JPS58117455A - 抗体を検出及び定量する方法 - Google Patents

抗体を検出及び定量する方法

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Publication number
JPS58117455A
JPS58117455A JP57193265A JP19326582A JPS58117455A JP S58117455 A JPS58117455 A JP S58117455A JP 57193265 A JP57193265 A JP 57193265A JP 19326582 A JP19326582 A JP 19326582A JP S58117455 A JPS58117455 A JP S58117455A
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JP
Japan
Prior art keywords
antibodies
antibody
enzyme
protein
amount
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アントニウス・ヘルマヌス・ウイルヘルムス・マリア・シユ−ルズ
バウケ・クラ−ス・バン・ウイ−メン
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ORUGANON NV
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ORUGANON NV
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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/74—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving hormones or other non-cytokine intercellular protein regulatory factors such as growth factors, including receptors to hormones and growth factors
    • G01N33/743—Steroid hormones
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
    • G01N33/537—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with separation of immune complex from unbound antigen or antibody
    • G01N33/539—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with separation of immune complex from unbound antigen or antibody involving precipitating reagent, e.g. ammonium sulfate
    • G01N33/541—Double or second antibody, i.e. precipitating antibody
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/964—Chemistry: molecular biology and microbiology including enzyme-ligand conjugate production, e.g. reducing rate of nonproductive linkage
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/971—Capture of complex after antigen-antibody reaction
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/975—Kit
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S436/00—Chemistry: analytical and immunological testing
    • Y10S436/807—Apparatus included in process claim, e.g. physical support structures
    • Y10S436/808—Automated or kit
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S436/00—Chemistry: analytical and immunological testing
    • Y10S436/815—Test for named compound or class of compounds
    • Y10S436/817—Steroids or hormones
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S436/00—Chemistry: analytical and immunological testing
    • Y10S436/815—Test for named compound or class of compounds
    • Y10S436/817—Steroids or hormones
    • Y10S436/818—Human chorionic gonadotropin

Abstract

(57)【要約】本公報は電子出願前の出願データであるた
め要約のデータは記録されません。

Description

【発明の詳細な説明】 特電の結合活性を持つ蛋白質（ａ　ｓｐ＠ｅｌｆｌａｂ
ｌｎｄｉｎｇ　ｐｒｏｔｅｉｎ　）とそれに対応して結
合する物質（ｔｈｅ　ｃｏｒｒ＠ｓｐｏｎｄｔｎｇ　ｂ
ｓ＊＋ｉｍｂ１ｍ　ｍｍｂｓ％ａｎｅ＊　）との反応の
一つの成分を検出および測定するために、＊酸剤（ｔｎ
ａｒｋ［相］ｒ）でライルして指定した成分の一つを、
少なくも相手の成分を含有する反応混合物中でインキュ
ベートし、その結合する相手の物質に結びついた＃ｌｌ
酸成分、結びつかない標識成分とを分離して、最後に得
られた２つの画分のうちの少なくとも一方に含まれる標
識剤を醐足することが知られている。標識成分の２つの
画分への分布が、試料中に存在する測定すべき物質の量
の目安となる。かかる方法を行なうことのできる３ｆｉ
の系をあげると次のとおりである。
（ａ）　　％足の結合活性を持つ蛋白質としての抗体と
。
結合する物質としての甜応する抗原。とりわけこの系で
＃１定可能な物質としては、蛋白ホルモン類及びそれら
の抗体、またはビールス抗原とそれらの抗体である； ｔｂｌ　　特だの結合活性をもつ蛋白質としての抗体と
、結付する物質としてのハシテン。ここでハプテンとは
、抗体と反応するがそれらを誘導しない非蛋白４１１ｊ
［（ｐｒｏｔｅｉｎ−ｆｒｖｓ　５ｕｂｓｔａｎｅ＊　
）と定−する。とりわけこの系で測定可屈な物質はステ
ロイドホルモンとビタミン類である； （ｃｌ　　％定の結合活性を持つ蛋白質として、生体中
で受容体（ｒ・Ｃすｔｏｒ　）あるいは輸送分子（ｔｒ
ａｍｓ−ｐｏｒｔ　ｍｏｌ＠ｅｕｌ＊ｓ　）　として作
用する蛋白質、及び結合する物質としては該蛋白質と結
合する物質。
この系は例えばステロイドホルモンの測定に適している
が、また千ロキシンやトリヨーＦチロキシン、ビタミン
Ｂ１２．内生因子（１ｎｔｒｌｎｓｉｃｆａｃｔｏｒ　
）及び副腎皮質刺激ホルモンの測定にも適している。
上記の測定法で最も１壷な点は、＊酸剤を使用すること
及びＩＩ織された成分な対応する成分に結合した一分と
結合しない画分とに分離することである。
現在までに実際に用いられている方法では、放射性原子
のみがｌｌｌ識剤として用いられてきた（例１３１　　
１２５　　　１　　　５　　　６７えば　■、　Ｉ、　
　　Ｃ，Ｈ，Ｃｏ）。この方法は高感度の故に丁ぐれて
いる。しかしながら、この方法を用いるのは、市販の特
別な装置１！ｔ；４にする研究所に限定される。
分離方法は以下のように分けることができる。
すなわち、 （ａ）　　結合していない標識成分とその対応する成分
に結合した複合体の間の物理的性質の差を利用する方法
（例えばゲルＦ通、電気泳動、塩沈澱、及びデキストラ
ンで被った活性炭への吸着を利用する方法）； （ｂｌ　　前もって一方の成分を固型担体に架橋または
共有結合または物理吸着させて不溶な形態にしておく、
いわゆる固相法。
（ｅ）　　いわゆる二抗体法、ｊなわち、生じた複合体
（抗原−抗体またはハプテン−抗体の［合体）　’に’
該４１１酋体中の抗体に対する抗体の助けで沈澱させる
方法で、本方法は、抗体が関与する系にのみ公知である
。
（ａ）とｔｅｌの方法は実施が比較的面倒であり、（ｂ
ｌの方法は１反応酸分を不溶の形態にする結果、その反
応の相手に対する親和性が通常減少するという′不利益
がある。通常、高い親和性は高感度の系の試験を実現さ
せるのに必須である。
特定の結合活性を持つ蛋白質とこれに対応して結合する
物質との反応の一つの成分を検出及び定量する方法が今
や明らかとなった。すなわち、そのような成分の他に対
する既仰の結合親和力を用いる方法で、結合する物質と
酵素とのカップリング生成物の一定量と、特定の結合活
性ｉ持つ蛋白に対する抗体とを用いて不溶の形にし１反
応後反応混合物中の液相または固相の酵素活性を定量す
ることにより、測定しようとしている成分の定量な行な
うことＶ％黴とする方法である。
この方法は１％定の結合活性な持つ蛋白質として抗体な
、それに対応して結合する物質として抗原またはハプテ
ンな用いる場合しばしば用いられ。
良い結果を示す。
本文中「結合体Ｊ　（ｅｏｎｊｕｇａｔ・）及び「酵素
結合体Ｊ　（＊ｕｘｙｍｅ　ｃｏｎｊｕｇａｔ＠）とい
う飴１１、結合する物質と酵素とのカップリング生成物
と同義に用いる。
結合する物質の検出及び重壷は次の如く行なうことがで
きる。未知の試料また６ｊその一連の希釈物を、測定し
ようとする物質と酵素との結合体の既知量と特定の一合
活性を持つ蛋白質の添加した酵素結合体の量に依存する
一定量とに合する。次に特定の結合活性を持つ蛋白質に
対する不溶化にした抗体の一定量（望ましくは過剰量）
を添加して、該結合活性を持つ蛋白質と反応した全ての
酵素結合体χ、この蛋白質を通じて、これらの不溶性の
抗体に結合させる。試料中に結合する物質の−が多くな
ればなるほど、４１定の結合活性なもつ蛋白質と反応し
且つ最終的に不溶の相になる酵素結合体は少な（なる。
そのため、液相に多くの結合していない＃素結合体が残
り、このものが単に足前されることになる。
特定の結合活性を持つ蛋白質の測定は、試料またはその
一連の希釈物を、酵素結合体の既知量及び特定の結合活
性を持つ蛋白質に対する抗体の不溶化したものとインキ
ュは一トすることにより行なうことができる。結合体が
特定の結合活性を持つ蛋白質と反応したとすれば、酵素
活性は不溶の相に移り、特定の結合活性な持つ蛋白質が
試料中に多ければそれだけ、液相に残る結合する酵素結
合体が少なくなる。
上述の試薬系の感度は試薬の量を変えて（同じ比率でも
、それ以外でもよい）Ｒ化させることができる。しかし
、用いることのできる酵素結合体の量は、その＃累活性
Ｖ＊虐なく測定できなければならないという点で、下限
があり、従って試験系の感度には限度がある。とりわけ
、測定可能な酵素活性の最少値は、カップリングに用い
る＃素の性質と基質の性質、及び＃素反応のインキュば
一ジョン時間に依存している。更に、特定の結合活性を
持つ蛋白質の親和性は測定の感度に大きな影響を与える
。高感度で測定を行なうには、高い親和性を持つ特定の
結合活性を持つ蛋白質が必要である。
測定に必要な試薬の量は経験的に確定する。
結合する物質の測定のため、＃集結合体の量な酵素活性
の助けをかりて測定し、この量′４！：特定の結合活性
を持つ蛋白質の一連の希釈物とインキュイードしてこの
蛋白質の必要量を測定する。好ましくは、酵素結合体の
５０−９０％と結合する特定の結合活性を持つ蛋白質の
一定量を選ぶ。最終的に、所望の感度が本当に得られた
かど５かを。
この系の試−のもとてその物質の一連の希釈物を試験し
て―ぺる。特定の結合活性を持つ蛋白質の−ｊ足には、
酵素結合体の量に関してもう一つ考えねばならねことが
あり、それはその感度である。
その感度は無理なく測定できるようでなければならない
。
特定の結合活性を持つ蛋白質に対する不溶にした抗体は
この２つの測定タイプにおいては望ましくは過剰に添加
する。その量は予備試験で測定しておく。
本方法のすぐれた点は。
（ａｌ　　放射性同位元素による実施を酵素な用いるや
り方に置きかえることができる。このことは実験の施設
や装置を可成り小規模にするが、実験を行なう人間も高
度の技術を必要としない。その上。
放射性同位元素を用いる仕事は法律上極めて制限Ｙ受け
ている。更に、本発明による試薬は長い貯蔵に耐え、安
全性も増大している。
（ｂ）「二抗体法Ｊ　（Ｄｏｔ＋ｂｌ＠Ａｍｔｌｂ＠ｄ
ｙ　）と「固相法Ｊ　（＝　８ｏ１１ｄ　Ｐｈａａ＠）
を組み合わせた方法（以下単にＤＡＳＰ法と呼ぶ）は現
在用いられている方法に対していくつかの優れた利点を
持つ。
すなわち、この新たに見出された方法の実施（すなわち
、特定の結合活性を持つ蛋白質に対する抗体の不溶にし
た′ものの株加、インキュベーション。
遠心分離及び測定）は非常に簡単である。使用量は固相
法に比してそれ程正確でなくてよい。何故なら固相法で
は正確の量の不溶Ｍ科を用いねばならないのに対し、本
方法では過剰量のそれｔ用いればよいからである。その
上、結合する物質に対する結合活性を持つ蛋白質の親和
性は、固相法で行なう場合のように担体に結合すること
で社められることはない。本方法の別の利点は、４１定
の結合活性を持つ蛋白質とそれに結合する物質（両方共
ｍ液）の間の反応が迅速に平衡に達するということであ
る。ｌ！には、ＤＡ８Ｐ法では、不溶にした抗体（以下
免疫吸着剤と呼ぶ）ヲ、抗体な特定の結合活性を有する
蛋白として用いる全ての系（但しこれらの抗体が同種の
動物からＩｉｌ＆Ｌ、たちのである場合）で用いること
ができるという利点がある。他方、固相法で抗原または
ハプテン＋Ｓ定する場合は、抗体は不溶にしなければな
らない。
二抗体法は塩ａｌｌ［％ｐＨ等の比較的小さな変化にも
極めて鋭敏である。従ってこれらの条件の敵しい調整が
必要とされる。その上、この方法は「担体Ｊ　（ｃａｒ
ｒｉ＠ｒ　）　ｒ−グロブリンを添加しなければならず
、結果的に免疫沈＃を得るのに多量の第２の抗体を必要
とする。操作が簡単であるのみならず、ＤＡｇＰ法は「
担体」γ−グロブリンな必要とせず、而して材料を節約
することができる。
更に、この目的に利用できる適当な担体蛋白質がないの
で、輸送または受容蛋白質に対して行なう二重抗体様の
分離は可能ではないということな付は加えることができ
よう。故に本発明の方法はこの面で従来見られなかった
可能性Ｖ提供するものであろう。
ｔｃ＞　　本発明の方法は簡単に自動化できる。原理的
には、反応成分Ｙ−Ｈに一緒にするかまたはそれらを任
意の順序で系の中に加えることも可能である。しかし、
免疫吸着剤を他の反応成分のインキュは−ションの後で
加えるなら、＃１定の高い感度が得られることが判明し
た。
本発明に必要な試薬（抗原、ハプテンの結合する物質と
酵素とのカップリング生成−は公知の方法で製造できる
。一つの物質が一つまたはそれ以上のアミノ基を持ち、
他の物質が一つまたはそれ以上のカルボキシル基を持つ
時、これらの方法は、ハプテンまたは低分子の結合する
物質を１１８に結合ｊものにも用いることができる。も
し後者がカルボキシル基を持たないならば、所望の基な
分子に導入して、公知の有機化学反応を用いてカップリ
ングさぜることができる。また、架橋結合（ｂｒｌｄｇ
・）１に導入するかまたはそうしないで。
アミノ基またはカルボキシル基な一緒に結合する方法が
知られている。また、ゲルタールアルデヒド、ジフルオ
ルジニトロフェニルスルフオン及びジ及びトリクロル−
８−トリアジンのような化合物が間臘のカップリングに
しばしば用いることができる。製造した＃素結合体を、
変換されていない物質または不活性になった物質から分
離することは必要である。この目的のため、公知の生化
学的方法、すなわち有機溶媒による沈澱、ゲルー過、及
び密度勾配での遠心分離を用いることができる。
カップリンメ生成物に変換される酵素の選択は、その酵
素の多（の性質により決まる。もちろん。
その酵素が他の分子とのカップリングに対し抵抗性があ
る（すなわち１つまたはそれ以上のアミノ酸側鎖の変ｌ
りということは必須である。また非電に重要なものに酵
素の比活性がある。測定可能な＃素の効果を得るために
必要な酵素結合体量がψ俗くなればそれだけ、試験系の
感度は高くなる。
更に、活性の測定が簡単にできる＃素が好まれる。
論−に、これらの＃累は比色的に、分光光度的１こ、ま
たは螢光的に測定が可能であると考えられる。
この種の測定は、自動化に適しており、これが付加的な
利点である。
比色的には、これらの酵素は、一番目のあるいは二番目
の反応のどちらかで１色のついた物質が現れたり消えた
りする反応を触媒することで測定できる。
結合体中で酵素的に活性のある成分として働くと考えら
れる酵素としては、カタラーゼ、／２−オキシ／−４’
、β−グルコロニ／−４’、β−Ｄ−１’ルコシグーゼ
、β−Ｄ−ガラクトクダーゼ、ウレアーゼ、グルコース
オキシダーゼ、ガラクトースオキγ〆−ゼ及びアルカリ
フォスファターゼがある０ 成分と試薬との間の反応の最後に反応混合物の液相また
は一相の、または両相の＃累活性を測定ｊることができ
る。しかし、最も簡単なものは液相の＃水活性ｖｍ定す
ることである。
特定の結合活性な持つ蛋白質に対する不溶にした抗体（
これもまた本発明の方法に必須の試薬である）もまた公
知の方法で製造できる。この抗体は％特定の結合活性な
持つ蛋白質または、少なくも一部は上記蛋白質と同じ抗
原性を持つ蛋白質を精製し、それを公知の方法でそれを
得た動物以外の他の動物に注射して製造できる。処理し
た動物の血清またはそれらのガンマグロブリン画分を、
グルタールアルデヒ）ＩＩ、クロロフォルミル酸エチル
エステルのような化合物で架ｌＩするか、または固型担
体粒子に物思的に吸着させるかまたは共有結合の形成に
より化学的に結合させて不溶化することができる。固体
担体としてはセルローズ（変性してあってもなくてもよ
い）、アガロース、架橋デキストラン、ポリスチレン等
の物質がある。
これらの物質に抗体を共有結合させるには、カルボジイ
ミド、ジ及びトリクロル−８−トリアジン。
ゲルタールアルデヒド、シアノゲンプロマイＶのような
物質を用いて、例えば、ジアゾ化（ｄｌａｇｏ−ｔｍｔ
ｌｏｎ　）で行なうと効果的である。
本発明の方法の利点は、特定の結合活性を持つ蛋白質が
完全に固相に移るように、過剰の不溶性抗体を用いた場
合、充分証明される。
試薬が用いられる形態はいろいろある。反応系の酵素結
合体成分は凍結乾燥するか、バッファーに＃ｌ解するこ
とができる。また固体担体として◆例えば結合体をしみ
こませた細長いａを用いることができる。これは、必要
な特定の結合活性を持つ蛋白質にも同じように適用する
ことができる。
不溶成分は異なる寸法の粒子の形（すなわち顆粒、薄片
、棒状）またはある担体物質の細長い小片の形−にする
ことができる。
本発明の操作を実施するのには、試薬セット（ｔ・−ｔ
　ｐａｃｋ　）の形層で適用するのが好ましい。
これは主に次のものからなっている； （１）抗原、ハプテンまたは低分子の結合可能な物質と
酵素との既知量の結合体。
（２〕　　対応する量の％足の結合活性を持つ蛋白質（
抗体または、−送置白質あるいは受容蛋白質）。
（３）用いた特定の結合活性を持つ蛋白質に対する抗体
の不溶化したものの既知量。
試薬セットには、更に酵素＃ｊ定に必要な試薬と、また
試験馨行うのに必要な補助的な手段、すなわち試験管、
ピイット及び希釈＃！液を入れた容器をも含有゛させて
もよい。このような試薬セットは結合する物質の＃１定
に適しているが、また特定の結合活性を持つ蛋白質の測
定にも適しており、この４合このセットに含有されてい
る特定の結合活性ｔもつ蛋白質は用いる必要がない。
試薬セラ）は抗原またはバッテンの検出及び測定に特番
こしばしば便利に利用され、この目的のため主に次のも
の’ｖｔｖｔ、ている； ｔａｌ　　抗原またはハプテンと酵素との既知量の結合
体。
（ｂ）　　対応する量の対応する抗体。
（ｅｌ　　用いた抗体に対する抗体の不溶化したものの
既知量。
抗体の検出及び測定に試薬セットを用いる場合は、（ｂ
）に述べた抗体は不要である。
性ゴナドトロピン：　Ｈｕｍａｎ　Ｃｈｏｒｌｏｍｌｅ
　Ｇｏｎａｄｏ　−ｔｒｏｐｉｍ　）を測定して非常に
早い時期に妊娠′ｌｋ：診断するのに用いる試薬セット
である。この試薬セットはアンプル、試験管、必須成分
として凍結乾燥しに層分離した。あらかじめ定められた
下記の物質を含有するビンまたは他の容器から成ってい
る。丁なｔち。
（ａｌＨｃＧと酵素の結合体１例えばＨＣＧ−７２−オ
キシダーゼ、 ｌｂｌ　　抗−ＨＣＧ。
（ｅｌ　　抗ＨＣＧに対する抗体の不溶化したもの。
（ｄ）　　バッファーのような他の成分。
妊娠していると思われる婦人の尿のある量ｔこの試−用
キット番こ添加し、このキットの成分と共にインキュベ
ートすると、不溶性物質の混合物が生成する。上澄は残
存する可Ｍ性ＨＣＧ−酵素結合体を含有する。この残存
する可溶性ＨＣＧ−酵素結合体の量は、試験される尿中
に含まれるＨＣＧの量に依存する。この残存する）ＩＣ
Ｇ−酵素結合体の酵素活性な測定すると、その尿が妊娠
した婦人のものか否かを判定することができる。
＃素話性の＃１足に用いる好ましい方法は、酵素試薬χ
含浸させた指示剤紙に接触させることからなる。例えば
パーオキシダーゼを用いる場合。
Ｈ２Ｏ２−供与体（例えば尿素−ａｍａｍ　　）および
発色試薬（例えば０−トリジン）ｖＬみこませた指示剤
紙に接触させる。
試薬の量を各々正しく選択することで、非常に早い時期
の妊娠を判定することが可屈となり、またこのことを単
純で迅速で非常に信頼できる方法で熟練していない人に
も行なうことができるであろう。
実施例　１ 訃絨毛性ゴナト９トロピン（ＨＣＧ）の＃ｊ定（ａ）　
　ＨＣＧ　−１１ＲＰの製造 ＨＣＧ５ｍｇと西洋ワサビノーオキシダーゼ（ＨＲＰ　
）　２０１１ｆ’＆０．０５Ｍす７　ｆｌｌ　／：ツフ
ァー（ｐＨ６，２）　２ｍ１１１７に溶解した。２５％
ゲルタールアルデヒド溶液４０ｐ１を添加後、混合物を
２時間室温で振盪した。２５０．９で５分遠心分離した
後。
０．０５Ｍリン酸バッファー（ｐ）１６．２）！用いセ
ファデックスＧ−２００で分−した。最も高いノぞ一セ
ントの＃素話性がＨＣＧに対する抗体ＳＣｇ會している
一分を試験系に用いた。
（ｂ）ＨＣＧに対する抗体の製造 ＨＣＧに対する抗体をシュールズら（Ｓ・ｈｌｌｕｒｌ
ａｔ　４１．）の方法〔ムｅｔａ　Ｅｎｄｅｅｒ、　（
Ｋｂｂ　）シ２．　１２０　（１９６８）　）　　でウ
サギに一発させた。
（１１）ウサギ−ｒ−グロブリンに対する抗体の製造ウ
サギ−ｒ−グロブリンを正常なウサギ血清より１８％Ｗ
／Ｖ固体硫−ナトリウムによる沈澱で単離した。これに
対する抗体χ上述の計画によりヒツジを免疫することで
製造した。
日　　量　　　　フロイントの補薬　　　注射方法０　
０．５Ｍ９　　　　　　＋　　　　　　筋肉内１４０．
５■　　　　　　＋　　　　　　　　１２８１　　ｗ９
　　　　　　＋　　　　　　　　　ｐ４２　１　　ダ　
　　　　　　　　　　　静脈内５６１　　ａｉ！　　　
　　−＃ １０日目にこのヒ゛ンジを採血した。
（ｄｌ　　免疫吸着剤〔ヒツジ−抗−（ウサギ−ｒ−グ
ロブリン）〕セルローズの製造 （ｅ）に述べたヒツジ血清のｒ−グロブリン画分ｖ１６
％Ｗ／ｖ固体＊ｔｉｔナトリウムで沈澱させて！Ｉｌ製
した。洗った後、沈澱物ｔ、蛋白の最終ａｌｌｆが１０
ｍ９／−になるようにｐＨ８，６０）　０．０５Ｍｄｔ
つ酸バッファーにとった。ｍ−丁ミノはンジルオキシメ
チルセルローズ３５０１１ｇ４蒸留水５９ｍにｆｌｌ濁
させ、３６％塩＠１０ｍ１７４’加ｔ、Ｏ’Ｃ１？１０
％ＮａＮＯ２ｆｌＭ　　１０Ｎ１１に滴下してジアゾ化
した。
懸濁物を遠心分離し、洗い、そして沈澱物をｐｉ８．６
の０．０５Ｍホウ酸ナトリウム４３ｗＪに再び懸濁させ
た。それから調製したｒ−グロブリンの痔液７ＩＬＬ１
！を添加した。混合物’ｔ２６時間４℃で攪拌し、而し
て遠心分離し、ｐＨ６，０の０．０２Ｍリン酸バッファ
ーで洗った。
（・）ＨＣＧの測定 ＨＣＧＹｐＨ６，０（７）０．０２Ｍリン酸バッファー
に希釈し１こ一遍の希釈物（３２−１６−８−４−２−
１−０，５−ＯＩＵ／ｄ）を調製した。これは２％Ｗ／
Ｖの正常なヒツジ血清を含有している。
ＨＣＧＹ言Ｍする試料それぞれ０．５ｄを、ウサギ−（
抗−ＨＣＧ）血清０．１−及びＨＣＧ−ＨＲＰ結合体０
．１　ｍ　（両方共適当に希釈する）と共に室温で３０
分間インキュベートした。それがら（ｄｌに従って、、
ａｌｌ製した免疫＠層剤（１０岬／ｄン０，３ｍを添加
し、生成した混合物な室温で１時間回転させる。遠心分
ｔｍ後、上ｆ液のｍ素話性を―ぺるために上澄液０．ａ
ｌｌを基質（３０％Ｈ２Ｏ２１０）１１及び５−アミノ
サソチルＭ　２０　Ｍ９ｋ　ｐＨ６，０（Ｄ　Ｏ，０２
Ｍリン酸バッファー１５０ｉｕに爵解したもの）１．５
１と混合し、３０分後２５℃で４６０　ｎｍでの吸光度
を測定して前記酵素活性Ｖ足置した。
この方法では、試料中ＨＣＧのａｌｌ［が０．５乃至Ｉ
ＩＵ／−であってもそれらを検出することができること
が判明した。この方法ではまた尿の試料１！を調べるこ
とができ、本試験は妊販の判定に適している。現在用い
られている試験法、血対“＠果反応組、ＪＪ：試験との
関係は良好だった。前もってインキュベーション処理を
行うことでこの系の感ＩＪＩ上げることができることが
判明した。ここで、最初に試料だけを抗血清とインキュ
ば−ションに何し、次にＨＣＧ−ＨＲＰ結合体を添加し
た。
実施例　２ インシュリンと抗インシュリンの測定 （鳳）インシュリン−（／＃ルコースオキシダーゼ）の
製造 ブタインシュリン５■とダル−コースオキシダーゼ２５
１９ｉ　ｐＨ６，５ノ０．０５　Ｍ　ＩＪ　７酸ノ５７
７ｆ　−２ｗＬｌに＃Ｉ解した。これに２５％ダルター
ルアルデヒド溶液５μｊ１に：添加し、この後この混合
物を室温で９０分間振盪した。混合物ｔ／ｐＨ６，５の
０．０５Ｍリン酸ノ署ツファー中セファデックスＧ−２
００で分−した。高いパーセントの酵素活性がインシュ
リンに対する抗体に結合した画分Ｙ：拭威系に用いた。
（ｂｌ　　インシュリンに対する抗体の製造モルモット
１０＠１体に一週一〆完全なフロイン◆なしでインシュ
リンｌｌｖづつｔ静脈注射した。
それから２遍閣後実−動物を採血した。同時裔こおこる
低血楯症はグルコースの腹紋円注射でおさえた０ （ｃ）　　モルモットγ−グロブリンに対する抗体の製
造 モルモットγ−グロブリンな飽和硫酸アンモニウム＃！
液１各量をモルモット血清２容駿に加えて製造した。生
じた沈澱物な３３％飽和硫酸アンモニウム溶液で２に洗
い、それから生理的食塩水にとった。ヒツジχ、０．５
，１及び２Ｍ９とｒ−グロブリンの投与量を増加させな
がら免疫した。免疫原は完全なフロイントの補薬と混ぜ
、注射は２週間おきに行なった。最後の注射から２週関
後生鳩食塩水にｒ−グロブリン２１ｆｔ’＃！解したも
のな更に与え、それから１週間後に実験動物を採血した
。
（ｄ）　　モルモットｒ−グロブリンに対する不溶化抗
体の製造 ｉ　りＯ結Ｊ＆−ｔ！Ａ’Ｏ−ズ１０　Ｎ　ｋ　２．５
％Ｗ／　ＶＣＮＢ、　　浴液４００−に攪拌しながら添
加して活性化し、その後ｌＮＮａＯＨで９１％−１０，
５にし。
このまま２分間おく。それからセルローズな氷水と０．
１　Ｍ　　ＮａＨＣＯ３”’ＱｆｋＤた。ヒツジ−抗（
モルモットｒ−グロブリン）血清１０１１１ＧＣＮ＊、
Ｂｏａｌ、６ｇ’ｋｍ加した。室温で１時間攪拌後洗澱
物を遠心分離し、１６％Ｗ／Ｖ　ＮａｘＢＯａ　溶液２
０１で２回洗い１次いで０．ＩＭ　　ＮａＨＣＯ暴１０
−にとる。活性化したセルローズな、０．１Ｍ　　Ｎａ
ＨＣＯｓＭ［４０１Ｌｌとｒ−グロブリン溶液ｌＯ−と
混ぜた。
この１１１４１ｆｔを４℃で４０時間−転させ、つづい
て０．５　Ｍ　　ＮａＨＣＯｉ　　５００−で２ｄ、ｐ
Ｈ１，１の０．０５　Ｍクエン−塩５００ｄで２回、ｐ
Ｈ６，２の０．０５Ｍリン酸塩５００−で２１１１！ｌ
洗５゜（・）インクニリンに対する抗体の＃１足適当に
希釈したインシュリン−（グルコースオキシダーゼ）ｏ
、１ｉｌＹ：、モルモット抗−インシュリン血清の一遍
の希釈物０．４−と４時間インキュ（−トシた。一連の
希釈物はｐＨ６，０の０．０５Ｍリン酸）；ツファーで
一度した。それから免疫吸着剤（１５Ｍ９／Ｍｌ　）　
０．３．ｍＪトバツファ−０，２ｄＹ添加し、混合物を
一晩４℃で回転させた。遠心分ｌｌ１ｉ優、上置の酵素
活性を、このもの０．５１１１７Ｙ基質２．５−と３０
分インキュば−）Ｌ、４６０ａｍでの吸光＆を測って測
定した。基質は、ｐＨ６，０の０．０５　Ｍリン酸ノ之
ツファ−２，５−に対してグルコースｓｏｑ、パーオキ
シダーゼ１０μＩ及び５−４ミノサリチル−１ｍ９Ｙ含
有させたものである。
この系の方法で、異なる血清の抗体の内容量を相互に比
較できろ。比較する点として、全体の結合できる酵素活
性の５０％が結合する血清の希釈物がある。
（ｆｌ　　インシュリンの一１足 一連のインシュリンの希釈物ｇ、２ａＪ４抗−インシニ
ーインシュリン血清４と２時間インキュベートした。
抗−インシュリン血清は、後で加えられる＃業績合体の
６０％に結合するように希釈されている。
それからインシュリン−（グルコースオキシダーゼ）Ｑ
、ｌｄＱ対応する希釈物に添加し、４時間インキュベー
トした。最後に免疫吸着剤（１５■／ｄ）０．３１Ｌｔ
を添加した。混合物を一晩４℃で回転させた。遠心分＃
ｌ醜上澄の酵素活性ｔ’（・）に述べたように測定した
。
測定感度は（これは用いた抗血清によるのだが）ナノグ
ラム（ｎａｉｔｏ、５ｒａｍ　）の範囲で、２Ｏ−Ｚｏ
。
＊　ｆｌ　／　ｍ　、すなわち０．５〜２．５　ｍ　Ｖ
Ｊ／　ｄであった。
１１且−１ エストラジオールの測定 神）　エストラジオール−１７−サクジニルーＨＲＰの
線速 エストラジオール−１７−ヘミサクシネート５０■とト
リーｎ−プナル了ミンｇ、□Ｂｊｖ９オキサン２．５１
１ｊｔＣ＃！ｊ！＊Ｌ７：。冷却シｙＪｌ液（２℃）に
イソブチルクロロカーボネート１５μｊを添加した。３
０分後、このｍ液ｔ、水酸化ナトリウムでｐ）１９．５
に會わ甘たジオキサン／水混合吻（２：　３　）　７．
５−に溶解した西洋ワサビパーオキシダーゼ（ＨＲＰ）
１００”ｔと混合した。この連数を２℃で４時間攪拌し
、それから１８時間透析した。透析物のＰＩｎ４．６に
合わせた後生じた沈澱物を遠心分離して洗浄し、ｐ）１
Ｂに合わせた蒸城水５−にとった。この物質ｔｌ！にア
セトン１〇−で２錦沈澱さセてｎ１Ｉｌｌシた。最後に
得られた生成物を、ｐＨ７，８の０．０５Ｍリン酸バッ
フ丁−１〇−中に取出す。
（ｂｌ　　エストラジオール−１７−サックニル−８８
ムの製造 製造番工実ｍ例３の（ａ）に述べた混合無水物法で行な
った。このｇａは、エストラジオール−１７−ヘミサク
シネート１００■とウシ血清アルブミン（Ｂ８ム）１５
０■より出発した。
（ｅ）　　エストラジオールに対する抗体の製造ヒツジ
に、完全なフロイントの補薬に溶解したエストラジオー
ル−１７−サクジニルーＢ８ム４■１４週間に一度注射
した。通常の間隔をおいてヒツジを採血した。血清は不
溶にしたＢ８ムで吸収さ（た。
（ｄｉ　　ヒツジ−゛Ｔ−グロブリンに対する抗体の製
造ヒツジ−γ−グロブリンを実施例１に述べたように、
但し今回はｌム％Ｗ／Ｖｌ敏ナトリウムで調製した。ウ
サギを１次に述べろ計画に従ってこのヒツジ−ｒ−グロ
ブリンで免疫した。
日　　　　量　　　　フロイントの禰桑　　注射法０２
００μｇ　　　　　十　　　　　筋肉内１４　　４００
μｆｉ　　　　　　＋　　　　　　　　ｔ２８　　　８
００　　μ　ｉ　　　　　　　　　　　　＋　　　　　
　　　　　　　　　ｇ４２　８００μ９　　　　　　　
　　　　　静脈内蛾後の注射から２週間して実験動物を
採血した。
神）　免疫吸着剤〔ウサギ−抗（ヒツジ−ｒ−グロブリ
ン）〕−セルローズの製造 （ｄ■こ述ぺた抗血清のｒ−グロブリン画分を、１８％
Ｗ／　Ｖ　　Ｎａ２８０４　で沈澱させてＩＩ製した。
得られた生成物を実施例１に述べたガーピッヒ法（Ｇｕ
ｒｖｉｅｈ　Ｍ＠ｔｈｏｄ　）　　でセルローズに結合
させた。
ｌｆ）　　エストラジオールの測定 免疫反応を２％Ｂ８Ａｔ’ｔｉｉするｐＨ６，０（ＤＯ
，０２Ｍりン鍍バッファー中で行なった。
試料ｏ、ｓ＊ｙ所望の希釈でヒツジ−抗−エストラシオ
ール血清０．１−と混合した。′Ｎ、温で３０分インキ
ュイージョン後、適当に希釈したエストラジ、ｔ　−ｉ
ｖ　−１７−サ９　シーｔｖ　−ＨＲＰ　Ｏ，Ｉ　ｍｌ
’に添加し、その後もう一度家温で３０分インキュは−
トした。それから、免疫吸着剤の一４？［（３０ダ／Ｍ
ｌ）ｏ、ａｓｙｖ添加し、その混合物を室温で２時間回
転させた。それから遠心分−で液相と固相ｔそれぞれ分
離し、上澄の酵素活性Ｖ実施例１に述べたように測定し
た。ヒツジ−抗−エストラジオール血清は、用いるエス
トラジオール−】７−サクジニルーＨＲＰの品質により
１：１６００乃至１：１２８００の希釈で用いることが
できる。
抗血清１：　１２，８００の希釈では、エストラジオー
ル譲ｆ１０　ｎ１１７ｍを試料中に検出できる。
エストリオールとエストロンはこの系で交叉反応を起こ
す。
実施例　４ コルチゾール及びコルチコイド−結合グロブリンの６４
１１定 （ａ）　　コルチゾール−２ｌ−（ガラクトースオキシ
ダーゼ）の車速 コルナシ−ルー２１−ヘミサクシネート５０１１ｇとガ
ラクトースオキシダーゼ１００ダｔ１′実施例３の（ａ
）に述べた混合無水物法で結合させる。
（ｂｌ　　コルチコイド−結合グロブリン（ＣＩＩＧ）
を、ＤＥＡＥ−セルローズと水酸化アノＺタイトの１１
にりａマドグラフィーｌこ何して人血清から単離した。
これに対する抗体を、完全なフロイントの補薬に溶かし
たＣＢＧ５００μＩを１４日おきにウサギに注射して製
造した。３ヶ月ｔｌｋ実験動物にＣＢＧ１１％？Ｙ注射
し、その２週間４１にに採血した。
＋ｅ）　　実施例３に述べたように、抗−ＣＢＧ血清の
γ−グロブリンー分子ｍ−丁ミノ纜ンジルオキシメチル
セルローズに結合させた。
（ｄ）　　コルチゾールの測定 コルチゾールを含Ｍする試料（Ｉｌｉ準＃ｇ）０．５−
を塩化メチレン３−で２回抽出し、抽出−分を合せて蒸
発乾固した。残留物’＠　ｐＨ６，２の０．０５Ｍリン
リンフファー０．５−にとり、同じノ２ツファ　　−に
適当なｌ１１度で溶かしたＣＢＧ溶敵０．１−と混合し
、４℃で３０分インキユイートシた。それから、適当に
希釈したコルチゾール−２ｌ−（ガラクトースオキシダ
ーゼ）０．１−と、（Ｃ）で１４製した５■／１の−に
の免疫吸着剤ｏ、ａｓｔｙ添加した。
得られた混合物ｖ４℃で２時間回転し、遠心分離し、そ
の後上澄の酵素活性を測定した。この目的のため、上記
上澄０，５ｗＬＩＹ、Ｄ−ガラクトース１００ダ、５−
丁ミノサリチルａ１２０Ｍ９及びノＺ−オキシダーゼ１
０μｇをｐＨ６，０の０．０２Ｍリンリンフファー１５
０−に溶解した基質１．５−に添加し、（９）分後に４
６０　ａｍの吸光［を測定した。
０．４ｌｇ〜のＳ度のＣＢＧと、ステロイド類を添加し
なくとも酵素結合体の８０％が免疫吸着剤に結合する根
の量のコルチゾール−２ｌ−（ガラクトースオキシダー
ゼ）を用（また場合、３乃至３０ｎ９のコルチゾールが
１１１１Ｍ可能であることが判明した。
１ｓ）ＣＢＧの測定もまた上記の試薬を用ｔ’ｎ、＋’
ｘ可１１１！である。０−１２８０ｎｊｌ／−に希釈し
た一連のトランスコルチン０．５１を、適当に希釈した
コルナシ−ルー２１−（ガラクトースオキシダーゼ）０
．２−と１５分間インキュベートした。それ力１ら免疫
吸着剤の＃ＩＡ濁液（５ダ／ｄ）０．３１＋ｊｔ／添加
し。
その混合物を１５分間回転した。２つのインキュば一ジ
ョン処理は４℃で行なった。それから上澄の＃素話性’
３ｆｆ　（ｄ）に述べたようにして測定した。この試験
系の感度は５０　！Ｉ＆　／−であることが判明した。
実施例　５ ピン容器中に以下の試薬Ｙ分離した層に凍結乾燥した。
（１）実施例１の（ａ）に述べた免疫吸着剤の層温ｗｒ
ｉｔ＜１０ダ／ｄ）０．３−０ （３）実施例１の（亀）に述べたＨＣＧ−４１ＲＰ０．
１１ｊ（４）１％マンニトールｆｉ−ＱＯ，１Ｉｌｊの
２番目の層（５）実施例１のｔｂ）に述べたウサギ（抗
−ＨＣＧ）血清０．１１ この凍結乾燥した混合物に、尿試料０．５１１４！と蒸
留水０．５１１１を添加し、ｌＯ分後止澄の酵素活性ｔ
。
尿素−水累ノＺ−オキシダーゼと０−トリジンｔしみこ
ませた細長い紙で測定した。
尿試料が妊娠した婦人のものであれば（）２ＩＵＨＯＧ
／１１７　　）、５分以内に層液は實変し、尿が妊娠し
た婦人のものでない時は、同じ時間内に変化は起こらな
い。

