DK2222842T3 - Glucoamylasevarianter med ændrede egenskaber - Google Patents

Glucoamylasevarianter med ændrede egenskaber Download PDF

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DK2222842T3
DK2222842T3 DK08851977.2T DK08851977T DK2222842T3 DK 2222842 T3 DK2222842 T3 DK 2222842T3 DK 08851977 T DK08851977 T DK 08851977T DK 2222842 T3 DK2222842 T3 DK 2222842T3
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DK08851977.2T
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Wolfgang Aehle
Richard R Bott
Martijin Scheffers
Solingen Piet Van
Casper Vroemen
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Danisco Us Inc
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C12N9/2428—Glucan 1,4-alpha-glucosidase (3.2.1.3), i.e. glucoamylase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/02—Monosaccharides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/14—Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase, e.g. by cellulase, hemicellulase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/06—Ethanol, i.e. non-beverage
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)

Claims (14)

  1. GLUCOAMYLASEVARIANTER MED ÆNDREDE EGENSKABER
    1. Glucoamylasevariant, der mindst omfatter: (i) en aminosyresubstitution ved position 61 i SEQ ID NO: 2; og (ii) en eller flere aminosyresubstitioner ved positioner svarende til positionerne 73, 417, 430, 431, 503, 511, 535, 539 eller 563 af SEQ ID NO: 2; hvor glucoamylasevarianten er mindst 80 % sekvensidentisk med SEQ ID NO: 1 eller 2, og hvor glucoamylasevarianten udviser øget termostabilitet eller forøget specifik aktivitet sammenlignet med glucoamylasen af SEQ ID NO: 2.
  2. 2. Glucoamylasevariant ifølge krav 1, hvor substitutionen ved position 61 i SEQ ID NO: 2 er udvalgt fra: N61D/I/L/Q/VAV.
  3. 3. Glucoamylasevariant ifølge krav 1 eller krav 2, hvor udgangsglucoamylasen har et katalytisk domæne, der er mindst 80 % sekvensidentisk med SEQ ID NO: 1 eller 2, eller et stivelsesbindingsdomæne, der er mindst 80 % sekvensidentisk med SEQ ID NO: 1 eller 2.
  4. 4. Glucoamylasevariant ifølge krav 1 eller krav 2, hvor glucoamylasevarianten er mindst 85 %, mindst 90 %, mindst 95 % eller mindst 99,5 % sekvensidentisk med SEQ ID NO: 1 eller 2, eller hvor udgangsglucoamylasen er SEQ ID NO: 1 eller 2.
  5. 5. Glucoamylasevariant ifølge krav 1 eller krav 2, hvor varianten omfatter aminosyresubstitioner svarende til N61I og en eller flere af G73F, L417R/V, T430A/M, A431L/Q, E503A/V, Q511H, A535R, A539R eller N563I/K i SEQ ID NO: 2.
