ATE401415T1 - Direkte rt-pcr auf mikrotiterplatten mit immobilisierten oligonukleotid - Google Patents

Direkte rt-pcr auf mikrotiterplatten mit immobilisierten oligonukleotid

Info

Publication number
ATE401415T1
ATE401415T1 AT98964860T AT98964860T ATE401415T1 AT E401415 T1 ATE401415 T1 AT E401415T1 AT 98964860 T AT98964860 T AT 98964860T AT 98964860 T AT98964860 T AT 98964860T AT E401415 T1 ATE401415 T1 AT E401415T1
Authority
AT
Austria
Prior art keywords
pcr
microplates
immobilized
direct
filter plate
Prior art date
Application number
AT98964860T
Other languages
English (en)
Inventor
Masato Mitsuhashi
Original Assignee
Hitachi Chemical Co Ltd
Hitachi Chemical Res Ct Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hitachi Chemical Co Ltd, Hitachi Chemical Res Ct Inc filed Critical Hitachi Chemical Co Ltd
Application granted granted Critical
Publication of ATE401415T1 publication Critical patent/ATE401415T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/06—Lysis of microorganisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6851—Quantitative amplification

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Biophysics (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Medicinal Chemistry (AREA)
  • Mycology (AREA)
  • Virology (AREA)
  • Biomedical Technology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Saccharide Compounds (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
AT98964860T 1997-12-22 1998-12-22 Direkte rt-pcr auf mikrotiterplatten mit immobilisierten oligonukleotid ATE401415T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US6839497P 1997-12-22 1997-12-22
US7162798P 1998-01-16 1998-01-16

Publications (1)

Publication Number Publication Date
ATE401415T1 true ATE401415T1 (de) 2008-08-15

Family

ID=26748929

Family Applications (3)

Application Number Title Priority Date Filing Date
AT05017491T ATE397086T1 (de) 1997-12-22 1998-12-22 Reinigung von mrna auf mikroplatten
AT07016364T ATE506449T1 (de) 1997-12-22 1998-12-22 Direkte rt-pcr auf oligonukleotid-immobilisierten pcr-mikroplatten
AT98964860T ATE401415T1 (de) 1997-12-22 1998-12-22 Direkte rt-pcr auf mikrotiterplatten mit immobilisierten oligonukleotid

Family Applications Before (2)

Application Number Title Priority Date Filing Date
AT05017491T ATE397086T1 (de) 1997-12-22 1998-12-22 Reinigung von mrna auf mikroplatten
AT07016364T ATE506449T1 (de) 1997-12-22 1998-12-22 Direkte rt-pcr auf oligonukleotid-immobilisierten pcr-mikroplatten

Country Status (5)

Country Link
EP (3) EP1600512B1 (de)
JP (3) JP4325114B2 (de)
AT (3) ATE397086T1 (de)
DE (3) DE69839572D1 (de)
WO (1) WO1999032654A1 (de)

