DK2788491T3 - Ribosomale promotorer fra b. subtilis til proteinproduktion i mikroorganismer - Google Patents
Ribosomale promotorer fra b. subtilis til proteinproduktion i mikroorganismer Download PDFInfo
- Publication number
- DK2788491T3 DK2788491T3 DK12806777.4T DK12806777T DK2788491T3 DK 2788491 T3 DK2788491 T3 DK 2788491T3 DK 12806777 T DK12806777 T DK 12806777T DK 2788491 T3 DK2788491 T3 DK 2788491T3
- Authority
- DK
- Denmark
- Prior art keywords
- promoter
- protein
- nucleic acid
- sequence
- expression
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
- C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
- C12N15/75—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
- C12Y304/21—Serine endopeptidases (3.4.21)
- C12Y304/21062—Subtilisin (3.4.21.62)
Landscapes
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Biomedical Technology (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Medicinal Chemistry (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Enzymes And Modification Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Claims (8)
1. Isoleret nukleinsyre, der omfatter en B. subtilis ribosomal RNA-promotor operativt bundet til en nukleinsyre, der koder for et protein af interesse, hvor proteinet af interesse er valgt fra gruppen bestående af en protease, cellulase, amylase, xylanase, phytase, mannanase, hemicellulase, carbohydrase, hydrolase, esterase, oxidase, permease, pullulanase, laccase, lipase, reductase, isomerase, epimerase, tautomerase, transferase, kinase eller phosphatase, hvor den ribosomale RNA-promotor omfatter nukleotidsekvensen ifølge et hvilket som helst af SEQ ID NO: 2 og 3, en nukleinsyre, der er en undersekvens med mindst 80 % sekvensidentitet med et hvilket som helst af SEQ ID NO: 2 og 3, der bevarer promotoraktivitet, eller kombinationer deraf.
2. Isoleret nukleinsyre ifølge krav 1, hvor nukleinsyren omfatter nukleotidsekvensen ifølge SEQ ID NO: 3.
3. Isoleret nukleinsyre ifølge et hvilket som helst af de foregående krav, hvor proteinet af interesse er en protease.
4. Isoleret nukleinsyre ifølge krav 3, hvor proteasen er subtilisin.
5. Isoleret nukleinsyre ifølge krav 3, hvor proteasen er kodet for af SEQ ID NO: 9, 11, 18 eller 20.
6. Ekspressionsvektor, der omfatter nukleinsyren ifølge et hvilket som helst af de foregående krav.
7. Mikroorganisme, der omfatter ekspressionsvektoren ifølge krav 6.
8. Fremgangsmåde til fremstilling af et protein af interesse, der omfatter dyrkning af mikroorganismen ifølge krav 7 under forhold, der er egnede til, at mikroorganismen producerer proteinet, og eventuelt til genvinding af det således producerede protein af interesse.
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US201161569202P | 2011-12-09 | 2011-12-09 | |
US201161577491P | 2011-12-19 | 2011-12-19 | |
PCT/US2012/068285 WO2013086219A1 (en) | 2011-12-09 | 2012-12-06 | Ribosomal promotors from b. subtilis for protein production in microorganisms |
Publications (1)
Publication Number | Publication Date |
---|---|
DK2788491T3 true DK2788491T3 (da) | 2019-04-01 |
Family
ID=47436212
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
DK12806777.4T DK2788491T3 (da) | 2011-12-09 | 2012-12-06 | Ribosomale promotorer fra b. subtilis til proteinproduktion i mikroorganismer |
Country Status (6)
Country | Link |
---|---|
US (1) | US20140329309A1 (da) |
EP (1) | EP2788491B1 (da) |
JP (1) | JP6378089B2 (da) |
CN (1) | CN104053780A (da) |
DK (1) | DK2788491T3 (da) |
WO (1) | WO2013086219A1 (da) |
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KR20170116159A (ko) * | 2015-02-19 | 2017-10-18 | 다니스코 유에스 인크. | 향상된 단백질 발현 |
WO2017075195A1 (en) | 2015-10-30 | 2017-05-04 | Danisco Us Inc | Enhanced protein expression and methods thereof |
KR20180086206A (ko) * | 2015-12-14 | 2018-07-30 | 바스프 에스이 | 바실루스로부터의 변형된 양방향성 카탈라제 프로모터 |
EP3380496B1 (en) | 2015-12-23 | 2020-05-20 | Danisco US Inc. | Enhanced protein production and methods thereof |
US11447782B2 (en) | 2016-03-04 | 2022-09-20 | Danisco Us Inc. | Engineered ribosomal promoters for protein production in microorganisms |
EP4151726A1 (en) | 2016-06-17 | 2023-03-22 | Danisco US Inc | Protease variants and uses thereof |
