EP3063268A4 - Suspension and clustering of human pluripotent stem cells for differentiation into pancreatic endocrine cells - Google Patents
Suspension and clustering of human pluripotent stem cells for differentiation into pancreatic endocrine cells Download PDFInfo
- Publication number
- EP3063268A4 EP3063268A4 EP14857034.4A EP14857034A EP3063268A4 EP 3063268 A4 EP3063268 A4 EP 3063268A4 EP 14857034 A EP14857034 A EP 14857034A EP 3063268 A4 EP3063268 A4 EP 3063268A4
- Authority
- EP
- European Patent Office
- Prior art keywords
- clustering
- differentiation
- suspension
- pluripotent stem
- cells
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
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- C—CHEMISTRY; METALLURGY
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- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0603—Embryonic cells ; Embryoid bodies
- C12N5/0606—Pluripotent embryonic cells, e.g. embryonic stem cells [ES]
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
- A61P1/18—Drugs for disorders of the alimentary tract or the digestive system for pancreatic disorders, e.g. pancreatic enzymes
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P25/00—Drugs for disorders of the nervous system
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P9/00—Drugs for disorders of the cardiovascular system
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- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/0068—General culture methods using substrates
- C12N5/0075—General culture methods using substrates using microcarriers
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- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0618—Cells of the nervous system
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- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
- C12N5/0657—Cardiomyocytes; Heart cells
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- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0676—Pancreatic cells
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- C12N2500/00—Specific components of cell culture medium
- C12N2500/02—Atmosphere, e.g. low oxygen conditions
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- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/10—Growth factors
- C12N2501/117—Keratinocyte growth factors (KGF-1, i.e. FGF-7; KGF-2, i.e. FGF-12)
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- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
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- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
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- C12N2501/155—Bone morphogenic proteins [BMP]; Osteogenins; Osteogenic factor; Bone inducing factor
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- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
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- C12N2501/16—Activin; Inhibin; Mullerian inhibiting substance
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- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/10—Growth factors
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- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/30—Hormones
- C12N2501/38—Hormones with nuclear receptors
- C12N2501/385—Hormones with nuclear receptors of the family of the retinoic acid recptor, e.g. RAR, RXR; Peroxisome proliferator-activated receptor [PPAR]
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- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
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- C12N2501/41—Hedgehog proteins; Cyclopamine (inhibitor)
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- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
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- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/40—Regulators of development
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- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/70—Enzymes
- C12N2501/72—Transferases (EC 2.)
- C12N2501/727—Kinases (EC 2.7.)
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- C12N2501/999—Small molecules not provided for elsewhere
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- C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
- C12N2506/02—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from embryonic cells
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- C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
- C12N2506/45—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from artificially induced pluripotent stem cells
