WO2018184527A1 - 植物萃取物在制备用于提升tgm1、krt、aqp3、flg、gba、及has基因表达的组合物中的用途 - Google Patents

植物萃取物在制备用于提升tgm1、krt、aqp3、flg、gba、及has基因表达的组合物中的用途 Download PDF

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WO2018184527A1
WO2018184527A1 PCT/CN2018/081720 CN2018081720W WO2018184527A1 WO 2018184527 A1 WO2018184527 A1 WO 2018184527A1 CN 2018081720 W CN2018081720 W CN 2018081720W WO 2018184527 A1 WO2018184527 A1 WO 2018184527A1
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Prior art keywords
extract
gene
tea extract
keratin
composition
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PCT/CN2018/081720
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English (en)
French (fr)
Inventor
林咏翔
陈怡卉
甘恺雯
刘复诚
陈巧婷
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大江生医股份有限公司
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Application filed by 大江生医股份有限公司 filed Critical 大江生医股份有限公司
Priority to ES18780313T priority Critical patent/ES2924075T3/es
Priority to CN201880021296.1A priority patent/CN110573171A/zh
Priority to CN202310720393.2A priority patent/CN116585399A/zh
Priority to US16/499,436 priority patent/US10918682B2/en
Priority to EP18780313.5A priority patent/EP3607961B1/en
Publication of WO2018184527A1 publication Critical patent/WO2018184527A1/zh

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Definitions

  • the present invention relates to a composition containing a plant extract, in particular to a method for promoting transglutaminase 1 gene, keratin gene, aquaporin 3 gene, polykeratin microfilament gene, glucocerebrosidase Use of a plant extract-containing composition for expression of a gene, and a hyaluronic acid synthase gene.
  • the epidermis layer is the outermost layer of the skin.
  • the stratum corneum, the granular layer, the spinous layer and the basal layer are sequentially arranged from the outside to the inside.
  • the epidermal layer is mainly formed by the differentiation of undifferentiated cylindrical keratinocytes in the basal layer. The process is called keratinization.
  • the water content in keratinocytes is high. As the cells metabolize and differentiate, the shape of keratinocytes will gradually become flat, and the nucleus and organelles will begin to degenerate and shrink, and dead cells with no nucleus and organelles will be formed in the stratum corneum.
  • the main function of the epidermis is to keep the skin water-retaining and form a skin barrier to resist various external injuries.
  • the outermost layer of the epidermis is composed of a weakly acidic sebum film and a stratum corneum such as a brick wall structure, which can lock the skin.
  • the moisture and oil, the invasion of skin surface bacteria, and the resistance to foreign objects and ultraviolet light, have a very important protective effect on the human body.
  • the keratinocytes are dead cells, the main component is keratin, keratin can absorb moisture to keep the skin moist, and keratinocytes secrete substances such as hyaluronic acid as interstitial cells.
  • keratinocytes secrete substances such as hyaluronic acid as interstitial cells.
  • keratinocytes can not maintain normal metabolic cycle, and the skin's epidermal barrier is damaged, leaving the skin rough, dry and desquamate, vulnerable and vulnerable. Stimulating, sensitive redness and decreased skin water retention capacity, so the health of the stratum corneum is very important for skin moisturization and resistance to external injuries.
  • the ingredients or drugs currently used for this purpose are limited. If it is a certain plant extract, it must be administered at a relatively high dose, which has limitations in terms of cost of use and effect. Therefore, if multiple extracts can be administered When a small amount of components can produce a high dose effect of a single component, the use of the aforementioned effects can not only greatly increase, but also reduce the cost of use.
  • a plant extract for the preparation of a transglutaminase 1 (TGM1) gene, a keratin (KRT) gene, and aquaporin 3 (Aquaporin 3, Use of a composition of AQP3) gene, Filaggrin (FLG) gene, Glucocerebrosidase (GBA) gene, and Hyaluronan synthase (HAS) gene expression, wherein the plant extracts
  • the composition comprises at least one selected from the group consisting of Pu'er tea extract and blueberry extract, Pu'er tea extract and spinach extract, four season spring tea extract and blueberry extract, and four season spring tea extract and spinach extract. combination.
