CN1856576A - 腺伴随病毒(aav)进化支、序列、含有这些序列的载体及它们的应用 - Google Patents
腺伴随病毒(aav)进化支、序列、含有这些序列的载体及它们的应用 Download PDFInfo
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Abstract
本发明提供了新的腺伴随病毒衣壳与载体的序列以及含有这些序列的宿主细胞。也描述了使用这种宿主细胞和载体产生rAAV颗粒的方法。也提供了使用本发明的载体AAV-介导的递送治疗性和免疫原性基因。
Description
关于联邦政府资助的研究或开发的声明
本申请包括由国家卫生研究院(NIH)的NIDDK P30 DK47757和NHLBI P01HL59407拨款资助的工作。美国政府享有本发明的一定权力。
发明背景
腺伴随病毒(AAV)是细小病毒科家族的成员,它是小的无包膜、二十面体病毒,具有4.7千碱基(kb)到6kb的单链线形DNA基因组。由于该病毒是作为纯化的腺病毒原种的污染物被发现,AAV指定为依赖病毒属(Dependovirus)。AAV的生命周期包括潜伏期(AAV基因组在感染后位点特异性地整合进宿主染色体),感染期(腺病毒或单纯疱疹病毒感染后,整合的基因组接着得到拯救、复制并包装进感染性病毒)。非致病性的特性、感染性的宽宿主范围(包括非分裂细胞)与潜在的位点特异性染色体整合使得AAV成为用于基因转移的有吸引力的工具。
近来的研究表明AAV载体是用于基因递送的优选运载体。迄今为止,已从人或非人灵长类(NHP)分离了几种表征良好的不同AAV。
已发现不同血清型的AAV表现出不同的转染效率,也表现出对不同细胞或组织的向性。然而,以前还未对这些不同血清型之间的关系进行研究。
基于AAV的构建物用于递送异源分子是理想的。
发明概述
本发明提供AAV的系统发生相关序列的“超家族”或“进化支”。这些AAV进化支提供用于将分子靶向和/或递送至所需靶细胞或组织的AAV序列源。
本发明一方面提供具有至少三个AAV成员的AAV进化支,这些成员是基于AAV vp1氨基酸序列对比,使用自引值为至少75%(基于至少1000次复制)的邻接启发式算法(Neighbor-Joining heuristic)与不大于0.05的泊松校正距离测量值(Poisson correction distance measurement)确定为系统发生相关的。合适的AAV进化支由用于产生载体的AAV序列组成。
本发明还提供以前未知的人AAV血清型,这些血清型在本文中称为克隆28.4/hu.14或AAV血清型9。因此,本发明另一方面提供一种由AAV衣壳组成的血清型9的AAV,该衣壳在血清学上与SEQ ID NO:123的氨基酸1-736的序列的衣壳相关,在血清学上与AAV1、AAV2、AAV3、AAV4、AAV5、AAV6、AAV7或AAV8中的任一衣壳蛋白不同。
用该huAAV9的衣壳构建的载体表现出的基因转移效率类似于肝脏中的AAV8、优于肌肉中的AAV并且比肺中的AAV5高200倍。此外,该新的人AAV血清型与以前所述的AAV1到AAV8的序列同一性小于85%,并且不被任何这些AAV交叉中和。
本发明也提供其它新的AAV序列、含有这些序列的组合物及它们的应用。有利的是,这些组合物特别适用于出于治疗或预防目的需要反复施用的AAV载体。
本发明的这些和其它方面可通过以下发明详述得到明白。
附图简述
图1是使用带泊松校正距离测量值的邻接启发式算法构建的系统发生关系树。基于分离的AAV vp1衣壳蛋白与分组于进化支中分离的AAV确定关系。单个衣壳克隆的组基于它们共同的祖先分类在进化支中。进化支以A到F命名;亚型以进化支字母加上一个数值表示。
图2A-2AE对比了本发明的AAV vp1衣壳蛋白的氨基酸序列(公布了各个序列的编号)与以前公布的AAV1[SEQ ID NO:219]、AAV2[SEQ ID NO:221]、AAV3-3[SEQ ID NO:217]、AAV4-4[SEQ ID NO:218]、AAV5[SEQ ID NO:216]、AAV6[SEQ ID NO:220]、AAV7[SEQ ID NO:222]、AAV8[SEQ ID NO:223]与rh.25/42-15、29.3/bb.1、cy.2、29.5/bb.2、rh.32、rh.33、rh.34、rh.10、rh.24、rh.14、rh.16、rh.17、rh.12、rh.18、rh.21(以前称为41.10)、rh.25(以前称为41.15)、rh2、rh.31、cy.3、cy.5、rh.13、cy.4、cy.6、rh.22、rh.19、rh.35、rh.37、rh.36、rh.23、rh.8和ch5[公开的美国专利申请号2003/0138772 A1(2003年7月24日)]。本发明的序列包括hu.14/AAV9[SEQ ID NO:123]、hu.17[SEQ ID NO:83]、hu.6[SEQ ID NO:84]、hu.42[SEQ ID NO:85]、hu.38[SEQ ID NO:86]、hu.40[SEQ ID NO:87]、hu.37[SEQ IDNO:88]、rh.40[SEQ ID NO:92]、rh.52[SEQ ID NO:96]、rh.53[SEQ ID NO:97]、rh.49[SEQ ID NO:103]、rh.51[SEQ ID NO:104]、rh.57[SEQ ID NO:105]、rh.58[SEQID NO:106]、rh.61[SEQ ID NO:107]、rh.50[SEQ ID NO:108]、rh.43[SEQ ID NO:163]、rh.62[SEQ ID NO:114]、rh.48[SEQ ID NO:115]、4-9/rh.54[SEQ ID NO:116]和4-19/rh.55[SEQ ID NO:117]、hu.31[SEQ ID NO:121]、hu.32[SEQ ID NO:122]、hu.34[SEQ ID NO:125]、hu.45[SEQ ID NO:127]、hu.47[SEQ ID NO:128]、hu.13[SEQID NO:129]、hu.28[SEQ ID NO:130]、hu.29[SEQ ID NO:132]、hu.19[SEQ IDNO:133]、hu.20[SEQ ID NO:134]、hu.21[SEQ ID NO:135]、hu.23.2[SEQ ID NO:137]、hu.22[SEQ ID NO:138]、hu.27[SEQ ID NO:140]、hu.4[SEQ ID NO:141]、hu.2[SEQ IDNO:143]、hu.1[SEQ ID NO:144]、hu.3[SEQ ID NO:145]、hu.25[SEQ ID NO:146]、hu.15[SEQ ID NO:147]、hu.16[SEQ ID NO:148]、hu.18[SEQ ID NO:149]、hu.7[SEQID NO:150]、hu.11[SEQ ID NO:153]、hu.9[SEQ ID NO:155]、hu.10[SEQ ID NO:156]、hu.48[SEQ ID NO:157]、hu.44[SEQ ID NO:158]、hu.46[SEQ ID NO:159]、hu.43[SEQID NO:160]、hu.35[SEQ ID NO:164]、hu.24[SEQ ID NO:136]、rh.64[SEQ ID NO:99]、hu.41[SEQ ID NO:91]、hu.39[SEQ ID NO:102]、hu.67[SEQ ID NO:198]、hu.66[SEQID NO:197]、hu.51[SEQ ID NO:190]、hu.52[SEQ ID NO:191]、hu.49[SEQ IDNO:189]、hu.59[SEQ ID NO:192]、hu.57[SEQ ID NO:193]、hu.58[SEQ ID NO:194]、hu.63[SEQ ID NO:195]、hu.64[SEQ ID NO:196]、hu.60[SEQ ID NO:184]、hu.61[SEQID NO:185]、hu.53[SEQ ID NO:186]、hu.55[SEQ ID NO:187]、hu.54[SEQ ID NO:88]、hu.6[SEQ ID NO:84]与rh.56[SEQ ID NO:152]。这些衣壳序列也见引为参考的序列表中。
图3A-3CN对比了本发明的AAV vp1衣壳蛋白的核酸序列(公布了各个序列的编号)与以前公布的AAV5(SEQ ID NO:199)、AAV3-3(SEQ ID NO:200)、AAV4-4(SEQ ID NO:201)、AAV1(SEQ ID NO:202)、AAV6(SEQ ID NO:203)、AAV2(SEQ ID NO:211)、AAV7(SEQ ID NO:213)与AAV8(SEQ ID NO:214)、rh.25/42-15、29.3/bb.1、cy.2、29.5/bb.2、rh.32、rh.33、rh.34、rh.10、rh.24、rh.14、rh.16、rh.17、rh.12、rh.18、rh.21(以前称为41.10)、rh.25(以前称为41.15;GenBank登录号AY530557)、rh2、rh.31、cy.3、cy.5、rh.13、cy.4、cy.6、rh.22、rh.19、rh.35、rh.36、rh.37、rh.23、rh.8与ch.5[公开的美国专利申请号2003/0138772 A1(2003年7月24日)]。本发明的核酸序列包括hu.14/AAV9(SEQ ID NO:3)、LG-4/rh.38(SEQ IDNO:7)、LG-10/rh.40(SEQID NO:14)、N721-8/rh.43(SEQ ID NO:43)、1-8/rh.49(SEQ IDNO:25)、2-4/rh.50(SEQ ID NO:23)、2-5/rh.51(SEQ ID NO:22)、3-9/rh.52(SEQ IDNO:18)、3-11/rh.53(SEQ ID NO:17)、5-3/rh.57(SEQ ID NO:26)、5-22/rh.58(SEQ IDNO:27)、2-3/rh.61(SEQ ID NO:21)、4-8/rh.64(SEQ ID NO:15)、3.1/hu.6(SEQ IDNO:5)、33.12/hu.17(SEQ ID NO:4)、106.1/hu.37(SEQ ID NO:10)、LG-9/hu.39(SEQ IDNO:24)、114.3/hu.40(SEQ ID NO:11)、127.2/hu.41(SEQ ID NO:6)、127.5/hu.42(SEQID NO:8)与hu.66(SEQ ID NO:173)、2-15/rh.62(SEQ ID NO:33)、1-7/rh.48(SEQ IDNO:32)、4-9/rh.54(SEQ ID NO:40)、4-19/rh.55(SEQ ID NO:37)、52/hu.19(SEQ IDNO:62)、52.1/hu.20(SEQ ID NO:63)、54.5/hu.23(SEQ ID NO:60)、54.2/hu.22(SEQ IDNO:67)、54.7/hu.24(SEQ ID NO:66)、54.1/hu.21(SEQ ID NO:65)、54.4R/hu.27(SEQ IDNO:64)、46.2/hu.28(SEQ ID NO:68)、46.6/hu.29(SEQ ID NO:69)、128.1/hu.43(SEQ IDNO:80)、128.3/hu.44(SEQ ID NO:81)与130.4/hu.48(SEQ ID NO:78)、3.1/hu.9(SEQ IDNO:58)、16.8/hu.10(SEQ ID NO:56)、16.12/hu.11(SEQ ID NO:57)、145.1/hu.53(SEQID NO:176)、145.6/hu.55(SEQ ID NO:178)、145.5/hu.54(SEQ ID NO:177)、7.3/hu.7(SEQ ID NO:55)、52/hu.19(SEQ ID NO:62)、33.4/hu.15(SEQ ID NO:50)、33.8/hu.16(SEQ ID NO:51)、58.2/hu.25(SEQ ID NO:49)、161.10/hu.60(SEQ IDNO:170)、H-5/hu.3(SEQ ID NO:44)、H-1/hu.1(SEQ ID NO:46)、161.6/hu.61(SEQ IDNO:174)、hu.31(SEQ ID NO:1)、hu.32(SEQ ID NO:2)、hu.46(SEQ ID NO:82)、hu.34(SEQ ID NO:72)、hu.47(SEQ ID NO:77)、hu.63(SEQ ID NO:204)、hu.56(SEQ IDNO:205)、hu.45(SEQ ID NO:76)、hu.57(SEQ ID NO:206)、hu.35(SEQ ID NO:73)、hu.58(SEQ ID NO:207)、hu.51(SEQ ID NO:208)、hu.49(SEQ ID NO:209)、hu.52(SEQID NO:210)、hu.13(SEQ ID NO:71)、hu.64(SEQ ID NO:212)、rh.56(SEQ ID NO:54)、hu.2(SEQ ID NO:48)、hu.18(SEQ ID NO:52)、hu.4(SEQ ID NO:47)与hu.67(SEQ IDNO:215)。这些衣壳序列也见引为参考的序列表中。
图4A-4D提供了对基于AAV的新灵长类载体在体外和体内基因转移效率的评价。AAV载体是如[Gao等,Proc Natl Acad Sci美国,99:11854-11859(2002年,9月3日)]所述具有AAV1、2、5、7、8和6与ch.5、rh.34、cy.5、rh.20、rh.8和AAV9衣壳的假型。如图4A所示,为进行体外研究,接种于95-孔板的84-32细胞(表达腺病毒血清型E4的293细胞)以每细胞1×104GC的感染复数(MoI)用假型AAVCMVEGFP载体感染。感染后48小时使用紫外显微镜将相对EGFP转导效率估计为绿色细胞的百分比并显示在Y轴上。为进行体内研究,分别经门内(intraportal)(图4B)、气管内(图4C)和肌肉内(图4D)以每只动物1×1011GC的剂量给予NCR裸鼠(4-6周龄)的肝(图4B)、肺(图4C)和肌肉(图4D)以表达分泌的报道基因A1AT的载体。在基因转移后第28天比较血清A1AT水平(ng/ml)并示于Y轴上。X轴表示所分析的AAV与它们所属的进化支。
