DE69129988T2 - Lipase-varianten - Google Patents

Lipase-varianten

Info

Publication number
DE69129988T2
DE69129988T2 DE69129988T DE69129988T DE69129988T2 DE 69129988 T2 DE69129988 T2 DE 69129988T2 DE 69129988 T DE69129988 T DE 69129988T DE 69129988 T DE69129988 T DE 69129988T DE 69129988 T2 DE69129988 T2 DE 69129988T2
Authority
DE
Germany
Prior art keywords
contact zone
lipid contact
lipases
change
lipid
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69129988T
Other languages
English (en)
Other versions
DE69129988D1 (de
Inventor
Allan Svendsen
Ib Clausen
Shamkant Patkar
Erik Gormsen
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Novozymes AS
Original Assignee
Novo Nordisk AS
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from DK219490A external-priority patent/DK219490D0/da
Priority claimed from DK219590A external-priority patent/DK219590D0/da
Priority claimed from DK219690A external-priority patent/DK219690D0/da
Application filed by Novo Nordisk AS filed Critical Novo Nordisk AS
Publication of DE69129988D1 publication Critical patent/DE69129988D1/de
Application granted granted Critical
Publication of DE69129988T2 publication Critical patent/DE69129988T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16—Organic compounds
    • C11D3/38—Products with no well-defined composition, e.g. natural products
    • C11D3/386—Preparations containing enzymes, e.g. protease or amylase
    • C11D3/38627—Preparations containing enzymes, e.g. protease or amylase containing lipase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C12N15/625—DNA sequences coding for fusion proteins containing a sequence coding for a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18—Carboxylic ester hydrolases (3.1.1)
    • C12N9/20—Triglyceride splitting, e.g. by means of lipase
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/04—Fusion polypeptide containing a localisation/targetting motif containing an ER retention signal such as a C-terminal HDEL motif
DE69129988T 1990-09-13 1991-09-13 Lipase-varianten Expired - Lifetime DE69129988T2 (de)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
DK219490A DK219490D0 (da) 1990-09-13 1990-09-13 Enzymmutanter
DK219590A DK219590D0 (da) 1990-09-13 1990-09-13 Lipasemutanter
DK219690A DK219690D0 (da) 1990-09-13 1990-09-13 Lipasevarianter
PCT/DK1991/000271 WO1992005249A1 (en) 1990-09-13 1991-09-13 Lipase variants

Publications (2)

Publication Number Publication Date
DE69129988D1 DE69129988D1 (de) 1998-09-17
DE69129988T2 true DE69129988T2 (de) 1999-03-18

Family

ID=27221523

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69129988T Expired - Lifetime DE69129988T2 (de) 1990-09-13 1991-09-13 Lipase-varianten

Country Status (12)

Country Link
EP (1) EP0548228B1 (de)
JP (1) JP3469234B2 (de)
KR (1) KR930702514A (de)
AT (1) ATE169671T1 (de)
AU (1) AU657278B2 (de)
BR (1) BR9106839A (de)
CA (1) CA2092615A1 (de)
DE (1) DE69129988T2 (de)
DK (1) DK0548228T3 (de)
ES (1) ES2121786T3 (de)
FI (1) FI120044B (de)
WO (1) WO1992005249A1 (de)

