WO2017148426A1 - Procédé de détermination de l'empreinte d'une composition de médecine chinoise - Google Patents

Procédé de détermination de l'empreinte d'une composition de médecine chinoise Download PDF

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WO2017148426A1
WO2017148426A1 PCT/CN2017/075513 CN2017075513W WO2017148426A1 WO 2017148426 A1 WO2017148426 A1 WO 2017148426A1 CN 2017075513 W CN2017075513 W CN 2017075513W WO 2017148426 A1 WO2017148426 A1 WO 2017148426A1
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acid
solution
methanol
preparation
weigh
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PCT/CN2017/075513
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Chinese (zh)
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毕丹
陈育鹏
赵倩
叶玉廷
张水英
任晋
魏峰
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石家庄以岭药业股份有限公司
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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N2030/022—Column chromatography characterised by the kind of separation mechanism
    • G01N2030/027—Liquid chromatography

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  • the invention relates to a method for determining a fingerprint of a traditional Chinese medicine composition.
  • the fingerprint of traditional Chinese medicine refers to the chromatogram or spectrum of some Chinese herbal medicines or traditional Chinese medicine preparations which have been subjected to proper analysis and can be marked with chemical characteristics.
  • the fingerprint of traditional Chinese medicine is a comprehensive and quantifiable method for identification. It is based on the study of the chemical composition system of traditional Chinese medicine, and is mainly used to evaluate the authenticity, superiority and stability of the quality of Chinese herbal medicines and semi-finished products of traditional Chinese medicine preparations. "Integrality” and "fuzziness" are distinctive features.
  • the use of fingerprints as a quality control method for extracts of traditional Chinese medicines (natural medicines) and their preparations has become an international consensus, and various fingerprint control technology systems that conform to the characteristics of traditional Chinese medicines (natural medicines) are being researched and established.
  • the US Food and Drug Administration allows chromatographic fingerprinting in herbal health product declarations; the World Health Organization (WHO) also provides guidelines for herbal evaluation in 1996 that provide chromatographic fingerprints if the active ingredients of the herbs are not clear.
  • WHO World Health Organization
  • the European Community also said in the herbal quality guidelines that it is not enough to determine the stability of an active ingredient alone, because the herbs and their preparations are active as a whole.
  • Chromatographic fingerprints especially the sharp fingerprints of thin-layer chromatography, are useful.
  • the application of foreign fingerprints aims to solve the problem of the quality of botanical drug detection and the difference in batch quality between products with complex composition and unclear active ingredients.
  • High-performance liquid chromatography (HPLC) fingerprint with high resolution which can separate complex chemical components to form different chromatograms.
  • the height and peak area of these peaks represent various Different chemical compositions and their contents. It can be seen that the fingerprint development of traditional Chinese medicine is further developed than the DNA fingerprinting: not only the characteristic manifestation (the number and relative position of various chemical components - retention time) can be used for qualitative identification, but also the concept of quantity. .
  • the height and peak area of the peak indicate the content of a certain chemical component, and the ratio of the peak height (or peak area) of each peak reflects the relative content of various chemical components; the introduction of the concept of quantity, the combination of qualitative and quantitative It can give greater effect to the fingerprint of traditional Chinese medicine; the fingerprint of traditional Chinese medicine can not only identify the “uniqueness” of individuals and certain species, but also link the characteristics of “quantity” with other systems. Therefore, the fingerprint of traditional Chinese medicine is not only a quality control mode and technology of traditional Chinese medicine, but also can be developed into a research system and research mode that uses various fingerprints to carry out traditional Chinese medicine theory (complex system) and new drug development. The fingerprint should be fingerprinted, ie: (1) strong.
  • the fingerprints developed should be unique to the traditional Chinese medicine and distinguish it from other traditional Chinese medicines.
  • the chemical information reflected is highly selective; (2) the stability is good. That is, the fingerprint of traditional Chinese medicine should be the commonality from multiple batches of a certain Chinese medicine, and the common peak or characteristic peak in the map should be relatively stable; (3) good reproducibility.
  • the fingerprints developed should be able to reproduce the fingerprint features (such as the number, size, location, etc. of the common peaks) under specified conditions, and the error should be within the allowable range. Only in this way can the fingerprints developed have practical value to effectively control the quality of the drugs.
  • the UPLC fingerprint method is used to control the quality of the drug. It is sensitive, rapid, simple and accurate.
  • the chromatographic conditions of the drug preparation can be used to determine the quality of the drug.
