CN1108457A - 用于洗涤剂领域的新的脂酶变异体 - Google Patents

用于洗涤剂领域的新的脂酶变异体 Download PDF

Info

Publication number
CN1108457A
CN1108457A CN94190243A CN94190243A CN1108457A CN 1108457 A CN1108457 A CN 1108457A CN 94190243 A CN94190243 A CN 94190243A CN 94190243 A CN94190243 A CN 94190243A CN 1108457 A CN1108457 A CN 1108457A
Authority
CN
China
Prior art keywords
lipase
pseudomonas
modification
enzyme
wild
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN94190243A
Other languages
English (en)
Inventor
J·M·范·德·兰
H·B·M·伦廷
L·J·S·M·马伦纳斯
M·M·J·考克斯
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Danisco US Inc
Original Assignee
Gist Brocades NV
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Gist Brocades NV filed Critical Gist Brocades NV
Publication of CN1108457A publication Critical patent/CN1108457A/zh
Pending legal-status Critical Current

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18—Carboxylic ester hydrolases (3.1.1)
    • C12N9/20—Triglyceride splitting, e.g. by means of lipase
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16—Organic compounds
    • C11D3/38—Products with no well-defined composition, e.g. natural products
    • C11D3/386—Preparations containing enzymes, e.g. protease or amylase
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16—Organic compounds
    • C11D3/38—Products with no well-defined composition, e.g. natural products
    • C11D3/386—Preparations containing enzymes, e.g. protease or amylase
    • C11D3/38627—Preparations containing enzymes, e.g. protease or amylase containing lipase

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Detergent Compositions (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

本发明公开了修饰的脂酶，其中，至少相当于野 生型类产碱假单胞菌脂酶21位上的甲硫氨酸被另 一种氨基酸取代，该脂酶表现出所需性质的改变，特 别是改善了洗涤性能。优选的实施方案中，所述甲硫 氨酸由亮氨酸、丝氨酸或丙氨酸取代。