发明详述
在任何用于治疗或预防的载体库中,为所需应用选择载体源,需要能将大分子携带至靶细胞的各种不同载体。迄今为止,关于将AAV用作载体的一个问题是缺乏各种不同的病毒来源。本发明通过提供AAV的进化支来克服该问题,所述进化支用于选择系统发生相关的(或选择治疗方案所需的),或系统发生不同的AAV并用于预测功能。本发明还提供新的AAV病毒。
当术语“基本同源性”或“基本相似性”指核酸或其片段时意味着当合适的核苷酸插入物或缺失物与另一核酸(或其互补链)最佳对比时,对比序列中至少约95%到99%的核苷酸序列同一性。优选在全长序列、或其开放读框、或另一至少长15个核苷酸的合适片段上具有同源性。合适片段的例子描述于本文。
文中用于核酸序列的术语“序列同一性”、“序列同一性百分比”或“相同百分比”指当对比最大一致性时,两条序列中的残基相同。序列同一性比较的长度可是基因组全长、基因编码序列的的全长,或需要至少约500到5000个核苷酸的片段。然而,较小片段中的同一性也是需要的,例如至少约9个核苷酸、通常至少约20到24个核苷酸、至少约28到32个核苷酸、至少约36或更多个核苷酸的片段。类似地,氨基酸序列、蛋白全长、或其片段的“序列同一性百分比”可容易地确定。合适片段的长度至少约8个氨基酸,也可达约700个氨基酸。合适片段的例子描述于文中。
当术语“基本同源性”或“基本相似性”指氨基酸或其片段时意味着当合适的氨基酸插入物或缺失物与另一氨基酸(或其互补链)最佳对比时,对比序列中至少约95%到99%的氨基酸序列同一性。优选在全长序列,或其蛋白质(例如,cap蛋白、rep蛋白),或其至少8个氨基酸,或更希望至少15个氨基酸长度的片段上具有同源性。合适片段的例子描述于本文。
术语“高度保守的”表示至少80%的同一性、优选至少90%的同一性、更优选超过97%的同一性。本领域的技术人员可凭借本领域技术人员已知的算法和计算机程序容易地确定同一性。
一般当提及在两种不同的腺伴随病毒之间的“同一性”、“同源性”或“相似性”时,“同一性”、“同源性”或“相似性”是参考“对比”的序列确定的。“对比”的序列或“对比”指多核酸序列或蛋白(氨基酸)序列,与参考序列相比,该序列通常含有缺失的或附加的碱基或氨基酸的校正。在实施例中,使用公开的AAV2或AAV1序列作为参考点进行AAV对比。然而,本领域的技术人员可容易地选择另一AAV序列作为参考。
可使用任何各种公用或市售可得的多序列对比程序进行对比。这种程序的例子包括通过因特网的万维服务器可得的“Clustal W”、“CAP序列组件”、“MAP”和“MEME”。这种程序的其它来源是本领域技术人员已知的。或者也可使用VectorNTI实用程序。许多本领域已知的算法,包括包含在上述程序中的算法也可用于测量核苷酸序列同一性。使用GCG6.1版中的程序FastaTM来比较多核苷酸序列是另一个例子。FastaTM提供在查询和检索序列之间最佳重叠区域的对比和序列同一性百分比。例如,核酸序列之间的序列同一性百分比可使用GCG6.1版提供的具有默认参数的FastaTM(字长为6和用于计分矩阵的NOPAM系数)来确定,该程序纳入本文引作参考。多序列对比程序也可应用于氨基酸序列,例如“Clustal X”、“MAP”、“PIMA”、“MSA”、“BLOCKMAKER”、“MEME”和“Match-Box”程序。虽然本领域的技术人员可视需要改变这些设置,但通常这些程序以默认设置使用。或者,本领域的技术人员可利用另一种算法或计算机程序,该算法或程序提供的同一性或算法的水平至少同参考的算法和程序提供的一样。参见,例如J.D.Thomson等,Nucl.Acids.Res.,“多序列对比的综合比较”(A comprehensive comparison ofmultiple sequence alignments),27(13):2682-2690(1999)。
术语“血清型”区分具有在血清学上不同于其它AAV血清型的一种衣壳的AAV。血清学特殊性是基于与其它AAV相比,针对AAV的抗体之间缺乏交叉反应性来确定。
交叉反应性通常在中和抗体试验中测定。就该试验而言,使用腺伴随病毒在家兔或其它合适的动物模型中产生抗特异性AAV的多克隆血清。然后在该试验中测试所产生的抗特异性AAV的血清中和相同(同源)或异源AAV的能力。达到50%中和作用的稀释度看作中和抗体滴度。如果就两种AAV而言,倒数形式的异源滴度除以同源滴度的商小于16,则此两种AAV可视为是相同的血清型。相反,如果倒数形式的异源滴度与同源滴度之比是16或更大,此两种AAV视为是不同血清型。
如本文所定义,为形成血清型9,所产生针对所选AAV衣壳的抗体必须与AAV1、AAV2、AAV3、AAV4、AAV5、AAV6、AAV7或AAV8中任一种不交叉反应。在一个实施方案中,本发明提供一种在本文中鉴定为人AAV血清型9的新血清型的AAV衣壳。
本说明书与权利要求书使用的术语“含有”和“包括”是包括其它成分、元素、整数、步骤等在内。相反,术语“由...组成”和其变体不包括其它成分、元素、整数、步骤等在内。
1.进化支
在一方面,本发明提供了AAV的进化支。进化支是基于AAV vp1氨基酸序列对比,使用自引值为至少75%(至少1000次复制)的邻接启发式算法与不大于0.05的泊松校正距离测量值确定为互相系统发生相关的一组AAV。
邻接算法详细描述于参考文献。参见,例如M.Nei与S.Kumar,《分子进化与系统发生学》(Molecular Evolution and Phylogenetics)(牛津大学出版社,纽约(2000)。计算机程序可用于执行该算法。例如,MEGA v2.1程序执行改进的Nei-Gojobori方法。使用这些技术与计算机程序,AAV vp1衣壳蛋白的序列,本领域的技术人员可容易地确定所选择的AAV是否包含在文中所鉴定的一种进化支内、另一种进化支内、或在这些进化支之外。
虽然本文所定义的进化支主要基于天然的AAV vp1衣壳,这些进化支不限于天然的AAV。这些进化支可包括非天然AAV,包括(但不限于)重组、修饰、嵌合、杂交、合成、人工的等,基于AAV vp1氨基酸序列对比,使用自引值为至少75%(至少1000次复制)的邻接启发式算法与不大于0.05的泊松校正距离测量值确定为系统发生相关的AAV。
本文所述的进化支包括进化支A(以AAV1和AAV6表示)、进化支B(以AAV2表示)、进化支C(以AAV2-AAV3杂交体表示)、进化支D(以AAV7表示)、进化支E(以AAV8表示)与进化支F(以人AAV9表示)。这些进化支以前文所述AAV血清型的进化支的成员代表。前文所述的AAV1和AAV6是单一进化支(进化支A)的成员,该进化支中4种分离物回收自3种人。前文所述AAV3和AAV5血清型明显相互不同,但在本文所述的筛选中未检测到,并且未包括于任何这些进化支中。
进化支B(AAV2)和进化支C(AAV2-AAV3杂交体)是在人中发现的AAV最丰富的(AAV2有22种分离物来自12种个体,进化支C有17个分离物来自8种个体)。
大量的序列分组于进化支D(AAV7)或进化支E(AAV8)。有趣的是,这些进化支普遍存在于不同物种中。进化支D对恒河猴与南美弥猴是独特的,具有分离自10种不同动物的15个成员。由于进化支E在人和非人灵长类中发现,它是令人感兴趣的:9种分离物回收自7种人而21种分离物得自9种不同的非人灵长类,包括恒河猴、狒狒和豚尾弥猴(pigtail monkey)。
在其它两种动物中证实了某些序列的杂交特性,虽然在该情况中所有的序列似乎具有起源于两种协同感染病毒(在两种动物中是进化支D与进化支E病毒)的独立而不同的重组。这些重组体无一在其它动物或受试对象中鉴定到。
因为进化支C(AAV2-AAV3杂交体)在6种不同的人对象中鉴定到,重组甚至导致了合适的后代。另一方面,就AAV7-AAV8杂交体而言,仅有少数重组情况涉及AAV进化。这些重组情况说明AAV能重组,从而产生框内基因以及在一些情况中产生可包装和/或感染性衣壳结构。另一方面,进化支C(AAV2-AAV3杂交体进化支)是一组通过重组获得选择性优点的病毒,这种重组使它们忍受环境压力。
A.进化支A(以AAV1和AAV6表示):
在另一方面,本发明提供进化支A,其特征在于含有前文公开的AAV1和AAV6。参见,例如2000年5月18日的国际公开号WO 00/28061;Rutledge等,JVirol,72(1):309-319(1998年1月)。此外,该进化支包含有新AAV,包括(不限于)128.1/hu.43[SEQ ID NO:80和160]、128.3/hu.44[SEQ ID NO:81和158]、130.4/hu.48[SEQ ID NO:78和157]与hu.46[SEQ ID NO:82和159]。本发明还提供修饰的hu.43衣壳[SEQ ID NO:236]与修饰的hu.46衣壳[SEQ ID NO:224]。
在一个实施方案中,该进化支的一个或多个成员的衣壳与AAV1和/或AAV6衣壳的全长vp1、vp2或vp3具有至少85%、至少90%、至少95%或至少97%的氨基酸同一性。
在另一个实施方案中,本发明提供进化支A的新AAV,前提是新AAV均不含有AAV1或AAV6的任一种衣壳。这些AAV可包括(不限于)具有衍生自以下一种或多种衣壳的AAV:128.1/hu.43[SEQ ID NO:80和160]、修饰的hu.43[SEQ IDNO:236]、128.3/hu.44[SEQ ID NO:81和158]、hu.46[SEQ ID NO:82和159]、修饰的hu.46[SEQ ID NO:224]与130.4/hu.48[SEQ ID NO:78和157]。
B.进化支B(AAV2进化支):
在另一个实施方案中,本发明提供进化支B。
该进化支的特征在于最少包含前文所述的AAV2与本发明的新AAV,新AAV包括52/hu.19[SEQ ID NO:62和133]、52.1/hu.20[SEQ ID NO:63和134]、54.5/hu.23[SEQ ID NO:60和137]、54.2/hu.22[SEQ ID NO:67和138]、54.7/hu.24[SEQID NO:66和136]、54.1/hu.21[SEQ ID NO:65和135]、54.4R/hu.27[SEQ ID NO:64和140]、46.2/hu.28[SEQ ID NO:68和130]、46.6/hu.29[SEQ ID NO:69和132]、修饰的hu.29[SEQ ID NO:225]、172.1/hu.63[SEQ ID NO:171和195;GenBank登录号AY530624]、172.2/hu.64[SEQ ID NO:172和196;GenBank登录号AY530625]、24.5/hu.13[SEQ ID NO:71和129;GenBank登录号AY530578]、145.6/hu.56[SEQ IDNO:168和192]、hu.57[SEQ ID NO:169和193]、136.1/hu.49[SEQ ID NO:165和189]、156.1/hu.58[SEQ ID NO:179和194]、72.2/hu.34[SEQ ID NO:72和125;GenBank登录号AY530598]、72.3/hu.35[SEQ ID NO:73和164;GenBank登录号AY530599]、130.1/hu.47[SEQ ID NO:77和128]、129.1/hu.45(SEQ ID NO:76和127;GenBank登录号AY530608)、140.1/hu.51[SEQ ID NO:161和190;GenBank登录号AY530613]和140.2/hu.52[SEQ ID NO:167和191;GenBank登录号AY530614]。
在一个实施方案中,该进化支的一个或多个成员的衣壳与AAV2衣壳的全长vp1、vp2或vp3具有至少85%、至少90%、至少95%或至少97%的氨基酸同一性。
在另一个实施方案中,本发明提供进化支B的新AAV,前提是新AAV均不含有AAV2衣壳。这些AAV可包括(不限于)具有衍生自以下一种或多种衣壳的AAV:52/hu.19[SEQ ID NO:62和133]、52.1/hu.20[SEQ ID NO:63和134]、54.5/hu.23[SEQ ID NO:60和137]、54.2/hu.22[SEQ ID NO:67和138]、54.7/hu.24[SEQID NO:66和136]、54.1/hu.21[SEQ ID NO:65和135]、54.4R/hu.27[SEQ ID NO:64和140];46.2/hu.28[SEQ ID NO:68和130];46.6/hu.29[SEQ ID NO:69和132];修饰的hu.29[SEQ ID NO:225]、172.1/hu.63[SEQ ID NO:171和195]、172.2/hu.64[SEQID NO:172和196];24.5/hu.13[SEQ ID NO:71和129]、145.6/hu.56[SEQ ID NO:168和192;GenBank登录号AY530618]、hu.57[SEQ ID NO:169和193;GenBank登录号AY530619]、136.1/hu.49[SEQ ID NO:165和189;GenBank登录号AY530612]、156.1/hu.58[SEQ ID NO:179和194;GenBank登录号AY530620]、72.2/hu.34[SEQ IDNO:72和125]、72.3/hu.35[SEQ ID NO:73和164]、129.1/hu.45[SEQ ID NO:76和127]、130.1/hu.47[SEQ ID NO:77和128、GenBank登录号AY530610]、140.1/hu.51[SEQ IDNO:161和190;GenBank登录号AY530613]和140.2/hu.52[SEQ ID NO:167和191;GenBank登录号AY530614]。