  • the fingerprint of traditional Chinese medicine can be used in various stages of research and production process of traditional Chinese medicine preparations. It can be divided into the fingerprints of traditional Chinese medicines (raw materials), the fingerprints of traditional Chinese medicines (including decoction pieces and compatible particles) and the fingerprints of traditional Chinese medicine preparations. . If it is finer, it can also include fingerprints for intermediates in the process production process. It is worth noting that the establishment of chemical fingerprints of Chinese herbal medicines must take into account the influence of many factors.
  • the variety of traditional Chinese medicines, the historically formed foreign bodies of the same name, the synonyms of the same name, and the fluctuation of the same species in the environment of the place of origin and the components contained in different effective parts have brought many problems to the quality control.
  • the patent application number is 201210450660.0, and the invention name is: a preparation process of an antiviral traditional Chinese medicine composition and a method for measuring a fingerprint, and a method for determining a fingerprint of an extract of forsythia, rhubarb, ephedra, and houttuynia by ultrasonic countercurrent extraction is disclosed. Twenty-seven shared peaks were identified, and four of the common peaks were identified, namely, forsythiaside A, forsythin, rosin-4-O-glc, and rhein. The application does not disclose the technical contents of the present invention.
  • the invention requires A method for determining a high performance liquid chromatographic fingerprint of a traditional Chinese medicine composition, which comprises: forsythia, honeysuckle, radix isatidis, bitter almond, menthol, houttuynia, rhubarb, patchouli, cotton Ma Guanzhong, Rhodiola, Ephedra, Licorice, Gypsum, the fingerprinting method, calibrated 15 major chromatographic peaks, identified 9 of them, respectively, new chlorogenic acid, chlorogenic acid, crypto-acid, different Forsythiaside A, forsythiaside A, forsythin, quercetin, 4,5-di-O-caffeoylquinic acid, forsythin, glycyrrhizic acid, and this is not disclosed in the prior art.
  • Technical content comprises: forsythia, honeysuckle, radix isatidis, bitter almond, menthol, houttuynia, rhubar
  • the invention provides a method for determining a fingerprint of a traditional Chinese medicine composition.
  • a method for determining a fingerprint of a traditional Chinese medicine composition the raw materials of the traditional Chinese medicine composition include: forsythia, ephedra, rhubarb, houttuynia, honeysuckle, radix isatidis, patchouli, Mianma Guanzhong, Rhodiola, menthol , bitter almond, licorice, gypsum; the fingerprinting method comprises the following steps:
  • test solution taking the traditional Chinese medicine composition, and extracting with an organic solvent to obtain a test solution;
  • reference solution fresh chlorogenic acid, chlorogenic acid, cryptochlorogenic acid, isophoric acid A, forsythiaside A, forsythin, 4,5-di-O-caffeoylquinine
  • the acid, quercetin, ammonium glycyrrhizinate reference substance is dissolved in an organic solvent to obtain a reference solution;
  • Preparation of the reference solution take appropriate amount of forsythiaside A reference substance, add organic solvent to dissolve, to obtain a reference solution;
  • Chromatographic conditions a methanol-acetonitrile-phosphoric acid aqueous solution as a mobile phase, gradient elution, the concentration of the aqueous phosphoric acid solution is 0.1% to 0.5%;
  • Determination method separately draw the reference solution and the test solution, inject into the liquid chromatograph, and measure, that is, obtain.
  • the organic solvent comprises 50% to 100% methanol solution, 50% to 100% ethanol solution; further preferably, the organic solvent is 50% methanol.
  • the gradient elution procedure is:
  • the detection wavelength is 200-250 nm.
  • the traditional Chinese medicine composition is prepared from the following raw materials by weight: Forsythia 200-300, Ephedra 60-100, Rhubarb 40-60, Houttuynia 200-300, Honeysuckle 200-300, Ban GmbH 200-300, Patchouli 60-100, Mianma Guanzhong 200-300, Rhodiola 60-100, menthol 5-9, bitter almond 60-100, licorice 60-100, gypsum 200-300, fingerprinting method is as follows:
  • test solution Take 0.1-0.5g of the traditional Chinese medicine composition, accurately weigh it, place it in a conical flask, precisely add 40-100% methanol 10-50mL, weigh the weight, sonicate for 20-40 minutes , let cool, then weigh the weight, make up the lost weight with 40-100% methanol, shake, filter, take the filtrate, get it;
  • Preparation of reference solution weigh new chlorogenic acid, chlorogenic acid, cryptochlorogenic acid, isophoric acid A, forsythiaside A, forsythin, 4,5-di-O-coffee
  • the acyl quinic acid, quercetin, ammonium glycyrrhizinate reference substance was prepared to contain 18.4 ⁇ g/ml neochlorogenic acid, 27.2 ⁇ g/ml chlorogenic acid, 21.2 ⁇ g/ml cryptochlorogenic acid, and 27.2 ⁇ g/ml, respectively.
