ES2571355T3 - Sistema de cultivo sin células alimentadoras ni xenocontaminantes para células madre embrionarias humanas - Google Patents

Sistema de cultivo sin células alimentadoras ni xenocontaminantes para células madre embrionarias humanas

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Publication number
ES2571355T3
ES2571355T3 ES03813286T ES03813286T ES2571355T3 ES 2571355 T3 ES2571355 T3 ES 2571355T3 ES 03813286 T ES03813286 T ES 03813286T ES 03813286 T ES03813286 T ES 03813286T ES 2571355 T3 ES2571355 T3 ES 2571355T3
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ES
Spain
Prior art keywords
xenocontaminants
culture system
embryonic stem
human embryonic
cells
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES03813286T
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English (en)
Inventor
Michal Amit
Joseph Itskovitz-Eldor
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Technion Research and Development Foundation Ltd
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Technion Research and Development Foundation Ltd
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Application filed by Technion Research and Development Foundation Ltd filed Critical Technion Research and Development Foundation Ltd
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0607—Non-embryonic pluripotent stem cells, e.g. MASC
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0603—Embryonic cells ; Embryoid bodies
    • C12N5/0606—Pluripotent embryonic cells, e.g. embryonic stem cells [ES]
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    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
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    • C12N5/0602—Vertebrate cells
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    • C12N2500/05—Inorganic components
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/20—Cytokines; Chemokines
    • C12N2501/23—Interleukins [IL]
    • C12N2501/235—Leukemia inhibitory factor [LIF]
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    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0696—Artificially induced pluripotent stem cells, e.g. iPS

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Biochemistry (AREA)
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  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Gynecology & Obstetrics (AREA)
  • Reproductive Health (AREA)
  • Hematology (AREA)
  • Immunology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

Un sistema de cultivo sin células alimentadoras ni xenocontaminantes que comprende una matriz y un medio de cultivo tisular que comprende sustitutivo del suero, TLFß1 y bFGF, pudiendo mantener el sistema de cultivo sin células alimentadoras ni xenocontaminantes, células madre embrionarias humanas cultivadas en su interior, en un estado proliferativo, pluripotente e indiferenciado.
ES03813286T 2002-12-16 2003-12-07 Sistema de cultivo sin células alimentadoras ni xenocontaminantes para células madre embrionarias humanas Expired - Lifetime ES2571355T3 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US43361902P 2002-12-16 2002-12-16
PCT/IL2003/001030 WO2004055155A2 (en) 2002-12-16 2003-12-07 Methods of preparing feeder cells-free, xeno-free human embryonic stem cells and stem cell cultures prepared using same

Publications (1)

Publication Number Publication Date
ES2571355T3 true ES2571355T3 (es) 2016-05-24

Family

ID=32595220

Family Applications (2)

Application Number Title Priority Date Filing Date
ES11190776T Expired - Lifetime ES2705683T3 (es) 2002-12-16 2003-12-07 Medio de cultivo de células madre pluripotentes
ES03813286T Expired - Lifetime ES2571355T3 (es) 2002-12-16 2003-12-07 Sistema de cultivo sin células alimentadoras ni xenocontaminantes para células madre embrionarias humanas

Family Applications Before (1)

Application Number Title Priority Date Filing Date
ES11190776T Expired - Lifetime ES2705683T3 (es) 2002-12-16 2003-12-07 Medio de cultivo de células madre pluripotentes

Country Status (14)

Country Link
US (8) US7592175B2 (es)
EP (2) EP2457999B1 (es)
JP (1) JP4613069B2 (es)
KR (1) KR101156745B1 (es)
CN (1) CN100549163C (es)
AU (2) AU2003303071B2 (es)
CA (1) CA2508880C (es)
DK (2) DK2457999T3 (es)
ES (2) ES2705683T3 (es)
HU (1) HUE028026T2 (es)
IL (2) IL168984A (es)
PT (2) PT1572984E (es)
SI (1) SI1572984T1 (es)
WO (1) WO2004055155A2 (es)

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CN101878298B (zh) 2007-11-27 2017-08-15 生命扫描有限公司 人胚胎干细胞的分化
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