KR950702240A - 세제로의 이용을 위한 새로운 리파제 변형체 - Google Patents

세제로의 이용을 위한 새로운 리파제 변형체

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Publication number
KR950702240A
KR950702240A KR1019940704762A KR19940704762A KR950702240A KR 950702240 A KR950702240 A KR 950702240A KR 1019940704762 A KR1019940704762 A KR 1019940704762A KR 19940704762 A KR19940704762 A KR 19940704762A KR 950702240 A KR950702240 A KR 950702240A
Authority
KR
South Korea
Prior art keywords
lipase
modified
recombinant dna
modified lipase
pseudomonas
Prior art date
Application number
KR1019940704762A
Other languages
English (en)
Inventor
메츠케 반데르란 잔
버나두스 마리아 렌팅 헤르마누스
조하네스 소피에 마리에 물레너스 레오나르두스
미틸드 조세피나 콕스 마리아
Original Assignee
한스 발터 라벤
기스트-브로카데스 나암로제 베노오트하프
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
Application filed by 한스 발터 라벤, 기스트-브로카데스 나암로제 베노오트하프 filed Critical 한스 발터 라벤
Publication of KR950702240A publication Critical patent/KR950702240A/ko

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18—Carboxylic ester hydrolases (3.1.1)
    • C12N9/20—Triglyceride splitting, e.g. by means of lipase
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16—Organic compounds
    • C11D3/38—Products with no well-defined composition, e.g. natural products
    • C11D3/386—Preparations containing enzymes, e.g. protease or amylase
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16—Organic compounds
    • C11D3/38—Products with no well-defined composition, e.g. natural products
    • C11D3/386—Preparations containing enzymes, e.g. protease or amylase
    • C11D3/38627—Preparations containing enzymes, e.g. protease or amylase containing lipase

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Detergent Compositions (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

야생형 Pseudomones pseudoalcaligenes 리파제안의 위치 21에 대응하는 위치에서 적어도 메티오닌이 다른 아미노산에 의해서 치환되는 변형된 리파제가 개시되어 있고, 상기 리파제는 소망하는 성질변화, 특히 개선된 세척성능을 나타내고 있다.
바람직한 구체예는 류신, 세린 또는 알라닝에 의한 상기 메티오닌의 치환이다.

Description

세제로의 이용을 위한 새로운 리파제 변형체
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음
제1도는 PCR을 이용한 부위 특이적 돌연변이 유발,
제2도는 염색체내로 플라스미드 pBR 플랭크(flank)의 삽입,
제3도는 염색체로부터 리파제 유전자 및 플라스미드 결실에 대한 재조합,
제4도는 염색체 및 플라스미드내로 돌연변이 리파제 DNA의도입.
제5도는 3' 측면부위에서의 재조합.

Claims (13)

  1. 야생형 Pseudomomas pseudoalcaligenes 리파제안의 위치 21에 대응하는 위치에서 적어도 메티오닌이 다른 아미노산에 의해서 치환되는 변형된 리파제에 있어서, 상기 리파제는 소망하는 성질변화를 나타내고 있는 것을 특징으로 하는 변형된 리파제.
  2. 제1항에 있어서, 야생형 리파제는 Pseudomonas I군 미생물로 부터 생기는 것을 특징으로 하는 변형된 리파제.
  3. 제1항 또는 제2항에 있어서, 야생형 리파제는 Pseudomonas pseudoalcaligenes 리파제와의 적어도 70% 상동성을 가지는 것을 특징으로 하는 변형된 리파제.
  4. 제1항 내지 제3항중의 어느 한항에 있어서, 위치 21의 메티오닌이 류신, 세린 또는 알라닌에 의해서 치환되는 것을 특징으로 하는 변형된 리파제.
  5. 제1항 내지 제4항중의 어느 한항에 있어서, 개선된 세척성능을 나타내고 있는 것을 특징으로 하는 변형된 리파제.
  6. 제1항 내지 제5항중의 어느 한항에 따른 변형된 리파제로 이루어진 세제조성물.
  7. 세제조성물로서 제1항 내지 제6항중의 어느 한항에 따른 변형된 리파제의 이용.
  8. 제1항 내지 제5항중의 어느 한항에 따른 변형된 리파제를 암호하는 재조합 DNA서열.
  9. 제8항의 재조합 DNA서열로 이루어지고, 변형된 리파제의 발현에 유용한 것을 특징으로 하는 재조합 DNA 백터.
  10. 제8항에 따른 재조합 DNA서열 또는 제9항에 따른 재조합 DNA벡터로 이루어진 것을 특징으로 하는 형질전환 숙주 미생물.
  11. 제10항에 있어서, Pseudomonas 인 것을 특징으로 하는 형질전환 숙주 미생물.
  12. Pseudomonas pseudoalcaligenes 리파제안의 Met+21에 대응하는 적어도 하나의 위치에서 pseudoalcaligenas리파제를 암호화하는 DNA에서 돌연변이를 유발시키고, 상기 돌연변이로부터 초래되는 효소에서의 소망하는 성질변화에 대해서 시험하는 것으로 이루어진 방법.
  13. 제12항에 있어서, 상기 성질변화가 개선된 세척성능, 변화된 특이적 활성, 변화된 pH활성, 변화된 기질 활성 및 강화된 산화안정성중의 하나이상으로부터 선택되는 것을 특징으로 하는 방법.
    ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
KR1019940704762A 1993-04-27 1994-04-27 세제로의 이용을 위한 새로운 리파제 변형체 KR950702240A (ko)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP93201212 1993-04-27
EP93201212.3 1993-04-27
PCT/EP1994/001435 WO1994025578A1 (en) 1993-04-27 1994-04-27 New lipase variants for use in detergent applications

Publications (1)

Publication Number Publication Date
KR950702240A true KR950702240A (ko) 1995-06-19

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Country Status (12)

Country Link
EP (1) EP0652946B1 (ko)
JP (1) JP3618748B2 (ko)
KR (1) KR950702240A (ko)
CN (1) CN1108457A (ko)
AT (1) ATE287946T1 (ko)
AU (1) AU673078B2 (ko)
CA (1) CA2138519C (ko)
DE (1) DE69434242T2 (ko)
DK (1) DK0652946T3 (ko)
FI (1) FI116728B (ko)
PL (1) PL306812A1 (ko)
WO (1) WO1994025578A1 (ko)

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