  • Forsythiaside A 21.6 ⁇ g/ml forsythiaside A, 16.0 ⁇ g/ml forsythin, 14.4 ⁇ g/ml 4,5-di-O-caffeoylquinic acid, 9.0 ⁇ g/ml quercetin, 24.4 ⁇ g/ml ammonium glycyrrhizinate mixed control solution is obtained;
  • Preparation of the reference solution Take the appropriate amount of forsythiaside A reference substance, accurately weighed, add 50% methanol to make a solution containing 101.2 ⁇ g per 1 ml, that is.
  • the column is a C 18 column; the detection wavelength is 239 nm, and the flow rate is 0.1-0.5 ml/min;
  • Determination method respectively, accurately draw the reference solution and the test solution each 1-10 ⁇ L, inject into the ultra-high liquid chromatograph, determine, record the chromatogram, that is.
  • the preferred pharmaceutical ingredients of the traditional Chinese medicine composition of the present invention are: Forsythia 200, Honeysuckle 300, Ban GmbH 200, Rhubarb 40, Patchouli 60, Mianma Guanzhong 300, Rhodiola 100, Menthol 9, Ephedra 60, bitter almond 100, houttuynia cordica 200, licorice 100, gypsum 200.
  • the preferred parts of the traditional Chinese medicine composition of the present invention are: Forsythia 300, Honeysuckle 200, Ban GmbH 300, Rhubarb 60, Patchouli 100, Mianma Guanzhong 200, Rhodiola 60, Menthol 5, Ephedra 100, bitter almond 60, houttuyniasis 300, licorice 60, gypsum 300.
  • the preferred pharmaceutical ingredients of the traditional Chinese medicine composition of the present invention are: Forsythia 278, Honeysuckle 294, Radix Isatidis 285, Rhubarb 55, Patchouli 95, Mianma Guanzhong 290, Rhodiola 87, Menthol 8.5, Ephedra 88, bitter almond 80, houttuynia 284, licorice 95, gypsum 277.
  • the preferred pharmaceutical ingredients of the traditional Chinese medicine composition of the present invention are: Forsythia 255, Honeysuckle 255, Radix Isatidis 255, Rhubarb 51, Patchouli 85, Mianma Guanzhong 255, Rhodiola 85, Menthol 7.5, Ephedra 85, bitter almond 85, houttuynia 255, licorice 85, gypsum 255.
  • the preparation method of the traditional Chinese medicine composition preparation of the invention is:
  • the method for determining the fingerprint of the present invention is preferably:
  • test solution Take 0.25g of the traditional Chinese medicine composition, accurately weigh it, place it in a conical flask, precisely add 50mL methanol 25mL, weigh the weight, sonication (power 500W, frequency 40kHz) for 30 minutes, Let cool, then weigh the weight, make up the lost weight with 50% methanol, shake it, filter it, take the filtrate, and get it;
  • Preparation of reference solution weigh new chlorogenic acid, chlorogenic acid, cryptochlorogenic acid, isophoric acid A, forsythiaside A, forsythin, 4,5-di-O-caffeoyl
  • the appropriate amount of linic acid, quercetin and ammonium glycyrrhizinate was prepared to contain 18.4 ⁇ g/ml neochlorogenic acid, 27.2 ⁇ g/ml chlorogenic acid, 21.2 ⁇ g/ml cryptochlorogenic acid, and 27.2 ⁇ g/ml.
  • Ester A 21.6 ⁇ g/ml forsythiaside A, 16.0 ⁇ g/ml forsythin, 14.4 ⁇ g/ml 4,5-di-O-caffeoylquinic acid, 9.0 ⁇ g/ml quercetin, 24.4 ⁇ g /ml ammonium glycyrrhizinate mixed control solution is obtained;
  • Preparation of the reference solution Take the appropriate amount of forsythiaside A reference substance, accurately weighed, add 50% methanol to make a solution containing 101.2 ⁇ g per 1 ml, that is.
  • the column was a Waters Acquity UPLC HSS T3 column with a column length of 100 mm, an inner diameter of 2.1 mm, a particle size of 1.8 ⁇ m, a detection wavelength of 239 nm, and a flow rate of 0.3 ml/min;
  • Assay method accurately draw 2 ⁇ L of each of the reference solution and the test solution, inject into an ultra-high liquid chromatograph, measure and record the chromatogram, that is, obtain.