  6. 6. Glucoamylasevariant ifølge krav 5, hvor varianten endvidere omfatter en eller flere af de følgende substitutioner: D4L/E/R/S/C/A/QAV, F5C/M/N/R/S/T/VAV, I12L/R, D24E/L/Y/T, F29L/1/D/C/S/V/W, I43F/R/D/Y/S/Q, D44E/H/K/S/N/Y/F/R/C, Y47W, Y49N, Q70R/K/M/P/G/L/F, Q75R/K/A, R76L/M/K/T/P, P94L, D100W/I/Q/M/P/A/N, N119P/T/Y/D/E, N146S/G/C/H/E/D/T/W/L/F/M, Q148V/Y/H/A/C/D/G/M/R/S/T, Y169D/F, Q172C/A/D/RÆ/F/H/V/L/M/N/S/T/V, F175H/A/G/R/S/T/CAV/Y, W178A/C/D/E/F/G/H/K/N/R/S/T/V/Y, E180A/C/G/H/I/L/N/P/Q/R/S/T/V/Y, VI81E/C/D/G/H/1/P/T/Y/S/L/K/F/A, Q208L/A/C/E/N/F/H/T, S211C/R/E/A/YAV/M/H/L/1/R/Q/T, E243 S/R/N/M/Y /A/L, R245A/E/M/I/P/V, I292D/H/P/R/T/N/V/F/L, G294C/D/E/T/Q/I/A, K297F/L/P/T/M/D/N/Q/A/Y/H/S/RAV, R309A/C/G/H/I/N/P/Q/S/TAV/Y/L, Y310E/G/L/P/S/W/R/Q, D313Q, V314A/R/N/D/C/E/Q/G/H/I/L/K/M/F/P/S/T/W/Y, Y315F, Y316Q/R, N317T/H, K340D/T, K341F/D/P/V/G/S, T350S/E/A/N, Q356H/D/E, T363L/R/C/HAV, S368W/D/F/L, S369F, N376Q/T/H/S/V, Y395Q/R/S, A398S/I/T, S401C/V, R408S, N409W/T/K, T412A/H/K/G, R433H/Q, 1436A/T eUer S451 M/T/H i SEQ ID NO: 2.
  7. 7. Glucoamylasevariant ifølge et hvilket som helst af de foregående krav, der omfatter et af substitutionssættene: I43Q/D44C/N611/L417 V/E503A/Q511H/A53 9R; I43Q/N61I/L417V/E503A/Q511H/A539R; I43Q/N61I/L417V/T430M/Q511H/A539R; I43R/N61I/L417R,V/E503A/Q511H/A539R; I43Q/N61I/T430A/A431L/Q511H/A539R; I43Q/N61I/T430A/Q51 IH; I43Q/N61I/T430A/Q511H/A539R; I43Q/N611/T430M/Q511H/A539R; I43Q/N61I/Q511H; I43Q/N61I/Q511H/A539R; i SEQ ID NO: 2.
  8. 8. Glucoamylasevariant ifølge et hvilket som helst af kravene 1 til 6, hvor udgangsglucoamylasen er udvalgt fra en glucoamylase, der er opnået fra en Trichoderma spp., en Aspergillus spp., en Humicola spp., en Penicillium spp., en Talaromycese spp. eller en Schizosaccharmyces spp.
  9. 9. Polynuklcotid, der koder for glucoamylasevarianten ifølge et hvilket som helst af kravene 1 til 8.
  10. 10. Vektor, der omfatter polynukleotidet ifølge krav 9.
  11. 11. Værtscelle, der omfatter vektoren ifølge krav 10.
  12. 12. Enzymsammensætning, der omfatter glucoamylasevarianten ifølge et hvilket som helst af kravene 1 til 8.
  13. 13. Fremgangsmåde til fremstilling af en glucoamylasevariant i en værtscelle, hvilken fremgangsmåde omfatter dyrkning af værtscellen ifølge krav 11 under forhold, der er egnede til ekspression og fremstilling af glucoamylasevarianten og fremstilling af glucoamylasevarianten.
  14. 14. Fremgangsmåde ifølge krav 13, der endvidere omfatter genvinding af glucoamylasevarianten fra dyrkningen.