Cited By (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7666618B2 (en) 2004-07-16 2010-02-23 Danisco A/S Lipolytic enzyme: uses thereof in the food industry
US7718408B2 (en) 2003-12-24 2010-05-18 Danisco A/S Method
US7718204B2 (en) 1998-07-21 2010-05-18 Danisco A/S Foodstuff
US7807398B2 (en) 2003-01-17 2010-10-05 Danisco A/S Method of using lipid acyltransferase
US7955814B2 (en) 2003-01-17 2011-06-07 Danisco A/S Method
US7960150B2 (en) 2007-01-25 2011-06-14 Danisco A/S Production of a lipid acyltransferase from transformed Bacillus licheniformis cells
US8012732B2 (en) 2004-03-12 2011-09-06 Danisco A/S Fungal lypolytic and amylase enzyme composition and methods using the same
US8030044B2 (en) 2003-12-24 2011-10-04 Danisco A/S Lipid acyltransferases
USRE43135E1 (en) 2001-05-18 2012-01-24 Danisco A/S Method of improving dough and bread quality
USRE43341E1 (en) 1995-06-07 2012-05-01 Danisco A/S Method of improving the properties of a flour dough, a flour dough improving composition and improved food products
US8652809B2 (en) 2007-08-17 2014-02-18 Dupont Nutrition Biosciences Aps Method for producing ultra-heat treatment milk

