GB1549069A - Enzyme linked immunoassay - Google Patents
Enzyme linked immunoassayInfo
- Publication number
- GB1549069A GB1549069A GB51709/76A GB5170976A GB1549069A GB 1549069 A GB1549069 A GB 1549069A GB 51709/76 A GB51709/76 A GB 51709/76A GB 5170976 A GB5170976 A GB 5170976A GB 1549069 A GB1549069 A GB 1549069A
- Authority
- GB
- United Kingdom
- Prior art keywords
- antigen
- enzyme linked
- linked immunoassay
- insoluble
- sample
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54306—Solid-phase reaction mechanisms
Abstract
In order to determine the presence or the quantity of an antigen, an antigen (1) in insoluble form and two antibodies (2) and (3), of which the second is labelled with an enzyme, are brought into contact in the sample to be analysed. The second antibody (3) binds to the insoluble antigen (1) in a proportion which is the inverse of the quantity of antigen present in the sample. The insoluble fraction and the soluble fraction are separated and the enzymatic activity of one or the other is measured.
Priority Applications (27)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
GB51709/76A GB1549069A (en) | 1976-12-10 | 1976-12-10 | Enzyme linked immunoassay |
JP14733977A JPS53101522A (en) | 1976-12-10 | 1977-12-09 | Improved enzyme binding immune analysing method |
CH1516677A CH628739A5 (en) | 1976-12-10 | 1977-12-09 | Process of immunological assay using a bound enzyme, and means for implementing the process |
IL53576A IL53576A (en) | 1976-12-10 | 1977-12-09 | Enzyme-linked immunoassay for detection of assay of antiges |
AR270301A AR226805A1 (en) | 1976-12-10 | 1977-12-09 | REAGENT TO DETERMINE THE PRESENCE OR AMOUNTS OF AN ANTIGEN T3 OR HCG IN A LIQUID SAMPLE FOR IMMUNOANALYSIS WITH LINKED ENZYMES |
ES464929A ES464929A1 (en) | 1976-12-10 | 1977-12-09 | Enzyme linked immunoassay |
BR7708181A BR7708181A (en) | 1976-12-10 | 1977-12-09 | ENZYME-IMMUNE-ASSESSMENT PROCESS AND SET OF MATERIALS FOR USE IN PROCESSING A PROCESS |
NO774240A NO774240L (en) | 1976-12-10 | 1977-12-09 | PROCEDURE FOR DETECTION AND DETERMINATION OF ANTIGENES |
FR7737194A FR2373795A1 (en) | 1976-12-10 | 1977-12-09 | IMMUNOLOGICAL DOSAGE BY FIXED ENZYME |
NL7713692A NL7713692A (en) | 1976-12-10 | 1977-12-09 | IMPROVEMENTS IN AND RELATING TO ENZYME-LINKED IMMUNO ANALYZES. |
GR54943A GR63580B (en) | 1976-12-10 | 1977-12-09 | Improvements in and relating to enzyme-linked immunoassay |
IT30554/77A IT1109485B (en) | 1976-12-10 | 1977-12-09 | IMPROVEMENTS RELATING TO THE IMMUNOLOGICAL TEST WITH ENZYME |
HU77EA179A HU179956B (en) | 1976-12-10 | 1977-12-09 | Process for the immunological determination of antigens by means of enzyme marking |
NZ185927A NZ185927A (en) | 1976-12-10 | 1977-12-09 | Detection or assay of antigen using two antibodies one being enzyme-labelled |
FI773718A FI773718A (en) | 1976-12-10 | 1977-12-09 | ENVIRONMENTAL IMMUNITY TEST FOR MATERIALS FOR OIL FUEL |
CS778265A CS208739B2 (en) | 1976-12-10 | 1977-12-09 | Method of immunological determination of the antidotes by means of the bonded enzyme |
TR20650A TR20650A (en) | 1976-12-10 | 1977-12-09 | ENZYME-MOUNTED IMMUNO DETERMINATION OR RELATED REPORT |
