DE69723297D1 - Selektives verfahren zur lyse von zellen - Google Patents

Selektives verfahren zur lyse von zellen

Info

Publication number
DE69723297D1
DE69723297D1 DE69723297T DE69723297T DE69723297D1 DE 69723297 D1 DE69723297 D1 DE 69723297D1 DE 69723297 T DE69723297 T DE 69723297T DE 69723297 T DE69723297 T DE 69723297T DE 69723297 D1 DE69723297 D1 DE 69723297D1
Authority
DE
Germany
Prior art keywords
lysis
cells
selective method
selective
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69723297T
Other languages
English (en)
Inventor
Margaret Raybuck
Alison Sweet
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
GE Healthcare Ltd
Original Assignee
Amersham PLC
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Amersham PLC filed Critical Amersham PLC
Application granted granted Critical
Publication of DE69723297D1 publication Critical patent/DE69723297D1/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/06—Lysis of microorganisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Immunology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Urology & Nephrology (AREA)
  • Physics & Mathematics (AREA)
  • Hematology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Cell Biology (AREA)
  • Mycology (AREA)
  • Virology (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Food Science & Technology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
DE69723297T 1996-12-11 1997-12-10 Selektives verfahren zur lyse von zellen Expired - Lifetime DE69723297D1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP96309016 1996-12-11
PCT/GB1997/003402 WO1998026284A1 (en) 1996-12-11 1997-12-10 Selective lysis of cells

Publications (1)

Publication Number Publication Date
DE69723297D1 true DE69723297D1 (de) 2003-08-07

Family

ID=8225182

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69723297T Expired - Lifetime DE69723297D1 (de) 1996-12-11 1997-12-10 Selektives verfahren zur lyse von zellen

Country Status (5)

Country Link
EP (1) EP0944829B1 (de)
JP (1) JP2001506005A (de)
CA (1) CA2274793A1 (de)
DE (1) DE69723297D1 (de)
WO (1) WO1998026284A1 (de)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7122309B2 (en) * 2000-01-15 2006-10-17 Avigenics, Inc. High throughput screening assay for detecting a DNA sequence
FR2813891B1 (fr) * 2000-09-14 2005-01-14 Immunotech Sa Reactif multifonctionnel pour erythrocytes mettant en jeu des carbamates et applications
US7374881B2 (en) * 2000-10-31 2008-05-20 Hitachi Chemical Research Center, Inc. Method for collecting and using nuclear mRNA
EP1448799B2 (de) 2001-11-28 2018-05-16 Life Technologies Corporation Verfahren zur selektiven Nukleinsäureisolierung
US20030215845A1 (en) * 2002-02-19 2003-11-20 Bille Todd William Selective extraction of DNA from groups of cells
US20070196820A1 (en) * 2005-04-05 2007-08-23 Ravi Kapur Devices and methods for enrichment and alteration of cells and other particles
WO2009058997A2 (en) * 2007-11-01 2009-05-07 Biocept Inc. Non-invasive isolation of fetal nucleic acid
CN102282176A (zh) * 2008-07-18 2011-12-14 诺华有限公司 由母体全血进行非侵入性胎儿RhD基因型分型
SG175282A1 (en) 2009-04-21 2011-11-28 Genetic Technologies Ltd Methods for obtaining fetal genetic material
JP5729904B2 (ja) * 2009-06-02 2015-06-03 キヤノン株式会社 細胞から蛋白質、ｄｎａ、ｒｎａを調製する方法
WO2017161182A1 (en) * 2016-03-18 2017-09-21 Beckman Coulter, Inc. Subcellular localization of target analytes
US20210062183A1 (en) * 2018-01-08 2021-03-04 Cradle Genomics, Inc. Methods and kits for nucleic acid isolation

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4751179A (en) * 1984-05-31 1988-06-14 Coulter Electronics, Inc. Method and reagents for differential determination of four populations of leukocytes in blood
DE69223617T2 (de) * 1992-09-18 1998-04-09 Amersham Int Plc Verfahren zum Auffangen von Zellkernen und dafür verwendete Vorrichtung
US5457024A (en) * 1993-01-22 1995-10-10 Aprogenex, Inc. Isolation of fetal erythrocytes
US5559037A (en) * 1994-12-15 1996-09-24 Abbott Laboratories Method for rapid and simultaneous analysis of nucleated red blood cells

Also Published As

Publication number Publication date
CA2274793A1 (en) 1998-06-18
WO1998026284A1 (en) 1998-06-18
EP0944829B1 (de) 2003-07-02
JP2001506005A (ja) 2001-05-08
EP0944829A1 (de) 1999-09-29

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Legal Events

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