DK08851977.2T 2007-11-20 2008-11-20 Glucoamylasevarianter med ændrede egenskaber DK2222842T3 (da)

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PCT/US2008/012934 WO2009067218A2 (en) 2007-11-20 2008-11-20 Glucoamylase variants with altered properties

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US (3) US8058033B2 (da)
EP (3) EP2222842B1 (da)
JP (1) JP5594899B2 (da)
CN (1) CN101868537B (da)
BR (1) BRPI0820483B1 (da)
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ES (2) ES2527586T3 (da)
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Families Citing this family (62)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9447397B2 (en) 2006-10-10 2016-09-20 Danisco Us Inc. Glucoamylase variants with altered properties
US8592194B2 (en) 2007-10-09 2013-11-26 Danisco Us Inc. Glucoamylase variants with altered properties
US20110039307A1 (en) * 2009-05-12 2011-02-17 Henderson Jodi M Ethanol yields in fermentation from an improved liquefaction process
CA2771071C (en) 2009-08-07 2020-03-10 Danisco Us Inc. Alpha-amylase blend for starch processing and method of use thereof
PE20121504A1 (es) * 2009-08-19 2012-11-05 Dupont Nutrition Biosci Aps Variantes de glucoamilasa
CN102712915B (zh) * 2009-08-19 2014-12-10 丹尼斯科美国公司 具有改良比活和/或热稳定性的葡糖淀粉酶的组合变体
DK2558484T3 (da) * 2010-04-14 2016-03-29 Novozymes As Polypeptider med glucoamylaseaktivitet og polynukleotider, der koder for dem
US9617527B2 (en) 2010-04-14 2017-04-11 Novozymes A/S Polypeptides having glucoamylase activity and polynucleotides encoding same
US20120034659A1 (en) 2010-08-06 2012-02-09 Danisco Us Inc. NEUTRAL pH SACCHARIFICATION AND FERMENTATION
US20120045812A1 (en) 2010-08-06 2012-02-23 Danisco Us Inc. PRODUCTION OF ISOPRENE UNDER NEUTRAL pH CONDITIONS
EP2702161A1 (en) 2011-04-29 2014-03-05 Danisco US Inc. Use of cellulase and glucoamylase to improve ethanol yields from fermentation
CN103732756A (zh) 2011-04-29 2014-04-16 丹尼斯科美国公司 用于高密度葡萄糖浆的淀粉液化和糖化的单pH工艺
US8951764B2 (en) 2011-08-05 2015-02-10 Danisco Us Inc. Production of isoprenoids under neutral pH conditions
AU2012328562A1 (en) 2011-10-28 2014-03-13 Danisco Us Inc. Variant maltohexaose-forming alpha-amylase variants
DK2788491T3 (da) 2011-12-09 2019-04-01 Danisco Us Inc Ribosomale promotorer fra b. subtilis til proteinproduktion i mikroorganismer
US20150152458A1 (en) 2012-03-28 2015-06-04 Danisco Us Inc. Low temperature method for making high glucose syrup
MX2014013402A (es) 2012-05-11 2014-11-26 Danisco Inc Uso de alfa-amilasa de aspergillus clavatus para sacarificacion.
WO2013181647A2 (en) 2012-06-01 2013-12-05 Danisco Us Inc. Compositions and methods of producing isoprene and/or industrrial bio-products using anaerobic microorganisms
DK2825643T3 (da) 2012-06-08 2021-11-08 Danisco Us Inc Variant-alfa-amylaser med forbedret aktivitet over for stivelsespolymerer
US20140024064A1 (en) 2012-07-23 2014-01-23 Butamax(Tm) Advanced Biofuels Llc Processes and systems for the production of fermentative alcohols
HUE034903T2 (hu) * 2012-08-14 2018-03-28 Danisco Us Inc Oxidációval kapcsolatos aktivitásvesztéssel szemben rezisztens trichoderma reesei glükoamiláz változatok és ezek felhasználása
JP2015534456A (ja) * 2012-08-16 2015-12-03 ダニスコ・ユーエス・インク アスペルギルス・クラバタス（Ａｓｐｅｒｇｉｌｌｕｓｃｌａｖａｔｕｓ）由来のαアミラーゼ及びイソアミラーゼを糖化に使用する方法
WO2014028434A2 (en) 2012-08-16 2014-02-20 Danisco Us Inc. Method of using alpha-amylase from aspergillus clavatus and pullulanase for saccharification
MX2015002099A (es) * 2012-08-22 2015-05-11 Dupont Nutrition Biosci Aps Variantes que tienen actividad glucoamilasa.