Claims (1)

    【特許請求の範囲】
  1. （１）　　抗体と、抗体に対して親和力を有する抗原お
    よびハプテンからなる群から選択される結合する物質と
    の反応を利用して、抗体を含む流体デンプル中の抗体を
    検出及び定量する方法であって、 イ、誼結合する物質と酵素との結合体を用い、口、定量
    すべき抗体に対する抗体の不溶化され九ものを用い、 ハ、前記サンプルと前記イ及び口の試県とを混合して反
    応させ、 二０反応混合物を液相と固相とに分離し、ホ１分離され
    たいずれかの一方のＩＩＯ酵素活性を測定することによ
    り、前記抗体の量を決定する、 ことを特徴とすゐ前記検出及び定量する方法。
JP57193265A 1970-12-28 1982-11-02 抗体を検出及び定量する方法 Pending JPS58117455A (ja)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
NL7018838 1970-12-28
NL707018838A NL154599B (nl) 1970-12-28 1970-12-28 Werkwijze voor het aantonen en bepalen van specifiek bindende eiwitten en hun corresponderende bindbare stoffen, alsmede testverpakking.

Publications (1)

Publication Number Publication Date
JPS58117455A true JPS58117455A (ja) 1983-07-13

Family

ID=19811894

Family Applications (3)

Application Number Title Priority Date Filing Date
JP724140A Pending JPS5834783B1 (ja) 1970-12-28 1971-12-27
JP57193266A Pending JPS58117456A (ja) 1970-12-28 1982-11-02 特定の結合活性を持つ蛋白質とこれに対応して結合する物質との反応の一つの成分を検出及び定量する試薬セツト
JP57193265A Pending JPS58117455A (ja) 1970-12-28 1982-11-02 抗体を検出及び定量する方法

Family Applications Before (2)

Application Number Title Priority Date Filing Date
JP724140A Pending JPS5834783B1 (ja) 1970-12-28 1971-12-27
JP57193266A Pending JPS58117456A (ja) 1970-12-28 1982-11-02 特定の結合活性を持つ蛋白質とこれに対応して結合する物質との反応の一つの成分を検出及び定量する試薬セツト

Country Status (20)

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US (1) US3839153A (ja)
JP (3) JPS5834783B1 (ja)
AT (1) AT320145B (ja)
AU (1) AU467394B2 (ja)
BE (1) BE777309A (ja)
BR (1) BR7108553D0 (ja)
CA (1) CA964560A (ja)
CH (1) CH557030A (ja)
DE (1) DE2164768B2 (ja)
DK (1) DK150690C (ja)
EG (1) EG11604A (ja)
ES (1) ES398372A1 (ja)
FI (1) FI54034C (ja)
FR (1) FR2120835A5 (ja)
GB (1) GB1348938A (ja)
IL (1) IL38371A (ja)
IT (1) IT965020B (ja)
NL (1) NL154599B (ja)
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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP4888387B2 (ja) * 2005-11-18 2012-02-29 三菱電機株式会社 エレベータのかご照明装置