C.进化支C(AAV2-AAV3杂交体进化支)
另一个方面,本发明提供进化支C,该进化支的特征在于包含前文所公开的AAV2与AAV3的杂交体的AAV,例如H-6/hu.4、H-2/hu.2[美国专利申请2003/0138772(2003年6月24日)]。此外,该进化支含有新AAV,包括(不限于)3.1/hu.9[SEQ ID NO:58和155]、16.8/hu.10[SEQ ID NO:56和156]、16.12/hu.11[SEQ ID NO:57和153]、145.1/hu.53[SEQ ID NO:176和186]、145.6/hu.55[SEQ ID NO:178和187]、145.5/hu.54[SEQ ID NO:177和188]、7.3/hu.7[SEQ ID NO:55和150;现在以GenBank登录号AY530628保藏]、修饰的hu.7[SEQ ID NO:226]、33.4/hu.15[SEQ ID NO:50和147]、33.8/hu.16[SEQ ID NO:51和148]、hu.18[SEQ ID NO:52和149]、58.2/hu.25[SEQ ID NO:49和146]、161.10/hu.60[SEQ ID NO:170和184]、H-5/hu.3[SEQ ID NO:44和145]、H-1/hu.1[SEQID NO:46和144]和161.6/hu.61[SEQ ID NO:174和185]。
在一个实施方案中,该进化支的一个或多个成员的衣壳与hu.4和/或hu.2衣壳的全长vp1、vp2或vp3具有至少85%、至少90%、至少95%或至少97%的氨基酸同一性。
在另一个实施方案中,本发明提供进化支C(AAV2-AAV3杂交体进化支)的新AAV,前提是该新AAV均不含有hu.2或hu.4的衣壳。这些AAV可包括(不限于)具有衍生自以下一种或多种衣壳的AAV:3.1/hu.9[SEQ ID NO:58和155]、16.8/hu.10[SEQ ID NO:56和156]、16.12/hu.11[SEQ ID NO:57和153]、145.1/hu.53[SEQ ID NO:176和186]、145.6/hu.55[SEQ ID NO:178和187]、145.5/hu.54[SEQ ID NO:177和188]、7.3/hu.7[SEQ ID NO:55和150]、修饰的hu.7[SEQ ID NO:226]、33.4/hu.15[SEQ ID NO:50和147]、33.8/hu.16[SEQ ID NO:51和148]、58.2/hu.25[SEQ ID NO:49和146]、161.10/hu.60[SEQ ID NO:170和184]、H-5/hu.3[SEQ ID NO:44和145]、H-1/hu.1[SEQ ID NO:46和144]和161.6/hu.61[SEQID NO:174和185]。
D.进化支D(AAV7进化支)
在另一个实施方案中,本发明提供进化支D。该进化支的特征在于包含前文所述的AAV7[G.Gao等,Proc.Natl.Acad.Sci美国,99:11854-9(2002年9月3日)]。编码该AAV7衣壳的核酸序列见SEQ ID NO:184;AAV7衣壳的氨基酸序列见SEQID NO:185。此外,该进化支含有许多前文所述的AAV序列,包括cy.2、cy.3、cy.4、cy.5、cy.6、rh.13、rh.37、rh.36和rh.35[公开的美国专利申请号US2003/0138772A1(2003年7月24日)]。另外,AAV7进化支含有新AAV序列,包括(不限于)2-15/rh.62[SEQ ID NO:33和114]、1-7/rh.48[SEQ ID NO:32和115]、4-9/rh.54[SEQID NO:40和116]与4-19/rh.55[SEQ ID NO:37和117]。本发明还包括修饰的cy.5[SEQ ID NO:227]、修饰的rh.13[SEQ ID NO:228]与修饰的rh.37[SEQ IDNO:229]。
在一个实施方案中,该进化支的一个或多个成员的衣壳与AAV7衣壳,SEQ IDNO:184和185的全长vp1、vp2或vp3具有至少85%、至少90%、至少95%或至少97%的氨基酸同一性。
在另一个实施方案中,本发明提供进化支D的新AAV,前提是该新AAV均不含有cy.2、cy.3、cy.4、cy.5、cy.6、rh.13、rh.37、rh.36和rh.35的任一种衣壳。这些AAV可包括(不限于)具有衍生自以下一种或多种衣壳的AAV:2-15/rh.62[SEQID NO:33和114]、1-7/rh.48[SEQ ID NO:32和115]、4-9/rh.54[SEQ ID NO:40和116]与4-19/rh.55[SEQ ID NO:37和117]。
E.进化支E(AAV8进化支)
一方面,本发明提供进化支E。该进化支的特征在于包含前文所述的AAV8[G.Gao等,Proc.Natl.Acad.Sci美国,99:11854-9(2002年9月3日)]、43.1/rh.2、44.2/rh.10、rh.25、29.3/bb.1与29.5/bb.2(公开的美国专利申请号US 2003/0138772A1(2003年7月24日))。
此外,该进化支的新AAV序列包括(不限于)例如30.10/pi.1[SEQ ID NO:28和93]、30.12/pi.2[SEQ ID NO:30和95]、30.19/pi.3[SEQ ID NO:29和94]、LG-4/rh.38[SEQ ID NO:7和86]、LG-10/rh.40[SEQ ID NO:14和92]、N721-8/rh.43[SEQ ID NO:43和163]、1-8/rh.49[SEQ ID NO:25和103]、2-4/rh.50[SEQID NO:23和108]、2-5/rh.51[SEQ ID NO:22和104]、3-9/rh.52[SEQ ID NO:18和96]、3-11/rh.53[SEQ ID NO:17和97]、5-3/rh.57[SEQ ID NO:26和105]、5-22/rh.58[SEQ IDNO:27和58]、2-3/rh.61[SEQ ID NO:21和107]、4-8/rh.64[SEQ ID NO:15和99]、3.1/hu.6[SEQ ID NO:5和84]、33.12/hu.17[SEQ ID NO:4和83]、106.1/hu.37[SEQ IDNO:10和88]、LG-9/hu.39[SEQ ID NO:24和102]、114.3/hu.40[SEQ ID NO:11和87]、127.2/hu.41[SEQ ID NO:6和91]、127.5/hu.42[SEQ ID NO:8和85]、hu.66[SEQ IDNO:173和197]和hu.67[SEQ ID NO:174和198]。该进化支还包括修饰的rh.2[SEQ IDNO:231]、修饰的rh.58[SEQ ID NO:232]、修饰的rh.64[SEQ ID NO:233]。
在一个实施方案中,该进化支的一个或多个成员的衣壳与AAV8衣壳的全长vp1、vp2或vp3具有至少85%、至少90%、至少95%或至少97%的氨基酸同一性。编码该AAV8衣壳的核酸序列见SEQ ID NO:186;该衣壳的氨基酸序列见SEQ IDNO:187。
在另一个实施方案中,本发明提供进化支E的新AAV,前提是该新AAV均不含有AAV8.rh.8、44.2/rh.10、rh.25、29.3/bb.1与29.5/bb.2[公开的美国专利申请号US2003/0138772 A1(2003年7月24日)]的任一种衣壳。这些AAV可包括(不限于)具有衍生自以下一种或多种衣壳的AAV:30.10/pi.1[SEQ ID NO:28和93]、30.12/pi.2[SEQ ID NO:30和95]、30.19/pi.3[SEQ ID NO:29和94]、LG-4/rh.38[SEQ IDNO:7和86]、LG-1O/rh.40[SEQ ID NO:14和92]、N721-8/rh.43[SEQ ID NO:43和163]、1-8/rh.49[SEQ ID NO:25和103]、2-4/rh.50[SEQ ID NO:23和108]、2-5/rh.51[SEQ IDNO:22和104]、3-9/rh.52[SEQ ID NO:18和96]、3-11/rh.53[SEQ ID NO:17和97]、5-3/rh.57[SEQ ID NO:26和105]、5-22/rh.58[SEQ ID NO:27和58]、修饰的rh.58[SEQID NO:232]、2-3/rh.61[SEQ ID NO:21和107]、4-8/rh.64[SEQ ID NO:15和99]、修饰的rh.64[SEQ ID NO:233]、3.1/hu.6[SEQ ID NO:5和84]、33.12/hu.17[SEQ ID NO:4和83]、106.1/hu.37[SEQ ID NO:10和88]、LG-9/hu.39[SEQ ID NO:24和102]、114.3/hu.40[SEQ ID NO:11和87]、127.2/hu.41[SEQ ID NO:6和91]、127.5/hu.42[SEQID NO:8和85]、hu.66[SEQ ID NO:173和197]和hu.67[SEQ ID NO:174和198]。
F.进化支(AAV9进化支)
本文将该进化支鉴定为名为hu.14/AAV9[SEQ ID NO:3和123]的新AAV血清型。此外,该进化支含有其它的新序列,包括hu.31[SEQ ID NO:1和121]与hu.32[SEQ ID NO:2和122]。
在一个实施方案中,该进化支的一个或多个成员的衣壳与AAV9衣壳,SEQ IDNO:3和123的全长vp1、vp2或vp3具有至少85%、至少90%、至少95%或至少97%的氨基酸同一性。
在另一个实施方案中,本发明提供进化支F的新AAV,包括(不限于)具有衍生自以下一种或多种的衣壳的AAV:hu.14/AAV9[SEQ ID NO:3和123]、hu.31[SEQID NO:1和121]与hu.32[SEQ ID NO:1和122]。
本发明的AAV进化支可用于各种目的,包括提供用于产生病毒载体与产生靶分子的现成的相关AAV库。本领域的技术人员明白这些进化支也可用作用于各种目的的工具。
II.新的AAV序列
本发明提供在本文中可互换称为克隆hu.14/28.4和huAAV9的具有新AAV血清型的核酸序列和氨基酸。这些序列用于构建高效转导肝、肌肉和肺的载体。该新AAV及其序列也用于各种其它目的。这些序列由Genbank提交并给予本文所鉴定的登录号。
本发明还提供许多新AAV的核酸序列和氨基酸序列。如下文所总结的,许多这些序列包括上述作为进化支的成员的序列。
来自进化支A的128.1/hu.43[SEQ ID NO:80和160,GenBank登录号AY530606]、修饰的hu.43[SEQ ID NO:236]、128.3/hu.44[SEQ ID NO:81和158,GenBank登录号AY530607]和130.4/hu.48[SEQ ID NO:78和157,GenBank登录号AY530611];
来自进化支B的52/hu.19[SEQ ID NO:62和133,GenBank登录号AY530584]、52.1/hu.20[SEQ ID NO:63和134,GenBank登录号AY530586]、54.5/hu.23[SEQ IDNO:60和137,GenBank登录号AY530589]、54.2/hu.22[SEQ ID NO:67和138,GenBank登录号AY530588]、54.7/hu.24[SEQ ID NO:66和136,GenBank登录号AY530590]、54.1/hu.21[SEQ ID NO:65和135,GenBank登录号AY530587]、54.4R/hu.27[SEQ ID NO:64和140,GenBank登录号AY530592]、46.2/hu.28[SEQ IDNO:68和130,GenBank登录号AY530593]、46.6/hu.29[SEQ ID NO:69和132,GenBank登录号AY530594]、修饰的hu.29[SEQ ID NO:225],172.1/hu.63[SEQ IDNO:171和195]和140.2/hu.52[SEQ ID NO:167和191];
来自进化支C的3.1/hu.9[SEQ ID NO:58和155,GenBank登录号AY530626]、16.8/hu.10[SEQ ID NO:56和156,GenBank登录号AY530576]、16.12/hu.11[SEQ IDNO:57和153,GenBank登录号AY530577]、145.1/hu.53[SEQ ID NO:176和186,GenBank登录号AY530615]、145.6/hu.55[SEQ ID NO:178和187,GenBank登录号AY530617]、145.5/hu.54[SEQ ID NO:177和188,GenBank登录号AY530616]、7.3/hu.7[SEQ ID NO:55和150,GenBank登录号AY530628]、修饰的hu.7[SEQ IDNO:226]、hu.18[SEQ ID NO:52和149,GenBank登录号AY530583]、33.4/hu.15[SEQID NO:50和147,GenBank登录号AY530580]、33.8/hu.16[SEQ ID NO:51和148,GenBank登录号AY530581]、58.2/hu.25[SEQ ID NO:49和146,GenBank登录号AY530591]、161.10/hu.60[SEQ ID NO:170和184,GenBank登录号AY530622]、H-5/hu.3[SEQ ID NO:44和145,GenBank登录号AY530595]、H-1/hu.1[SEQ IDNO:46和144,GenBank登录号AY530575]和161.6/hu.61[SEQ ID NO:174和185,GenBank登录号AY530623];