US9879241B2 (en) 2012-09-07 2018-01-30 Novozymes A/S Glucoamylase variants and polynucleotides encoding same and uses thereof
MX2015003073A (es) 2012-09-12 2015-07-14 Butamax Advanced Biofuels Llc Procesos y sistemas para la produccion de productos de fermentacion.
WO2014059273A1 (en) 2012-10-11 2014-04-17 Butamax Advanced Biofuels Llc Processes and systems for the production of fermentation products
WO2014081622A1 (en) 2012-11-20 2014-05-30 Danisco Us Inc. Amylase with maltogenic properties
CA2894513A1 (en) 2012-12-14 2014-06-19 Danisco Us Inc. Method of using alpha-amylase from aspergillus fumigatus and isoamylase for saccharification
US20160010128A1 (en) 2012-12-20 2016-01-14 Danisco Us Inc. Method of using alpha-amylase from aspergillus terreus and pullulanase for saccharification
WO2014099523A1 (en) 2012-12-21 2014-06-26 Danisco Us Inc. Alpha-amylase variants
WO2014099525A1 (en) 2012-12-21 2014-06-26 Danisco Us Inc. Paenibacillus curdlanolyticus amylase, and methods of use, thereof
US9695406B2 (en) * 2013-03-07 2017-07-04 Novozymes A/S Glucoamylase variants and polynucleotides encoding same
ES2676895T5 (es) 2013-03-11 2022-04-27 Danisco Us Inc Variantes combinatorias de alfa-amilasa
US9523104B2 (en) 2013-03-12 2016-12-20 Butamax Advanced Biofuels Llc Processes and systems for the production of alcohols
WO2014200658A1 (en) 2013-06-13 2014-12-18 Danisco Us Inc. Alpha-amylase from promicromonospora vindobonensis
WO2014200656A1 (en) 2013-06-13 2014-12-18 Danisco Us Inc. Alpha-amylase from streptomyces umbrinus
WO2014200657A1 (en) 2013-06-13 2014-12-18 Danisco Us Inc. Alpha-amylase from streptomyces xiamenensis
EP3011020A1 (en) 2013-06-17 2016-04-27 Danisco US Inc. Alpha-amylase from bacillaceae family member
CN105518130A (zh) 2013-06-21 2016-04-20 丹尼斯科美国公司 用于梭菌转化的组合物和方法
WO2015050723A1 (en) 2013-10-03 2015-04-09 Danisco Us Inc. Alpha-amylases from exiguobacterium, and methods of use, thereof
EP3052622B1 (en) 2013-10-03 2018-09-19 Danisco US Inc. Alpha-amylases from a subset of exiguobacterium, and methods of use, thereof
WO2015057517A1 (en) 2013-10-17 2015-04-23 Danisco Us Inc. Use of hemicellulases to improve ethanol production
BR112016009365A2 (pt) 2013-10-28 2017-09-19 Danisco Us Inc Método para aumentar, melhorar e estender a produção de etanol e método para diminuir as concentrações finais de dp2 em um produto de fermentação
WO2015066669A1 (en) 2013-11-04 2015-05-07 Danisco Us Inc. Proteases in corn processing
WO2015066667A1 (en) 2013-11-04 2015-05-07 Danisco Us Inc. Proteases in wheat processing
MX2016006489A (es) 2013-11-20 2016-08-03 Danisco Us Inc Alfa-amilasas variantes que tienen susceptibilidad reducida a la escision por proteasas y metodos de uso.