Families Citing this family (850)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
USRE31006E (en) * 1968-09-24 1982-08-03 Akzona Incorporated Process for the demonstration and determination of reaction components having specific binding affinity for each other
NL171930C (nl) * 1972-05-11 1983-06-01 Akzo Nv Werkwijze voor het aantonen en bepalen van haptenen, alsmede testverpakkingen.
US3790447A (en) * 1972-07-05 1974-02-05 Abbott Lab Streptococci diagnostic method
US4239746A (en) * 1973-06-30 1980-12-16 Dezso Istvan Bartos Complement fixation test employing reactants in a disposable package
DE2333434C3 (de) * 1973-06-30 1978-04-06 Istvan D. Dr. 5024 Pulheim Bartos Verfahren zur Durchführung serologischer Untersuchungen nach dem Prinzip der Komplementbindungsreaktion und gebrauchsfertige Schnelltestpackung hierfür
US3935074A (en) * 1973-12-17 1976-01-27 Syva Company Antibody steric hindrance immunoassay with two antibodies
GB1508132A (en) * 1974-05-20 1978-04-19 Technicon Instr Analysis of biological fluids
US4040907A (en) * 1974-06-20 1977-08-09 Syva Company Iodothyronine enzyme conjugates
US4001087A (en) * 1974-10-10 1977-01-04 The United States Of America Affinity labelling enzymes with esters of aromatic sulfonic acids
FR2288312A1 (fr) * 1974-10-14 1976-05-14 Pasteur Institut Procede de dosage immunoenzymatique de la progesterone
US4002532A (en) * 1974-10-21 1977-01-11 Weltman Joel K Enzyme conjugates
SE388694B (sv) * 1975-01-27 1976-10-11 Kabi Ab Sett att pavisa ett antigen exv i prov av kroppvetskor, med utnyttjande av till porost berarmaterial bundna eller adsorberande antikroppar
NL7501215A (nl) * 1975-02-01 1976-08-03 Akzo Nv Methode voor het aantonen en bepalen van een antigeen of antilichaam.
US4629688A (en) * 1975-04-28 1986-12-16 Miles Laboratories, Inc. Homogeneous specific binding assay method
US4230797A (en) * 1975-04-28 1980-10-28 Miles Laboratories, Inc. Heterogenous specific binding assay employing a coenzyme as label
USRE32696E (en) * 1975-09-04 1988-06-14 Akzona Incorporated Enzymatic immunological method for determination of antigens and antibodies
US4474878A (en) * 1975-09-29 1984-10-02 Cordis Laboratories, Inc. Sandwich EIA for antigen associated with hepatitis
US4642285A (en) * 1975-09-29 1987-02-10 Diamedix Corporation Sandwich EIA for antigen
SE7610683L (sv) * 1975-09-29 1977-06-10 Cordis Corp Metod for bestemning av nervaron av ett antigen associerat med hepatit
JPS5272284A (en) * 1975-12-12 1977-06-16 Dainippon Pharmaceutical Co Enzymeeimmunoassay reagent
US4045384A (en) * 1976-07-23 1977-08-30 The Dow Chemical Company Method for forming an amide bond between a latex and protein
GB1549069A (en) * 1976-12-10 1979-08-01 Erba Farmitalia Enzyme linked immunoassay
US4407943A (en) * 1976-12-16 1983-10-04 Millipore Corporation Immobilized antibody or antigen for immunoassay
US4200690A (en) * 1976-12-16 1980-04-29 Millipore Corporation Immunoassay with membrane immobilized antibody
JPS5399319A (en) * 1977-02-09 1978-08-30 Hidematsu Hirai Novel qualitative and quantitative detecting method and detecting body for antgenic substance
IT1114861B (it) * 1977-05-12 1986-01-27 Sclavo Inst Sieroterapeut Metodo per la determinazione del contenuto di costituenti di fluidi biologici e mezzi adatti allo scopo
US4233402A (en) * 1978-04-05 1980-11-11 Syva Company Reagents and method employing channeling
AU531777B2 (en) * 1978-04-05 1983-09-08 Syva Co. Label/solid conjugate immunoassay system
US4318980A (en) * 1978-04-10 1982-03-09 Miles Laboratories, Inc. Heterogenous specific binding assay employing a cycling reactant as label
US4492751A (en) * 1978-04-10 1985-01-08 Miles Laboratories, Inc. Heterogenous specific binding assay employing an enzyme substrate as label
US5605800A (en) * 1978-04-13 1997-02-25 Institut Pasteur Method of detecting and characterizing a nucleic acid or a sequence of the latter, and enzymatic reactant for the application of this method
FR2422956A1 (fr) * 1978-04-13 1979-11-09 Pasteur Institut Procede de detection et de caracterisation d'un acide nucleique ou d'une sequence de celui-ci, et reactif enzymatique pour la mise en oeuvre de ce procede
JPS5510590A (en) * 1978-05-04 1980-01-25 Wellcome Found Enzyme immunity quantity analysis
JPS5539702A (en) * 1978-08-30 1980-03-19 Takeda Chem Ind Ltd Method of measuring enzyme immunity of pancreas glucagon
FR2440555A1 (fr) * 1978-10-31 1980-05-30 Maes Roland Perfectionnements aux procedes de determination de substances montrant entre elles des affinites de liaison specifiques, par des tests faisant intervenir une phase solide
US4260678A (en) * 1979-02-23 1981-04-07 Corning Glass Works Determining creatine kinase isoenzmes via immobilized antibody-isoenzyme complexes
JPS55152459A (en) * 1979-05-18 1980-11-27 Yamasa Shoyu Co Ltd Method and reagent for measuring quantity of cyclic nucleotide
US4289748A (en) * 1979-05-31 1981-09-15 United States Of America Ultrasensitive enzymatic radioimmunoassay method
DE2930706A1 (de) * 1979-07-28 1981-02-05 Medac Klinische Spezialpraep Verfahren zum nachweis von erregerspezifischen antikoerpern
US4299916A (en) * 1979-12-26 1981-11-10 Syva Company Preferential signal production on a surface in immunoassays
US4394391A (en) * 1980-02-19 1983-07-19 Thorell Jan Ivan Radioimmunoassay reagents
US4322495A (en) * 1980-03-11 1982-03-30 Minnesota Mining And Manufacturing Co. Immunoassay
SE8003732L (sv) * 1980-05-19 1981-11-20 Pharmacia Diagnostics Ab Sett vid bestemningsmetoder involverande biospecifika affinitetsreaktioner
EP0040728A1 (en) * 1980-05-19 1981-12-02 Pharmacia Diagnostics Ab An improvement in and relating to assaying methods involving biospecific affinity reactions
JPS5714748A (en) * 1980-07-01 1982-01-26 Dainippon Pharmaceut Co Ltd Kit for quantitative determination of valproic acid and its method for quantitative determination
US4323647A (en) * 1980-10-15 1982-04-06 University Of Miami Steric hindrance enzyme immunoassay
DE3048884A1 (de) * 1980-12-23 1982-07-15 Boehringer Mannheim Gmbh, 6800 Mannheim Verfahren zur enzymimmunobestimmung in heterogener phase
ES511156A0 (es) * 1981-04-13 1983-08-01 Hoechst Co American Un metodo de determinar la presencia de un antigeno en un medio liquido sospechoso de contenerlo.
US4604365A (en) * 1981-06-02 1986-08-05 Electro-Nucleonics, Inc. Immunoprecipitation assay
WO1982004323A1 (en) * 1981-06-02 1982-12-09 O Neill Sean Immunoprecipitation assay
US4506009A (en) * 1982-03-30 1985-03-19 University Of California Heterogeneous immunoassay method
US4535057A (en) * 1982-07-26 1985-08-13 Amf Incorporated Immunoassay employing monoclonal herpes simplex antibody and biotin-avidin detection system
US4515890A (en) * 1982-11-08 1985-05-07 Abbott Laboratories Immunoassay of terminal deoxynucleotidyl transferase
US4650751A (en) * 1983-04-29 1987-03-17 Technicon Instruments Corporation Protected binding assay avoiding non-specific protein interference
US4778752A (en) * 1983-10-11 1988-10-18 Scripps Clinic And Research Foundation Receptors specific for hapten-modified self proteins
US4937199A (en) * 1985-03-18 1990-06-26 The Royal Free Hospital School Of Medicine Detection of viruses and antibodies
JPS62148857A (ja) * 1985-12-24 1987-07-02 Toyo Soda Mfg Co Ltd 免疫測定用材料の製造方法
EP0247730A3 (en) * 1986-04-28 1989-04-12 Antibody Technology Limited Antibodies, their preparation and use and products containing them
DE3636724A1 (de) * 1986-10-29 1988-06-23 Joern Dr Med Kekow Enzymimmunoassay (elisa) zur insulinbestimmung im mikrotiterplattensystem
US6528292B1 (en) * 1987-05-29 2003-03-04 Aventis Pharmaceuticals Holdings Inc. Derivatized polystyrene and other polymer supports for spectroscopic studies
US5256372A (en) * 1987-11-06 1993-10-26 Idexx Corporation Dipstick test device including a removable filter assembly
US5374524A (en) * 1988-05-10 1994-12-20 E. I. Du Pont De Nemours And Company Solution sandwich hybridization, capture and detection of amplified nucleic acids
US5137804A (en) * 1988-05-10 1992-08-11 E. I. Du Pont De Nemours And Company Assay device and immunoassay
US5614504A (en) * 1990-08-01 1997-03-25 The University Of South Florida Method of making inosine monophosphate derivatives and immunopotentiating uses thereof
US5965379A (en) * 1991-07-19 1999-10-12 Cytimmune Sciences Inc. Method for measuring endogenous cytokines
ATE184703T1 (de) * 1991-07-19 1999-10-15 Cytimmune Sciences Inc Verfahren und testsatz zur messung von endogenen zytokinen
US5464749A (en) 1991-07-22 1995-11-07 Bayer Corporation Immunoassay of free substances in biological fluids
US5874423A (en) * 1992-09-10 1999-02-23 Yissum Research Development Co. Of The Hebrew University Of Jerusalem Digitalis-like compounds
US5637467A (en) * 1992-10-13 1997-06-10 Behringwerke Ag Heterogeneous assay using a pendulous drop
WO1995034640A1 (en) * 1994-06-13 1995-12-21 Henry Ford Health System A novel neuronal-neonatal gene: neuronatin
US5843673A (en) * 1994-10-25 1998-12-01 Curators Of The University Of Missouri Method of screening for endometriosis
US6531277B2 (en) 1994-10-25 2003-03-11 The Curators Of The University Of Missouri Endometriosis-specific secretory protein
US20030152909A1 (en) * 1994-11-16 2003-08-14 Mitrani Eduardo N. In vitro micro-organs, and uses related thereto
US5607565A (en) * 1995-03-27 1997-03-04 Coulter Corporation Apparatus for measuring analytes in a fluid sample
US5777093A (en) * 1995-05-16 1998-07-07 Ramot-University Authority For Applied Research & Industrial Development Ltd. cDNAs associated with ataxia-telangiectasia
US5858661A (en) 1995-05-16 1999-01-12 Ramot-University Authority For Applied Research And Industrial Development Ataxia-telangiectasia gene and its genomic organization
US5728807A (en) * 1995-05-16 1998-03-17 Ramot-University Authority For Applied Research And Industrial Development, Ltd. Mutated proteins associated with ataxia-telangiectasia
US6096508A (en) * 1995-08-16 2000-08-01 Kirkegaard & Perry Laboratoies, Inc. Method of reducing background in biotin-based assays
US5840322A (en) * 1996-12-19 1998-11-24 Ramot-University Authority For Applied Research & Industrial Devel. Ltd. Anti-oral-microbial adhesion fraction derived from vaccinium
AU9110798A (en) * 1997-08-21 1999-03-08 Quark Biotech, Inc. Hypoxia-regulated genes
US7973156B2 (en) 1997-08-21 2011-07-05 Quark Pharmaceuticals Inc. Hypoxia-regulated genes
US6284496B1 (en) 1997-10-03 2001-09-04 University Of South Florida DNA vector for determining the presence of out-of-reading-frame mutations
CA2307345A1 (en) 1997-10-17 1999-04-29 University Of South Florida Method to diagnose and monitor cellular immune deficiencies
US7687057B2 (en) 1998-01-09 2010-03-30 Yissum Research Development Company Of The Hebrew University Of Jerusalem In vitro micro-organs, and uses related thereto
AU770896C (en) 1998-09-29 2006-09-07 Gamida Cell Ltd. Methods of controlling proliferation and differentiation of stem and progenitor cells
US20080206206A1 (en) 1998-05-07 2008-08-28 University Of South Florida Bone marrow-derived neuronal cells
US6344443B1 (en) 1998-07-08 2002-02-05 University Of South Florida Peptide antagonists of tumor necrosis factor alpha
US7605149B1 (en) 1998-07-13 2009-10-20 University Of South Florida Modulation of the phospholipase A2 pathway as a therapeutic
US6187563B1 (en) 1998-08-07 2001-02-13 Yale University βIV-spectrin-polypeptides and nucleic acids encoding same
ES2338405T3 (es) 1999-02-04 2010-05-07 Pluristem Ltd. Metodo y aparato para mantenimiento y expasion de celulas madre hemopoyeticas y/o celulas progenitoras.
US6440682B1 (en) * 1999-05-28 2002-08-27 Detroit R&D Inc. Detection of hypertension using immunoreactive metabolic products
US6787318B1 (en) 1999-06-01 2004-09-07 Roskamp Research Institute, Llc Assay for evaluating the therapeutic effectiveness of agents in reducing Alzheimer's disease pathology
US7534605B2 (en) * 1999-06-08 2009-05-19 Yissum Research Development Company Of The Hebrew University Of Jerusalem CD44 polypeptides, polynucleotides encoding same, antibodies directed thereagainst and method of using same for diagnosing and treating inflammatory diseases
CA2377541A1 (en) * 1999-06-25 2001-01-04 Eduardo N. Mitrani Method of inducing angiogenesis by micro-organs
US6875582B1 (en) 1999-08-19 2005-04-05 Omniscience Pharmaceuticals, Inc. Methods and targets of antibiotic resistance
US7811981B2 (en) * 1999-08-30 2010-10-12 Yissum Research Development Company Of The Hebrew University Of Jerusalem Methods of and compositions for inhibiting the proliferation of mammalian cells
US7101839B1 (en) 1999-08-30 2006-09-05 Yissum Research Development Company Of The Hebrew University Of Jerusalem Methods of and compositions for inhibiting the proliferation of mammalian cells
US6998232B1 (en) 1999-09-27 2006-02-14 Quark Biotech, Inc. Methods of diagnosing bladder cancer
CA2392060C (en) * 1999-11-19 2007-07-10 Yissum Research Development Company Of The Hebrew University In Jerusalem Aspergillus niger beta-glucosidase gene, protein and uses thereof
US6544506B2 (en) 2000-01-05 2003-04-08 Yeda Research & Development Co. Ltd. Veto cells effective in preventing graft rejection and devoid of graft versus host potential
US7385106B2 (en) * 2000-01-24 2008-06-10 Ramot At Tel Aviv University Ltd. Plants tolerant of environmental stress conditions, methods of generating same and novel polynucleotide sequence utilized thereby
US20040191260A1 (en) * 2003-03-26 2004-09-30 Technion Research & Development Foundation Ltd. Compositions capable of specifically binding particular human antigen presenting molecule/pathogen-derived antigen complexes and uses thereof
IL151860A0 (en) 2000-03-27 2003-04-10 Technion Res & Dev Foundation Single chain class i major histocompatibility complexes, constructs encoding same and methods of generating same
IL135884A (en) * 2000-04-30 2005-08-31 Yissum Res Dev Co Method for measuring non-transferrin bound iron
HUP0300810A2 (hu) 2000-07-20 2003-08-28 M.G.V.S. Ltd. Mesterséges értranszplantátum, valamint ennek létrehozása és alkalmazása
US20040037828A1 (en) 2002-07-09 2004-02-26 Bar-Ilan University Methods and pharmaceutical compositions for healing wounds
DK2359854T3 (da) 2000-08-08 2013-11-04 Technion Res & Dev Foundation Farmaceutiske sammensætninger og fremgangsmåder egnet til modulering af angiogenese
US20030114410A1 (en) 2000-08-08 2003-06-19 Technion Research And Development Foundation Ltd. Pharmaceutical compositions and methods useful for modulating angiogenesis and inhibiting metastasis and tumor fibrosis
EP1683874A3 (en) 2000-08-29 2006-10-11 YEDA RESEARCH AND DEVELOPMENT Co. LTD. Methods of isolating genes encoding proteins of specific function and of screening for pharmaceutically active agents
US20070281037A9 (en) * 2000-09-28 2007-12-06 Nanocyte Inc. Sterile preparations and compositions including stinging capsules and methods of producing and using same
IL155097A0 (en) * 2000-09-28 2003-10-31 Nanocyte Inc Methods, compositions and devices utilizing stinging cells/capsules for delivering a therapeutic or a cosmetic agent into a tissue
US7632522B2 (en) * 2000-09-28 2009-12-15 Nanocyte Inc. Use of stinging cells/capsules for the delivery of active agents to keratinous substances
US8071740B2 (en) 2000-11-17 2011-12-06 Vascular Biogenics Ltd. Promoters exhibiting endothelial cell specificity and methods of using same for regulation of angiogenesis
CA2429342C (en) 2000-11-17 2013-03-19 Vascular Biogenics Ltd. Promoters exhibiting endothelial cell specificity and methods of using same
AU2002218461B2 (en) 2000-11-24 2006-09-07 Vascular Biogenics Ltd Methods employing and compositions containing defined oxidized phospholipids for prevention and treatment of atherosclerosis
JP2004524010A (ja) 2000-11-30 2004-08-12 モルキューラー スキンケア リミテッド 疾患の診断および処置
EP1337152A4 (en) * 2000-11-30 2004-08-11 Yeda Res & Dev METHODS OF USING CULTURED T CELLS THAT DO NOT INDUCE HOST Graft Reaction (GVHD) TO TREAT DISEASE
AU2002236608A1 (en) * 2000-12-14 2002-06-24 Nyxis Neurotherapies, Inc. High throughput assay to detect inhibitors of the map kinase pathway
US7087395B1 (en) 2001-01-16 2006-08-08 Quest Diagnostics Investments Incorporated Vitamin D assay
CA2440317A1 (en) * 2001-01-17 2002-07-25 Ramot At Tel Aviv University Ltd. Chitinases, derived from carnivorous plants polynucleotide sequences encoding thereof, and methods of isolating and using same
US20050202098A1 (en) 2001-01-31 2005-09-15 Tolaren Disease therapy using dying or dead cells
WO2002060376A2 (en) * 2001-01-31 2002-08-08 Tolaren Induction of tolerance by apoptotic and/or necrotic cells
US20030003454A1 (en) 2001-03-23 2003-01-02 Yeda Research And Development Co. Ltd. At The Weizmann Institute Of Science Methods and kits for determining a risk to develop cancer, for evaluating an effectiveness and dosage of cancer therapy and for correlating between an activity of a DNA repair enzyme and a cancer
US6770622B2 (en) 2001-06-08 2004-08-03 Gary A. Jarvis N-terminally truncated galectin-3 for use in treating cancer
WO2003008534A2 (en) * 2001-07-19 2003-01-30 Yissum Research Development Company Of The Hebrew University Of Jerusalem Polypeptides having carotenoids isomerase catalytic activity, nucleic acids encoding same and uses thereof
US7442781B2 (en) 2001-08-16 2008-10-28 Urifer Ltd. Diagnosis, prevention and treatment of cancer
US7138144B2 (en) * 2001-09-07 2006-11-21 Nadir Askenasy Method of inducing immune tolerance via blood/lymph flow-restricted bone marrow transplantation
US20040136972A1 (en) 2001-09-07 2004-07-15 Yeda Research And Development Co. Ltd. Methods of treating disease by transplantation of developing allogeneic or xenogeneic organs or tissues
US20060068405A1 (en) * 2004-01-27 2006-03-30 Alex Diber Methods and systems for annotating biomolecular sequences
EP2277887A3 (en) 2001-10-19 2011-02-16 Vascular Biogenics Ltd. Polynucleotide constructs, pharmaceutical compositions and methods for targeted downregulation of angiogenesis and anticancer therapy
AUPR842501A0 (en) * 2001-10-23 2001-11-15 Epipop Pty Ltd A method for identification and development of therapeutic agents
US8178128B2 (en) * 2001-12-05 2012-05-15 Yissum Research Development Company Of The Hebrew University Of Jerusalem Ltd. Nanoparticles containing polymeric nucleic acid homologs
US20050085432A1 (en) * 2001-12-26 2005-04-21 Aviva Lapidot Methods of using conjugates of saccharides and acetamidino or guanidino compounds for treating bacterial infections
IL152904A0 (en) * 2002-01-24 2003-06-24 Gamida Cell Ltd Utilization of retinoid and vitamin d receptor antagonists for expansion of renewable stem cell populations
WO2003062404A1 (en) 2002-01-25 2003-07-31 Gamida-Cell Ltd. Methods of expanding stem and progenitor cells and expanded cell populations obtained thereby
US7781396B2 (en) * 2002-01-31 2010-08-24 Tel Aviv University Future Technology Development L.P. Peptides directed for diagnosis and treatment of amyloid-associated disease
US20040052928A1 (en) * 2002-09-06 2004-03-18 Ehud Gazit Peptides and methods using same for diagnosing and treating amyloid-associated diseases
US6992176B2 (en) * 2002-02-13 2006-01-31 Technion Research & Development Foundation Ltd. Antibody having a T-cell receptor-like specificity, yet higher affinity, and the use of same in the detection and treatment of cancer, viral infection and autoimmune disease
JP2005533486A (ja) * 2002-02-20 2005-11-10 ダイアックス、コープ Ｍｈｃ−ペプチド複合体結合リガンド
US20050220774A1 (en) * 2002-03-18 2005-10-06 Tony Peled Methods of inducing differentiation in ex vivo expanded stem cells
EP1519755B1 (en) * 2002-03-26 2008-05-07 Nanocyte Inc. Stinging cells expressing an exogenous polynucleotide encoding a therapeutic, diagnostic or a cosmetic agent and methods compositions and devices utilizing such stinging cells or capsules derived therefrom for delivering the therapeutic, diagnostic or cosmetic agent into a tissue