来自进化支D的2-15/rh.62[SEQ ID NO:33和114,GenBank登录号AY530573]、1-7/rh.48[SEQ ID NO:32和115,GenBank登录号AY530561]、4-9/rh.54[SEQ IDNO:40和116,GenBank登录号AY530567]、4-19/rh.55[SEQ ID NO:37和117,GenBank登录号AY530568]、修饰的cy.5[SEQ ID NO:227]、修饰的rh.13[SEQ IDNO:228]和修饰的rh.37[SEQ ID NO:229];
来自进化支E的30.10/pi.1[SEQ ID NO:28和93,GenBank登录号AY53055]、30.12/pi.2[SEQ ID NO:30和95,GenBank登录号AY 530554]、30.19/pi.3[SEQ IDNO:29和94,GenBank登录号AY530555]、LG-4/rh.38[SEQ ID NO:7和86,GenBank登录号AY 530558]、LG-10/rh.40[SEQ ID NO:14和92,GenBank登录号AY530559]、N721-8/rh.43[SEQ ID NO:43和163,GenBank登录号AY530560]、1-8/rh.49[SEQ IDNO:25和103,GenBank登录号AY530561]、2-4/rh.50[SEQ ID NO:23和108,GenBank登录号AY530563]、2-5/rh.51[SEQ ID NO:22和104,GenBank登录号530564]、3-9/rh.52[SEQ ID NO:18和96,GenBank登录号AY530565]、3-11/rh.53[SEQ IDNO:17和97,GenBank登录号AY530566]、5-3/rh.57[SEQ ID NO:26和105,GenBank登录号AY530569]、5-22/rh.58[SEQ ID NO:27和58,GenBank登录号530570]、修饰的rh.58[SEQ ID NO:232]、2-3/rh.61[SEQ ID NO:21和107,GenBank登录号AY530572]、4-8/rh.64[SEQ ID NO:15和99,GenBank登录号AY530574]、修饰的rh.64[SEQ ID NO:233]、3.1/hu.6[SEQ ID NO:5和84,GenBank登录号AY530621]、33.12/hu.17[SEQ ID NO:4和83,GenBank登录号AY530582]、106.1/hu.37[SEQ IDNO:10和88,GenBank登录号AY530600]、LG-9/hu.39[SEQ ID NO:24和102,GenBank登录号AY530601]、114.3/hu.40[SEQ ID NO:11和87,GenBank登录号AY530603]、127.2/hu.41[SEQ ID NO:6和91,GenBank登录号AY530604]、127.5/hu.42[SEQ ID NO:8和85,GenBank登录号AY530605]、hu.66[SEQ ID NO:173和197,GenBank登录号AY530626]、hu.67[SEQ ID NO:174和198,GenBank登录号AY530627]和修饰的rh.2[SEQ ID NO:231];
来自进化支F的hu.14/AAV9[SEQ ID NO:3和123,GenBank登录号AY530579]、hu.31[SEQ ID NO:1和121,AY530596]和hu.32[SEQ ID NO:1和122,GenBank登录号AY530597]。
此外,本发明提供超出上述进化支范围的AAV序列,包括rh.59[SEQ ID NO:49和110]、rh.60[SEQ ID NO:31和120,GenBank登录号AY530571]、修饰的ch.5[SEQID NO:234]和修饰的rh.8[SEQ ID NO:235]。
也提供了本发明AAV序列的片段。每个这些片段可容易地用于各种载体系统和宿主细胞。理想的AAV片段中有cap蛋白,包括vp1、vp2、vp3和超变区。当需要时,描述于美国专利公开号US2003/0138772 A1(2003年7月24日)的方法可用于获得以上鉴定的AAV克隆的rep序列。这种rep序列包括,例如rep78、rep68、rep52和rep40与编码这些蛋白的序列。类似地,可使用描述于参考的专利出版物的技术来获得这些克隆的其它片段,包括AAV末端反向重复(ITR)、AAV P19序列、AAV P40序列、rep结合位点与末端分辨位点(terminal resolute site)(TRS)。其它合适的片段是本领域的技术人员容易明白的。
本发明的衣壳与其它片段可容易地用于各种载体系统和宿主细胞。这种片段可单独使用、与其它AAV序列或片段联用或与其它AAV或非AAV病毒序列的元件联用。在一个特别理想的实施方案中,载体含有本发明的AAV cap和/rep序列。
本发明的AAV序列与其片段可用于产生rAAV,也可用作反义递送载体、基因治疗载体或疫苗载体。本发明还提供含有本发明AAV序列的核酸分子、基因递送载体和宿主细胞。
可使用本文提供的信息来确定合适的片段。
如本文所述,含有本发明AAV衣壳蛋白的本发明载体尤其适用于其中中和抗体消除了基于其它AAV血清型的载体以及其它病毒载体的效力。本发明的rAAV载体特别有利于rAAV反复施用与重复基因治疗。
本发明的这些和其它实施方案及优点详述于下文。
A.AAV血清型9/hu14序列
本发明提供在本文中互换称为克隆hu.14(以前称为28.4)和huAAV9的新AAV的核酸序列与氨基酸。如本文所定义的,新血清型AAV9指具有以下衣壳的AAV,该衣壳产生与具有hu.14[SEQ ID NO:123]的序列的衣壳产生血清学交叉反应的抗体,并且该抗体不与针对AAV1、AAV2、AAV3、AAV4、AAV5、AAV6、AAV7或AAV8中任一种的衣壳所产生的抗体发生血清学交叉反应。
1.核酸序列
本发明的AAV9核酸序列包括由2211个核苷酸组成的SEQ ID NO:3的DNA序列。
本发明的核酸序列还包括与SEQ ID NO:3互补的链,以及对应于SEQ ID NO:3的RNA和cDNA序列及其互补链。本发明的核酸序列也包括SEQ ID NO:3的天然变体和工程改造的修饰物及其互补链。这种修饰物包括,例如本领域已知的标记、甲基化与用简并核苷酸取代一个或多个天然核苷酸。
本发明还包括与SEQ ID NO:3有约大于90%、优选至少约95%、最优选至少约98-99%相同或同源的核酸序列。
本发明也包括SEQ ID NO:3的片段、其互补链、以及与其互补的cDNA和RNA。合适的片段是至少15个核苷酸长,并且包括功能片段,即有生物学影响的片段。这种片段包括编码AAV9/HU.14衣壳的3种可变蛋白(vp)的序列,这3种可变蛋白是可变剪接变体:vp1[SEQ ID NO:3的nt1-2211]、vp2[SEQ ID NO:3约nt411-2211]与vp3[SEQ ID NO:3约nt609-2211]。SEQ ID NO:3的其它合适片段包括含有AAV9/HU.14衣壳蛋白的起始密码子的片段与本文所述编码vp1衣壳蛋白的超变区的片段。
除了包括附图与序列表所提供的核酸序列,本发明还包括设计为表达本发明的AAV血清型的氨基酸序列、蛋白质和肽的核酸分子和序列。因此,本发明包括使用这些序列和/或其独特的片段来编码以下产生的新AAV氨基酸序列和人工AAV血清型的核酸序列。
本文使用的人工AAV血清型包括(不限于)具有非天然衣壳蛋白的AAV。这种人工衣壳可通过任何合适的技术联用本发明的新AAV序列(例如,vp1衣壳蛋白的片段)与异源序列来产生,所述异源序列得自另一种AAV血清型(已知或是新的)、同一AAV血清型的非连续部分、非AAV病毒源或非病毒源。人工AAV血清型可是(不限于)嵌合AAV衣壳、重组AAV衣壳或“人源化”AAV衣壳。
2.HU.14/AAV9氨基酸序列、蛋白质和肽
本发明还提供由本发明的hu.14/AAV9核酸编码的蛋白质和其片段,与通过其它方法产生的hu.14/AAV9蛋白质和片段。本文使用的这些蛋白包括装配的衣壳。本发明还包括使用本发明的新AAV血清型的序列产生的AAV血清型,这些血清型是使用本领域技术人员已知的合成、重组或其它技术产生的。本发明不限于本发明的新AAV核酸序列表达的新AAV氨基酸序列、肽和蛋白质,而是包括通过本领域已知的其它方法,例如通过化学合成、其它合成技术或其它方法产生的氨基酸序列、肽和蛋白质。本文提供的任一AAV衣壳的序列可使用各种技术容易地产生。
合适的生产技术是本领域技术人员熟知的。参见,例如Sambrook等,《分子克隆:实验室手册》(Molecular Cloning:A Laboratory Manual),冷泉港出版社(冷泉港,纽约)。或者也可通过熟知的固相肽合成方法(Merrifield,J.Am.Chem.Soc.,85:2149(1962);Stewart和Young,《固相肽合成》(Solid Phase PeptideSynthesis)(Freeman,旧金山,1969)27-62页)来合成肽。这些或其它合适的生产方法是本领域技术人员已知的并且不限制本发明。
特别理想的蛋白质包括由以上鉴定的核苷酸序列所编码的AAV衣壳蛋白。该AAV衣壳蛋白包括3种可变剪接变体的蛋白:vp1、vp2和vp3。图2提供了vp1的全长序列。AAV9/HU.14衣壳蛋白包括vp1[SEQ ID NO:123的氨基酸(aa)1-736]、vp2[SEQ ID NO:123的约aa138-736]、vp3[SEQ ID NO:123的约aa203-736]及其功能片段。该衣壳蛋白的其它理想的片段包括位于超变区(HVR)之间的恒定区和可变区。该衣壳蛋白的其它理想的片段包括HVR本身。
开发出以确定AAV2中序列趋异的区域的算法得到了12个超变区(HVR),其中的5个与前文所述的4个可变区重叠或是其一部分。[Chiorini等,J.Virol,73:1309-19(1999);Rutledge等,J.Virol.,72:309-319]使用该算法和/或本文所述的对比技术确定了新AAV血清型的HVR。例如,HVR位于以下位置:HVR1,aa146-152;HVR2,aa182-186;HVR3,aa262-264;HVR4,aa381-383;HVR5,aa450-474;HVR6,aa490-495;HVR7,aa500-504;HVR8,aa514-522;HVR9,aa534-555;HVR10,aa581-594;HVR11,aa658-667;与HVRI2,aa705-719[该编号系统是基于使用AAV2 vp1作为参考点的对比方法]。使用默认设置的Clustal X程序,或使用其它市售或公用的默认设置的对比程序,例如本文所述的来进行本文所提供的对比方法,本领域技术人员可容易地确定本发明的新AAV衣壳的对应片段。
AAV9/HU.14衣壳蛋白的其它理想的片段包括SEQ ID NO:123的氨基酸1-184、氨基酸199-259、氨基酸274-446、氨基酸603-659、氨基酸670-706、SEQID NO:123的氨基酸724-736、aa185-198、aa260-273、aa447-477、aa495-602、aa660-669与aa707-723。此外,AAV衣壳的其它合适的片段包括:按照AAV9[SEQID NO:123]的编号为aa24-42、aa25-28、aa81-85、aa133-165、aa134-165、aa137-143、aa154-156、aa194-208、aa261-274、aa262-274、aa171-173、aa413-417、aa449-478、aa494-525、aa534-571、aa581-601、aa660-671与aa709-723。使用默认设置的ClustalX程序,或使用其它市售或公用的默认设置的对比程序来进行本文所提供的对比方法,本领域技术人员可容易地确定本发明新AAV衣壳的对应片段。
其它理想的AAV9/HU.14蛋白包括rep蛋白,例如rep68/78和rep40/52。
合适的片段至少长8个氨基酸。然而,可容易地使用其它所需长度的片段。这种片段可重组生产或通过其它合适的方法生产,例如化学合成。
本发明还提供使用本文提供的序列信息鉴定的其它AAV9/HU.14序列。例如,就本文提供的AAV9/HU.14序列而言,可使用基因组步行技术(genome walkingtechnology)(Siebert等,1995,Nucleic Acid Research,23:1087-1088;Friezner-Degen等,1986,J.Biol.Chem.261:6972-6985,BD Biosciences Clontech,Palo Alto,CA)分离感染性AAV9/HU.14。基因组步行技术特别适用于鉴定和分离与按照本发明的方法鉴定的新序列毗连的序列。基于本文提供的新AAV衣壳与rep序列,该技术也用于分离新AAV9/HU.14血清型的末端反向重复(ITR)。
本发明的序列、蛋白质和片段可通过任何合适的方法生产,包括重组生产、化学合成或其它合成方法。这种生产方法是本领域技术人员已知的并且不限制本发明。
III.具有新AAV衣壳的rAAV的产生
本发明包括不含与这些病毒天然相关的DNA和/或细胞物质的新AAV衣壳序列。为避免重复本文提供的所有新的AAV衣壳,在本章节与以下章节中参考hu.14/AAV9衣壳。然而,应该理解的是,本发明的其它新的AAV衣壳序列可以类似的方式使用。
本发明另一方面提供利用本发明的新AAV序列(包括其片段)来产生用于将异源基因或其它核酸序列递送至靶细胞的分子。
本发明另一方面提供利用本发明的新AAV序列(包括其片段)来产生用于将异源基因或其它核酸序列递送至靶细胞的病毒载体。