EP3077507B1 (en) 2013-12-04 2019-07-31 Danisco US Inc. Glucoamylase variants
WO2015094809A1 (en) 2013-12-19 2015-06-25 Danisco Us Inc. Chimeric fungal alpha-amylases comprising carbohydrate binding module and the use thereof
WO2015094714A1 (en) 2013-12-19 2015-06-25 Danisco Us Inc. Proteases in grain processing
CN103695386B (zh) * 2013-12-25 2015-09-16 南宁邦尔克生物技术有限责任公司 一种耐酸性高温β-淀粉酶突变体及其应用
CN107429267B (zh) * 2014-12-19 2021-07-16 丹尼斯科美国公司 葡糖淀粉酶共混物
DK3259358T3 (da) 2015-02-19 2024-09-09 Danisco Us Inc Forøget proteinekspression
EP3390625B1 (en) 2015-12-18 2023-09-06 Danisco US Inc. Polypeptides with endoglucanase activity and uses thereof
CN109071615A (zh) 2016-03-04 2018-12-21 丹尼斯科美国公司 用于在微生物中产生蛋白质的工程化核糖体启动子
US9598680B1 (en) * 2016-08-05 2017-03-21 Fornia Biosolutions, Inc. G16 glucoamylase compositions and methods
WO2019089898A1 (en) 2017-11-02 2019-05-09 Danisco Us Inc Freezing point depressed solid matrix compositions for melt granulation of enzymes
AU2020310162A1 (en) 2019-07-09 2022-02-03 International N&H Denmark Aps Fat coated particulate enzyme compositions
WO2021046073A1 (en) 2019-09-05 2021-03-11 Dupont Nutrition Biosciences Aps Feed composition
MX2024003691A (es) 2021-09-27 2024-06-26 Int N&H Denmark Aps Composiciones de aditivos de piensos y métodos para usar los mismos.
CA3242135A1 (en) 2021-12-24 2023-06-29 Zhongmei TANG Hybrid glucoamylases and methods of use thereof
WO2024137350A2 (en) 2022-12-19 2024-06-27 Danisco Us Inc. Recombinant fungal strains and methods thereof for producing consistent proteins

Family Cites Families (42)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5534046A (en) 1978-09-01 1980-03-10 Cpc International Inc Novel glucoamyrase having excellent heat resistance and production
GB2089836B (en) 1980-12-16 1984-06-20 Suntory Ltd Process for producing alcohol by fermentation without cooking
US4794175A (en) 1983-12-20 1988-12-27 Cetus Corporation Glucoamylase CDNA
US4618579A (en) 1984-09-28 1986-10-21 Genencor, Inc. Raw starch saccharification
JPH0630586B2 (ja) 1984-12-15 1994-04-27 サントリー株式会社 グルコアミラ−ゼ遺伝子
AU607398B2 (en) 1985-08-29 1991-03-07 Genencor Inc. Heterologous polypeptides expressed in filamentous fungi, processes for making same, and vectors for making same
US5024941A (en) 1985-12-18 1991-06-18 Biotechnica International, Inc. Expression and secretion vector for yeast containing a glucoamylase signal sequence
DK122686D0 (da) 1986-03-17 1986-03-17 Novo Industri As Fremstilling af proteiner
GB8610600D0 (en) 1986-04-30 1986-06-04 Novo Industri As Transformation of trichoderma
DE3854256T2 (de) 1987-06-06 1996-02-01 Omnigene Inc Expressionsvektor für hefe.