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- C12N2509/00—Methods for the dissociation of cells, e.g. specific use of enzymes
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Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US201361962158P | 2013-11-01 | 2013-11-01 | |
US13/998,974 US10377989B2 (en) | 2012-12-31 | 2013-12-30 | Methods for suspension cultures of human pluripotent stem cells |
PCT/US2014/038993 WO2015065524A2 (en) | 2013-11-01 | 2014-05-21 | Suspension and clustering of human pluripotent stem cells for differentiation into pancreatic endocrine cells |
Publications (2)
Publication Number | Publication Date |
---|---|
EP3063268A2 EP3063268A2 (en) | 2016-09-07 |
EP3063268A4 true EP3063268A4 (en) | 2017-11-29 |
Family
ID=53004904
Family Applications (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
EP14857034.4A Withdrawn EP3063268A4 (en) | 2013-11-01 | 2014-05-21 | Suspension and clustering of human pluripotent stem cells for differentiation into pancreatic endocrine cells |
EP14859059.9A Withdrawn EP3063269A4 (en) | 2013-11-01 | 2014-06-17 | Suspension and clustering of human pluripotent stem cells for differentiation into pancreatic endocrine cells |
Family Applications After (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
EP14859059.9A Withdrawn EP3063269A4 (en) | 2013-11-01 | 2014-06-17 | Suspension and clustering of human pluripotent stem cells for differentiation into pancreatic endocrine cells |
Country Status (12)
Country | Link |
---|---|
EP (2) | EP3063268A4 (en) |
JP (2) | JP2016534731A (en) |
KR (4) | KR20160079072A (en) |
CN (2) | CN105793413A (en) |
AU (4) | AU2014342995C1 (en) |
BR (1) | BR112016009393A8 (en) |
CA (2) | CA2928639A1 (en) |
MX (2) | MX2016005657A (en) |
PH (2) | PH12016500782A1 (en) |
RU (2) | RU2689710C2 (en) |
SG (2) | SG11201603045VA (en) |
WO (2) | WO2015065524A2 (en) |
Families Citing this family (19)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
KR20160079072A (en) * | 2013-11-01 | 2016-07-05 | 얀센 바이오테크 인코포레이티드 | Suspension and clustering of human pluripotent stem cells for differentiation into pancreatic endocrine cells |
US20190177687A1 (en) * | 2016-06-23 | 2019-06-13 | Tithon Biotech Inc. | Cells expressing parathyroid hormone 1 receptor and uses thereof |
CN110573607B (en) * | 2016-11-16 | 2023-10-20 | 洋蓟治疗有限公司 | Multipotent stem cell assay |
US10391156B2 (en) | 2017-07-12 | 2019-08-27 | Viacyte, Inc. | University donor cells and related methods |
EP3654996A4 (en) * | 2017-07-21 | 2021-03-24 | Semma Therapeutics, Inc. | Re-aggregation of stem cell-derived pancreatic beta cells |
JP7000896B2 (en) * | 2017-08-23 | 2022-01-19 | 王子ホールディングス株式会社 | Cell sheet forming member, method for manufacturing cell sheet forming member, and method for manufacturing cell sheet |
EP3735457A4 (en) * | 2018-01-05 | 2021-08-25 | Platelet Biogenesis, Inc. | Compositions and methods for producing megakaryocytes |
SG11202006731VA (en) * | 2018-01-18 | 2020-08-28 | Agency Science Tech & Res | Method for differentiation of human pluripotent stem cell lines in suspension culture |
KR102115360B1 (en) * | 2018-05-30 | 2020-05-26 | 주식회사 바이블리오테카 | Adult stem cell culture fluid and a producing method thereof |
US10724052B2 (en) | 2018-09-07 | 2020-07-28 | Crispr Therapeutics Ag | Universal donor cells |
EP3976237A1 (en) | 2019-05-31 | 2022-04-06 | W.L. Gore & Associates Inc. | Cell encapsulation devices with controlled oxygen diffusion distances |
US20220234006A1 (en) | 2019-05-31 | 2022-07-28 | W. L. Gore & Associates, Inc. | A biocompatible membrane composite |
WO2020243665A1 (en) | 2019-05-31 | 2020-12-03 | W. L. Gore & Associates, Inc. | A biocompatible membrane composite |
AU2020284245B2 (en) | 2019-05-31 | 2023-10-05 | Viacyte, Inc. | A biocompatible membrane composite |
EP4025224A1 (en) | 2019-09-05 | 2022-07-13 | CRISPR Therapeutics AG | Universal donor cells |
CA3150235A1 (en) | 2019-09-05 | 2021-03-11 | Alireza Rezania | Universal donor cells |
EP4061929A1 (en) * | 2019-11-22 | 2022-09-28 | Novo Nordisk A/S | Spin-aggregated neural microspheres and the application thereof |
JP2024503290A (en) | 2020-12-31 | 2024-01-25 | クリスパー セラピューティクス アクチェンゲゼルシャフト | universal donor cells |
WO2022203051A1 (en) * | 2021-03-25 | 2022-09-29 | 株式会社カネカ | Method for producing pluripotent stem cell population |
Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2011019092A1 (en) * | 2009-08-12 | 2011-02-17 | Kyoto University | Method for inducing differentiation of pluripotent stem cells into neural precursor cells |