含有AAV序列的本发明分子包含有可递送至宿主细胞的任何遗传元件(载体),例如传送携带于其上的序列的裸DNA、质粒、噬菌体、转座子、粘粒、附加体、非病毒递送载体中的蛋白质(如,基于脂质的运载体)、病毒等。所选择的载体可通过任何合适的方法递送,包括转染、电穿孔、脂质体递送、膜融合技术、高速DNA包被小球、病毒感染与原生质体融合。用于构建本发明的任何实施方案的方法是核酸操作领域的技术人员已知的并且包括遗传工程、重组工程与合成技术。参见,例如Sambrook等,《分子克隆:实验室手册》(Molecular Cloning:A LaboratoryManual),冷泉港出版社,冷泉港,纽约。
在一个实施方案中,本发明的载体尤其含有编码本发明的AAV衣壳或其片段的序列。在另一个实施方案中,本发明的载体至少含有编码AAV rep蛋白质或其片段的序列。本发明的载体可任选含有AAV cap和rep蛋白质。在同时含有AAV rep和cap的载体中,AAV rep和AAV cap序列起源于同一进化支的AAV。或者,本发明提供的载体中rep序列来自不同于提供cap序列的AAV源。在一个实施方案中,rep和cap序列从单独的来源(例如,单独的载体,或宿主细胞和载体)表达。在另一个实施方案中,这些rep序列与不同AAV源的cap序列框内融合以现成嵌合AAV载体。本发明的载体任选是包装在本发明AAV衣壳中的载体。这些载体与本文所述的其它载体还可含有包含所选择的转基因的小基因,该小基因两侧是AAV5′ITR和AAV3′ITR。
因此,在一个实施方案中,本文所述的载体含有编码来自单独的AAV序列(例如,AAV9/HU.14)的完整的AAV衣壳的核酸序列。这种衣壳可含有SEQ ID NO:123的氨基酸1-736。或者,这些载体含有编码人工衣壳的序列,这些衣壳含有与异源AAV或非AAV衣壳蛋白(或其片段)融合的AAV9/HU.14衣壳的一个或多个片段。这些人工衣壳蛋白选自AAV9/HU.14衣壳的非连续部分或来自其它AAV的衣壳。例如,rAAV可具有包含一个或多个AAV9/HU.14衣壳区域的衣壳蛋白,所述衣壳区域选自vp2和/或vp3,或选自以下的其片段:AAV9/HU.14衣壳蛋白,SEQ IDNO:123的氨基酸1-184、氨基酸199-259、氨基酸274-466、氨基酸603-659、氨基酸670-706、氨基酸724-738。在另一个实施方案中,可能需要将vp3蛋白质的起始密码子改变为GTG。或者,rAAV可含有一个或多个本文鉴定的AAV血清型9衣壳蛋白的超变区,或其它片段,包括(不限于)AAV9/HU.14衣壳的aa185-198、aa260-273、aa447-477、aa495-602、aa660-669与aa707-723。参见,SEQ ID NO:123。这些修饰可增加表达、产量和/或改进选择的表达系统中的纯化,或可用于另一个需要的目的(例如,改变向性或修改中和抗体表位)。
本文所述的载体,例如质粒可用于各种目的,但特别适用于产生含有衣壳的rAAV,所述衣壳包含AAV序列或其片段。这些载体,包括rAAV、它们的元件、构建物和应用详述于本文。
本发明一方面提供一种产生具有AAV血清型9衣壳或其一部分的重组腺伴随病毒(AAV)的方法。该方法涉及培养宿主细胞,该宿主细胞含有如本文所定义的编码AAV血清型9衣壳蛋白的核酸序列或其片段;功能性rep基因;最少含有AAV末端反向重复(ITR)和转基因的小基因;与足够的辅助功能因子以将小基因包装进AAV9/HU.14衣壳蛋白。
需要在宿主细胞中培养将AAV小基因包装进AAV衣壳的成分可以反式提供给宿主细胞。或者,可由使用本领域技术人员已知的方法改造为含有一种或多种所需成分的稳定宿主细胞提供所需成分(例如,小基因、rep序列、cap序列和/或辅助功能因子)。这种稳定的宿主细胞最适合含有在可诱导启动子控制下的所需成分。然而,所需成分可在结构启动子的控制下。在本文讨论适合与转基因一起使用的调节元件中提供了合适的可诱导和组成型启动子的例子。或者,选择的稳定宿主细胞可含有在组成型启动子控制下的选择成分与在一个或多个可诱导启动子控制下的其它选择成分。例如,产生的稳定的宿主细胞来源于293细胞(该细胞含有在组成型启动子控制下的E1辅助功能物),但含有在可诱导启动子控制下的rep和/或cap蛋白。还有其它的稳定宿主细胞可由本领域技术人员制备。
产生本发明的rAAV所需的小基因、rep序列、cap序列和辅助功能物可以任一遗传元件的形式递送至包装宿主细胞,所述遗传元件传送其上所携带的序列。选择的遗传元件可通过任何合适的方法,包括本文所述的方法来递送。用于构建本发明的任何实施方案的方法是核酸操作领域的技术人员已知的并且包括遗传工程、重组工程与合成技术。参见,例如Sambrook等,《分子克隆:实验室手册》(MolecularCloning:A Laboratory Manual),冷泉港出版社,冷泉港,纽约。类似地,产生AAV病毒颗粒的方法是熟知的并且合适方法的选择不限制本发明。参见,例如K.Fisher等,J.Virol.,70:520-532(1993)与美国专利号5,478,745。
除非另有规定,本文所述的AAV ITR与其它选择的AAV成分可容易地选自任一AAV,包括(不限于)AAV1、AAV2、AAV3、AAV4、AAV5、AAV6、AAV7、AAV9与本发明的其它新AAV序列之一。这些ITR或其它AAV成分可使用本领域技术人员可用的技术容易地从AAV序列分离。这种AAV可分离自或得自学术、商业或公共来源(例如,美国模式培养物保藏所,Manassas,VA)。或者,AAV序列可参考,例如得自参考文献或数据库(例如,GenBank、PubMed等)中的公开序列通过合成或其它合适的方法获得。
A.小基因
小基因最少由转基因及其调节序列与5’和3’末端反向重复(ITR)组成。在一个理想的实施方案中,使用AAV血清型2的ITR。然而,也可选择其它合适来源的ITR。是将该小基因包装进衣壳蛋白并递送至选择的宿主细胞。
1.转基因
转基因是编码感兴趣的多肽、蛋白质或其它产物的核酸序列,它对其侧翼的载体序列是异源的。核酸编码序列以允许转基因在宿主细胞中转录、翻译和/或表达的方式操作性连接于调节成分。
转基因序列的组成取决于所得到的载体的用途。例如,某类型的转基因序列包括报道序列,该序列在表达后产生可检测的信号。这种报道序列包括(不限于)编码以下物质的DNA序列:β-内酰胺酶、β-半乳糖苷酶(LacZ)、碱性磷酸酶、胸腺激酶、绿色荧光蛋白(GFP)、增强型GFP(EGFP)、氯霉素乙酰转移酶(CAT)、荧光素酶、膜结合蛋白,如CD2、CD4、CD8,流感血凝素蛋白和其它本领域熟知的,这些物质存在高亲和力抗体或可通过常规方法产生所述抗体,以及含有膜结合蛋白的融合蛋白,该膜结合蛋白适当地与血凝素或Myc的抗原标记区域融合。
当这些编码序列与驱动其表达的调节元件相关联时,可提供由常规方法检测的信号,所述常规方法包括酶法、放射显影法、比色法、荧光法或其它光谱测定,荧光激活的细胞分选测定与免疫测定,包括酶联免疫吸附测定(ELISA)、放射性免疫测定(RIA)和免疫组织化学。例如,当标记序列是LacZ基因时,携带信号的载体的存在通过测定β-半乳糖苷酶活性来检测。当转基因是绿色荧光蛋白或荧光素酶时,携带信号的载体可用光度计通过颜色或光的产生来目测。
然而,转基因理想地是编码用于生物或医药的产物的非标记序列,所述产物例如蛋白质、肽、RNA、酶、显性负突变体或催化性RNA。理想的RNA分子包括tRNA、dsRNA、核糖体RNA、催化性RNA、siRNA、小发夹RNA、反式剪接RNA与反义RNA。有用的RNA序列的一个例子是抑制或消除靶核酸序列在处理的动物中表达的序列。合适的靶序列通常包括肿瘤靶点和病毒疾病。参见,就这种靶点的例子而言,肿瘤靶点与病毒在以下免疫原相关的章节中鉴定。
转基因可用于校正或改善基因缺陷,包括正常基因的表达低于正常水平的缺陷或功能基因产物不表达的缺陷。或者,转基因可为细胞提供天然不在该细胞类型中或该宿主中表达的产物。转基因序列的优选类型编码在宿主细胞中表达的治疗性蛋白质或多肽。本发明还包括使用多种转基因。在某些情况下,不同的转基因可用于编码一个蛋白质的各个亚基,或编码不同的肽或蛋白质。当编码蛋白质亚基的DNA的体积大,例如用于免疫球蛋白、血小板衍生的生长因子或抗肌萎缩蛋白是理想的。为使细胞产生多亚基蛋白,用含有每个不同亚基的重组病毒感染细胞。或者,蛋白质的不同亚基可由同一转基因编码。在该情况下,单个转基因包括编码每个亚基的DNA,而每个亚基的DNA由内部核糖体进入位点(IRES)隔开。当编码每个亚基的DNA的体积小,例如编码亚基的DNA与IRES的总体积小于5千碱基对时,这是理想的。除了IRES,DNA可用编码在翻译后阶段自我切割的2A肽的序列隔开。参见,例如,M.L.Donnelly等,J.Gen.Virol.,78(第一部分):13-21(1997年1月);Furler,S等,Gene Ther,8(11):864-873(2001年6月);Klump H等,Gene.Ther.,8(10):811-817(2001年5月)。该2A肽明显小于IRES,使得其非常适用于当间隔是限制因素时。更常见的是,当转基因是大的,含有多个亚基时,或当两个转基因共递送时,允许共施用携带所需转基因或亚基的rAAV以使得它们在体内多联体化以形成单一的载体基因组。在这种实施方案中,第一AAV可携带表达单一转基因的表达盒,而第二AAV可携带用于在宿主中共表达的不同转基因的表达盒。然而,选择的转基因可编码任何生物活性产物或其它产物,例如研究所需的产物。
本领域的技术人员可容易地选择合适的转基因。转基因的选择不限制本发明。
2.调节元件
除了以上鉴定的小基因的主要元件以外,该载体也包含有常规控制元件,该常规控制元件以允许转基因在细胞中转录、翻译和/或表达的方式操作性连接于转基因,所述细胞用本发明产生的质粒载体转染或病毒感染。本文使用的“操作性连接”的序列包括与感兴趣的基因毗连的表达控制序列与以反式起作用或在一定距离控制感兴趣基因的表达控制序列。
表达控制序列包括适当的转录起始子、终止子、启动子和增强子序列;有效的RNA加工信号,例如剪接与聚腺苷酸化(polyA)信号;稳定细胞质mRNA的序列;增强翻译效率的序列(即,Kozak共有序列);增强蛋白质稳定性的序列;以及当需要时增强编码的产物分泌的序列。许多表达控制序列,包括天然的、组成型、诱导型和/或组织特异性的启动子是本领域已知并可使用的。
组成型启动子的例子包括(不限于)逆转录病毒Rous肉瘤病毒(RSV)LTR启动子(任选具有RSV增强子)、巨细胞病毒(CMV)启动子(任选具有CMV增强子)[参见,例如Boshart等,Cell,41:521-530(1985)]、SV40启动子、二氢叶酸还原酶启动子、β-肌动蛋白启动子、磷酸甘油激酶(PGK)启动子与EF1启动子[Invitrogen]。诱导型启动子能调节基因表达并可由外源提供的化合物、环境因素(例如温度)、或存在特定的生理状态,例如急性期、细胞的特定分化状态所调节,或仅在复制型细胞中。可用各种商业来源的诱导型启动子和可诱导系统,包括(不限于)Invitrogen、Clontech和Ariad。许多其它系统已描述并可由本领域技术人员容易地选择。由外源提供的化合物调节的诱导型启动子的例子包括锌-诱导的金属硫蛋白(MT)启动子、地塞米松(Dex)-诱导的小鼠乳腺肿瘤病毒(MMTV)启动子、T7聚合酶启动子系统[国际专利公开号WO 98/10088]、蜕皮素昆虫启动子[No等,Proc.Natl.Acad.Sci.美国,93:3346-3351(1996)]、四环素可阻抑系统[Gossen等,Proc.Natl.Acad.Sci.美国,89:5547-5551(1992)]、四环素可诱导系统[Gossen等,Science,268:1766-1769(1996),也参见Harvey等,Curr.Opin.Chem.Biol.,2:512-518(1998)]、RU-486可诱导系统[Wang等,Nat.Biotech.,15:239-243(1997)与Wang等,Gene Ther.,4:432-441(1997)]与雷帕霉素可诱导系统[Magari等,J.Clin.Invest.,100:2856-2872(1997)]。其它可用于本发明的诱导型启动子是可由特定的生理状态,例如温度、急性期、细胞的特定分化状态所调节的启动子,或仅在复制型细胞中。
在另一个实施方案中使用了转基因的天然启动子。当需要转基因的表达模拟天然表达时,天然启动子较佳。当转基因的表达必须暂时性或发展性调节、或以组织特异性的方式调节或响应于特定的转录刺激时,可使用天然启动子。在其它实施方案中,也可使用其它天然表达控制元件,例如增强子元件、聚腺苷酸化位点或Kozak共有序列以模拟天然表达。
转基因的另一实施方案包含有操作性连接于组织特异性启动子的基因。例如,如果需要在骨骼肌中表达,应该使用在肌肉中有活性的启动子。这些包括得自编码以下产物的基因的启动子:骨骼β-肌动蛋白、肌球蛋白轻链2A、抗肌萎缩蛋白、肌肉肌酸激酶以及活性高于天然启动子的合成肌肉启动子(参见Li等,Nat.Biotech.,17:241-245(1999))。组织特异性启动子的例子已知有肝(白蛋白,Miyatake等,J.Virol.,71:5124-32(1997);乙肝病毒核心启动子,Sandig等,Gene Ther.,3:1002-9(1996);α-胎蛋白(AFP),Arbuthnot等,Hum.Gene Ther.,7:1503-14(1996))、骨的骨钙蛋白(Stein等,Mol Biol.Rep.,24:185-96(1997))、骨唾液蛋白(Chen等,J.BoneMiner.Res.,11:654-64(1996))、淋巴细胞(CD2,Hansal等,J.Immunol.,161:1063-8(1998);免疫球蛋白重链;T细胞受体链)、神经元,例如神经元-特异性烯醇酶(NSE)启动子(Andersen等,Cell.Mol.Neurobiol.,13:503-15(1993))、神经丝轻链基因(Piccioli等,Proc.Natl.Acad.Sci.,美国,88:5611-5(1991))与神经元-特异性vgf基因(Piccioli等,Neuron,15:373-84(1995))等。