Description

用于洗涤剂领域的新的脂酶变异体
本发明涉及修饰的脂酶及其在例如洗涤剂或清洗组合物中的用途。
脂酶是能够水解脂类的酶，它们被广泛用于如脂肪和油类加工、洗涤剂、诊断试剂等领域。
许多微生物生产细胞外脂酶(三酰基甘油酰基水解酶，E.C.3.1.1.3)。已在美国专利No.3,950,277中公开了适宜的脂酶。这些脂酶是从各种微生物如假单胞菌属、曲霉菌属、肺炎球菌属、葡萄球菌属、结核分枝杆菌、解脂芽生圆酵母、和核盘菌属中得到的。
在如EP463100(产碱假单胞菌)、EP0218272(类产碱假单胞菌)、EP0214761(洋葱假单胞菌)、EP0258068(Thermomyces)，EP206390(Pseccdomonas chromobacter、荧光假单胞杆菌、菌实假单胞菌、还原氮假单胞菌、划界假单胞菌和Chromobacter viscosum)中，给出了脂酶在洗涤剂组合物中用途的实例。
对于所需的应用领域，假单胞菌脂酶似乎具有良好的特征。因此，已经用假单胞菌属菌种来得到脂酶。为了在发酵中提高脂酶的产率，在同源和异源的宿主菌株中，已克隆并表达了几种脂酶基因。已经报道过的，从中克隆脂酶基因的假单胞杆菌属种的实例是洋葱假单胞菌(EP331376)、Pseudomonas glumae(EP464922)、类产碱假单胞菌(EP334462)、莓实假单胞菌(EP318775)。
假单胞杆菌属种的分类是以Palleroni等人(Int J.Syst.Bacteiol.23：333(1973))报道的，DNA-rRNA和DNA-DNA杂交研究为基础的。在Bergey′s Manual of Systematic Bacteriology(Vol.l，Seetion4，PP.160-161(1984)，Eds N.R.Kriegand J.G.Hoet，Williams and Wilkins，Baltimor/rondon)中，可见到更详细的综述。该综述同时报道的形态学数据和16s核糖体RNA同源性也支持该种分类法。
可以将具有良好应用特征的所述假单胞单菌菌株分成两个DNA同源组：类产碱假单胞菌、产碱假单胞菌、铜绿色假单胞杆菌(参见，如EP0334462)、施氏假单胞菌和门多萨假单胞菌非常相似，属于I类。例如铜绿色假单胞杆菌脂酶与类产碱假单胞菌脂酶有81％的同源性。Pseudomonas glumae和洋葱假单胞菌属于II类。表1列出了得自不同菌株的脂酶基因的同源性比较。从这种同源性比较中，可以看出，得自莓实假单胞菌的脂酶介于I类和II类之间。
来源于I类和II类的脂酶间有许多差异。发现属于I类假单胞菌的脂酶比属于II类的那些有更高的疏水性，因此是更亲脂的。这种特性对用于洗涤剂中是非常有益的。类产碱假单胞菌脂酶的疏水性使其容易粘附于表面。其它类或生物如Humicola Lanuginosa的脂酶常具有更高的亲水性。I类高疏水性脂酶仅需适当处理即可用于洗涤剂。
                                       表1
                          与其它假单胞菌脂酶的同源性
  No.     菌种     1     2     3     4     5
  1   类产碱假单胞菌     100     71     40     38     41
  2   铜绿假单胞菌     81     100     37     41     36
  3   洋葱假单胞菌     52     41     100     33     78
  4   莓实假单胞菌     56     51     46     100     34
  5   P.glumae     59     41     82     52     100
表的下部分是核酸序列的比较。上部分是氨基酸序列的比较。
可以用已提到的脂酶作为洗涤剂组合物中的组分。除脂酶外，根据其配方，洗涤剂组合物还可含有许多已知组分。
——洗涤剂粉末一般含有助洗剂(如沸石，磷酸盐)，表面活性剂(阴离子的，非离子的)聚合物(如，丙烯酰聚合物)，漂白剂前体(如硼酸盐)，漂白激活剂，结构剂(Structurant)(如硅酸盐)。调整pH的化合物(如，碱)。
——液体洗涤剂一般含有表面活性剂(如，阴离子的和非离子的)，漂白剂前体(如，硼酸盐)，漂白激活剂，调整pH的化合物(如，碱)。
也可将其它组分如酶(如，蛋白酶，淀粉酶)，有机酸，无机盐，纤维软化剂加到上述组合物中。
为了可在洗涤剂组合物中作为一种组分使用，除洗涤特性外，脂酶还应对所述组合物中的其它组分具有耐受性。
可以经若干途径，如经典的筛选方式或现代遗传和蛋白质工程技术，研制或发现用于洗涤剂组合物的酶。
对具有所需酶活性的生物或微生物的筛选可以通过如下完成，例如，从微生物或所述微生物的培养上清液中分离并纯化所述酶，确定其生化特性，检测这些生化特性是否符合特定用途的需要。如果不能从其天然的产生菌中得到所鉴定的酶，则可用重组DNA技术分离编码酶的基因，在另一种生物中表达该基因，分离并纯化所表达的酶，然后检测其是否适于所预期的用途。
另一种获得具有预期用途的新酶的方法是修饰现存的酶。特别是可经化学修饰方法(参见WO91/16423)达到该目的。通常，这些方法是非特异性的，也就是说：它们可修饰带一般侧链的所有可接触的残基，或它们依赖于所修饰的适当氨基酸的存在，而经常不能修饰难以达到的氨基酸，除非酶分子是未折叠的。
然而，通过诱变编码基因进行的酶修饰则没有上述的非特异性问题。可以用随机诱变或定点诱变完成诱变。
通过用化学诱变剂或放射诱变处理整个微生物而进行的随机诱变当然产生修饰过的酶。在这种情况下，必须要有极有效的选择方法以筛选这些特定、稀少的突变体。也可以作到提高经随机诱变分离突变体酶的可能性，即在克隆编码基因后，在体外或体内对其进行诱变，并在适当的宿主细胞内，通过再克隆突变基因表达所编码的酶。用于生产修饰酶的适当宿主有，例如，细菌(大肠杆菌、芽胞杆菌，假单胞菌)，酵母或真菌(曲霉菌属)。这种情况下，必须要有适宜的生物学选择方法，以选择所需的突变体酶。这些生物学选择方法不必直接选择酶，但最好适于工业化应用。例如，EP0407225描述了一种脂酶，它具有改善了的抗蛋白酶或氧化剂作用的稳定性。事实上，这些改进可以解决特定的问题，但不一定会产生适宜的，甚至更好的洗涤性能(参见，如EP0328229)。
EP0407225还公开了在Pseudomonas glumae脂酶序列中的突变。但该申请未说明：对于编码来源于其它微生物的脂酶的基因，应取代哪个残基。通常，对于一种特定酶的最佳突变选择并不是另一种同源酶自身最好的突变选择，而对于相关程度更低的酶当然更不是。
WO92/05249描述了带有不同疏水性或静电特性的脂类接触区的脂酶突变体，它可以产生改善的洗涤性能、稳定性、贮存稳定性以及特异性。但，该申请中描述的脂酶与假单胞杆菌属脂酶几乎没有同源性，并且实际上具有完全不同的特性，因此，不能用申请WO92/05249来确定哪个位置应被突变以得到具有所需特性的假单胞菌属脂酶。
本发明现提供了一种新的修饰过的脂酶，其中，至少相当于野生型、类产碱假单胞菌脂酶中21位的甲硫氨酸被另一种氨基酸取代，以得到所需的性质改变。在本发明优选的实施方案中，在使用条件下，这些脂酶变异体表现出改善的洗涤性能。优选的是其M21残基被亮氨酸、丝氨酸或丙氨酸取代的突变体。
图1：使用PCR的定点诱变
图2：将质粒pBRflank整合到染色体中
图3：从染色体中，重组缺失脂酶基因和质粒
图4：将突变体脂酶DNA导入染色体和质粒
图5：在3′侧区域重组。
在本说明书中，用术语“改善的洗涤性能”说明在应用条件下，即存在完整的洗涤剂组合物及已确立的条件下，洗涤结果改善。
在进行氨基酸序列排列比较时，铜绿色假单胞菌脂酶(Wohlfarth et al，(1992)，J.of General Microbiology 138(7)：1325-1335)16位的甲硫氨酸残基相当于类产碱假单胞菌酶在21位的甲硫氨酸。
优选地，野生型脂酶是由I类假单胞菌微生物生产的脂酶，所述野生型脂酶与类产碱假单胞菌脂酶优选地有70％或更高的同源性，同源性指：不同氨基酸序列(或蛋白质)排列比较时，相同的氨基酸数目。
我们第一次成功地得到突变体或修饰的酶，它们在工业生产条件下，表现出适当的甚至改善的洗涤性能。
使用能够使一个或多个氨基酸被任何其它所需氨基酸特异性取代的定点诱变，以构建并进一步选择具有改善了性能的酶。
具体地说，至少在相当于类产碱假单胞菌脂酶21位发生的特异性突变(其中原甲硫氨酸被另一种氨基酸取代)，在存在或不存在漂白剂的情况下，令人惊异地改善了洗涤性能，这说明，该位置对于洗涤性能的重要性，因此，在21位的甲硫氨酸取代，不仅会提高氧化稳定性，而且会改善洗涤性能。
以高度的氨基酸同源性为基础，类产碱假单胞菌脂酶与其它属于I类假单胞杆菌菌株的假单杆胞菌脂酶非常相近。这意味着这些脂酶的三维结构非常相似。因此，在假单胞菌菌株的属于I型的其它脂酶中的该结构位置进行修饰也有助于洗涤性能的改善。
适于取代所述甲硫氨酸的氨基酸实例包括Ala，Ser，Leu，Val，Phe，Asn和Asp。
在本发明优选的实施方案中，相当于野生型类产碱假单胞菌脂酶21位的原甲硫氨酸由亮氨酸取代，并表现出明显改善的性能，尽管在该突变体中，在疏水性或静电特性方面基本没有变化。
本发明也提供生产方法，包括在编码假单胞杆脂酶的DNA中，至少在相当于类产碱假单胞菌脂酶Met+21的位置上，进行突变，然后检测由所述突变产生的酶中，所需特性的变化。所述的特性变化包括：洗涤性能改善，特异性活性改变，pH活性改变，底物活性改变或氧化稳定性提高或所述特性的结合。
本发明的另一目的是提供一种选择方法，它能够选择洗涤性能提高了的突变体。在洗涤条件下确定脂酶性能的检测方法(SLM-试验)
为了本发明的目的，用所谓SLM-试验评估洗涤过程中的碱性脂酶突变体。SLM-试验使用的原理与由T.Hashimoto等人(Yukagaku 34(1985)，606-612)开展的方法的原理相同，但分析所需的时间大大减少。该方法包括：用纤维上固定的非乳化脂肪或油作为试验污渍，洗涤后提取小布块，分析提取物的脂肪及脂肪酸。根据所用的条件，洗涤过程中，形成的脂肪酸与残留的甘油三酯可以驻留在织物上，脂肪酸作为脂酶活性的效果。因此，在布块上剩余的产物数量看起来是洗涤过程中脂酶性能的良好尺度。
下面是如何优选地完成SLM试验的典型实例。用聚酯布块作为纤维，三油酸甘油酯或纯化的橄榄油(均是Sigma的产品，USA)作为底物。提取织物后，可接着对水解后的三油酸甘油酯进行色谱分析。
用于SLM试验目的的优选的洗涤过程如下：将80ml溶于正己烷(12.5％)的10mg橄榄油沾在聚酯布(3×3cm)上。室温下，将该布块空气干燥。将含10mLSTW(标准自来水：含2mM氯化钙和0.7mM氯化镁的蒸馏水)的洗涤液或溶于STW的洗涤剂放在带毛玻璃塞的Erlenmeyer烧瓶(50ml)中，然后于40℃保持在振荡水浴中。洗涤过程从将脂酶Ml(40ILU，见下文)和随后将污渍的布块加到Erlenmeyer烧瓶中开始，然后振荡40分钟。空白实验中，没有加入脂酶。洗涤后，用STW漂洗布块，随后于55℃干燥1小时，然后进行第二轮洗涤。在含5ml溶剂的玻璃试管中旋转提取干燥的布块，所述溶剂含有与底物及产物色谱分离所用的洗脱液相同的组成。
由HPLC测定所提取溶液中残留的甘油三酯量及形成的游离脂肪酸和1，2和1，3-二酰基甘油酯的量。设备及条件：泵：        LKB(2150型)检测：      折光监测仪(Jobin Yvon)注射系统：  Perkin-Elmer Iss-101；10ul积分仪：    Spectra Physics，Chromjet柱：        CP Microspher-Si(Chrompack)，100×4.6mm洗脱液：    正己烷/异丙醇/甲酸：
        975∶25∶2.5(v/v)，1ml/分钟温度：      室温
在这些条件下，三油酸甘油酯、油酸，1.3和1.2-二酰基甘油酯的保留时间分别为1.2，1.6，2.4和3.4分钟。测量峰面积或峰高。在从布块提取后，它们是三甘油酯、游离脂肪酸和二酰基甘油酯的回收尺度。以从未洗涤的布块提取后，三甘油酯的回收率为100％。在上述条件下，以峰高度为基础，发现橄榄油、油酸、1.2和1.3-二酰甘油酯之间的折光指数反应比例为1.00，0.98，2.10和1.30.确定脂酶活性的检测方法
以橄榄油的水解为基础，确定按ILU表达的本发明脂酶及突变体的活性。在30℃，存在20mM氯化钠和10Mm氯化钙的情况下，在一含10％橄榄油的0.4mM Tris缓冲液(pH9)的恒pH槽中测量水解。将1 ILU定义为在检测条件下，每分钟释放1μmol脂肪酸所需的酶量。
提供下列实施例说明但不限制本发明。
                    实施例1
研制脂酶基因的诱变系统
可以将目前的定点诱变法分成两类：
(1)缺口双链DNA由突变引物引导的错误植入(ill-in)会产生一个异源双链DNA，它含一条突变链。
(2)通过取代靶基因内的盒，可以导入适当突变的序列。然后将含所需突变的PCR片数换到表达盒内。
使用PCR的定点诱变
该方法来自Xiong，他在第三届假单胞杆菌会议(1991)上，对该方法作了综合描述。图1对该方法作了图示说明。
为了分离单链DNA，我们使用质粒pTMPv18A，在EP0334462中有描述。Vent-聚合酶(Biolabs)用于扩增反应。该聚合酶含3′校对(Proofreading)活性，因此减少了因PCR反应引起的错误掺入。
在下列缓冲液中完成PCR反应；所述缓冲液含：10mM KCl，10mM(NH4)2SO4，20mM Tris-HCl，pH8.8，2mM MgSO4，及0.1％Triton X-100，0.2mM dATP，0.2mM dCTP，0.2mM dGTP，0.2mMdTTP和1单位Vent聚合酶。
在步骤1中，将0.5μM引物A(含突变)，0.05μM引物B和1-10ng单链pTMPv18A加至50μl反应混合物中，并完成98℃ 2分钟，55℃ 2分钟；72℃ 2分钟的25个循环。
加入Klenow DNA聚合酶和连接酶，用含突变的片段作为引物补平单链DNA。这样产生一异源双链DNA，含一条突变的链。然后，用核酸外切酶处理混合物以除去单链DNA和引物A及B。然后沉淀DNA，并将其溶于PCR反应缓冲液。用含突变的新合成的DNA链作为步骤2中的模板。
步骤2中，将0.05μM引物C加到该混合物中。按上述完成第2次PCR。
然后用适当的限制酶消化PCR片数，然后将其亚克隆到整合的载体中。
                     实施例2
在活性位点附近导入突变：改变M21位
按上述导人突变。选XhoI和BclI作为适当的限制酶。所用的寡核苷酸列于表2：
                             表2
 Oligo 5′→3′ 引物号码     实变     位置
 CTGCGGGCTGGTGCTGGAGCTGCC 引物C      -   829-852R
 GGATGCAAGGATGGATCAGTGCCC 引物B      -   266-280
 CGAAGCCGAGNNNGCCGTGGGTC 引物A     M21X   426-450R
实施例3
类产碱假单胞菌M1(CBS473.85)脂酶基因的染色体失活
用自杀性整合质粒(它不能在类产碱假单胞菌中复制，但能在其它微生物中复制)使类产碱假单胞菌染色体中的脂酶基因失活。
将酯酶基因片段从质粒pTMPv18亚克隆到质粒pBR322(Bolivar等Gene 2(1977)95-113)上，该质粒能在大肠杆菌中复制，但不能在类产碱假单胞菌中复制。然后从该质粒中缺失一个内片段。所得质粒称为pBRflank。
用pBRflank转化类产碱假单胞菌M1(CBS473.85)。由于该质粒不能在假单胞菌中复制，所以只有经整合才能得到四环素抗性菌落。挑选数个四环素抗性(5mg/l)菌落。在这些菌株中，通过在5′或3′侧区域的单一重组，质粒pBRflank被整合到细菌染色体上(图2)。由于这些菌株仍含有能发挥功能的脂酶基因，因此，它们表现出脂酶阳性及四环素抗性表型。为了从染色体中缺失脂酶基因和该质粒，必须进行第二次重组(切割)。通过在没有抗生素的情况下，使菌株在BHI(脑心浸出物)培养基中生长数天，达到上述目的。
然后将细胞铺在含三丁酸甘油酯的琼脂培养基上。同样检测含脂酶阴性表型的菌落，看其是否不能在选择性琼脂盘中生长。将所得的脂酶阴性菌株称为Ps600。
在图2和3中，列出了该整合过程，然后是第二次重组的示意图。将突变的脂酶基因导入脂酶阴性菌落类产碱假单胞菌Ps600。
为了生产高产量的突变体酶，在质粒上的突变基因被整合到染色体中同时被导入相同的菌株质粒上。用与上述实施例中相似的方法，将突变脂酶基因整合到染色体中。
图4和5给出了该过程的示意图。生产突变体脂酶
按EP0334462所述，使菌株生长。然后从培养液中纯化脂酶蛋白质。EP0334462对该方法也作了描述。
                     实施例4
测定M21L突变体脂酶的特异性活性
用上述的活性检测法及对确定蛋白质含量的定量氨基酸分析，确定野生型和突变体的特异性活性。突变体的特异性活性为野生型酶的1.4倍：8000比6390ILU/mg蛋白质。
                    实施例5
在应用条件下(SLM试验)野生型脂酶M1(CBS47 3.85)和某些M21突变体的脂酶活性
按上文所述完成SLM试验。在下列条件下，于单和双循环洗涤试验中，检测野生型和突变体：——标准自来水(STW)——洗涤剂是Ariel UltraTM(2g/l)——脂酶量如所示Ariel UltraTM是Procter & Gamble的产品，可从市场上买到。该洗涤剂既不含蛋白酶也不含脂酶。
这些条件基本代表欧洲人的洗涤条件。
                      表3
有和没有脂酶M1及某些M21突变体的情况下，1和2个洗涤循环后，残留甘油三酯的百分比
                          表3
脂酶 浓度(μg/ml)     残留的甘油三酯(％)
    1个循环     2个循环
    M1M1M1M1M1M21LM21LM21LM21LM21LM21LM21AM21AM21AM21AM21AM21AM21SM21SM21SM21SM21SM21S     00.230.450.680.9100.160.310.470.631.250.250.500.751.001.502.000.250.500.751.001.502.00     8271686060845843393432605642352423696864615753     5845372920ndndndnd22281611720452933231713
nd＝未测定
从该表可以看出，所用的脂酶对织物有脂解特性。这些结果清楚地说明：当加入相同的重量时，在这些应用条件下，M21L和M21A突变体比野生型酶更有活性。M21S突变体的脂解特性可与野生型脂酶相比。
                     实施例6
根据SLM试验，在洗涤过程中，野生型脂酶(M1)与突变体M21L的性能。
在下列条件下，检测这些酶与粉末洗涤剂及漂白系统的相容性：——标准硬度的水(0.75mM的钙和0.25mM的镁)——洗涤剂是TideTM(1g/l)——按标明的脂酶量——2个循环的洗涤试验
TideTM是Procter & Gamble的产品，可从市场上买到。该洗涤剂含蛋白酶但没有脂酶。
这些条件基本代表美国的洗涤条件。
从表4可清楚地看出：实施例中所用的脂酶对织物具有脂解特性，特别是在粉末洗涤剂中的洗涤性能。在含有和不含漂白剂的洗涤剂中，该突变体酶的洗涤性能优于野生型脂酶。
                                   表4
                       脂酶M1和突变体M21L的洗涤性能
脂酶     浓度.(μg/ml) 漂白剂*                   回收(％)
  TG**     DG     FFA     总量
    M1M1M1M1M1M1M1M1M1M1M21LM21LM21LM21LM21LM21LM21LM21LM21LM21LM21LM21LM21LM21LM21LM21L     00.230.450.680.9100.230.450.680.9100.080.160.310.470.630.941.2500.080.160.310.470.630.941.25     -----+++++--------++++++++     100896362509095989497102928143353329249878503933292314     038910000010458887605898786     029121600001015172120202305152022212323     100948083769095989499102979168646156539888736863575443
*漂白系统(所用的浓度为0.3g/l)是过硼酸盐/NOBS(6.6∶1)；NOBS代表壬酰氧基苯磺酸酯**TG＝甘油三酯，DG＝甘油二酯，FFA＝游离脂肪酸
                      实施例7
根据SLM试验，在洗涤过程中野生型脂酶M1(CBS473.85)和M21S及M21A突变体的性能
在下列条件下，检测这些酶与粉末洗涤剂和漂白系统的洗涤性能和相容性：——标准硬度的水(0.75mM的钙和0.25mM的镁)——洗涤剂是TideTM(1g/l)——按标明的脂酶量——2个循环的洗涤试验
TideTM是Procter & Gamble的产品，可从市场上买到。该洗涤剂含蛋白酶但没有脂酶。在本洗涤试验中所用的布块来自在洗涤中有不同行为的不同批次。
这些条件基本代表美国的洗涤条件。
从表5可清楚地表明：本实施例中所用的脂酶在含有和不含漂白系统的洗涤剂中，对织物表现出脂解特性和其洗涤性能。
而M21S的洗涤性能可与野生型的相比，M21A的洗涤性能得到改善。
                                   表5
                 脂酶M1、突变体M21S和突变体M21A的洗涤性能
脂酶     浓度.(μg/ml) 漂白系统*                   回收(％)**
    TG     DG     FFA     总量
    M1M1M1M1M1M1M1M21SM21SM21SM21SM21SM21SM21SM21SM21SM21SM21AM21AM21AM21AM21AM21AM21AM21AM21AM21A     00.230.450.6800.230.6800.250.500.751.0000.250.500.751.0000.130.250.500.7500.130.250.500.75     ----+++-----+++++-----+++++     352416163420153525171713342317141335231516153416141110     000000000000000000000100000     0556021033540244406681104568     352921223422163528202217342521181735292124273420191718
*漂白系统(所用的浓度为0.3g/l)是过硼酸盐/NOBS(6.6∶1)；NOBS代表壬酰氧基苯磺酸酯**TG＝甘油三酯，DG＝甘油二酯，FFA＝游离脂肪酸
                     实施例8
在不同温度的应用条件下，野生型M1脂酶和M21L突变体的脂酶活性
按上文所述完成SLM试验。在下列条件下的单一洗涤试验中检测野生型和突变体：——标准自来水(STW)——洗涤剂是Ariel UltraTM(2g/l)——按标明的脂酶剂量
Ariel UltraTM是Procter & Gamble的产品，可从市场上买到。它不含酶。
                              表6
    在有和无M21L突变体和野生型脂酶M1存在下，在不同温度下一
             个洗涤循环后，残留的甘油三脂的百分比
脂酶     浓度(μg/ml)   残留的TG*(％)   在T(℃)一个洗涤循环后
    40     50     60
    -M1M1M1M1M21LM21LM21LM21L     00.210.430.640.850.200.410.610.81     756055554647423636     856664565348352525     717168787265595653
*TG＝甘油三酯
本实施例清楚地说明：在检测的应用条件下，在所有的检测温度下，突变体的脂解能力均优于野生型。在这些应用条件下，发现脂酶M1的最佳温度(甘油三酯水解的最大值)40℃，突变体的值更高：50℃。
                    实施例9
配制的脂酶M1和某些M21突变体的存放稳定性
在-PEG成粒器(PEG-Prill)中配制脂酶M1和M21L，M21S及M21A突变体。然后在下列表7中标明的温度下，将这些颗粒存于密封的玻璃试管中。贮存8个星期后，在恒pH槽中，测定脂酶的残留活性。
正如从表中看到的，在37℃贮存8个星期后，所含的所有脂酶的残留活性均为其原活性的90％以上。
                     表7
     脂酶M1和各种M21突变体的贮存稳定性
      在℃贮存的残留活性4     25     27脂酶
  酯酶M1        98    95     95M21L突变体    97    102    102M21S突变体    97    94     92M21A变体      93    95     95
根据本发明的新脂酶变异体在洗涤方面，如洗衣，洗碗盘是特别有用的，但也可将其用于本领域已知的其它方面。优选的是以颗粒(如散颗粒)或液体的形式。通常与稳定剂一起用脂酶作为洗涤剂添加剂。Met+21的取代对洗涤剂的稳定性有积极效果，其程度取决于所用的特定氨基酸。在含和不含漂白系统的情况下，Met+21突变体在溶液中的稳定性特别好。可以与其它酶，如淀粉酶、蛋白酶、纤维素酶和各种细胞壁降解酶一起使用。
本发明说明书中提到的所有公开文本(包括专利和专利申请)是与本发明有关的技术领域的专业人员可以理解的。所有公开文本均并入本文作为参考。
尽管为了清楚理解的目的，以说明及实施例的方式详细描述了本发明，但对本领域专业人员显而易见的是，在不背离本发明权利要求精神和范围内，也可以进行多种变化和修改。
                 序列目标
(1)一般信息
   (i)申请人
      (A)名称：GIST-BROCADES N.V.
      (B)街道：P.O.Box 1
      (C)城市：Delft
      (E)国家：Netherlands
      (F)邮政编码(ZIP)：NL-2600 MA
      (G)电话：31.15.791111
      (H)电话：31.15.793957
   (ii)发明题目：用于洗涤剂领域的新的脂酶变异体
   (iii)序列数目：3
   (iv)计算机阅读形式
       (A)介质类型Floppg盘
       (B)计算机：兼容
       (C)操作系统：PC-DOS/MS-DOS
       (D)软件 PatentIn Release #1.0，Version #1.25(EPO)
   (vi)在先中请资料：
       (A)申请号： EP 93201212.3
       (B)申请日：27-APR-1993
(2)SEQ ID No.1的资料：
   (i)序列特征：
      (A)长度：23个碱基对
      (B)类型：核酸
      (C)链型：单链
      (D)拓扑结构：线性
   (ii)分子类型 ：DNA(合成)
   (iii)假设：无
   (vi)来源：
       (C)个体分离物：引物A
   (xi)序列描述 ：SEQ ID NO：1：CGAAGCCGAG NNNGCCGTGG GTC
(2)SEQ ID No.2的资料
   (i)序列特征：
       (A)长度：24个碱基对
       (B)类型：核酸
       (C)链型：单链
       (D)拓扑结构：线性
   (ii)分子类型：DNA(合成的)
   (iii)假设：无
   (vi)来源：
       (C)个体分离物 ：引物B
   (xi)序列描述：SEQ ID No：2：GGATGCAAGG ATGGATCAGT GCCC
(2)SEQ ID No.3的资料
   (i)序列特征：
      (A)长度：24个碱基对
      (B)类型：核酸
      (C)链型：单链
      (D)拓扑结构：线性
   (ii)分子类型：DNA(合成的)
   (iii)假设：无
   (vi)来源：
      (C)个体分离物：引物C
   (xi)序列描述 ：SEQ ID No：3：CTGCGGGCTG GTGCTGGAGC TGCC