Families Citing this family (56)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6844158B1 (en) 1997-12-22 2005-01-18 Hitachi Chemical Co., Ltd. Direct RT-PCR on oligonucleotide-immobilized PCR microplates
US6686151B1 (en) 1998-02-06 2004-02-03 Digene Corporation Immunological detection of RNA:DNA hybrids on microarrays
US7399589B2 (en) 1998-02-06 2008-07-15 Digene Corporation Immunological detection of RNA:DNA hybrids on microarrays
US20050100943A1 (en) 2000-04-11 2005-05-12 Hideki Kambara Method of producing probe arrays for biological materials using fine particles
JP3746658B2 (ja) * 1999-04-12 2006-02-15 株式会社日立製作所 微粒子を用いたプローブアレーの作製方法及び装置
FR2798675B1 (fr) * 1999-09-16 2004-02-27 Centre Nat Rech Scient Procede et dispositif de fabrication d'un support porteur d'une pluralite de sequences polynucleotidiques et/ou peptidiques differentes
CA2318730C (en) * 1999-09-20 2008-01-22 Ortho-Clinical Diagnostics, Inc. A method for reverse transcribing rna isolated in dry form
US6642000B1 (en) * 1999-11-12 2003-11-04 University Of Chicago PCR amplification on microarrays of gel immobilized oligonucleotides
ITCA20000004A1 (it) * 2000-03-07 2001-09-07 Mario Raimondo Caria Metodo e sistema per la rivelazione simultanea e multipla con quantificazione della ibridizzazione di composti molecolari quali acidi nuclei
US6376191B1 (en) * 2000-03-22 2002-04-23 Mergen, Ltd. Microarray-based analysis of polynucleotide sequence variations
US7601497B2 (en) 2000-06-15 2009-10-13 Qiagen Gaithersburg, Inc. Detection of nucleic acids by target-specific hybrid capture method
US7439016B1 (en) 2000-06-15 2008-10-21 Digene Corporation Detection of nucleic acids by type-specific hybrid capture method
DE10033091A1 (de) * 2000-07-07 2002-01-31 Max Planck Gesellschaft Polymer-Chip
AU2000275323A1 (en) * 2000-09-27 2002-04-08 Centre National De La Recherche Scientifique (C.N.R.S.) Method and device for making a support bearing a plurality of different polynucleotide and/or peptide sequences
DE60143046D1 (de) 2000-10-31 2010-10-21 Hitachi Chemical Co Ltd Methode für das sammeln und das verwenden von kern-mrna
US7285199B2 (en) 2000-10-31 2007-10-23 Hitachi Chemical Research Center, Inc. Apparatus and method for electrophoretic microspot concentration
GB0105790D0 (en) * 2001-03-08 2001-04-25 Expresson Biosystems Ltd Detecting binding of mRNA to an oligonnucleotide array using RNA dependent nucleic acid modifying enzymes
AU2002318631B2 (en) * 2001-07-09 2007-08-02 Asahi Kasei Kabushiki Kaisha Method of purifying nucleic acid using nonwoven fabric and detection method
JP4471659B2 (ja) * 2002-01-09 2010-06-02 シスメックス株式会社 直接核酸増幅法
WO2003091407A2 (en) * 2002-04-24 2003-11-06 Hitachi Chemical Research Center, Inc. Device and method for high-throughput quantification of mrna from whole blood
US7745180B2 (en) 2002-04-24 2010-06-29 Hitachi Chemical Co., Ltd. Device and method for high-throughput quantification of mRNA from whole blood
US20040067492A1 (en) * 2002-10-04 2004-04-08 Allan Peng Reverse transcription on microarrays
WO2004070007A2 (en) 2003-01-29 2004-08-19 454 Corporation Method for preparing single-stranded dna libraries
CN1580283A (zh) * 2003-08-13 2005-02-16 清华大学 一种检测核酸分子的方法
DE10353887A1 (de) * 2003-11-18 2005-06-16 Febit Ag Hochparalleler DNA-Synthesizer auf Matrizenbasis
EP1548126A1 (de) * 2003-12-22 2005-06-29 Bio-Rad Pasteur Trägermaterial für Kontrollnukleinsäuren und Anwendung dieser zur Nukleinsäuredetektion.
US20050277121A1 (en) * 2004-06-11 2005-12-15 Ambion, Inc. Crude biological derivatives competent for nucleic acid detection
CN101116086A (zh) 2004-11-16 2008-01-30 伊路敏纳公司 读取编码微珠的方法和设备
JP2008527997A (ja) * 2005-01-21 2008-07-31 ザ ニューヨーク ブラッド センター インコーポレイテッド 核酸の抽出及び同定方法
JP2008538928A (ja) * 2005-04-28 2008-11-13 ヒタチ ケミカル リサーチ センター インコーポレイテッド マイクロプレートベースの反応において試薬の消費を最小限にする方法
FR2885140B1 (fr) * 2005-04-29 2010-12-31 Millipore Corp Procede de detection et de caracterisation de microorgarnismes sur une membrane.
JP4916689B2 (ja) * 2005-09-12 2012-04-18 住友ベークライト株式会社 Ｄｎａ鎖増幅方法
US7830575B2 (en) 2006-04-10 2010-11-09 Illumina, Inc. Optical scanner with improved scan time
US8088580B2 (en) 2006-06-07 2012-01-03 Sumitomo Bakelite Company, Ltd. RNA detection method
EP2064655A4 (de) 2006-07-12 2012-02-29 Color Savvy Systems Ltd Verfahren zum ableiten einheitlicher farbmessungen
AU2007281535A1 (en) * 2006-08-01 2008-02-07 Applied Biosystems, Llc. Detection of analytes and nucleic acids
TWI419974B (zh) 2008-10-27 2013-12-21 Qiagen Gaithersburg Inc 於自動平台上之快速結果雜交捕捉法
EP2425019B1 (de) 2009-05-01 2014-03-19 QIAGEN Gaithersburg, Inc. Ungezieltes verstärkungsverfahren zur erkennung von rna-spleissformen in einer probe
JP5729904B2 (ja) * 2009-06-02 2015-06-03 キヤノン株式会社 細胞から蛋白質、ｄｎａ、ｒｎａを調製する方法
EP2454378B1 (de) * 2009-07-17 2015-12-23 Canon U.S. Life Sciences, Inc. Verfahren und systeme für dna-isolation bei einer mikrofluidischen vorrichtung
JP5826752B2 (ja) 2009-09-14 2015-12-02 キアジェン ゲイサーズバーグ インコーポレイテッド 細胞学用培地に固定された組織サンプルから核酸またはタンパク質を回収するための組成物および方法
CN102822189B (zh) 2010-01-29 2016-06-29 奇亚根盖瑟斯堡股份有限公司 用于核酸的序列特异性纯化和多重复合体分析(multiplex analysis)的方法和组合物
US9605303B2 (en) 2010-01-29 2017-03-28 Qiagen Gaithersburg, Inc. Method of determining and confirming the presence of an HPV in a sample
US10787701B2 (en) 2010-04-05 2020-09-29 Prognosys Biosciences, Inc. Spatially encoded biological assays
US20190300945A1 (en) 2010-04-05 2019-10-03 Prognosys Biosciences, Inc. Spatially Encoded Biological Assays
KR101866401B1 (ko) 2010-04-05 2018-06-11 프로그노시스 바이오사이언스, 인코포레이티드 공간적으로 엔코딩된 생물학적 검정
JP2013528049A (ja) 2010-05-19 2013-07-08 キアゲン ガイサーズバーグ アイエヌシー． 核酸の配列特異的な精製及び多重分析のための方法及び組成物
US9376727B2 (en) 2010-05-25 2016-06-28 Qiagen Gaithersburg, Inc. Fast results hybrid capture assay and associated strategically truncated probes
CN103597095B (zh) 2011-02-24 2016-08-17 奇亚根盖瑟斯堡股份有限公司 用于检测hpv核酸的材料和方法
GB201106254D0 (en) 2011-04-13 2011-05-25 Frisen Jonas Method and product
EP3663408B1 (de) * 2011-10-11 2024-05-01 QIAGEN GmbH Probenverarbeitungsverfahren und probenverarbeitungskartusche
WO2014060483A1 (en) 2012-10-17 2014-04-24 Spatial Transcriptomics Ab Methods and product for optimising localised or spatial detection of gene expression in a tissue sample
JP2014176303A (ja) 2013-03-13 2014-09-25 Seiko Epson Corp ｃＤＮＡの合成方法
US9868979B2 (en) 2013-06-25 2018-01-16 Prognosys Biosciences, Inc. Spatially encoded biological assays using a microfluidic device
ES2955916T3 (es) 2015-04-10 2023-12-11 Spatial Transcriptomics Ab Análisis múltiplex de especímenes biológicos de ácidos nucleicos espacialmente distinguidos
CN106867993A (zh) * 2017-02-17 2017-06-20 唐旌生物科技（上海）有限公司 一种逆转录方法、逆转录试剂盒及其应用