携带治疗有用的转基因的质粒也可任选含有可选择标记,或者报道基因可含有编码遗传霉素、潮霉素或嘌呤霉素(purimycin)抗性等的序列。这种可选择报道基因或标记基因(优选位于病毒基因组外从而由本发明的方法所拯救)可用来预示细菌细胞中存在质粒,例如氨苄青霉素抗性。质粒的其它成分包括复制起点。可通过常规方法选择这些和其它启动子与载体元件并且许多这种序列可用[参见,例如Sambrook等,与本文引用的参考文献]。
为便于本文参考,转基因、启动子/增强子与5′和3′AAV ITR的组合称为“小基因”。依靠本文提供的指导,可凭借常规技术设计这种小基因。
3.将小基因递送至包装宿主细胞
任何递送至宿主细胞的合适载体,例如质粒上可携带小基因。用于本发明的质粒可改造为适合于在原核细胞、哺乳动物细胞(或二者)中复制以及任选整合。这些质粒(或其它携带5′AAV ITR-异源分子-3′AAV ITR的载体)含有允许小基因在真核细胞和/或原核细胞中复制的序列与用于这些系统的选择标记。可选择标记或报道基因可含有编码遗传霉素、潮霉素或嘌呤霉素抗性等的序列。这些质粒也可含有可用来预示细菌细胞中存在载体(例如氨苄青霉素抗性)的某些可选择报道基因或标记基因。质粒的其它成分可含有复制起点和扩增子,例如使用Epstein Barr病毒核抗原的扩增子系统。该扩增子系统或其它类似的扩增子成分允许细胞中高拷贝附加型复制。携带小基因的分子优选转染进其可暂时存在的细胞。或者,小基因(携带5′AAV ITR-异源分子-3′AAV ITR)可稳定地整合进宿主细胞基因组、或在染色体上或作为附加体。在某些实施方案中,小基因可以多份拷贝存在,任选采用头-对-头、头-对-尾或尾-对-尾多联体形式。合适的转染技术是已知的并可容易地用于将小基因递送至宿主细胞。
当通过转染递送含有小基因的载体时,递送到约1×104个细胞-1×1013个细胞,或约1×105个细胞的该载体的量一般约为5μg-100μg DNA、约10μg-50μgDNA。然而,考虑到诸如所选择的载体、递送方法和所选择的宿主细胞等因素,可调整递送至宿主细胞的载体DNA的相对量。
B.Rep与Cap序列
除了小基因以外,宿主细胞含有驱动本发明的新AAV衣壳蛋白(或含有其片段的衣壳蛋白)在宿主细胞中表达的序列与和小基因中发现的AAV ITR具有相同来源或交叉互补来源的rep序列。AAV cap和rep序列可独立得自上述AAV源并可以上述本领域人员已知的任何方式引入宿主细胞。此外,当AAV载体为假型时(例如,AAV9/HU.14衣壳),可用不同的AAV源(例如,AAV1、AAV2、AAV3、AAV4、AAV5、AAV6、AAV7、AAV8)提供编码每种主要rep蛋白的序列。例如,rep78/68序列得自AAV2,而rep52/40序列得自AAV8。
在一个实施方案中,宿主细胞稳定地含有在合适的启动子(例如上述的)控制下的衣壳蛋白。在该实施方案中,衣壳蛋白最好在诱导型启动子的控制下表达。在另一个实施方案中,衣壳蛋白以反式提供至宿主细胞。当衣壳蛋白以反式递送至宿主细胞时,它可通过含有引导选择的衣壳蛋白在宿主细胞中表达的所需序列的质粒递送。当质粒以反式递送至宿主细胞时,携带衣壳蛋白的质粒也最好携带包装AAV所需的其它序列,例如rep序列。
在另一个实施方案中,宿主细胞稳定地含有在合适的启动子(例如上述的)控制下的rep序列。在该实施方案中,主要的rep蛋白最好在诱导型启动子的控制下表达。在另一个实施方案中,rep蛋白以反式提供至宿主细胞。当rep蛋白以反式递送至宿主细胞时,它可通过含有引导选择的rep蛋白在宿主细胞中表达的所需序列的质粒递送。当质粒以反式递送至宿主细胞时,携带衣壳蛋白的质粒也最好携带包装AAV所需的其它序列,例如rep和cap序列。
因此,在一个实施方案中,rep和cap序列可在单独的核酸分子上转染进宿主细胞并且作为附加体稳定地存在于细胞中。在另一个实施方案中,rep和cap序列稳定地整合进细胞的染色体。在另一个实施方案中,rep和cap序列在宿主细胞中瞬时表达。例如,用于这种转染的有用核酸分子从5’到3’含有启动子、插在启动子与rep基因序列起始点之间任选的间隔子、AAV rep基因序列与AAV cap基因序列。
rep和/或cap序列可任选提供于载体上,该载体含有待引入宿主细胞的DNA序列。例如,载体可含有包含小基因的rAAV构建物。载体可含有一个或多个编码辅助功能物,例如腺病毒蛋白E1、E2a和E4 ORF6的基因与用于VAI RNA的基因。
用于该构建物的启动子优选本领域技术人员已知的或上述任何组成型、诱导型或天然启动子。一个实施方案使用AAV P5启动子序列。选择AAV来提供任何这些序列不是对本发明的限制。
在另一个优选的实施方案中,rep的启动子是诱导型启动子,例如上述与转基因调节元件相连的。Rep表达优选的启动子是T7启动子。包含由T7启动子调节的rep基因和cap基因的载体转染或转化进组成型性地或可诱导地表达T7聚合酶的细胞。参见1998年3月12日出版的国际专利公开号WO98/10088。
间隔子在设计载体中是可任选的元件。间隔子是插在启动子和rep基因ATG起始位点间的DNA序列。间隔子可具有任何所需的设计;即,它可是随机的核苷酸序列,或者可编码一个基因产物,例如标记基因。间隔子可含有通常包括起始/终止和polyA位点的基因。间隔子可是原核生物或真核生物的非编码DNA序列、重复非编码序列、无转录控制的编码序列或有转录控制的编码序列。间隔子序列的两个示范性来源是噬菌体梯序列或酵母梯序列,二者购自,例如Gibco或Invitrogen等。间隔子可是任何大小只要足以降低rep78和rep68基因产物的表达,使rep52、rep40和cap基因产物的表达量处于正常水平。因此,间隔子的长度可约为10bp-10.0kbp、优选约100bp-8.0kbp。为减少重组的可能性,间隔子长度优选小于2kbp;然而,本发明不限于此。
虽然提供rep和cap的分子可瞬时存在于宿主细胞中(即,通过转染),但rep和cap蛋白之一(或二者)与控制其表达的启动子宜在宿主细胞中稳定地表达,例如作为附加体或通过整合进宿主细胞的染色体。用于构成本发明的实施方案的方法是常规遗传工程或重组工程技术,例如以上参考文献所述的。虽然本说明书提供了特定的构建物的说明性例子,但本领域的技术人员可使用本文提供的信息来选择间隔子、P5启动子和其它元件(包括至少一个翻译起始和终止信号)与任选加入的聚腺苷酸化位点,从而可选择和设计其它合适的构建物。
在本发明的另一个实施方案中,可由宿主细胞稳定地提供rep或cap蛋白。
C.辅助功能物
为包装本发明的rAAV,宿主细胞也需要辅助功能物。这些功能物可任选由疱疹病毒提供。所需的辅助功能物最好分别由人或非人灵长类腺病毒源提供,例如上述的和/或得自各种来源,包括美国模式培养物保藏所(ATCC),Manassas,VA(美国)。在一个现在优选的实施方案中,宿主细胞与以下基因产物一起提供和/或含有以下基因产物:E1a基因产物、E1b基因产物、E2a基因产物和/或E4 ORF6基因产物。宿主细胞可含有其它腺病毒基因,例如VAI RNA,但这些基因不是必需的。在优选的实施方案中,宿主细胞中不存在其它腺病毒基因或基因功能物。
“表达E1a基因产物的腺病毒DNA”表示编码E1a或任何功能性E1a部分的任何腺病毒序列。表达E2a基因产物的腺病毒DNA与表达E4 ORF6基因产物的腺病毒DNA的定义类似。也包括任何等位基因或腺病毒基因的其它修饰物或其功能部分。可借助常规遗传工程或诱变技术,以及其天然的等位变体来有意引入这种修饰,从而以某种方式增强腺病毒功能。本领域的技术人员已知这种修饰物和用于操作DNA以获得这些腺病毒基因功能物的方法。
腺病毒E1a、E1b、E2a和/或E4 RF6基因产物以及任何其它所需的辅助功能物可使用任何使其在细胞中表达的方法提供。每条编码这些产物的序列可位于独立的载体上,或者一个或多个基因可位于同一载体上。载体可是本领域已知的或以上披露的任何载体,包括质粒、粘粒和病毒。载体可通过本领域已知或以上披露的任何方法引入宿主细胞,所述方法包括转染、感染、电穿孔、脂质体递送、膜融合技术、高速DNA-包被小球、病毒感染与原生质体融合等。一个或多个腺病毒基因可稳定地整合进宿主细胞基因组、稳定地表达为附加体或瞬时表达。基因产物可全部瞬时表达、表达在附加体上或稳定地整合,或者一些基因产物可稳定地表达,而另一些瞬时表达。此外,每个腺病毒基因的启动子可独立选自组成型启动子、诱导型启动子或天然腺病毒启动子。例如,启动子可由生物体或细胞的特定生理状态调节(即,由分化状态或在复制或静息细胞中)或由外源性添加的因素调节。
D.宿主细胞和包装细胞系
宿主细胞本身可选自任何生物体,包括原核(例如,细菌)细胞和真核细胞,包括昆虫细胞、酵母细胞和哺乳动物细胞。特别理想的宿主细胞选自任何哺乳动物种类,包括(不限于)以下细胞:A549、WEH1、3T3、10T1/2、BHK、MDCK、COS1、COS7、BSC1、BSC40、BMT10、VERO、W138、HeLa、293细胞(表达功能性腺病毒E1)、Saos、C2C12、L细胞、HT1080、HepG2与来源于哺乳动物(包括人、猴、小鼠、大鼠、家兔和仓鼠)的初级成纤维细胞、肝细胞和成肌细胞。提供细胞的哺乳动物种类与哺乳动物细胞类型(即,成纤维细胞、肝细胞、肿瘤细胞等)的选择不限制本发明。所用细胞的要求是:它不携带任何除E1、E2a和/或E4 ORF6以外的腺病毒基因;它不含有在rAAV产生过程中可导致污染病毒的同源重组的任何其它病毒基因;并且它能感染或转染DNA并表达所转染的DNA。在优选的实施方案中,宿主细胞是rep和cap在细胞中稳定地转染的细胞。
用于本发明的宿主细胞是用编码rep和cap的序列稳定转化并用腺病毒E1、E2a和E4 ORF6 DNA和携带有上述小基因的构建物转染的宿主细胞。也可类似地使用稳定的rep和/或cap表达细胞系,例如B-50(国际专利申请公开号WO99/15685),或美国专利号5,658,785所述的细胞系。另一种较好的宿主细胞含有足以表达E4 ORF6的最少腺病毒DNA。还有其它细胞系可使用本发明的新AAV9 cap序列构建。
制备本发明的宿主细胞涉及诸如装配选择的DNA序列的技术。可使用常规技术实现这种装配。这种技术包括熟知的且描述于上文引用的Sambrook等的cDNA和基因组克隆、使用腺病毒和AAV基因组的重叠寡核苷酸序列、与聚合酶链式反应结合、合成方法与其它提供所需核苷酸序列的合适方法。
也可使用技术人员已知和说明书中讨论的技术将这些分子(质粒或病毒)引入宿主细胞。在优选的实施方案中,使用标准转染技术,例如CaPO4转染或电穿孔和/或通过感染将杂交腺病毒/AAV载体引入细胞系,例如人胚胎肾细胞系HEK293(提供反式激活E1蛋白质的含功能性腺病毒E1基因的人肾细胞系)。
因为在人群中未发现针对AAV9/HU.14的中和抗体,本领域技术人员所制备的基于AAV9/HU.14的载体有利于将基因递送至选择的宿主细胞和基因治疗的患者。本领域的技术人员可使用已知的各种技术来容易地制备其它含有本文提供的AAV9/HU.14衣壳蛋白的rAAV病毒载体。类似地,人们还可制备其它含有AAV9/HU.14序列和其它来源的AAV衣壳蛋白的rAAV病毒载体。
本领域技术人员易于理解本发明的新AAV序列可容易地适用于体外、离体或体内基因递送的这些和其它病毒载体系统。类似地。本领域技术人员可容易地选择用于各种rAAV和非rAAV载体系统的本发明AAV基因组的其它片段。这种载体系统可包括如慢病毒、逆转录病毒、痘病毒、牛痘病毒和腺病毒系统等。这些载体系统的选择不限制本发明。
因此,本发明还提供使用本发明的新AAV的核酸与氨基酸序列产生的载体。这种载体用于各种目的,包括递送治疗性分子与用在疫苗给药方案中。含有本发明的新AAV的衣壳的重组AAV对递送治疗性分子特别理想。含有本发明的新AAV序列的这些或其它载体构建物可用于疫苗给药方案,例如用于共同递送细胞因子或用于递送免疫原本身。
IV.重组病毒及其应用
本领域的技术人员可使用本文所述的技术来产生rAAV,该rAAV具有本发明AAV的衣壳或其衣壳含有本发明AAV的一个或多个片段。一个实施方案使用单一AAV的全长衣壳,例如hu.14/AAV9[SEQ ID NO:123]。另一个实施方案中产生的全长衣壳含有本发明新AAV衣壳的一个或多个片段与另一个选择的AAV的序列或同一AAV的异源(即,非连续)部分融合。例如,rAAV可含有AAV9/HU.14的一个或多个新的超变区序列。或者,本发明的独特AAV序列可用于含有其它病毒或非病毒序列的构建物。重组病毒可任选携带编码一种或多种AAV rep蛋白的AAV rep序列。
A.递送病毒
本发明另一方面提供一种将转基因递送至宿主的方法,该方法涉及用本发明AAV9/HU.14序列(或其功能性片段)产生的重组病毒载体转染或感染所选宿主细胞。用于递送的方法是本领域技术人员熟知的并不限制本发明。
在一个较好的实施方案中,本发明提供用于AAV介导递送转基因至宿主的方法。该方法涉及用含有所选转基因和AAV9衣壳蛋白的重组病毒载体转染或感染所选宿主细胞,该转基因在指导其表达的序列的控制下。
宿主的样品可任选首先测定针对所选AAV源(例如,一种血清型)的抗体的存在。用于检测中和抗体的各种测定形式为本领域技术人员所熟知。选择这种测定不限制本发明。参见,例如Fisher等,Nature Med.,3(3):306-312(1997年3月)和W.C.Manning等,Human Gene Therapy,9:477-485(1998年3月1日)。该测定的结果可用于确定哪种含有特定来源的衣壳蛋白的AAV载体是递送所优选的,例如通过缺失针对该衣壳来源特异的中和抗体。
在该方法的一个方面,可在通过具有不同AAV衣壳蛋白的载体递送基因之前或之后递送具有本发明AAV衣壳蛋白的载体。因此,通过rAAV载体的基因递送可用于将基因反复递送至所选的宿主细胞。随后给予的rAAV载体宜携带相同于第一rAAV载体的转基因,但随后给予的载体含有衣壳蛋白来源不同于第一载体(优选不同的血清型)。例如,如果第一载体具有AV9/HU.14衣壳蛋白,随后给予的载体可具有选自其它AAV的衣壳蛋白,任选来自另一血清型或另一进化支。