Claims (13)

1、一种修饰过的脂酶，其中至少相当于野生型类产碱假单胞菌(Pseudomonas Pseudoalcaligenes)脂酶21位的甲硫氨酸被另一种氨基酸取代，所述脂酶表现出所需的特性变化。
2、根据权利要求1的修饰的脂酶，其中野生型脂酶来源于I型假单胞菌(Pseudomonas)微生物。
3、根据权利要求1或2的修饰的脂酶，其中野生型脂酶与类产碱假单胞菌脂酶有至少70％的同源性。
4、根据权利要求1-3之任一的修饰的脂酶，其中21位上的甲硫氨酸被Leu，Ser或Ala取代。
5、根据权利要求1-4之任一的修饰的脂酶，表现出改善的洗涤性能。
6、一种洗涤剂组合物，含有权利要求1-5之任一的修饰的脂酶。
7、权利要求1-5之任一的修饰的脂酶作为洗涤剂组分的用途。
8、一种重组DNA序列，其编码权利要求1-5之任一的修饰的脂酶。
9、一种重组DNA载体，其含权利要求8的重组DNA序列并可用于表达修饰的脂酶。
10、一种转化的宿主微生物，其含有权利要求8的重组DNA序列或权利要求9的重组DNA载体。
11、根据权利要求10的微生物，转化的宿主微生物是假单胞菌。
12、一种方法，包括在至少相当于类产碱假单胞菌脂酶Met+21的位置，在编码假单胞杆菌脂酶的DNA中进行突变，并检测在由所述突变产生的酶中，所需特性的变化。
13、根据权利要求12的方法，其中所述特性变化选自洗涤性能改善、特异性活性改变、pH活性改变、底物活性改变和氧化稳定性提高的一种或多种。
CN94190243A 1993-04-27 1994-04-27 用于洗涤剂领域的新的脂酶变异体 Pending CN1108457A (zh)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP93201212 1993-04-27
EP93201212.3 1993-04-27

Publications (1)

Publication Number Publication Date
CN1108457A true CN1108457A (zh) 1995-09-13

Family

ID=8213797

Family Applications (1)

Application Number Title Priority Date Filing Date
CN94190243A Pending CN1108457A (zh) 1993-04-27 1994-04-27 用于洗涤剂领域的新的脂酶变异体

Country Status (12)

Country Link
EP (1) EP0652946B1 (zh)
JP (1) JP3618748B2 (zh)
KR (1) KR950702240A (zh)
CN (1) CN1108457A (zh)
AT (1) ATE287946T1 (zh)
AU (1) AU673078B2 (zh)
CA (1) CA2138519C (zh)
DE (1) DE69434242T2 (zh)
DK (1) DK0652946T3 (zh)
FI (1) FI116728B (zh)
PL (1) PL306812A1 (zh)
WO (1) WO1994025578A1 (zh)