US20040121442A1 (en) * 2002-05-05 2004-06-24 Ilan Chet Fungal chitinase, polynucleotide sequences encoding same, promoters of same, and uses thereof
AU2003242967A1 (en) * 2002-07-12 2004-02-02 Yissum Research Development Company Of The Hebrew University Of Jerusalem Method and device for inducing biological processes by micro-organs
EP1524989B1 (en) 2002-08-01 2013-11-06 Yeda Research And Development Co., Ltd. Method and composition for protecting neuronal tissue from damage induced by elevated glutamate levels
US7267981B2 (en) 2002-10-07 2007-09-11 Technion Research & Development Foundation Ltd. Human foreskin fibroblasts for culturing ES cells
AU2003284968A1 (en) * 2002-10-25 2004-05-13 University Of South Florida Methods and compounds for disruption of cd40r/cd40l signaling in the treatment of alzheimer's disease
EP2295980A1 (en) 2002-10-30 2011-03-16 Yissum Research Development Company, of The Hebrew University of Jerusalem Molecules and methods using same for measuring non-transferrin bound iron
US20050054097A1 (en) * 2002-11-17 2005-03-10 Tony Peled EX-VIVO expansion of hematopoietic system cell populations in mononuclear cell cultures
WO2004050826A2 (en) * 2002-11-29 2004-06-17 Technion Research & Development Foundation Ltd. Method of dynamically culturing embryonic stem cells
US7491699B2 (en) * 2002-12-09 2009-02-17 Ramot At Tel Aviv University Ltd. Peptide nanostructures and methods of generating and using the same
ATE486637T1 (de) 2002-12-12 2010-11-15 R B T Rakuto Bio Technologies Verwendung von ligninperoxidase zur haut- und haaraufhellung
ES2571355T3 (es) 2002-12-16 2016-05-24 Technion Res & Dev Foundation Sistema de cultivo sin células alimentadoras ni xenocontaminantes para células madre embrionarias humanas
WO2004060135A2 (en) 2003-01-02 2004-07-22 Rappaport Family Institute For Research In The Medical Sciences Methods of predicting a benefit of antioxidant therapy for prevention of cardiovascular disease in hyperglycemic patients
EP2058275A1 (en) 2003-01-07 2009-05-13 Ramot at Tel-Aviv University Ltd. Peptide nanostructures encapsulating a foreign material and method of manufacturing same
EP2319857A3 (en) 2003-03-04 2012-06-27 Yeda Research And Development Co., Ltd. Pon polypeptides, polynucleotides encoding same and compositions and methods utilizing same
EP1601759A4 (en) * 2003-03-07 2007-02-14 Gamida Cell Ltd EXPANSION OF RENEWABLE STEM CELL POPULATIONS USING PI-3 KINASE MODULATORS
US8017113B2 (en) 2003-03-12 2011-09-13 Rappaport Family Institute For Research In The Medical Sciences Compositions and methods for diagnosing and treating an inflammation
US7294701B2 (en) * 2003-04-02 2007-11-13 Technion Research & Development Foundation Ltd. Antibody fragment capable of modulating multidrug resistance and compositions and kits and methods using same
DK2330132T3 (da) 2003-04-04 2013-11-04 Yeda Res & Dev Antistoffer mod MMP2 eller MMP9 og farmaceutiske sammensætninger deraf til hæmning af deres metalloproteinaktivitet
AU2004227204B2 (en) 2003-04-08 2010-06-03 Yeda Research And Development Co. Ltd Stem cells having increased sensitivity to SDF-1 and methods of generating and using same
FR2853551B1 (fr) 2003-04-09 2006-08-04 Lab Francais Du Fractionnement Formulation stabilisante pour compositions d'immunoglobulines g sous forme liquide et sous forme lyophilisee
US7321065B2 (en) * 2003-04-18 2008-01-22 The Regents Of The University Of California Thyronamine derivatives and analogs and methods of use thereof
MXPA05012565A (es) 2003-05-22 2006-05-25 Evogene Ltd Metodos para incrementar la tolerancia al estres abiotico y/o biomasa en plantas y plantas generadas de ese modo.
AU2005234725B2 (en) 2003-05-22 2012-02-23 Evogene Ltd. Methods of Increasing Abiotic Stress Tolerance and/or Biomass in Plants and Plants Generated Thereby
US7554007B2 (en) * 2003-05-22 2009-06-30 Evogene Ltd. Methods of increasing abiotic stress tolerance and/or biomass in plants
US20050020499A1 (en) * 2003-05-27 2005-01-27 Bar Ilan University Methods of attenuating cocaine seeking behavior employing glial cell-derived neurotrophic factor (GDNF) and pharmaceutical compositions and articles of manufacture suited for use in practice of the method
WO2004111183A2 (en) * 2003-06-19 2004-12-23 Evogene Ltd. Plant trichome-specific promoter and leucoplast signal sequence
US8129514B2 (en) * 2003-06-19 2012-03-06 Evogene Ltd. Nucleotide sequences for regulating gene expression in plant trichomes and constructs and methods utilizing same
EP1638517A4 (en) 2003-06-30 2010-01-06 Univ Tel Aviv Future Tech Dev PEPTIDES, ANTIBODIES AGAINST DISEASES ASSOCIATED WITH AMYLOID AND METHODS OF USE FOR THE DIAGNOSIS AND TREATMENT OF THESE DISEASES
US20050271643A1 (en) * 2003-08-14 2005-12-08 Iryna Sorokulova Bacterial strains, compositions including same and probiotic use thereof
US7625707B2 (en) 2003-10-02 2009-12-01 Ramot At Tel Aviv University Ltd. Antibacterial agents and methods of identifying and utilizing same
SI1678209T1 (sl) 2003-10-07 2011-07-29 Yeda Res & Dev Protitelesa proti nik, njihova priprava in uporaba
IL158287A0 (en) 2003-10-07 2004-05-12 Yeda Res & Dev Antibodies to nik, their preparation and use
US20060257429A1 (en) * 2003-10-23 2006-11-16 Dreier Kimberly J Vaccine for periodontal disease
JP5039383B2 (ja) 2003-11-30 2012-10-03 イエダ リサーチ アンド ディベロップメント カンパニー リミテッド 免疫モジュレーションのための方法および薬剤ならびに免疫モジュレーターの同定方法
WO2005059100A2 (en) * 2003-12-12 2005-06-30 New York University Methods and compositions relating to cystatin c
US20100221233A1 (en) * 2003-12-15 2010-09-02 University Of South Florida Compositions and methods for enhancing neuroprotection via administration of stem cells and blood brain barrier permeabilizers
EP2289567A3 (en) 2003-12-22 2011-06-22 Regentis Biomaterials Ltd. Matrix comprising naturally-occurring crosslinked protein backbone
US8007847B2 (en) 2004-01-13 2011-08-30 Eytan Biderman Feeding formula appliance
US7423139B2 (en) * 2004-01-20 2008-09-09 Insight Biopharmaceuticals Ltd. High level expression of recombinant human erythropoietin having a modified 5′-UTR
JP2007526337A (ja) 2004-03-02 2007-09-13 アクセルロン ファーマ インコーポレーテッド Ａｌｋ７およびミオスタチン阻害剤ならびにその使用
US8352031B2 (en) 2004-03-10 2013-01-08 Impulse Dynamics Nv Protein activity modification
EP1766058A4 (en) * 2004-06-14 2008-05-21 Evogene Ltd POLYNUCLEOTIDES AND POLYPEPTIDES INVOLVED IN THE DEVELOPMENT OF PLANT FIBERS AND METHOD OF USE THEREOF
US8680062B2 (en) * 2004-07-06 2014-03-25 Deliversir Ltd. System for delivering therapeutic agents into living cells and cells nuclei
WO2006006172A2 (en) * 2004-07-15 2006-01-19 Ramot At Tel Aviv University Ltd. Use of anti-amyloid agents for treating and typing pathogen infections
WO2006030442A2 (en) 2004-09-16 2006-03-23 Gamida-Cell Ltd. Methods of ex vivo progenitor and stem cell expansion by co-culture with mesenchymal cells
US8000900B2 (en) 2004-09-21 2011-08-16 Microsoft Corporation Association-based predictions of pathogen characteristics
US20060095241A1 (en) * 2004-10-29 2006-05-04 Microsoft Corporation Systems and methods that utilize machine learning algorithms to facilitate assembly of aids vaccine cocktails
US8039240B2 (en) * 2004-09-29 2011-10-18 Yissum Reseach Development Company Of The Hebrew University Of Jerusalem Recombinant human T2 RNase and uses thereof
EP1809751B1 (en) 2004-09-29 2010-09-01 CollPlant Ltd. Collagen producing plants and methods of generating and using same
WO2006040765A1 (en) * 2004-10-12 2006-04-20 Closed Loop Therapies Ltd. Methods and implantable devices for treating supraventricular arrhythmias
US8478535B2 (en) 2004-10-29 2013-07-02 Microsoft Corporation Systems and methods that utilize machine learning algorithms to facilitate assembly of aids vaccine cocktails
US20060134680A1 (en) * 2004-12-22 2006-06-22 Alexander Kotlyar Homogeneous populations of nucleic acids, methods of synthesyzing same and uses thereof
JP2008529325A (ja) * 2005-01-16 2008-07-31 ズランゴー リミテッド 通信ネットワークシステムおよびそれを使用するための方法
WO2006075334A2 (en) 2005-01-16 2006-07-20 Zlango Ltd. Iconic communication
US8019818B2 (en) * 2005-01-18 2011-09-13 Zlango Ltd. Communications network system and methods for using same
ES2562406T3 (es) 2005-01-31 2016-03-04 Realbio Technologies Ltd. Dispositivo capilar de flujo lateral de reacción multietapa
AU2006215300C9 (en) 2005-02-17 2010-05-06 Hadasit Medical Research Services And Development Ltd. Bisphosphonates for treating endometriosis
WO2006090388A2 (en) 2005-02-25 2006-08-31 The State Of Israel, Ministry Of Agriculture & Rural Development, Agricultural Research Organization, (A.R.O.), Volcani Center Fruit cell culture extract for treating inflammation
US7695927B2 (en) * 2005-03-18 2010-04-13 Detroit R & D Detection of hypertension using glucuronidated metabolic products
WO2006109312A2 (en) * 2005-04-15 2006-10-19 Vascular Biogenics Ltd. Compositions containing beta 2-glycoprotein i-derived peptides for the prevention and/or treatment of vascular disease
US8834862B2 (en) * 2005-04-19 2014-09-16 Nanocyte Inc. Methods, compositions and devices utilizing stinging cells/capsules for conditioning a tissue prior to delivery of an active agent
ES2609919T3 (es) 2005-04-28 2017-04-25 Ventana Medical Systems, Inc. Enzimas conjugados con anticuerpos mediante un conector de PEG heterobifuncional
EP1893241A2 (en) * 2005-04-28 2008-03-05 Ventana Medical Systems, Inc. Fluorescent nanoparticles conjugated to antibodies via a peg linker
WO2006126208A2 (en) 2005-05-26 2006-11-30 Yissum Research Development Company Of The Hebrew University Of Jerusalem Compositions and methods using same for delivering agents into a target organ protected by a blood barrier
ES2524996T3 (es) 2005-06-16 2014-12-16 Ramot At Tel Aviv University Ltd. Células aisladas y poblaciones que comprenden a las mismas para el tratamiento de enfermedades del SNC
US20070225242A1 (en) * 2005-06-21 2007-09-27 The Board Of Trustees Of The Leland Stanford Junior University Method and composition for treating and preventing tumor metastasis in vivo
CA2613619A1 (en) 2005-07-07 2007-01-18 Fulcrum Sp Ltd. Sp1 polypeptides, modified sp1 polypeptides and uses thereof
WO2007007317A1 (en) * 2005-07-07 2007-01-18 Yissum Research Development Company Of The Hebrew University Of Jerusalem Nucleic acid agents for downregulating h19, and methods of using same
EP1904638A2 (en) 2005-07-18 2008-04-02 Metabogal Ltd. Mucosal or enteral administration of biologically active macromolecules
CA2616766A1 (en) 2005-07-26 2007-02-01 University Of Medicine And Dentistry Of New Jersey Antibody profiles characteristic of tuberculosis state
US8642330B2 (en) * 2005-08-08 2014-02-04 Onconon, Llc Antibody compositions, methods for treating neoplastic disease and methods for regulating fertility
US8916147B2 (en) 2005-08-24 2014-12-23 Yeda Research and Devolpment Co. Ltd. Universal donor-derived tolerogenic cells for inducing non-syngeneic transplantation tolerance
EP3354723B1 (en) 2005-08-29 2023-12-13 Technion Research & Development Foundation Ltd. Media for culturing stem cells
DE602006014009D1 (de) 2005-09-22 2010-06-10 Yissum Res Dev Co Nukleinsäure konstrukten, pharmazeutische kompositionen und methoden für die behandlung von krebs
US20070087437A1 (en) * 2005-10-14 2007-04-19 Jifan Hu Methods for rejuvenating cells in vitro and in vivo
CA2626592C (en) 2005-10-24 2018-07-17 Evogene Ltd. Methods of increasing abiotic stress tolerance and fertilizer use efficiency of a plant
CN101535244B (zh) * 2005-11-23 2014-07-23 文塔納医疗系统公司 分子缀合物
CA2632288A1 (en) 2005-11-29 2007-06-07 Gamida-Cell Ltd. Methods of improving stem cell homing and engraftment
US8846393B2 (en) 2005-11-29 2014-09-30 Gamida-Cell Ltd. Methods of improving stem cell homing and engraftment
US20070134739A1 (en) * 2005-12-12 2007-06-14 Gyros Patent Ab Microfluidic assays and microfluidic devices
WO2007069940A1 (en) 2005-12-12 2007-06-21 Gyros Patent Ab Microfluidic assays and microfluidic devices
US8775526B2 (en) 2006-01-16 2014-07-08 Zlango Ltd. Iconic communication
US8450269B2 (en) 2006-02-03 2013-05-28 Prolor Biotech Ltd. Long-acting growth hormone and methods of producing same
US7553941B2 (en) 2006-02-03 2009-06-30 Modigene Inc Long-acting polypeptides and methods of producing same
US8048849B2 (en) 2006-02-03 2011-11-01 Modigene, Inc. Long-acting polypeptides and methods of producing same
US20140113860A1 (en) 2006-02-03 2014-04-24 Prolor Biotech Ltd. Long-acting polypeptides and methods of producing and administering same
US8759292B2 (en) 2006-02-03 2014-06-24 Prolor Biotech, Llc Long-acting coagulation factors and methods of producing same
US10351615B2 (en) 2006-02-03 2019-07-16 Opko Biologics Ltd. Methods of treatment with long-acting growth hormone
US8476234B2 (en) 2006-02-03 2013-07-02 Prolor Biotech Inc. Long-acting coagulation factors and methods of producing same
US8048848B2 (en) 2006-02-03 2011-11-01 Prolor Biotech Ltd. Long-acting interferons and derivatives thereof and methods thereof
US9249407B2 (en) 2006-02-03 2016-02-02 Opko Biologics Ltd. Long-acting coagulation factors and methods of producing same
US20150038413A1 (en) 2006-02-03 2015-02-05 Opko Biologics Ltd. Long-acting polypeptides and methods of producing and administering same
US9458444B2 (en) 2006-02-03 2016-10-04 Opko Biologics Ltd. Long-acting coagulation factors and methods of producing same
US8946155B2 (en) 2006-02-03 2015-02-03 Opko Biologics Ltd. Long-acting polypeptides and methods of producing and administering same
US10221228B2 (en) 2006-02-03 2019-03-05 Opko Biologics Ltd. Long-acting polypeptides and methods of producing and administering same
CA2641079A1 (en) 2006-02-06 2007-08-16 Rappaport Family Institute For Research In The Medical Sciences Methods and kit for diagnosing t1dm
WO2007102146A2 (en) 2006-03-06 2007-09-13 Zetiq Technologies Ltd. Methods and compositions for identifying a cell phenotype
ES2572214T3 (es) 2006-03-23 2016-05-30 Pluristem Ltd Métodos para expansión celular y usos de células y medios acondicionados producidos de este modo para terapia
WO2007110869A2 (en) 2006-03-28 2007-10-04 Tel Hashomer Medical Research Infrastructure And Services Ltd. Methods and kits for determining predisposition to warfarin resistance
KR20090029184A (ko) * 2006-04-07 2009-03-20 더 가브먼트 오브 더 유나이티드 스테이츠 오브 아메리카, 리프리젠티드 바이 더 세크러테리, 디파트먼트 오브 헬쓰 앤드 휴먼 서비씨즈 항체 조성물 및 신생물성 질병의 치료 방법
CA2652538C (en) * 2006-05-19 2016-06-28 Teva Pharmaceutical Industries, Ltd. Fusion proteins, uses thereof and processes for producing same
US20070281883A1 (en) * 2006-06-05 2007-12-06 Hanna Rosenfeld Development of follicle stimulating hormone agonists and antagonists in fish
WO2008010228A2 (en) 2006-07-20 2008-01-24 Yeda Research And Development Co. Ltd. Photosyntheticorganisms and compositions and methods of generating same
US8809052B2 (en) 2006-08-28 2014-08-19 Yeda Research And Development Co. Ltd. Methods of generating mature oligodendrocytes
US7888059B2 (en) * 2006-10-05 2011-02-15 Yeda Research And Development Co. Ltd. Highly purified and stabilized Na,K-ATPase isoforms and methods of producing same
CA2664972C (en) 2006-10-05 2016-07-26 Technion Research & Development Foundation Ltd. Microtubes and methods of producing same
CA2672756C (en) * 2006-12-20 2018-11-20 Evogene Ltd. Polynucleotides and polypeptides involved in plant fiber development and methods of using same
EP2111450A2 (en) * 2007-01-16 2009-10-28 Yissum Research Development Company of the Hebrew University of Jerusalem Nucleic acid constructs and methods for specific silencing of h19
US20100196403A1 (en) * 2007-01-29 2010-08-05 Jacob Hochman Antibody conjugates for circumventing multi-drug resistance
WO2008093341A2 (en) 2007-01-31 2008-08-07 Technion Research & Development Foundation Ltd. Electrospun scaffolds and methods of generating and using same
AU2008211549B2 (en) 2007-02-01 2012-12-06 Abraham Katzir Albumin fibers and fabrics and methods of generating and using same
EP2839839B1 (en) 2007-02-28 2018-01-17 Yeda Research And Development Company Limited Nuclear targeting sequences
WO2008120202A2 (en) 2007-03-29 2008-10-09 Technion Research & Development Foundation Ltd. Antibodies, methods and kits for diagnosing and treating melanoma
KR20100015774A (ko) 2007-04-02 2010-02-12 라모트 앳 텔-아비브 유니버시티 리미티드 암세포 검출 방법 및 그 질병의 진단 및 그 질병의 치료의 모니터링을 위한 상기 방법의 용도
MX341624B (es) 2007-04-09 2016-08-26 Evogene Ltd Polinucleotidos, polipeptidos y metodos para aumentar el contenido de aceite, la velocidad de crecimiento y biomasa de las plantas.
US9156865B2 (en) 2007-04-23 2015-10-13 Deliversir Ltd System for delivering therapeutic agents into living cells and cells nuclei
PT2144633E (pt) * 2007-04-23 2014-10-27 Deliversir Ltd Um sistema para a administração de agentes terapêuticos em células vivas e núcleos de células
US9556210B2 (en) 2007-04-23 2017-01-31 Sabag-Rfa Ltd. System for delivering therapeutic agents into living cells and cells nuclei
EP3032259B1 (en) 2007-05-01 2020-04-15 Tel Hashomer Medical Research Infrastructure and Services Ltd. Methods for detecting trophoblast cells in maternal blood based on cytokeratin-7
DK2150276T3 (da) 2007-05-03 2014-08-18 Agency Science Tech & Res Antistoffer, som binder til et intracellulært prl-1- eller prl-3-polypeptid
ES2660667T3 (es) 2007-05-07 2018-03-23 Protalix Ltd. Biorreactor desechable a gran escala
US20100197767A1 (en) 2007-07-11 2010-08-05 Lior Nissim Nucleic acid construct systems capable of daignosing or treating a cell state
CA2696833A1 (en) 2007-07-15 2009-01-22 Yitzchak Hillman Disease treatment via antimicrobial peptides or their inhibitors
IL184627A0 (en) 2007-07-15 2008-12-29 Technion Res & Dev Foundation Agents for diagnosing and modulating metastasis and fibrosis as well as inflammation in a mammalian tissue
ES2547305T3 (es) 2007-07-24 2015-10-05 Evogene Ltd. Polinucleótidos, polipéptidos codificados por los mismos, y métodos de uso de los mismos para aumentar la tolerancia al estrés abiótico y/o la biomasa y/o el rendimiento en plantas que los expresan
EP2537529B1 (en) 2007-08-02 2018-10-17 Gilead Biologics, Inc. Loxl2 inhibitory antibodies and uses thereof
RU2474433C2 (ru) 2007-08-15 2013-02-10 ЙЕДА РЕСЕАРЧ ЭНД ДЕВЕЛОПМЕНТ КО. ЛТД, эт ве Вайзманн Институте оф Сайнсе Регуляторы ммр-9 и их применение
CN101855549A (zh) 2007-09-12 2010-10-06 耶达研究及发展有限公司 在免疫豁免部位内治疗肿瘤的方法
KR20140107677A (ko) 2007-09-19 2014-09-04 플루리스템 리미티드 지방 또는 태반 조직 유래의 부착 세포 및 이의 치료 용도
ES2620487T3 (es) 2007-10-19 2017-06-28 Rappaport Family Institute For Research In The Medical Sciences Composiciones que comprenden semaforinas para uso en el tratamiento del cáncer
EP2220112B1 (en) 2007-11-26 2015-08-26 Yissum Research Development Company of the Hebrew University of Jerusalem Ltd. Compositions comprising fibrous polypeptides and polysaccharides
MX357387B (es) 2007-12-27 2018-07-06 Evogene Ltd Polipeptidos, polinucleotidos aislados utiles para modificar la eficacia en el uso de agua, eficacia en el uso del fertilizante, la tolerancia al estres biotico/abiotico, el rendimiento y biomasa en plantas.
US20110052501A1 (en) * 2008-01-31 2011-03-03 Liat Dassa Polypeptides and polynucleotides, and uses thereof as a drug target for producing drugs and biologics
WO2009104174A2 (en) 2008-02-21 2009-08-27 Technion Research & Development Foundation Ltd . A method of attaching a cell-of-interest to a microtube
EP2297200A1 (en) * 2008-04-09 2011-03-23 Technion Research & Development Foundation Ltd. Anti influenza antibodies and uses thereof
WO2009125394A1 (en) * 2008-04-09 2009-10-15 Technion Research & Development Foundation Ltd. Anti human immunodeficiency antibodies and uses thereof
WO2009125381A1 (en) * 2008-04-10 2009-10-15 Objet Geometries Ltd. System and method for three dimensional model printing
