DE60143046D1 - Methode für das sammeln und das verwenden von kern-mrna - Google Patents

Methode für das sammeln und das verwenden von kern-mrna

Info

Publication number
DE60143046D1
DE60143046D1 DE60143046T DE60143046T DE60143046D1 DE 60143046 D1 DE60143046 D1 DE 60143046D1 DE 60143046 T DE60143046 T DE 60143046T DE 60143046 T DE60143046 T DE 60143046T DE 60143046 D1 DE60143046 D1 DE 60143046D1
Authority
DE
Germany
Prior art keywords
mrna
cell
collecting
solution
novel method
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE60143046T
Other languages
English (en)
Inventor
Masato Mitsuhashi
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Showa Denko Materials Co ltd
Showa Denko Materials America Inc
Original Assignee
Hitachi Chemical Co Ltd
Hitachi Chemical Research Center Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hitachi Chemical Co Ltd, Hitachi Chemical Research Center Inc filed Critical Hitachi Chemical Co Ltd
Application granted granted Critical
Publication of DE60143046D1 publication Critical patent/DE60143046D1/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6809—Methods for determination or identification of nucleic acids involving differential detection
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1017—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by filtration, e.g. using filters, frits, membranes

Landscapes

  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Immunology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
DE60143046T 2000-10-31 2001-10-25 Methode für das sammeln und das verwenden von kern-mrna Expired - Lifetime DE60143046D1 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US24467200P 2000-10-31 2000-10-31
US30803801P 2001-07-26 2001-07-26
PCT/US2001/049498 WO2002066637A2 (en) 2000-10-31 2001-10-25 Method for collecting and using nuclear mrna

Publications (1)

Publication Number Publication Date
DE60143046D1 true DE60143046D1 (de) 2010-10-21

Family

ID=26936712

Family Applications (1)

Application Number Title Priority Date Filing Date
DE60143046T Expired - Lifetime DE60143046D1 (de) 2000-10-31 2001-10-25 Methode für das sammeln und das verwenden von kern-mrna

Country Status (7)

Country Link
US (1) US7374881B2 (de)
EP (1) EP1330518B1 (de)
JP (1) JP5416326B2 (de)
CN (1) CN1294262C (de)
AT (1) ATE480618T1 (de)
DE (1) DE60143046D1 (de)
WO (1) WO2002066637A2 (de)

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US6844158B1 (en) * 1997-12-22 2005-01-18 Hitachi Chemical Co., Ltd. Direct RT-PCR on oligonucleotide-immobilized PCR microplates
WO2002042500A2 (en) 2000-10-31 2002-05-30 Hitachi Chemical Research Center, Inc. Apparatus and method for electrophoretic microspot concentration
US20050277121A1 (en) * 2004-06-11 2005-12-15 Ambion, Inc. Crude biological derivatives competent for nucleic acid detection
EP1888089B9 (de) * 2005-04-28 2012-08-08 Hitachi Chemical Research Center, Inc. Ex-vivo-genexpression in vollblut als modell für die beurteilung von individueller variation gegenüber nahrungszusätzen
GB0522193D0 (en) 2005-10-31 2005-12-07 Axis Shield Asa Method
JP2009536021A (ja) * 2006-04-07 2009-10-08 日立化成工業株式会社 関節リウマチの患者の末梢血白血球におけるＦＣ受容体媒介性腫瘍壊死因子スーパーファミリ及びケモカインのｍＲＮＡ発現の増大
WO2007117611A2 (en) * 2006-04-07 2007-10-18 Hitachi Chemical Co., Ltd. Enhanced t cell receptor-mediated tumor necrosis factor superfamily and chemokine mrna expression in peripheral blood leukocytes in patients with crohn's disease
WO2009070442A2 (en) 2007-11-14 2009-06-04 Hitachi Chemical Co. Ltd. Fc receptor-mediated tumor necrosis factor superfamily mrna expression in peripheral blood leukocytes
US8546127B2 (en) * 2008-06-30 2013-10-01 General Electric Company Bacteria/RNA extraction device
US8304185B2 (en) 2009-07-17 2012-11-06 Canon U.S. Life Sciences, Inc. Methods and systems for DNA isolation on a microfluidic device
US20100062946A1 (en) * 2008-09-08 2010-03-11 Lis John T Genome-wide method for mapping of engaged rna polymerases quantitatively and at high resolution
CN101497925B (zh) * 2008-12-17 2012-05-02 安徽医科大学 Leber氏遗传性视神经病变相关mtDNA突变位点集成检测基因芯片及其制备与应用
JP5729904B2 (ja) * 2009-06-02 2015-06-03 キヤノン株式会社 細胞から蛋白質、ｄｎａ、ｒｎａを調製する方法
EP2454378B1 (de) * 2009-07-17 2015-12-23 Canon U.S. Life Sciences, Inc. Verfahren und systeme für dna-isolation bei einer mikrofluidischen vorrichtung
BR112012002401A2 (pt) * 2009-08-04 2019-09-24 Du Pont processo para coletar ácidos nucleicos de micro-organismos a partir de uma amostra particulada
CN102639716A (zh) 2009-12-04 2012-08-15 株式会社日立制作所 使用二维cDNA文库的基因表达解析方法
JP5755326B2 (ja) 2010-06-11 2015-07-29 日立化成株式会社 腎機能の特徴を決定する方法
WO2011156734A2 (en) * 2010-06-11 2011-12-15 Hitachi Chemical Co., Ltd. Method of characterizing vascular diseases
US20110312628A1 (en) * 2010-06-17 2011-12-22 Geneasys Pty Ltd Microfluidic device with mst layer and overlying cap
JP5823031B2 (ja) 2011-06-10 2015-11-25 日立化成株式会社 小胞捕捉デバイスおよびそれを用いるための方法
DE212013000295U1 (de) 2013-05-06 2016-02-02 Hitachi Chemical Co. America, Ltd. Vorrichtungen zum Einfangen von Zielmolekülen
US10266895B2 (en) 2014-11-05 2019-04-23 Hitachi Chemical Company Ltd. Exosomes and microvesicles in intestinal luminal fluids and stool and use of same for the assessment of inflammatory bowel disease
EP3218521B1 (de) 2014-11-12 2019-12-25 Hitachi Chemical Co., Ltd. Verfahren zur diagnose von organverletzung
DE112016003948T5 (de) 2015-08-31 2018-05-09 City Of Sapporo Molekulare verfahren zum beurteilen einer urothelialen erkrankung
JP2019507588A (ja) * 2016-01-08 2019-03-22 パスクエスト 無細胞体液中の核酸消化酵素に対する宿主核酸の到達性の調整
CN115247204A (zh) * 2022-02-15 2022-10-28 温州医科大学 一种基于qRT-PCR技术检测lncRNA亚细胞定位的方法