Families Citing this family (577)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5772786A (en) * 1993-08-13 1998-06-30 The Procter & Gamble Company Detergent composition comprising lime soap dispersant and lipase enzymes
EP0746618B1 (en) * 1994-02-22 2002-08-21 Novozymes A/S A method of preparing a variant of a lipolytic enzyme
DE4411349A1 (de) * 1994-03-31 1995-10-05 Henkel Kgaa Lipasehaltiges Textilwaschmittel
US6495357B1 (en) * 1995-07-14 2002-12-17 Novozyme A/S Lipolytic enzymes
JPH09275977A (ja) 1996-04-18 1997-10-28 Novo Nordisk As 新規リパーゼおよび洗浄剤組成物
EP0954569A1 (en) * 1996-08-27 1999-11-10 Novo Nordisk A/S Novel lipolytic enzymes
EP0943678A3 (en) * 1998-02-18 2000-05-10 Novo Nordisk A/S Lipase variants
US6156552A (en) * 1998-02-18 2000-12-05 Novo Nordisk A/S Lipase variants
ATE504651T1 (de) 1998-12-18 2011-04-15 Novozymes As Subtilase enzyme der i-s1- und i-s2-untergruppen mit einem zusätzlichen aminosäurerest in einer aktiven schleifenregion
WO2000060060A2 (en) 1999-03-31 2000-10-12 Novozymes A/S Polypeptides having alkaline alpha-amylase activity and nucleic acids encoding same
ES2322426T3 (es) 1999-03-31 2009-06-22 Novozymes A/S Polipeptidos con actividad alfa-amilasa y acidos nucleicos que codifican a los mismos.
DE60040284D1 (de) 1999-05-20 2008-10-30 Novozymes As Subtilase enzyme der i-s1 und i-s2 untergruppen mit mindestens einer zusätzlichen aminosäure zwischen position 129 und 130
WO2000071690A1 (en) 1999-05-20 2000-11-30 Novozymes A/S Subtilase enzymes of the i-s1 and i-s2 sub-groups having at least one additional amino acid residue between positions 128 and 129
ATE408679T1 (de) 1999-05-20 2008-10-15 Novozymes As Subtilase enzyme der i-s1 und i-s2 untergruppen mit mindestens einer zusätzlichen aminosäure zwischen position 127 und 128
EP1183340B1 (en) 1999-05-20 2008-09-03 Novozymes A/S Subtilase enzymes of the i-s1 and i-s2 sub-groups having at least one additional amino acid residue between positions 126 and 127
ATE402996T1 (de) 1999-05-20 2008-08-15 Novozymes As Subtilase-enzyme der i-s1 und i-s2 untergruppen mit wenigstens einem zusätzlichen aminosäurerest zwischen positionen 125 und 126
DE60040282D1 (de) 1999-05-20 2008-10-30 Novozymes As SUBTILASE ENZYME DER I-S1 UND I-S2 UNTERGRUPPEN MIT MINDESTENS EINER ZUSäTZLICHEN AMINOSäURE ZWISCHEN POSITION 132 UND 133
EP1214426A2 (en) 1999-08-31 2002-06-19 Novozymes A/S Novel proteases and variants thereof
CA2419896C (en) 2000-08-21 2014-12-09 Novozymes A/S Subtilase enzymes
EP2333055A1 (en) 2000-10-13 2011-06-15 Novozymes A/S Subtilase variants
US20040091994A1 (en) 2000-10-13 2004-05-13 Carsten Andersen Alpha-amylase variant with altered properties
EP2159279A3 (en) 2001-05-15 2010-05-12 Novozymes A/S Alpha-amylase variant with altered properties
US20030051836A1 (en) 2001-05-21 2003-03-20 Novozymes A/S Enzymatic hydrolysis of a polymer comprising vinyl acetate monomer
ES2438146T3 (es) 2001-06-26 2014-01-16 Novozymes A/S Polipéptidos que tienen actividad de celobiohirolasa I y polinucleótidos que codifican los mismos
DK200101090A (da) 2001-07-12 2001-08-16 Novozymes As Subtilase variants
CN100386434C (zh) 2002-03-27 2008-05-07 诺和酶股份有限公司 具有丝状包衣的颗粒
DE60325731D1 (en) 2002-07-01 2009-02-26 Novozymes As Monopropylenglykol als fermentationszusatz
ATE494368T1 (de) 2002-10-01 2011-01-15 Novozymes As Polypeptide der gh-61-familie
TWI319007B (en) 2002-11-06 2010-01-01 Novozymes As Subtilase variants
DK1578964T4 (da) 2002-12-20 2013-12-02 Novozymes As Polypeptider med cellobiohydrolase II-aktivitet og polynukleotider, der koder for samme
JP2006517990A (ja) 2003-01-27 2006-08-03 ノボザイムス アクティーゼルスカブ 粒体の安定化
JP2006517989A (ja) 2003-02-18 2006-08-03 ノボザイムス アクティーゼルスカブ 洗剤組成物
US7413888B2 (en) 2003-05-02 2008-08-19 Novozymes, Inc. Variants of beta-glucosidases
ES2346654T3 (es) 2003-06-19 2010-10-19 Novozymes A/S Proteasas.
AU2004252572B2 (en) 2003-06-25 2011-09-08 Novozymes A/S Polypeptides having alpha-amylase activity and polypeptides encoding same
ES2424346T3 (es) 2003-08-25 2013-10-01 Novozymes Inc. Variantes de glucósido hidrolasas
EP2308966A1 (en) 2003-10-10 2011-04-13 Novozymes A/S Protease variants
CN1871344A (zh) 2003-10-23 2006-11-29 诺和酶股份有限公司 在洗涤剂中具有改善稳定性的蛋白酶
WO2005047499A1 (en) 2003-10-28 2005-05-26 Novozymes Inc. Polypeptides having beta-glucosidase activity and polynucleotides encoding same
EP1709165B1 (en) 2004-01-06 2014-04-23 Novozymes A/S Polypeptides of alicyclobacillus
EP2305702B1 (en) 2004-01-30 2014-03-19 Novozymes Inc. Polypeptides having cellulolytic enhancing activity and polynucleotides encoding same
WO2005078074A2 (en) 2004-02-13 2005-08-25 Novozymes A/S Protease variants
US7148404B2 (en) 2004-05-04 2006-12-12 Novozymes A/S Antimicrobial polypeptides
WO2005123911A2 (en) 2004-06-21 2005-12-29 Novozymes A/S Proteases
WO2006002643A2 (en) 2004-07-05 2006-01-12 Novozymes A/S Alpha-amylase variants with altered properties
ATE554163T1 (de) 2004-09-21 2012-05-15 Novozymes As Subtilasen
US7741095B2 (en) 2004-09-21 2010-06-22 Novozymes A/S Subtilases
US7811979B2 (en) 2004-09-21 2010-10-12 Novozymes A/S Subtilases
CN101084307B (zh) 2004-09-30 2011-06-01 诺维信股份有限公司 具有脂肪酶活性的多肽和编码其的多核苷酸
US7824884B2 (en) 2005-04-27 2010-11-02 Novozymes, Inc. Polypeptides having endoglucanase activity and polynucleotides encoding same
EP2290061A3 (en) 2005-07-08 2011-07-06 Novozymes A/S Subtilase variants
CN101243182B (zh) 2005-08-16 2014-08-06 诺维信公司 枯草蛋白酶
US8119387B2 (en) 2005-08-16 2012-02-21 Novozymes A/S Polypeptides of strain Bacillus sp. P203
US9303256B2 (en) 2005-09-30 2016-04-05 Novozymes A/S Immobilization of enzymes
EP1941023B1 (en) 2005-09-30 2017-04-05 Novozymes Inc. Methods for enhancing the degradation or conversion of cellulosic material
EP1994130A1 (en) 2006-03-02 2008-11-26 Novozymes A/S High capacity encapsulation process
US7883711B2 (en) 2006-03-22 2011-02-08 Novozymes A/S Use of polypeptides having antimicrobial activity
EP2383330A1 (en) 2006-03-31 2011-11-02 Novozymes A/S A stabilized liquid enzyme composition
PT2007942E (pt) 2006-04-14 2014-10-07 Danisco Us Inc Tratamento de têxteis num passo
CA2658610A1 (en) 2006-07-21 2008-05-15 Novozymes, Inc. Methods of increasing secretion of polypeptides having biological activity
EP2066785B1 (en) 2006-08-11 2023-02-01 Novozymes Biologicals, Inc. Bacteria cultures and compositions comprising bacteria cultures
DK2074205T4 (en) 2006-10-06 2017-02-06 Novozymes As CELLULOTIC ENZYME COMPOSITIONS AND APPLICATIONS THEREOF
JP2010512787A (ja) 2006-12-21 2010-04-30 ダニスコ・ユーエス・インク、ジェネンコー・ディビジョン バシルス（Ｂａｃｉｌｌｕｓ）種１９５のアルファ‐アミラーゼポリペプチドの組成物と使用。
CA2680158A1 (en) 2007-03-09 2008-09-18 Danisco Us Inc. Alkaliphilic bacillus species .alpha.-amylase variants, compositions comprising .alpha.-amylase variants, and methods of use
WO2008118749A2 (en) 2007-03-23 2008-10-02 Novozymes Biologicals, Inc. Preventing and reducing biofilm formation and planktonic proliferation
DE102007016139A1 (de) 2007-03-30 2008-10-02 Jenabios Gmbh Verfahren zur regioselektiven Oxygenierung von N-Heterozyklen
BRPI0819184B1 (pt) 2007-11-05 2022-05-10 Danisco Us Inc Variantes de alfa-amilase com propriedades alteradas, polinucleotídeo, vetor, célula hospedeira, composição, kit, bem como métodos para tratamento de um caldo de amido, para produção de um substrato fermentável, e para tratamento de um material contendo amido
DK2215202T3 (da) 2007-11-05 2017-11-27 Danisco Us Inc VARIANTER AF BACILLUS sp. TS-23 ALPHA-AMYLASE MED ÆNDREDE EGENSKABER
WO2009100102A2 (en) 2008-02-04 2009-08-13 Danisco Us Inc., Genencor Division Ts23 alpha-amylase variants with altered properties
CN102016044B (zh) 2008-04-30 2014-11-26 丹尼斯科美国公司 新型嵌合α-淀粉酶变体
EP2300605A2 (en) 2008-06-06 2011-03-30 Danisco US Inc. Geobacillus stearothermophilus alpha-amylase (amys) variants with improved properties
WO2009149271A2 (en) 2008-06-06 2009-12-10 Danisco Us Inc. Production of glucose from starch using alpha-amylases from bacillus subtilis
JP5599113B2 (ja) 2008-06-06 2014-10-01 ダニスコ・ユーエス・インク 糖化酵素組成物及びその糖化方法
CA2726803A1 (en) 2008-06-06 2009-12-10 Danisco Us Inc. Variant alpha-amylases from bacillus subtilis and methods of use, thereof
EP2149786A1 (en) 2008-08-01 2010-02-03 Unilever PLC Improvements relating to detergent analysis
ES1076140Y (es) 2008-09-12 2012-05-08 Unilever Nv Dispensador y pretratador para liquidos viscosos
CN102224234B (zh) 2008-09-25 2014-04-16 丹尼斯科美国公司 α-淀粉酶混合物和使用所述混合物的方法
WO2010059413A2 (en) 2008-11-20 2010-05-27 Novozymes, Inc. Polypeptides having amylolytic enhancing activity and polynucleotides encoding same
BRPI0922773B1 (pt) 2008-12-04 2018-10-09 Novozymes As célula microbiana hospedeira transgênica, métodos para produzir um polipeptídeo tendo atividade de intensificação celulolítica e para degradar ou converter um material celulósico, construção de ácido nucleico, vetor de expressão, e, composição detergente.
EP2376527A1 (en) 2008-12-12 2011-10-19 Novozymes Inc. Polypeptides having lipase activity and polynucleotides encoding same
EP2202290A1 (en) 2008-12-23 2010-06-30 Unilever PLC A flowable laundry composition and packaging therefor
EP2213723A1 (en) 2009-01-30 2010-08-04 Novozymes A/S Isomaltose for fungus fermentation
US20110306139A1 (en) 2009-02-27 2011-12-15 Novozymes A/S Mutant Cells Suitable For Recombinant Polypeptide Production
WO2010104675A1 (en) 2009-03-10 2010-09-16 Danisco Us Inc. Bacillus megaterium strain dsm90-related alpha-amylases, and methods of use, thereof
US8293697B2 (en) 2009-03-18 2012-10-23 The Procter & Gamble Company Structured fluid detergent compositions comprising dibenzylidene sorbitol acetal derivatives
US8153574B2 (en) 2009-03-18 2012-04-10 The Procter & Gamble Company Structured fluid detergent compositions comprising dibenzylidene polyol acetal derivatives and detersive enzymes
CA2757343A1 (en) 2009-04-01 2010-10-07 Danisco Us Inc. Compositions and methods comprising alpha-amylase variants with altered properties
WO2010117511A1 (en) 2009-04-08 2010-10-14 Danisco Us Inc. Halomonas strain wdg195-related alpha-amylases, and methods of use, thereof
BRPI1011411A2 (pt) 2009-05-05 2016-03-15 Unilever Nv composição de tratamento para lavagem de tecidos, e, método doméstico de tratamento de tecidos
DK3269804T3 (da) 2009-09-17 2020-11-23 Novozymes Inc Polypeptider med cellulolyseforbedrende aktivitet og polynukleotider, som koder for dem
US20120252106A1 (en) 2009-09-25 2012-10-04 Novozymes A/S Use of Protease Variants
RU2651525C2 (ru) 2009-09-25 2018-04-19 Новозимс А/С Варианты субтилаз
CA2775358A1 (en) 2009-09-29 2011-04-07 Novozymes A/S Polypeptides having cellulolytic enhancing activity and polynucleotides encoding same
DK2483296T3 (en) 2009-09-30 2015-11-02 Novozymes Inc Polypeptides having cellulolytic enhancing activity and polynucleotides encoding them
CN102666846A (zh) 2009-09-30 2012-09-12 诺维信公司 具有纤维素分解增强活性的多肽和编码该多肽的多核苷酸
US8673024B2 (en) 2009-10-08 2014-03-18 Conopco Inc. Shading composition
ES2532473T3 (es) 2009-10-13 2015-03-27 Unilever N.V. Polímeros colorantes
BR112012009143A2 (pt) 2009-10-21 2015-09-15 Novozymes As método para tratamento de óleo que contém ácidos solúveis em água, e, partículas de sílica amorfa porosa que contêm base
BR112012009659A2 (pt) 2009-10-23 2015-09-15 Danisco Us Inc métodos para redução de sacarídeo azul.
WO2011047987A1 (en) 2009-10-23 2011-04-28 Unilever Plc Dye polymers
WO2011078949A1 (en) 2009-12-21 2011-06-30 Danisco Us Inc. Surfactants that improve the cleaning of lipid-based stains treated with lipases
WO2011080267A2 (en) 2009-12-29 2011-07-07 Novozymes A/S Polypetides having detergency enhancing effect
WO2011082889A1 (en) 2010-01-07 2011-07-14 Unilever Plc Natural shading agents
AU2011208429B2 (en) 2010-01-22 2014-05-22 Dupont Nutrition Biosciences Aps Methods for producing amino-substituted glycolipid compounds
ES2477518T3 (es) 2010-02-09 2014-07-17 Unilever Nv Polímeros colorantes
EP2534237B1 (en) 2010-02-12 2014-11-12 Unilever PLC Laundry treatment composition comprising bis-azo shading dyes
US8859259B2 (en) 2010-02-14 2014-10-14 Ls9, Inc. Surfactant and cleaning compositions comprising microbially produced branched fatty alcohols
CN102782126A (zh) 2010-02-18 2012-11-14 丹尼斯科美国公司 得自涅斯捷连科氏菌(Nesterenkonia)的淀粉酶及其使用方法
JP5833576B2 (ja) 2010-02-25 2015-12-16 ノボザイムス アクティーゼルスカブ リゾチームの変異体及びそれをコードするポリヌクレオチド
CN102892875A (zh) 2010-04-29 2013-01-23 荷兰联合利华有限公司 双杂环偶氮染料
ES2609023T3 (es) 2010-07-22 2017-04-18 Unilever N.V. Composiciones detergentes que comprenden biotensioactivo y enzima
CN103052704A (zh) 2010-07-22 2013-04-17 荷兰联合利华有限公司 用于提高清洁的鼠李糖脂和酶的组合物
BR112013000114B1 (pt) 2010-07-22 2020-12-29 Unilever N.V. composição de limpeza e processo para limpeza de um substrato
WO2012028482A1 (en) 2010-08-30 2012-03-08 Novozymes A/S A two-soak wash
EP2611898A1 (en) 2010-08-30 2013-07-10 Novozymes A/S A concentrated soak wash
WO2012035103A1 (en) 2010-09-16 2012-03-22 Novozymes A/S Lysozymes
GB201015672D0 (en) 2010-09-20 2010-10-27 Unilever Plc Improvements relating to fabric treatment compositions comprising targeted benefit agents
WO2012044836A1 (en) 2010-09-30 2012-04-05 Novozymes, Inc. Variants of polypeptides having cellulolytic enhancing activity and polynucleotides encoding same
DK2622068T3 (en) 2010-09-30 2016-10-17 Novozymes Inc Variants of polypeptides having cellulolytic enhancing ACTIVITY AND POLYNUCLEOTIDES ENCODING THEM
WO2012049034A1 (en) 2010-10-14 2012-04-19 Unilever Plc Packaging and dispensing of detergent compositions
ES2617553T3 (es) 2010-10-14 2017-06-19 Unilever N.V. Partículas de detergente para lavado de ropa
ES2602176T3 (es) 2010-10-14 2017-02-17 Unilever N.V. Partículas de detergente para lavado de ropa
EP2627753B1 (en) 2010-10-14 2016-11-02 Unilever PLC Laundry detergent particle
CA2813793C (en) 2010-10-14 2019-05-07 Unilever Plc Laundry detergent particles
CN103282477B (zh) 2010-10-14 2015-04-01 荷兰联合利华有限公司 顶部装载洗衣容器的方法
WO2012049032A1 (en) 2010-10-14 2012-04-19 Unilever Plc Refill and refillable packages of concentrated particulate detergent compositions
AU2011316077B2 (en) 2010-10-14 2014-01-23 Unilever Plc Packaged particulate detergent composition
EP2441820A1 (en) 2010-10-14 2012-04-18 Unilever Plc, A Company Registered In England And Wales under company no. 41424 of Unilever House Laundry detergent particles
US9062281B2 (en) 2010-10-14 2015-06-23 Conopco, Inc. Particulate detergent compositions comprising fluorescer
ES2613702T3 (es) 2010-10-14 2017-05-25 Unilever N.V. Partículas de detergente para lavado de ropa
BR112013009129B1 (pt) 2010-10-14 2020-10-13 Unilever N.V. partícula de detergente revestida
US8883702B2 (en) 2010-10-14 2014-11-11 Conopco, Inc. Packaged particulate detergent composition
CN103153813B (zh) 2010-10-14 2015-08-19 荷兰联合利华有限公司 包含洗衣组合物的包装、所述包装的分配器和利用所述分配器和所述包装进行洗涤的方法
EP2441822A1 (en) 2010-10-14 2012-04-18 Unilever Plc, A Company Registered In England And Wales under company no. 41424 of Unilever House Laundry detergent particles
EP2441825A1 (en) 2010-10-14 2012-04-18 Unilever Plc, A Company Registered In England And Wales under company no. 41424 of Unilever House Process for preparing laundry detergent particles
CN103180222B (zh) 2010-10-14 2016-01-20 荷兰联合利华有限公司 包装的浓缩颗粒洗涤剂组合物