US9095569B2 (en) 2008-04-18 2015-08-04 Collplant Ltd. Methods of generating and using procollagen
US8791324B2 (en) 2008-04-21 2014-07-29 Danziger Innovations Ltd. Plant viral expression vectors and use of same for generating genotypic variations in plant genomes
EP2116618A1 (en) 2008-05-09 2009-11-11 Agency for Science, Technology And Research Diagnosis and treatment of Kawasaki disease
MX2010012697A (es) 2008-05-22 2011-03-15 Evogene Ltd Polinucleotidos y polipeptidos aislados y metodos para usarlos para incrementar el rendimiento de plantas, biomasa, velocidad de crecimiento, vigor, contenido de aceite, tolerancia al estres abiotico de las plantas y eficiencia de uso de nitrogeno.
RU2515156C2 (ru) 2008-05-27 2014-05-10 Плуристем Лтд. Способы лечения воспалительных заболеваний ободочной кишки
DK2620493T3 (da) 2008-05-28 2019-07-01 Univ Ramot Mesenkymale stamceller til behandling af CNS-sygdomme
US9952211B2 (en) 2008-06-29 2018-04-24 Realbio Technologies Ltd. Liquid-transfer device particularly useful as a capturing device in a biological assay process
EP2331107A4 (en) 2008-08-08 2011-08-24 Agency Science Tech & Res VHZ FOR DIAGNOSIS AND TREATMENT OF CANCERS
AU2009279458B2 (en) 2008-08-08 2015-07-02 Code Biotherapeutics, Inc. Long-acting DNA dendrimers and methods thereof
MX2011001741A (es) 2008-08-18 2011-03-29 Evogene Ltd Polipeptidos y polinucleotidos aislados utiles para mejorar la eficacia en el uso de nitrogeno tolerancia al estres abiotico, rendimiento y biomasa en plantas.
CN102282249A (zh) 2008-09-02 2011-12-14 普拉里斯坦有限公司 来自胎盘组织的粘附细胞及其在治疗中的用途
US8367392B2 (en) * 2008-09-05 2013-02-05 Transalgae Ltd. Genetic transformation of algal and cyanobacteria cells by microporation
EP2326340A4 (en) 2008-09-11 2013-01-23 Univ Ben Gurion COMPOSITIONS AND METHODS FOR TREATING PNEUMOCOCCAL INFECTIONS
CN102186970A (zh) 2008-09-12 2011-09-14 克里奥普拉斯低温生物有限公司 缺血性组织的细胞疗法
CN104892728A (zh) 2008-09-24 2015-09-09 特尔哈肖梅尔医学研究基础设施和服务有限公司 用于防止细胞粘附的肽和组合物及其使用方法
EP2342356A4 (en) 2008-09-29 2012-11-21 Univ Ben Gurion AMYLOID BETA PEPTIDASES AND METHOD OF USE THEREOF
BRPI0914522B1 (pt) 2008-10-30 2019-04-02 Evogene Ltd Método para aumentar o rendimento, a biomassa, a taxa de crescimento, o vigor, oteor de óleo, e/ou a eficiência da utilização do nitrogênio de uma planta
US20110229892A1 (en) 2008-11-17 2011-09-22 Yehuda G Assaraf Method for predicting a patient's responsiveness to anti-folate therapy
WO2010058396A1 (en) 2008-11-19 2010-05-27 Yissum Research Development Company Of The Hebrew University Of Jerusalem Ltd. A cd44vra antibody and diagnostic and therapeutic methods using same
WO2010061383A1 (en) 2008-11-26 2010-06-03 Biodalia Microbiological Technologies Ltd. A method of in-situ enrichment of foods with fructan
WO2010064231A1 (en) 2008-12-02 2010-06-10 Tel Hashomer Medical Research Infrastructure And Services Ltd. Methods of analyzing a-i rna editing and nucleic acid constructs capable of same
US20120014927A1 (en) 2008-12-03 2012-01-19 Tel Hashomer Medical Research Infrastructure And Services Ltd. Methods and kits for determining predisposition to cancer
US8734759B2 (en) 2008-12-05 2014-05-27 Yeda Research And Development Co. Ltd. Methods of diagnosing and treating motor neuron diseases
WO2010071610A1 (en) 2008-12-19 2010-06-24 Agency For Science, Technology And Research (A*Star) Severe chikungunya biomarkers
CA2744827C (en) 2008-12-29 2019-10-15 Evogene Ltd. Polynucleotides, polypeptides encoded thereby, and methods of using same for increasing abiotic stress tolerance, biomass and/or yield in plants expressing same
CA2967412C (en) 2008-12-29 2018-07-17 Tel Hashomer Medical Research, Infrastructure And Services Ltd. Peptides and compositions for prevention of cell adhesion and methods of using same
WO2010076788A2 (en) 2008-12-29 2010-07-08 Yissum Research Development Company Of The Hebrew University Of Jerusalem Ltd. Methods of predicting responsiveness to interferon treatment and treating hepatitis c infection
WO2010076794A1 (en) 2008-12-31 2010-07-08 Technion Research & Development Foundation Ltd. Method of denitrifying brine and systems capable of same
US9107935B2 (en) 2009-01-06 2015-08-18 Gilead Biologics, Inc. Chemotherapeutic methods and compositions
IL196820A0 (en) 2009-02-01 2009-11-18 Yissum Res Dev Co Devitalized, acellular scaffold matrices derived from micro-organs seeded with various cells
WO2010086867A2 (en) 2009-02-02 2010-08-05 Ramot At Tel Aviv University Ltd. Peptides, pharmaceutical compositions comprising same and uses thereof
WO2010089707A1 (en) 2009-02-04 2010-08-12 Yeda Research And Development Co. Ltd. Methods and kits for determining sensitivity or resistance of prostate cancer to radiation therapy
EP2465920B1 (en) 2009-02-26 2017-04-12 Tel HaShomer Medical Research Infrastructure and Services Ltd. Isolated populations of renal stem cells and methods of isolating and using same
AR075773A1 (es) 2009-03-02 2011-04-27 Evogene Ltd Polipetidos y polinucleotidos aislados y metodos para su uso en el aumento del rendimiento de plantas y las caracteristicas agricolas
PL2406279T3 (pl) 2009-03-09 2016-07-29 Univ Ramot Kompozycje do zapobiegania i leczenia chorób neurodegeneratywnych.
WO2010103517A1 (en) 2009-03-12 2010-09-16 Rappaport Family Institute For Research In The Medical Sciences Soluble compositions for the treatment of cxcr3-ligand associated diseases
US20120020954A1 (en) 2009-03-30 2012-01-26 Anat Achiron Methods of predicting clinical course and treating multiple sclerosis
AU2010231514A1 (en) 2009-04-01 2011-11-03 Rappaport Family Institute For Research In The Medical Sciences A method of regulating proliferation and differentiation of keratinocytes
WO2010116375A1 (en) 2009-04-08 2010-10-14 Yeda Research And Development Co. Ltd. Isolated peptides for regulating apoptosis
EP2990421B1 (en) 2009-04-30 2018-02-21 Tel HaShomer Medical Research Infrastructure and Services Ltd. Anti ceacam1 antibodies and methods of using same
WO2010134073A1 (en) 2009-05-19 2010-11-25 Zetiq Technologies Ltd. Kits for and methods of differential staining of cervical cancer cells and/or tissues
US20120083477A1 (en) 2009-05-27 2012-04-05 Yeda Research And Development Co. Ltd. Proteasome inhibitors and uses thereof
US20120078163A1 (en) 2009-05-27 2012-03-29 Yissum Research Development Company Of The Hebrew University Of Jerusalem Ltd. Method of generating connective tissue
WO2010137013A1 (en) 2009-05-27 2010-12-02 Ramot At Tel Aviv University Ltd. Crystallized photosystem i units from the pea plant and their use in solid state devices
EP2435078A1 (en) 2009-05-28 2012-04-04 Yeda Research and Development Co. Ltd. Methods of treating inflammation
MX363447B (es) 2009-06-10 2019-03-22 Evogene Ltd Polinucleotidos y polipeptidos aislados, y metodos para utilizarlos para mejorar la eficacia en el uso de nitrogeno, rendimiento, tasa de crecimiento, vigor, biomasa, contenido de aceite y/o tolerancia al estres abiotico.
WO2010150259A1 (en) 2009-06-24 2010-12-29 Fund For Medical Research Development Of Infrastructure And Health Services -Rambam Medical Center Methods and kits for isolating placental derived microparticles and use of same for diagnosis of fetal disorders
US9663778B2 (en) 2009-07-09 2017-05-30 OPKO Biologies Ltd. Long-acting coagulation factors and methods of producing same
WO2011004379A1 (en) 2009-07-10 2011-01-13 Tel Hashomer Medical Research Infrastructure And Services Ltd. Compositions and methods for treating cancer
CA2768725A1 (en) 2009-07-21 2011-01-27 Tel Hashomer Medical Research Infrastructure And Services Ltd. A method of diagnosing cancer
GB0913442D0 (en) 2009-07-31 2009-09-16 Univ Ramot Cell-targeting nanoparticles comprising polynucleotide agents and uses thereof
WO2011021194A2 (en) 2009-08-17 2011-02-24 Technion Research & Development Foundation Ltd. Pericyte progenitor cells and methods of generating and using same
CA2771774A1 (en) * 2009-08-21 2011-02-24 Gilead Biologics, Inc. In vitro screening assays
WO2011021171A1 (en) 2009-08-21 2011-02-24 Beeologics, Llc Preventing and curing beneficial insect diseases via plant transcribed molecules
AU2010284036B2 (en) 2009-08-21 2014-12-18 Gilead Biologics, Inc. Catalytic domains from lysyl oxidase and LOXL2
CN106619515A (zh) 2009-08-27 2017-05-10 工业研究与发展基金会有限公司 脂质体组合物及其用途
US9610331B2 (en) 2009-09-08 2017-04-04 Yeda Research And Development Co. Ltd. Methods for hematopoietic precursor mobilization
WO2011030336A1 (en) 2009-09-08 2011-03-17 Ramot At Tel-Aviv University Ltd. Methods of diagnosing amyotrophic lateral sclerosis (als)
US20120171213A1 (en) 2009-09-10 2012-07-05 Yissum Research Development Company Of The Hebrew University Of Jerusalem Ltd. Method of treating tumors
US8735124B2 (en) 2009-09-17 2014-05-27 Yeda Research And Development Co. Ltd. Isolated PON1 polypeptides, polynucleotides encoding same and uses thereof in treating or preventing organophosphate exposure associated damage
WO2011033506A2 (en) 2009-09-17 2011-03-24 Yeda Research And Development Co. Ltd. Isolated pon1 polypeptides, polynucleotides encoding same and uses thereof in treating or preventing organophosphate exposure associated damage
CA2773935C (en) 2009-09-17 2018-06-19 Ramot At Tel Aviv University Ltd. Peptides for the treatment of oxidative stress related disorders
US20110081706A1 (en) * 2009-10-02 2011-04-07 TransAlgae Ltd Method and system for efficient harvesting of microalgae and cyanobacteria
US8962584B2 (en) 2009-10-14 2015-02-24 Yissum Research Development Company Of The Hebrew University Of Jerusalem, Ltd. Compositions for controlling Varroa mites in bees
CN102906263B (zh) 2009-10-14 2017-05-31 耶路撒冷希伯来大学伊森姆研究发展公司 用于控制蜂中的瓦螨的组合物
WO2011046570A1 (en) 2009-10-16 2011-04-21 The University Of Medicine And Dentistry Of New Jersey Method for treating chronic nerve tissue injury using a cell therapy strategy
WO2011048600A1 (en) 2009-10-21 2011-04-28 Danziger Innovations Ltd. Generating genotypic variations in plant genomes by gamete infection
IL201999A (en) 2009-11-08 2017-10-31 Medical Res & Development Fund For Health Services Bnai Zion Medical Center The State Of Israel A knockout mouse of the 1 – mo gene, a gene associated with morbidly extreme obesity
EP2498806B1 (en) 2009-11-12 2014-12-31 Ramot at Tel-Aviv University Ltd. Compositions comprising pedf and uses of same in the treatment and prevention of ovary-related syndromes
EP4166652A1 (en) 2009-11-12 2023-04-19 Technion Research & Development Foundation Ltd. Culture media, cell cultures and methods of culturing pluripotent stem cells in an undifferentiated state
WO2011058555A1 (en) 2009-11-12 2011-05-19 Yeda Research And Development Co. Ltd. A method of editing dna in a cell and constructs capable of same
US20120230974A1 (en) 2009-11-17 2012-09-13 Protalix Ltd Alkaline alpha galactosidase for the treatment of fabry disease
WO2011064773A1 (en) 2009-11-24 2011-06-03 Collplant Ltd. Method of generating collagen fibers
EP2806023B1 (en) 2009-11-30 2015-09-23 Pluristem Ltd. Adherent cells from placenta and use of same in disease treatment
WO2011067745A2 (en) 2009-12-06 2011-06-09 Rosetta Green Ltd. Compositions and methods for enhancing plants resistance to abiotic stress
EP2510098B1 (en) 2009-12-09 2015-02-11 Quark Pharmaceuticals, Inc. Methods and compositions for treating diseases, disorders or injury of the cns
US9493785B2 (en) 2009-12-28 2016-11-15 Evogene Ltd. Isolated polynucleotides and polypeptides and methods of using same for increasing plant yield, biomass, growth rate, vigor, oil content, abiotic stress tolerance of plants and nitrogen use efficiency
EP3785712A1 (en) 2009-12-29 2021-03-03 Gamida-Cell Ltd. Methods for enhancing natural killer cell proliferation and activity
CA2786374C (en) 2010-01-05 2019-01-22 Vascular Biogenics Ltd. Methods for use of a specific anti-angiogenic adenoviral agent
EP2523681A1 (en) 2010-01-12 2012-11-21 Vascular Biogenics Ltd. Methods of producing adenovirus vectors and viral preparations generated thereby
US8697078B2 (en) 2010-01-27 2014-04-15 Yeda Research And Development Co. Ltd. Antibodies that inhibit metalloproteins
CN103370080A (zh) 2010-02-04 2013-10-23 吉联亚生物科技有限公司 结合赖氨酰氧化酶样2（loxl2）的抗体和其使用方法
WO2011099006A2 (en) 2010-02-11 2011-08-18 Yeda Research And Development Co. Ltd. Enzymatic systems for carbon fixation and methods of generating same
WO2011104708A2 (en) 2010-02-24 2011-09-01 Ben Gurion University Of The Negev Research And Development Authority Methods for inhibiting necrosis
WO2011107939A1 (en) 2010-03-01 2011-09-09 Yissum Research Development Company Of The Hebrew University Of Jerusalem Ltd. Methods of predicting efficacy of an anti-vegfa treatment for solid tumors
WO2011107994A1 (en) 2010-03-04 2011-09-09 Yeda Research And Development Co. Ltd. Methods of measuring protein stability
US9267142B2 (en) 2010-03-08 2016-02-23 Yeda Research And Development Co. Ltd. Recombinant protein production in heterologous systems
MX2012010479A (es) 2010-03-08 2012-10-09 Monsanto Technology Llc Moleculas polinucleotidicas para regulacion genetica en plantas.
WO2011111050A2 (en) 2010-03-11 2011-09-15 Jacob Edrei Methods of generating hydrogen
US9073990B2 (en) 2010-04-05 2015-07-07 Bar-Llan University Protease-activatable pore-forming polypeptides
US8927274B2 (en) 2010-04-12 2015-01-06 Technion Research & Development Foundation Limited Populations of pancreatic progenitor cells and methods of isolating and using same
WO2011128895A2 (en) 2010-04-12 2011-10-20 Ben Gurion University Of The Negev Research And Development Authority Sulfotransferase of a red microalga and uses thereof
CN103109185A (zh) 2010-04-18 2013-05-15 耶达研究及发展有限公司 用于治疗ErbB/ErbB配体相关疾病的分子和使用所述分子的方法
BR122021002340B1 (pt) 2010-04-28 2022-04-05 Evogene Ltd Método de aumento de biomassa, taxa de crescimento e/ou eficiência de uso de nitrogênio de uma planta
US20130053277A1 (en) 2010-05-04 2013-02-28 Yissum Research Development Company Of The Hebrew University Of Jerusalem Ltd. Methods of identifying inhibitors of polypeptides-of-interest
WO2011138785A2 (en) 2010-05-05 2011-11-10 Rappaport Family Institute For Research In The Medical Sciences Use of ccl1 in therapy
IL208820A0 (en) 2010-10-19 2011-01-31 Rachel Teitelbaum Biologic female contraceptives
EP2569417B1 (en) 2010-05-13 2018-04-18 Tel HaShomer Medical Research Infrastructure and Services Ltd. Isolated populations of adult renal cells and methods of isolating and using same
EP2390664B1 (de) 2010-05-25 2013-04-17 Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. Verfahren zur elektrochemischen Detektion von Bindungsreaktionen
US8945943B2 (en) 2010-05-26 2015-02-03 The Board Of Trustees Of The University Of Illinois Personal glucose meters for detection and quantification of a broad range of analytes
EP2575859B1 (en) 2010-06-03 2018-09-19 Ramot at Tel Aviv University, Ltd. Methods of treating diabetes and compositions capable of same
US20130316351A1 (en) 2010-06-07 2013-11-28 Osnat Ashur-Fabian Methods and kits for diagnosing conditions related to hypoxia
WO2011158243A2 (en) 2010-06-16 2011-12-22 Yissum Research Development Company Of The Hebrew University Of Jerusalem Ltd. Method of diagnosing and treating cancer
BR112012032126A2 (pt) 2010-06-16 2017-10-17 Futuragene Israel Ltd Empresa Isralense polinucleotídeo isolado, estrutura de ácido nucléico, sistema de estrutura de ácido nucléico, polipeptídeo isolado, planta, composição inseticida, e método para controlar ou exterminar um inseto
ES2817780T3 (es) 2010-07-12 2021-04-08 The State Of Israel Ministry Of Agriculture And Rural Development Agricultural Res Organization A R Polinucleótidos aislados y métodos y plantas que usan los mismos para regular la acidez de las plantas
JP2013538555A (ja) 2010-07-15 2013-10-17 テクニオン リサーチ アンド ディベロップメント ファウンデーション リミテッド Ｍｈｃクラスｉｉとグルタミン酸デカルボキシラーゼ（ｇａｄ）自己抗原性ペプチドとの天然型複合体に対するｔ細胞受容体様特異性を有する単離された高親和性実体
CN103108955B (zh) 2010-07-15 2015-11-25 工业研究与发展基金会有限公司 用于提高植物中非生物胁迫耐受性的核酸构建体
JP2013535963A (ja) 2010-07-15 2013-09-19 テクニオン リサーチ アンド ディベロップメント ファウンデーション リミテッド Ｍｈｃクラスｉｉと糖尿病関連自己抗原性ペプチドとの天然型複合体に対するｔ細胞受容体様特異性を有する単離された高親和性実体
US9624469B2 (en) 2010-07-22 2017-04-18 Cellect Biotherapeutics Ltd. Regulatory immune cells with enhanced targeted cell death effect
WO2012014208A2 (en) 2010-07-27 2012-02-02 Yeda Research And Development Co. Ltd. Methods and systems for assessing clonality of cell cultures
WO2012014207A2 (en) 2010-07-27 2012-02-02 Technion Research & Development Foundation Ltd. Method for generating induced pluripotent stem cells from keratinocytes derived from plucked hair follicles
WO2012014205A1 (en) 2010-07-27 2012-02-02 Technion Research & Development Foundation Ltd. Isolated mesenchymal progenitor cells and extracellular matrix produced thereby
WO2012017439A2 (en) 2010-08-04 2012-02-09 Ramot At Tel-Aviv University Ltd. Methods of treating autoimmune diseases of the central nervous system (cns) and neurodegenerative diseases
EP2606122A1 (en) 2010-08-22 2013-06-26 Ramot at Tel Aviv University, Ltd. Induced pluripotent stem cells derived from human pancreatic beta cells
WO2012025925A1 (en) 2010-08-24 2012-03-01 Rappaport Family Institute For Research In The Medical Sciences Methods of improving transplantation using sdf-1alpha
MX365161B (es) 2010-08-30 2019-05-24 Evogene Ltd Polinucleótidos y polipéptidos aislados, y métodos para utilizarlos para aumentar la eficacia en el uso de nitrógeno, rendimiento, tasa de crecimiento, vigor, biomasa, contenido de aceite y/o tolerancia al estrés abiótico.
WO2012032519A2 (en) 2010-09-07 2012-03-15 Yissum Research Development Company Of The Hebrew University Of Jerusalem Ltd. Methods of diagnosing parkinson's disease
WO2012032520A1 (en) 2010-09-07 2012-03-15 Yissum Research Development Company Of The Hebrew University Of Jerusalem Ltd. Readthrough acetylcholinesterase (ache-r) for treating or preventing parkinson's disease
WO2012032510A1 (en) 2010-09-07 2012-03-15 Yeda Research And Development Co. Ltd. Primers for amplifying dna and methods of selecting same
CA2810488A1 (en) 2010-09-07 2012-03-15 Technion Research & Development Foundation Limited Novel methods and culture media for culturing pluripotent stem cells
CN103282047B (zh) 2010-09-08 2016-08-24 耶达研究及发展有限公司 抗第三方中枢记忆性t细胞在抗白血病/淋巴瘤治疗中的用途
CA2810631A1 (en) 2010-09-08 2012-03-15 Yeda Research And Development Co. Ltd. An immunosuppressive drug combination for a stable and long term engraftment
CN103210082A (zh) 2010-09-15 2013-07-17 雷蒙特亚特特拉维夫大学有限公司 扩增胰岛β细胞和使其再分化的方法
WO2012038956A1 (en) 2010-09-20 2012-03-29 Yissum Research Development Company Of The Hebrew University Of Jerusalem Ltd. Method of treating neurodegenerative diseases
WO2012052872A2 (en) 2010-10-17 2012-04-26 Yeda Research And Development Co. Ltd. Methods and compositions for the treatment of insulin-associated medical conditions
WO2012052878A1 (en) 2010-10-19 2012-04-26 Vascular Biogenics Ltd. Isolated polynucleotides and nucleic acid constructs for directing expression of a gene-of-interest in cells
WO2012056452A2 (en) 2010-10-27 2012-05-03 Nanocyte (Israel) Ltd. Pharmaceutical compositions and delivery devices comprising stinging cells or capsules
WO2012056455A1 (en) 2010-10-28 2012-05-03 Yeda Research And Development Co. Ltd. Methods of generating antibodies to metalloenzymes
AR083708A1 (es) 2010-11-03 2013-03-13 Yissum Res Dev Co Plantas transgenicas con rendimientos de sacarificacion altos y metodos para generarlas
EP2635105B1 (en) 2010-11-04 2017-12-20 Ben-Gurion University of The Negev Research and Development Authority Acyl-coa: diacylglycerol acyltransferase 1-like gene (ptdgat1) and uses thereof
TW201225972A (en) 2010-11-15 2012-07-01 Univ Ramot Dipeptide analogs for treating conditions associated with amyloid fibril formation
EP2640404B1 (en) 2010-11-17 2017-02-22 Ben Gurion University of The Negev Research and Development Authority T-cell therapy to neurodegenerative diseases