RU2771261C2 (ru) | 2016-12-21 | 2022-04-29 | ДюПон НЬЮТРИШН БАЙОСАЙЕНСИЗ АпС | Способы применения термостабильных сериновых протеаз |
WO2018118917A1 (en) | 2016-12-21 | 2018-06-28 | Danisco Us Inc. | Protease variants and uses thereof |
US11946081B2 (en) | 2016-12-21 | 2024-04-02 | Danisco Us Inc. | Bacillus gibsonii-clade serine proteases |
EP3571217B1 (en) | 2017-01-23 | 2023-06-21 | Danisco US Inc. | Methods and compositions for obtaining natural competence in bacillus host cells |
EP4353828A2 (en) | 2017-02-24 | 2024-04-17 | Danisco US Inc. | Compositions and methods for increased protein production in bacillus licheniformis |
EP3583210B1 (en) | 2017-03-15 | 2021-07-07 | Danisco US Inc. | Trypsin-like serine proteases and uses thereof |
RU2019132448A (ru) | 2017-03-15 | 2021-04-15 | ДюПон НЬЮТРИШН БАЙОСАЙЕНСИЗ АпС | Способы применения сериновой протеазы архей |
US20200015499A1 (en) | 2017-03-15 | 2020-01-16 | Dupont Nutrition Biosciences Aps | Trypsin-like serine proteases and uses thereof |
KR102533803B1 (ko) | 2017-04-07 | 2023-05-18 | 듀폰 뉴트리션 바이오사이언시즈 에이피에스 | p-니트로벤질에스터라제 부활성의 부재 하에 β-갈락토시다제 및 락타제를 생산하는 바실러스 숙주 세포 |
WO2019055261A1 (en) | 2017-09-13 | 2019-03-21 | Danisco Us Inc | MODIFIED 5 'NON-TRANSLATED REGION (UTR) SEQUENCES FOR INCREASED PROTEIN PRODUCTION IN BACILLUS |
EP3717643A1 (en) | 2017-11-29 | 2020-10-07 | Danisco US Inc. | Subtilisin variants having improved stability |
US11414643B2 (en) | 2018-01-03 | 2022-08-16 | Danisco Us Inc. | Mutant and genetically modified Bacillus cells and methods thereof for increased protein production |
CN110004166A (zh) * | 2018-01-05 | 2019-07-12 | 中国科学院天津工业生物技术研究所 | 高效表达分泌β-甘露聚糖酶的重组枯草芽孢杆菌菌株及其获得方法 |
CN108441489B (zh) * | 2018-04-09 | 2023-04-14 | 潍坊康地恩生物科技有限公司 | 一种蛋白生产方法及高产碱性蛋白酶的枯草芽孢杆菌 |
WO2019245705A1 (en) | 2018-06-19 | 2019-12-26 | Danisco Us Inc | Subtilisin variants |
WO2019245704A1 (en) | 2018-06-19 | 2019-12-26 | Danisco Us Inc | Subtilisin variants |
CN112654703A (zh) | 2018-07-06 | 2021-04-13 | 杜邦营养生物科学有限公司 | 用于基于谷类的动物饲料的含木聚糖酶饲料添加剂 |
US11781147B2 (en) * | 2018-11-28 | 2023-10-10 | Danisco Us Inc. | Promoter sequences and methods thereof for enhanced protein production in Bacillus cells |
EP3947656A1 (en) | 2019-04-05 | 2022-02-09 | Danisco US Inc. | Methods for polynucleotide integration into the genome of bacillus using dual circular recombinant dna constructs and compositions thereof |
KR20210148269A (ko) | 2019-04-05 | 2021-12-07 | 다니스코 유에스 인크. | 선형 재조합 dna 작제물 및 이의 조성물을 이용하여 공여 dna 서열을 바실러스 게놈 내에 통합시키기 위한 방법 |
WO2021086606A1 (en) | 2019-10-28 | 2021-05-06 | Danisco Us Inc | Microbial host cells for the production of heterologous cyanuric acid hydrolases and biuret hydrolases |
EP4061939A1 (en) | 2019-11-19 | 2022-09-28 | Danisco US Inc. | Selection marker free methods for modifying the genome of bacillus and compositions thereof |
AU2020417807A1 (en) * | 2019-12-31 | 2022-07-07 | Air Protein, Inc. | High protein food compositions |
JP2023524334A (ja) | 2020-01-15 | 2023-06-12 | ダニスコ・ユーエス・インク | バチルス・リケニフォルミス(bacillus licheniformis)における強化したタンパク質産生のための組成物及び方法 |
WO2023225459A2 (en) | 2022-05-14 | 2023-11-23 | Novozymes A/S | Compositions and methods for preventing, treating, supressing and/or eliminating phytopathogenic infestations and infections |
WO2024050503A1 (en) * | 2022-09-02 | 2024-03-07 | Danisco Us Inc. | Novel promoter and 5'-untranslated region mutations enhancing protein production in gram-positive cells |
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-
2012
- 2012-12-06 DK DK12806777.4T patent/DK2788491T3/da active
- 2012-12-06 CN CN201280059922.9A patent/CN104053780A/zh active Pending
- 2012-12-06 US US14/364,016 patent/US20140329309A1/en not_active Abandoned
- 2012-12-06 WO PCT/US2012/068285 patent/WO2013086219A1/en unknown
- 2012-12-06 EP EP12806777.4A patent/EP2788491B1/en active Active
- 2012-12-06 JP JP2014546087A patent/JP6378089B2/ja active Active
Also Published As
Publication number | Publication date |
---|---|
WO2013086219A1 (en) | 2013-06-13 |
CN104053780A (zh) | 2014-09-17 |
EP2788491B1 (en) | 2019-01-23 |
JP6378089B2 (ja) | 2018-08-22 |
JP2015500032A (ja) | 2015-01-05 |
EP2788491A1 (en) | 2014-10-15 |
US20140329309A1 (en) | 2014-11-06 |
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