GB2001172A - Competitive protein binding assay - Google Patents

Competitive protein binding assay

Info

Publication number
GB2001172A
GB2001172A GB7829892A GB7829892A GB2001172A GB 2001172 A GB2001172 A GB 2001172A GB 7829892 A GB7829892 A GB 7829892A GB 7829892 A GB7829892 A GB 7829892A GB 2001172 A GB2001172 A GB 2001172A
Authority
GB
United Kingdom
Prior art keywords
analyte
receptor
enzyme
ligand
assay medium
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
GB7829892A
Other versions
GB2001172B (en
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Syva Co
Original Assignee
Syva Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority to US05/815,632 priority Critical patent/US4208479A/en
Priority to US05/815,487 priority patent/US4233401A/en
Application filed by Syva Co filed Critical Syva Co
Publication of GB2001172A publication Critical patent/GB2001172A/en
Application granted granted Critical
Publication of GB2001172B publication Critical patent/GB2001172B/en
Application status is Expired legal-status Critical

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07JSTEROIDS
    • C07J41/00Normal steroids containing one or more nitrogen atoms not belonging to a hetero ring
    • C07J41/0005Normal steroids containing one or more nitrogen atoms not belonging to a hetero ring the nitrogen atom being directly linked to the cyclopenta(a)hydro phenanthrene skeleton
    • C07J41/0016Oximes
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/536Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
    • G01N33/542Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching

Abstract

A method for competitive protein binding assays for determining a member of an immunological pair (ligand and receptor) whereby an enzyme conjugate to a member of the immunological pair is employed in combination with an enzyme inhibitor, conveniently an antibody to the enzyme. When ligand is the analyte, receptor for ligand is also included in the assay medium, while supplemental amounts of receptor need not be added when receptor is the analyte. The assay is carried out in an aqueous buffered medium, normally at constant temperature, by combining in the assay medium the unknown sample suspected of containing the analyte, enzyme-conjugate, ligand receptor (antiligand), enzyme inhibitor (antienzyme), and enzyme substrates, and the enzymatic activity in the assay medium determined. By comparing the observed enzymatic activity with an unknown to the enzymatic activity observed in an assay medium with a known amount of analyte, the amount of analyte can be quantitatively determined.
GB7829892A 1977-07-14 1978-07-14 Competitive protein binding assay Expired GB2001172B (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
US05/815,632 US4208479A (en) 1977-07-14 1977-07-14 Label modified immunoassays
US05/815,487 US4233401A (en) 1977-07-14 1977-07-14 Antienzyme homogeneous competitive binding assay

Publications (2)

Publication Number Publication Date
GB2001172A true GB2001172A (en) 1979-01-24
GB2001172B GB2001172B (en) 1982-01-27

Family

ID=27123960

Family Applications (1)

Application Number Title Priority Date Filing Date
GB7829892A Expired GB2001172B (en) 1977-07-14 1978-07-14 Competitive protein binding assay

Country Status (7)

Country Link
JP (1) JPS631544B2 (en)
AU (1) AU518002B2 (en)
CH (1) CH648414A5 (en)
DE (1) DE2830862C2 (en)
GB (1) GB2001172B (en)
IT (1) IT1105734B (en)
NL (1) NL7807607A (en)

Cited By (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2462709A1 (en) * 1979-07-26 1981-02-13 Syva Co Immunological assay method using enzymes, by load effect and composition for its implementation
EP0027036A1 (en) * 1979-10-03 1981-04-15 Colin Henry Self Assay method using enzymes as labeling substances
EP0034775A2 (en) * 1980-02-22 1981-09-02 Hans A. Dr. Thoma Homogeneous process for the competitive determination of ligands
FR2508486A1 (en) * 1981-06-30 1982-12-31 Wellcome Found Immuno-enzymatic determination method and kit for implementing it
EP0099567A2 (en) * 1982-07-19 1984-02-01 TECHNICON INSTRUMENTS CORPORATION(a Delaware corporation) Improved enzyme assay and method
EP0122833A1 (en) * 1983-03-15 1984-10-24 Institut Pasteur Heterogeneous assay method for antigenic biological substances using antibody-hybrid conjugates, and reagent therefor
EP0154276A2 (en) * 1984-03-09 1985-09-11 Miles Italiana SpA Specific binding assay employing anti-G6PDH as label
US4595655A (en) * 1979-10-03 1986-06-17 Self Colin H Assay method and reagent therefor
US4828981A (en) * 1983-08-24 1989-05-09 Synbiotics Corporation Immunoassays for determining Dirofilaria immitis infection using antiidiotype monoclonal antibody reagents
US4835099A (en) * 1986-11-20 1989-05-30 Becton, Dickinson And Company Signal enhancement in immunoassay by modulation of enzymatic catalysis
US5137804A (en) * 1988-05-10 1992-08-11 E. I. Du Pont De Nemours And Company Assay device and immunoassay
US5374524A (en) * 1988-05-10 1994-12-20 E. I. Du Pont De Nemours And Company Solution sandwich hybridization, capture and detection of amplified nucleic acids