CA292,743A CA1110165A (en) | 1976-12-10 | 1977-12-09 | Double antibody immunoassay using enzyme-labelled antibody and solid phase antigen |
DK550077A DK550077A (en) | 1976-12-10 | 1977-12-09 | PROCEDURE FOR ENZYMO-COUPLED IMMUNALYSIS AND THE REAGENT KIT FOR USE IN ITS EXERCISE |
SE7714034A SE7714034L (en) | 1976-12-10 | 1977-12-09 | PROCEDURE FOR ENZYME COUPLE IMMUNO ANALYSIS |
BE183341A BE861711A (en) | 1976-12-10 | 1977-12-09 | IMMUNOLOGICAL DOSAGE BY FIXED ENZYME |
PT67384A PT67384B (en) | 1976-12-10 | 1977-12-09 | Improvements in and relating to enzyme-linked immunoassay |
DE19772755008 DE2755008A1 (en) | 1976-12-10 | 1977-12-09 | PROCEDURE FOR IMMUNOLOGICAL DETERMINATION USING ENZYME MARKERS |
AU31395/77A AU517266B2 (en) | 1976-12-10 | 1977-12-09 | Enzyme-linked immunoassay |
ES468934A ES468934A1 (en) | 1976-12-10 | 1978-04-19 | Enzyme linked immunoassay |
HK138/81A HK13881A (en) | 1976-12-10 | 1981-04-09 | Improvements in and relating to enzyme-linked immunoassay |
MY367/81A MY8100367A (en) | 1976-12-10 | 1981-12-30 | Improvements in and relating to enzyme-linked immunoassay |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
GB51709/76A GB1549069A (en) | 1976-12-10 | 1976-12-10 | Enzyme linked immunoassay |
Publications (1)
Publication Number | Publication Date |
---|---|
GB1549069A true GB1549069A (en) | 1979-08-01 |
Family
ID=10461077
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
GB51709/76A Expired GB1549069A (en) | 1976-12-10 | 1976-12-10 | Enzyme linked immunoassay |
Country Status (26)
Country | Link |
---|---|
JP (1) | JPS53101522A (en) |
AR (1) | AR226805A1 (en) |
AU (1) | AU517266B2 (en) |
BE (1) | BE861711A (en) |
BR (1) | BR7708181A (en) |
CA (1) | CA1110165A (en) |
CH (1) | CH628739A5 (en) |
CS (1) | CS208739B2 (en) |
DE (1) | DE2755008A1 (en) |
DK (1) | DK550077A (en) |
ES (2) | ES464929A1 (en) |
FI (1) | FI773718A (en) |
FR (1) | FR2373795A1 (en) |
GB (1) | GB1549069A (en) |
GR (1) | GR63580B (en) |
HK (1) | HK13881A (en) |
HU (1) | HU179956B (en) |
IL (1) | IL53576A (en) |
IT (1) | IT1109485B (en) |
MY (1) | MY8100367A (en) |
NL (1) | NL7713692A (en) |
NO (1) | NO774240L (en) |
NZ (1) | NZ185927A (en) |
PT (1) | PT67384B (en) |
SE (1) | SE7714034L (en) |
TR (1) | TR20650A (en) |
Cited By (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0062892A1 (en) * | 1981-04-13 | 1982-10-20 | American Hoechst Corporation | Single incubation immunochemical assay for creatin phosphokinase MB |
EP0084531A1 (en) * | 1981-07-31 | 1983-08-03 | Sinai School Medicine | Assay for viruses. |
US4467031A (en) * | 1981-08-21 | 1984-08-21 | Hoffmann-La Roche Inc. | Enzyme-immunoassay for carcinoembryonic antigen |
US4808522A (en) * | 1986-04-24 | 1989-02-28 | Moskovsky Gosudarstvennx Universitet Imeni M. V. Lomonoso VA | Enzyme immunoassays using inorganic pyrophosphatase |
AT388618B (en) * | 1987-05-05 | 1989-08-10 | Binder Bernd Dr | METHOD FOR QUANTITATIVE DETERMINATION OF FUNCTION AND ANTIGENT CONCENTRATION OF A SUBSTANCE CONTAINED IN A BIOLOGICAL LIQUID |
EP1992636A3 (en) * | 1999-11-12 | 2009-04-08 | Amgen Inc. | Process for correction of a disulfide misfold in Fc molecules |