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE4034036C2 (de) * 1990-10-26 1994-03-03 Diagen Inst Molekularbio Vorrichtung und Verfahren zur Isolierung von Nukleinsäuren aus Zellsuspensionen
JPH04187077A (ja) * 1990-11-22 1992-07-03 Shimadzu Corp 核酸の抽出精製装置
JPH04360686A (ja) * 1991-06-04 1992-12-14 Tosoh Corp Ｄｎａの精製方法
US5437976A (en) * 1991-08-08 1995-08-01 Arizona Board Of Regents, The University Of Arizona Multi-domain DNA ligands bound to a solid matrix for protein and nucleic acid affinity chromatography and processing of solid-phase DNA
BR9206705A (pt) * 1991-11-01 1995-11-21 Adelaide Children S Hospital Processo de amplificação de fase sólida
KR100289793B1 (ko) * 1992-01-29 2001-12-01 테츠오 토미나가 폴리누클레오티드고정지지체
US5545528A (en) * 1993-04-15 1996-08-13 Hitachi Chemical Research Center Rapid screening method of gene amplification products in polypropylene plates
GB9314249D0 (en) * 1993-07-09 1993-08-18 Proofname Ltd Purification method and apparatus
GB9507238D0 (en) * 1995-04-07 1995-05-31 Isis Innovation Detecting dna sequence variations
EP0830364A1 (de) * 1995-06-08 1998-03-25 Progen Industries Limited Verfahren und vorrichtung zur dns-extraktion
DE69719409T2 (de) * 1996-06-18 2004-01-15 Rikagaku Kenkyusho Verfahren zur Reinigung von DNS