多个rAAV载体可任选通过共同给予rAAV载体来递送大的转基因或多个转基因,所述rAAV载体在体内多联体化以形成单一载体基因组。在这种实施方案中,第一AAV可携带表达单一转基因(或其亚基)的表达盒,第二AAV可携带表达第二转基因(或不同亚基)的表达盒从而在宿主细胞中共同表达。第一AAV可携带多顺反子构建物的第一部分(例如,启动子和转基因,或亚基)的表达盒,第二AAV可携带多顺反子构建物的第二部分(例如,转基因或亚基和polyA序列)的表达盒。多顺反子构建物的这两个部分在体内多联体化以形成共同表达由第一和第二AAV递送的转基因的一个载体基因组。在这种实施方案中,携带第一表达盒的rAAV载体和携带第二表达盒的rAAV载体可在单个药物组合物中递送。在其它实施方案中,两个或多个rAAV载体以分开的药物组合物递送,这些药物组合物可基本上同时或一个紧接着另一个给予。
上述重组载体可按照公开的方法递送至宿主细胞。优选悬浮在生理相容运载体中的rAAV可给予人或非人哺乳动物患者。本领域的技术人员可依据传送病毒的指示容易地选择合适的运载体。例如,一种合适的运载体包括可用各种缓冲溶液配制的盐水(例如,磷酸缓冲盐水)。其它示范性运载体包括无菌盐水、乳糖、蔗糖、磷酸钙、明胶、葡萄糖、琼脂、果胶、花生油、芝麻油和水。运载体的选择不限制本发明。
本发明组合物可任选含有除rAAV和运载体以外的其它常规药物组分,例如防腐剂或化学稳定剂。合适的示范性防腐剂包括氯代丁醇、山梨酸钾、山梨酸、二氧化硫、没食子酸丙酯、对羟基苯甲酸酯类、乙基香草醛、甘油、苯酚与对氯苯酚。合适的化学稳定剂包括明胶和白蛋白。
载体的给予量足以转染细胞并提供足够的基因传送和表达水平以提供治疗性益处而无过度不良作用或具有医学上可接受的生理作用,这可由医学领域技术人员确定。常规的和药学上可接受的给药途径包括(但不限于)直接递送至所需器官(例如,肝(任选经肝动脉)或肺)、口服、吸入、鼻内、气官内、动脉内、眼内、静脉内、肌肉内、皮下、真皮内和其它胃肠外给药途径。如果需要,可联合给药途径。
病毒载体的剂量主要取决于以下因素,例如待治疗的病症、患者的年龄、体重和健康状况,因此可依患者而变。例如,病毒载体的治疗有效的人用剂量一般约为0.1mL-100mL含有浓度约1×109-1×1016个基因组病毒载体的溶液。递送至大器官(例如,肝、肌肉、心脏和肺)的优选人用剂量可约为5×1010-5×1013个AAV基因组/kg,体积约为1-100mL。递送至眼的优选剂量约为5×109-5×1012份基因组拷贝,体积约为0.1mL-1mL。可调整剂量以平衡抗任何副作用的治疗性益处并且这种剂量可依所采用的重组载体治疗应用而变。可监测转基因的表达水平来确定导致病毒载体、优选含有小基因的AAV载体的剂量频率。可任选采用类似于治疗性目的所述的给药方案用本发明组合物来免疫接种。
由本发明的含AAV的载体递送的治疗性产物和免疫原性产物的例子于下文提供。这些载体可用于本文所述的各种治疗性或接种方案。此外,在所需的治疗性和/或接种方案中,这些载体可联合一种或多种其它载体或活性成分递送。
B.治疗性转基因
转基因编码的有用的治疗性产物包括激素与生长和分化因子,包括(不限于)胰岛素、胰高血糖素、生长激素(GH)、甲状旁腺激素(PTH)、生长激素释放因子(GRF)、卵泡刺激素(FSH)、黄体激素(LH)、人绒毛膜促性腺激素(hCG)、血管内皮生长因子(VEGF)、促血管生成素、血管抑制素、粒细胞集落刺激因子(GCSF)、促红细胞生产素(EPO)、结缔组织生长因子(CTGF)、碱性成纤维细胞生长因子(bFGF)、酸性成纤维细胞生长因子(aFGF)、表皮生长因子(EGF)、血小板衍生的生长因子(PDGF)、胰岛素生长因子I和II(IGF-1和IGF-II)、转化生长因子α超家族中的任一个(包括TGFα)、活化素、抑制素、或骨形态发生蛋白(BMP)中的任一个BMP1-15、生长因子的调蛋白/神经调节蛋白/ARIA/neu分化因子(NDF)家族的任一个、神经生长因子(NGF)、脑衍生神经营养因子(BDNF)、神经营养蛋白NT-3和NT-4/5、睫状神经营养因子(CNTF)、胶质细胞系衍生神经营养因子(GDNF)、neurturin、聚集蛋白、脑信号蛋白/瓦解蛋白家族的任一个、导蛋白-1和导蛋白-2、肝细胞生长因子(HGF)、肝配蛋白、头蛋白、sonic hedgehog蛋白和酪氨酸羟化酶。
其它有用的基因产物包括调节免疫系统的蛋白,包括(不限于)细胞因子和淋巴因子,例如血小板生成素(TPO)、白介素(IL)IL-1到IL-25(如IL-2、IL-4、IL-12和IL-18)、单核细胞化学诱导物蛋白、白血病抑制因子、粒细胞-巨噬细胞集落刺激因子、Fas配体、肿瘤坏死因子α和β、干扰素α、β和γ、干细胞因子、flk-2/flt3配体。免疫系统产生的基因产物也可用于本发明。这些产物包括(不限于)免疫球蛋白IgG、IgM、IgA、IgD和IgE、嵌合免疫球蛋白、人源化抗体、单链抗体、T细胞受体、嵌合T细胞受体、单链T细胞受体、I类和II类MHC分子以及改造的免疫球蛋白和MHC分子。有用的基因产物也包括补体调节蛋白,例如补体调节蛋白、膜辅因子蛋白(MCP)、衰变加速因子(DAF)、CR1、CF2和CD59。
其它有用的基因产物包括激素受体、生长因子、细胞因子、淋巴因子、调节蛋白和免疫系统蛋白的任一种。本发明包括用于胆固醇调节和/或脂质调节的受体,包括低密度脂蛋白(LDL)受体、高密度脂蛋白(HDL)受体、极低密度脂蛋白(VLDL)受体和清除受体。本发明也包括以下基因产物:例如类固醇激素受体超家族的成员,包括糖皮质激素受体和雌激素受体。维生素D受体和其它核受体。此外,有用的基因产物包括转录因子,例如jun、fos、max、mad、血清效应因子(SRF)、AP-1、AP2、myb、MyoD和肌细胞生成蛋白、含有蛋白质的ETS-盒、TFE3、E2F、ATF1、ATF2、ATF3、ATF4、ZF5、NFAT、CREB、HNF-4、C/EBP、SP1、CCAAT-盒结合蛋白、干扰素调节因子(IRF-1)、Wilms肿瘤蛋白、ETS-结合蛋白、STAT、GATA-盒结合蛋白,例如GATA-3和翼状螺旋蛋白的叉头蛋白家族。
其它有用的基因产物包括氨甲酰合成酶1、鸟氨酸转氨甲酰酶、精氨基琥珀酸合成酶、精氨基琥珀酸裂合酶、精氨酸酶、延胡索酰乙酰乙酸水解酶、苯丙氨酸羟化酶、α-1抗胰蛋白酶、葡萄糖-6-磷酸酶、胆色素原脱氨酶、胱硫醚β合成酶、支链酮酸脱羧酶、白蛋白、异戊酰-CoA脱氢酶、丙酰-CoA羧化酶、甲基丙二酰CoA变位酶、戊二酰-CoA脱氢酶、胰岛素、β-葡糖苷酶、丙酮酸羧酸盐、肝磷酸化酶、磷酸化酶激酶、甘氨酸脱羧酶、H-蛋白、T-蛋白、囊性纤维化跨膜传导调节蛋白(CFTR)序列和抗肌萎缩蛋白基因产物[例如,小-或微-抗肌萎缩蛋白]。其它有用的基因产物包括可用于酶活性缺乏所导致的各种病症,例如可用于酶替代治疗的酶。例如,含有甘露糖-6-磷酸的酶可用于治疗溶菌酶储存疾病(例如,含有编码β-葡糖醛酸糖苷酶(GUSB)的合适基因)。
其它有用的基因产物包括用于治疗血友病的产物,包括B型血友病(包括因子IX)和A型血友病(包括因子VIII及其变体,例如杂二聚体和B-缺失结构域的轻链和重链;美国专利号6,200,560和6,221,349)。因子VIII基因编码2351个氨基酸,该蛋白具有6个结构域,从氨基向羧基端依次指定为A1-A2-B-A3-C1-C2[Wood等,Nature,312:330(1984);Vehar等,Nature,312:337(1984)与Toole等,Nature,342:337(1984)]。人因子VIII在细胞内加工以产生主要含有重链和轻链的杂二聚体,所述重链含有A1、A2和B结构域,所述轻链含有A3、C1和C2结构域。单链多肽与杂二聚体作为无活性的前体在血浆中循环直至在A2和B结构域之间被凝血酶切割而激活,释放B结构域并导致由A1和A2结构域组成的重链。B结构域以该蛋白激活的前凝血质形式除去。此外,在天然蛋白质中,B结构域两侧的两条多肽链(“a”和“b”)与二价钙阳离子结合。
在一些实施方案中,小基因含有编码10个氨基酸信号序列以及人生长激素(hGH)聚腺苷酸化序列的因子VIII重链的前57个碱基对。在供选择的实施方案中,小基因还含有A1和A2结构域,以及B结构域N-末端的5个氨基酸和/或B结构域C末端的85个氨基酸以及A3、C1和C2结构域。在还有的实施方案中,编码因子VIII重链和轻链的核酸以被编码B结构域的14个氨基酸的42核酸隔开的单一小基因提供[美国专利号6,200,560]。
本文使用的治疗有效量指AAV载体的量可产生足够量的因子VIII以降低受试对象血液凝结所需时间。因子VIII水平低于正常水平的1%的严重血友病患者的血液凝结时间一般大于60分钟,而非血友病患者约为10分钟。
本发明不受任何具体的因子VIII序列的限制。已分离和产生了因子VIII的许多天然和重组形式。因子VIII的天然和重组形式的例子见专利和科学文献,包括美国专利号5,563,045;美国专利号5,451,521;美国专利号5,422,260;美国专利号5,004,803;美国专利号4,757,006;美国专利号5,661,008;美国专利号5,789,203;美国专利号5,681,746;美国专利号5,595,886;美国专利号5,045,455;美国专利号5,668,108;美国专利号5,633,150;美国专利号5,693,499;美国专利号5,587,310;美国专利号5,171,844;美国专利号5,149,637;美国专利号5,112,950;美国专利号4,886,876;国际专利公开号WO 94/11503;WO 87/07144;WO 92/16557;WO91/09122;WO 97/03195;WO 96/21035和WO 91/07490;欧洲专利申请号EP 0 672138;EP 0 270 618;EP 0 182 448;EP 0 162 067;EP 0 786 474;EP 0 533 862;EP0 506 757;EP 0 874 057;EP 0 795 021;EP 0 670 332;EP 0 500 734;EP 0 232 112和EP 0 160 457;Sanberg等,世界血友病联合会第20届国际会议(XXth Int.Congressof the World Fed Of Hemophilia),(1992)和Lind等,Eur.J.Biochem.,232:19(1995)。
编码上述因子VIII的核酸序列可使用重组方法或通过从已知包含该序列的载体衍生该序列得到。此外,可使用标准技术直接从含有该序列的细胞和组织直接分离所需序列,所述标准技术例如酚提取与cDNA或基因组DNA的PCR[参见,例如Sambrook等]。除了克隆以外,核苷酸序列也可合成产生。可用标准方法制备重叠的寡核苷酸,从重叠的寡核苷酸装配完整的序列并装配进完整的编码序列[参见,例如Edge,Nature,292:757(1981);Nambari等,Science,223:1299(1984)和Jay等,J.Biol.Chem.,259:6311(1984)]。
此外,本发明不限于人因子VIII。实际上,本发明应该包括除人以外的动物的因子VIII,所述动物包括(但不限于)陪伴动物(例如、犬、猫和马)、牲畜(例如,牛、山羊和绵羊)、实验室动物、海生动物、大型猫科动物(large cats)等。
AAV载体可含有编码因子VIII的片段的核酸,该片段本身无生物活性,但当施用进受试对象时改善或恢复血液凝结时间。例如,上述因子VIII蛋白含有两条多肽链:由B结构域隔开的重链和轻链,而B结构域在加工过程中被切断。如本发明所证明的,用因子VIII的重链和轻链共同转导受体细胞导致生物活性因子VIII的表达。由于大多数血友病患者仅在一条链(例如,重链或轻链)中有突变或缺失,可能仅施用患者有缺陷的链来补充另一条链。
其它有用的基因产物包括非天然多肽,例如具有天然氨基酸序列的嵌合或杂交多肽,而所述天然氨基酸序列含有插入、缺失或氨基酸取代。例如,单链改造的免疫球蛋白可用在某些免疫受损患者中。其它类型的非天然基因序列包括可用于降低靶过度表达的反义分子和催化性核酸,例如核酶。
降低和/或调节基因表达用于治疗以过度增殖细胞为特征的过度增殖疾病,例如癌症和银屑病尤其理想。靶多肽包括与正常细胞相比,专门地或在过度增殖细胞中较高水平产生的多肽。靶抗原包括癌基因与易位基因编码的多肽,所述癌基因例如是myb、myc、fyn,所述易位基因如bcr/abl、ras、src、P53、neu、trk和EGRF。除了作为靶抗原的癌基因产物,用于抗癌治疗和保护性治疗方案的靶多肽包括B细胞淋巴瘤产生的抗体的可变区与T细胞淋巴瘤的T细胞受体的可变区,这些T细胞淋巴瘤在一些实施方案中也用作自身免疫疾病的靶抗原。其它肿瘤相关多肽可用作靶多肽,例如在肿瘤细胞中较高水平发现的多肽,包括单克隆抗体17-1A所识别的多肽和叶酸结合多肽。
其它合适的治疗性多肽和蛋白质包括通过赋予抵御自身免疫相关靶的宽基础保护性免疫应答来治疗患自身免疫疾病和紊乱的个体的多肽和蛋白质,所述靶包括细胞受体和产生“自我定向抗体”的细胞。T细胞介导的自身免疫疾病包括类风湿性关节炎(RA)、多发性硬化症(MS)、Sjgren综合征、结节病、胰岛素依赖型糖尿病(IDDM)、自身免疫甲状腺炎、反应性关节炎、强直性脊柱炎、硬皮病、多肌炎、皮肌炎、银屑病、脉管炎、韦格纳肉芽肿病、克罗恩病和溃疡性结肠炎。这些疾病中的每种均以与内源性抗原结合并启动自身免疫疾病相关的炎性级联反应的T细胞受体(TCR)为特征。
C.免疫原性转基因
本发明AAV载体适当地避免产生针对包含于载体中的AAV序列的免疫应答。然而,这些载体的配制方式可能是使得载体携带的转基因表达以诱导对所选抗原的免疫应答。例如,为促进免疫应答,转基因可从组成型启动子表达,给载体加入本文所述辅助载体和/或可将载体放进退化组织中。