WO2013056072A1 (en) * | 2011-10-13 | 2013-04-18 | Wisconsin Alumni Research Foundation | Generation of cardiomyocytes from human pluripotent stem cells |
WO2014106141A1 (en) * | 2012-12-31 | 2014-07-03 | Janssen Biotech, Inc. | Suspension and clustering of human pluripotent cells for differentiation into pancreatic endocrine cells |
Family Cites Families (17)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
MXPA04004311A (en) * | 2001-11-09 | 2005-03-31 | Artecel Sciences Inc | Endocrine pancreas differentiation of adipose tissue-derived stromal cells and uses thereof. |
ES2874223T3 (en) * | 2006-08-02 | 2021-11-04 | Technion Res & Dev Foundation | Embryonic stem cell expansion methods in suspension culture |
WO2008056779A1 (en) * | 2006-11-09 | 2008-05-15 | Japan As Represented By The President Of International Medical Center Of Japan | Method for culture and passage of primate embryonic stem cell, and method for induction of differentiation of the embryonic stem cell |
WO2008148105A1 (en) * | 2007-05-25 | 2008-12-04 | Medistem Laboratories, Inc. | Endometrial stem cells and methods of making and using same |
WO2009006399A1 (en) * | 2007-07-01 | 2009-01-08 | Lifescan | Single pluripotent stem cell culture |
GB0800524D0 (en) * | 2008-01-14 | 2008-02-20 | Univ Brighton | Cell culture system |
WO2009116951A2 (en) * | 2008-03-17 | 2009-09-24 | Agency For Science, Technology And Research | Microcarriers for stem cell culture |
EP2942392B1 (en) * | 2008-06-30 | 2018-10-03 | Janssen Biotech, Inc. | Differentiation of pluripotent stem cells |
US8008075B2 (en) * | 2008-11-04 | 2011-08-30 | Viacyte, Inc. | Stem cell aggregate suspension compositions and methods of differentiation thereof |
US8895300B2 (en) * | 2008-11-04 | 2014-11-25 | Viacyte, Inc. | Scalable primate pluripotent stem cell aggregate suspension culture and differentiation thereof |
US8932857B2 (en) * | 2010-06-15 | 2015-01-13 | Kyoto University | Method for selecting reduced differentiation resistance human induced pluripotent stem cells |
WO2011160066A1 (en) * | 2010-06-17 | 2011-12-22 | Regents Of The University Of Minnesota | Production of insulin producing cells |
PL2853589T3 (en) * | 2010-08-31 | 2018-05-30 | Janssen Biotech, Inc | Differentiation of human embryonic stem cells |
MY177150A (en) * | 2011-02-28 | 2020-09-08 | Stempeutics Res Malaysia Sdn Bhd | Isolation and expansion of adult stem cells, their therapeutic composition and uses thereof |
RU2668798C2 (en) * | 2011-12-22 | 2018-10-02 | Янссен Байотек, Инк. | Methods for in vitro of step-by-step differentiation of pluripotent cells |
US10519422B2 (en) * | 2012-02-29 | 2019-12-31 | Riken | Method of producing human retinal pigment epithelial cells |
KR20160079072A (en) * | 2013-11-01 | 2016-07-05 | 얀센 바이오테크 인코포레이티드 | Suspension and clustering of human pluripotent stem cells for differentiation into pancreatic endocrine cells |
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2014
- 2014-05-21 KR KR1020167014409A patent/KR20160079072A/en active Application Filing
- 2014-05-21 WO PCT/US2014/038993 patent/WO2015065524A2/en active Application Filing
- 2014-05-21 CN CN201480059910.5A patent/CN105793413A/en active Pending
- 2014-05-21 KR KR1020187034291A patent/KR20180128529A/en active IP Right Grant
- 2014-05-21 SG SG11201603045VA patent/SG11201603045VA/en unknown
- 2014-05-21 CA CA2928639A patent/CA2928639A1/en not_active Abandoned
- 2014-05-21 EP EP14857034.4A patent/EP3063268A4/en not_active Withdrawn
- 2014-05-21 JP JP2016527338A patent/JP2016534731A/en active Pending
- 2014-05-21 MX MX2016005657A patent/MX2016005657A/en unknown
- 2014-05-21 AU AU2014342995A patent/AU2014342995C1/en not_active Ceased
- 2014-05-21 RU RU2016121409A patent/RU2689710C2/en active
- 2014-06-17 AU AU2014343007A patent/AU2014343007C1/en not_active Ceased
- 2014-06-17 BR BR112016009393A patent/BR112016009393A8/en not_active Application Discontinuation
- 2014-06-17 KR KR1020187034483A patent/KR20180130001A/en not_active Application Discontinuation
- 2014-06-17 CN CN201480059902.0A patent/CN105683362A/en active Pending
- 2014-06-17 CA CA2928741A patent/CA2928741A1/en not_active Abandoned
- 2014-06-17 SG SG11201603047PA patent/SG11201603047PA/en unknown
- 2014-06-17 KR KR1020167014406A patent/KR20160079071A/en active IP Right Grant
- 2014-06-17 RU RU2016121404A patent/RU2016121404A/en not_active Application Discontinuation
- 2014-06-17 WO PCT/US2014/042796 patent/WO2015065537A1/en active Application Filing
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