JPH0438394B2 - - Google Patents

Info

Publication number
JPH0438394B2
JPH0438394B2 JP63212641A JP21264188A JPH0438394B2 JP H0438394 B2 JPH0438394 B2 JP H0438394B2 JP 63212641 A JP63212641 A JP 63212641A JP 21264188 A JP21264188 A JP 21264188A JP H0438394 B2 JPH0438394 B2 JP H0438394B2
Authority
JP
Japan
Prior art keywords
lipase
recombinant
promoter
humicola
sequence
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
JP63212641A
Other languages
English (en)
Japanese (ja)
Other versions
JPH01157383A (ja
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from DK450087A external-priority patent/DK450087D0/da
Priority claimed from DK656087A external-priority patent/DK656087D0/da
Priority claimed from DK205488A external-priority patent/DK205488D0/da
Application filed filed Critical
Publication of JPH01157383A publication Critical patent/JPH01157383A/ja
Publication of JPH0438394B2 publication Critical patent/JPH0438394B2/ja
Granted legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C12N9/2414—Alpha-amylase (3.2.1.1.)
    • C12N9/2417—Alpha-amylase (3.2.1.1.) from microbiological source
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18—Carboxylic ester hydrolases (3.1.1)
    • C12N9/20—Triglyceride splitting, e.g. by means of lipase
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Mycology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
JP63212641A 1987-08-28 1988-08-29 組換え体ヒュミコーラリパーゼおよびその調製方法 Granted JPH01157383A (ja)

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
DK450087A DK450087D0 (da) 1987-08-28 1987-08-28 Fremgangsmaade til fremstilling af proteiner
DK4500/87 1987-08-28
DK656087A DK656087D0 (da) 1987-12-15 1987-12-15 Enzym
DK6560/87 1987-12-15
DK205488A DK205488D0 (da) 1988-04-15 1988-04-15 Enzym
DK2054/88 1988-04-15

Publications (2)

Publication Number Publication Date
JPH01157383A JPH01157383A (ja) 1989-06-20
JPH0438394B2 true JPH0438394B2 (enExample) 1992-06-24

Family

ID=27221490

Family Applications (1)

Application Number Title Priority Date Filing Date
JP63212641A Granted JPH01157383A (ja) 1987-08-28 1988-08-29 組換え体ヒュミコーラリパーゼおよびその調製方法

Country Status (5)

Country Link
EP (1) EP0305216B1 (enExample)
JP (1) JPH01157383A (enExample)
AT (1) ATE125865T1 (enExample)
DE (1) DE3854249T2 (enExample)
ES (1) ES2076939T3 (enExample)

