ATE401415T1 - Direkte rt-pcr auf mikrotiterplatten mit immobilisierten oligonukleotid - Google Patents

Direkte rt-pcr auf mikrotiterplatten mit immobilisierten oligonukleotid

Info

Publication number
ATE401415T1
ATE401415T1 AT98964860T AT98964860T ATE401415T1 AT E401415 T1 ATE401415 T1 AT E401415T1 AT 98964860 T AT98964860 T AT 98964860T AT 98964860 T AT98964860 T AT 98964860T AT E401415 T1 ATE401415 T1 AT E401415T1
Authority
AT
Austria
Prior art keywords
pcr
microplates
immobilized
direct
filter plate
Prior art date
Application number
AT98964860T
Other languages
English (en)
Inventor
Masato Mitsuhashi
Original Assignee
Hitachi Chemical Co Ltd
Hitachi Chemical Res Ct Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hitachi Chemical Co Ltd, Hitachi Chemical Res Ct Inc filed Critical Hitachi Chemical Co Ltd
Application granted granted Critical
Publication of ATE401415T1 publication Critical patent/ATE401415T1/de

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/686Polymerase chain reaction [PCR]
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/06Lysis of microorganisms
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • C12Q1/6834Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C12Q1/6837Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/6851Quantitative amplification

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Biophysics (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Medicinal Chemistry (AREA)
  • Mycology (AREA)
  • Virology (AREA)
  • Biomedical Technology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
AT98964860T 1997-12-22 1998-12-22 Direkte rt-pcr auf mikrotiterplatten mit immobilisierten oligonukleotid ATE401415T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US6839497P 1997-12-22 1997-12-22
US7162798P 1998-01-16 1998-01-16

Publications (1)

Publication Number Publication Date
ATE401415T1 true ATE401415T1 (de) 2008-08-15

Family

ID=26748929

Family Applications (3)

Application Number Title Priority Date Filing Date
AT05017491T ATE397086T1 (de) 1997-12-22 1998-12-22 Reinigung von mrna auf mikroplatten
AT98964860T ATE401415T1 (de) 1997-12-22 1998-12-22 Direkte rt-pcr auf mikrotiterplatten mit immobilisierten oligonukleotid
AT07016364T ATE506449T1 (de) 1997-12-22 1998-12-22 Direkte rt-pcr auf oligonukleotid-immobilisierten pcr-mikroplatten

Family Applications Before (1)

Application Number Title Priority Date Filing Date
AT05017491T ATE397086T1 (de) 1997-12-22 1998-12-22 Reinigung von mrna auf mikroplatten

Family Applications After (1)

Application Number Title Priority Date Filing Date
AT07016364T ATE506449T1 (de) 1997-12-22 1998-12-22 Direkte rt-pcr auf oligonukleotid-immobilisierten pcr-mikroplatten

Country Status (5)

Country Link
EP (3) EP1865074B1 (de)
JP (3) JP4325114B2 (de)
AT (3) ATE397086T1 (de)
DE (3) DE69842238D1 (de)
WO (1) WO1999032654A1 (de)

