MX364987B - Alfa-amilasas variantes de bacillus subtilis y metodos de uso de las mismas. - Google Patents

Alfa-amilasas variantes de bacillus subtilis y metodos de uso de las mismas.

Info

Publication number
MX364987B
MX364987B MX2014005185A MX2014005185A MX364987B MX 364987 B MX364987 B MX 364987B MX 2014005185 A MX2014005185 A MX 2014005185A MX 2014005185 A MX2014005185 A MX 2014005185A MX 364987 B MX364987 B MX 364987B
Authority
MX
Mexico
Prior art keywords
methods
amylases
bacillus subtilis
variant alpha
amye
Prior art date
Application number
MX2014005185A
Other languages
English (en)
Inventor
A Estell David
A Cuevas William
Lee Sang-Kyu
D Power Scott
W Ramer Sandra
R Toppozada Amr
Wallace Louise
Shaw Andrew
Chin Regina
J Pepsin Michael
A Requadt Carol
Original Assignee
Danisco Us Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Danisco Us Inc filed Critical Danisco Us Inc
Publication of MX364987B publication Critical patent/MX364987B/es

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C12N9/2414—Alpha-amylase (3.2.1.1.)
    • C12N9/2417—Alpha-amylase (3.2.1.1.) from microbiological source
    • A—HUMAN NECESSITIES
    • A21—BAKING; EDIBLE DOUGHS
    • A21D—TREATMENT OF FLOUR OR DOUGH FOR BAKING, e.g. BY ADDITION OF MATERIALS; BAKING; BAKERY PRODUCTS
    • A21D8/00—Methods for preparing or baking dough
    • A21D8/02—Methods for preparing dough; Treating dough prior to baking
    • A21D8/04—Methods for preparing dough; Treating dough prior to baking treating dough with microorganisms or enzymes
    • A21D8/042—Methods for preparing dough; Treating dough prior to baking treating dough with microorganisms or enzymes with enzymes
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16—Organic compounds
    • C11D3/38—Products with no well-defined composition, e.g. natural products
    • C11D3/386—Preparations containing enzymes, e.g. protease or amylase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/02—Monosaccharides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/14—Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase, e.g. by cellulase, hemicellulase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/06—Ethanol, i.e. non-beverage
    • C12P7/14—Multiple stages of fermentation; Multiple types of microorganisms or re-use of microorganisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01001—Alpha-amylase (3.2.1.1)
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N11/00—Investigating flow properties of materials, e.g. viscosity, plasticity; Analysing materials by determining flow properties
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Food Science & Technology (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Detergent Compositions (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Bakery Products And Manufacturing Methods Therefor (AREA)

Abstract

La presente invención se refiere a una variante aislada de polipéptido, caracterizada porque tiene actividad de a-amilasa y por lo menos una característica alterada que mejora el desempeño de la enzima, en donde la característica alterada se selecciona del grupo que consiste de reducción incrementada de la viscosidad, actividad amilolítica incrementada, termoestabilidad incrementada, estabilidad incrementada ante el pH, y remoción incrementada de machas, caracterizada porque comprende: i) un polipéptido que tiene una secuencia de aminoácidos por lo menos 90% idéntica a la SEQ ID NO: 1 y una modificación en una o más posiciones que se seleccionan del grupo que consiste de 9, 68, 88, 126, 139, 158, 192, 214 y 257 de la secuencia de aminoácidos de la SEQ ID NO: 1; o ii) un polipéptido que tiene una secuencia de aminoácidos por lo menos 92% idéntica a la SEQ ID NO: 2, y una modificación en una o más posiciones que se seleccionan del grupo que consiste de 9, 68, 88, 126, 139,158, 192, 214 y 257 de la secuencia de aminoácidos de la SEQ ID NO: 2, en donde la modificación produce una variante de polipéptido que tiene un índice de desempeño (PI) mayor de 1.0 para al menos una característica alterada que mejora el desempeño de la enzima.
MX2014005185A 2008-06-06 2009-06-05 Alfa-amilasas variantes de bacillus subtilis y metodos de uso de las mismas. MX364987B (es)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US5951308P 2008-06-06 2008-06-06
US5961808P 2008-06-06 2008-06-06
PCT/US2009/046506 WO2009149419A2 (en) 2008-06-06 2009-06-05 Variant alpha-amylases from bacillus subtilis and methods of use, thereof

Publications (1)

Publication Number Publication Date
MX364987B true MX364987B (es) 2019-05-17

Family

ID=41217791

Family Applications (4)

Application Number Title Priority Date Filing Date
MX2010013123A MX2010013123A (es) 2008-06-06 2009-06-05 Alfa-amilasas variantes de bacillus subtilis y metodos de uso de las mismas.
MX2013014962A MX350760B (es) 2008-06-06 2009-06-05 Alfa-amilasas variantes de bacillus subtilis y metodos de uso de las mismas.
MX2014005185A MX364987B (es) 2008-06-06 2009-06-05 Alfa-amilasas variantes de bacillus subtilis y metodos de uso de las mismas.
MX2010013114A MX2010013114A (es) 2008-06-06 2009-06-05 Alfa-amilasas variantes de bacillus subtilis y metodos de uso de las mismas.

Family Applications Before (2)

Application Number Title Priority Date Filing Date
MX2010013123A MX2010013123A (es) 2008-06-06 2009-06-05 Alfa-amilasas variantes de bacillus subtilis y metodos de uso de las mismas.
MX2013014962A MX350760B (es) 2008-06-06 2009-06-05 Alfa-amilasas variantes de bacillus subtilis y metodos de uso de las mismas.

Family Applications After (1)

Application Number Title Priority Date Filing Date
MX2010013114A MX2010013114A (es) 2008-06-06 2009-06-05 Alfa-amilasas variantes de bacillus subtilis y metodos de uso de las mismas.

Country Status (12)

Country Link
US (4) US8323945B2 (es)
EP (2) EP2297312B1 (es)
JP (3) JP2011522546A (es)
CN (3) CN102112605B (es)
BR (2) BRPI0913367A2 (es)
CA (2) CA2726803A1 (es)
DK (2) DK2297312T3 (es)
ES (1) ES2538406T3 (es)
HU (1) HUE025251T2 (es)
MX (4) MX2010013123A (es)
PL (1) PL2297313T3 (es)
WO (2) WO2009149395A2 (es)

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US8323945B2 (en) 2012-12-04
JP2011522546A (ja) 2011-08-04
WO2009149395A3 (en) 2010-03-25
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BRPI0915606A2 (pt) 2021-03-09
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CN102112605A (zh) 2011-06-29
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CN103773749A (zh) 2014-05-07
US20130149770A1 (en) 2013-06-13
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BRPI0913367A2 (pt) 2015-08-04
CN102131924B (zh) 2017-06-09
PL2297313T3 (pl) 2015-08-31
JP2014168469A (ja) 2014-09-18
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US20160010129A1 (en) 2016-01-14
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