ES2813407T3 - Métodos para generar células madre mesenquimales que secretan factores neurotróficos - Google Patents

Métodos para generar células madre mesenquimales que secretan factores neurotróficos Download PDF

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ES2813407T3
ES2813407T3 ES13767124T ES13767124T ES2813407T3 ES 2813407 T3 ES2813407 T3 ES 2813407T3 ES 13767124 T ES13767124 T ES 13767124T ES 13767124 T ES13767124 T ES 13767124T ES 2813407 T3 ES2813407 T3 ES 2813407T3
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growth factor
mesenchymal stem
stem cells
secrete
cells
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Yael Gothelf
Yosef Levy
Alex Burshtein
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Brainstorm Cell Therapeutics Ltd
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Abstract

Un método para generar células que secretan el factor neurotrófico derivado del cerebro (FNDC), factor neurotrófico derivado de la glía (FNDG), factor de crecimiento de hepatocitos (FCH) y factor de crecimiento endotelial vascular (VEGF), en donde dichas células no secretan factor de crecimiento nervioso (NGF) que comprende incubar una población de células madre mesenquimales (MSC) humanas, indiferenciadas, obtenidas de la médula ósea en un medio de diferenciación que comprende el factor de crecimiento básico de fibroblastos (bFGF), factor de crecimiento derivado de plaquetas (PDGF), heregulina y AMPc, en donde dicha incubación se efectúa en una sola etapa, en donde dicho medio de diferenciación carece de IBMX, generando así las células.
ES13767124T 2012-08-06 2013-08-04 Métodos para generar células madre mesenquimales que secretan factores neurotróficos Active ES2813407T3 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201261679822P 2012-08-06 2012-08-06
PCT/IL2013/050660 WO2014024183A1 (en) 2012-08-06 2013-08-04 Methods of generating mesenchymal stem cells which secrete neurotrophic factors

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ES2813407T3 true ES2813407T3 (es) 2021-03-23

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US (3) US10046010B2 (es)
EP (1) EP2880151B1 (es)
JP (2) JP6362596B2 (es)
BR (1) BR112015001435B1 (es)
CA (1) CA2877223C (es)
DK (1) DK2880151T3 (es)
ES (1) ES2813407T3 (es)
HK (1) HK1209453A1 (es)
IL (1) IL261441B (es)
WO (1) WO2014024183A1 (es)

