- 1998-12-22 JP JP2000525571A patent/JP4325114B2/ja not_active Expired - Lifetime
- 1998-12-22 EP EP05017491A patent/EP1600512B1/de not_active Expired - Lifetime
-
2005
- 2005-09-26 JP JP2005278646A patent/JP4337796B2/ja not_active Expired - Lifetime
-
2009
- 2009-04-13 JP JP2009097473A patent/JP4983836B2/ja not_active Expired - Lifetime
Also Published As
Publication number | Publication date |
---|---|
DE69839734D1 (de) | 2008-08-28 |
EP1042497A4 (de) | 2004-09-22 |
JP2006051042A (ja) | 2006-02-23 |
EP1865074A3 (de) | 2008-02-27 |
ATE397086T1 (de) | 2008-06-15 |
JP2009189368A (ja) | 2009-08-27 |
EP1600512A1 (de) | 2005-11-30 |
WO1999032654A1 (en) | 1999-07-01 |
EP1865074B1 (de) | 2011-04-20 |
DE69839572D1 (de) | 2008-07-10 |
DE69842238D1 (de) | 2011-06-01 |
EP1600512B1 (de) | 2008-05-28 |
JP4325114B2 (ja) | 2009-09-02 |
EP1042497B1 (de) | 2008-07-16 |
JP4983836B2 (ja) | 2012-07-25 |
JP2002505080A (ja) | 2002-02-19 |
EP1042497A1 (de) | 2000-10-11 |
JP4337796B2 (ja) | 2009-09-30 |
ATE401415T1 (de) | 2008-08-15 |
EP1865074A2 (de) | 2007-12-12 |
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Legal Events
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RER | Ceased as to paragraph 5 lit. 3 law introducing patent treaties |