ES2644555T3 (es) 2010-10-14 2017-11-29 Unilever N.V. Embalaje y distribución de composiciones detergentes
CN103153812B (zh) 2010-10-14 2016-04-06 荷兰联合利华有限公司 洗涤剂组合物的透明包装
WO2012052306A1 (en) 2010-10-22 2012-04-26 Unilever Plc Externally structured aqueous detergent liquid
EP2635666B1 (en) 2010-11-01 2014-07-23 Unilever N.V. A detergent composition having shading dyes and lipase
DK2640833T3 (en) 2010-11-18 2016-11-28 Novozymes Inc Chimeric polypeptides having cellulolytic enhancing ACTIVITY AND POLYNUCLEOTIDES ENCODING THEM
WO2012098046A1 (en) 2011-01-17 2012-07-26 Unilever Plc Dye polymer for laundry treatment
MY163351A (en) 2011-01-31 2017-09-15 Unilever Plc Alkaline liquid detergent compositions
ES2656049T3 (es) 2011-01-31 2018-02-22 Novozymes A/S Uso de glucosa tostada para la producción fermentativa de taxtomina
PL2675891T3 (pl) 2011-02-15 2019-03-29 Novozymes Biologicals, Inc. Łagodzenie zapachu w maszynach czyszczących i procesach czyszczenia
MX2013009178A (es) 2011-02-16 2013-08-29 Novozymes As Composiciones detergentes que comprenden metaloproteasas.
JP2014511409A (ja) 2011-02-16 2014-05-15 ノボザイムス アクティーゼルスカブ 金属プロテアーゼを含む洗剤組成物
WO2012110564A1 (en) 2011-02-16 2012-08-23 Novozymes A/S Detergent compositions comprising m7 or m35 metalloproteases
MX2013007997A (es) 2011-02-23 2013-08-21 Novozymes Inc Polipeptidos que tienen actividad de incremento celulolitico y polinucleotidos que codifican para los mismos.
VN36510A1 (en) 2011-03-10 2014-01-27 Unilever Plc No 41424 Dye polymer
WO2012130492A1 (en) 2011-03-25 2012-10-04 Unilever Plc Dye polymer
US9410136B2 (en) 2011-03-31 2016-08-09 Novozymes, Inc. Methods for enhancing the degradation or conversion of cellulosic material
EP2476743B1 (en) 2011-04-04 2013-04-24 Unilever Plc, A Company Registered In England And Wales under company no. 41424 of Unilever House Method of laundering fabric
WO2012149344A1 (en) 2011-04-29 2012-11-01 Novozymes, Inc. Methods for enhancing the degradation or conversion of cellulosic material
EP2522715A1 (en) 2011-05-13 2012-11-14 Unilever Plc, A Company Registered In England And Wales under company no. 41424 of Unilever House Aqueous concentrated laundry detergent compositions
EP2522714A1 (en) 2011-05-13 2012-11-14 Unilever Plc, A Company Registered In England And Wales under company no. 41424 of Unilever House Aqueous concentrated laundry detergent compositions
WO2012159778A1 (en) 2011-05-26 2012-11-29 Unilever Plc Liquid laundry composition
WO2012163871A1 (en) 2011-06-01 2012-12-06 Unilever Plc Liquid detergent composition containing dye polymer
CN103620029B (zh) 2011-06-24 2017-06-09 诺维信公司 具有蛋白酶活性的多肽和编码它们的多核苷酸
CN109097347A (zh) 2011-06-30 2018-12-28 诺维信公司 α-淀粉酶变体
MX351850B (es) 2011-06-30 2017-10-31 Novozymes As Metodo para el cribado de alfa-amilasas.
WO2013004635A1 (en) 2011-07-01 2013-01-10 Novozymes A/S Liquid detergent composition
CN103649292B (zh) 2011-07-01 2017-11-28 诺维信公司 稳定化的枯草杆菌蛋白酶组合物
EP2732018B1 (en) 2011-07-12 2017-01-04 Novozymes A/S Storage-stable enzyme granules
EP2734610B1 (en) 2011-07-21 2015-09-09 Unilever PLC Liquid laundry composition
WO2013024021A1 (en) 2011-08-15 2013-02-21 Novozymes A/S Polypeptides having cellulase activity and polynucleotides encoding same
CN103764822B (zh) 2011-08-19 2016-11-23 诺维信公司 具有蛋白酶活性的多肽
EP2756063B1 (en) 2011-09-15 2017-10-04 Unilever PLC Detergent compositions comprising surfactant and enzyme
US20140227738A1 (en) 2011-09-22 2014-08-14 Novozymes A/S Polypeptides Having Protease Activity and Polynucleotides Encoding Same
AU2012328562A1 (en) 2011-10-28 2014-03-13 Danisco Us Inc. Variant maltohexaose-forming alpha-amylase variants
EP3597736A1 (en) 2011-11-21 2020-01-22 Novozymes A/S Gh61 polypeptide variants and polynucleotides encoding same
CN103957929B (zh) 2011-11-25 2017-06-30 诺维信公司 具有溶菌酶活性的多肽和编码所述多肽的多核苷酸
WO2013076269A1 (en) 2011-11-25 2013-05-30 Novozymes A/S Subtilase variants and polynucleotides encoding same
WO2013087027A1 (en) 2011-12-16 2013-06-20 Novozymes, Inc. Polypeptides having laccase activity and polynucleotides encoding same
EP2607468A1 (en) 2011-12-20 2013-06-26 Henkel AG & Co. KGaA Detergent compositions comprising subtilase variants
EP2794832B1 (en) 2011-12-20 2016-05-25 Unilever Plc. Isotropic liquid detergents comprising soil release polymer
CN103998593B (zh) 2011-12-20 2017-05-03 荷兰联合利华有限公司 包含脂肪酶和漂白催化剂的液体洗涤剂
CN107267489A (zh) 2011-12-20 2017-10-20 诺维信公司 枯草杆菌酶变体和编码它们的多核苷酸
IN2014DN03298A (zh) 2011-12-22 2015-06-26 Danisco Us Inc
EP2794866A1 (en) 2011-12-22 2014-10-29 Danisco US Inc. Compositions and methods comprising a lipolytic enzyme variant
US10174301B2 (en) 2011-12-28 2019-01-08 Novozymes A/S Methods for improving the nutritional value of the animal feed using a protease
CN104024393A (zh) 2011-12-29 2014-09-03 诺维信公司 具有脂肪酶变体的洗涤剂组合物
CN104350149A (zh) 2012-01-26 2015-02-11 诺维信公司 具有蛋白酶活性的多肽在动物饲料和洗涤剂中的用途
EP2628785B1 (en) 2012-02-17 2016-05-18 Henkel AG & Co. KGaA Detergent compositions comprising subtilase variants
MX350713B (es) 2012-02-17 2017-09-14 Novozymes As Variantes de subtilisina y polinucleotidos que las codifican.
WO2013131964A1 (en) 2012-03-07 2013-09-12 Novozymes A/S Detergent composition and substitution of optical brighteners in detergent compositions
EP2639291A1 (en) 2012-03-13 2013-09-18 Unilever PLC Packaged particulate detergent composition
US10087401B2 (en) 2012-03-16 2018-10-02 Monosol, Llc Water soluble compositions incorporating enzymes, and method of making same
WO2013139702A1 (en) 2012-03-21 2013-09-26 Unilever Plc Laundry detergent particles
EP2831215B1 (en) 2012-03-29 2018-08-08 Novozymes A/S Use of enzymes for preparing water soluble films
CN104204198B (zh) 2012-04-02 2018-09-25 诺维信公司 脂肪酶变体以及编码其的多核苷酸
US20150038393A1 (en) 2012-04-03 2015-02-05 Conopco, Inc., D/B/A Unilever Laundry detergent particles
CN104220582B (zh) 2012-04-03 2017-12-22 荷兰联合利华有限公司 洗衣洗涤剂颗粒
MX2014011547A (es) 2012-04-03 2014-11-14 Unilever Nv Particulas de detergente para lavado de ropa.
WO2013149755A1 (en) 2012-04-03 2013-10-10 Unilever Plc Laundry detergent particles
US9394092B2 (en) 2012-04-16 2016-07-19 Monosol, Llc Powdered pouch and method of making same
CN104379716A (zh) 2012-04-23 2015-02-25 荷兰联合利华有限公司 结构化的水性液体洗涤剂
EP2841567B1 (en) 2012-04-27 2017-07-26 Novozymes, Inc. Gh61 polypeptide variants and polynucleotides encoding same
ES2643216T3 (es) 2012-05-07 2017-11-21 Novozymes A/S Polipéptidos con actividad de degradación de xantano y polinucleótidos que codifican la misma
CA2870830A1 (en) 2012-05-11 2013-11-14 Danisco Us Inc. Use of alpha-amylase from aspergillus clavatus for saccharification
US20150132831A1 (en) 2012-05-16 2015-05-14 Novozymes A/S Compositions Comprising Lipase and Methods of Use Thereof
DK2825643T3 (da) 2012-06-08 2021-11-08 Danisco Us Inc Variant-alfa-amylaser med forbedret aktivitet over for stivelsespolymerer
EP2861749A1 (en) 2012-06-19 2015-04-22 Novozymes Bioag A/S Enzymatic reduction of hydroperoxides
BR112014031882A2 (pt) 2012-06-20 2017-08-01 Novozymes As uso de um polipeptídeo isolado, polipeptídeo, composição, polinucleotídeo isolado, construto de ácido nucleico ou vetor de expressão, célula hospedeira de expressão recombinante, métodos para produção de um polipeptídeo, para melhoria do valor nutricional de uma ração animal, e para o tratamento de proteínas, uso de pelo menos um polipeptídeo, aditivo de ração animal, ração animal, e, composição detergente
US9663804B2 (en) 2012-07-18 2017-05-30 Novozymes A/S Method of treating polyester textile
WO2014026630A1 (en) 2012-08-16 2014-02-20 Novozymes A/S Method for treating textile with endoglucanase
US20150218606A1 (en) 2012-08-16 2015-08-06 Danisco Us Inc. Method of using alpha-amylase from aspergillus clavatus and pullulanase for saccharification
BR112015003726A2 (pt) 2012-08-22 2019-09-24 Novozymes As composição detergente, uso de uma composição e de um polipeptídeo, e, método para remoção de uma nódoa de uma superfície.
MX2015002211A (es) 2012-08-22 2015-05-08 Novozymes As Metaloproteasa de exiguobacterium.
CN104603266B (zh) 2012-08-22 2017-11-10 诺维信公司 来自脂环酸芽孢杆菌属的金属蛋白酶
US9688948B2 (en) 2012-09-25 2017-06-27 Conopco, Inc. Laundry detergent particles
US20150291656A1 (en) 2012-11-01 2015-10-15 Novozymes A/S Method for removal of dna
WO2014081622A1 (en) 2012-11-20 2014-05-30 Danisco Us Inc. Amylase with maltogenic properties
EP2929004B1 (en) 2012-12-07 2019-05-15 Novozymes A/S Preventing adhesion of bacteria
JP6499081B2 (ja) 2012-12-11 2019-04-10 ダニスコ・ユーエス・インク アスペルギルス・フミガタス（Ａｓｐｅｒｇｉｌｌｕｓｆｕｍｉｇａｔｕｓ）由来のグルコアミラーゼを発現するトリコデルマ・レーシ（Ｔｒｉｃｈｏｄｅｒｍａｒｅｅｓｅｉ）宿主細胞、及びその使用方法
CA2894513A1 (en) 2012-12-14 2014-06-19 Danisco Us Inc. Method of using alpha-amylase from aspergillus fumigatus and isoamylase for saccharification
WO2014090940A1 (en) 2012-12-14 2014-06-19 Novozymes A/S Removal of skin-derived body soils
EP2904105A1 (en) 2012-12-20 2015-08-12 Danisco US Inc. Method of using alpha-amylase from aspergillus terreus and pullulanase for saccharification
US20160053243A1 (en) 2012-12-21 2016-02-25 Danisco Us Inc. Alpha-amylase variants
WO2014099525A1 (en) 2012-12-21 2014-06-26 Danisco Us Inc. Paenibacillus curdlanolyticus amylase, and methods of use, thereof
CN104869841A (zh) 2012-12-21 2015-08-26 诺维信公司 具有蛋白酶活性的多肽和编码它的多核苷酸
WO2014106593A1 (en) 2013-01-03 2014-07-10 Novozymes A/S Alpha-amylase variants and polynucleotides encoding same
HUE039341T2 (hu) 2013-03-11 2018-12-28 Danisco Us Inc Alfa-amiláz kombinatorikus variációi
US20160024440A1 (en) 2013-03-14 2016-01-28 Novozymes A/S Enzyme and Inhibitor Containing Water-Soluble Films
WO2014147127A1 (en) 2013-03-21 2014-09-25 Novozymes A/S Polypeptides with lipase activity and polynucleotides encoding same
EP3569611A1 (en) 2013-04-23 2019-11-20 Novozymes A/S Liquid automatic dish washing detergent compositions with stabilised subtilisin
EP2992076B1 (en) 2013-05-03 2018-10-24 Novozymes A/S Microencapsulation of detergent enzymes
AR096270A1 (es) 2013-05-14 2015-12-16 Novozymes As Composiciones detergentes
US20160083703A1 (en) 2013-05-17 2016-03-24 Novozymes A/S Polypeptides having alpha amylase activity
EP3786269A1 (en) 2013-06-06 2021-03-03 Novozymes A/S Alpha-amylase variants and polynucleotides encoding same
WO2014198840A1 (en) 2013-06-12 2014-12-18 Earth Alive Clean Technologies Inc. Dust suppressant
WO2014200657A1 (en) 2013-06-13 2014-12-18 Danisco Us Inc. Alpha-amylase from streptomyces xiamenensis
WO2014200656A1 (en) 2013-06-13 2014-12-18 Danisco Us Inc. Alpha-amylase from streptomyces umbrinus
WO2014200658A1 (en) 2013-06-13 2014-12-18 Danisco Us Inc. Alpha-amylase from promicromonospora vindobonensis
US20160130571A1 (en) 2013-06-17 2016-05-12 Danisco Us Inc. Alpha-Amylase from Bacillaceae Family Member
US20160145596A1 (en) 2013-06-27 2016-05-26 Novozymes A/S Subtilase Variants and Polynucleotides Encoding Same
US10378001B2 (en) 2013-06-27 2019-08-13 Novozymes A/S Subtilase variants and compositions comprising same
KR20160029080A (ko) 2013-07-04 2016-03-14 노보자임스 에이/에스 재오염 방지 효과를 가지는 잔탄 분해효소 활성을 가지는 폴리펩티드들 및 이를 암호화하는 폴리뉴클레오티드들
WO2015004102A1 (en) 2013-07-09 2015-01-15 Novozymes A/S Polypeptides with lipase activity and polynucleotides encoding same
MX371497B (es) 2013-07-19 2020-01-31 Danisco Us Inc Composiciones y metodos que comprenden una variante de enzima lipolitica.
CN117904081A (zh) 2013-07-29 2024-04-19 诺维信公司 蛋白酶变体以及对其进行编码的多核苷酸
EP3613853A1 (en) 2013-07-29 2020-02-26 Novozymes A/S Protease variants and polynucleotides encoding same
EP2832853A1 (en) 2013-07-29 2015-02-04 Henkel AG&Co. KGAA Detergent composition comprising protease variants
EP3052622B1 (en) 2013-10-03 2018-09-19 Danisco US Inc. Alpha-amylases from a subset of exiguobacterium, and methods of use, thereof
WO2015049370A1 (en) 2013-10-03 2015-04-09 Novozymes A/S Detergent composition and use of detergent composition
US20160160199A1 (en) 2013-10-03 2016-06-09 Danisco Us Inc. Alpha-amylases from exiguobacterium, and methods of use, thereof
MX2016006489A (es) 2013-11-20 2016-08-03 Danisco Us Inc Alfa-amilasas variantes que tienen susceptibilidad reducida a la escision por proteasas y metodos de uso.
EP4163305B1 (en) 2013-12-16 2024-07-10 Nutrition & Biosciences USA 4, Inc. Use of poly alpha-1,3-glucan ethers as viscosity modifiers
AU2014364772B2 (en) 2013-12-18 2018-07-26 Nutrition & Biosciences USA 4, Inc. Cationic poly alpha-1,3-glucan ethers
WO2015094809A1 (en) 2013-12-19 2015-06-25 Danisco Us Inc. Chimeric fungal alpha-amylases comprising carbohydrate binding module and the use thereof
CN105814200A (zh) 2013-12-20 2016-07-27 诺维信公司 具有蛋白酶活性的多肽以及编码它们的多核苷酸
WO2015109972A1 (en) 2014-01-22 2015-07-30 Novozymes A/S Polypeptides with lipase activity and polynucleotides encoding same
CN105992796A (zh) 2014-02-14 2016-10-05 纳幕尔杜邦公司 用于粘度调节的聚-α-1,3-1,6-葡聚糖
WO2015134737A1 (en) 2014-03-05 2015-09-11 Novozymes A/S Compositions and methods for improving properties of cellulosic textile materials with xyloglucan endotransglycosylase
CN106062270A (zh) 2014-03-05 2016-10-26 诺维信公司 使用木葡聚糖内糖基转移酶改进非纤维素纺织材料的性质的组合物和方法
EP3116914B8 (en) 2014-03-11 2021-04-21 E. I. du Pont de Nemours and Company Oxidized poly alpha-1,3-glucan as detergent builder
EP3521434A1 (en) 2014-03-12 2019-08-07 Novozymes A/S Polypeptides with lipase activity and polynucleotides encoding same
WO2015150457A1 (en) 2014-04-01 2015-10-08 Novozymes A/S Polypeptides having alpha amylase activity
CN112899086A (zh) 2014-04-11 2021-06-04 诺维信公司 洗涤剂组合物
US10030215B2 (en) 2014-04-15 2018-07-24 Novozymes A/S Polypeptides with lipase activity and polynucleotides encoding same
CN106459937B (zh) 2014-05-27 2024-09-10 诺维信公司 用于产生脂肪酶的方法
WO2015181119A2 (en) 2014-05-27 2015-12-03 Novozymes A/S Lipase variants and polynucleotides encoding same
CN106414729A (zh) 2014-06-12 2017-02-15 诺维信公司 α‑淀粉酶变体以及对其进行编码的多核苷酸
US9714403B2 (en) 2014-06-19 2017-07-25 E I Du Pont De Nemours And Company Compositions containing one or more poly alpha-1,3-glucan ether compounds
EP3919599A1 (en) 2014-06-19 2021-12-08 Nutrition & Biosciences USA 4, Inc. Compositions containing one or more poly alpha-1,3-glucan ether compounds
US20170121646A1 (en) 2014-07-03 2017-05-04 Novozymes A/S Improved Stabilization of Non-Protease Enzyme
EP3327122B1 (en) 2014-07-04 2021-02-17 Novozymes A/S Subtilase variants and polynucleotides encoding same
US10626388B2 (en) 2014-07-04 2020-04-21 Novozymes A/S Subtilase variants and polynucleotides encoding same
CN107207999B (zh) 2014-09-18 2019-09-27 荷兰联合利华有限公司 增白组合物
WO2016079110A2 (en) 2014-11-19 2016-05-26 Novozymes A/S Use of enzyme for cleaning
CN107075489A (zh) 2014-11-20 2017-08-18 诺维信公司 脂环酸芽孢杆菌变体以及编码它们的多核苷酸
CA2963331A1 (en) 2014-12-04 2016-06-09 Novozymes A/S Subtilase variants and polynucleotides encoding same
CN107002054A (zh) 2014-12-05 2017-08-01 诺维信公司 脂肪酶变体以及编码它们的多核苷酸
ES2763235T3 (es) 2014-12-15 2020-05-27 Henkel Ag & Co Kgaa Composición detergente que comprende variantes de subtilasa
WO2016096996A1 (en) 2014-12-16 2016-06-23 Novozymes A/S Polypeptides having n-acetyl glucosamine oxidase activity
WO2016097350A1 (en) 2014-12-19 2016-06-23 Novozymes A/S Protease variants and polynucleotides encoding same
EP3741848A3 (en) 2014-12-19 2021-02-17 Novozymes A/S Protease variants and polynucleotides encoding same