US9574171B2 (en) 2010-12-02 2017-02-21 Technion Research & Development Foundation Ltd. Methods of generating corneal cells and cell populations comprising same
AU2011253984A1 (en) 2010-12-07 2012-06-28 Biobalance Llc Method For Identifying E. Coli M-17
CA2816495C (en) 2010-12-15 2020-10-13 Kadimastem Ltd. Insulin producing cells derived from pluripotent stem cells
US9551006B2 (en) 2010-12-22 2017-01-24 Evogene Ltd. Isolated polynucleotides and polypeptides, and methods of using same for improving plant properties
AU2011350620B2 (en) 2010-12-28 2017-03-02 Kamedis Ltd. Plant extracts for the treatment and prevention of infections
DK2665814T3 (en) 2011-01-20 2017-09-11 Protalix Ltd NUCLEIC ACID CONSTRUCTION FOR EXPRESSION OF ALPHA-GALACTOSIDASE IN PLANTS AND PLANT CELLS
US20130316358A1 (en) 2011-01-31 2013-11-28 Yeda Research And Development Co. Ltd. Methods of diagnosing disease using overlap extension pcr
US20130330349A1 (en) 2011-02-23 2013-12-12 Rappaport Family Institute For Research In The Medical Sciences High affinity molecules capable of binding a type a plexin receptor and uses of same
UA113843C2 (xx) 2011-03-02 2017-03-27 Стійка до бактерій трансгенна рослина, яка містить дисфункціональні білки t3ss
EP2680864B1 (en) 2011-03-03 2019-05-08 Ramot at Tel-Aviv University Ltd. Genetically modified muscle cells which express neurotrophic factors
ES2687771T3 (es) 2011-03-06 2018-10-29 Merck Serono Sa Líneas de células con bajo nivel de fucosa y sus usos
US9061006B2 (en) 2011-03-08 2015-06-23 Ramot At Tel-Aviv University Ltd. Compositions and methods for diagnosing and treating phenylketonuria (PKU)
WO2012123938A1 (en) 2011-03-17 2012-09-20 Tel Hashomer Medical Research Infrastructure And Services Ltd. Quinolone analogs for treating autoimmune diseases
WO2012123949A1 (en) 2011-03-17 2012-09-20 Ramot At Tel-Aviv University Ltd. Bi- and monospecific, asymmetric antibodies and methods of generating the same
JP6030114B2 (ja) 2011-03-22 2016-11-24 プルリステム リミテッド 放射線照射または化学物質による傷害を治療するための方法
KR20140036164A (ko) 2011-03-24 2014-03-25 뉴림 파머슈티칼스 (1991) 리미티드 신경보호 펩타이드
WO2012131680A2 (en) 2011-03-28 2012-10-04 Yeda Research And Development Co. Ltd. Compositions and methods for treating inflammation
WO2012137202A1 (en) 2011-04-05 2012-10-11 Yissum Research Development Company Of The Hebrew University Of Jerusalem Ltd. Composition comprising an iron indicator attached to a microparticle and uses of same for quantifying non-transferrin bound iron (ntbi) and cellular labile iron (lci)
ES2627154T3 (es) 2011-04-06 2017-07-26 Ramot At Tel-Aviv University Ltd. Métodos de seguimiento y análisis de perfiles de actividad metabólica, usos diagnósticos y terapéuticos de los mismos
US9150644B2 (en) 2011-04-12 2015-10-06 The United States Of America, As Represented By The Secretary, Department Of Health And Human Services Human monoclonal antibodies that bind insulin-like growth factor (IGF) I and II
CN103476924A (zh) 2011-04-15 2013-12-25 普拉里斯坦有限公司 收获细胞的方法和系统
CA3137663A1 (en) 2011-05-03 2012-11-08 Evogene Ltd. Isolated polynucleotides and polypeptides and methods of using same for increasing plant yield, biomass, growth rate, vigor, oil content, abiotic stress tolerance of plants and nitrogen use efficiency
US9192670B2 (en) 2011-05-04 2015-11-24 Ramot At Tel-Aviv University Ltd. Regulation of amyloid beta molecular composition for the treatment of alzheimer's disease
WO2012153333A1 (en) 2011-05-09 2012-11-15 Yissum Research Development Company Of The Hebrew University Of Jerusalem Ltd. Regeneration and repair of mesenchymal tissue using amelogenin
WO2012153336A2 (en) 2011-05-12 2012-11-15 Rakuto Bio Technologies Ltd. Methods and device for lightening skin complexion
WO2012156976A1 (en) 2011-05-16 2012-11-22 Yissum Research Development Company Of The Hebrew University Of Jerusalem Ltd. Methods of producing artemisinin in non-host plants and vectors for use in same
US20140088017A1 (en) 2011-05-23 2014-03-27 Yeda Research And Development Co., Ltd. Use of akt phosphorylation as a biomarker for prognosing neurodegenerative diseases and treating same
WO2012160526A2 (en) 2011-05-23 2012-11-29 Ofir Menashe Formulations of microorganism comprising particles and uses of same
EP2714719B1 (en) 2011-05-31 2018-12-12 Hutchison Biofilm Medical Solutions Limited Dispersion and detachment of cell aggregates
WO2012164565A1 (en) 2011-06-01 2012-12-06 Yeda Research And Development Co. Ltd. Compositions and methods for downregulating prokaryotic genes
US10166295B2 (en) 2011-06-02 2019-01-01 Opko Biologics Ltd. Pegylated OXM variants
US9415103B2 (en) 2011-06-14 2016-08-16 Yeda Research And Development Co. Ltd. Combination therapy to prevent DCIS formation and progression to breast cancer
MX2014000180A (es) 2011-06-23 2014-03-27 Kaiima Bio Agritech Ltd Trigo comun, sus plantas o partes que tienen un genoma parcial o completamente multiplicado, sus hibridos y productos y metodos para generarlos y utilizarlos.
CN103929950A (zh) 2011-07-20 2014-07-16 凯伊玛生物农业科技有限公司 具有部分或完全倍增基因组的玉米植物及其用途
EP2550982A1 (en) 2011-07-27 2013-01-30 Technion Research & Development Foundation Ltd. Devices for surgical applications
EP3524679B1 (en) 2011-08-04 2021-10-06 Yeda Research and Development Co., Ltd. Micro-rna mir-19 and compositions comprising same for the treatment of medical conditions in which low adrenaline or noradrenaline level is therapeutically beneficial
JP6063613B2 (ja) 2011-08-09 2017-01-18 イェダ リサーチ アンド デベロップメント カンパニー リミテッド ベータ細胞の分化およびインスリン産生を促進するためのｍｉＲ−７のダウンレギュレーション
AU2012296138A1 (en) 2011-08-14 2014-03-06 Kaiima Bio Agritech Ltd. Durum wheat plants having a partially or fully multiplied genome and uses thereof
EP2753367A4 (en) 2011-09-07 2015-10-14 Yeda Res & Dev OLFACTORY SIGNATURE AND ODORING MIXTURE THEREWITH
WO2013035071A1 (en) 2011-09-08 2013-03-14 Yeda Research And Development Co. Ltd. Novel risk biomarkers for lung cancer
CN103930130B (zh) 2011-09-08 2016-07-06 耶达研究及发展有限公司 抗第三方中央型记忆t细胞、其产生方法以及其在移植和疾病治疗中的应用
US10760086B2 (en) 2011-09-13 2020-09-01 Monsanto Technology Llc Methods and compositions for weed control
US10806146B2 (en) 2011-09-13 2020-10-20 Monsanto Technology Llc Methods and compositions for weed control
EP2755988B1 (en) 2011-09-13 2018-08-22 Monsanto Technology LLC Methods and compositions for weed control
US10829828B2 (en) 2011-09-13 2020-11-10 Monsanto Technology Llc Methods and compositions for weed control
EP3296402B1 (en) 2011-09-13 2020-04-15 Monsanto Technology LLC Methods and compositions for weed control
CA2848680C (en) 2011-09-13 2020-05-19 Monsanto Technology Llc Methods and compositions for weed control
EP2755466A4 (en) 2011-09-13 2015-04-15 Monsanto Technology Llc METHODS AND COMPOSITIONS FOR CONTROLLING WEEDS
WO2013054320A1 (en) 2011-10-11 2013-04-18 Tel Hashomer Medical Research Infrastructure And Services Ltd. Antibodies to carcinoembryonic antigen-related cell adhesion molecule (ceacam)
EP2768851B1 (en) 2011-10-21 2017-05-31 Augurex Life Sciences Corporation Antigens derived from citrullinated 14-3-3 and uses thereof in the diagnosis of rheumatoid arthritis
WO2013061328A2 (en) 2011-10-27 2013-05-02 Yeda Research And Development Co. Ltd. Method of treating cancer
WO2013067076A2 (en) 2011-11-03 2013-05-10 Quark Pharmaceuticals, Inc. Methods and compositions for neuroprotection
EP2776565A1 (en) 2011-11-08 2014-09-17 Quark Pharmaceuticals, Inc. Methods and compositions for treating diseases, disorders or injury of the nervous system
US9736998B2 (en) 2011-11-23 2017-08-22 Danziger Dan Flower Farm Otomeria plants
US9617331B2 (en) 2011-11-27 2017-04-11 Yeda Research And Development Co. Ltd. Methods of regulating angiogenesis by administering agents which increase apoB-100 polypeptide
JP2015500279A (ja) 2011-12-08 2015-01-05 イェダ リサーチ アンド デベロップメント カンパニー リミテッド 哺乳動物胎児肺細胞および該細胞の治療的使用
WO2013093921A1 (en) 2011-12-20 2013-06-27 Collplant Ltd. Collagen coated synthetic polymer fibers
WO2013095729A1 (en) 2011-12-22 2013-06-27 Life Technologies Corporation Sequential lateral flow capillary device for analyte determination
CN104470542A (zh) 2011-12-22 2015-03-25 耶达研究及发展有限公司 用于稳定和长期植入的联合疗法
WO2013098820A1 (en) 2011-12-28 2013-07-04 Kaiima Bio Agritech Ltd. Cultivated sorghum plant having a partially or fully multiplied genome and uses of same
JP2015509085A (ja) 2012-01-01 2015-03-26 キュービーアイ エンタープライゼズ リミテッドＱｂｉ Ｅｎｔｅｒｐｒｉｓｅｓ Ｌｔｄ． 治療剤および診断剤の選択的送達のためのｅｎｄｏ１８０を標的とする粒子
WO2013114363A2 (en) 2012-01-30 2013-08-08 Yeda Research And Development Co.Ltd. Antimicrobial agents
US9603907B2 (en) 2012-02-01 2017-03-28 Protalix Ltd. Dry powder formulations of dNase I
WO2013118120A2 (en) 2012-02-06 2013-08-15 Rosetta Green Ltd. Isolated polynucleotides expressing or modulating micrornas or targets of same, transgenic plants comprising same and uses thereof in improving nitrogen use efficiency, abiotic stress tolerance, biomass, vigor or yield of a plant
SG11201404608WA (en) 2012-02-13 2014-09-26 Gamida Cell Ltd Mesenchymal stem cells conditioned medium and methods of generating and using the same
KR102310704B1 (ko) 2012-02-14 2021-10-08 옵코 바이오로직스 리미티드 장기 작용 응고 인자들 및 이를 생산하는 방법들
AU2013220005A1 (en) 2012-02-19 2014-09-04 Protalix Ltd. Oral unit dosage forms and uses of same for the treatment of Gaucher disease
JP6329911B2 (ja) 2012-02-22 2018-05-23 ブレインステム バイオテック リミテッド 星状膠細胞作製のためのミクロｒｎａ
JP2015509366A (ja) 2012-02-22 2015-03-30 ブレインステム バイオテック リミテッド 神経幹細胞および運動ニューロンの生成
EP2833714A1 (en) 2012-03-01 2015-02-11 The State of Israel - Ministry of Agriculture & Rural Development, Agricultural Research Organization (ARO) (Volcani Center) Male sterile garlic plants, hybrid offspring of same and methods of generating and using same
EP3483184A1 (en) 2012-03-07 2019-05-15 Yeda Research and Development Co. Ltd Compositions for inhibition of quiescinsulfhydryl oxidase (qsox1) and uses of same
US9334308B2 (en) 2012-03-22 2016-05-10 Ramot At Tel-Aviv University Ltd. PLIF multimeric peptides and uses thereof
WO2013156989A1 (en) 2012-04-18 2013-10-24 Ramot At Tel-Aviv University Ltd. Lipidated glycosaminoglycan particles for the delivery of nucleic acids
CN104487082A (zh) 2012-04-19 2015-04-01 奥普科生物制品有限公司 长效胃泌酸调节素变体及其生产方法
EP2841084B1 (en) 2012-04-24 2018-05-30 Biokine Therapeutics Ltd. Cxcr4 antagonist peptide for use in the treatment of large cell lung cancer
EP2846819B1 (en) 2012-05-09 2017-05-03 Yeda Research and Development Co. Ltd. Variants of tace pro-domain as tnf-a inhibitor and their medical use
US8865158B2 (en) 2012-05-22 2014-10-21 Ramot At Tel-Aviv University Ltd. Bacteriophages for reducing toxicity of bacteria
US10240161B2 (en) 2012-05-24 2019-03-26 A.B. Seeds Ltd. Compositions and methods for silencing gene expression
JP5868549B2 (ja) 2012-05-24 2016-02-24 マウントゲイト グループ リミテッド 狂犬病感染の予防および治療に関する組成物および方法
EP2855507B1 (en) 2012-06-03 2018-01-03 Ben-Gurion University of The Negev Research and Development Authority Functionalized titanium binding peptides and implants coated with same
SG10201702228QA (en) 2012-06-04 2017-04-27 Opko Biolog Ltd Pegylated oxm variants
WO2014006621A1 (en) 2012-07-03 2014-01-09 Nanospun Technologies Ltd. Methods and devices for adsorption and biodegradation of petroleum
US9175266B2 (en) 2012-07-23 2015-11-03 Gamida Cell Ltd. Enhancement of natural killer (NK) cell proliferation and activity
US9567569B2 (en) 2012-07-23 2017-02-14 Gamida Cell Ltd. Methods of culturing and expanding mesenchymal stem cells
WO2014020599A1 (en) 2012-07-29 2014-02-06 Yeda Research And Development Co. Ltd. Use of the reductive glycine pathway for generating formatotrophic and autotrophic microorganisms
JP6340366B2 (ja) 2012-07-31 2018-06-06 イェダ リサーチ アンド デベロップメント カンパニー リミテッド 運動ニューロン疾患を診断および処置する方法
ES2813407T3 (es) 2012-08-06 2021-03-23 Brainstorm Cell Therapeutics Ltd Métodos para generar células madre mesenquimales que secretan factores neurotróficos
WO2014033723A1 (en) 2012-09-03 2014-03-06 A.B. Seeds Ltd. Method of improving abiotic stress tolerance of plants and plants generated thereby
US10543278B2 (en) 2012-09-12 2020-01-28 Ramot At Tel-Aviv University Ltd. Immunoparticles and methods of generating and using same
WO2014057490A1 (en) 2012-10-09 2014-04-17 Ramot At Tel-Aviv University Ltd. Methods and kits for predicting prognosis of cancer using soluble mortalin in blood
CN104918639B (zh) 2012-10-22 2018-01-26 萨拜格Rfa公司 用于将治疗剂递送到活细胞和细胞核中的系统
EP2912062A1 (en) 2012-10-24 2015-09-02 Yeda Research and Development Co. Ltd. Combinations of epidermal growth factor receptor targeting antibodies for treating cancer
EP2912181B1 (en) 2012-10-29 2017-11-29 Yeda Research and Development Co., Ltd. Aptamers, multimeric aptamers and uses thereof
BR122020018510B1 (pt) 2012-11-20 2023-03-14 Opko Biologics Ltd Método para aumentar incrementalmente o tamanho hidrodinâmico de um fator de coagulação ativado viia
WO2015079413A2 (en) 2013-11-28 2015-06-04 Yeda Research And Development Co. Ltd. Synaptojanin-2 inhibitors and uses thereof
KR20150090116A (ko) 2012-11-29 2015-08-05 예다 리서치 앤드 디벨럽먼트 캄파니 리미티드 종양 전이를 예방하고, 암을 치료하고 예후를 예측하며, 추정상의 전이 억제인자 물질을 확인하는 방법
US10683505B2 (en) 2013-01-01 2020-06-16 Monsanto Technology Llc Methods of introducing dsRNA to plant seeds for modulating gene expression
MX2015008611A (es) 2013-01-01 2016-02-03 Seeds Ltd Ab Metodos para introducir dsrna en semillas de plantas para modular la expresion genetica.
WO2014108850A2 (en) 2013-01-09 2014-07-17 Yeda Research And Development Co. Ltd. High throughput transcriptome analysis
WO2014108854A1 (en) 2013-01-09 2014-07-17 Fusimab Ltd. Monospecific anti-hgf and anti-ang2 antibodies and bispecific anti-hgf/anti-ang2 antibodies
CA2894472C (en) 2013-01-17 2021-08-17 Novellusdx Ltd. Methods and systems for identifying patient specific driver mutations
WO2014118785A1 (en) 2013-02-04 2014-08-07 Ramot At Tel-Aviv University Ltd. Generation of cytotoxic tumor specific cell lines and uses thereof
CA2902298A1 (en) 2013-03-06 2014-09-12 Protalix Ltd. Chimeric polypeptides, polynucleotides encoding same, cells expressing same and methods of producing same
CN105121460A (zh) 2013-03-06 2015-12-02 波塔力克斯有限公司 表达TNFα多肽抑制的植物细胞在治疗中的用途
EP2967082A4 (en) 2013-03-13 2016-11-02 Monsanto Technology Llc METHOD AND COMPOSITIONS FOR WEED CONTROL
BR112015023051A2 (pt) 2013-03-13 2017-11-14 Monsanto Technology Llc método para controle de ervas daninhas, composição herbicida, cassete de expressão microbiano e método de produção de polinucleotídeo
US10568328B2 (en) 2013-03-15 2020-02-25 Monsanto Technology Llc Methods and compositions for weed control
EP2976097B1 (en) 2013-03-21 2021-05-26 CollPlant Ltd. Compositions comprising collagen and prp for tissue regeneration and their production method
US20160082071A1 (en) 2013-03-24 2016-03-24 Biokine Therapeutics Ltd. Methods of treating myeloid leukemia
WO2014174511A1 (en) 2013-04-21 2014-10-30 Yeda Research And Development Co. Ltd. Agents for downregulation of the activity and/or amount of bcl-xl and/or bcl-w
CN105531365A (zh) 2013-04-23 2016-04-27 耶达研究及发展有限公司 分离的幼稚多能干细胞和产生所述细胞的方法
WO2014174520A1 (en) 2013-04-25 2014-10-30 Yeda Research And Development Co. Ltd. Use of inhibitory peptides for the treatment of inflammatory diseases
US20160115231A1 (en) 2013-05-21 2016-04-28 Yissum Research Development Company Of The Hebrew University Of Jerusalem Ltd. Treatment of mast cell related pathologies
DK3003493T3 (da) 2013-05-27 2020-01-13 Rakuto Bio Tech Ltd Enzymatisk system, som indeholder kosmetiske sammensætninger
ES2938044T3 (es) 2013-06-24 2023-04-04 Univ Ramot Estructura a base de epiplón y sistema de suministro
JP6668236B2 (ja) 2013-07-19 2020-03-18 モンサント テクノロジー エルエルシー Ｌｅｐｔｉｎｏｔａｒｓａ防除用組成物及びその方法
US9850496B2 (en) 2013-07-19 2017-12-26 Monsanto Technology Llc Compositions and methods for controlling Leptinotarsa
WO2015015489A1 (en) 2013-07-30 2015-02-05 Biolinerx Ltd. Antibody for treating diabetes and autoimmune diseases
EP3039030B1 (en) 2013-08-29 2018-09-19 Yeda Research and Development Co. Ltd. Selective inhibitors of alpha2 isoform of na,k-atpase and use for reduction of intra-ocular pressure
IL228284A (en) 2013-09-03 2016-12-29 Technion Res & Dev Foundation Implants from cellular scaffolding and blood vessel stalk
WO2015033343A1 (en) 2013-09-03 2015-03-12 Yissum Research Development Company Of The Hebrew University Of Jerusalem Ltd. Compositions and methods for expressing recombinant polypeptides
WO2015033344A1 (en) 2013-09-05 2015-03-12 Yissum Research Development Company Of The Hebrew University Of Jerusalem Ltd. Methods and kits for inhibiting pathogenicity of group a streptococcus (gas) or group g streptococcus (ggs)
WO2015037009A1 (en) 2013-09-16 2015-03-19 Plexicure Ltd. Isolated proteins capable of binding plexin-a4 and methods of producing and using same
EP3046929B1 (en) 2013-09-17 2019-01-16 Yeda Research and Development Co., Ltd. Erk-derived peptides and uses thereof
ES2796853T3 (es) 2013-10-01 2020-11-30 Kadimastem Ltd Diferenciación dirigida de astrocitos a partir de células madre pluripotentes humanas para uso en el cribado de medicamentos y el tratamiento de la esclerosis lateral amiotrófica (ELA)
DK3060239T3 (da) 2013-10-21 2020-08-03 Opko Biologics Ltd Langtidsvirkende polypeptider og fremgangsmåder til fremstilling og indgivelse heraf
US20150158926A1 (en) 2013-10-21 2015-06-11 Opko Biologics, Ltd. Long-acting polypeptides and methods of producing and administering same
US10767156B2 (en) 2013-10-24 2020-09-08 Yeda Research And Development Co., Ltd. Polynucleotides encoding BREX system polypeptides and methods of using same
CA2928315C (en) 2013-10-31 2023-03-21 Biokine Therapeutics Ltd. Methods of treating acute myeloid leukemia with a flt3 mutation
MX2016005778A (es) 2013-11-04 2016-12-20 Monsanto Technology Llc Composiciones y metodos para controlar infestaciones de plagas y parasitos de los artropodos.
ES2754207T3 (es) 2013-11-28 2020-04-16 Tel Hashomer Medical Res Infrastructure & Services Ltd Inhibidores de la ARN polimerasa I y usos de los mismos
ES2697757T3 (es) 2013-12-10 2019-01-28 Yeda Res & Dev Uso de enzimas que catalizan la síntesis de piruvato a partir de formiato y acetil-CoA y bacterias que expresan la misma
UA119253C2 (uk) 2013-12-10 2019-05-27 Біолоджикс, Інк. Спосіб боротьби із вірусом у кліща varroa та у бджіл
AR099092A1 (es) 2014-01-15 2016-06-29 Monsanto Technology Llc Métodos y composiciones para el control de malezas utilizando polinucleótidos epsps
WO2015114633A1 (en) 2014-01-30 2015-08-06 Yissum Research And Development Company Of The Hebrew University Of Jerusalem Ltd. Actin binding peptides and compositions comprising same for inhibiting angiogenes is and treating medical conditions associated with same
JP2017506259A (ja) 2014-02-03 2017-03-02 イッスム・リサーチ・ディベロップメント・カンパニー・オブ・ザ・ヘブルー・ユニバーシティ・オブ・エルサレム・リミテッドＹｉｓｓｕｍ Ｒｅｓｅａｒｃｈ Ｄｅｖｅｌｏｐｍｅｎｔ Ｃｏｍｐａｎｙ Ｏｆ Ｔｈｅ Ｈｅｂｒｅｗ Ｕｎｉｖｅｒｓｉｔｙ Ｏｆ Ｊｅｒｕｓａｌｅｍ Ｌｔｄ． 幹細胞を枯渇させるためのカゼインキナーゼｉ阻害剤の使用
JP2017506228A (ja) 2014-02-05 2017-03-02 イェダ リサーチ アンド ディベロップメント カンパニー リミテッドＹｅｄａ Ｒｅｓｅａｒｃｈ Ａｎｄ Ｄｅｖｅｌｏｐｍｅｎｔ Ｃｏ．Ｌｔｄ． セロトニン放出ホルモン、アドレナリン放出ホルモン、ノルアドレナリン放出ホルモン、グルタミン酸放出ホルモンおよび副腎皮質刺激ホルモン放出ホルモン関連の医学的状態の処置および診断のためのマイクロｒｎａおよび該マイクロｒｎａを含む組成物
WO2015118538A1 (en) 2014-02-06 2015-08-13 Yeda Research And Development Co. Ltd. Anti cd84 antibodies, compositions comprising same and uses thereof
WO2015118547A1 (en) 2014-02-10 2015-08-13 Protalix Ltd. Method of maintaining disease stability in a subject having gaucher's disease
JP6628253B2 (ja) 2014-02-11 2020-01-08 ブレインストーム セル セラペウティクス リミテッド 細胞の適性を判定する方法
US20170067894A1 (en) 2014-03-03 2017-03-09 Yissum Research Development Company Of The Hebrew University Of Jerusalem Ltd. Method and device for detection of pseudomonas aeruginosa
EP2923709A1 (en) 2014-03-28 2015-09-30 Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. Multi-component-multistage malaria vaccine
