SG10201903910RA - Differentiation-induced cell population from which undifferentiated cells have been removed, use of same, and method for producing same - Google Patents

Differentiation-induced cell population from which undifferentiated cells have been removed, use of same, and method for producing same

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Publication number
SG10201903910RA
SG10201903910RA SG10201903910RA SG10201903910RA SG10201903910RA SG 10201903910R A SG10201903910R A SG 10201903910RA SG 10201903910R A SG10201903910R A SG 10201903910RA SG 10201903910R A SG10201903910R A SG 10201903910RA SG 10201903910R A SG10201903910R A SG 10201903910RA
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Singapore
Prior art keywords
same
cell population
differentiation
induced cell
undifferentiated cells
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SG10201903910RA
Inventor
Shigeo Masuda
Nagako Sougawa
Satsuki Fukushima
Shigeru Miyagawa
Yoshiki Sawa
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Univ Osaka
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Publication date
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Publication of SG10201903910RA publication Critical patent/SG10201903910RA/en

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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0603—Embryonic cells ; Embryoid bodies
    • C12N5/0606—Pluripotent embryonic cells, e.g. embryonic stem cells [ES]
    • C—CHEMISTRY; METALLURGY
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0656—Adult fibroblasts
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/33—Fibroblasts
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C—CHEMISTRY; METALLURGY
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0081—Purging biological preparations of unwanted cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0657—Cardiomyocytes; Heart cells
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0696—Artificially induced pluripotent stem cells, e.g. iPS
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/10—Cells modified by introduction of foreign genetic material
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/34—Muscles; Smooth muscle cells; Heart; Cardiac stem cells; Myoblasts; Myocytes; Cardiomyocytes
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/48—Reproductive organs
    • A61K35/54—Ovaries; Ova; Ovules; Embryos; Foetal cells; Germ cells
    • A61K35/545—Embryonic stem cells; Pluripotent stem cells; Induced pluripotent stem cells; Uncharacterised stem cells
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/2878—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against the NGF-receptor/TNF-receptor superfamily, e.g. CD27, CD30, CD40, CD95
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/20—Cytokines; Chemokines
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/50—Cell markers; Cell surface determinants
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    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/02—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from embryonic cells
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    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/45—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from artificially induced pluripotent stem cells

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
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  • Cell Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
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  • Gynecology & Obstetrics (AREA)
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  • Transplantation (AREA)
  • Molecular Biology (AREA)
  • Cardiology (AREA)
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  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

DIFFERENTIATION-INDUCED CELL POPULATION FROM WHICH UNDIFFERENTIATED CELLS HAVE BEEN REMOVED, USE OF SAME, AND METHOD FOR PRODUCING SAME 5 Provided is a cell population comprising differentiated cells obtainable by inducing differentiation of pluripotent stem cells, wherein the content ratio of undifferentiated pluripotent stem cells is 0.2% or less. 10 (No Suitable Figure)
SG10201903910RA 2014-11-07 2015-11-06 Differentiation-induced cell population from which undifferentiated cells have been removed, use of same, and method for producing same SG10201903910RA (en)

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JP2014226682 2014-11-07

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SG10201903910RA true SG10201903910RA (en) 2019-05-30

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SG11201703544QA SG11201703544QA (en) 2014-11-07 2015-11-06 Differentiation-induced cell population from which undifferentiated cells have been removed, use of same, and method for producing same

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US (2) US20170335284A1 (en)
EP (2) EP3567100A1 (en)
JP (1) JP6938154B2 (en)
KR (1) KR102639313B1 (en)
CN (1) CN107075473B (en)
AU (1) AU2015344125B2 (en)
CA (1) CA2966576C (en)
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WO (1) WO2016072519A1 (en)

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JP2018014972A (en) * 2016-07-29 2018-02-01 国立大学法人大阪大学 Method for producing a differentiation-induced cell population from which undifferentiated cells are removed
JP7123809B2 (en) * 2016-12-20 2022-08-23 住友ファーマ株式会社 Removal agent for undifferentiated iPS cells
CN106854646A (en) * 2016-12-21 2017-06-16 南开大学 A kind of mouse embryo stem cell of the low expression Lin28 of maintenance mouse embryo stem cell Naive states
CN110506109B (en) * 2017-06-05 2024-03-12 泰尔茂株式会社 Method for producing cell culture
JPWO2020067436A1 (en) * 2018-09-27 2021-08-30 国立大学法人大阪大学 Method for forming grafts of pluripotent stem cell-derived cells
JPWO2020067435A1 (en) * 2018-09-27 2021-09-09 国立大学法人大阪大学 Method for sheeting pluripotent stem cell-derived cells
WO2020067438A1 (en) 2018-09-27 2020-04-02 国立大学法人大阪大学 Sheeting method for pluripotent stem cell-derived cells
CA3119746A1 (en) * 2018-11-13 2020-05-22 Memorial Sloan-Kettering Cancer Center Stem cell-based multiplex methods and compositions
WO2020166613A1 (en) * 2019-02-13 2020-08-20 大日本住友製薬株式会社 Agent for eliminating pluripotent stem cells
KR102181316B1 (en) * 2019-05-31 2020-11-20 한국한의학연구원 Composition for inducing selective apoptosis undifferentiated human induced plurioptent stem cells comprising bee venom and the method for inducing selective apoptosis using the same
CN111979175A (en) * 2020-08-28 2020-11-24 安徽惠恩生物科技股份有限公司 Method for removing residual pluripotent stem cells in stem cell differentiation system
CN115074307B (en) * 2021-03-15 2024-01-09 广州暨南大学医药生物技术研究开发中心有限公司 Method for reprogramming fibroblast induced by small molecule compound into testis support cell and application thereof

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CA2966576A1 (en) 2016-05-12
EP3567100A1 (en) 2019-11-13
EP3216859A4 (en) 2017-09-13
CN107075473A (en) 2017-08-18
EP3216859B1 (en) 2020-03-11
JP6938154B2 (en) 2021-09-22
US20210388320A1 (en) 2021-12-16
AU2015344125B2 (en) 2022-03-24
EP3216859A1 (en) 2017-09-13
CA2966576C (en) 2021-07-20
AU2015344125A1 (en) 2017-05-25
KR20170074996A (en) 2017-06-30
JPWO2016072519A1 (en) 2017-08-17
WO2016072519A1 (en) 2016-05-12
CN107075473B (en) 2021-07-13
US20170335284A1 (en) 2017-11-23
SG11201703544QA (en) 2017-06-29
KR102639313B1 (en) 2024-02-20

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