  • the fingerprint mapping method of the present invention is also preferably:
  • test solution Take 0.1g of the traditional Chinese medicine composition, accurately weigh it, place it in a conical flask, add 50mL of methanol precisely, weigh the weight, sonicate for 40 minutes, let cool, then weigh the weight, use Make up the lost weight of methanol, shake it, filter it, take the filtrate, and get it;
  • Preparation of reference solution weigh new chlorogenic acid, chlorogenic acid, cryptochlorogenic acid, isophoric acid A, forsythiaside A, forsythin, 4,5-di-O-caffeoyl
  • the appropriate amount of linic acid, quercetin and ammonium glycyrrhizinate was prepared to contain 18.4 ⁇ g/ml neochlorogenic acid, 27.2 ⁇ g/ml chlorogenic acid, 21.2 ⁇ g/ml cryptochlorogenic acid, and 27.2 ⁇ g/ml.
  • Ester A 21.6 ⁇ g/ml forsythiaside A, 16.0 ⁇ g/ml forsythin, 14.4 ⁇ g/ml 4,5-di-O-caffeoylquinic acid, 9.0 ⁇ g/ml quercetin, 24.4 ⁇ g /ml ammonium glycyrrhizinate mixed control solution is obtained;
  • Preparation of the reference solution Take the appropriate amount of forsythiaside A reference substance, accurately weighed, add 50% methanol to make a solution containing 101.2 ⁇ g per 1 ml, that is.
  • the column was a Waters Acquity UPLC HSS T3 column; the detection wavelength was 239 nm, and the flow rate was 0.1 ml/min;
  • Determination method respectively, accurately draw 1 ⁇ L of the reference solution and the test solution, inject into the ultra-high liquid chromatograph, measure and record the chromatogram, that is, obtain.
  • the fingerprint mapping method of the present invention is also preferably:
  • test solution Take 0.5g of the traditional Chinese medicine composition, accurately weigh it, place it in a conical flask, accurately add 40mL methanol to 10mL, weigh the weight, sonicate for 20 minutes, let cool, then weigh the weight. , use 40% methanol to make up the lost weight, shake, filter, and take the filtrate to obtain;
  • Preparation of reference solution weigh new chlorogenic acid, chlorogenic acid, cryptochlorogenic acid, isophoric acid A, forsythiaside A, forsythin, 4,5-di-O-caffeoyl
  • the appropriate amount of linic acid, quercetin and ammonium glycyrrhizinate was prepared to contain 18.4 ⁇ g/ml neochlorogenic acid, 27.2 ⁇ g/ml chlorogenic acid, 21.2 ⁇ g/ml cryptochlorogenic acid, and 27.2 ⁇ g/ml.
  • Ester A 21.6 ⁇ g/ml forsythiaside A, 16.0 ⁇ g/ml forsythin, 14.4 ⁇ g/ml 4,5-di-O-caffeoylquinic acid, 9.0 ⁇ g/ml quercetin, 24.4 ⁇ g /ml ammonium glycyrrhizinate mixed control solution is obtained;
  • Preparation of the reference solution Take the appropriate amount of forsythiaside A reference substance, accurately weighed, add 50% methanol to make a solution containing 101.2 ⁇ g per 1 ml, that is.
  • the column was a column Waters XBridge TM Shield RP18; detection wavelength 239nm, flow rate 0.5ml / min;
  • Determination method respectively, accurately draw 1 ⁇ L of the reference solution and the test solution, inject into the ultra-high liquid chromatograph, measure and record the chromatogram, that is, obtain.
  • the fingerprint mapping method of the present invention is also preferably:
  • test solution Take 0.2g of the traditional Chinese medicine composition, accurately weigh it, place it in a conical flask, accurately add 20mL of methanol 20mL, weigh the weight, sonicate for 40 minutes, let cool, then weigh the weight , use 90% methanol to make up the lost weight, shake, filter, and take the filtrate to obtain;
  • Preparation of reference solution weigh new chlorogenic acid, chlorogenic acid, cryptochlorogenic acid, isophoric acid A, forsythiaside A, forsythin, 4,5-di-O-caffeoyl
  • the appropriate amount of linic acid, quercetin and ammonium glycyrrhizinate was prepared to contain 18.4 ⁇ g/ml neochlorogenic acid, 27.2 ⁇ g/ml chlorogenic acid, 21.2 ⁇ g/ml cryptochlorogenic acid, and 27.2 ⁇ g/ml.
  • Ester A 21.6 ⁇ g/ml forsythiaside A, 16.0 ⁇ g/ml forsythin, 14.4 ⁇ g/ml 4,5-di-O-caffeoylquinic acid, 9.0 ⁇ g/ml quercetin, 24.4 ⁇ g /ml ammonium glycyrrhizinate mixed control solution is obtained;
  • Preparation of the reference solution Take the appropriate amount of forsythiaside A reference substance, accurately weighed, add 50% methanol to make a solution containing 101.2 ⁇ g per 1 ml, that is.
