MX2010013114A - Alfa-amilasas variantes de bacillus subtilis y metodos de uso de las mismas. - Google Patents

Alfa-amilasas variantes de bacillus subtilis y metodos de uso de las mismas.

Info

Publication number
MX2010013114A
MX2010013114A MX2010013114A MX2010013114A MX2010013114A MX 2010013114 A MX2010013114 A MX 2010013114A MX 2010013114 A MX2010013114 A MX 2010013114A MX 2010013114 A MX2010013114 A MX 2010013114A MX 2010013114 A MX2010013114 A MX 2010013114A
Authority
MX
Mexico
Prior art keywords
methods
amylases
bacillus subtilis
use
variant alpha
Prior art date
Application number
MX2010013114A
Other languages
English (en)
Inventor
David A Estell
William A Cuevas
Michael J Pepsin
Sandra W Ramer
Louise Wallace
Sang-Kyu Lee
Scott D Power
Andrew Shaw
Amr R Toppozada
Regina Chin
Carol A Requadt
Original Assignee
Danisco Us Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority to US5951308P priority Critical
Priority to US5961808P priority
Application filed by Danisco Us Inc filed Critical Danisco Us Inc
Priority to PCT/US2009/046506 priority patent/WO2009149419A2/en
Publication of MX2010013114A publication Critical patent/MX2010013114A/es

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/24Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405Glucanases
    • C12N9/2408Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411Amylases
    • C12N9/2414Alpha-amylase (3.2.1.1.)
    • C12N9/2417Alpha-amylase (3.2.1.1.) from microbiological source
    • AHUMAN NECESSITIES
    • A21BAKING; EDIBLE DOUGHS
    • A21DTREATMENT, e.g. PRESERVATION, OF FLOUR OR DOUGH, e.g. BY ADDITION OF MATERIALS; BAKING; BAKERY PRODUCTS; PRESERVATION THEREOF
    • A21D8/00Methods for preparing or baking dough
    • A21D8/02Methods for preparing dough; Treating dough prior to baking
    • A21D8/04Methods for preparing dough; Treating dough prior to baking treating dough with microorganisms or enzymes
    • A21D8/042Methods for preparing dough; Treating dough prior to baking treating dough with microorganisms or enzymes with enzymes
    • CCHEMISTRY; METALLURGY
    • C11ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11DDETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16Organic compounds
    • C11D3/38Products with no well-defined composition, e.g. natural products
    • C11D3/386Preparations containing enzymes, e.g. protease, amylase
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/24Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405Glucanases
    • C12N9/2408Glucanases acting on alpha -1,4-glucosidic bonds
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00Preparation of compounds containing saccharide radicals
    • C12P19/02Monosaccharides
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00Preparation of compounds containing saccharide radicals
    • C12P19/14Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase, e.g. by cellulase, hemicellulase
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00Preparation of oxygen-containing organic compounds
    • C12P7/02Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/06Ethanol, i.e. non-beverage
    • C12P7/14Multiple stages of fermentation; Multiple types of microorganisms or re-use of microorganisms
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y302/00Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01001Alpha-amylase (3.2.1.1)
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N11/00Investigating flow properties of materials, e.g. viscosity, plasticity; Analysing materials by determining flow properties
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02EREDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00Technologies for the production of fuel of non-fossil origin
    • Y02E50/10Biofuels
    • Y02E50/17Grain bio-ethanol

Abstract

Se proporcionan alfa-arnilasas de Bacillus subtilis (AmyE), variantes de las mismas, ácidos nucleicos que codifican las mismas, y células hospederas que comprenden los ácidos nucleicos. Se describen métodos para usar AmyE o variantes del mismo, incluyendo licuefacción y/o sacarificación de almidón. Tales métodos pueden proporcionar azúcares útiles para producción de etanol o producción de jarabe de almidón de alta fructosa. En algunos casos, las amilasas se pueden usar en un bajo pH, en la ausencia de calcio, y/o en la ausencia de una glucoamilasa.
MX2010013114A 2008-06-06 2009-06-05 Alfa-amilasas variantes de bacillus subtilis y metodos de uso de las mismas. MX2010013114A (es)

Priority Applications (3)

Application Number Priority Date Filing Date Title
US5951308P true 2008-06-06 2008-06-06
US5961808P true 2008-06-06 2008-06-06
PCT/US2009/046506 WO2009149419A2 (en) 2008-06-06 2009-06-05 Variant alpha-amylases from bacillus subtilis and methods of use, thereof

Publications (1)

Publication Number Publication Date
MX2010013114A true MX2010013114A (es) 2011-04-05

Family

ID=41217791

Family Applications (4)

Application Number Title Priority Date Filing Date
MX2010013114A MX2010013114A (es) 2008-06-06 2009-06-05 Alfa-amilasas variantes de bacillus subtilis y metodos de uso de las mismas.
MX2014005185A MX364987B (es) 2008-06-06 2009-06-05 Alfa-amilasas variantes de bacillus subtilis y metodos de uso de las mismas.
MX2010013123A MX2010013123A (es) 2008-06-06 2009-06-05 Alfa-amilasas variantes de bacillus subtilis y metodos de uso de las mismas.
MX2013014962A MX350760B (es) 2008-06-06 2009-06-05 Alfa-amilasas variantes de bacillus subtilis y metodos de uso de las mismas.

Family Applications After (3)

Application Number Title Priority Date Filing Date
MX2014005185A MX364987B (es) 2008-06-06 2009-06-05 Alfa-amilasas variantes de bacillus subtilis y metodos de uso de las mismas.
MX2010013123A MX2010013123A (es) 2008-06-06 2009-06-05 Alfa-amilasas variantes de bacillus subtilis y metodos de uso de las mismas.
MX2013014962A MX350760B (es) 2008-06-06 2009-06-05 Alfa-amilasas variantes de bacillus subtilis y metodos de uso de las mismas.

Country Status (12)

Country Link
US (4) US9090887B2 (es)
EP (2) EP2297313B1 (es)
JP (3) JP2011522546A (es)
CN (3) CN102131924B (es)
BR (1) BRPI0913367A2 (es)
CA (2) CA2726274C (es)
DK (2) DK2297312T3 (es)
ES (1) ES2538406T3 (es)
HU (1) HUE025251T2 (es)
MX (4) MX2010013114A (es)
PL (1) PL2297313T3 (es)
WO (2) WO2009149395A2 (es)

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