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4273866A (en) * 1979-02-05 1981-06-16 Abbott Laboratories Ligand analog-irreversible enzyme inhibitor conjugates and methods for use
NZ199286A (en) * 1980-12-22 1986-05-09 Commw Serum Lab Commission A method of detecting antibodies or an antigenic or haptenic substance in a sample
DE3100061A1 (en) * 1981-01-02 1982-08-05 Hans A Dipl Chem Dr Thoma A process for the determination of ligands by competition reaction
DE3100062A1 (en) * 1981-01-02 1982-08-05 Hans A Dipl Chem Dr Thoma Homogeneous Method for the determination of ligand
JPS5888238U (en) * 1981-11-30 1983-06-15
JPS6364746B2 (en) * 1982-01-14 1988-12-13
IL67289A (en) * 1982-03-18 1985-12-31 Miles Lab Homogeneous immunoassay with labeled monoclonal anti-analyte
US4837395A (en) * 1985-05-10 1989-06-06 Syntex (U.S.A.) Inc. Single step heterogeneous assay
JPH0588784B2 (en) * 1986-04-22 1993-12-24 Konishiroku Photo Ind

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3817837A (en) * 1971-05-14 1974-06-18 Syva Corp Enzyme amplification assay

Cited By (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2462709A1 (en) * 1979-07-26 1981-02-13 Syva Co Immunological assay method using enzymes, by load effect and composition for its implementation
US4769321A (en) * 1979-10-03 1988-09-06 Self Colin H Assay method and reagent therefor
EP0027036A1 (en) * 1979-10-03 1981-04-15 Colin Henry Self Assay method using enzymes as labeling substances
WO1981001057A1 (en) * 1979-10-03 1981-04-16 C Self Assay method using enzymes as labelling substances
US4595655A (en) * 1979-10-03 1986-06-17 Self Colin H Assay method and reagent therefor
EP0034775A2 (en) * 1980-02-22 1981-09-02 Hans A. Dr. Thoma Homogeneous process for the competitive determination of ligands
EP0034775A3 (en) * 1980-02-22 1981-09-09 Hans A. Dr. Thoma Homogeneous process for the competitive determination of ligands
FR2508486A1 (en) * 1981-06-30 1982-12-31 Wellcome Found Immuno-enzymatic determination method and kit for implementing it
EP0099567A2 (en) * 1982-07-19 1984-02-01 TECHNICON INSTRUMENTS CORPORATION(a Delaware corporation) Improved enzyme assay and method
EP0099567A3 (en) * 1982-07-19 1984-05-30 Flow General, Inc. Improved enzyme assay and method
EP0122833A1 (en) * 1983-03-15 1984-10-24 Institut Pasteur Heterogeneous assay method for antigenic biological substances using antibody-hybrid conjugates, and reagent therefor
US4828981A (en) * 1983-08-24 1989-05-09 Synbiotics Corporation Immunoassays for determining Dirofilaria immitis infection using antiidiotype monoclonal antibody reagents
EP0154276A2 (en) * 1984-03-09 1985-09-11 Miles Italiana SpA Specific binding assay employing anti-G6PDH as label
EP0154276A3 (en) * 1984-03-09 1988-03-30 Miles Italiana Spa Specific binding assay employing anti-g6pdh as label
US4835099A (en) * 1986-11-20 1989-05-30 Becton, Dickinson And Company Signal enhancement in immunoassay by modulation of enzymatic catalysis
US5137804A (en) * 1988-05-10 1992-08-11 E. I. Du Pont De Nemours And Company Assay device and immunoassay
US5374524A (en) * 1988-05-10 1994-12-20 E. I. Du Pont De Nemours And Company Solution sandwich hybridization, capture and detection of amplified nucleic acids
US5391478A (en) * 1988-05-10 1995-02-21 E. I. Du Pont De Nemours And Company Assay device and immunoassay

Also Published As

Publication number Publication date
GB2001172B (en) 1982-01-27
DE2830862C2 (en) 1988-06-01
IT1105734B (en) 1985-11-04
AU3800278A (en) 1980-01-17
JPS631544B2 (en) 1988-01-13
AU518002B2 (en) 1981-09-10
NL7807607A (en) 1979-01-16
IT7850259D0 (en) 1978-07-12
JPS5420134A (en) 1979-02-15
CH648414A5 (en) 1985-03-15
DE2830862A1 (en) 1979-02-01

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Legal Events

Date Code Title Description
PE20 Patent expired after termination of 20 years

Effective date: 19980713