  • the keratin gene comprises a keratin 1 (KRT1) gene, a keratin 10 (Keratin 10, KRT10) gene, and a keratin 14 (Kartatin 14, KRT14) gene; the hyaluronic acid synthase gene
  • the Hyaluronan synthase 2 (HAS2) gene and the Hyaluronan synthase 3 (HAS3) gene are included.
  • the plant extract comprises at least 0.125 mg/mL of Pu'er tea extract and at least 0.125 mg/mL of spinach extract, at least 0.125 mg/mL of four season spring tea extract and at least 0.125 mg /mL of spinach extract, at least 0.25 mg/mL of Pu'er tea extract and at least 0.25 mg/mL of blueberry extract, or at least 0.25 mg/mL of Pu'er tea extract and at least 0.25 mg/mL of four seasons spring tea extract Things.
  • the composition has the effect of promoting skin moisturization.
  • the composition further comprises a pharmaceutically acceptable carrier, and the composition is in the form of a powder, granule, liquid, gel or paste, and the composition is prepared into a pharmaceutical or The form of food products.
  • Figure 1 is a moisturizing fraction of each of the plant extract compositions of the present invention.
  • the preparation of the Four Seasons Spring tea extract is as follows: First, the leaves of the Four Seasons Spring tea plant are washed and dried, and the four seasons spring tea leaves are coarsely crushed by a pulverizer. Then, the crude material of the four seasons spring tea leaves is extracted with water as a solvent, and the solvent is uniformly mixed with the coarse and fine grains of the four seasons spring tea leaves at a liquid-solid ratio of 5-20:1 to 5, and the extraction temperature is 50 ° C to 100 ° C.
  • the extraction time is about 0.5 ⁇ 3 hours
  • the crude extract of the four seasons spring tea is obtained
  • the crude extract is cooled to room temperature, filtered through a 400 mesh filter to remove residual solids, Further, it is concentrated under reduced pressure at 45 ° C to 70 ° C to obtain a concentrated product, which is the four season spring tea extract of the present invention.
  • the spinach extract is obtained by extracting spinach (Spinacia oleracea), and the spinach extract used in this example can be purchased from Hongxiang Agricultural Processing Factory.
  • the blueberry extract is obtained by extracting the fruit of North American blueberry (Vaccinium Cyanococcus), and the blueberry extract used in this embodiment can be purchased from Yuanhong Herbal Research Co., Ltd.
  • the Puerh tea extract is obtained by extracting post-fermented leaves of Camellia sinensis.
  • the Pu'er tea extract used in this example can be purchased from Nanjing Zelang Biotechnology Co., Ltd.
  • Example 2 The plant extract composition of the invention enhances the expression of TGM1, KRT1, KRT10, KRT14, APQ3 and FLG genes
  • human primary epidermal keratinocytes are used for gene analysis of TGM1, KRT1, KRT10, KRT14, APQ3 and FLG.
  • the human primary skin keratinocytes were purchased from CELLnTEC (Switzerland) No. HPEK-50, and the cells were cultured in serum-free keratinocyte-SFM (Gibco, Inc., #10724-011, USA).
  • RNAs in the two sets of keratinocytes were collected using an RNA extraction reagent kit (purchased from Geneaid, Taiwan, Lot No. FC24015-G), and then utilized. III reverse transcriptase (available from Invitrogene, USA, No.
  • RNA 2000ng cDNA product as a template and primers for reverse transcription to produce mRNA
  • ABI StepOnePlus TM Real-Time PCR system (Thermo Fisher Scientific, USA) and KAPA FAST qPCR Kits (purchased from Sigma, USA, number 38220000000) were used to quantify the real-time reverse transcription polymerase chain reaction by combining the six sets of reverse transcription products with the combined primers of Table 1.
  • the test quantifies the mRNA expression levels of TGM1 gene, KRT1 gene, KRT10 gene, KRT14 gene, APQ3 gene, FLG gene, GBA gene, HAS2 gene and HAS3 gene under the conditions of 95 ° C for 1 second and 60 ° C for 20 seconds.