EP3125676A4 (en) 2014-04-01 2018-02-14 Monsanto Technology LLC Compositions and methods for controlling insect pests
WO2015155777A1 (en) 2014-04-10 2015-10-15 Bonus Therapeutics Ltd. Bone repair compositions
PT4026917T (pt) 2014-04-14 2024-02-12 Yissum Research And Development Company Of The Hebrew Univ Of Jerusalem Ltd Método e kit para determinar a morte de células ou de tecido ou a origem de tecidos ou de células de dna por análise de metilação do dna
ES2764504T3 (es) 2014-04-17 2020-06-03 Yeda Res & Dev Métodos y equipos para analizar restos de unión al ADN fijados al ADN
US11427647B2 (en) 2014-04-27 2022-08-30 Famewave Ltd. Polynucleotides encoding humanized antibodies against CEACAM1
MX366359B (es) 2014-04-27 2019-07-05 Ccam Biotherapeutics Ltd Anticuerpos humanizados contra la molecula de adhesion celular relacionada al antigeno carcinoembrionico 1 (ceacam1).
EP3058085B1 (en) 2014-04-28 2021-02-17 Yeda Research and Development Co., Ltd. Microbiome response to agents
AU2015257287A1 (en) 2014-05-04 2016-11-10 Forrest Innovations Ltd. Compositions and methods for reducing pathogen-induced citrus greening
US10294482B2 (en) 2014-05-22 2019-05-21 Yeda Research And Development Co. Ltd. Recombinant microorganisms capable of carbon fixation
WO2015186128A1 (en) 2014-06-02 2015-12-10 Kadimastem Ltd. Methods of inducing myelination and maturation of oligodendrocytes
US20170253881A1 (en) 2014-06-02 2017-09-07 Technion Research & Development Foundation Limited Compositions and methods of selectively inhibiting irp1 and treating inflammation
CA2953347A1 (en) 2014-06-23 2015-12-30 Monsanto Technology Llc Compositions and methods for regulating gene expression via rna interference
EP3145951A1 (en) 2014-06-24 2017-03-29 InSight Biopharmaceuticals Ltd. Methods of purifying antibodies
ES2791199T3 (es) 2014-06-25 2020-11-03 Tel Hashomer Medical Res Infrastructure & Services Ltd Identificación de marcadores de células madre cancerosas y uso de los mismos para diagnóstico y tratamiento
US11807857B2 (en) 2014-06-25 2023-11-07 Monsanto Technology Llc Methods and compositions for delivering nucleic acids to plant cells and regulating gene expression
EP3160448A4 (en) 2014-06-26 2018-11-14 Ramot at Tel-Aviv University Ltd. Liposomal formulations for delivery of nucleic acids
WO2016005985A2 (en) 2014-07-09 2016-01-14 Yissum Research Development Company Of The Hebrew University Of Jerusalem Ltd. Method for reprogramming cells
JP6671363B2 (ja) 2014-07-15 2020-03-25 イッサム リサーチ ディヴェロップメント カンパニー オブ ザ ヘブリュー ユニバーシティー オブ エルサレム リミティッド Ｃｄ４４の単離されたポリペプチドおよびその使用
EP3172562B1 (en) 2014-07-21 2019-03-13 Novellusdx Ltd. Methods and systems for determining oncogenic index of patient specific mutations
JP6700249B2 (ja) 2014-07-21 2020-06-10 ノベルラスディクス リミテッド 患者特有の変異の薬物応答を測定する方法
CN114009454A (zh) 2014-07-29 2022-02-08 孟山都技术公司 用于控制昆虫害虫的组合物和方法
EP3194572B1 (en) 2014-07-30 2023-10-25 Yeda Research and Development Co. Ltd. Media for culturing pluripotent stem cells
US10303846B2 (en) 2014-08-14 2019-05-28 Memed Diagnostics Ltd. Computational analysis of biological data using manifold and a hyperplane
WO2016030899A1 (en) 2014-08-28 2016-03-03 Yeda Research And Development Co. Ltd. Methods of treating amyotrophic lateral scleroses
EP2992895A1 (en) 2014-09-08 2016-03-09 Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. Three-component-multistage malaria vaccine
US10526416B2 (en) 2014-09-08 2020-01-07 Yeda Research And Development Co. Ltd. Anti-HER3 antibodies and uses of same
WO2016038610A1 (en) 2014-09-08 2016-03-17 Yeda Research And Development Co. Ltd. Compositions and methods for treating cancer resistant to a tyrosine kinase inhibitor (tki)
IL234638A0 (en) 2014-09-14 2014-12-02 Yeda Res & Dev NMDA receptor antagonists for the treatment of Gaucher disease
WO2016042561A2 (en) 2014-09-21 2016-03-24 Yissum Research Development Company Of The Hebrew University Of Jerusalem Ltd. Downregulating mir-132 for the treatment of lipid related disorders
GB2536745B (en) 2014-10-20 2019-06-26 Gen Probe Inc Red Blood cell lysis solution
CN107406825B (zh) 2014-11-13 2022-04-08 恩祖迪克控股有限公司 甲壳纲十足目雌性动物的功能性别反转
US20180042994A1 (en) 2014-11-17 2018-02-15 Yeda Research And Development Co. Ltd. Methods of treating diseases related to mitochondrial function
CN107429291A (zh) 2014-11-17 2017-12-01 耶达研究及发展有限公司 分析微生物组的方法
EP3221455B1 (en) 2014-11-20 2020-08-12 Yissum Research and Development Company of the Hebrew University of Jerusalem Ltd. Compositions and methods for producing polypeptides with a modified glycosylation pattern in plant cells
CN107208070B (zh) 2014-11-26 2021-09-07 技术创新动力基金（以色列）有限合伙公司 细菌基因的靶向消除
KR20170081268A (ko) 2014-11-27 2017-07-11 단지거 이노베이션즈 엘티디. 게놈 편집용 핵산 구조체
WO2016092555A2 (en) 2014-12-11 2016-06-16 Yeda Research And Development Co. Ltd. Isolated phosphotriesterase polypeptides, polynucleotides encoding same and uses thereof in treating or preventing organophosphate exposure associated damage
WO2016103269A1 (en) 2014-12-23 2016-06-30 Ramot At Tel-Aviv University Ltd. Populations of neural progenitor cells and methods of producing and using same
CN114469998A (zh) 2014-12-30 2022-05-13 细胞治疗神经科学有限公司 治疗视网膜疾病的方法
JP6882981B2 (ja) 2014-12-30 2021-06-02 セル キュア ニューロサイエンシズ リミテッド Ｒｐｅ細胞集団およびそれを作製する方法
WO2016108240A1 (en) 2014-12-30 2016-07-07 Cell Cure Neurosciences Ltd. Assessing retinal pigment epithelial cell populations
US11225648B2 (en) 2015-01-04 2022-01-18 Protalix Ltd. Modified DNase and uses thereof
WO2016116935A1 (en) 2015-01-21 2016-07-28 Yeda Research And Development Co. Ltd. Use of rasa2 as a prognostic and therapeutic marker for melanoma
CN108064288B (zh) 2015-01-22 2021-11-26 孟山都技术公司 用于控制叶甲属的组合物和方法
US20180345047A1 (en) 2015-02-26 2018-12-06 Yeda Research And Development Co. Ltd. Method of promoting hair growth
EP3268475B1 (en) 2015-03-11 2020-10-21 Yissum Research and Development Company of the Hebrew University of Jerusalem Ltd. Decoy oligonucleotides for the treatment of diseases
IL237852A0 (en) 2015-03-19 2016-03-24 Yeda Res & Dev Antibodies against amphigoline, medical preparations containing them and their use
JP2018518152A (ja) 2015-03-27 2018-07-12 ユニバーシティ オブ サザン カリフォルニア 充実性腫瘍を処置するためのｌｈｒに指向されたｃａｒ ｔ細胞治療
US20180064748A1 (en) 2015-03-27 2018-03-08 Yeda Research And Development Co. Ltd. Methods of treating motor neuron diseases
RU2017135552A (ru) 2015-03-31 2019-04-30 Иеда Рисеч Энд Девелопмент Ко. Лтд. Глутамат оксалоацетат трансаминаза 1 (got1), препараты и способы их получения и применения
WO2016157192A1 (en) 2015-04-01 2016-10-06 The State Of Israel, Ministry Of Agriculture & Rural Development, Agricultural Research Organization (Aro) (Volcani Center) Erodium crassifolium l'her plant extracts and uses thereof
EP3280401B1 (en) 2015-04-07 2021-08-25 ELA Pharma Ltd Compositions for treating and/or preventing cell or tissue necrosis specifically targeting cathepsin c and/or cela1 and/or cela3a and/or structurally related enzymes thereto
EP3081575A1 (en) 2015-04-12 2016-10-19 Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. Anti-plasmodium parasite antibodies
EP3288547B1 (en) 2015-04-27 2023-06-07 The Medical Research, Infrastructure, And Health Services Fund Of The Tel Aviv Medical Center Egr1 targeting molecules for the treatment of inflammatory and hyperproliferative conditions
AU2016255724B2 (en) 2015-04-29 2020-05-14 The State of Israel, Ministry of Agriculture and Rural Development Agricultural Research Organization (ARO) (Volcani Center) Anti-phytopathogenic compositions
WO2016174652A1 (en) 2015-04-30 2016-11-03 Technion Research & Development Foundation Limited Chimeric antigen receptors and methods of their use
CN108026566A (zh) 2015-05-04 2018-05-11 特拉维夫大学拉莫特有限公司 用于使dna片段化的方法和试剂盒
US20180161300A1 (en) 2015-05-11 2018-06-14 Yeda Research And Development Co., Ltd. Citrin inhibitors for the treatment of cancer
EP3298141A4 (en) 2015-05-17 2019-01-09 The Medical Research, Infrastructure, And Health Services Fund Of The Tel Aviv Medical Center COMPOSITIONS AND METHOD FOR THE TREATMENT OF CANCER
WO2016185457A1 (en) 2015-05-19 2016-11-24 Yeda Research And Development Co. Ltd. Methods of promoting lymphangiogenesis
WO2016185481A2 (en) 2015-05-20 2016-11-24 Yeda Research And Development Co. Ltd. Method of targeting senescent cells
EA201792547A1 (ru) 2015-05-21 2018-04-30 Иеда Рисеч Энд Девелопмент Ко. Лтд. Популяции бактерий для стимуляции состояния здоровья
AU2016273045B2 (en) 2015-05-29 2020-11-26 Opko Biologics Ltd. Pegylated oxyntomodulin variants
EP3302053B1 (en) 2015-06-02 2021-03-17 Monsanto Technology LLC Compositions and methods for delivery of a polynucleotide into a plant
US10655136B2 (en) 2015-06-03 2020-05-19 Monsanto Technology Llc Methods and compositions for introducing nucleic acids into plants
WO2016197064A1 (en) 2015-06-04 2016-12-08 Epstein Alan L Lym-1 and lym-2 targeted car cell immunotherapy
NL2014935B1 (en) 2015-06-08 2017-02-03 Applied Immune Tech Ltd T cell receptor like antibodies having fine specificity.
EP4026563A1 (en) 2015-06-18 2022-07-13 Yeda Research and Development Co. Ltd Conditioning protocols and use of same for tissue regeneration
WO2016203476A1 (en) 2015-06-18 2016-12-22 Yissum Research Development Company Of The Hebrew University Of Jerusalem Ltd. Methods and compositions for diagnosing and treating urothelial cancer
WO2016203482A2 (en) 2015-06-19 2016-12-22 Opko Biologics Ltd. Long-acting coagulation factors and methods of producing same
JP2018521983A (ja) 2015-07-16 2018-08-09 バイオカイン セラピューティックス リミテッド がんを治療するための組成物および方法
CA2991690A1 (en) 2015-07-16 2017-01-19 Yeda Research And Development Co. Ltd. Genetically modified anti-third party central memory t cells and use of same in immunotherapy
US10668094B2 (en) 2015-07-19 2020-06-02 Yeda Research And Development Co. Ltd. Selective inhibitors of Alpha2-containing isoforms of Na,K-ATPase and use thereof for reduction of intraocular pressure
EP3324754B1 (en) 2015-07-22 2022-09-07 Phytopharma International Ltd. Bee-ingestible compositions, methods of using same for producing honey and honey produced thereby
US11230696B2 (en) 2015-07-29 2022-01-25 Hadasit Medical Research Services And Development Ltd. Large scale production of retinal pigment epithelial cells
US20180221393A1 (en) 2015-08-03 2018-08-09 Biokine Therapeutics Ltd. Cxcr4 binding agents for treatment of diseases
WO2017021961A1 (en) 2015-08-04 2017-02-09 Yeda Research And Development Co. Ltd. Methods of screening for riboswitches and attenuators
EA201890442A1 (ru) 2015-08-05 2018-07-31 Селл Кьюр Нейросайансес Лтд. Получение фоторецепторов для лечения заболеваний сетчатки
AU2016303632B2 (en) 2015-08-05 2022-09-29 Cell Cure Neurosciences Ltd. Preparation of retinal pigment epithelium cells
WO2017025963A1 (en) 2015-08-10 2017-02-16 The Medical Research, Infrastructure and Health Services Fund of the Tel Aviv Medical Center Methods and pharmaceutical compositions for improving wound healing using cd24
US20180237790A1 (en) 2015-08-13 2018-08-23 Forrest Innovation Ltd. Formulations and compositions for delivery of nucleic acids to plant cells
WO2017033188A1 (en) 2015-08-24 2017-03-02 Yeda Research And Development Co. Ltd. Algal oil and biofuel and methods of producing same
US10765706B2 (en) 2015-09-03 2020-09-08 University Of South Florida Method of stem cell delivery into the brain during chronic disease using blood brain barrier permeabilizers
WO2017042814A1 (en) 2015-09-10 2017-03-16 Yeda Research And Development Co. Ltd. Use of perforin positive immature dendritic cells in disease treatment
EP3365376A1 (en) 2015-10-25 2018-08-29 Yeda Research and Development Co., Ltd. Antibodies targeting quiescin sulfhydryl oxidase (qsox1) and uses of same
EP3368661A1 (en) 2015-10-26 2018-09-05 Cell Cure Neurosciences Ltd. Preparation of retinal pigment epithelium cells
CN109310648B (zh) 2015-10-27 2022-06-03 杰伊制药公司 用于治疗癌症的包含大麻二酚和第二治疗剂的组合物
IL242380A0 (en) 2015-10-29 2016-02-01 Yeda Res & Dev A method for inducing the division of cardiomyocytes and treating heart diseases
WO2017077539A1 (en) 2015-11-03 2017-05-11 Ariel-University Research And Development Company Ltd. Compositions for regeneration and repair of neural tissue
WO2017081689A1 (en) 2015-11-11 2017-05-18 Ramot At Tel-Aviv University Ltd. Methods of detecting 5-hydroxymethylcytosine and diagnosing of cancer
EP3384286B1 (en) 2015-11-30 2023-04-19 Kadimastem Ltd. Methods for differentiating and purifying pancreatic endocrine cells
EP3176183A1 (en) 2015-12-02 2017-06-07 Yeda Research and Development Co. Ltd Compositions and methods for treating cancer not resistant to a tyrosine kinase inhibitor (tki)
WO2017103930A1 (en) 2015-12-16 2017-06-22 Ramot At Tel-Aviv University Ltd. Particles comprising decellularized omentum
WO2017115367A1 (en) 2015-12-28 2017-07-06 The National Institute for Biotechnology in the Negev Ltd. Composition and method for treating amyotrophic lateral sclerosis
WO2017118985A1 (en) 2016-01-06 2017-07-13 Yeda Research And Development Co. Ltd. Compositions and methods for treating malignant, autoimmune and inflammatory diseases
US20190178888A1 (en) 2016-01-11 2019-06-13 Technion Research & Development Foundation Limited Methods of determining prognosis of sepsis and treating same
JP7019580B2 (ja) 2016-01-21 2022-02-15 ザ ステイト オブ イスラエル ミニストリー オブ アグリカルチャー アンド ルーラル ディベロップメント アグリカルチュラル リサーチ オーガニゼイション （エー．アール．オー．） （ボルカニ センター） 単為結実植物およびその製造方法
IL243838A (en) 2016-01-28 2017-07-31 Sp Nano Ltd The composition containing sp1 protein and carbon nanoparticles and its uses
IL243839B (en) 2016-01-28 2018-01-31 Sp Nano Ltd Conductive wires
WO2017130205A1 (en) 2016-01-31 2017-08-03 Hadasit Medical Research Services And Development Ltd. Autosomal-identical pluripotent stem cell populations having non-identical sex chromosomal composition and uses thereof
JP7072508B2 (ja) 2016-02-04 2022-05-20 イェダ リサーチ アンド ディベロップメント カンパニー リミテッド 変異型ｐ５３と関連している疾患、障害または病状の処置におけるペプチドおよびその使用
US20190142875A1 (en) 2016-02-14 2019-05-16 Yeda Research And Development Co. Ltd. Microbiome-based diagnosis, prediction and treatment of relapsing obesity
WO2017138008A2 (en) 2016-02-14 2017-08-17 Yeda Research And Development Co. Ltd. Methods of modulating protein exocytosis and uses of same in therapy
BR112018016757A2 (pt) 2016-02-16 2020-05-19 Yissum Research Development Company Of The Hebrew University Of Jerusalem Ltd. análogos de fenilalanina não-proteícos para inibir crescimento de cianobacteria e de plantas.
US11053476B2 (en) 2016-02-23 2021-07-06 Exostem Biotec Ltd. Generation of cancer stem cells and use thereof
BR112018067522A2 (pt) 2016-03-01 2019-02-05 Univ Of Rijeka Faculty Of Medicine anticorpos específicos para receptor de poliovírus humano (pvr)
IL287757B (en) 2016-03-03 2022-07-01 Memed Diagnostics Ltd An RNA test to diagnose the type of infection
WO2017153982A1 (en) 2016-03-06 2017-09-14 Yeda Research And Development Co. Ltd. Method for modulating myelination
IL244649A0 (en) 2016-03-17 2016-06-30 Yeda Res & Dev Methods for the isolation of barrel-like proteases and the identification of peptides that are cleaved by Alidan
US10421785B2 (en) 2016-04-11 2019-09-24 Bar-Ilan University Delta receptor agonist peptides and use thereof
CA3232826A1 (en) 2016-04-27 2017-11-02 Gen-Probe Incorporated Blood cell lysis reagent
WO2017203520A1 (en) 2016-05-22 2017-11-30 Yeda Research And Development Co. Ltd. Methods of using pulmonary cells for transplantation and induction of tolerance
IL245861A0 (en) 2016-05-25 2016-09-04 Yeda Res & Dev Use of substances to treat drug-resistant tumors
US20190100732A1 (en) 2016-06-02 2019-04-04 Yissum Research Development Company Of The Hebrew University Of Jerusalem Ltd. Assay for the removal of methyl-cytosine residues from dna
US11820833B2 (en) 2016-06-05 2023-11-21 Tel Hashomer Medical Research Infrastructure And Services Ltd. Peptides that inhibit binding of EPCR to its ligand to treat inflammation
WO2017212494A1 (en) 2016-06-09 2017-12-14 Opko Biologics Ltd. Long-acting oxyntomodulin formulation and methods of producing and administering same
EP3472342A1 (en) 2016-06-19 2019-04-24 Yissum Research Development Company of the Hebrew University of Jerusalem Ltd. Screening for chemotherapy resistance in human haploid cells
AU2017289879B2 (en) 2016-06-27 2023-04-20 Yeda Research And Development Co. Ltd. Veto cells generated from memory T cells
EP3482200B1 (en) 2016-07-10 2022-05-04 Memed Diagnostics Ltd. Protein signatures for distinguishing between bacterial and viral infections
CN109804245B (zh) 2016-07-10 2022-10-25 米密德诊断学有限公司 感染的早期诊断
US20200080050A1 (en) 2016-07-11 2020-03-12 Yaakov Nahmias Systems and methods for growing cells in vitro
KR20190038833A (ko) 2016-07-11 2019-04-09 보누스 테라퓨틱스 리미티드 조직 재생용 세포 조성물
IL246722A0 (en) 2016-07-11 2016-09-29 Yeda Res & Dev Combined treatment to increase endogenous synthesis of nitric oxide
HUE064463T2 (hu) 2016-07-11 2024-03-28 Opko Biologics Ltd Hosszantartó hatású VII. koagulációs faktor és az elõállítására vonatkozó eljárások
WO2018020489A1 (en) 2016-07-24 2018-02-01 Yeda Research And Development Co. Ltd. Methods and kits for analyzing dna binding moieties attached to dna
US10982252B2 (en) 2016-08-08 2021-04-20 Technology Innovation Momentum Fund (Israel) Limited Partnership Bacterial systems for analyzing ubiquitylated polypeptides
WO2018029693A1 (en) 2016-08-10 2018-02-15 Ramot At Tel-Aviv University Ltd. Analysis of methylation status and copy number
US20190180846A1 (en) 2016-08-10 2019-06-13 Memed Diagnostics Ltd. System and method for analysis of biological data
CN109843305B (zh) 2016-08-14 2023-03-10 雷蒙特亚特特拉维夫大学有限公司 治疗神经障碍的间充质细胞来源的外泌体
IL247368A0 (en) 2016-08-18 2016-11-30 Yeda Res & Dev Diagnostic and therapeutic uses of exosomes
WO2018037416A1 (en) 2016-08-25 2018-03-01 Yeda Research And Development Co. Ltd. Methods and compositions for treating autoimmune diseases
WO2018047154A1 (en) 2016-09-07 2018-03-15 Yissum Research Development Company Of The Hebrew University Of Jerusalem Ltd. Anti-nkp46 antibodies and therapeutic use of same
WO2018051347A1 (en) 2016-09-14 2018-03-22 Yeda Research And Development Co. Ltd. Crisp-seq, an integrated method for massively parallel single cell rna-seq and crispr pooled screens
EP3519833A4 (en) 2016-09-29 2020-06-03 MeMed Diagnostics Ltd. PROGNOSTIC AND TREATMENT METHODS
IL248468A0 (en) 2016-10-13 2017-01-31 Technion Res & Dev Foundation Use of caspase-3 inhibitors and caspase-3 activators in the preparation of medical preparations for cancer treatment and wound healing
IL248385A0 (en) 2016-10-18 2017-02-01 Yeda Res & Dev Treatment of circadian rhythm disorder
US20190359956A1 (en) 2016-11-10 2019-11-28 Yeda Research And Development Co. Ltd. Phosphotriesterases for treating or preventing organophosphate exposure associated damage
US11542508B2 (en) 2016-11-28 2023-01-03 Yeda Research And Development Co. Ltd. Isolated polynucleotides and polypeptides and methods of using same for expressing an expression product of interest
EP3548015A1 (en) 2016-11-30 2019-10-09 Yeda Research and Development Co. Ltd. Methods of treating liver toxicity and disorders
MX2018016003A (es) 2016-12-01 2019-05-16 Univ Ramot Tratamiento combinado para lesiones neurológicas.
CN110392736A (zh) 2017-01-18 2019-10-29 耶达研究及发展有限公司 遗传修饰的反抑细胞及其在免疫治疗中的用途
IL250479A0 (en) 2017-02-06 2017-03-30 Sorek Rotem Genetically engineered cells expressing a disarm system that confers resistance to phages and methods for their production
IL250916A0 (en) 2017-03-02 2017-06-29 Geiger Benjamin Methods for growing t cells in culture and their use
EP3596469A1 (en) 2017-03-12 2020-01-22 Yeda Research and Development Co., Ltd. Methods of diagnosing and prognosing cancer
WO2018167780A1 (en) 2017-03-12 2018-09-20 Yeda Research And Development Co. Ltd. Methods of prognosing and treating cancer