Families Citing this family (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS576363A (en) * | 1980-06-13 | 1982-01-13 | Takeda Chem Ind Ltd | Production of specific antibody |
CA1160566A (en) * | 1980-04-25 | 1984-01-17 | Harald Gallati | Immunological determination method |
JPS58151559A (en) * | 1982-03-05 | 1983-09-08 | Takeda Chem Ind Ltd | Immunochemical measuring method of human villous gonadotropin and reagent therefor |
US4704356A (en) * | 1984-03-27 | 1987-11-03 | Rush-Presbyterian-St. Luke's Medical Center | Method of diagnosing cartilage tissue abnormalities |
US4734362A (en) * | 1986-02-03 | 1988-03-29 | Cambridge Bioscience Corporation | Process for purifying recombinant proteins, and products thereof |
DE3807440A1 (en) * | 1988-03-07 | 1989-09-21 | Progen Biotechnik Gmbh | Method for the immunological detection of substances, and a composition and a test kit |
DE19508264C1 (en) * | 1995-03-08 | 1996-02-01 | Klose Werner Dipl Ing Fh | Measuring contours, esp. in road surface |
Family Cites Families (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
NL154600B (en) * | 1971-02-10 | 1977-09-15 | Organon Nv | METHOD FOR THE DETERMINATION AND DETERMINATION OF SPECIFIC BINDING PROTEINS AND THEIR CORRESPONDING BINDABLE SUBSTANCES. |
NL154599B (en) * | 1970-12-28 | 1977-09-15 | Organon Nv | PROCEDURE FOR DETERMINING AND DETERMINING SPECIFIC BINDING PROTEINS AND THEIR CORRESPONDING BINDABLE SUBSTANCES, AND TEST PACKAGING. |
US4002532A (en) * | 1974-10-21 | 1977-01-11 | Weltman Joel K | Enzyme conjugates |
US3951748A (en) * | 1974-11-11 | 1976-04-20 | Medical Products, Inc. | Sensitized matrix for detection of disease |
DK140815B (en) * | 1975-06-10 | 1979-11-19 | Weeke Bengt | A method for detecting or determining antibodies in body fluids by known antigens or for determining antigens by known antibodies from body fluids and a means for use in practicing the method. |
-
1976
- 1976-12-10 GB GB51709/76A patent/GB1549069A/en not_active Expired
-
1977
- 1977-12-09 GR GR54943A patent/GR63580B/en unknown
- 1977-12-09 SE SE7714034A patent/SE7714034L/en not_active Application Discontinuation
- 1977-12-09 CA CA292,743A patent/CA1110165A/en not_active Expired
- 1977-12-09 AU AU31395/77A patent/AU517266B2/en not_active Expired
- 1977-12-09 TR TR20650A patent/TR20650A/en unknown
- 1977-12-09 AR AR270301A patent/AR226805A1/en active
- 1977-12-09 CS CS778265A patent/CS208739B2/en unknown
- 1977-12-09 NL NL7713692A patent/NL7713692A/en not_active Application Discontinuation
- 1977-12-09 IT IT30554/77A patent/IT1109485B/en active
- 1977-12-09 CH CH1516677A patent/CH628739A5/en not_active IP Right Cessation
- 1977-12-09 ES ES464929A patent/ES464929A1/en not_active Expired
- 1977-12-09 JP JP14733977A patent/JPS53101522A/en active Pending
- 1977-12-09 BE BE183341A patent/BE861711A/en not_active IP Right Cessation
- 1977-12-09 IL IL53576A patent/IL53576A/en unknown
- 1977-12-09 BR BR7708181A patent/BR7708181A/en unknown
- 1977-12-09 FR FR7737194A patent/FR2373795A1/en active Granted
- 1977-12-09 HU HU77EA179A patent/HU179956B/en unknown
- 1977-12-09 DE DE19772755008 patent/DE2755008A1/en not_active Ceased