Families Citing this family (30)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10046010B2 (en) 2012-08-06 2018-08-14 Brainstorm Cell Therapeutics Ltd. Methods of generating mesenchymal stem cells which secrete neurotrophic factors
TWI648274B (zh) 2013-02-15 2019-01-21 英商葛蘭素史克智慧財產發展有限公司 作為激酶抑制劑之雜環醯胺類 （二）
EP3105587B1 (en) 2014-02-11 2023-09-06 Brainstorm Cell Therapeutics Ltd. Method of qualifying cells
US20170247660A1 (en) * 2014-09-18 2017-08-31 National Health Research Institutes Progenitor cells, method for preparation thereof and uses thereof
CN107787363B (zh) 2015-04-24 2021-06-25 哥本哈根大学 真正的胰腺祖细胞的分离
CN105169486B (zh) * 2015-10-11 2018-04-03 温州医科大学 一种结合去细胞神经应用的神经修复材料
GB2550136A (en) * 2016-05-09 2017-11-15 Sistemic Scotland Ltd Functional prediction
EP3455367A1 (en) 2016-05-09 2019-03-20 Sistemic Scotland Ltd Functional prediction of cellular functions by means of microrna expression profiling in mesenchymal stem cells
GB2605113A (en) * 2016-05-09 2022-09-21 Sistemic Scotland Ltd Functional prediction
KR20220126789A (ko) 2016-07-18 2022-09-16 브레인스톰 셀 세라퓨틱스 리미티드 근위축성 측색 경화증 (als)의 치료 방법
IL299325A (en) * 2016-09-12 2023-02-01 Inst Nat Sante Rech Med Variants of acid alpha glucosidase and their uses
CN110402146A (zh) 2016-11-03 2019-11-01 埃克森蒂姆生物技术公司 间充质干细胞群、其产物及其用途
US20200246386A1 (en) * 2017-04-24 2020-08-06 Pluristem Ltd. Methods and compositions for treating neurological disorders
US20190054144A1 (en) * 2017-08-15 2019-02-21 Meridigen Biotech Co., Ltd. Pharmaceutical composition for treating ischemic stroke and method thereof
WO2019055729A1 (en) * 2017-09-14 2019-03-21 University Of Kansas SUBSTANCES AND METHODS FOR THE GENERATION OF THERAPEUTIC MESENCHYMAL STEM CELLS
US11471493B2 (en) * 2017-09-15 2022-10-18 Cynata Therapeutics Limited Method for treating allergic airways disease (AAD)/asthma
CA2983845C (en) 2017-10-26 2024-01-30 University Of Copenhagen Generation of glucose-responsive beta cells
WO2019198077A1 (en) 2018-04-10 2019-10-17 Brainstorm Cell Therapeutics Ltd. Cell-type specific exosomes and use thereof
US20200000886A1 (en) * 2018-06-29 2020-01-02 University Of Washington Engineering the rrm2 subunit of ribonucleotide reductase to resist degradation
CN111484978A (zh) * 2019-01-28 2020-08-04 深圳市第二人民医院 miR-140-5p过表达修饰的人脐带间充质干细胞、其治疗制剂、制备方法和应用
WO2020184729A1 (ja) 2019-03-14 2020-09-17 北海道公立大学法人札幌医科大学 筋萎縮性側索硬化症の治療用医薬組成物
KR102323056B1 (ko) * 2019-05-02 2021-11-09 에스씨엠생명과학 주식회사 hPL 함유 배지에서 배양된 중간엽 줄기세포의 배양액을 포함하는 화장료 조성물
US20220313733A1 (en) * 2019-06-11 2022-10-06 Kyoto University Method for producing renal interstitial cell
US20230133201A1 (en) * 2020-03-11 2023-05-04 Remotor Therapeutics, Inc. Methods and materials for disseminating a protein throughout the central nervous system
US20230365937A1 (en) * 2020-10-26 2023-11-16 Hadasit Medical Research Services And Development Ltd. Mesenchymal stem cells and their culture
EP4316498A1 (en) 2021-03-30 2024-02-07 Kaneka Corporation Trypsin inhibition method, and method for producing cell preparation in which same is used
CN113367123B (zh) * 2021-06-07 2022-05-13 南京三生生物技术股份有限公司 一种细胞冻存方法
CN113789333B (zh) * 2021-09-07 2023-07-14 中国人民解放军军事科学院军事医学研究院 Chi3l1在调控hUC-MSCs抑制Th17分化介导的免疫调节作用上的应用
WO2023219655A1 (en) * 2022-05-13 2023-11-16 University Of Utah Research Foundation Gja1-20k to limited cardiac arrhythmias
CN116410921B (zh) * 2023-02-09 2024-01-23 北京益华生物科技有限公司 一种人源脐带间充质干细胞诱导培养基、诱导方法及应用

