PH12016500783A1 - Suspension and clustering of human pluripotent stem cells for differentation into pancreatic endocrine cells - Google Patents
Suspension and clustering of human pluripotent stem cells for differentation into pancreatic endocrine cellsInfo
- Publication number
- PH12016500783A1 PH12016500783A1 PH12016500783A PH12016500783A PH12016500783A1 PH 12016500783 A1 PH12016500783 A1 PH 12016500783A1 PH 12016500783 A PH12016500783 A PH 12016500783A PH 12016500783 A PH12016500783 A PH 12016500783A PH 12016500783 A1 PH12016500783 A1 PH 12016500783A1
- Authority
- PH
- Philippines
- Prior art keywords
- clustering
- suspension
- pluripotent stem
- differentation
- cells
- Prior art date
Links
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- C—CHEMISTRY; METALLURGY
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- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0676—Pancreatic cells
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
- A61P1/18—Drugs for disorders of the alimentary tract or the digestive system for pancreatic disorders, e.g. pancreatic enzymes
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P25/00—Drugs for disorders of the nervous system
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P9/00—Drugs for disorders of the cardiovascular system
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- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/0068—General culture methods using substrates
- C12N5/0075—General culture methods using substrates using microcarriers
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- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0603—Embryonic cells ; Embryoid bodies
- C12N5/0606—Pluripotent embryonic cells, e.g. embryonic stem cells [ES]
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- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
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- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
- C12N5/0657—Cardiomyocytes; Heart cells
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- C12N2500/00—Specific components of cell culture medium
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- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/10—Growth factors
- C12N2501/117—Keratinocyte growth factors (KGF-1, i.e. FGF-7; KGF-2, i.e. FGF-12)
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- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
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- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
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- C12N2501/16—Activin; Inhibin; Mullerian inhibiting substance
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- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
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- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/30—Hormones
- C12N2501/38—Hormones with nuclear receptors
- C12N2501/385—Hormones with nuclear receptors of the family of the retinoic acid recptor, e.g. RAR, RXR; Peroxisome proliferator-activated receptor [PPAR]
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- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/40—Regulators of development
- C12N2501/41—Hedgehog proteins; Cyclopamine (inhibitor)
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- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
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- C12N2501/415—Wnt; Frizzeled
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- C12N2501/42—Notch; Delta; Jagged; Serrate
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- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/70—Enzymes
- C12N2501/72—Transferases (EC 2.)
- C12N2501/727—Kinases (EC 2.7.)
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- C12N2501/999—Small molecules not provided for elsewhere
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- C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
- C12N2506/02—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from embryonic cells
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- C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
- C12N2506/45—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from artificially induced pluripotent stem cells
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- C12N2509/00—Methods for the dissociation of cells, e.g. specific use of enzymes
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- C12N2513/00—3D culture
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- C12N2531/00—Microcarriers
Abstract