  • the moisturizing fraction of each of the plant extracting compositions of the present invention is shown in Table 2 and Figure 1.
  • TGM transglutaminase1
  • KRT Keratin
  • FGF Filaggrin
  • AQP Aquaporin
  • Hyaluronan synthase HAS
  • HAS Hyaluronan synthase
  • GAA Glucocerebrosidase
  • the moisturizing fractions obtained by treating keratinocytes with 0.125 mg/mL Pu'er tea extract, four season spring tea extract, and spinach extract alone were 2.16, 4.2, and 2.04, respectively; however, they were mixed at a ratio of 0.125 mg/mL in a ratio of 1:1.
  • the composition of the Pu'er tea extract and the spinach extract, and the composition of the four season spring tea extract and the spinach extract are 7.2 and 10.2, respectively; 0.25 mg/mL Pu'er tea extract, four season spring tea extract,
  • the hydrating fraction obtained by treating the keratinocytes with blueberry extract is 3.5, 3.8, and 4.7, respectively; however, the combination of Pu'er tea extract and blueberry extract mixed with 1:1 ratio of 0.25 mg/mL, and the four season spring tea
  • the composition of the extract and the blueberry extract are 7.4 and 6.6, respectively.
  • the composition of the Pu'er tea extract and the blueberry extract, the combination of the Pu'er tea extract and the spinach extract, and the four season spring tea extract and the The composition of the blueberry extract, and the combination of the four season spring tea extract and the spinach extract have a remarkable effect on enhancing the skin moisturizing gene expression, respectively, compared with the Pu'er tea extract, the four season spring tea extract, and the spine.
  • the extract and blueberry extract can more effectively enhance the expression of TGM1, KRT1, KRT10, KRT14, APQ3 and FLG genes, and can more effectively maintain the keratinocyte arrangement of the skin, make the stratum corneum structure intact, and effectively enhance the skin.
  • the composition of the Pu'er tea extract and the blueberry extract of the present invention the combination of the Pu'er tea extract and the spinach extract, the composition of the four season spring tea extract and the blueberry extract, or the four season spring tea extract
  • the combination of the substance and the spinach extract can produce an unexpected multiplication effect, so that the expression of the skin moisturizing gene is significantly increased compared with the simple Puer tea extract or the four season spring tea extract, and the skin keratinocyte arrangement can be more effectively maintained. Makes the structure of the stratum corneum intact and enhances the skin barrier function and causes the skin to form more moisturizing factors.
  • the plant for extracting of the present invention for enhancing expression of transglutaminase 1 gene, keratin gene, aquaporin 3 gene, polykeratin microfilament gene, glucocerebrosidase gene, and hyaluronic acid synthase gene
  • the use of the composition of the substance may be further added to a food, a health food, a dietary supplement or a drink, and the composition of the plant extract of the present invention may also be used for preparing a pharmaceutical composition, and the pharmaceutical composition may also be used. Further, carriers or other adjuvants well known in the art may be added, and the pharmaceutical compositions may be, but are not limited to, solutions, capsules, or lozenges.