Also Published As

Publication number Publication date
JP4983836B2 (ja) 2012-07-25
ATE397086T1 (de) 2008-06-15
EP1600512A1 (de) 2005-11-30
EP1865074A2 (de) 2007-12-12
JP4325114B2 (ja) 2009-09-02
DE69839734D1 (de) 2008-08-28
DE69839572D1 (de) 2008-07-10
JP2009189368A (ja) 2009-08-27
JP4337796B2 (ja) 2009-09-30
EP1600512B1 (de) 2008-05-28
DE69842238D1 (de) 2011-06-01
EP1042497B1 (de) 2008-07-16
EP1042497A1 (de) 2000-10-11
EP1865074B1 (de) 2011-04-20
EP1865074A3 (de) 2008-02-27
WO1999032654A1 (en) 1999-07-01
JP2002505080A (ja) 2002-02-19
JP2006051042A (ja) 2006-02-23
ATE506449T1 (de) 2011-05-15
EP1042497A4 (de) 2004-09-22

Similar Documents

Publication Publication Date Title
ATE401415T1 (de) Direkte rt-pcr auf mikrotiterplatten mit immobilisierten oligonukleotid
JP5495399B2 (ja) 核酸を増幅および配列決定する方法
US9909171B2 (en) Thermo-controllable high-density chips for multiplex analyses
JP4205434B2 (ja) マルチフォーマットサンプル処理装置およびサンプル処理装置を使用する方法
JP2007523627A6 (ja) 核酸を増幅および配列決定する方法
NO20021340L (no) Innretning for gjennomföring av kjemiske eller biologiske reaksjoner
WO2007086935A2 (en) Methods of amplifying and sequencing nucleic acids
US20070148678A1 (en) Device and method for carrying out a nucleic acid test, and method for producing such a device
WO2003039749A3 (en) Sample carrier comprising a structural array for archiving sample material
DE3869582D1 (de) Reaktor zur internen temperatursteuerung durch hohle plattenwaermeaustauscher.
Belgrader et al. Automated sample processing using robotics for genetic typing of short tandem repeat polymorphisms by capillary electrophoresis
WO2003000839A3 (en) Clinical assay for nucleic acids amplified in solid matrices to produce colonies of the progeny of individual target molecules
AR018049A1 (es) Un equipo para llevar a cabo procedimientos analiticos quimicos, biologicos y/o bioquimicos

Legal Events

Date Code Title Description
RER Ceased as to paragraph 5 lit. 3 law introducing patent treaties