Families Citing this family (646)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5892013A (en) * 1990-09-13 1999-04-06 Novo Nordisk A/S Lipase variants
DK46693D0 (de) * 1993-04-23 1993-04-23 Novo Nordisk As
WO1993000426A1 (en) * 1991-06-25 1993-01-07 Novo Nordisk A/S Mammalian pancreatic lipase and variant thereof
DK88892D0 (da) * 1992-07-06 1992-07-06 Novo Nordisk As Forbindelse
SK79595A3 (en) * 1992-12-18 1995-11-08 Unilever Nv Method of production of modified cutinases, dna vector and host
CZ163995A3 (en) * 1992-12-23 1995-11-15 Unilever Nv VARIANTS OF CUTINASES WITH ADJUSTED COMPATIBILITY TO ANIONIC WETTING AGENTS, PRODUCTION PROCESS FOR SAID CUTINASE VARIANTS, BY MICRO-ORGANISMS TREATED rDNA, POLYNUCLEOTIDES-CONTAINING SEQUENCE OF NUCLEOTIDES ENCODING THE CUTINASE VARIANTS, VECTORS OF RECOMBINANT DNA CAPABLE OF CONTROL GENE EXPRESSION FOR SAID CUTINASE VARIANTS, AND ENZYMATIC DETERGENTS CONTAINING THE CUTINASE VARIANTS
JPH09503130A (ja) * 1993-10-04 1997-03-31 ノボ ノルディスク アクティーゼルスカブ 修飾酵素を含んで成る酵素調製品
DE69527835T2 (de) * 1994-02-22 2003-04-10 Novozymes As Methode zur herstellung einer variante eines lipolytischen enzymes
AU2884595A (en) * 1994-06-20 1996-01-15 Unilever Plc Modified pseudomonas lipases and their use
FR2722798B1 (fr) * 1994-07-25 1996-09-13 Toulouse Inst Nat Polytech 1procede de production d'acides gras2plantes oleagineuses
WO1997000929A1 (en) * 1994-10-13 1997-01-09 The Procter & Gamble Company Detergent compositions containing amines and anionic surfactants
US5935271A (en) * 1994-10-13 1999-08-10 Procter & Gamble Company Laundry detergent compositions containing lipolytic enzyme and amines
CN1172498A (zh) * 1994-11-18 1998-02-04 普罗格特-甘布尔公司 含有脂肪酶和蛋白酶的洗涤剂组合物
EP0792342B1 (de) * 1994-11-18 2001-09-05 The Procter & Gamble Company Verwendung von spezifische lipolytische enzyme in reinigungsmitteln
GB2297979A (en) * 1995-02-15 1996-08-21 Procter & Gamble Detergent compositions containing a lipase enzyme
SK125697A3 (en) * 1995-03-23 1998-05-06 Astra Ab A dna molecule for expression of bile salt-stimulated lipase (bssl)
JP4307549B2 (ja) * 1995-07-14 2009-08-05 ノボザイムス アクティーゼルスカブ 脂肪分解活性を有する修飾された酵素
US6495357B1 (en) 1995-07-14 2002-12-17 Novozyme A/S Lipolytic enzymes
JP4068142B2 (ja) * 1995-08-11 2008-03-26 ノボザイムス アクティーゼルスカブ 新規の脂肪分解酵素
DE69628026T2 (de) * 1995-09-07 2004-04-08 Novozymes A/S Nachweis der aktivität von enzymen für detergenzien durch phagen.
EP0873387A1 (de) * 1995-09-29 1998-10-28 The Procter & Gamble Company Flüssige waschmittelenthaltend ausgewählte ammoniumverbindungen
AU7528196A (en) * 1995-11-17 1997-06-11 Procter & Gamble Company, The Laundry detergent compositions containing lipolytic enzyme and selected quaternary ammonium compounds
WO1997020918A1 (en) * 1995-12-07 1997-06-12 Recombinant Biocatalysis, Inc. Method of screening for enzyme activity
CA2254781A1 (en) * 1996-05-15 1997-11-20 The Procter & Gamble Company Detergent compositions comprising specific lipolytic enzyme and a specific surfactant system
WO1997043374A1 (en) * 1996-05-15 1997-11-20 The Procter & Gamble Company Detergent compositions comprising specific lipolytic enzyme and a soil release polymer
WO1997043380A1 (en) * 1996-05-15 1997-11-20 The Procter & Gamble Company Detergent compositions comprising specific lipolytic enzyme and zeolite map
EP0912683A1 (de) * 1996-05-15 1999-05-06 The Procter & Gamble Company Waschmittelzusammensetzungen enthaltend ein spezifisches lipolytisches enzym und alkylpolyglykosidtensid
JPH11511778A (ja) * 1996-05-15 1999-10-12 ザ、プロクター、エンド、ギャンブル、カンパニー 脂肪分解酵素を含んでなる洗剤組成物
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US6156552A (en) * 1998-02-18 2000-12-05 Novo Nordisk A/S Lipase variants
ATE490312T1 (de) 1998-02-27 2010-12-15 Novozymes As Maltogene alpha-amylase varianten
AU6188599A (en) 1998-10-26 2000-05-15 Novozymes A/S Constructing and screening a dna library of interest in filamentous fungal cells
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US7312062B2 (en) 1998-11-27 2007-12-25 Novozymes A/S Lipolytic enzyme variants
ATE504651T1 (de) 1998-12-18 2011-04-15 Novozymes As Subtilase enzyme der i-s1- und i-s2-untergruppen mit einem zusätzlichen aminosäurerest in einer aktiven schleifenregion