US6803499B1 (en) 1989-08-09 2004-10-12 Dekalb Genetics Corporation Methods and compositions for the production of stably transformed, fertile monocot plants and cells thereof
US6777589B1 (en) 1990-01-22 2004-08-17 Dekalb Genetics Corporation Methods and compositions for the production of stably transformed, fertile monocot plants and cells thereof
US5246853A (en) 1990-10-05 1993-09-21 Genencor International, Inc. Method for treating cotton-containing fabric with a cellulase composition containing endoglucanase components and which composition is free of exo-cellobiohydrolase I
US5290474A (en) 1990-10-05 1994-03-01 Genencor International, Inc. Detergent composition for treating cotton-containing fabrics containing a surfactant and a cellulase composition containing endolucanase III from trichoderma ssp
US5475101A (en) 1990-10-05 1995-12-12 Genencor International, Inc. DNA sequence encoding endoglucanase III cellulase
DK0551386T3 (da) 1990-10-05 2004-05-10 Genencor Int Fremgangsmåde til behandling af bomuldsholdige stoffer med cellulase
EP0562003B2 (en) 1990-12-10 2015-04-01 Danisco US Inc. Improved saccharification of cellulose by cloning and amplification of the beta-glucosidase gene of trichoderma reesei
US5861271A (en) 1993-12-17 1999-01-19 Fowler; Timothy Cellulase enzymes and systems for their expressions
ATE294871T1 (de) 1994-06-30 2005-05-15 Novozymes Biotech Inc Nicht-toxisches, nicht-toxigenes, nicht- pathogenes fusarium expressionssystem und darin zu verwendende promotoren und terminatoren
US5744716A (en) 1995-06-08 1998-04-28 Scp Global Technologies, A Division Of Preco, Inc. Fluid displacement level, density and concentration measurement system
US6537792B1 (en) 1996-07-24 2003-03-25 Iowa State University Protein engineering of glucoamylase to increase pH optimum, substrate specificity and thermostability
US6255084B1 (en) 1997-11-26 2001-07-03 Novozymes A/S Thermostable glucoamylase
US6352851B1 (en) 1998-07-15 2002-03-05 Novozymes A/S Glucoamylase variants
WO2000004136A1 (en) * 1998-07-15 2000-01-27 Novozymes A/S Glucoamylase variants
US6268328B1 (en) 1998-12-18 2001-07-31 Genencor International, Inc. Variant EGIII-like cellulase compositions
US6254914B1 (en) 1999-07-02 2001-07-03 The Board Of Trustees Of The University Of Illinois Process for recovery of corn coarse fiber (pericarp)
WO2001004273A2 (en) * 1999-07-09 2001-01-18 Novozymes A/S Glucoamylase variant
US20020182734A1 (en) 2000-08-11 2002-12-05 Diaz-Torres Maria R. Bacillus transformation, transformants and mutant libraries
US6582914B1 (en) 2000-10-26 2003-06-24 Genencor International, Inc. Method for generating a library of oligonucleotides comprising a controlled distribution of mutations
EP1434861A2 (en) * 2001-10-01 2004-07-07 Novozymes A/S Glucoamylase variants
GB0129864D0 (en) 2001-12-13 2002-02-06 Danisco Animal feed
CN1780560B (zh) 2003-03-10 2011-05-25 布罗因联合公司 利用生淀粉生产乙醇的方法
CN1788083B (zh) 2003-03-10 2011-10-05 诺维信公司 酒精产品生产方法
WO2005001036A2 (en) 2003-05-29 2005-01-06 Genencor International, Inc. Novel trichoderma genes
US6899910B2 (en) 2003-06-12 2005-05-31 The United States Of America As Represented By The Secretary Of Agriculture Processes for recovery of corn germ and optionally corn coarse fiber (pericarp)
CN1875099B (zh) 2003-11-21 2011-05-04 金克克国际有限公司 颗粒淀粉水解酶在木霉菌中的表达和从颗粒淀粉底物产生葡萄糖的方法
US7413887B2 (en) 2004-05-27 2008-08-19 Genecor International, Inc. Trichoderma reesei glucoamylase and homologs thereof
US7037704B2 (en) 2004-05-27 2006-05-02 Genencor International, Inc. Heterologous expression of an Aspergillus kawachi acid-stable alpha amylase and applications in granular starch hydrolysis
GB0423139D0 (en) 2004-10-18 2004-11-17 Danisco Enzymes
WO2006060062A2 (en) * 2004-11-30 2006-06-08 Genencor International, Inc. Trichoderma reesei glucoamylase and homologs thereof
JP5087407B2 (ja) 2004-12-30 2012-12-05 ジェネンコー・インターナショナル・インク 酸性真菌プロテアーゼ
US9447397B2 (en) * 2006-10-10 2016-09-20 Danisco Us Inc. Glucoamylase variants with altered properties

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