AU2015369965B2 (en) 2014-12-23 2020-01-30 Nutrition & Biosciences USA 4, Inc. Enzymatically produced cellulose
CN107406805A (zh) 2015-01-08 2017-11-28 斯泰潘公司 冷水衣物洗涤剂
WO2016110378A1 (en) 2015-01-09 2016-07-14 Unilever Plc Laundry treatment composition comprising a dye
CN107207998B (zh) 2015-02-13 2020-04-10 荷兰联合利华有限公司 液体洗衣组合物
WO2016133734A1 (en) 2015-02-18 2016-08-25 E. I. Du Pont De Nemours And Company Soy polysaccharide ethers
WO2016160407A1 (en) 2015-03-31 2016-10-06 Stepan Company Detergents based on alpha-sulfonated fatty ester surfactants
WO2016155993A1 (en) 2015-04-02 2016-10-06 Unilever Plc Composition
EP3280818A2 (en) 2015-04-07 2018-02-14 Novozymes A/S Methods for selecting enzymes having lipase activity
WO2016162556A1 (en) 2015-04-10 2016-10-13 Novozymes A/S Laundry method, use of dnase and detergent composition
WO2016162558A1 (en) 2015-04-10 2016-10-13 Novozymes A/S Detergent composition
EP3298121B1 (en) 2015-05-19 2019-03-20 Novozymes A/S Odor reduction
ES2712459T3 (es) 2015-06-04 2019-05-13 Procter & Gamble Composición detergente líquida para lavado de vajillas a mano
EP3287513A1 (en) 2015-06-04 2018-02-28 The Procter & Gamble Company Hand dishwashing liquid detergent composition
WO2016202739A1 (en) 2015-06-16 2016-12-22 Novozymes A/S Polypeptides with lipase activity and polynucleotides encoding same
WO2016202785A1 (en) 2015-06-17 2016-12-22 Novozymes A/S Container
EP3106508B1 (en) 2015-06-18 2019-11-20 Henkel AG & Co. KGaA Detergent composition comprising subtilase variants
EP3310912B1 (en) 2015-06-18 2021-01-27 Novozymes A/S Subtilase variants and polynucleotides encoding same
EP3317388B1 (en) 2015-06-30 2019-11-13 Novozymes A/S Laundry detergent composition, method for washing and use of composition
CN107922930A (zh) 2015-07-01 2018-04-17 诺维信公司 减少气味的方法
EP3950939A3 (en) 2015-07-06 2022-06-08 Novozymes A/S Lipase variants and polynucleotides encoding same
BR112018003766A2 (pt) 2015-08-28 2018-09-25 Unilever Nv composição detergente líquida compreendendo enzima protease e não protease
CN108026487B (zh) 2015-09-17 2021-04-30 汉高股份有限及两合公司 包含具有黄原胶降解活性的多肽的洗涤剂组合物
WO2017046260A1 (en) 2015-09-17 2017-03-23 Novozymes A/S Polypeptides having xanthan degrading activity and polynucleotides encoding same
CN116064474A (zh) 2015-10-07 2023-05-05 诺维信公司 多肽
BR112018007474A2 (pt) 2015-10-14 2018-10-30 Novozymes A/S ?limpeza de membranas de filtração de água?
EP3362556B1 (en) 2015-10-14 2024-07-10 Novozymes A/S Polypeptide variants
EP3362558A1 (en) 2015-10-14 2018-08-22 Novozymes A/S Polypeptides having protease activity and polynucleotides encoding same
MX2018004683A (es) 2015-10-28 2018-07-06 Novozymes As Composicion detergente que comprende variantes de amilasa y proteasa.
JP6997706B2 (ja) 2015-11-13 2022-01-18 ニュートリション・アンド・バイオサイエンシーズ・ユーエスエー・フォー，インコーポレイテッド 洗濯ケアおよび織物ケアにおいて使用するためのグルカン繊維組成物
JP2019504932A (ja) 2015-11-13 2019-02-21 イー・アイ・デュポン・ドウ・ヌムール・アンド・カンパニーＥ．Ｉ．Ｄｕ Ｐｏｎｔ Ｄｅ Ｎｅｍｏｕｒｓ Ａｎｄ Ｃｏｍｐａｎｙ 洗濯ケアおよび織物ケアにおいて使用するためのグルカン繊維組成物
WO2017083228A1 (en) 2015-11-13 2017-05-18 E. I. Du Pont De Nemours And Company Glucan fiber compositions for use in laundry care and fabric care
WO2017089366A1 (en) 2015-11-24 2017-06-01 Novozymes A/S Polypeptides having protease activity and polynucleotides encoding same
US10870838B2 (en) 2015-12-01 2020-12-22 Novozymes A/S Methods for producing lipases
WO2017097866A1 (en) 2015-12-07 2017-06-15 Novozymes A/S Polypeptides having beta-glucanase activity, polynucleotides encoding same and uses thereof in cleaning and detergent compositions
EP4218992A3 (en) 2015-12-09 2023-08-09 Basf Se Method of purifying a protein from fermentation solids under desorbing conditions
DK3387124T3 (da) 2015-12-09 2021-08-23 William Cuevas Kombinatoriske alfa-amylasevarianter
EP3397061A1 (en) 2015-12-28 2018-11-07 Novozymes BioAG A/S Heat priming of bacterial spores
CN108603185B (zh) 2015-12-30 2022-05-17 诺维信公司 酶变体以及编码它们的多核苷酸
EP3190167B1 (en) 2016-01-07 2018-06-06 Unilever PLC Bitter pill
BR112018014327A2 (pt) 2016-01-15 2018-12-11 Unilever Nv composição de tratamento para lavagem e método doméstico de tratamento de um tecido
CA3007148A1 (en) 2016-01-29 2017-08-03 Novozymes A/S Beta-glucanase variants and polynucleotides encoding same
WO2017133879A1 (en) 2016-02-04 2017-08-10 Unilever Plc Detergent liquid
EP3417040B1 (en) 2016-02-17 2019-09-04 Unilever PLC Whitening composition
WO2017140391A1 (en) 2016-02-17 2017-08-24 Unilever Plc Whitening composition
WO2017162378A1 (en) 2016-03-21 2017-09-28 Unilever Plc Laundry detergent composition
US20190048291A1 (en) 2016-03-23 2019-02-14 Novozymes A/S Use of Polypeptide Having DNase Activity for Treating Fabrics
WO2017173190A2 (en) 2016-04-01 2017-10-05 Danisco Us Inc. Alpha-amylases, compositions & methods
WO2017173324A2 (en) 2016-04-01 2017-10-05 Danisco Us Inc. Alpha-amylases, compositions & methods
CN109312270B (zh) 2016-04-08 2022-01-28 诺维信公司 洗涤剂组合物及其用途
WO2017174251A1 (en) 2016-04-08 2017-10-12 Unilever Plc Laundry detergent composition
WO2017186943A1 (en) 2016-04-29 2017-11-02 Novozymes A/S Detergent compositions and uses thereof
EP3452497B1 (en) 2016-05-03 2021-02-17 Novozymes A/S Alpha-amylase variants and polynucleotides encoding the same
EP3455352A1 (en) 2016-05-09 2019-03-20 Novozymes A/S Variant polypeptides with improved performance and use of the same
WO2017210188A1 (en) 2016-05-31 2017-12-07 Novozymes A/S Stabilized liquid peroxide compositions
WO2017207762A1 (en) 2016-06-03 2017-12-07 Novozymes A/S Subtilase variants and polynucleotides encoding same
WO2017211697A1 (en) 2016-06-09 2017-12-14 Unilever Plc Laundry products
US11001787B2 (en) 2016-06-23 2021-05-11 Novozymes A/S Use of enzymes, composition and method for removing soil
CN117721095A (zh) 2016-06-30 2024-03-19 诺维信公司 脂肪酶变体及包含表面活性剂和脂肪酶变体的组合物
WO2018002261A1 (en) 2016-07-01 2018-01-04 Novozymes A/S Detergent compositions
WO2018007573A1 (en) 2016-07-08 2018-01-11 Novozymes A/S Detergent compositions with galactanase
CN109642222A (zh) 2016-07-13 2019-04-16 诺维信公司 食物芽孢杆菌dna酶变体
WO2018011242A1 (en) 2016-07-14 2018-01-18 Basf Se Fermentation medium comprising chelating agent
US11326152B2 (en) 2016-07-18 2022-05-10 Novozymes A/S Lipase variants, polynucleotides encoding same and the use thereof
EP3284805B1 (en) 2016-08-17 2020-02-19 The Procter & Gamble Company Cleaning composition comprising enzymes
KR102507692B1 (ko) 2016-08-24 2023-03-09 헨켈 아게 운트 코. 카게아아 Gh9 엔도글루카나제 변이체 i을 포함하는 세제 조성물
AU2017317563B8 (en) 2016-08-24 2023-03-23 Henkel Ag & Co. Kgaa Detergent compositions comprising xanthan lyase variants I
WO2018037062A1 (en) 2016-08-24 2018-03-01 Novozymes A/S Gh9 endoglucanase variants and polynucleotides encoding same
CN109844110B (zh) 2016-08-24 2023-06-06 诺维信公司 黄原胶裂解酶变体以及编码它们的多核苷酸
CN109996859B (zh) 2016-09-29 2021-11-30 诺维信公司 含孢子的颗粒
WO2018060216A1 (en) 2016-09-29 2018-04-05 Novozymes A/S Use of enzyme for washing, method for washing and warewashing composition
BR112019007851B1 (pt) 2016-10-18 2022-10-18 Unilever Ip Holdings B.V. Composição detergente para lavagem de roupas e método doméstico de tratamento de um tecido
WO2018077938A1 (en) 2016-10-25 2018-05-03 Novozymes A/S Detergent compositions
US11753605B2 (en) 2016-11-01 2023-09-12 Novozymes A/S Multi-core granules
WO2018099762A1 (en) 2016-12-01 2018-06-07 Basf Se Stabilization of enzymes in compositions
WO2018108865A1 (en) 2016-12-12 2018-06-21 Novozymes A/S Use of polypeptides
EP3555255B1 (en) 2016-12-15 2020-06-24 Unilever PLC Laundry detergent composition
US11149233B2 (en) 2017-03-31 2021-10-19 Novozymes A/S Polypeptides having RNase activity
EP3601550A1 (en) 2017-03-31 2020-02-05 Novozymes A/S Polypeptides having dnase activity
JP2020515269A (ja) 2017-03-31 2020-05-28 ダニスコ・ユーエス・インク α−アミラーゼ組み合わせ変異体
US11053483B2 (en) 2017-03-31 2021-07-06 Novozymes A/S Polypeptides having DNase activity
EP3607040A1 (en) 2017-04-04 2020-02-12 Novozymes A/S Polypeptide compositions and uses thereof
WO2018185150A1 (en) 2017-04-04 2018-10-11 Novozymes A/S Polypeptides
CN110651029B (zh) 2017-04-04 2022-02-15 诺维信公司 糖基水解酶
EP3385362A1 (en) 2017-04-05 2018-10-10 Henkel AG & Co. KGaA Detergent compositions comprising fungal mannanases
DK3385361T3 (da) 2017-04-05 2019-06-03 Ab Enzymes Gmbh Detergentsammensætninger omfattende bakterielle mannanaser
WO2018185280A1 (en) 2017-04-06 2018-10-11 Novozymes A/S Cleaning compositions and uses thereof
WO2018185269A1 (en) 2017-04-06 2018-10-11 Novozymes A/S Cleaning compositions and uses thereof
EP3967756A1 (en) 2017-04-06 2022-03-16 Novozymes A/S Detergent compositions and uses thereof
WO2018184818A1 (en) 2017-04-06 2018-10-11 Novozymes A/S Cleaning compositions and uses thereof
WO2018185267A1 (en) 2017-04-06 2018-10-11 Novozymes A/S Cleaning compositions and uses thereof
EP3607038A1 (en) 2017-04-06 2020-02-12 Novozymes A/S Cleaning compositions and uses thereof
EP3607037A1 (en) 2017-04-06 2020-02-12 Novozymes A/S Cleaning compositions and uses thereof
WO2018185285A1 (en) 2017-04-06 2018-10-11 Novozymes A/S Cleaning compositions and uses thereof
WO2018202846A1 (en) 2017-05-05 2018-11-08 Novozymes A/S Compositions comprising lipase and sulfite
WO2018206535A1 (en) 2017-05-08 2018-11-15 Novozymes A/S Carbohydrate-binding domain and polynucleotides encoding the same
CN110662837B (zh) 2017-05-08 2024-04-12 诺维信公司 甘露聚糖酶变体和对其编码的多核苷酸
WO2018206302A1 (en) 2017-05-08 2018-11-15 Novozymes A/S Mannanase variants and polynucleotides encoding same
EP3401385A1 (en) 2017-05-08 2018-11-14 Henkel AG & Co. KGaA Detergent composition comprising polypeptide comprising carbohydrate-binding domain
WO2018224544A1 (en) 2017-06-08 2018-12-13 Novozymes A/S Compositions comprising polypeptides having cellulase activity and amylase activity, and uses thereof in cleaning and detergent compositions
EP3642319B1 (en) 2017-06-20 2020-12-30 Unilever N.V. Particulate detergent composition comprising perfume
WO2018234003A1 (en) 2017-06-21 2018-12-27 Unilever Plc PACKAGING AND DISTRIBUTION OF DETERGENT COMPOSITIONS
EP3645692A1 (en) 2017-06-30 2020-05-06 Novozymes A/S Enzyme slurry composition
CN110892053A (zh) 2017-07-07 2020-03-17 荷兰联合利华有限公司 洗衣清洁组合物
CN110869480B (zh) 2017-07-07 2021-08-13 联合利华知识产权控股有限公司 增白组合物
EP3665267A1 (en) 2017-08-07 2020-06-17 Novozymes A/S Use of fca control based on ph
US11441139B2 (en) 2017-08-18 2022-09-13 Danisco Us Inc (157111) α-Amylase variants
CA3070749A1 (en) 2017-08-24 2019-02-28 Novozymes A/S Gh9 endoglucanase variants and polynucleotides encoding same
WO2019038187A1 (en) 2017-08-24 2019-02-28 Unilever Plc IMPROVEMENTS RELATING TO THE CLEANING OF FABRICS
WO2019038059A1 (en) 2017-08-24 2019-02-28 Henkel Ag & Co. Kgaa DETERGENT COMPOSITIONS COMPRISING GH9 ENDOGLUCANASE VARIANTS II
WO2019038186A1 (en) 2017-08-24 2019-02-28 Unilever Plc IMPROVEMENTS RELATING TO THE CLEANING OF FABRICS
CA3071078A1 (en) 2017-08-24 2019-02-28 Novozymes A/S Xanthan lyase variants and polynucleotides encoding same
EP3673060A1 (en) 2017-08-24 2020-07-01 Henkel AG & Co. KGaA Detergent composition comprising xanthan lyase variants ii
US20200277553A1 (en) 2017-09-20 2020-09-03 Novozymes A/S Use of Enzymes for Improving Water Absorption And/Or Whiteness
US11414814B2 (en) 2017-09-22 2022-08-16 Novozymes A/S Polypeptides
MX2020003779A (es) 2017-09-27 2020-08-03 Procter & Gamble Composiciones detergentes que comprenden lipasas.
US11332725B2 (en) 2017-09-27 2022-05-17 Novozymes A/S Lipase variants and microcapsule compositions comprising such lipase variants
EP3692148A1 (en) 2017-10-02 2020-08-12 Novozymes A/S Polypeptides having mannanase activity and polynucleotides encoding same
CN111417725A (zh) 2017-10-02 2020-07-14 诺维信公司 具有甘露聚糖酶活性的多肽和编码它们的多核苷酸
WO2019076834A1 (en) 2017-10-16 2019-04-25 Novozymes A/S PELLETS WITH LOW DUST CONTENT
WO2019076800A1 (en) 2017-10-16 2019-04-25 Novozymes A/S CLEANING COMPOSITIONS AND USES THEREOF
US20230193162A1 (en) 2017-10-16 2023-06-22 Novozymes A/S Low dusting granules
EP3701001A1 (en) 2017-10-24 2020-09-02 Novozymes A/S Compositions comprising polypeptides having mannanase activity
HUE057471T2 (hu) 2017-10-27 2022-05-28 Procter & Gamble Polipeptid-variánsokat tartalmazó mosószerkészítmények
CN111542604A (zh) 2017-10-27 2020-08-14 诺维信公司 Dna酶变体
DE102017125560A1 (de) 2017-11-01 2019-05-02 Henkel Ag & Co. Kgaa Reinigungszusammensetzungen, die dispersine iii enthalten
US20200291330A1 (en) 2017-11-01 2020-09-17 Novozymes A/S Polypeptides and Compositions Comprising Such Polypeptides
US11505767B2 (en) 2017-11-01 2022-11-22 Novozymes A/S Methods for cleansing medical devices
DE102017125558A1 (de) 2017-11-01 2019-05-02 Henkel Ag & Co. Kgaa Reinigungszusammensetzungen, die dispersine i enthalten
BR112020008737A2 (pt) 2017-11-01 2020-10-13 Novozymes A/S polipeptídeos e composições que compreendam tais polipeptídeos
DE102017125559A1 (de) 2017-11-01 2019-05-02 Henkel Ag & Co. Kgaa Reinigungszusammensetzungen, die dispersine ii enthalten
EP3707255A1 (en) 2017-11-09 2020-09-16 Basf Se Coatings of enzyme particles comprising organic white pigments
WO2019101951A1 (en) 2017-11-24 2019-05-31 Novozymes A/S Small enzyme particles for interesterification
WO2019105780A1 (en) 2017-11-29 2019-06-06 Basf Se Compositions, their manufacture and use
EP3717616B1 (en) 2017-11-30 2021-10-13 Unilever IP Holdings B.V. Detergent composition comprising protease
WO2019110462A1 (en) 2017-12-04 2019-06-13 Novozymes A/S Lipase variants and polynucleotides encoding same
WO2019121057A1 (en) 2017-12-20 2019-06-27 Basf Se Laundry formulation for removing fatty compounds having a melting temperature>30°c deposited on textiles
CN111757930A (zh) 2018-02-08 2020-10-09 诺维信公司 脂肪酶变体及其组合物
US20210123033A1 (en) 2018-02-08 2021-04-29 Novozymes A/S Lipases, Lipase Variants and Compositions Thereof
WO2019162000A1 (en) 2018-02-23 2019-08-29 Henkel Ag & Co. Kgaa Detergent composition comprising xanthan lyase and endoglucanase variants
US20210002588A1 (en) 2018-03-13 2021-01-07 Novozymes A/S Microencapsulation Using Amino Sugar Oligomers
EP3768835A1 (en) 2018-03-23 2021-01-27 Novozymes A/S Subtilase variants and compositions comprising same
US11535837B2 (en) 2018-03-29 2022-12-27 Novozymes A/S Mannanase variants and polynucleotides encoding same
US20210009927A1 (en) 2018-04-17 2021-01-14 Novozymes A/S Polypeptides Comprising Carbohydrate Binding Activity in Detergent Compositions And Their use in Reducing Wrinkles in Textile or Fabrics
CN111989389A (zh) 2018-04-19 2020-11-24 巴斯夫欧洲公司 组合物以及可用于该组合物的聚合物
EP3781679A1 (en) 2018-04-19 2021-02-24 Novozymes A/S Stabilized cellulase variants
WO2019201785A1 (en) 2018-04-19 2019-10-24 Novozymes A/S Stabilized cellulase variants
BR112020023123A2 (pt) 2018-05-17 2021-02-02 Unilever N.V. composição de limpeza e método doméstico para tratar um tecido
CN112119147B (zh) 2018-05-17 2023-09-29 联合利华知识产权控股有限公司 清洁组合物
WO2019238761A1 (en) 2018-06-15 2019-12-19 Basf Se Water soluble multilayer films containing wash active chemicals and enzymes
EP3814472A1 (en) 2018-06-28 2021-05-05 Novozymes A/S Detergent compositions and uses thereof
EP3814489A1 (en) 2018-06-29 2021-05-05 Novozymes A/S Subtilase variants and compositions comprising same
US20210071116A1 (en) 2018-06-29 2021-03-11 Novozymes A/S Detergent Compositions and Uses Thereof