- 1977-12-09 DK DK550077A patent/DK550077A/en not_active Application Discontinuation
- 1977-12-09 NO NO774240A patent/NO774240L/en unknown
- 1977-12-09 NZ NZ185927A patent/NZ185927A/en unknown
- 1977-12-09 FI FI773718A patent/FI773718A/en not_active Application Discontinuation
- 1977-12-09 PT PT67384A patent/PT67384B/en unknown
-
1978
- 1978-04-19 ES ES468934A patent/ES468934A1/en not_active Expired
-
1981
- 1981-04-09 HK HK138/81A patent/HK13881A/en unknown
- 1981-12-30 MY MY367/81A patent/MY8100367A/en unknown
Cited By (10)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0062892A1 (en) * | 1981-04-13 | 1982-10-20 | American Hoechst Corporation | Single incubation immunochemical assay for creatin phosphokinase MB |
EP0084531A1 (en) * | 1981-07-31 | 1983-08-03 | Sinai School Medicine | Assay for viruses. |
EP0084531A4 (en) * | 1981-07-31 | 1983-12-19 | Sinai School Medicine | Assay for viruses. |
US4588680A (en) * | 1981-07-31 | 1986-05-13 | Mount Sinai School Of Medicine Of The City University Of New York | Assay for viruses |
US4467031A (en) * | 1981-08-21 | 1984-08-21 | Hoffmann-La Roche Inc. | Enzyme-immunoassay for carcinoembryonic antigen |
US4808522A (en) * | 1986-04-24 | 1989-02-28 | Moskovsky Gosudarstvennx Universitet Imeni M. V. Lomonoso VA | Enzyme immunoassays using inorganic pyrophosphatase |
AT388618B (en) * | 1987-05-05 | 1989-08-10 | Binder Bernd Dr | METHOD FOR QUANTITATIVE DETERMINATION OF FUNCTION AND ANTIGENT CONCENTRATION OF A SUBSTANCE CONTAINED IN A BIOLOGICAL LIQUID |
US5298401A (en) * | 1987-05-05 | 1994-03-29 | Bernd Binder | Process for the quantitative determination of the function and antigenic concentration of a substance contained in a biological liquid |
EP1992636A3 (en) * | 1999-11-12 | 2009-04-08 | Amgen Inc. | Process for correction of a disulfide misfold in Fc molecules |
US7723490B2 (en) | 1999-11-12 | 2010-05-25 | Amgen Inc. | Process for correction of a disulfide misfold in Fc molecules |
Also Published As
Publication number | Publication date |
---|---|
FR2373795B1 (en) | 1982-09-10 |
ES464929A1 (en) | 1979-01-01 |
ES468934A1 (en) | 1978-12-16 |
BE861711A (en) | 1978-03-31 |
AU517266B2 (en) | 1981-07-16 |
PT67384B (en) | 1979-05-18 |
FI773718A (en) | 1978-06-11 |
HK13881A (en) | 1981-04-16 |
PT67384A (en) | 1978-01-01 |
BR7708181A (en) | 1978-07-11 |
SE7714034L (en) | 1978-06-11 |
AU3139577A (en) | 1979-06-14 |
IT1109485B (en) | 1985-12-16 |
NZ185927A (en) | 1980-02-21 |
JPS53101522A (en) | 1978-09-05 |
DE2755008A1 (en) | 1978-06-15 |
HU179956B (en) | 1983-01-28 |
NL7713692A (en) | 1978-06-13 |
IL53576A0 (en) | 1978-03-10 |
DK550077A (en) | 1978-06-11 |
CS208739B2 (en) | 1981-09-15 |
IL53576A (en) | 1981-07-31 |
AR226805A1 (en) | 1982-08-31 |
CH628739A5 (en) | 1982-03-15 |
NO774240L (en) | 1978-06-13 |
CA1110165A (en) | 1981-10-06 |
GR63580B (en) | 1979-11-22 |
FR2373795A1 (en) | 1978-07-07 |
MY8100367A (en) | 1981-12-31 |
TR20650A (en) | 1982-03-24 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
PS | Patent sealed [section 19, patents act 1949] | ||
PCNP | Patent ceased through non-payment of renewal fee |