  • the column was Phenomenex Kinetx XB-C18; the detection wavelength was 239 nm, and the flow rate was 0.4 ml/min;
  • Assay method accurately draw 6 ⁇ L of each of the reference solution and the test solution, inject into the ultra-high liquid chromatograph, measure and record the chromatogram, that is, obtain.
  • Example 1 Using the sample prepared in Example 1, the method for determining the fingerprint of the traditional Chinese medicine composition of the present invention was evaluated in various aspects, and the evaluation method was as follows:
  • the chromatograms of the test solutions at different detection wavelengths were examined at 210 nm to 400 nm, and the information amount at 239 nm was rich. Therefore, the detection wavelength was optimally selected to be 239 nm. For details, see the following Figures 1-4.
  • the ultrasonic and reflow methods were compared.
  • the number and intensity of the chromatographic peaks obtained by the ultrasonic and reflux extraction methods were not significantly different. For details, see Figure 12-13. Since the ultrasonic extraction method is simple, the ultrasound is selected. extract.
  • the reference solution and the test solution were injected separately, and 16 main chromatographic peaks were determined in the chromatogram of the test solution. According to the retention time and spectral characteristics of the chromatographic peak, it is determined that the peak of No. 2 in the fingerprint is new chlorogenic acid, the peak of No. 3 is chlorogenic acid, the peak of No. 5 is cryptochlorogenic acid, and the peak of No. 8 is isoprenoid A.
  • Peak 11 is forsythiaside A
  • peak 13 is quercetin
  • peak 14 is 4,5-di-O-caffeoylquinic acid
  • peak 15 is forsythin
  • peak 16 is glycyrrhizic acid.
  • test solution was taken and continuously injected 6 times according to the determined chromatographic conditions.
  • the RSD of each major peak area was less than 5%, indicating that the injection precision was good, and the results are shown in Table 2.
  • test solution was taken at 0h, 2h, 4h, 8h, 12h, 24h, and the RSD of each major peak area was less than 10%, indicating that the test solution was stable within 24h.
  • RSD of each major peak area was less than 10%, indicating that the test solution was stable within 24h.
  • Fingerprint establishment Take 10 batches of the sample of the traditional Chinese medicine composition of the invention, prepare the test solution according to the requirements of 3.4, and carry out the sample analysis under the above chromatographic conditions to obtain the fingerprints of 10 batches of the traditional Chinese medicine composition of the invention, and adopt The Chinese Medicine Chromatographic Fingerprint Similarity Evaluation System 2004A version developed by the National Pharmacopoeia Committee analyzed the fingerprints of 10 batches of samples. The median method was used to correct and automatically match the chromatographic peaks of each fingerprint to calculate the similarity. A comparison fingerprint was generated, as shown in Figure 29-30. The similarity results are shown in Table 5.
  • the invention establishes a liquid phase fingerprinting method of the traditional Chinese medicine composition of the invention, which has 16 chromatographic peaks, and 9 common peaks are identified by standard products: new chlorogenic acid (peak No. 2) and chlorogenic acid (peak No. 3). , cryptochlorogenic acid (peak 5), forsythine A (peak 8), forsythiaside A (peak 11), quercetin (peak 13), 4,5-di-O - caffeoyl quinic acid (peak 14), forsythin (peak 15), glycyrrhizic acid (peak 16).
  • the 11th chromatographic peak forsythiaside A with a stable peak and a large peak area was used as a reference peak, and the similarity of the fingerprints of the 10 batches of the traditional Chinese medicine composition of the present invention was high. It can be seen from the above experimental results that the method has good precision and stability, and repeatability, and provides a new method for improving the quality control of the traditional Chinese medicine composition.
  • Figure 1 Chromatogram of the test solution at a detection wavelength of 320 nm
  • FIG. 12 Chromatogram of the sample solution for 30 minutes of ultrasound
  • Figure 20 Comparison of the spectrum of the new chlorogenic acid in the reference solution and the test solution, the dotted line is the spectrum of the sample, and the solid line is the spectrum of the reference.
  • Figure 21 Comparison of the chlorogenic acid spectrum of the reference solution and the test solution, the dotted line is the spectrum of the sample, and the solid line is the spectrum of the reference.
  • Figure 22 Comparison of the spectrum of the cryptochlorogenic acid in the reference solution and the test solution, the dotted line is the spectrum of the sample, and the solid line is the spectrum of the reference.
  • Figure 23 Comparison of the reference solution of the reference solution with the testosterone A in the test solution, the dotted line is the spectrum of the test sample, and the solid line is the spectrum of the reference product.
