DE69842238D1 - Direkte RT-PCR auf Oligonukleotid-immobilisierten PCR-Mikroplatten - Google Patents

Direkte RT-PCR auf Oligonukleotid-immobilisierten PCR-Mikroplatten

Info

Publication number
DE69842238D1
DE69842238D1 DE69842238T DE69842238T DE69842238D1 DE 69842238 D1 DE69842238 D1 DE 69842238D1 DE 69842238 T DE69842238 T DE 69842238T DE 69842238 T DE69842238 T DE 69842238T DE 69842238 D1 DE69842238 D1 DE 69842238D1
Authority
DE
Germany
Prior art keywords
pcr
microplates
immobilized
oligonucleotide
filter plate
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69842238T
Other languages
English (en)
Inventor
Masato Mitsuhashi
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Showa Denko Materials Co ltd
Showa Denko Materials America Inc
Original Assignee
Hitachi Chemical Co Ltd
Hitachi Chemical Research Center Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hitachi Chemical Co Ltd, Hitachi Chemical Research Center Inc filed Critical Hitachi Chemical Co Ltd
Application granted granted Critical
Publication of DE69842238D1 publication Critical patent/DE69842238D1/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/06—Lysis of microorganisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6851—Quantitative amplification

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Biophysics (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Medicinal Chemistry (AREA)
  • Mycology (AREA)
  • Virology (AREA)
  • Biomedical Technology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
DE69842238T 1997-12-22 1998-12-22 Direkte RT-PCR auf Oligonukleotid-immobilisierten PCR-Mikroplatten Expired - Lifetime DE69842238D1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US6839497P 1997-12-22 1997-12-22
US7162798P 1998-01-16 1998-01-16

Publications (1)

Publication Number Publication Date
DE69842238D1 true DE69842238D1 (de) 2011-06-01

Family

ID=26748929

Family Applications (3)

Application Number Title Priority Date Filing Date
DE69839572T Expired - Lifetime DE69839572D1 (de) 1997-12-22 1998-12-22 Reinigung von mRNA auf Mikroplatten
DE69842238T Expired - Lifetime DE69842238D1 (de) 1997-12-22 1998-12-22 Direkte RT-PCR auf Oligonukleotid-immobilisierten PCR-Mikroplatten
DE69839734T Expired - Lifetime DE69839734D1 (de) 1997-12-22 1998-12-22 Direkte rt-pcr auf mikrotiterplatten mit immobilisierten oligonukleotid

Family Applications Before (1)

Application Number Title Priority Date Filing Date
DE69839572T Expired - Lifetime DE69839572D1 (de) 1997-12-22 1998-12-22 Reinigung von mRNA auf Mikroplatten

Family Applications After (1)

Application Number Title Priority Date Filing Date
DE69839734T Expired - Lifetime DE69839734D1 (de) 1997-12-22 1998-12-22 Direkte rt-pcr auf mikrotiterplatten mit immobilisierten oligonukleotid

Country Status (5)

Country Link
EP (3) EP1865074B1 (de)
JP (3) JP4325114B2 (de)
AT (3) ATE506449T1 (de)
DE (3) DE69839572D1 (de)
WO (1) WO1999032654A1 (de)

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DE60143046D1 (de) * 2000-10-31 2010-10-21 Hitachi Chemical Co Ltd Methode für das sammeln und das verwenden von kern-mrna
GB0105790D0 (en) * 2001-03-08 2001-04-25 Expresson Biosystems Ltd Detecting binding of mRNA to an oligonnucleotide array using RNA dependent nucleic acid modifying enzymes
CN100487116C (zh) * 2001-07-09 2009-05-13 旭化成株式会社 应用非织造织物纯化和检测核酸的方法
WO2003060116A1 (fr) * 2002-01-09 2003-07-24 Sysmex Corporation Procede de detection d'acides nucleiques et systeme associe
US7745180B2 (en) * 2002-04-24 2010-06-29 Hitachi Chemical Co., Ltd. Device and method for high-throughput quantification of mRNA from whole blood
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US20040067492A1 (en) * 2002-10-04 2004-04-08 Allan Peng Reverse transcription on microarrays
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US20050277121A1 (en) * 2004-06-11 2005-12-15 Ambion, Inc. Crude biological derivatives competent for nucleic acid detection
AU2005307746B2 (en) 2004-11-16 2011-05-12 Illumina, Inc. And methods and apparatus for reading coded microbeads
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WO2006116708A2 (en) * 2005-04-28 2006-11-02 Hitachi Chemical Research Center, Inc. Method of minimizing reagent consumption in microplate-based reactions
FR2885140B1 (fr) * 2005-04-29 2010-12-31 Millipore Corp Procede de detection et de caracterisation de microorgarnismes sur une membrane.
JP4916689B2 (ja) * 2005-09-12 2012-04-18 住友ベークライト株式会社 Ｄｎａ鎖増幅方法
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JP5815518B2 (ja) * 2009-07-17 2015-11-17 キヤノン ユー．エス． ライフ サイエンシズ， インコーポレイテッドＣａｎｏｎ Ｕ．Ｓ． Ｌｉｆｅ Ｓｃｉｅｎｃｅｓ， Ｉｎｃ． マイクロ流体装置においてｄｎａを単離する方法及びシステム
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Also Published As

Publication number Publication date
JP2009189368A (ja) 2009-08-27
JP2002505080A (ja) 2002-02-19
ATE401415T1 (de) 2008-08-15
EP1865074A3 (de) 2008-02-27
EP1042497A1 (de) 2000-10-11
EP1042497B1 (de) 2008-07-16
EP1042497A4 (de) 2004-09-22
DE69839572D1 (de) 2008-07-10
WO1999032654A1 (en) 1999-07-01
ATE506449T1 (de) 2011-05-15
EP1600512A1 (de) 2005-11-30
EP1600512B1 (de) 2008-05-28
JP4983836B2 (ja) 2012-07-25
EP1865074B1 (de) 2011-04-20
JP2006051042A (ja) 2006-02-23
ATE397086T1 (de) 2008-06-15
DE69839734D1 (de) 2008-08-28
JP4337796B2 (ja) 2009-09-30
JP4325114B2 (ja) 2009-09-02
EP1865074A2 (de) 2007-12-12

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