Family Cites Families (38)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
NL154600B (nl) 1971-02-10 1977-09-15 Organon Nv Werkwijze voor het aantonen en bepalen van specifiek bindende eiwitten en hun corresponderende bindbare stoffen.
NL154598B (nl) 1970-11-10 1977-09-15 Organon Nv Werkwijze voor het aantonen en bepalen van laagmoleculire verbindingen en van eiwitten die deze verbindingen specifiek kunnen binden, alsmede testverpakking.
NL154599B (nl) 1970-12-28 1977-09-15 Organon Nv Werkwijze voor het aantonen en bepalen van specifiek bindende eiwitten en hun corresponderende bindbare stoffen, alsmede testverpakking.
US3901654A (en) 1971-06-21 1975-08-26 Biological Developments Receptor assays of biologically active compounds employing biologically specific receptors
US3853987A (en) 1971-09-01 1974-12-10 W Dreyer Immunological reagent and radioimmuno assay
US3867517A (en) 1971-12-21 1975-02-18 Abbott Lab Direct radioimmunoassay for antigens and their antibodies
NL171930C (nl) 1972-05-11 1983-06-01 Akzo Nv Werkwijze voor het aantonen en bepalen van haptenen, alsmede testverpakkingen.
US3850578A (en) 1973-03-12 1974-11-26 H Mcconnell Process for assaying for biologically active molecules
US3935074A (en) 1973-12-17 1976-01-27 Syva Company Antibody steric hindrance immunoassay with two antibodies
US3996345A (en) 1974-08-12 1976-12-07 Syva Company Fluorescence quenching with immunological pairs in immunoassays
US4034074A (en) 1974-09-19 1977-07-05 The Board Of Trustees Of Leland Stanford Junior University Universal reagent 2-site immunoradiometric assay using labelled anti (IgG)
US3984533A (en) 1975-11-13 1976-10-05 General Electric Company Electrophoretic method of detecting antigen-antibody reaction
US4098876A (en) 1976-10-26 1978-07-04 Corning Glass Works Reverse sandwich immunoassay
US4879219A (en) 1980-09-19 1989-11-07 General Hospital Corporation Immunoassay utilizing monoclonal high affinity IgM antibodies
US5011771A (en) 1984-04-12 1991-04-30 The General Hospital Corporation Multiepitopic immunometric assay
US4666828A (en) 1984-08-15 1987-05-19 The General Hospital Corporation Test for Huntington's disease
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4801531A (en) 1985-04-17 1989-01-31 Biotechnology Research Partners, Ltd. Apo AI/CIII genomic polymorphisms predictive of atherosclerosis
US5192553A (en) 1987-11-12 1993-03-09 Biocyte Corporation Isolation and preservation of fetal and neonatal hematopoietic stem and progenitor cells of the blood and methods of therapeutic use
US5004681B1 (en) 1987-11-12 2000-04-11 Biocyte Corp Preservation of fetal and neonatal hematopoietic stem and progenitor cells of the blood
US5272057A (en) 1988-10-14 1993-12-21 Georgetown University Method of detecting a predisposition to cancer by the use of restriction fragment length polymorphism of the gene for human poly (ADP-ribose) polymerase
US5192659A (en) 1989-08-25 1993-03-09 Genetype Ag Intron sequence analysis method for detection of adjacent and remote locus alleles as haplotypes
US5281521A (en) 1992-07-20 1994-01-25 The Trustees Of The University Of Pennsylvania Modified avidin-biotin technique
US5656498A (en) 1994-02-22 1997-08-12 Nippon Telegraph And Telephone Corporation Freeze-dried blood cells, stem cells and platelets, and manufacturing method for the same
US5955257A (en) 1997-10-21 1999-09-21 Regents Of The University Of Minnesota Infusible grade short-term cell storage medium for mononuclear cells
JP3948971B2 (ja) * 2001-08-30 2007-07-25 サンバイオ，インコーポレイティド Ｎｏｔｃｈ遺伝子の導入を用いて骨髄間質細胞を神経細胞及び骨格筋細胞に分化・誘導する方法
AU2003207064B2 (en) * 2002-02-06 2006-07-13 Sanbio, Inc. Method of differentiating/inducing bone marrow interstitial cells into nerve cells and skeleton muscle cells by transferring Notch gene
US20030215942A1 (en) 2002-02-14 2003-11-20 Stemcyte, Inc. Undesignated allogeneic stem cell bank
US20050265983A1 (en) 2002-11-17 2005-12-01 Eldad Melamed Methods, nucleic acid constructs and cells for treating neurodegenerative disorders
IL152905A0 (en) 2002-11-17 2003-06-24 Univ Ramot Dopaminergic markers induction in neuronal-like cells isolated from adult human bone marrow stromal cells: implications for novel gene therapy strategy for parkinsons disease
ES2524996T3 (es) * 2005-06-16 2014-12-16 Ramot At Tel Aviv University Ltd. Células aisladas y poblaciones que comprenden a las mismas para el tratamiento de enfermedades del SNC
WO2007066338A1 (en) 2005-12-08 2007-06-14 Ramot At Tel Aviv University Ltd. Isolated oligodendrocyte-like cells and populations comprising same for the treatment of cns diseases
EP2285951B1 (en) * 2008-05-28 2018-07-04 Ramot at Tel-Aviv University Ltd. Mesenchymal stem cells for the treatment of cns diseases
US20100184033A1 (en) 2008-07-16 2010-07-22 West Michael D Methods to accelerate the isolation of novel cell strains from pluripotent stem cells and cells obtained thereby
IL301479A (en) 2009-11-17 2023-05-01 Astellas Inst For Regenerative Medicine Methods for preparing human RPE cells and pharmaceutical preparations of human RPE cells
DE102011121982B3 (de) 2011-12-22 2013-04-18 Dietmar Hopp Stiftung GmbH Mesenchymale Stammzellen aus Fettgewebe (ADSC) zur Rekonstruktion von Haut und Weichteilgewebe
US10046010B2 (en) 2012-08-06 2018-08-14 Brainstorm Cell Therapeutics Ltd. Methods of generating mesenchymal stem cells which secrete neurotrophic factors
EP3105587B1 (en) 2014-02-11 2023-09-06 Brainstorm Cell Therapeutics Ltd. Method of qualifying cells

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