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Abstract

植物萃取物在制备用于提升TGM1、KRT、AQP3、FLG、GBA、及HAS基因表达的组合物中的用途，所述植物萃取物包含选自由普洱茶萃取物与蓝莓萃取物、普洱茶萃取物与菠菜萃取物、四季春茶萃取物与蓝莓萃取物、及四季春茶萃取物与菠菜萃取物所组成的组。相较于单一成分的萃取物，本申请的萃取物组合能够提升皮肤保湿基因的表达。

Description

植物萃取物在制备用于提升TGM1、KRT、AQP3、FLG、GBA、及HAS基因表达的组合物中的用途
相关申请的交叉引用
本申请主张在2017年04月03日在美国提交的美国专利申请号No.62/480,860、以及在2017年05月08日在美国提交的美国专利申请号No.62/503,185的优先权，其全部内容通过引用包含于此。
技术领域
本发明涉及一种含有植物萃取物的组合物，尤其涉及一种用以提升转谷氨酰胺酶1基因、角蛋白基因、水通道蛋白3基因、聚角蛋白微丝基因、葡萄糖脑苷酯酶基因、及玻尿酸合成酶基因表达的含有植物萃取物的组合物的用途。
背景技术
表皮层为皮肤的最外层，由外往内依序为角质层、颗粒层、有棘层及基底层，表皮层主要由基底层中未分化的圆柱型角质细胞持续向上进行分化形成，此过程称为角质化。角质细胞内含水量高，随着细胞向上代谢分化，角质细胞形状会逐渐变成扁平状，且细胞核及细胞器开始退化萎缩，并在角质层形成不具细胞核与细胞器的死细胞。表皮层的主要功能为使皮肤保水，并形成皮肤屏障以抵御各种外来伤害，其中表皮层最外层由弱酸性的皮脂膜以及如砖墙结构的角质层所构成，此屏障能锁住皮肤的水分和油脂、抵抗皮肤表面病菌入侵，及对抗外界异物及紫外光等伤害，对人体有非常重要的保护作用。
表皮层中的角质层，其角质细胞虽然为死细胞，但其主要成分为角蛋白(keratin)，角蛋白能吸收水分使皮肤保持湿润，角质细胞也会分泌如玻尿酸等物质作为细胞间质，以维持表皮层皮肤屏障的结构完整，以防止皮肤水分散失及形成完整防护。当皮肤接触过冷或过热的环境以及照射紫外光等刺激，会导致角质细胞无法维持正常的代谢循环，并使得皮肤表皮层屏障受损，而 让皮肤变得粗糙、干燥脱屑、脆弱易受刺激、敏感泛红且会使皮肤保水能力下降，因此角质层的健康对于皮肤保湿以及抵御外来伤害着实非常重要。
综合上面所述，为了改善因角质细胞受损导致皮肤变得脆弱、易敏，以及皮肤保水能力下降的问题，开发一种能有效维持角质细胞排列、维持角质层结构完整以及使角质细胞分泌更多保湿因子，以提升皮肤屏障以及保湿功能的组合物，着实有其必要性。
然而，目前用于该用途的成分或药物有限，若为某种植物萃取物，其必须施予相当高的剂量，在使用成本上以及效果上有其局限，因此，若能施予多种萃取物的少量组分，即可产生单一成分的高剂量效果时，对于前述功效的运用，不但可大幅提升，也同时降低了使用成本。
发明内容
缘此，本发明的一目的在提供一种植物萃取物用于制备提升转谷氨酰胺酶1(Transglutaminase 1,TGM1)基因、角蛋白(Keratin,KRT)基因、水通道蛋白3(Aquaporin 3,AQP3)基因、聚角蛋白微丝(Filaggrin,FLG)基因、葡萄糖脑苷酯酶(Glucocerebrosidase,GBA)基因、及玻尿酸合成酶(Hyaluronan synthase,HAS)基因表达的组合物的用途，其中该植物萃取物包含选自由普洱茶萃取物与蓝莓萃取物、普洱茶萃取物与菠菜萃取物、四季春茶萃取物与蓝莓萃取物、及四季春茶萃取物与菠菜萃取物所组成的组中至少任一组合。
在本发明的一实施例中，其中该角蛋白基因包含角蛋白1(Keratin1,KRT1)基因、角蛋白10(Keratin10,KRT10)基因及角蛋白14(Keratin14,KRT14)基因；该玻尿酸合成酶基因包含玻尿酸合成酶2(Hyaluronan synthase 2,HAS2)基因及玻尿酸合成酶3(Hyaluronan synthase 3,HAS3)基因。