The present invention provides methods of preparing aggregated pluripotent stem cell clusters for differentiation. Specifically, the invention discloses methods of differentiating pluripotent cells into beta cell, cardiac cell and neuronal cell lineages using suspension clustering. The methods involve preparing the aggregated cell clusters followed by differentiation of these clusters.
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US201361962158P | 2013-11-01 | 2013-11-01 | |
US13/998,974 US10377989B2 (en) | 2012-12-31 | 2013-12-30 | Methods for suspension cultures of human pluripotent stem cells |
PCT/US2014/042796 WO2015065537A1 (en) | 2013-11-01 | 2014-06-17 | Suspension and clustering of human pluripotent stem cells for differentiation into pancreatic endocrine cells |
Publications (1)
Publication Number | Publication Date |
---|---|
PH12016500783A1 true PH12016500783A1 (en) | 2016-06-13 |
Family
ID=53004904
Family Applications (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PH12016500783A PH12016500783A1 (en) | 2013-11-01 | 2016-04-27 | Suspension and clustering of human pluripotent stem cells for differentation into pancreatic endocrine cells |
PH12016500782A PH12016500782A1 (en) | 2013-11-01 | 2016-04-27 | Suspension and clustering of human pluripotent stem cells for differentation into pancreatic endocrine cells |
Family Applications After (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PH12016500782A PH12016500782A1 (en) | 2013-11-01 | 2016-04-27 | Suspension and clustering of human pluripotent stem cells for differentation into pancreatic endocrine cells |
Country Status (12)
Country | Link |
---|---|
EP (2) | EP3063268A4 (en) |
JP (2) | JP2016534731A (en) |
KR (4) | KR20180128529A (en) |
CN (2) | CN105793413A (en) |
AU (4) | AU2014342995C1 (en) |
BR (1) | BR112016009393A8 (en) |
CA (2) | CA2928639A1 (en) |
MX (2) | MX2016005657A (en) |
PH (2) | PH12016500783A1 (en) |
RU (2) | RU2689710C2 (en) |
SG (2) | SG11201603045VA (en) |
WO (2) | WO2015065524A2 (en) |
Families Citing this family (19)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN105793413A (en) * | 2013-11-01 | 2016-07-20 | 詹森生物科技公司 | Suspension and clustering of human pluripotent stem cells for differentiation into pancreatic endocrine cells |
CA3028422A1 (en) * | 2016-06-23 | 2017-12-28 | Tithon Biotech, Inc. | Cells expressing parathyroid hormone 1 receptor and uses thereof |
US11591571B2 (en) | 2016-11-16 | 2023-02-28 | Cynata Therapeutics Limited | Pluripotent stem cell assay |
US10391156B2 (en) | 2017-07-12 | 2019-08-27 | Viacyte, Inc. | University donor cells and related methods |
CA3070596A1 (en) * | 2017-07-21 | 2019-01-24 | Semma Therapeutics, Inc. | Re-aggregation of stem cell-derived pancreatic beta cells |
JP7000896B2 (en) * | 2017-08-23 | 2022-01-19 | 王子ホールディングス株式会社 | Cell sheet forming member, method for manufacturing cell sheet forming member, and method for manufacturing cell sheet |
EP3735457A4 (en) * | 2018-01-05 | 2021-08-25 | Platelet Biogenesis, Inc. | Compositions and methods for producing megakaryocytes |
CN112041428A (en) * | 2018-01-18 | 2020-12-04 | 新加坡科技研究局 | Method for differentiating human pluripotent stem cell lines in suspension culture |
KR102115360B1 (en) * | 2018-05-30 | 2020-05-26 | 주식회사 바이블리오테카 | Adult stem cell culture fluid and a producing method thereof |
US10724052B2 (en) | 2018-09-07 | 2020-07-28 | Crispr Therapeutics Ag | Universal donor cells |
JP2022534545A (en) | 2019-05-31 | 2022-08-01 | ダブリュ.エル.ゴア アンド アソシエイツ,インコーポレイティド | biocompatible membrane composite |
CN114206407A (en) | 2019-05-31 | 2022-03-18 | W.L.戈尔及同仁股份有限公司 | Biocompatible film composite |
AU2020282355B2 (en) | 2019-05-31 | 2023-11-02 | Viacyte, Inc. | A biocompatible membrane composite |
WO2020243668A1 (en) | 2019-05-31 | 2020-12-03 | W. L. Gore & Associates, Inc. | Cell encapsulation devices with controlled oxygen diffusion distances |
CN114375300A (en) | 2019-09-05 | 2022-04-19 | 克里斯珀医疗股份公司 | Universal donor cell |
CA3150233A1 (en) | 2019-09-05 | 2021-03-11 | Alireza Rezania | Universal donor cells |
EP4061929A1 (en) * | 2019-11-22 | 2022-09-28 | Novo Nordisk A/S | Spin-aggregated neural microspheres and the application thereof |
AU2021414617A1 (en) | 2020-12-31 | 2023-08-10 | Crispr Therapeutics Ag | Universal donor cells |
WO2022203051A1 (en) * | 2021-03-25 | 2022-09-29 | 株式会社カネカ | Method for producing pluripotent stem cell population |
Family Cites Families (20)
Publication number | Priority date | Publication date | Assignee | Title |
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KR20050044393A (en) * | 2001-11-09 | 2005-05-12 | 아르테셀 사이언스, 인크. | Endocrine pancreas differentiation of adipose tissue-derived stromal cells and uses thereof |
DK2733203T3 (en) * | 2006-08-02 | 2019-02-04 | Technion Res & Dev Foundation | PROCEDURES FOR EXPANSION OF EMBRYONAL STEM CELLS IN A SUSPENSION CULTURE |