  • Figure 24 Comparison of the reference solution of forsythiaside A in the reference solution and the test solution, the dotted line is the spectrum of the test sample, and the solid line is the spectrum of the reference product.
  • Figure 25 Comparison of the quercetin spectrum of the reference solution and the test solution, the dotted line is the spectrum of the sample, and the solid line is the spectrum of the reference.
  • Figure 26 Comparison of the spectrum of 4,5-di-O-caffeoylquinic acid in the reference solution and the test solution.
  • the dotted line is the spectrum of the sample, and the solid line is the spectrum of the reference.
  • Figure 27 Comparison of the forsythiaside spectra of the reference solution and the test solution, the dotted line is the spectrum of the test sample, and the solid line is the reference spectrum of the reference product.
  • Figure 28 Comparison of the spectrum of glycyrrhizic acid in the reference solution and the test solution, the dotted line is the spectrum of the sample, and the solid line is the spectrum of the reference.
  • Figure 33 Fingerprint of Example 3, wherein the peak 1 in Figure A is neochlorogenic acid, 2 is chlorogenic acid, 3 is cryptochlorogenic acid, 4 is isopenic acid ester A, and 5 is forsythiaside A. 6 is quercetin, 7 is 4,5-di-O-caffeoylquinic acid, 8 is forsythin, 9 is glycyrrhizic acid; B is a chromatogram of forsythiaside A reference solution; C is Fingerprint chromatogram of test solution
  • Figure 34 Fingerprint of Example 4, wherein the peak 1 in Figure A is new chlorogenic acid, 2 is chlorogenic acid, 3 is cryptochlorogenic acid, 4 is berberine A, and 5 is forsythiaside A. 6 is quercetin, 7 is 4,5-di-O-caffeoylquinic acid, 8 is forsythin, 9 is glycyrrhizic acid; B is a chromatogram of forsythiaside A reference solution; C is Fingerprint chromatogram of test solution
  • test solution Take 0.25g of the traditional Chinese medicine composition, accurately weigh it, place it in a conical flask, precisely add 50mL methanol 25mL, weigh the weight, ultrasonic power 500W, frequency 40kHz, ultrasonic 30 minutes, put Cold, weigh the weight again, make up the lost weight with 50% methanol, shake it, filter it, take the filtrate, and get it;
  • Preparation of reference solution weigh new chlorogenic acid, chlorogenic acid, cryptochlorogenic acid, isophoric acid A, forsythiaside A, forsythin, 4,5-di-O-caffeoyl
  • the appropriate amount of linic acid, quercetin and ammonium glycyrrhizinate was prepared to contain 18.4 ⁇ g/ml neochlorogenic acid, 27.2 ⁇ g/ml chlorogenic acid, 21.2 ⁇ g/ml cryptochlorogenic acid, and 27.2 ⁇ g/ml.
  • Ester A 21.6 ⁇ g/ml forsythiaside A, 16.0 ⁇ g/ml forsythin, 14.4 ⁇ g/ml 4,5-di-O-caffeoylquinic acid, 9.0 ⁇ g/ml quercetin, 24.4 ⁇ g /ml ammonium glycyrrhizinate mixed control solution is obtained;
  • Preparation of the reference solution Take the appropriate amount of forsythiaside A reference substance, accurately weighed, add 50% methanol to make a solution containing 101.2 ⁇ g per 1 ml, that is.
  • the column was a Waters Acquity UPLC HSS T3 column with a column length of 100 mm, an inner diameter of 2.1 mm, a particle size of 1.8 ⁇ m, a detection wavelength of 239 nm, and a flow rate of 0.3 ml/min;
  • Assay method accurately draw 2 ⁇ L of each of the reference solution and the test solution, inject into an ultra-high liquid chromatograph, measure and record the chromatogram, that is, obtain.
  • test solution Take 0.1g of the traditional Chinese medicine composition, accurately weigh it, place it in a conical flask, accurately add 50mL of methanol, 50mL, weigh the weight, sonicate for 40 minutes, let cool, then weigh the weight. , use 100% methanol to make up the lost weight, shake, filter, and take the filtrate to obtain;
  • Preparation of reference solution weigh new chlorogenic acid, chlorogenic acid, cryptochlorogenic acid, isophoric acid A, forsythiaside A, forsythin, 4,5-di-O-caffeoyl
  • the appropriate amount of linic acid, quercetin and ammonium glycyrrhizinate was prepared to contain 18.4 ⁇ g/ml neochlorogenic acid, 27.2 ⁇ g/ml chlorogenic acid, 21.2 ⁇ g/ml cryptochlorogenic acid, and 27.2 ⁇ g/ml.