在本发明的一实施例中，该植物萃取物包含至少0.125mg/mL的普洱茶萃取物及至少0.125mg/mL的菠菜萃取物、至少0.125mg/mL的四季春茶萃取物及至少0.125mg/mL的菠菜萃取物、至少0.25mg/mL的普洱茶萃取物及至少0.25mg/mL的蓝莓萃取物、或至少0.25mg/mL的普洱茶萃取物及至少0.25mg/mL的四季春茶萃取物。
在本发明的一实施例中，该组合物具有促进皮肤保湿的功效。
在本发明的一实施例中，该组合物进一步包含医药上可接受的载体，且该组合物为粉末、颗粒、液体、胶状或膏状的剂型，又该组合物被制备成医药品或食品产品的形式。
附图说明
图1为本发明的各植物萃取组合物的保湿分数。
具体实施方式
以下将配合附图进一步说明本发明的实施方式，下述所列举的实施例用以阐明本发明的发明特点及应用，而非以限定本发明的范围，对于本领域技术人员来说，在不脱离本发明的精神和范围内，还可以做出若干改进和润饰，因此本发明的保护范围应当以本申请权利要求书的内容为准。
实施例1植物萃取物的制备
实施例1-1本发明四季春茶萃取物的制备方法
四季春茶(Four Seasons Spring tea)萃取物的制备方法如下：首先，将四季春茶茶叶(leaves of Four Seasons Spring tea plant)洗净并进行干燥，再用粉碎机将四季春茶茶叶进行粗碎，接着以水为溶剂对四季春茶茶叶的粗碎物进行萃取，该溶剂与四季春茶茶叶粗碎物以液固比5～20：1～5均匀混合，萃取温度为50℃～100℃，优选为75℃～95℃，萃取时间约为0.5～3小时，得到四季春茶的粗萃取物，将该粗萃取物冷却至室温后，经由400目的滤网过滤以移除残余固体物，再进一步于45℃～70℃进行减压浓缩而获得浓缩产物，即为本发明的四季春茶萃取物。
实施例1-2本发明菠菜萃取物的制备方法
菠菜萃取物通过对菠菜(Spinacia oleracea)进行萃取而获得，本实施例中使用的菠菜萃取物可购自弘祥农产加工厂。
实施例1-3本发明蓝莓萃取物的制备方法
蓝莓萃取物通过对北美蓝莓(Vaccinium Cyanococcus)果实进行萃取而获得，本实施例中使用的蓝莓萃取物可购自元弘草本科研有限公司。
实施例1-4本发明普洱茶萃取物的制备方法
普洱茶(Puerh tea)萃取物通过对普洱茶茶叶(post-fermented leaves of Camellia sinensis)进行萃取而获得，本实施例中使用的普洱茶萃取物可购自南京泽朗生物科技有限公司。
实施例2本发明植物萃取组合物提升TGM1、KRT1、KRT10、KRT14、APQ3及FLG基因表达的效果
本发明以人类初代皮肤角质细胞(human primary epidermal keratinocytes,HPEK)进行TGM1、KRT1、KRT10、KRT14、APQ3及FLG的基因分析。该人类初代皮肤角质细胞购自CELLnTEC公司(瑞士)编号HPEK-50，将该细胞在无血清的角质细胞培养液(keratinocyte-SFM)(Gibco公司，编号为#10724-011，美国)中培养。
首先，将1.5×10 5个人类初代皮肤角质细胞种植于含2mL的上述培养液的6孔培养板中，将人类初代角质细胞分成十组各别加入：(1)0.125mg/mL的普洱茶萃取物、(2)0.125mg/mL的菠菜萃取物、(3)0.125mg/mL的四季春茶萃取物、(4)0.125mg/mL的以1:1比例混合的普洱茶萃取物与菠菜萃取物的组合物、(5)0.125mg/mL的以1:1比例混合的四季春茶萃取物与菠菜萃取物的组合物、(6)0.25mg/mL的普洱茶萃取物、(7)0.25mg/mL的蓝莓萃取物、(8)0.25mg/mL的四季春茶萃取物、(9)0.25mg/mL的以1:1比例混合的普洱茶萃取物与蓝莓萃取物的组合物、(10)0.25mg/mL的以1:1比例混合的四季春茶萃取物与蓝莓萃取物的组合物，并以没有加入任何萃取物的组别为空白对照组。加入植物萃取物后置于37℃培养箱，使角质细胞形成受植物萃取物刺激后的角质细胞，于第6小时与24小时更换培养液，培养液为角质细胞培养液及植物萃取液。接着，将角质细胞加入裂解液(lysis buffer)收集成管后，使用RNA萃取试剂试剂盒(购自Geneaid公司，台湾，Lot No.FC24015-G)分别收集两组角质细胞内的RNA，接着利用