CN112352039B (zh) 2018-07-02 2022-11-15 诺维信公司 清洁组合物及其用途
US20210301223A1 (en) 2018-07-03 2021-09-30 Novozymes A/S Cleaning compositions and uses thereof
WO2020008043A1 (en) 2018-07-06 2020-01-09 Novozymes A/S Cleaning compositions and uses thereof
WO2020008024A1 (en) 2018-07-06 2020-01-09 Novozymes A/S Cleaning compositions and uses thereof
EP3824057B1 (en) 2018-07-17 2023-10-18 Unilever Global IP Limited Use of a rhamnolipid in a surfactant system
EP3775137A1 (en) 2018-07-27 2021-02-17 Unilever N.V. Laundry detergent
CA3108284A1 (en) 2018-07-31 2020-02-06 Danisco Us Inc Variant alpha-amylases having amino acid substitutions that lower the pka of the general acid
WO2020030623A1 (en) 2018-08-10 2020-02-13 Basf Se Packaging unit comprising a detergent composition containing an enzyme and at least one chelating agent
US20210340466A1 (en) 2018-10-01 2021-11-04 Novozymes A/S Detergent compositions and uses thereof
EP3861094A1 (en) 2018-10-02 2021-08-11 Novozymes A/S Cleaning composition
WO2020070014A1 (en) 2018-10-02 2020-04-09 Novozymes A/S Cleaning composition comprising anionic surfactant and a polypeptide having rnase activity
WO2020070209A1 (en) 2018-10-02 2020-04-09 Novozymes A/S Cleaning composition
WO2020070249A1 (en) 2018-10-03 2020-04-09 Novozymes A/S Cleaning compositions
CN113056476A (zh) 2018-10-03 2021-06-29 诺维信公司 具有α-甘露聚糖降解活性的多肽以及编码它们的多核苷酸
CN112840021A (zh) 2018-10-05 2021-05-25 巴斯夫欧洲公司 在液体中稳定水解酶的化合物
MX2021003931A (es) 2018-10-05 2021-06-04 Basf Se Compuestos estabilizadores de amilasas en liquidos.
EP3677676A1 (en) 2019-01-03 2020-07-08 Basf Se Compounds stabilizing amylases in liquids
BR112021005954A2 (pt) 2018-10-05 2021-06-29 Basf Se preparação enzimática, processo para produzir uma preparação enzimática estável, métodos para reduzir perda de atividade lipolítica de pelo menos uma lipase, para preparar uma formulação de detergente, para remover manchas e para aumentar a estabilidade de armazenagem de uma formulação de detergente líquido, usos de um composto e da preparação enzimática, e, formulação de detergente.
US20220033739A1 (en) 2018-10-11 2022-02-03 Novozymes A/S Cleaning compositions and uses thereof
CN113166745A (zh) 2018-10-12 2021-07-23 丹尼斯科美国公司 在螯合剂存在下具有可增强稳定性的突变的α-淀粉酶
ES2981999T3 (es) 2018-10-31 2024-10-14 Henkel Ag & Co Kgaa Composiciones limpiadoras que contienen dispersinas V
EP3647397A1 (en) 2018-10-31 2020-05-06 Henkel AG & Co. KGaA Cleaning compositions containing dispersins iv
WO2020104231A1 (en) 2018-11-19 2020-05-28 Basf Se Powders and granules containing a chelating agent and an enzyme
EP3884023B1 (en) 2018-11-20 2024-07-17 Unilever Global Ip Limited Detergent composition
WO2020104157A1 (en) 2018-11-20 2020-05-28 Unilever Plc Detergent composition
CN113056549B (zh) 2018-11-20 2023-03-10 联合利华知识产权控股有限公司 洗涤剂组合物
CN113056550B (zh) 2018-11-20 2022-10-28 联合利华知识产权控股有限公司 洗涤剂组合物
EP3891277A1 (en) 2018-12-03 2021-10-13 Novozymes A/S Powder detergent compositions
WO2020114965A1 (en) 2018-12-03 2020-06-11 Novozymes A/S LOW pH POWDER DETERGENT COMPOSITION
WO2020127775A1 (en) 2018-12-21 2020-06-25 Novozymes A/S Detergent pouch comprising metalloproteases
EP3898962A2 (en) 2018-12-21 2021-10-27 Novozymes A/S Polypeptides having peptidoglycan degrading activity and polynucleotides encoding same
BR112021014336A2 (pt) 2019-01-22 2021-09-21 Unilever Ip Holdings B.V. Composição detergente de lavanderia, método doméstico para tratar um tecido e corante tonalizante não leuco azul ou violeta
BR112021014332A2 (pt) 2019-01-22 2021-09-28 Unilever Ip Holdings B.V. Composição detergente de lavanderia, método doméstico para tratar um tecido e corante tonalizante não leuco azul ou violeta
EP3702452A1 (en) 2019-03-01 2020-09-02 Novozymes A/S Detergent compositions comprising two proteases
US20220177808A1 (en) 2019-03-08 2022-06-09 Basf Se Cationic surfactant and its use in laundry detergent compositions
WO2020188095A1 (en) 2019-03-21 2020-09-24 Novozymes A/S Alpha-amylase variants and polynucleotides encoding same
CN113785039B (zh) 2019-04-03 2024-06-18 诺维信公司 具有β-葡聚糖酶活性的多肽、编码其的多核苷酸及其在清洁和洗涤剂组合物中的用途
EP3953462A1 (en) 2019-04-10 2022-02-16 Novozymes A/S Polypeptide variants
US20220186151A1 (en) 2019-04-12 2022-06-16 Novozymes A/S Stabilized glycoside hydrolase variants
WO2020229480A1 (en) 2019-05-14 2020-11-19 Basf Se Compounds stabilizing hydrolases in liquids
CN113825829A (zh) 2019-05-16 2021-12-21 联合利华知识产权控股有限公司 洗衣组合物
EP3744838A1 (en) 2019-05-29 2020-12-02 Novozymes A/S Lipolytic polymer particles for esterification and interesterification
WO2020260006A1 (en) 2019-06-28 2020-12-30 Unilever Plc Detergent compositions
US20220364020A1 (en) 2019-06-28 2022-11-17 Conopco, Inc., D/B/A Unilever Detergent composition
US20220372400A1 (en) 2019-06-28 2022-11-24 Conopco, Inc., D/B/A Unilever Detergent composition
EP3990604B1 (en) 2019-06-28 2022-12-14 Unilever Global IP Limited Detergent composition
BR112021025261A2 (pt) 2019-06-28 2022-04-26 Unilever Ip Holdings B V Composição detergente e método doméstico para tratar um tecido
WO2021001244A1 (en) 2019-07-01 2021-01-07 Basf Se Peptide acetals for stabilising enzymes
EP3994255A1 (en) 2019-07-02 2022-05-11 Novozymes A/S Lipase variants and compositions thereof
US20220403298A1 (en) 2019-07-12 2022-12-22 Novozymes A/S Enzymatic emulsions for detergents
WO2021037895A1 (en) 2019-08-27 2021-03-04 Novozymes A/S Detergent composition
US20240294852A1 (en) 2019-08-27 2024-09-05 Novozymes A/S Composition comprising a lipase
BR112022003050A2 (pt) 2019-09-02 2022-05-17 Unilever Ip Holdings B V Composição detergente de lavagem de roupas aquosa e método doméstico para tratar um tecido
CN114616312A (zh) 2019-09-19 2022-06-10 诺维信公司 洗涤剂组合物
EP4038170A1 (en) 2019-10-03 2022-08-10 Novozymes A/S Polypeptides comprising at least two carbohydrate binding domains
AR120142A1 (es) 2019-10-07 2022-02-02 Unilever Nv Composición detergente
EP4045625A1 (en) 2019-10-18 2022-08-24 Basf Se Storage-stable hydrolase containing liquids
BR112022007697A2 (pt) 2019-10-24 2022-07-12 Danisco Us Inc Alfa-amilase variante que forma maltopentaose/maltohexaose
WO2021105336A1 (en) 2019-11-29 2021-06-03 Basf Se Compositions comprising polymer and enzyme
WO2021115912A1 (en) 2019-12-09 2021-06-17 Basf Se Formulations comprising a hydrophobically modified polyethyleneimine and one or more enzymes
WO2021121394A1 (en) 2019-12-20 2021-06-24 Novozymes A/S Stabilized liquid boron-free enzyme compositions
CN114846128A (zh) 2019-12-20 2022-08-02 汉高股份有限及两合公司 包含分散蛋白viii的清洁组合物
KR20220121235A (ko) 2019-12-20 2022-08-31 헨켈 아게 운트 코. 카게아아 디스페르신 및 카르보히드라제를 포함하는 세정 조성물
AU2020405786A1 (en) 2019-12-20 2022-08-11 Henkel Ag & Co. Kgaa Cleaning compositions comprising dispersins IX
US20230048546A1 (en) 2019-12-20 2023-02-16 Henkel Ag & Co. Kgaa Cleaning compositions comprising dispersins vi
US20220411773A1 (en) 2019-12-20 2022-12-29 Novozymes A/S Polypeptides having proteolytic activity and use thereof
EP4097226A1 (en) 2020-01-31 2022-12-07 Novozymes A/S Mannanase variants and polynucleotides encoding same
JP2023511739A (ja) 2020-01-31 2023-03-22 ノボザイムス アクティーゼルスカブ マンナナーゼバリアント及びそれをコードするポリヌクレオチド
EP3892708A1 (en) 2020-04-06 2021-10-13 Henkel AG & Co. KGaA Cleaning compositions comprising dispersin variants
WO2021204838A1 (en) 2020-04-08 2021-10-14 Novozymes A/S Carbohydrate binding module variants
US20230167384A1 (en) 2020-04-21 2023-06-01 Novozymes A/S Cleaning compositions comprising polypeptides having fructan degrading activity
EP3907271A1 (en) 2020-05-07 2021-11-10 Novozymes A/S Cleaning composition, use and method of cleaning
US20230212548A1 (en) 2020-05-26 2023-07-06 Novozymes A/S Subtilase variants and compositions comprising same
CN115698246A (zh) 2020-06-08 2023-02-03 联合利华知识产权控股有限公司 提高蛋白酶活性的方法
CN115516072A (zh) 2020-06-18 2022-12-23 巴斯夫欧洲公司 组合物及其用途
WO2021259099A1 (en) 2020-06-24 2021-12-30 Novozymes A/S Use of cellulases for removing dust mite from textile
EP3936593A1 (en) 2020-07-08 2022-01-12 Henkel AG & Co. KGaA Cleaning compositions and uses thereof
BR112023000148A2 (pt) 2020-07-09 2023-01-31 Basf Se Composição, uso de uma composição, polímero, processo para fazer polímeros, e, método para melhorar o desempenho de limpeza de uma composição detergente líquida
WO2022008732A1 (en) 2020-07-10 2022-01-13 Basf Se Enhancing the activity of antimicrobial preservatives
CN116057158A (zh) 2020-07-27 2023-05-02 联合利华知识产权控股有限公司 酶和表面活性剂用于抑制微生物的用途
JP2023538740A (ja) 2020-08-25 2023-09-11 ノボザイムス アクティーゼルスカブ ファミリー４４キシログルカナーゼの変異体
BR112023001773A2 (pt) 2020-08-28 2023-03-28 Unilever Ip Holdings B V Composição detergente e método
WO2022043042A1 (en) 2020-08-28 2022-03-03 Unilever Ip Holdings B.V. Detergent composition
BR112023002979A2 (pt) 2020-08-28 2023-04-04 Unilever Ip Holdings B V Composição detergente e método de tratamento de um artigo têxtil
US20230323330A1 (en) 2020-08-28 2023-10-12 Novozymes A/S Polyester degrading protease variants
BR112023002833A2 (pt) 2020-08-28 2023-03-14 Unilever Ip Holdings B V Composição detergente e método de tratamento de um artigo têxtil
BR112023003008A2 (pt) 2020-08-28 2023-04-04 Unilever Ip Holdings B V Tensoativo de sulfonato de alcano secundário (sas), composição detergente e método de tratamento de um artigo têxtil
CN116096731A (zh) 2020-09-22 2023-05-09 巴斯夫欧洲公司 蛋白酶和蛋白酶抑制剂与第二酶的改进的组合
EP4225905A2 (en) 2020-10-07 2023-08-16 Novozymes A/S Alpha-amylase variants
WO2022083949A1 (en) 2020-10-20 2022-04-28 Basf Se Compositions and their use
WO2022084303A2 (en) 2020-10-20 2022-04-28 Novozymes A/S Use of polypeptides having dnase activity
WO2022090320A1 (en) 2020-10-28 2022-05-05 Novozymes A/S Use of lipoxygenase
BR112023008326A2 (pt) 2020-10-29 2023-12-12 Novozymes As Variantes de lipase e composições compreendendo tais variantes de lipase
WO2022103725A1 (en) 2020-11-13 2022-05-19 Novozymes A/S Detergent composition comprising a lipase
WO2022106400A1 (en) 2020-11-18 2022-05-27 Novozymes A/S Combination of immunochemically different proteases
WO2022106404A1 (en) 2020-11-18 2022-05-27 Novozymes A/S Combination of proteases
US20240002751A1 (en) 2020-12-17 2024-01-04 Conopco, Inc., D/B/A Unilever Cleaning composition
WO2022128786A1 (en) 2020-12-17 2022-06-23 Unilever Ip Holdings B.V. Use and cleaning composition
EP4032966A1 (en) 2021-01-22 2022-07-27 Novozymes A/S Liquid enzyme composition with sulfite scavenger
EP4284905A1 (en) 2021-01-28 2023-12-06 Novozymes A/S Lipase with low malodor generation
EP4039806A1 (en) 2021-02-04 2022-08-10 Henkel AG & Co. KGaA Detergent composition comprising xanthan lyase and endoglucanase variants with im-proved stability
CN117015592A (zh) 2021-02-12 2023-11-07 诺维信公司 稳定的生物洗涤剂
CN116829709A (zh) 2021-02-12 2023-09-29 诺维信公司 α-淀粉酶变体
KR20230147071A (ko) 2021-02-22 2023-10-20 헨켈 아게 운트 코. 카게아아 아밀라제 변이체
EP4047088A1 (en) 2021-02-22 2022-08-24 Basf Se Amylase variants
EP4305146A1 (en) 2021-03-12 2024-01-17 Novozymes A/S Polypeptide variants
US20240060061A1 (en) 2021-03-15 2024-02-22 Novozymes A/S Dnase variants
EP4060036A1 (en) 2021-03-15 2022-09-21 Novozymes A/S Polypeptide variants
US20240218300A1 (en) 2021-03-26 2024-07-04 Novozymes A/S Detergent composition with reduced polymer content
WO2022268885A1 (en) 2021-06-23 2022-12-29 Novozymes A/S Alpha-amylase polypeptides
EP4405450A1 (en) 2021-09-20 2024-07-31 Unilever IP Holdings B.V. Detergent composition
CN118202030A (zh) 2021-10-13 2024-06-14 巴斯夫欧洲公司 包含聚合物的组合物、聚合物及其用途
WO2023088777A1 (en) 2021-11-22 2023-05-25 Basf Se Compositions comprising polymers, polymers, and their use
CN118647716A (zh) 2021-12-16 2024-09-13 丹尼斯科美国公司 成麦芽五糖/麦芽六糖变体α-淀粉酶
WO2023117992A1 (en) 2021-12-21 2023-06-29 Basf Se Environmental attributes for aqueous polymeric composition
CN118871559A (zh) 2021-12-21 2024-10-29 诺维信公司 包含脂肪酶和加强剂的组合物
EP4206309A1 (en) 2021-12-30 2023-07-05 Novozymes A/S Protein particles with improved whiteness
MX2024009542A (es) 2022-02-04 2024-08-19 Basf Se Composiciones que comprenden polimeros, polimeros y su uso.
EP4234664A1 (en) 2022-02-24 2023-08-30 Evonik Operations GmbH Composition comprising glucolipids and enzymes
WO2023165507A1 (en) 2022-03-02 2023-09-07 Novozymes A/S Use of xyloglucanase for improvement of sustainability of detergents
WO2023165950A1 (en) 2022-03-04 2023-09-07 Novozymes A/S Dnase variants and compositions
AU2023250091A1 (en) 2022-04-08 2024-10-03 Novozymes A/S Hexosaminidase variants and compositions
WO2023227356A1 (en) 2022-05-27 2023-11-30 Unilever Ip Holdings B.V. Composition containing enzyme
DE102022205591A1 (de) 2022-06-01 2023-12-07 Henkel Ag & Co. Kgaa Wasch- und reinigungsmittel mit verbesserter enzymstabilität
DE102022205593A1 (de) 2022-06-01 2023-12-07 Henkel Ag & Co. Kgaa Wasch- und reinigungsmittel mit verbesserter enzymstabilität
DE102022205588A1 (de) 2022-06-01 2023-12-07 Henkel Ag & Co. Kgaa Wasch- und reinigungsmittel mit verbesserter enzymstabilität
WO2023247348A1 (en) 2022-06-21 2023-12-28 Novozymes A/S Mannanase variants and polynucleotides encoding same
WO2023247664A2 (en) 2022-06-24 2023-12-28 Novozymes A/S Lipase variants and compositions comprising such lipase variants
WO2024033135A2 (en) 2022-08-11 2024-02-15 Basf Se Amylase variants
WO2024033134A1 (en) 2022-08-11 2024-02-15 Basf Se Enzyme compositions comprising protease, mannanase, and/or cellulase
WO2024033136A1 (en) 2022-08-11 2024-02-15 Basf Se Amylase variants
WO2024033133A2 (en) 2022-08-11 2024-02-15 Basf Se Enzyme compositions comprising an amylase
EP4324900A1 (en) 2022-08-17 2024-02-21 Henkel AG & Co. KGaA Detergent composition comprising enzymes
WO2024083589A1 (en) 2022-10-18 2024-04-25 Basf Se Detergent compositions, polymers and methods of manufacturing the same
WO2024094732A1 (en) 2022-11-04 2024-05-10 Basf Se Polypeptides having protease activity for use in detergent compositions
WO2024094733A1 (en) 2022-11-04 2024-05-10 Basf Se Polypeptides having protease activity for use in detergent compositions
WO2024094735A1 (en) 2022-11-04 2024-05-10 Basf Se Polypeptides having protease activity for use in detergent compositions
DE102022131732A1 (de) 2022-11-30 2024-06-06 Henkel Ag & Co. Kgaa Verbesserte Waschleistung durch den Einsatz einer Protease fusioniert mit speziellem Adhäsionsvermittlerpeptid
WO2024115754A1 (en) 2022-12-02 2024-06-06 Basf Se Aqueous compositions containing polyalkoxylates, polyalkoxylates, and use
WO2024121058A1 (en) 2022-12-05 2024-06-13 Novozymes A/S A composition comprising a lipase and a peptide
WO2024121070A1 (en) 2022-12-05 2024-06-13 Novozymes A/S Protease variants and polynucleotides encoding same
WO2024126483A1 (en) 2022-12-14 2024-06-20 Novozymes A/S Improved lipase (gcl1) variants
EP4389864A1 (en) 2022-12-20 2024-06-26 Basf Se Cutinases
WO2024131880A2 (en) 2022-12-23 2024-06-27 Novozymes A/S Detergent composition comprising catalase and amylase
WO2024156628A1 (en) 2023-01-23 2024-08-02 Novozymes A/S Cleaning compositions and uses thereof
WO2024183958A1 (en) 2023-03-09 2024-09-12 Norfalk Aps Use of mono-ester glycolipids in laundry detergents
WO2024194245A1 (en) 2023-03-21 2024-09-26 Novozymes A/S Detergent compositions based on biosurfactants
WO2024213513A1 (en) 2023-04-12 2024-10-17 Novozymes A/S Compositions comprising polypeptides having alkaline phosphatase activity