Families Citing this family (831)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5278066A (en) * 1985-08-09 1994-01-11 Gist-Brocades Nv Molecular cloning and expression of gene encoding lipolytic enzyme
US5536661A (en) * 1987-03-10 1996-07-16 Novo Nordisk A/S Process for the production of protein products in aspergillus
US5766912A (en) * 1986-03-17 1998-06-16 Novo Nordisk A/S Humicola lipase produced in aspergillus
US5830735A (en) * 1987-03-06 1998-11-03 Gist-Brocades Nv Method for producing lipolytic enzymes using transformed Pseudomonas
GB8810954D0 (en) * 1988-05-09 1988-06-15 Unilever Plc Enzymatic detergent & bleaching composition
US5292448A (en) * 1988-05-10 1994-03-08 Lever Brothers Company, Division Of Conopco, Inc. Enzymatic detergent composition
GB8915658D0 (en) * 1989-07-07 1989-08-23 Unilever Plc Enzymes,their production and use
US5658871A (en) * 1989-07-07 1997-08-19 Lever Brothers Company, Division Of Conopco, Inc. Microbial lipase muteins and detergent compositions comprising same
DK173590D0 (da) * 1990-06-06 1990-07-19 Novo Nordisk As Rekombinante terapeutiske lipaser
BR9106839A (pt) * 1990-09-13 1993-07-20 Novo Nordisk As Variante de lipase,construcao de dna,vetor de expressao de recombinante,celula,planta,processo para produzir uma variante de lipase,aditivo e composicao de detergente
US5869438A (en) * 1990-09-13 1999-02-09 Novo Nordisk A/S Lipase variants
DK46693D0 (enExample) * 1993-04-23 1993-04-23 Novo Nordisk As
US5892013A (en) * 1990-09-13 1999-04-06 Novo Nordisk A/S Lipase variants
ATE176498T1 (de) * 1991-04-02 1999-02-15 Novo Nordisk As Xylanase, entsprechende rekombinante dna-sequenz, xylanase enthaltendes agens, und seine verwendung
FI934812A7 (fi) 1991-05-01 1993-10-29 Novozymes As Stabiloidut entsyymit
WO1993011254A1 (en) * 1991-11-26 1993-06-10 Novo Nordisk A/S Protease-stable proteins
DK88892D0 (da) * 1992-07-06 1992-07-06 Novo Nordisk As Forbindelse
DK104592D0 (da) * 1992-08-21 1992-08-21 Novo Nordisk As Fremgangsmaade
DK154292D0 (da) * 1992-12-23 1992-12-23 Novo Nordisk As Nyt enzym
DK39693D0 (da) † 1993-04-02 1993-04-02 Novo Nordisk As Enzym
DK47493D0 (da) * 1993-04-27 1993-04-27 Novo Nordisk As Fremgangsmaade til fremstilling af ost
DE4329463A1 (de) * 1993-09-01 1995-03-02 Cognis Bio Umwelt Mehrenzymgranulate
CA2178007A1 (en) * 1993-12-01 1995-06-08 Randy M. Berka Aspergillus expression system
CA2178008A1 (en) * 1993-12-01 1995-06-08 Randy M. Berka Aspergillus foetidus expression system
CA2203398A1 (en) * 1994-10-26 1996-05-09 Thomas Sandal An enzyme with lipolytic activity
US5714373A (en) * 1995-02-27 1998-02-03 Recombinant Biocatalysis, Inc. Thermococcus AV4 and enzymes produced by the same
DE19515072A1 (de) * 1995-04-28 1996-10-31 Cognis Bio Umwelt Cellulasehaltiges Waschmittel
US5646025A (en) * 1995-05-05 1997-07-08 Novo Nordisk A/S Scytalidium catalase gene
US6936289B2 (en) 1995-06-07 2005-08-30 Danisco A/S Method of improving the properties of a flour dough, a flour dough improving composition and improved food products
JP3039685B2 (ja) 1995-06-07 2000-05-08 ダニスコ エイ／エス 穀粉軟塊の特性を改良する方法、穀粉軟塊を改良する組成物、および改良された食品
EP0851913B1 (en) * 1995-08-11 2004-05-19 Novozymes A/S Novel lipolytic enzymes
BE1009650A5 (fr) * 1995-10-12 1997-06-03 Genencor Int Systeme d'expression, vecteur et cellule transformee par ce vecteur.
JP3792725B2 (ja) * 1996-01-19 2006-07-05 ノボザイムス，インコーポレイティド 糸状菌の形態変異体
CN100432099C (zh) 1996-07-05 2008-11-12 诺沃奇梅兹有限公司 转录因子
AU4772697A (en) 1996-11-04 1998-05-29 Novo Nordisk A/S Subtilase variants and compositions
BR9712878A (pt) 1996-11-04 2000-02-01 Novo Nordisk As Variante de enzima subtilase, processos para a identificação de uma variante de protease apresentando estabilidade autoproteolìtica e paraq a produção de uma enzima subtilase mutante e de uma variante de subtilase, sequência de dna, vetor, célula hospedeira microbiana, composição e uso de uma variante de subtilase.
ATE280221T1 (de) 1997-04-09 2004-11-15 Danisco Verwendung von lipase zur verbesserung von teigen und backwaren
ATE293359T1 (de) 1997-12-22 2005-05-15 Novozymes As Kohlenhydratoxidase sowie verwendung derselben beim backen
AU3247699A (en) 1998-02-17 1999-09-06 Novo Nordisk A/S Lipase variant
US6156552A (en) * 1998-02-18 2000-12-05 Novo Nordisk A/S Lipase variants
DE69904941T3 (de) 1998-07-21 2008-01-31 Danisco A/S Lebensmittel
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DE69943346D1 (de) 1998-12-18 2011-05-19 Novozymes As Subtilase Enzyme der I-S1- und I-S2-Untergruppen mit einem zusätzlichen Aminosäurerest in einer aktiven Schleifenregion
AU4025700A (en) 1999-03-22 2000-10-09 Novo Nordisk Biotech, Inc. Promoters for expressing genes in a fungal cell
CN100455663C (zh) 1999-03-31 2009-01-28 诺维信公司 脂肪酶变体
CN100523181C (zh) 1999-03-31 2009-08-05 诺维信公司 具有碱性α-淀粉酶活性的多肽及其编码核酸
WO2000060063A1 (en) 1999-03-31 2000-10-12 Novozymes A/S Lipase variant
CN1234854C (zh) 1999-03-31 2006-01-04 诺维信公司 具有碱性α－淀粉酶活性的多肽以及编码该多肽的核酸
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WO2000071685A1 (en) 1999-05-20 2000-11-30 Novozymes A/S Subtilase enzymes of the i-s1 and i-s2 sub-groups having at least one additional amino acid residue between positions 132 and 133
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ATE407201T1 (de) 1999-05-20 2008-09-15 Novozymes As Subtilase-enzyme der i-s1 und i-s2 untergruppen mit wenigstens einem zusätzlichen aminosäurerest zwischen positionen 126 und 127
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