Families Citing this family (69)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6844158B1 (en) 1997-12-22 2005-01-18 Hitachi Chemical Co., Ltd. Direct RT-PCR on oligonucleotide-immobilized PCR microplates
US7399589B2 (en) 1998-02-06 2008-07-15 Digene Corporation Immunological detection of RNA:DNA hybrids on microarrays
US6686151B1 (en) * 1998-02-06 2004-02-03 Digene Corporation Immunological detection of RNA:DNA hybrids on microarrays
CN1245520C (zh) * 1999-04-12 2006-03-15 日立化成工业株式会社 使用精细颗粒生产生物材料的探针列阵的方法
US20050100943A1 (en) 2000-04-11 2005-05-12 Hideki Kambara Method of producing probe arrays for biological materials using fine particles
FR2798675B1 (fr) * 1999-09-16 2004-02-27 Centre Nat Rech Scient Procede et dispositif de fabrication d'un support porteur d'une pluralite de sequences polynucleotidiques et/ou peptidiques differentes
CA2318730C (en) * 1999-09-20 2008-01-22 Ortho-Clinical Diagnostics, Inc. A method for reverse transcribing rna isolated in dry form
US6642000B1 (en) * 1999-11-12 2003-11-04 University Of Chicago PCR amplification on microarrays of gel immobilized oligonucleotides
ITCA20000004A1 (it) * 2000-03-07 2001-09-07 Mario Raimondo Caria Metodo e sistema per la rivelazione simultanea e multipla con quantificazione della ibridizzazione di composti molecolari quali acidi nuclei
US6376191B1 (en) * 2000-03-22 2002-04-23 Mergen, Ltd. Microarray-based analysis of polynucleotide sequence variations
US7601497B2 (en) 2000-06-15 2009-10-13 Qiagen Gaithersburg, Inc. Detection of nucleic acids by target-specific hybrid capture method
US7439016B1 (en) 2000-06-15 2008-10-21 Digene Corporation Detection of nucleic acids by type-specific hybrid capture method
DE10033091A1 (de) * 2000-07-07 2002-01-31 Max Planck Gesellschaft Polymer-Chip
AU2000275323A1 (en) * 2000-09-27 2002-04-08 Centre National De La Recherche Scientifique (C.N.R.S.) Method and device for making a support bearing a plurality of different polynucleotide and/or peptide sequences
WO2002066637A2 (en) * 2000-10-31 2002-08-29 Hitachi Chemical Research Center, Inc. Method for collecting and using nuclear mrna
WO2002042500A2 (en) 2000-10-31 2002-05-30 Hitachi Chemical Research Center, Inc. Apparatus and method for electrophoretic microspot concentration
GB0105790D0 (en) * 2001-03-08 2001-04-25 Expresson Biosystems Ltd Detecting binding of mRNA to an oligonnucleotide array using RNA dependent nucleic acid modifying enzymes
WO2003006650A1 (en) * 2001-07-09 2003-01-23 Asahi Kasei Kabushiki Kaisha Method of purifying nucleic acid using nonwoven fabric and detection method
ATE485372T1 (de) 2002-01-09 2010-11-15 Sysmex Corp Nukleinsäurenachweisverfahren und system dafür
CN1650027B (zh) * 2002-04-24 2010-04-21 日立化成研究中心公司 用于全血mRNA的高通量定量的装置和方法
US7745180B2 (en) 2002-04-24 2010-06-29 Hitachi Chemical Co., Ltd. Device and method for high-throughput quantification of mRNA from whole blood
US7508608B2 (en) 2004-11-17 2009-03-24 Illumina, Inc. Lithographically fabricated holographic optical identification element
US7441703B2 (en) 2002-08-20 2008-10-28 Illumina, Inc. Optical reader for diffraction grating-based encoded optical identification elements
CA2499046A1 (en) 2002-09-12 2004-03-25 Cyvera Corporation Diffraction grating-based encoded micro-particles for multiplexed experiments
CA2498933C (en) 2002-09-12 2012-08-28 Cyvera Corporation Method and apparatus for aligning elongated microbeads in order to interrogate the same
US7092160B2 (en) 2002-09-12 2006-08-15 Illumina, Inc. Method of manufacturing of diffraction grating-based optical identification element
US20040067492A1 (en) * 2002-10-04 2004-04-08 Allan Peng Reverse transcription on microarrays
CN101128601B (zh) 2003-01-29 2011-06-08 454生命科学公司 核酸扩增和测序的方法
CN1580283A (zh) * 2003-08-13 2005-02-16 清华大学 一种检测核酸分子的方法
DE10353887A1 (de) * 2003-11-18 2005-06-16 Febit Ag Hochparalleler DNA-Synthesizer auf Matrizenbasis
EP1548126A1 (de) * 2003-12-22 2005-06-29 Bio-Rad Pasteur Trägermaterial für Kontrollnukleinsäuren und Anwendung dieser zur Nukleinsäuredetektion.
US7433123B2 (en) 2004-02-19 2008-10-07 Illumina, Inc. Optical identification element having non-waveguide photosensitive substrate with diffraction grating therein
US20050277121A1 (en) * 2004-06-11 2005-12-15 Ambion, Inc. Crude biological derivatives competent for nucleic acid detection
WO2006020363A2 (en) 2004-07-21 2006-02-23 Illumina, Inc. Method and apparatus for drug product tracking using encoded optical identification elements
CN101116086A (zh) 2004-11-16 2008-01-30 伊路敏纳公司 读取编码微珠的方法和设备
US7602952B2 (en) 2004-11-16 2009-10-13 Illumina, Inc. Scanner having spatial light modulator
US7604173B2 (en) 2004-11-16 2009-10-20 Illumina, Inc. Holographically encoded elements for microarray and other tagging labeling applications, and method and apparatus for making and reading the same
CA2594491A1 (en) * 2005-01-21 2006-08-24 The New York Blood Center, Inc. Method for extraction and identification of nucleic acids
EP1875243B1 (de) * 2005-04-28 2013-04-03 Hitachi Chemical Research Center, Inc. Verfahren zur minimierung des reagenzverbrauchs bei auf mikroplatten basierenden reaktionen
FR2885140B1 (fr) * 2005-04-29 2010-12-31 Millipore Corp Procede de detection et de caracterisation de microorgarnismes sur une membrane.
JP4916689B2 (ja) * 2005-09-12 2012-04-18 住友ベークライト株式会社 Dna鎖増幅方法
US7623624B2 (en) 2005-11-22 2009-11-24 Illumina, Inc. Method and apparatus for labeling using optical identification elements characterized by X-ray diffraction
US7830575B2 (en) 2006-04-10 2010-11-09 Illumina, Inc. Optical scanner with improved scan time
JPWO2007141912A1 (ja) * 2006-06-07 2009-10-15 住友ベークライト株式会社 Rna検出方法
WO2008008878A2 (en) 2006-07-12 2008-01-17 Color Savvy Systems Limited Method for deriving consistent color measurements
BRPI0715346A2 (pt) * 2006-08-01 2013-11-26 Applera Corp Detecção de analitos e ácidos nucléicos
ES2622062T3 (es) 2008-10-27 2017-07-05 Qiagen Gaithersburg, Inc. Ensayo y sistema de captura de híbrido de resultados rápidos
US9797000B2 (en) 2009-05-01 2017-10-24 Qiagen Gaithersburg Inc. Non-target amplification method for detection of RNA splice-forms in a sample
JP5729904B2 (ja) * 2009-06-02 2015-06-03 キヤノン株式会社 細胞から蛋白質、dna、rnaを調製する方法
JP5815518B2 (ja) * 2009-07-17 2015-11-17 キヤノン ユー.エス. ライフ サイエンシズ, インコーポレイテッドCanon U.S. Life Sciences, Inc. マイクロ流体装置においてdnaを単離する方法及びシステム
JP5826752B2 (ja) 2009-09-14 2015-12-02 キアジェン ゲイサーズバーグ インコーポレイテッド 細胞学用培地に固定された組織サンプルから核酸またはタンパク質を回収するための組成物および方法
AU2011210748A1 (en) 2010-01-29 2012-08-09 Qiagen Gaithersburg, Inc. Method of determining and confirming the presence of HPV in a sample
JP6103941B2 (ja) 2010-01-29 2017-03-29 キアジェン ゲイサーズバーグ インコーポレイテッド 核酸の配列特異的精製および多重分析のための方法および組成物
US10787701B2 (en) 2010-04-05 2020-09-29 Prognosys Biosciences, Inc. Spatially encoded biological assays
US20190300945A1 (en) 2010-04-05 2019-10-03 Prognosys Biosciences, Inc. Spatially Encoded Biological Assays
ES2555106T3 (es) 2010-04-05 2015-12-29 Prognosys Biosciences, Inc. Ensayos biológicos codificados espacialmente
AU2011255638B2 (en) 2010-05-19 2016-08-25 Qiagen Gaithersburg, Inc. Methods and compositions for sequence-specific purification and multiplex analysis of nucleic acids
US9376727B2 (en) 2010-05-25 2016-06-28 Qiagen Gaithersburg, Inc. Fast results hybrid capture assay and associated strategically truncated probes
EP2678442B1 (de) 2011-02-24 2019-01-16 QIAGEN Gaithersburg, Inc. Materialien und verfahren für den nachweis von hpv-nukleinsäuren
GB201106254D0 (en) 2011-04-13 2011-05-25 Frisen Jonas Method and product
US9994891B2 (en) * 2011-10-11 2018-06-12 Qiagen Gmbh Sample processing method and sample processing cartridge
EP3901280B1 (de) 2012-10-17 2025-03-12 10x Genomics Sweden AB Verfahren und produkt zur optimierung einer lokalisierten oder räumlichen detektion einer genexpression in einer gewebeprobe
JP2014176303A (ja) * 2013-03-13 2014-09-25 Seiko Epson Corp cDNAの合成方法
CN105473744B (zh) 2013-06-25 2020-06-19 普罗格诺西斯生物科学公司 采用微流控装置的空间编码生物分析
FI3901281T3 (fi) 2015-04-10 2023-01-31 Biologisten näytteiden spatiaalisesti eroteltu moninkertainen nukleiinihappoanalyysi
CN106867993A (zh) * 2017-02-17 2017-06-20 唐旌生物科技(上海)有限公司 一种逆转录方法、逆转录试剂盒及其应用
US12110541B2 (en) 2020-02-03 2024-10-08 10X Genomics, Inc. Methods for preparing high-resolution spatial arrays
US12031177B1 (en) 2020-06-04 2024-07-09 10X Genomics, Inc. Methods of enhancing spatial resolution of transcripts
US20240318162A1 (en) * 2021-07-01 2024-09-26 Cubit Diagnostics, Inc. Methods and reagents for analyzing nucleic acids

