FR2373795A1 - IMMUNOLOGICAL DOSAGE BY FIXED ENZYME - Google Patents

IMMUNOLOGICAL DOSAGE BY FIXED ENZYME

Info

Publication number
FR2373795A1
FR2373795A1 FR7737194A FR7737194A FR2373795A1 FR 2373795 A1 FR2373795 A1 FR 2373795A1 FR 7737194 A FR7737194 A FR 7737194A FR 7737194 A FR7737194 A FR 7737194A FR 2373795 A1 FR2373795 A1 FR 2373795A1
Authority
FR
France
Prior art keywords
antigen
antibody
fixed enzyme
phase
relates
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
FR7737194A
Other languages
French (fr)
Other versions
FR2373795B1 (en
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Pfizer Italia SRL
Original Assignee
Carlo Erba SpA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Carlo Erba SpA filed Critical Carlo Erba SpA
Publication of FR2373795A1 publication Critical patent/FR2373795A1/en
Application granted granted Critical
Publication of FR2373795B1 publication Critical patent/FR2373795B1/fr
Granted legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54306—Solid-phase reaction mechanisms

Abstract

L'invention concerne la biochimie. Elle a pour objet un procédé de mise en évidence ou de dosage d'un antigène par contact dans un milieu liquide simultanément ou par stades, 1 d'un antigène porté par un support insoluble, 2 d'un échantillon contenant l'antigène à rechercher 3 d'un premier anticorps, et 4 d'un second anticorps marqué au moyen d'un enzyme, le second anticorps se fixant ainsi sur la phase insoluble en raison inverse de la quantité d'antigène à rechercher, l'activité enzymatique pouvant être ensuite determinée sur la phase solide ou la phase liquide. Le procédé est préféré pour les analyses de dépistage.The invention relates to biochemistry. It relates to a method for demonstrating or assaying an antigen by contact in a liquid medium simultaneously or in stages, 1 of an antigen carried by an insoluble support, 2 of a sample containing the antigen to be sought 3 of a first antibody, and 4 of a second antibody labeled with an enzyme, the second antibody thus binding to the insoluble phase in inverse proportion to the amount of antigen to be tested, the enzymatic activity being able to be then determined on the solid phase or the liquid phase. The method is preferred for screening assays.

FR7737194A 1976-12-10 1977-12-09 IMMUNOLOGICAL DOSAGE BY FIXED ENZYME Granted FR2373795A1 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
GB51709/76A GB1549069A (en) 1976-12-10 1976-12-10 Enzyme linked immunoassay

Publications (2)

Publication Number Publication Date
FR2373795A1 true FR2373795A1 (en) 1978-07-07
FR2373795B1 FR2373795B1 (en) 1982-09-10

Family

ID=10461077

Family Applications (1)

Application Number Title Priority Date Filing Date
FR7737194A Granted FR2373795A1 (en) 1976-12-10 1977-12-09 IMMUNOLOGICAL DOSAGE BY FIXED ENZYME

Country Status (26)

Country Link
JP (1) JPS53101522A (en)
AR (1) AR226805A1 (en)
AU (1) AU517266B2 (en)
BE (1) BE861711A (en)
BR (1) BR7708181A (en)
CA (1) CA1110165A (en)
CH (1) CH628739A5 (en)
CS (1) CS208739B2 (en)
DE (1) DE2755008A1 (en)
DK (1) DK550077A (en)
ES (2) ES464929A1 (en)
FI (1) FI773718A (en)
FR (1) FR2373795A1 (en)
GB (1) GB1549069A (en)
GR (1) GR63580B (en)
HK (1) HK13881A (en)
HU (1) HU179956B (en)
IL (1) IL53576A (en)
IT (1) IT1109485B (en)
MY (1) MY8100367A (en)
NL (1) NL7713692A (en)
NO (1) NO774240L (en)
NZ (1) NZ185927A (en)
PT (1) PT67384B (en)
SE (1) SE7714034L (en)
TR (1) TR20650A (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0233044A2 (en) * 1986-02-03 1987-08-19 Cambridge Biotech Corporation Process for purifying recombinant proteins, and use of products thereof