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US4139346A (en) * 1977-11-28 1979-02-13 Enzo Bio Chem Incorporated Nucleic acid and protein binding paper
US4906561A (en) * 1981-09-14 1990-03-06 Thornthwaite Jerry T Nuclear isolation medium and procedure for separating cell nuclei
US4430278A (en) 1982-02-01 1984-02-07 Jones Sr John L Process for increasing the permeability of plastic membrane
US5057438A (en) 1986-09-24 1991-10-15 Agency Of Industrial Science And Technology Electrophoretic antigen-antibody determination with laminate of multiple membranes
DE3715856A1 (de) 1987-05-12 1988-12-01 Biometra Biomedizinische Analy Vorrichtung zum konzentrieren von in einer fluessigkeit befindlichen, elektrisch geladenen - insbesondere aus einem gel eluierten - makromolekuelen
JPH01179090A (ja) * 1988-01-06 1989-07-17 Yamaha Corp 自動伴奏装置
US5158661A (en) 1991-03-22 1992-10-27 Fotodyne Incorporated Electrophoresis temperature control apparatus
EP0587951B1 (de) * 1992-09-18 1997-12-17 AMERSHAM INTERNATIONAL plc Verfahren zum Auffangen von Zellkernen und dafür verwendete Vorrichtung
US5955272A (en) * 1993-02-26 1999-09-21 University Of Massachusetts Detection of individual gene transcription and splicing
US6113763A (en) 1996-11-04 2000-09-05 Board Of Trustee Operating Michigan State University Method for measuring cellular chemical profiles
JP2002515754A (ja) * 1996-11-12 2002-05-28 キュービーアイ エンタプライジズ リミテッド 翻訳的に制御される遺伝子の同定方法
DE69723297D1 (de) * 1996-12-11 2003-08-07 Amersham Plc Little Chalfont Selektives verfahren zur lyse von zellen
US5964997A (en) 1997-03-21 1999-10-12 Sarnoff Corporation Balanced asymmetric electronic pulse patterns for operating electrode-based pumps
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DE19725190A1 (de) 1997-06-14 1998-12-17 Innova Gmbh Vorrichtungen mit integrierten Elektroden aus elektrisch leitfähigen Kunststoffen
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Also Published As

Publication number Publication date
JP5416326B2 (ja) 2014-02-12
EP1330518B1 (de) 2010-09-08
WO2002066637A2 (en) 2002-08-29
US20040072193A1 (en) 2004-04-15
EP1330518A2 (de) 2003-07-30
CN1606621A (zh) 2005-04-13
CN1294262C (zh) 2007-01-10
WO2002066637A8 (en) 2003-10-30
JP2004519234A (ja) 2004-07-02
WO2002066637A3 (en) 2003-05-22
ATE480618T1 (de) 2010-09-15
US7374881B2 (en) 2008-05-20

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