Figure PCTCN2018081720-appb-000001
III反转录酶(购自Invitrogene公司，美国，编号18080-051)以2000ng的萃取RNA为模板并以引物产生mRNA反转录的相应cDNA产物，接着利用ABI StepOnePlus TM Real-Time PCR system(Thermo Fisher Scientific公司，美国)以及KAPA
Figure PCTCN2018081720-appb-000002
FAST qPCR Kits(购自Sigma公司，美国，编号38220000000)将六组反转录后产物分别以表1的组合引物进行定量实时反转录聚合酶链式反应(quantitative  real-time reverse transcription polymerase chain reaction)试验，定量TGM1基因、KRT1基因、KRT10基因、KRT14基因、APQ3基因、FLG基因、GBA基因、HAS2基因及HAS3基因的mRNA表达量，条件为95℃反应1秒，60℃反应20秒，总共40个循环，其中定量数值取自阈值循环数(Ct)，而目标基因的mRNA相对量推导自方程式2 -△Ct，其中△Ct＝Ct 目标基因-Ct ACTB(β-肌动蛋白，beta-actin)，接着以SCORE法来定量该十组中个别基因表达量的分数，再将各基因的表达分数求和作为该组的保湿分数，其中SCORE法是利用ACTB基因及基准基因(空白对照组中相应的个别基因)的循环阈值来计算。
表1定量实时反转录聚合酶连锁反应的组合引物
Figure PCTCN2018081720-appb-000003
Figure PCTCN2018081720-appb-000004
本发明的各植物萃取组合物的保湿分数如表2及图1所示，保湿分数越高代表保湿能力越高。先前研究指出转谷氨酰胺酶(Transglutaminase1,TGM)会使角质化细胞的细胞膜与结构蛋白间形成强力键结，并能增加表皮层的强度与稳定性；角蛋白(Keratin,KRT)会形成角蛋白微丝，而聚角蛋白微丝(Filaggrin,FLG)会帮助角蛋白微丝组装成坚固的网络，为皮肤提供强度和弹性；水通道蛋白(Aquaporin,AQP)则会增加角质细胞内水的通透性，以提高角质细胞的含水量；玻尿酸合成酶(Hyaluronan synthase,HAS)会促进角质细胞分泌玻尿酸的能力，并使皮肤角质层结构完整且提升皮肤屏障功能，能使皮肤保水力提升；而葡萄糖脑苷酯酶(Glucocerebrosidase,GBA)则与维持角质结构完整有关。单独以0.125mg/mL普洱茶萃取物、四季春茶萃取物、及菠菜萃取物处理角质细胞所获得的保湿分数分别为2.16、4.2、及2.04；然而以0.125mg/mL的1:1比例混合的普洱茶萃取物与菠菜萃取物的组合物、及四季春茶萃取物与菠菜萃取物的组合物则分别为7.2及10.2；单独以0.25mg/mL普洱茶萃取物、四季春茶萃取物、及蓝莓萃取物处理角质细胞所获得的保湿分数分别为3.5、3.8、及4.7；然而以0.25mg/mL的1:1比例混合的普洱茶萃取物与蓝莓萃取物的组合物、及四季春茶萃取物与蓝莓萃取物的组合物则分别为7.4及6.6，由以上结果可知普洱茶萃取物与蓝莓萃取物的组合物、普洱茶萃取物与菠菜萃取物的组合物、四季春茶萃取物与蓝莓萃取物的组合物、及四季春茶萃取物与菠菜萃取物的组合物对于提升皮肤保湿基因表达的效果相当显著，皆较单独普洱茶萃取物、四季春茶萃取物、菠菜萃取物及蓝莓萃取物更为有效地提升TGM1、KRT1、KRT10、KRT14、APQ3及FLG基因的表达，并能更有效的维持皮肤角质细胞排列，使皮肤角质层结构完整，也能有效地提升皮肤屏障功能以及使皮肤形成更多保湿因子。
