DK2825643T3 - Variant-alfa-amylaser med forbedret aktivitet over for stivelsespolymerer - Google Patents

Variant-alfa-amylaser med forbedret aktivitet over for stivelsespolymerer Download PDF

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Publication number
DK2825643T3
DK2825643T3 DK13728877.5T DK13728877T DK2825643T3 DK 2825643 T3 DK2825643 T3 DK 2825643T3 DK 13728877 T DK13728877 T DK 13728877T DK 2825643 T3 DK2825643 T3 DK 2825643T3
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Denmark
Prior art keywords
amylases
activity against
enhanced activity
starch polymers
variant alpha
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DK13728877.5T
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English (en)
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DK2825643T1 (da
Inventor
Richard R Bott
Luis G Cascao-Pereira
David A Estell
Marc Kolkman
David E Wildes
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Danisco Us Inc
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Publication of DK2825643T1 publication Critical patent/DK2825643T1/da
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/24Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405Glucanases
    • C12N9/2408Glucanases acting on alpha -1,4-glucosidic bonds
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/24Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405Glucanases
    • C12N9/2408Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411Amylases
    • C12N9/2414Alpha-amylase (3.2.1.1.)
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L2/00Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
    • A23L2/38Other non-alcoholic beverages
    • A23L2/382Other non-alcoholic beverages fermented
    • CCHEMISTRY; METALLURGY
    • C11ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11DDETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16Organic compounds
    • C11D3/38Products with no well-defined composition, e.g. natural products
    • C11D3/386Preparations containing enzymes, e.g. protease or amylase
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/24Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405Glucanases
    • C12N9/2408Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411Amylases
    • C12N9/2414Alpha-amylase (3.2.1.1.)
    • C12N9/2417Alpha-amylase (3.2.1.1.) from microbiological source
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/24Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405Glucanases
    • C12N9/2408Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411Amylases
    • C12N9/2414Alpha-amylase (3.2.1.1.)
    • C12N9/2417Alpha-amylase (3.2.1.1.) from microbiological source
    • C12N9/242Fungal source
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00Preparation of compounds containing saccharide radicals
    • C12P19/02Monosaccharides
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00Preparation of compounds containing saccharide radicals
    • C12P19/14Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase, e.g. by cellulase, hemicellulase
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y302/00Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01001Alpha-amylase (3.2.1.1)
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00Food compositions, function of food ingredients or processes for food or foodstuffs
    • C11D2111/12
    • C11D2111/14
DK13728877.5T 2012-06-08 2013-05-24 Variant-alfa-amylaser med forbedret aktivitet over for stivelsespolymerer DK2825643T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201261657501P 2012-06-08 2012-06-08
PCT/US2013/042702 WO2014007921A1 (en) 2012-06-08 2013-05-24 Variant alpha amylases with enhanced activity on starch polymers

Publications (2)

Publication Number Publication Date
DK2825643T1 DK2825643T1 (da) 2021-10-04
DK2825643T3 true DK2825643T3 (da) 2021-11-08

Family

ID=48626134

Family Applications (3)

Application Number Title Priority Date Filing Date
DK13728877.5T DK2825643T3 (da) 2012-06-08 2013-05-24 Variant-alfa-amylaser med forbedret aktivitet over for stivelsespolymerer
DK13729864.2T DK2859097T3 (da) 2012-06-08 2013-06-03 Alfa-amylase-varianter afledt fra alfa-amylasen af Cytophaga sp. amylase|(CspAmy2)
DKBA202000115U DK202000115Y3 (da) 2012-06-08 2020-11-04 Alfa-amylase-varianter afledt fra alfa-amylasen af Cytophaga sp. amylase (CspAmy2)

Family Applications After (2)

Application Number Title Priority Date Filing Date
DK13729864.2T DK2859097T3 (da) 2012-06-08 2013-06-03 Alfa-amylase-varianter afledt fra alfa-amylasen af Cytophaga sp. amylase|(CspAmy2)
DKBA202000115U DK202000115Y3 (da) 2012-06-08 2020-11-04 Alfa-amylase-varianter afledt fra alfa-amylasen af Cytophaga sp. amylase (CspAmy2)

Country Status (9)

Country Link
US (3) US20150141316A1 (da)
EP (4) EP4026902A1 (da)
CN (2) CN104379737B (da)
BR (1) BR112014030449A2 (da)
CA (2) CA2874061A1 (da)
DK (3) DK2825643T3 (da)
ES (2) ES2909509T3 (da)
HU (2) HUE057894T2 (da)
WO (2) WO2014007921A1 (da)

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DK2825643T1 (da) 2021-10-04
ES2909509T3 (es) 2022-05-06
DK202000115Y3 (da) 2021-04-09
DK2859097T1 (da) 2021-10-04
EP2859097B1 (en) 2021-11-03
CN104379737A (zh) 2015-02-25
EP2825643A1 (en) 2015-01-21
US20170037387A1 (en) 2017-02-09
EP2859097A1 (en) 2015-04-15
US20150141316A1 (en) 2015-05-21
DK202000115U1 (da) 2020-12-02
HUE057894T2 (hu) 2022-06-28
EP2859097B9 (en) 2023-03-08
CN104379738A (zh) 2015-02-25
US20150152401A1 (en) 2015-06-04
CA2874061A1 (en) 2014-01-09
HUE058058T2 (hu) 2022-07-28
CN104379737B (zh) 2018-10-23
ES2905323T3 (es) 2022-04-07
WO2014007921A1 (en) 2014-01-09
CA2874198A1 (en) 2013-12-12
WO2013184577A1 (en) 2013-12-12
BR112014030449A2 (pt) 2017-09-12
EP3967757B1 (en) 2024-01-31

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