  • Ester A 21.6 ⁇ g/ml forsythiaside A, 16.0 ⁇ g/ml forsythin, 14.4 ⁇ g/ml 4,5-di-O-caffeoylquinic acid, 9.0 ⁇ g/ml quercetin, 24.4 ⁇ g /ml ammonium glycyrrhizinate mixed control solution is obtained;
  • Preparation of the reference solution Take the appropriate amount of forsythiaside A reference substance, accurately weighed, add 50% methanol to make a solution containing 101.2 ⁇ g per 1 ml, that is.
  • the column was a Waters Acquity UPLC HSS T3 (column length 100 mm, inner diameter 2.1 mm, particle size 1.8 ⁇ m) column; detection wavelength 239 nm, flow rate 0.1 ml / min;
  • Determination method respectively, accurately draw 1 ⁇ L of the reference solution and the test solution, inject into the ultra-high liquid chromatograph, measure and record the chromatogram, that is, obtain.
  • the raw material formula is: 278g forsythia, 294g for honeysuckle, 285g for radix isatidis, 55g for rhubarb, 95g for patchouli, 290g for cotton horse, and 87g for Rhodiola Menthol 8.5g, ephedra 88g, bitter almond 80g, houttuynia 284g, licorice 95g, gypsum 277g, extracted according to the following process:
  • test solution Take 0.5g of the traditional Chinese medicine composition, accurately weigh it, place it in a conical flask, accurately add 40mL methanol to 10mL, weigh the weight, sonicate for 20 minutes, let cool, then weigh the weight. , use 40% methanol to make up the lost weight, shake, filter, and take the filtrate to obtain;
  • Preparation of reference solution weigh new chlorogenic acid, chlorogenic acid, cryptochlorogenic acid, isophoric acid A, forsythiaside A, forsythin, 4,5-di-O-caffeoyl
  • the appropriate amount of linic acid, quercetin and ammonium glycyrrhizinate was prepared to contain 18.4 ⁇ g/ml neochlorogenic acid, 27.2 ⁇ g/ml chlorogenic acid, 21.2 ⁇ g/ml cryptochlorogenic acid, and 27.2 ⁇ g/ml.
  • Ester A 21.6 ⁇ g/ml forsythiaside A, 16.0 ⁇ g/ml forsythin, 14.4 ⁇ g/ml 4,5-di-O-caffeoylquinic acid, 9.0 ⁇ g/ml quercetin, 24.4 ⁇ g /ml ammonium glycyrrhizinate mixed control solution is obtained;
  • Preparation of the reference solution Take the appropriate amount of forsythiaside A reference substance, accurately weighed, add 50% methanol to make a solution containing 101.2 ⁇ g per 1 ml, that is.
  • the column was Waters XBridge TM Shield RP18 (5 ⁇ m, 4.6mm ⁇ 250mm) column; detection wavelength 239nm, flow rate 0.5ml / min;
  • Determination method respectively, accurately draw 1 ⁇ L of the reference solution and the test solution, inject into the ultra-high liquid chromatograph, measure and record the chromatogram, that is, obtain.
  • the raw material formula is: weighed according to the ratio: Forsythia 255g, Honeysuckle 255g, Radix Isatidis 255g, Rhubarb 51g, Patchouli 85g, Mianma Guanzhong 255g, Rhodiola 85g, Menthol 7.5g, Ephedra 85g, Bitter Almond 85g Houttuynia 255g, licorice 85g, gypsum 255g, extracted according to the following process:
  • test solution Take 0.2g of the traditional Chinese medicine composition, accurately weigh it, place it in a conical flask, accurately add 20mL of methanol 20mL, weigh the weight, sonicate for 40 minutes, let cool, then weigh the weight , use 90% methanol to make up the lost weight, shake, filter, and take the filtrate to obtain;
  • Preparation of reference solution weigh new chlorogenic acid, chlorogenic acid, cryptochlorogenic acid, isophoric acid A, forsythiaside A, forsythin, 4,5-di-O-caffeoyl
  • the appropriate amount of linic acid, quercetin and ammonium glycyrrhizinate was prepared to contain 18.4 ⁇ g/ml neochlorogenic acid, 27.2 ⁇ g/ml chlorogenic acid, 21.2 ⁇ g/ml cryptochlorogenic acid, and 27.2 ⁇ g/ml.
  • Ester A 21.6 ⁇ g/ml forsythiaside A, 16.0 ⁇ g/ml forsythin, 14.4 ⁇ g/ml 4,5-di-O-caffeoylquinic acid, 9.0 ⁇ g/ml quercetin, 24.4 ⁇ g /ml ammonium glycyrrhizinate mixed control solution is obtained;
  • Preparation of the reference solution Take the appropriate amount of forsythiaside A reference substance, accurately weighed, add 50% methanol to make a solution containing 101.2 ⁇ g per 1 ml, that is.