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS576363A (en) * 1980-06-13 1982-01-13 Takeda Chem Ind Ltd Production of specific antibody
CA1160566A (en) * 1980-04-25 1984-01-17 Harald Gallati Immunological determination method
ES511156A0 (en) * 1981-04-13 1983-08-01 Hoechst Co American A METHOD OF DETERMINING THE PRESENCE OF AN ANTIGEN IN A LIQUID MEDIUM SUSPECTED TO CONTAIN IT.
EP0084531B1 (en) * 1981-07-31 1987-05-20 Mount Sinai School Of Medicine Of The City University Of New York Assay for viruses
DE3263249D1 (en) * 1981-08-21 1985-05-30 Hoffmann La Roche Method for the determination of carcinoembryonic antigen (cea) and suitable antibody solution for the determination
JPS58151559A (en) * 1982-03-05 1983-09-08 Takeda Chem Ind Ltd Immunochemical measuring method of human villous gonadotropin and reagent therefor
US4704356A (en) * 1984-03-27 1987-11-03 Rush-Presbyterian-St. Luke's Medical Center Method of diagnosing cartilage tissue abnormalities
CH670709A5 (en) * 1986-04-24 1989-06-30 Univ Moskovsk
AT388618B (en) * 1987-05-05 1989-08-10 Binder Bernd Dr METHOD FOR QUANTITATIVE DETERMINATION OF FUNCTION AND ANTIGENT CONCENTRATION OF A SUBSTANCE CONTAINED IN A BIOLOGICAL LIQUID
DE3807440A1 (en) * 1988-03-07 1989-09-21 Progen Biotechnik Gmbh Method for the immunological detection of substances, and a composition and a test kit
DE19508264C1 (en) * 1995-03-08 1996-02-01 Klose Werner Dipl Ing Fh Measuring contours, esp. in road surface
US6808902B1 (en) 1999-11-12 2004-10-26 Amgen Inc. Process for correction of a disulfide misfold in IL-1Ra Fc fusion molecules

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2126760A5 (en) * 1971-02-10 1972-10-06 Organon Nv
US3839153A (en) * 1970-12-28 1974-10-01 Akzona Inc Process for the detection and determination of specific binding proteins and their corresponding bindable substances
US3951748A (en) * 1974-11-11 1976-04-20 Medical Products, Inc. Sensitized matrix for detection of disease
FR2314499A1 (en) * 1975-06-10 1977-01-07 Weeke Bengt METHOD FOR DETERMINING ANTIBODIES IN BODY FLUIDS USING KNOWN ANTIGENS OR FOR DETERMINING ANTIGENS USING KNOWN ANTIBODIES FROM BODY FLUIDS
US4002532A (en) * 1974-10-21 1977-01-11 Weltman Joel K Enzyme conjugates

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3839153A (en) * 1970-12-28 1974-10-01 Akzona Inc Process for the detection and determination of specific binding proteins and their corresponding bindable substances
FR2126760A5 (en) * 1971-02-10 1972-10-06 Organon Nv
US4002532A (en) * 1974-10-21 1977-01-11 Weltman Joel K Enzyme conjugates
US3951748A (en) * 1974-11-11 1976-04-20 Medical Products, Inc. Sensitized matrix for detection of disease
FR2314499A1 (en) * 1975-06-10 1977-01-07 Weeke Bengt METHOD FOR DETERMINING ANTIBODIES IN BODY FLUIDS USING KNOWN ANTIGENS OR FOR DETERMINING ANTIGENS USING KNOWN ANTIBODIES FROM BODY FLUIDS

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0233044A2 (en) * 1986-02-03 1987-08-19 Cambridge Biotech Corporation Process for purifying recombinant proteins, and use of products thereof
EP0233044A3 (en) * 1986-02-03 1990-07-25 Cambridge Bioscience Corporation Process for purifying recombinant proteins, and use of products thereof
EP0596459A2 (en) * 1986-02-03 1994-05-11 Cambridge Biotech Corporation Process for purifying recombinant proteins, and use of products thereof
EP0596459A3 (en) * 1986-02-03 1994-06-01 Cambridge Biotech Corporation Process for purifying recombinant proteins, and use of products thereof

Also Published As

Publication number Publication date
CS208739B2 (en) 1981-09-15
NZ185927A (en) 1980-02-21
BR7708181A (en) 1978-07-11
IL53576A (en) 1981-07-31
MY8100367A (en) 1981-12-31
PT67384B (en) 1979-05-18
DE2755008A1 (en) 1978-06-15
GB1549069A (en) 1979-08-01
AR226805A1 (en) 1982-08-31
FI773718A (en) 1978-06-11
PT67384A (en) 1978-01-01
FR2373795B1 (en) 1982-09-10
IT1109485B (en) 1985-12-16
AU3139577A (en) 1979-06-14
AU517266B2 (en) 1981-07-16
GR63580B (en) 1979-11-22
HK13881A (en) 1981-04-16
SE7714034L (en) 1978-06-11
JPS53101522A (en) 1978-09-05
NL7713692A (en) 1978-06-13
BE861711A (en) 1978-03-31
HU179956B (en) 1983-01-28
CA1110165A (en) 1981-10-06
CH628739A5 (en) 1982-03-15
ES468934A1 (en) 1978-12-16
DK550077A (en) 1978-06-11
NO774240L (en) 1978-06-13
ES464929A1 (en) 1979-01-01
IL53576A0 (en) 1978-03-10
TR20650A (en) 1982-03-24

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Legal Events

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CD Change of name or company name
ST Notification of lapse