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1987000859A1 (en) * 1985-08-09 1987-02-12 Gist-Brocades N.V. Novel lipolytic enzymes and their use in detergent compositions
GB8915658D0 (en) * 1989-07-07 1989-08-23 Unilever Plc Enzymes,their production and use
DK97190D0 (da) * 1990-04-19 1990-04-19 Novo Nordisk As Oxidationsstabile detergentenzymer

Also Published As

Publication number Publication date
EP0652946B1 (en) 2005-01-26
FI116728B (fi) 2006-02-15
FI945998A (fi) 1995-02-10
JP3618748B2 (ja) 2005-02-09
DE69434242T2 (de) 2006-01-12
DE69434242D1 (de) 2005-03-03
WO1994025578A1 (en) 1994-11-10
CA2138519A1 (en) 1994-11-10
PL306812A1 (en) 1995-04-18
DK0652946T3 (da) 2005-05-30
JPH07508659A (ja) 1995-09-28
ATE287946T1 (de) 2005-02-15
EP0652946A1 (en) 1995-05-17
CA2138519C (en) 2007-06-12
AU6723394A (en) 1994-11-21
AU673078B2 (en) 1996-10-24
KR950702240A (ko) 1995-06-19
FI945998A0 (fi) 1994-12-21

Similar Documents

Publication Publication Date Title
CN1108457A (zh) 用于洗涤剂领域的新的脂酶变异体
DE69632538T2 (de) Neuartige lipolytische enzyme
DE69226182T2 (de) Stabilisierte enzyme und waschmittelzusammensetzungen
US8772007B2 (en) Perhydrolase
CN109312271A (zh) 洗涤剂组合物及其用途
WO2019086521A1 (en) Cleaning compositions containing dispersins ii
KR20200071133A (ko) 디스페르신 iii을 함유하는 세정 조성물
US20240034960A1 (en) Enzymes and enzyme compositions for cleaning
EP2290061A2 (en) Subtilase variants
CN1177376A (zh) 新的脂酶基因和用该基因产生脂酶的方法
CN107002057A (zh) 包括蛋白酶变体的液体清洁组合物
MX2012003386A (es) Variantes de subtilasa.
CN1906303A (zh) 丝氨酸蛋白酶、编码丝氨酸酶的核酸以及包含它们的载体和宿主细胞
CN1147836A (zh) 制备脂解酶变异体的方法
CN1124039A (zh) 脂酶变异体
US20240150738A1 (en) Variant enzymes and uses thereof
SK284609B6 (sk) Detergentná zmes obsahujúca subtilázový BLS309 variant
CN114174486A (zh) 用于清洁的方法和组合物
US20240034961A1 (en) Enzymes and enzyme compositions for cleaning
WO2023278297A1 (en) Variant lipases and uses thereof
US9359584B2 (en) Microbial enzymes as detergent additives
CN86106566A (zh) 新的脂解酶及其在除垢组合物中的应用
WO2023168234A1 (en) Enzymes and enzyme compositions for cleaning
CN106715465A (zh) 具有脂肪酶活性的多肽和编码它们的多核苷酸

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
C53 Correction of patent of invention or patent application
COR Change of bibliographic data

Free format text: CORRECT: APPLICANT; FROM: JIQITE BROCARDIS CO., LTD. TO: JINKEKE INTERNATIONAL CO., LTD.

CP03 Change of name, title or address

Address after: American New York

Applicant after: Genencor International Co., Ltd.

Address before: Netherlands

Applicant before: The wedding day Tebuluo Cadiz Limited by Share Ltd

AD01 Patent right deemed abandoned
C20 Patent right or utility model deemed to be abandoned or is abandoned