CN100532561C (zh) 1999-03-22 2009-08-26 诺沃奇梅兹有限公司 新的葡糖淀粉酶及编码它的核酸序列
AU3419200A (en) 1999-03-31 2000-10-23 Novozymes A/S Polypeptides having alkaline alpha-amylase activity and nucleic acids encoding same
CA2366843A1 (en) 1999-03-31 2000-10-12 Jesper Vind Lipase variant
WO2000060063A1 (en) 1999-03-31 2000-10-12 Novozymes A/S Lipase variant
DK2011864T3 (en) 1999-03-31 2015-04-07 Novozymes As Polypeptides with alkaline alpha-amylase activity and nucleic acids encoding them
WO2000071688A1 (en) 1999-05-20 2000-11-30 Novozymes A/S Subtilase enzymes of the i-s1 and i-s2 sub-groups having at least one additional amino acid residue between positions 126 and 127
WO2000071690A1 (en) 1999-05-20 2000-11-30 Novozymes A/S Subtilase enzymes of the i-s1 and i-s2 sub-groups having at least one additional amino acid residue between positions 128 and 129
ATE408676T1 (de) 1999-05-20 2008-10-15 Novozymes As Subtilase enzyme der i-s1 und i-s2 untergruppen mit mindestens einer zusätzlichen aminosäure zwischen position 132 und 133
EP1183343B2 (de) 1999-05-20 2013-11-27 Novozymes A/S Subtilase-enzyme der i-s1 und i-s2 untergruppen mit wenigstens einem zusätzlichen aminosäurerest zwischen positionen 125 und 126
ATE408679T1 (de) 1999-05-20 2008-10-15 Novozymes As Subtilase enzyme der i-s1 und i-s2 untergruppen mit mindestens einer zusätzlichen aminosäure zwischen position 127 und 128
DE60040284D1 (de) 1999-05-20 2008-10-30 Novozymes As Subtilase enzyme der i-s1 und i-s2 untergruppen mit mindestens einer zusätzlichen aminosäure zwischen position 129 und 130
WO2000075295A1 (en) * 1999-06-02 2000-12-14 Novozymes A/S Chemically modified lipolytic enzyme
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US7972638B2 (en) 1998-07-21 2011-07-05 Danisco A/S Foodstuff
US8163315B2 (en) 1998-07-21 2012-04-24 Danisco A/S Foodstuff
US7718204B2 (en) 1998-07-21 2010-05-18 Danisco A/S Foodstuff
US7781001B2 (en) 1998-07-21 2010-08-24 Danisco A/S Foodstuff
USRE43135E1 (en) 2001-05-18 2012-01-24 Danisco A/S Method of improving dough and bread quality
US8278062B2 (en) 2003-01-14 2012-10-02 Dupont Nutrition Biosciences Aps Method of using lipid acyltransferase
US7955813B2 (en) 2003-01-17 2011-06-07 Danisco, A/S Method of using lipid acyltransferase
US8003095B2 (en) 2003-01-17 2011-08-23 Danisco A/S Method of using lipid acyltransferase
US7955814B2 (en) 2003-01-17 2011-06-07 Danisco A/S Method
US7807398B2 (en) 2003-01-17 2010-10-05 Danisco A/S Method of using lipid acyltransferase
US8440435B2 (en) 2003-12-24 2013-05-14 Dupont Nutrition Biosciences Aps Method for reducing 1,2-diglyceride content of an edible oil
US8030044B2 (en) 2003-12-24 2011-10-04 Danisco A/S Lipid acyltransferases
US7718408B2 (en) 2003-12-24 2010-05-18 Danisco A/S Method
US8012732B2 (en) 2004-03-12 2011-09-06 Danisco A/S Fungal lypolytic and amylase enzyme composition and methods using the same
US8192782B2 (en) 2004-07-16 2012-06-05 Danisco A/S Enzymatic oil-degumming method
US7666618B2 (en) 2004-07-16 2010-02-23 Danisco A/S Lipolytic enzyme: uses thereof in the food industry
US8535900B2 (en) 2004-07-16 2013-09-17 Dupont Nutrition Biosciences Aps Lipolytic enzyme uses thereof in the food industry
US8889371B2 (en) 2004-07-16 2014-11-18 Dupont Nutrition Biosciences Aps Lipolytic enzyme: uses thereof in the food industry
US7960150B2 (en) 2007-01-25 2011-06-14 Danisco A/S Production of a lipid acyltransferase from transformed Bacillus licheniformis cells
US8652809B2 (en) 2007-08-17 2014-02-18 Dupont Nutrition Biosciences Aps Method for producing ultra-heat treatment milk

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ATE169671T1 (de) 1998-08-15
AU8617291A (en) 1992-04-15
FI931124A0 (fi) 1993-03-12
WO1992005249A1 (en) 1992-04-02
JPH06501153A (ja) 1994-02-10
BR9106839A (pt) 1993-07-20
FI120044B (fi) 2009-06-15
DK0548228T3 (da) 1999-05-10
DE69129988D1 (de) 1998-09-17
JP3469234B2 (ja) 2003-11-25
KR930702514A (ko) 1993-09-09
FI931124A (fi) 1993-05-12
AU657278B2 (en) 1995-03-09
CA2092615A1 (en) 1992-03-14
EP0548228B1 (de) 1998-08-12
ES2121786T3 (es) 1998-12-16
EP0548228A1 (de) 1993-06-30

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