表2各组萃取物的保湿分数
Figure PCTCN2018081720-appb-000005
Figure PCTCN2018081720-appb-000006
综上所述，本发明的普洱茶萃取物与蓝莓萃取物的组合物、普洱茶萃取物与菠菜萃取物的组合物、四季春茶萃取物与蓝莓萃取物的组合物、或四季春茶萃取物与菠菜萃取物的组合物能够产生超乎预期的相乘效果，使皮肤保湿基因的表达较单纯普洱茶萃取物或四季春茶萃取物显著增加，并能更有效地维持皮肤角质细胞排列，使皮肤角质层结构完整，并提升皮肤屏障功能以及使皮肤形成更多保湿因子。
因此，本发明的用于提升转谷氨酰胺酶1基因、角蛋白基因、水通道蛋白3基因、聚角蛋白微丝基因、葡萄糖脑苷酯酶基因、及玻尿酸合成酶基因表达的含有植物萃取物的组合物的用途，也可进一步添加于食品、保健食品、膳食补充品或饮品中，且也可将本发明的植物萃取物的组合物用于制备医药组合物，该医药组合物也可进一步加入所属技术领域所熟知的载体或其他辅剂，该医药组合物的剂型，可为但不限于溶液、胶囊、或锭剂。

Claims (11)

  1. 植物萃取物在制备提升转谷氨酰胺酶1(Transglutaminase 1,TGM1)基因、角蛋白(Keratin,KRT)基因、水通道蛋白3(Aquaporin 3,AQP3)基因、聚角蛋白微丝(Filaggrin,FLG)基因、葡萄糖脑苷酯酶(Glucocerebrosidase,GBA)基因、及玻尿酸合成酶(Hyaluronan synthase,HAS)基因表达的组合物中的用途，其中所述植物萃取物包含选自由普洱茶萃取物与蓝莓萃取物、普洱茶萃取物与菠菜萃取物、四季春茶萃取物与蓝莓萃取物、及四季春茶萃取物与菠菜萃取物所组成的组中的至少任一组合。
  2. 如权利要求1所述的用途，其中所述角蛋白(KRT)基因包含角蛋白1(Keratin1,KRT1)基因、角蛋白10(Keratin10,KRT10)基因及角蛋白14(Keratin14,KRT14)基因。
  3. 如权利要求1所述的用途，其中所述玻尿酸合成酶(HAS)基因包含玻尿酸合成酶2(Hyaluronan synthase 2,HAS2)基因及玻尿酸合成酶3(Hyaluronan synthase 3,HAS3)基因。
  4. 如权利要求1所述的用途，其中所述植物萃取物包含至少0.125mg/mL的普洱茶萃取物及至少0.125mg/mL的菠菜萃取物。
  5. 如权利要求1所述的用途，其中所述植物萃取物包含至少0.125mg/mL的四季春茶萃取物及至少0.125mg/mL的菠菜萃取物。
  6. 如权利要求1所述的用途，其中所述植物萃取物包含至少0.25mg/mL的普洱茶萃取物及至少0.25mg/mL的蓝莓萃取物。
  7. 如权利要求1所述的用途，其中所述植物萃取物包含至少0.25mg/mL的普洱茶萃取物及至少0.25mg/mL的四季春茶萃取物。
  8. 如权利要求1所述的用途，其中所述组合物具有促进皮肤保湿的功效。
  9. 如权利要求1所述的用途，其中所述组合物进一步包含医药上可接受的载体。
  10. 如权利要求1所述的用途，其中所述组合物为粉末、颗粒、液体、胶状或膏状的剂型。
  11. 如权利要求1所述的用途，其中所述组合物被制备成医药品或食品 产品的形式。
PCT/CN2018/081720 2017-04-03 2018-04-03 植物萃取物在制备用于提升tgm1、krt、aqp3、flg、gba、及has基因表达的组合物中的用途 WO2018184527A1 (zh)

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