ATE480618T1 - Methode für das sammeln und das verwenden von kern-mrna - Google Patents

Methode für das sammeln und das verwenden von kern-mrna

Info

Publication number
ATE480618T1
ATE480618T1 AT01270141T AT01270141T ATE480618T1 AT E480618 T1 ATE480618 T1 AT E480618T1 AT 01270141 T AT01270141 T AT 01270141T AT 01270141 T AT01270141 T AT 01270141T AT E480618 T1 ATE480618 T1 AT E480618T1
Authority
AT
Austria
Prior art keywords
mrna
cell
collecting
solution
novel method
Prior art date
Application number
AT01270141T
Other languages
English (en)
Inventor
Masato Mitsuhashi
Original Assignee
Hitachi Chemical Res Ct Inc
Hitachi Chemical Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hitachi Chemical Res Ct Inc, Hitachi Chemical Co Ltd filed Critical Hitachi Chemical Res Ct Inc
Application granted granted Critical
Publication of ATE480618T1 publication Critical patent/ATE480618T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6809—Methods for determination or identification of nucleic acids involving differential detection
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1017—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by filtration, e.g. using filters, frits, membranes

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Saccharide Compounds (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
AT01270141T 2000-10-31 2001-10-25 Methode für das sammeln und das verwenden von kern-mrna ATE480618T1 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US24467200P 2000-10-31 2000-10-31
US30803801P 2001-07-26 2001-07-26
PCT/US2001/049498 WO2002066637A2 (en) 2000-10-31 2001-10-25 Method for collecting and using nuclear mrna

Publications (1)

Publication Number Publication Date
ATE480618T1 true ATE480618T1 (de) 2010-09-15

Family

ID=26936712

Family Applications (1)

Application Number Title Priority Date Filing Date
AT01270141T ATE480618T1 (de) 2000-10-31 2001-10-25 Methode für das sammeln und das verwenden von kern-mrna

Country Status (7)

Country Link
US (1) US7374881B2 (de)
EP (1) EP1330518B1 (de)
JP (1) JP5416326B2 (de)
CN (1) CN1294262C (de)
AT (1) ATE480618T1 (de)
DE (1) DE60143046D1 (de)
WO (1) WO2002066637A2 (de)

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GB0522193D0 (en) * 2005-10-31 2005-12-07 Axis Shield Asa Method
ATE518011T1 (de) * 2006-04-07 2011-08-15 Hitachi Chemical Co Ltd Erhöhte expression von t- zellenrezeptorvermittelter tumornekrosefaktorsuperfamilie und chemokin-mrna in peripheren blut leukozyten bei patienten mit morbus& xa;crohn
JP2009536021A (ja) * 2006-04-07 2009-10-08 日立化成工業株式会社 関節リウマチの患者の末梢血白血球におけるＦＣ受容体媒介性腫瘍壊死因子スーパーファミリ及びケモカインのｍＲＮＡ発現の増大
EP2209919A4 (de) 2007-11-14 2011-04-13 Hitachi Chemical Co Ltd Fc-rezeptor-vermittelter-tumornekrosefaktor-superfamilie-mrna-expression in peripheren blutleukozyten
US8546127B2 (en) 2008-06-30 2013-10-01 General Electric Company Bacteria/RNA extraction device
US8304185B2 (en) 2009-07-17 2012-11-06 Canon U.S. Life Sciences, Inc. Methods and systems for DNA isolation on a microfluidic device
US20100062946A1 (en) * 2008-09-08 2010-03-11 Lis John T Genome-wide method for mapping of engaged rna polymerases quantitatively and at high resolution
CN101497925B (zh) * 2008-12-17 2012-05-02 安徽医科大学 Leber氏遗传性视神经病变相关mtDNA突变位点集成检测基因芯片及其制备与应用
JP5729904B2 (ja) * 2009-06-02 2015-06-03 キヤノン株式会社 細胞から蛋白質、ｄｎａ、ｒｎａを調製する方法
EP2454378B1 (de) * 2009-07-17 2015-12-23 Canon U.S. Life Sciences, Inc. Verfahren und systeme für dna-isolation bei einer mikrofluidischen vorrichtung
MX2012001236A (es) * 2009-08-04 2012-03-16 Du Pont Proceso y dispositivo para recolectar acidos nucleicos de microorganismos de una muestra en particulas.
US20120245053A1 (en) * 2009-12-04 2012-09-27 Hitachi, Ltd. GENE EXPRESSION ANALYSIS METHOD USING TWO DIMENSIONAL cDNA LIBRARY
JP5755326B2 (ja) 2010-06-11 2015-07-29 日立化成株式会社 腎機能の特徴を決定する方法
WO2011156734A2 (en) * 2010-06-11 2011-12-15 Hitachi Chemical Co., Ltd. Method of characterizing vascular diseases
US20110312554A1 (en) * 2010-06-17 2011-12-22 Geneasys Pty Ltd Microfluidic device with dialysis device, loc and interconnecting cap
US9719129B2 (en) 2011-06-10 2017-08-01 Hitachi Chemical Co., Ltd. Methods for isolating vesicles from biological fluids
JP6297676B2 (ja) 2013-05-06 2018-03-20 日立化成株式会社 標的分子を捕捉するためのデバイス及び方法
US10266895B2 (en) 2014-11-05 2019-04-23 Hitachi Chemical Company Ltd. Exosomes and microvesicles in intestinal luminal fluids and stool and use of same for the assessment of inflammatory bowel disease
US10370719B2 (en) 2014-11-12 2019-08-06 Hitachi Chemical Co., Ltd. Method and device for diagnosing organ injury
DE112016003948T5 (de) 2015-08-31 2018-05-09 City Of Sapporo Molekulare verfahren zum beurteilen einer urothelialen erkrankung
US11293049B2 (en) * 2016-01-08 2022-04-05 Pathoquest Modulation of accessibility of host nucleic acids to nucleic acid digesting enzymes in acellular biological fluids
CN115247204A (zh) * 2022-02-15 2022-10-28 温州医科大学 一种基于qRT-PCR技术检测lncRNA亚细胞定位的方法
CN115508269A (zh) * 2022-10-20 2022-12-23 上海华简检测技术有限公司 一种细胞处理剂定量取样对比分析法

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Also Published As

Publication number Publication date
CN1606621A (zh) 2005-04-13
DE60143046D1 (de) 2010-10-21
EP1330518B1 (de) 2010-09-08
US7374881B2 (en) 2008-05-20
WO2002066637A2 (en) 2002-08-29
CN1294262C (zh) 2007-01-10
WO2002066637A8 (en) 2003-10-30
JP5416326B2 (ja) 2014-02-12
EP1330518A2 (de) 2003-07-30
JP2004519234A (ja) 2004-07-02
US20040072193A1 (en) 2004-04-15
WO2002066637A3 (en) 2003-05-22

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