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE4034036C2 (de) * 1990-10-26 1994-03-03 Diagen Inst Molekularbio Vorrichtung und Verfahren zur Isolierung von Nukleinsäuren aus Zellsuspensionen
JPH04187077A (ja) * 1990-11-22 1992-07-03 Shimadzu Corp 核酸の抽出精製装置
JPH04360686A (ja) * 1991-06-04 1992-12-14 Tosoh Corp Dnaの精製方法
US5437976A (en) * 1991-08-08 1995-08-01 Arizona Board Of Regents, The University Of Arizona Multi-domain DNA ligands bound to a solid matrix for protein and nucleic acid affinity chromatography and processing of solid-phase DNA
BR9206705A (pt) * 1991-11-01 1995-11-21 Adelaide Children S Hospital Processo de amplificação de fase sólida
JPH08500722A (ja) * 1992-01-29 1996-01-30 日立化成工業株式会社 ポリヌクレオチド固定化担体
US5545528A (en) * 1993-04-15 1996-08-13 Hitachi Chemical Research Center Rapid screening method of gene amplification products in polypropylene plates
GB9314249D0 (en) * 1993-07-09 1993-08-18 Proofname Ltd Purification method and apparatus
GB9507238D0 (en) * 1995-04-07 1995-05-31 Isis Innovation Detecting dna sequence variations
US5989431A (en) * 1995-06-08 1999-11-23 Progen Industries Ltd Method and apparatus for DNA extraction
EP0814156B1 (de) * 1996-06-18 2003-03-05 The Institute Of Physical & Chemical Research Verfahren zur Reinigung von DNS