WO2008056779A1 (en) * | 2006-11-09 | 2008-05-15 | Japan As Represented By The President Of International Medical Center Of Japan | Method for culture and passage of primate embryonic stem cell, and method for induction of differentiation of the embryonic stem cell |
WO2008148105A1 (en) * | 2007-05-25 | 2008-12-04 | Medistem Laboratories, Inc. | Endometrial stem cells and methods of making and using same |
KR20170116172A (en) * | 2007-07-01 | 2017-10-18 | 라이프스캔, 인코포레이티드 | Single pluripotent stem cell culture |
GB0800524D0 (en) * | 2008-01-14 | 2008-02-20 | Univ Brighton | Cell culture system |
WO2009116951A2 (en) * | 2008-03-17 | 2009-09-24 | Agency For Science, Technology And Research | Microcarriers for stem cell culture |
WO2010002846A1 (en) * | 2008-06-30 | 2010-01-07 | Centocor Ortho Biotech Inc. | Differentiation of pluripotent stem cells |
US8895300B2 (en) * | 2008-11-04 | 2014-11-25 | Viacyte, Inc. | Scalable primate pluripotent stem cell aggregate suspension culture and differentiation thereof |
US8008075B2 (en) * | 2008-11-04 | 2011-08-30 | Viacyte, Inc. | Stem cell aggregate suspension compositions and methods of differentiation thereof |
ES2590036T3 (en) * | 2009-08-12 | 2016-11-17 | Kyoto University | Method for inducing differentiation of pluripotent stem cells into neural precursor cells |
JP5936134B2 (en) * | 2010-06-15 | 2016-06-15 | 国立大学法人京都大学 | Selection method of human induced pluripotent stem cells |
WO2011160066A1 (en) * | 2010-06-17 | 2011-12-22 | Regents Of The University Of Minnesota | Production of insulin producing cells |
BR112013004514A2 (en) * | 2010-08-31 | 2016-06-07 | Janssen Biotech Inc | differentiation of human embryonic stem cells |
MY177150A (en) * | 2011-02-28 | 2020-09-08 | Stempeutics Res Malaysia Sdn Bhd | Isolation and expansion of adult stem cells, their therapeutic composition and uses thereof |
WO2013056072A1 (en) * | 2011-10-13 | 2013-04-18 | Wisconsin Alumni Research Foundation | Generation of cardiomyocytes from human pluripotent stem cells |
KR102203056B1 (en) * | 2011-12-22 | 2021-01-14 | 얀센 바이오테크 인코포레이티드 | Differentiation of human embryonic stem cells into single hormonal insulin positive cells |
US10519422B2 (en) * | 2012-02-29 | 2019-12-31 | Riken | Method of producing human retinal pigment epithelial cells |
EP4039798A1 (en) * | 2012-12-31 | 2022-08-10 | Janssen Biotech, Inc. | Suspension and clustering of human pluripotent cells |
CN105793413A (en) * | 2013-11-01 | 2016-07-20 | 詹森生物科技公司 | Suspension and clustering of human pluripotent stem cells for differentiation into pancreatic endocrine cells |
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2014
- 2014-05-21 CN CN201480059910.5A patent/CN105793413A/en active Pending
- 2014-05-21 EP EP14857034.4A patent/EP3063268A4/en not_active Withdrawn
- 2014-05-21 SG SG11201603045VA patent/SG11201603045VA/en unknown
- 2014-05-21 JP JP2016527338A patent/JP2016534731A/en active Pending
- 2014-05-21 CA CA2928639A patent/CA2928639A1/en not_active Abandoned
- 2014-05-21 KR KR1020187034291A patent/KR20180128529A/en active IP Right Grant
- 2014-05-21 RU RU2016121409A patent/RU2689710C2/en active
- 2014-05-21 AU AU2014342995A patent/AU2014342995C1/en not_active Ceased
- 2014-05-21 MX MX2016005657A patent/MX2016005657A/en unknown
- 2014-05-21 KR KR1020167014409A patent/KR20160079072A/en active Application Filing
- 2014-05-21 WO PCT/US2014/038993 patent/WO2015065524A2/en active Application Filing
- 2014-06-17 CA CA2928741A patent/CA2928741A1/en not_active Abandoned
- 2014-06-17 MX MX2016005658A patent/MX2016005658A/en unknown
- 2014-06-17 AU AU2014343007A patent/AU2014343007C1/en not_active Ceased
- 2014-06-17 KR KR1020167014406A patent/KR20160079071A/en active IP Right Grant
- 2014-06-17 BR BR112016009393A patent/BR112016009393A8/en not_active Application Discontinuation
- 2014-06-17 EP EP14859059.9A patent/EP3063269A4/en not_active Withdrawn
- 2014-06-17 WO PCT/US2014/042796 patent/WO2015065537A1/en active Application Filing
- 2014-06-17 KR KR1020187034483A patent/KR20180130001A/en not_active Application Discontinuation
- 2014-06-17 CN CN201480059902.0A patent/CN105683362A/en active Pending
- 2014-06-17 JP JP2016527450A patent/JP2017500013A/en active Pending
- 2014-06-17 SG SG11201603047PA patent/SG11201603047PA/en unknown
- 2014-06-17 RU RU2016121404A patent/RU2016121404A/en not_active Application Discontinuation
-
2016
- 2016-04-27 PH PH12016500783A patent/PH12016500783A1/en unknown
- 2016-04-27 PH PH12016500782A patent/PH12016500782A1/en unknown
-
2018
- 2018-07-26 AU AU2018208717A patent/AU2018208717A1/en not_active Abandoned
- 2018-07-26 AU AU2018208707A patent/AU2018208707A1/en not_active Abandoned
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