EP3595687A1 (en) 2017-03-16 2020-01-22 Lineage Cell Therapeutics, Inc. Methods for measuring therapeutic effects of retinal disease therapies
AU2018248137B2 (en) 2017-04-05 2024-03-07 Yeda Research And Development Co. Ltd. Ex-vivo culture system and methods of using same
IL252151A0 (en) 2017-05-07 2017-07-31 Fainzilber Michael Treatment of stress disorders
CN110831966A (zh) 2017-05-10 2020-02-21 阿里埃勒科学创新有限公司 纯化抗体的方法
EP3652541B1 (en) 2017-05-21 2022-12-14 The Medical Research, Infrastructure, And Health Services Fund Of The Tel Aviv Medical Center Combination of markers for diagnosing cancer
WO2018216011A1 (en) 2017-05-23 2018-11-29 Technion Research & Development Foundation Limited Agents which inhibit gads dimerization and methods of use thereof
US20200109408A1 (en) 2017-05-31 2020-04-09 Tropic Biosciences UK Limited Methods of selecting cells comprising genome editing events
GB201708665D0 (en) 2017-05-31 2017-07-12 Tropic Biosciences Uk Ltd Compositions and methods for increasing extractability of solids from coffee beans
GB201708662D0 (en) 2017-05-31 2017-07-12 Tropic Biosciences Uk Ltd Compositions and methods for increasing shelf-life of banana
WO2018229764A1 (en) 2017-06-13 2018-12-20 Yeda Research And Development Co. Ltd. Treatment of advanced or progressive multiple sclerosis
WO2019012544A1 (en) 2017-07-13 2019-01-17 Yissum Research Development Company Of The Hebrew University Of Jerusalem Ltd. STRATEGY FOR DOUBLE PROBE DROPLETS FOR SPECIFIC DETECTION OF CIRCULATING DNA MOLECULES SPECIFIC TO A TISSUE
WO2019012543A1 (en) 2017-07-13 2019-01-17 Yissum Research Development Company Of The Hebrew University Of Jerusalem Ltd. DNA TARGETS AS SPECIFIC TISSUE METHYLATION MARKERS
US20200165671A1 (en) 2017-07-13 2020-05-28 Yissum Research Development Company Of The Hebrew University Of Jerusalem Ltd. Detecting tissue-specific dna
IL253642A0 (en) 2017-07-24 2017-09-28 Seger Rony Combined treatment for cancer
IL253984A0 (en) 2017-08-14 2017-09-28 Yeda Res & Dev Immunotherapy against aranviruses that bind through transferrin receptor 1 (tfr1
WO2019038771A1 (en) 2017-08-23 2019-02-28 Technion Research & Development Foundation Limited COMPOSITIONS AND METHODS FOR ENHANCING ALCOHOL TOLERANCE IN YEAST
KR102510576B1 (ko) 2017-09-19 2023-03-14 트로픽 바이오사이언시즈 유케이 리미티드 유전자 발현을 침묵시키기 위한 식물의 비-암호화 rna 분자의 특이성 변형
CA3082365A1 (en) 2017-11-09 2019-05-16 Pinteon Therapeutics Inc. Methods and compositions for the generation and use of humanized conformation-specific phosphorylated tau antibodies
IL255664A0 (en) 2017-11-14 2017-12-31 Shachar Idit Hematopoietic stem cells with enhanced properties
EP3716756A4 (en) 2017-11-29 2021-09-15 Weedout Ltd. COMPOSITIONS, KITS AND PROCEDURES FOR CONTROLLING WEEDS OF THE GENUS AMARANTHUS
EP3717032B1 (en) 2017-11-29 2023-02-15 Ramot at Tel-Aviv University Ltd. Methods of preventing or treating neurogenic shock
EP3716993A1 (en) 2017-12-03 2020-10-07 Yeda Research and Development Co. Ltd Treatment of an ischemic heart disease
WO2019116376A1 (en) 2017-12-17 2019-06-20 Yeda Research And Development Co. Ltd. Cop9 signalosome (csn) complex modulators and uses thereof
WO2019130313A1 (en) 2017-12-28 2019-07-04 Yissum Research Development Company Of The Hebrew University Of Jerusalem Ltd. Method to reproduce circadian rhythms on a microfluidic chip
IL257225A (en) 2018-01-29 2018-04-09 Yeda Res & Dev Treatment of sarcoma
IL257269A (en) 2018-01-31 2018-04-09 Olender Tsviya Signaling is directed to the endoplasmic reticulum
WO2019155465A1 (en) 2018-02-08 2019-08-15 Yeda Research And Development Co. Ltd. Methods of identifying and using agents for treating diseases associated with intestinal barrier dysfunction
EP3749689A1 (en) 2018-02-08 2020-12-16 Yeda Research and Development Co. Ltd Inhibitors of the 20s proteasome
US11571433B2 (en) 2018-02-23 2023-02-07 Carmel Haifa University Economic Corporation Ltd. Methods for treating memory impairment and cognitive dysfunction
WO2019167047A1 (en) 2018-02-28 2019-09-06 The Medical Research, Infrastructure and Health Services Fund of the Tel Aviv Medical Center Methods of diagnosing and treating bladder cancer
WO2019175869A1 (en) 2018-03-12 2019-09-19 Yeda Research And Development Co. Ltd Treatment of a heart disease
IL258467A (en) 2018-03-29 2018-07-04 Ilana Kolodkin Gal Methods of disrupting a biofilm and/or preventing formation of same
WO2019186569A1 (en) 2018-03-29 2019-10-03 Yeda Research And Development Co. Ltd. Use of electric field gradients to control gene expression
WO2019186558A1 (en) 2018-03-29 2019-10-03 Technion Research And Development Foundation Limited Vesicles comprising a pten inhibitor and uses of same
GB201807192D0 (en) 2018-05-01 2018-06-13 Tropic Biosciences Uk Ltd Compositions and methods for reducing caffeine content in coffee beans
IL259392A (en) 2018-05-15 2018-08-01 Yeda Res & Dev Vaccination with cancer neo-antigens
WO2019220441A1 (en) 2018-05-15 2019-11-21 Hadasit Medical Research Services And Development Ltd. Compositions and methods for treating cancer resistant to an anti-cancer agent
WO2019229745A1 (en) 2018-05-27 2019-12-05 Biolinerx Ltd. Agi-134 combined with a checkpoint inhibitor for the treatment of solid tumors
EP3802839A1 (en) 2018-06-07 2021-04-14 The State of Israel, Ministry of Agriculture & Rural Development, Agricultural Research Organization (ARO) (Volcani Center) Nucleic acid constructs and methods of using same
CA3102975A1 (en) 2018-06-07 2019-12-12 The State Of Israel, Ministry Of Agriculture & Rural Development, Agricultural Research Organization (Aro) (Volcani Center) Methods of regenerating and transforming cannabis
US11873322B2 (en) 2018-06-25 2024-01-16 Yeda Research And Development Co. Ltd. Systems and methods for increasing efficiency of genome editing
WO2020008412A1 (en) 2018-07-04 2020-01-09 Ukko Inc. Methods of de-epitoping wheat proteins and use of same for the treatment of celiac disease
EP3818529A2 (en) 2018-07-08 2021-05-12 Yeda Research and Development Co. Ltd Person-specific assessment of probiotics responsiveness
MX2021000264A (es) 2018-07-11 2021-05-12 Immunity Pharma Ltd Compuestos peptidicos y usos terapeuticos de los mismos.
CN112543773A (zh) 2018-07-11 2021-03-23 卡尔医学有限公司 SIRPα-4-1BBL变体融合蛋白及其使用方法
KR20210042430A (ko) 2018-07-31 2021-04-19 콜플랜트 리미티드 담배 형질전환 이벤트 및 이의 검출 방법 및 용도
IL261156A (en) 2018-08-14 2020-02-27 Fass Deborah Chimeric quiescin sulfhydryl oxidase (qsox1) antibodies and uses of same
WO2020039440A1 (en) 2018-08-24 2020-02-27 Yeda Research And Development Co. Ltd. Methods of modulating m2 macrophage polarization and use of same in therapy
CN113038951B (zh) 2018-09-20 2024-04-30 耶达研究及发展有限公司 治疗肌萎缩侧索硬化的方法
EP3856225A1 (en) 2018-09-25 2021-08-04 BioLineRx Ltd. Methods of selecting treatment for cxcr4-associated cancer
IL262658A (en) 2018-10-28 2020-04-30 Memorial Sloan Kettering Cancer Center Prevention of age related clonal hematopoiesis and diseases associated therewith
IL263184A (en) 2018-11-21 2020-05-31 Yarden Yosef Method of treating cancer and compositions for same
WO2020152686A1 (en) 2019-01-23 2020-07-30 Yeda Research And Development Co. Ltd. Culture media for pluripotent stem cells
IL264768A (en) 2019-02-10 2020-08-31 Sagi Irit ANTI-MATRIX METALLOPROTEINASE 7 (MMP-7) inhibitory antibody and its use
IL264854A (en) 2019-02-14 2020-08-31 Bahat Anat Spt5 inhibitors and methods of use thereof
WO2020170239A1 (en) 2019-02-21 2020-08-27 Yissum Research Development Company Of The Hebrew University Of Jerusalem Ltd. Methods of generating organoids for high throughput screening of drugs
JP2022521010A (ja) 2019-02-21 2022-04-04 イッスム・リサーチ・デベロプメント・カムパニー・オブ・ザ・ヘブリュー・ユニバシティー・オブ・エルサレム リミテッド 薬物誘導性腎毒性を減少させるための方法
MX2021010611A (es) 2019-03-05 2021-11-12 The State Of Israel Ministry Of Agriculture & Rural Development Agricultural Res Organization Aro Vo Aves con genoma editado.
GB201903521D0 (en) 2019-03-14 2019-05-01 Tropic Biosciences Uk Ltd No title
GB201903519D0 (en) 2019-03-14 2019-05-01 Tropic Biosciences Uk Ltd Introducing silencing activity to dysfunctional rna molecules and modifying their specificity against a gene of interest
GB201903520D0 (en) 2019-03-14 2019-05-01 Tropic Biosciences Uk Ltd Modifying the specificity of non-coding rna molecules for silencing genes in eukaryotic cells
IL265486A (en) 2019-03-19 2020-09-30 Yeda Res & Dev Bistable type ii opsins and uses thereof
WO2020194317A1 (en) 2019-03-28 2020-10-01 Yeda Research And Development Co. Ltd. Method of treating lipid-related disorders
WO2020202142A2 (en) 2019-03-31 2020-10-08 Yeda Research And Development Co. Ltd. Anti-viral and anti-tumoral compounds
IL266433B (en) 2019-05-02 2020-11-30 Sagi Irit Compositions comprising the propeptide of lysyl oxidase and uses thereof
US20220220559A1 (en) 2019-05-13 2022-07-14 Yeda Research And Development Co. Ltd. Cell-free mirna biomarkers for prognosis and diagnosis of neurodegenerative diseases
IL266728B (en) 2019-05-19 2020-11-30 Yeda Res & Dev Identification of recurrent mutant neopeptides
JP2022533745A (ja) 2019-05-22 2022-07-25 ハダシット メディカル リサーチ サービシーズ アンド ディベロップメント リミテッド ヒト多能性細胞を培養する方法
CA3140406A1 (en) 2019-05-28 2020-12-03 Shanghaitech University Composition and methods to treat ectodermal dysplasia 2, clouston type
WO2020250232A1 (en) 2019-06-12 2020-12-17 Hadasit Medical Research Services And Development Ltd. Methods of generating oligodendrocytes
WO2020255124A1 (en) 2019-06-16 2020-12-24 Yeda Research And Development Co. Ltd. Method for stabilizing intracellular rna
US20220251193A1 (en) 2019-06-27 2022-08-11 Ramot At Tel-Aviv University Ltd. Semaphorin 3a antibodies and uses thereof
EP3994268A1 (en) 2019-07-04 2022-05-11 Ukko Inc. De-epitoped alpha gliadin and use of same for the management of celiac disease and gluten sensitivity
CA3146248A1 (en) 2019-07-11 2021-01-14 Kahr Medical Ltd. Heterodimers and methods of use thereof
EP3999851A4 (en) 2019-07-16 2023-04-12 Ramot at Tel-Aviv University Ltd. COMPOSITIONS AND METHODS FOR TREATMENT OF TUBERCULOSIS
IL268111A (en) 2019-07-16 2021-01-31 Fainzilber Michael Methods of treating pain
WO2021019526A1 (en) 2019-07-29 2021-02-04 Yeda Research And Development Co. Ltd. Methods of treating and diagnosing lung cancer
WO2021019536A1 (en) 2019-07-30 2021-02-04 The State Of Israel, Ministry Of Agriculture & Rural Development, Agricultural Research Organization (Aro) (Volcani Center) Methods of controlling cannabinoid synthesis in plants or cells and plants and cells produced thereby
EP4014046A1 (en) 2019-08-12 2022-06-22 Yeda Research and Development Co. Ltd Dna repair blood test for predicting response of lung cancer patients to immunotherapy
WO2021033176A1 (en) 2019-08-22 2021-02-25 Ariel Scientific Innovations Ltd. Scaled-up methods for purifying antibodies
EP4021463A1 (en) 2019-08-27 2022-07-06 Yeda Research and Development Co. Ltd Treatment of bacterial vaginosis
WO2021048852A1 (en) 2019-09-11 2021-03-18 Yeda Research And Development Co. Ltd. Methods of treating breast cancer
IL269674B (en) 2019-09-25 2020-08-31 Roy Bar Ziv Assembling protein complexes on a chip
IL270306A (en) 2019-10-30 2021-05-31 Yeda Res & Dev Prevention and treatment of pre-myeloid and myeloid malignancies
US20240117386A1 (en) 2019-10-31 2024-04-11 Yeda Research And Development Co. Ltd. Engineered autotrophic bacteria for co2 conversion to organic materials
AU2020384953A1 (en) 2019-11-11 2022-06-23 Ibi-Ag Innovative Bio Insecticides Ltd. Insect control nanobodies and uses thereof
US20230022576A1 (en) 2019-11-19 2023-01-26 Protalix Ltd. Removal of constructs from transformed cells
IL270800A (en) 2019-11-20 2021-05-31 Yeda Res & Dev A method for treating Alzheimer's disease
IL271656A (en) 2019-12-22 2021-06-30 Yeda Res & Dev System and methods for identifying cells that have undergone genome editing
EP4077731A1 (en) 2019-12-22 2022-10-26 Yeda Research and Development Co. Ltd Diagnosis of frontotemporal dementia
US20230048361A1 (en) 2019-12-31 2023-02-16 Kahr Medical Ltd. Methods of culturing t cells and uses of same
IL271778A (en) 2019-12-31 2021-06-30 Ichilov Tech Ltd Methods for treating atopic dermatitis
WO2021137231A1 (en) 2019-12-31 2021-07-08 Kahr Medical Ltd. Methods of culturing t cells with a 4-1bbl fusion polypeptide and uses of same
IL272074A (en) 2020-01-15 2021-07-29 Immunity Pharma Ltd Peptide compounds and their use in methods of treating diseases
IL272194A (en) 2020-01-22 2021-07-29 Yeda Res & Dev Multi-target antibodies for use in the treatment of diseases
WO2021152584A1 (en) 2020-01-28 2021-08-05 Ariel Scientific Innovations Ltd. Methods of analyzing cell membranes
WO2021152586A1 (en) 2020-01-30 2021-08-05 Yeda Research And Development Co. Ltd. Methods of analyzing microbiome, immunoglobulin profile and physiological state
WO2021152587A1 (en) 2020-01-30 2021-08-05 Yeda Research And Development Co. Ltd. Treating acute liver disease with tlr-mik inhibitors
IL272389A (en) 2020-01-30 2021-08-31 Yeda Res & Dev Kits containing antibodies to PD-L1 and their uses in therapy
IL272390A (en) 2020-01-30 2021-08-31 Yeda Res & Dev Cancer treatment methods
CA3170374A1 (en) 2020-02-09 2021-08-12 Nlc Pharma Ltd Rapid detection test for sars-cov-2
IL272586A (en) 2020-02-10 2021-08-31 Yeda Res & Dev A method for cell cluster analysis
EP4110823A1 (en) 2020-02-26 2023-01-04 A2 Biotherapeutics, Inc. Polypeptides targeting mage-a3 peptide-mhc complexes and methods of use thereof
US20230127559A1 (en) 2020-03-16 2023-04-27 Yeda Research And Development Co. Ltd. Peptide analogs and use of same in treating diseases, disorders or conditions associated with mutant p53 protein
EP4126226A1 (en) 2020-03-29 2023-02-08 Yeda Research and Development Co. Ltd Variants of beta-glucocerebrosidase for use in treating gaucher disease
IL296698A (en) 2020-03-31 2022-11-01 Biomuse Ltd Bacteria for the prevention and treatment of smoking damage to the lungs
WO2021205444A1 (en) 2020-04-06 2021-10-14 Yeda Research And Development Co. Ltd. Methods of diagnosing cancer and predicting responsiveness to therapy
WO2021205459A1 (en) 2020-04-07 2021-10-14 Ramot At Tel-Aviv University Ltd. Cannabidiol-containing compositions and uses thereof
CA3177360A1 (en) 2020-04-13 2021-10-21 Exoprother Medical Ltd. Cell-derived vesicles comprising wild-type p53 protein for antiviral therapy
US20210322483A1 (en) 2020-04-16 2021-10-21 Ichilov Tech Ltd. Cell-derived particles presenting heterologous cd24 and use thereof in therapy
WO2021229577A1 (en) 2020-05-12 2021-11-18 Collplant Ltd. Collagen as a delivery tool for metal-based anti-viral agents
IL274811B (en) 2020-05-20 2021-05-31 Yeda Res & Dev Spatial information is developed for the analysis of individual cells
US20230293530A1 (en) 2020-06-24 2023-09-21 Yeda Research And Development Co. Ltd. Agents for sensitizing solid tumors to treatment
IL276599A (en) 2020-08-09 2022-03-01 Yeda Res & Dev T cell receptor unique to mage-a1 and its uses
IL276627A (en) 2020-08-10 2022-03-01 Yeda Res & Dev Compositions for diagnosis and treatment of coronavirus infections
WO2022038536A1 (en) 2020-08-18 2022-02-24 International Rice Research Institute Methods of increasing outcrossing rates in gramineae
IL277488A (en) 2020-09-21 2022-04-01 Yeda Res & Dev Weight regulation method
IL301503A (en) 2020-09-23 2023-05-01 Yeda res & development co ltd Methods and preparations for the treatment of the disease caused by the corona virus
IL277743A (en) 2020-10-01 2022-04-01 Yeda Res & Dev A method for diagnosing breast cancer
US20230374447A1 (en) 2020-10-05 2023-11-23 Protalix Ltd. Dicer-like knock-out plant cells
IL301970A (en) 2020-10-07 2023-06-01 Protalix Ltd A long-acting DNase
WO2022079719A1 (en) 2020-10-15 2022-04-21 Yeda Research And Development Co. Ltd. Method of treating myeloid malignancies
IL278401A (en) 2020-10-29 2022-05-01 Yeda Res & Dev Polynucleotides for editing RNA and a method for using them
KR20230107296A (ko) 2020-11-09 2023-07-14 1이 테라퓨틱스 엘티디. 촉매 서열에 기반한 박테리아 감염을 치료 또는 예방하는 방법
AU2021387979A1 (en) 2020-11-26 2023-03-30 Ukko Inc. Modified high molecular weight glutenin subunit and uses thereof
IL279559A (en) 2020-12-17 2022-07-01 Yeda Res & Dev Controlling the ubiquitination process of mlkl for disease treatment
EP4267742A2 (en) 2020-12-28 2023-11-01 1E Therapeutics, Ltd. P21 mrna targeting dnazymes
AU2021411103A1 (en) 2020-12-28 2023-07-13 1E Therapeutics, Ltd. P21 mrna target areas for silencing
EP4274886A2 (en) 2021-01-10 2023-11-15 Supermeat the Essence of Meat Ltd. Pluripotent stem cell aggregates and microtissues obtained therefrom for the cultured meat industry
US20240083843A1 (en) 2021-01-14 2024-03-14 Yeda Research And Development Co. Ltd. Methods of producing vitamin d
IL280340B (en) 2021-01-21 2022-04-01 Yeda Res & Dev Antibodies, peptides and their combinations for the treatment and prevention of corona virus infections
CA3208536A1 (en) 2021-02-18 2022-08-25 Ravid STRAUSSMAN Genetically modified bacteria for generating vaccines
WO2022175952A1 (en) 2021-02-18 2022-08-25 Yeda Research And Development Co. Ltd. Method of generating vaccines
IL281561A (en) 2021-03-16 2022-10-01 Yeda Res & Dev Methods and devices for growing mouse embryos outside the uterus
JP2024514252A (ja) 2021-03-30 2024-03-29 バイオエムエックス・リミテッド Ｐｓｅｕｄｏｍｏｎａｓバクテリオファージ及びその使用
IL282188A (en) 2021-04-08 2022-11-01 Yeda Res & Dev Combined use of ketamine and bretigabine for the treatment of psychiatric disorders
WO2022238947A1 (en) 2021-05-12 2022-11-17 Biomx Ltd. Staphylococcus bacteriophage and uses thereof
IL283563A (en) 2021-05-30 2022-12-01 Yeda Res & Dev Profiling of amps for the diagnosis, monitoring and treatment of microbiome-related diseases
CA3219233A1 (en) 2021-06-06 2022-12-15 Ravid STRAUSSMAN Combined treatment for cancer
CN117836402A (zh) 2021-06-13 2024-04-05 耶路撒冷希伯来大学伊森姆研究发展有限公司 重编程人细胞的方法
NL2028466B1 (en) 2021-06-16 2022-12-21 Sakata Holland B V Methods of sorting matthiola seeds
WO2022264076A1 (en) 2021-06-16 2022-12-22 Seedx Technologies Inc. Methods of sorting matthiola seeds
KR20240031315A (ko) 2021-07-02 2024-03-07 트로픽 바이오사이언시즈 유케이 리미티드 바나나 과실의 갈변 지연 또는 예방
WO2023003809A1 (en) 2021-07-18 2023-01-26 Gamida-Cell Ltd. Therapeutic nk cell populations
WO2023002492A1 (en) 2021-07-22 2023-01-26 Yeda Research And Development Co. Ltd. Codon optimization of nucleic acids
EP4130028A1 (en) 2021-08-03 2023-02-08 Rhazes Therapeutics Ltd Engineered tcr complex and methods of using same
WO2023012584A2 (en) 2021-08-03 2023-02-09 Genicity Limited Engineered tcr complex and methods of using same
CA3228570A1 (en) 2021-08-10 2023-02-16 Yona Geffen Engineered nk cells, methods of their production and uses thereof
GB202112866D0 (en) 2021-09-09 2021-10-27 Tropic Biosciences Uk Ltd Resistance to fusarium wilt in a banana
GB202112865D0 (en) 2021-09-09 2021-10-27 Tropic Biosciences Uk Ltd Resistance to black sigatoka disease in banana
IL286430A (en) 2021-09-14 2023-04-01 Yeda Res & Dev Multispecific antibodies for use in the treatment of diseases
WO2023105526A1 (en) 2021-12-08 2023-06-15 Yeda Research And Development Co. Ltd. Multipotent lung progenitor cells for lung regeneration
WO2023209708A1 (en) 2022-04-25 2023-11-02 Yeda Research And Development Co. Ltd. Compositions for altering cyclic adp-ribose secondary messenger signalling

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3654090A (en) * 1968-09-24 1972-04-04 Organon Method for the determination of antigens and antibodies
US3652761A (en) * 1969-09-04 1972-03-28 Corning Glass Works Immunochemical composites and antigen or antibody purification therewith

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
ACTA ENDOCRINOLOGICA=1969 *
BIOCHEM BIOPHYS ACTA=1966 *
IMMUNOCHEMISTRY=1969 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP4888387B2 (ja) * 2005-11-18 2012-02-29 三菱電機株式会社 エレベータのかご照明装置

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