JPS6474992A - Dna sequence, plasmid and production of lipase - Google Patents

Dna sequence, plasmid and production of lipase

Info

Publication number
JPS6474992A
JPS6474992A JP23353187A JP23353187A JPS6474992A JP S6474992 A JPS6474992 A JP S6474992A JP 23353187 A JP23353187 A JP 23353187A JP 23353187 A JP23353187 A JP 23353187A JP S6474992 A JPS6474992 A JP S6474992A
Authority
JP
Japan
Prior art keywords
lipase
plasmid
escherichia coli
dna
dna sequence
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP23353187A
Inventor
Wataru Kugimiya
Yasuo Otani
Yukio Hashimoto
Original Assignee
Fuji Oil Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Fuji Oil Co Ltd filed Critical Fuji Oil Co Ltd
Priority to JP23353187A priority Critical patent/JPS6474992A/en
Publication of JPS6474992A publication Critical patent/JPS6474992A/en
Application status is Pending legal-status Critical

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/16Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18Carboxylic ester hydrolases (3.1.1)
    • C12N9/20Triglyceride splitting, e.g. by means of lipase

Abstract

PURPOSE:To efficiently produce lipase, by inserting a structural gene of 1,3-position specific lipase of a bacterium of genus Pseudomonas and a structural gene of thermostable lipase of a bacterium belonging to the genus Bacillus into a multicopy plasmid and transducing Escherichia coli. CONSTITUTION:Chromosome DNA of Pseudomonas fragi IFO3458 strain is digested with restriction enzyme Sau3A, linked with pUC9 cleft by BamHI and T ligase, Escherichia coli JM83 is transduced and pHB-1.0 containing DNA sequence corresponding to amino acid sequence shown by the formula (A is Ileu; X is His; Y is Gln; B is Ala) is obtained from JM83 (pKK-O). DNA sequence corresponding to an amino acid sequence shown by the formula (A is Ala; X is Leu; Y is Leu; B is Gly) is also contained in plasmid PHS-6 having DNA fragment of HindIII-SaI derived from chromosome DNA of Bacillus stearothermophilus and Escherichia coli transduced with the plasmid is cultivated in a medium to give lipase.
JP23353187A 1987-09-16 1987-09-16 Dna sequence, plasmid and production of lipase Pending JPS6474992A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP23353187A JPS6474992A (en) 1987-09-16 1987-09-16 Dna sequence, plasmid and production of lipase

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP23353187A JPS6474992A (en) 1987-09-16 1987-09-16 Dna sequence, plasmid and production of lipase

Publications (1)

Publication Number Publication Date
JPS6474992A true JPS6474992A (en) 1989-03-20

Family

ID=16956504

Family Applications (1)

Application Number Title Priority Date Filing Date
JP23353187A Pending JPS6474992A (en) 1987-09-16 1987-09-16 Dna sequence, plasmid and production of lipase

Country Status (1)

Country Link
JP (1) JPS6474992A (en)

Cited By (316)

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