Also Published As

Publication number Publication date
EP1600512A1 (de) 2005-11-30
EP1042497A1 (de) 2000-10-11
EP1600512B1 (de) 2008-05-28
ATE506449T1 (de) 2011-05-15
JP2006051042A (ja) 2006-02-23
EP1865074A2 (de) 2007-12-12
DE69839572D1 (de) 2008-07-10
EP1865074A3 (de) 2008-02-27
JP4337796B2 (ja) 2009-09-30
WO1999032654A1 (en) 1999-07-01
DE69842238D1 (de) 2011-06-01
EP1865074B1 (de) 2011-04-20
JP2002505080A (ja) 2002-02-19
JP4983836B2 (ja) 2012-07-25
DE69839734D1 (de) 2008-08-28
JP4325114B2 (ja) 2009-09-02
ATE397086T1 (de) 2008-06-15
EP1042497B1 (de) 2008-07-16
EP1042497A4 (de) 2004-09-22
JP2009189368A (ja) 2009-08-27

Similar Documents

Publication Publication Date Title
ATE401415T1 (de) Direkte rt-pcr auf mikrotiterplatten mit immobilisierten oligonukleotid
JP5495399B2 (ja) 核酸を増幅および配列決定する方法
US10718014B2 (en) Thermo-controllable high-density chips for multiplex analyses
JP2007523627A6 (ja) 核酸を増幅および配列決定する方法
NO20021340L (no) Innretning for gjennomföring av kjemiske eller biologiske reaksjoner
EP1922664A2 (de) Verfahren zur amplifikation und sequenzierung von nukleinsäuren
ATE74023T1 (de) Reaktor zur internen temperatursteuerung durch hohle plattenwaermeaustauscher.
US20070148678A1 (en) Device and method for carrying out a nucleic acid test, and method for producing such a device
ATE401126T1 (de) Heizung von mehrkammerbehältern

Legal Events

Date Code Title Description
RER Ceased as to paragraph 5 lit. 3 law introducing patent treaties