  • the column was a Phenomenex Kinetx XB-C18 column; the detection wavelength was 239 nm, and the flow rate was 0.4 ml/min;
  • Assay method accurately draw 6 ⁇ L of each of the reference solution and the test solution, inject into the ultra-high liquid chromatograph, measure and record the chromatogram, that is, obtain.

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Abstract

L'invention concerne un procédé de détermination par chromatographique liquide haute performance de l'empreinte d'une composition de médecine chinoise. La composition de médecine chinoise est constituée de forsythia, de chèvrefeuille japonais, de racine d'indigowad, d'amande amère, de menthe-camphre, de houttuynia cordata, de rhubarbe, de patchouli, de rhizome de fougère mâle, de racine et de rhizome de kirilow rhodiola, d'éphédra, de réglisse et de gypse. Le procédé de détermination de l'empreinte détecte 15 pics chromatographiques principaux, et identifie neuf des pics chromatographiques principaux, à savoir l'acide néochlorogénique, l'acide chlorogénique, l'acide cryptochlorogénique, l'isoforsythiaside A, la forsythiaside A, la forsythine, la quercitrine, l'acide 4,5-di-O-caféoylquinique et le sel d'ammonium d'acide glycyrrhizique.
PCT/CN2017/075513 2016-03-03 2017-03-03 Procédé de détermination de l'empreinte d'une composition de médecine chinoise WO2017148426A1 (fr)

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CN115494173A (zh) * 2022-09-21 2022-12-20 江西济民可信药业有限公司 一种小儿热咳口服液指纹图谱检测方法

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101683421A (zh) * 2008-09-26 2010-03-31 北京以岭药业有限公司 一种中药组合物在制备治疗反复呼吸道感染药物中的应用
CN103800523A (zh) * 2012-11-13 2014-05-21 河北以岭医药研究院有限公司 一种抗病毒中药组合物的制备方法及指纹图谱的测定方法
CN104345111A (zh) * 2013-08-08 2015-02-11 河北以岭医药研究院有限公司 一种中药组合物制剂中多种活性成分含量的测定方法

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1194752C (zh) * 2003-07-01 2005-03-30 河北以岭医药研究院有限公司 一种抗病毒中药组合物及制备方法

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101683421A (zh) * 2008-09-26 2010-03-31 北京以岭药业有限公司 一种中药组合物在制备治疗反复呼吸道感染药物中的应用
CN103800523A (zh) * 2012-11-13 2014-05-21 河北以岭医药研究院有限公司 一种抗病毒中药组合物的制备方法及指纹图谱的测定方法
CN104345111A (zh) * 2013-08-08 2015-02-11 河北以岭医药研究院有限公司 一种中药组合物制剂中多种活性成分含量的测定方法

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
"Liquorice", PHARMACOPOEIA OF THE PEOPLE'S REPUBLIC OF CHINA, vol. I, 30 June 2015 (2015-06-30), pages 86 - 87 *
CHEN, KUIXIA ET AL.: "Simultaneous Quantification of Ten Phenolic Acids in Lonicerae Japonicae Flos by HPLC-DAD", JOURNAL OF CHINESE PHARMACEUTICAL SCIENCES, vol. 22, no. 6, 31 December 2013 (2013-12-31), pages 521 - 526 *
LUO, BIN ET AL.: "Study on Chemical Constituents of Extract of Forsythia Suspense", CHINESE JOURNAL OF EXPERIMENTAL TRADITIONAL MEDICAL FORMULAE, vol. 19, no. 3, 28 February 2013 (2013-02-28), pages 143 - 146 *
SHEN, JUAN ET AL.: "Determination of Organic Acids and Glycosides in Different Species of Lonicerae Flos", CHINESE HERBAL MEDICINES, vol. 45, no. 20, 31 December 2014 (2014-12-31), pages 2993 - 2996 *
YU , JIANPING ET AL.: "Quantitative Determination of Quercitrin in Houttuynia Cordata by High Performance Liquid Chromatography", HERALD OF MEDICINE, vol. 27, no. 3, 31 March 2008 (2008-03-31), pages 331 *
ZHANG, YONGFENG ET AL.: "Rapid Determination of Ingredients in Water Extract of Lianhua Qingwen Capsule by UPLC", CHINESE JOURNAL OF MODERN APPLIED PHARMACY, vol. 31, no. 1, 31 January 2014 (2014-01-31), pages 78 - 80, XP055414053 *

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* Cited by examiner, † Cited by third party
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