例如,合适的免疫原性转基因可选自各种病毒科。需要免疫应答来抵御所需病毒科的例子包括导致约50%病例的普通感冒的鼻病毒属的小RNA病毒科;包括脊髓灰质炎病毒、柯萨奇病毒、艾柯病毒和人肠病毒(如甲肝病毒)的肠病毒属;与主要在非人灵长类中导致口蹄疫的口疮病毒属。在小RNA病毒科内,靶抗原包括VP1、VP2、VP3、VP4和VPG。其它病毒科包括星状病毒和杯状病毒科。杯状病毒科包括作为流行性肠胃炎重要病因的病毒的诺瓦克群。另一种希望用于靶抗原在人或非人动物中诱导免疫应答的病毒科是披膜病毒科,该科包括甲型病毒属,该病毒属包括辛德毕斯病毒、罗斯河病毒、和委内瑞拉、东部和西部马脑炎病毒和风疹病毒属,包括风疹病毒。黄病毒科包括登革热、黄热、日本脑炎、圣路易斯脑炎和蜱传性脑炎病毒。其它靶抗原可从丙型肝炎或冠状病毒科产生,其中包括许多非人病毒,例如感染性支气管炎病毒(家禽)、猪传染性胃肠病毒(猪)、猪凝血性脑脊髓炎病毒(猪)、猫感染性腹膜炎病毒(猫)、猫肠冠状病毒(猫)、犬冠状病毒(犬)和可导致普通感冒和/或非-甲型、乙型或丙型肝炎的人呼吸冠状病毒并且包括严重急性呼吸道综合症(SARS)的假定病因。在冠状病毒科内,靶抗原包括E1(也称为M或基质蛋白)、E2(也称为S或Spike蛋白)、E3(也称为HE或血凝素-依尔替糖)、糖蛋白(不存在于所有的冠状病毒中)或N(核衣壳)。其它抗原可靶向抵御动脉炎病毒科和弹状病毒科,弹状病毒科包括水疱病毒属(例如,疱疹口炎病毒)和狂犬病病毒属(例如,狂犬病)。在弹状病毒科内,合适的抗原来源于G蛋白或N蛋白。包括出血热病毒,例如马尔堡和埃博拉病毒的丝状病毒科是合适的抗原来源。副黏病毒科包括1型副流感病毒、3型副流感病毒、3型牛副流感病毒、腮腺炎病毒(流行性腮腺炎病毒)、2型副流感病毒、4型副流感病毒、新城疫病毒(小鸡)、牛瘟病毒、麻疹病毒,包括麻疹和犬瘟热,和肺病毒,包括呼吸道合胞病毒。流感病毒分类在正黏病毒科内并且是抗原(例如,HA蛋白、N1蛋白)的合适来源。布尼亚病毒科包括布尼亚病毒属(加利福尼亚脑炎,拉克罗斯脑炎)、白蛉热病毒属(裂谷热)、汉坦病毒属(普马拉是hemahagin热病毒)、内罗毕病毒属(内罗毕绵羊病)和各种未命名的银环蛇病毒(bungaviruses)。沙粒病毒科提供抗LCM和拉沙热病毒的抗原来源。抗原的另一来源是博内病毒科。呼肠孤病毒科包括呼肠孤病毒属、轮状病毒属(导致儿童急性肠胃炎)、环状病毒属和科罗拉多蜱热病毒属(科罗拉多蜱热病毒属、Lebombo病毒属(人)、马变性脑病病毒属、蓝舌病病毒属)。逆转录病毒科包括RNA肿瘤病毒亚科,该亚科包括人和兽医疾病,例如猫白血病病毒、HTLVI和HTLVII、慢病毒亚科(包括HIV、猿免疫缺陷病毒、猫免疫缺陷病毒、犬感染性贫血病毒和泡沫病毒亚科)。乳多空病毒科包括多瘤病毒亚科(BKU和JCU病毒)和乳头瘤病毒亚科(与癌症或乳头瘤的恶性进展有关)。腺病毒科包括导致呼吸疾病和/或肠炎的病毒(EX、AD7、ARD、O.B.)。细小病毒科包括猫细小病毒(猫肠炎)、猫全白细胞减少病病毒、犬细小病毒和猪细小病毒科。疱疹病毒科包括α疱疹病毒亚科,该亚科包括单纯疱疹病毒属(HSVI、HSVII)、水痘病毒属(假狂犬病、水痘-带状疱疹);β疱疹病毒亚科,该亚科包括巨细胞病毒属(HCMV,鼠巨细胞病毒属);和γ疱疹病毒亚科,该亚科包括淋巴潜伏病毒属、EBV(伯基特淋巴瘤)、人疱疹病毒6A、6B和7、卡波西肉瘤相关疱疹病毒与弥猴疱疹病毒(B病毒)、传染性鼻气管炎病毒属、马雷克病病毒属和细长病毒属。痘病毒科包括脊椎动物痘病毒亚科,包括正痘病毒属(大天花(天花)和牛痘(牛痘))、副痘病毒属、禽痘病毒属、山羊痘病毒属、兔痘病毒属、猪痘病毒属和昆虫痘病毒亚科。嗜肝DNA病毒科包括乙肝病毒。可是抗原的合适来源的一种未分类病毒是肝炎δ病毒、戊肝病毒和朊病毒。另一种抗原来源的病毒是Nipan病毒。其它的病毒来源包括鸟感染性粘液囊病病毒和猪呼吸和生殖综合征病毒。甲型病毒科包括马动脉炎病毒和各种脑炎病毒。
本发明也包括用于免疫人或非人动物抵御其它病原体的免疫原,所述病原体包括细菌、真菌、寄生微生物或感染人和非人脊椎动物的多细胞寄生虫、或来自癌细胞或肿瘤细胞的病原体。细菌病原体的例子包括致病性革兰氏阳性球菌,包括肺炎球菌、葡萄球菌(与其产生的毒素,例如肠毒素B)和链球菌。致病性革兰氏阴性球菌包括脑膜炎球菌、淋球菌。致病性肠革兰氏阴性杆菌包括肠杆菌科;假单胞菌属、不动杆菌属和埃肯菌属;类鼻疽假单孢菌属;沙门氏菌属;志贺氏菌属;嗜血杆菌属;莫拉氏菌属;杜克雷嗜血杆菌(H.ducreyi)(导致软下疳);布鲁杆菌(布鲁杆菌病);野兔热弗朗西丝菌(Franisella tularensis)(导致兔热病);鼠疫耶尔森菌(鼠疫)和其它耶尔森菌属(巴斯德菌属);念珠状链杆菌和螺菌属;革兰氏阳性杆菌,包括单核细胞增多李斯特菌;红斑丹毒丝菌;白喉棒状杆菌(Corynebacteriumdiphtheria)(白喉);霍乱;炭疽杆菌(B.anthracis)(炭疽);多诺万病(腹股沟肉芽肿)和巴尔通体病。致病性厌氧细菌导致的疾病包括破伤风、肉毒中毒(肉毒梭菌(Clostridum botulimum)及其毒素);产气荚膜梭菌(Clostridium perfringens)及其ε毒素;其它梭菌属;结核病、麻风和其它分枝杆菌属。致病性螺旋体疾病包括梅毒;密螺旋体病:雅司病、品他病和地方性梅毒;以及钩端螺旋体病。较高致病性细菌和致病性真菌导致的其它感染包括鼻疽(鼻疽伯克霍尔德氏菌(Burkholderiamallei);放线菌病;诺卡菌病;隐球菌病、芽生菌病、组织胞浆菌病和球孢子菌病;念珠菌病、曲霉病和毛霉病;孢子丝菌病;副球孢子菌病、石样真菌病、球拟酵母病、足分枝菌病和着色真菌病;和皮真菌病。立克次体感染包括斑疹伤寒热、落矶山斑疹热、Q热(伯内特科克斯立克次体(Coxiella burnetti))和立克次氏体痘。支原体和衣原体感染的例子包括:支原体肺炎、性病性淋巴肉芽肿、鹦鹉热和围产期衣原体感染。致病性真核细胞包括致病性原生动物和蠕虫并且由其产生的感染包括:阿米巴病、疟疾、利什曼病、锥虫病、弓形体病、卡氏肺囊虫(Pneumocystis carinii)、Trichans、鼠弓形体(Toxoplasma gondii)、巴贝虫病、贾第虫病、旋毛虫病、丝虫病、血吸虫病、线虫、吸虫(trematodes)或吸虫(flukes)感染和绦虫(绦虫)感染。
作为具有用于生物攻击潜力的制剂的许多这些生物体和/或其产生的毒素由疾病控制中心[(CDC),健康与人类服务部(Department of Heath and Human Services),美国]鉴定。例如,一些生物制剂,包括现在分类为A类制剂的炭疽杆菌(Bacillusanthracis)(炭疽)、肉毒梭菌(Clostridium botulinum)及其毒素(肉毒中毒)、鼠疫耶尔森菌(Yersinia pestis)(鼠疫)、大天花(天花)、野兔热弗朗西丝菌(Franisellatularensis)(兔热病)和病毒性出血热[丝状病毒(例如,埃博拉病毒、马尔堡病毒)和沙粒病毒[例如,拉沙病毒、马丘博病毒]];现在分类为B类制剂的伯内特科克斯立克次体(Coxiella burnetti)(Q热)、布鲁杆菌(布鲁杆菌病)、鼻疽伯克霍尔德氏菌(Burkholderia mallei)(鼻疽)、假鼻疽伯克霍尔德氏菌(Burkholderia pseudomallei)(类鼻疽)、蓖麻(Ricinus communis)及其毒素(蓖麻蛋白毒素)、产气荚膜梭菌(Clostridiumperfringens)及其毒素(ε毒素)、葡萄球菌(Staphylococcus)及其毒素(肠毒素B)、鹦鹉热衣原体(Chlamydia psittaci)(鹦鹉热)、水上安全恐吓(water safety threats)(例如,霍乱弧菌(Vibrio cholerae)、小隐孢子虫(Cryptosporidium parvum)、斑疹伤寒热(普氏立克次体(Richettsia powazekii))和病毒性脑炎(甲型病毒,例如委内瑞拉马脑炎、东部马脑炎、西部马脑炎);和现在分类为C类制剂的Nipan病毒和汉坦病毒。此外,其它如此分类或不同地分类的生物体在将来可鉴定和/或用于这种目的。容易理解的是,文中所述的病毒载体和其它构建物可用于传递这些生物体的抗原、病毒、其毒素或其它副产物,这些物质可预防和/或治疗感染或使用这些生物制剂产生的其它不良反应。
施用本发明的载体来递送抗T细胞可变区的免疫原引发包括CTL的免疫应答来消除T细胞。在类风湿性关节炎(RA)中已鉴定了参与疾病的TCR的几种特定可变区。这些TCR包括V-3、V-14、V-17和V-17。因此,递送至少编码这些多肽的一种的核酸序列引发靶向参与RA的T细胞的免疫应答。在多发性硬化症(MS)中已鉴定了参与该疾病的TCR的几种特定的可变区。这些TCR包括V-7和V-10。因此,递送至少编码这些多肽的一种的核酸序列引发靶向参与MS的T细胞的免疫应答。在硬皮病中已鉴定了参与疾病的TCR的几种特定可变区。这些TCR包括V-6、V-8、V-14和V-16、V-3C、V-7、V-14、V-15、V-16、V-28和V-12。因此,递送至少编码这些多肽的一种的核酸分子引发靶向参与硬皮病的T细胞的免疫应答。
因此,本发明的rAAV衍生的病毒载体提供足够的基因传送载体,这些载体能在体内或离体,甚至是生物体具有针对一种或多种AAV来源的中和抗体的情况下将所选的转基因递送至所选的宿主细胞。在一个实施方案中,rAAV与细胞离体混合,使用常规方法培养感染的细胞,将转导的细胞重新注入患者。
这些组合物尤其适用于治疗目的和免疫接种的基因递送,包括诱导保护性免疫。此外,本发明的组合物也可用于在体外产生所需基因产物。就体外生产而言,所需产物(例如,蛋白质)可在用含有编码所需产物的分子的rAAV转染宿主细胞,并在允许表达的条件下培养细胞培养物后从所需培养物中获得。然后,可视需要纯化和分离该表达产物。合适的转染、细胞培养、纯化和分离技术是本领域技术人员已知的。
以下实施例阐述了本发明的几个方面与实施方案。
实施例1-灵长类AAV序列的计算分析
A.收集灵长类组织
非人灵长类组织的来源已得到描述[N.Muzyczka,K.I.Berns,刊于《病毒学领域》(Fields Virology),D.M.Knipe,P.M.Howley编(Lippincott Williams&Wilkins,费城,2001),第2卷,2327-2359页]。通过两个主要的国家人组织供应方,合作人类组织网(Cooperative Human Tissue Network)(CHTN)和国家疾病研究交流协会(National Disease Research Interchange)(NDRI)从手术过程中或尸检或器官供者收集人组织。用于此项研究的人组织由18种不同的组织类型组成,包括结肠、肝、肺、脾、肾、脑、小肠、骨髓、心脏、淋巴结、骨骼肌、卵巢、胰腺、胃、食道、子宫颈、睾丸和前列腺。组织样品来自不同性别、种族(高加索人、非洲-美洲人、亚洲-西班牙人)和年龄(23-86岁)组的个体。在来自250个人体的259份分析样品中,约28%的组织与病理学相关。
B.AAV序列的检测与分离
如前所述,总的细胞DNA从人和非人灵长类组织中提取[R.W.Atchison等,Science 194,754-756(1965)]。使用引物和类似于用在非人灵长类分析中的条件通过签名(signature)或全长cap PCR确定AAV在人中的分子优势和组织分布。在所选人组织的AAV分离物中采用与非人灵长类AAV的扩展家族的分离和鉴定使用的相同PCR克隆方案。简言之,通过PCR和Topo-克隆(Invitrogen)从组织DNA扩增含有部分rep和全长cap序列的3.1kb片段。人AAV克隆最初通过限制酶切作图分析来协助鉴定AAV序列的多样性,然后通过精度达99.9%的SeqWright(SeqWright,休斯顿,得克萨斯)进行全序列分析。鉴定了总共67个分离自人组织的衣壳克隆(hu.1-hu.67)。86个非人灵长类组织的cap克隆得到测序,其中70个克隆来自恒河猴、6个克隆来自南美弥猴、3个克隆来自豚尾弥猴、2个克隆来自狒狒、5个克隆来自黑猩猩。
C.AAV序列的分析
分析了所有毗连序列的AAV衣壳病毒蛋白(vp1)开放读框(ORF)。用ClustalX1.81TM程序[H.D.Mayor,J.L.Melnick,Nature 210,331-332(1966)]对比AAV衣壳vp1蛋白序列,用BioEditTM[U.Bantel-Schaal,H.Zur Hausen,Virology 134,52-63(1984)]软件包产生框内DNA对比。用MEGATM2.1版和TreePuzzleTM软件包推断系统发育情况。使用邻接、最大简约性和最大可能性[M.Nei,S.Kumar,《分子进化和系统发生学》(Molecular Evolution and Phylogenetics)(牛津大学出版社,纽约,2000);H.A.Schmidt,K.Strimmer,M.Vingron,A.von Haeseler,Bioinformatics18,502-4(2002年3月);N.Saitou,M.Nei,Mol Biol Evol 4,406-25(1987年7月)]算法来确认单族组中类似的序列成簇。
然后从所有蛋白质序列的邻接系统发生树确定进化支。使用泊松-校正估计氨基酸距离。用1000个复制品进行自引分析。当序列具有0.05遗传距离内的连接节点时,可认为它们是单族的。3个或更多来源的一组序列认为是1个进化支。还通过序列分析评估了AAV种系发生的重组证据。执行裂口分解算法[H.J.Bandelt,A.W.Dress,Mol Phylogenet Evol 1,242-52(1992年9月)]筛选相似性。然后用Simplot软件中的Bootscan算法[M.Nei和S.Kumar,《分子进化和系统发生学》(MolecularEvolution and Phylogenetics)(牛津大学出版社,纽约,2000)]进一步分析以该方式获取的裂口的重组情况。使用400个核苷酸的滑动窗口(10个核苷酸/步)获得100自引复制邻接树。然后,从假定的重组片段推断裂口分解和邻接系统发生。自引值的明显提高、裂口的降低和杂交序列与其亲代来源的再分组可认为是重组的标准。
扩增自8个不同人对象的许多不同cap序列显示在Cap DNA序列的位置1400处的常见断点周围与AAV2(5’)和AAV3(3’)的系统发生关系,这与杂交病毒的重组和形成一致。从恒河猴的肠系膜淋巴结分离物检测到重组是在cap基因的通用区域[Gao等,Proc Natl Acad Sci美国100,6081-6086(2002年5月13日)]。执行Neib-Gofobori方法选择基于Z-测试的全部密码子[R.M.Kotin,Hum Gene Ther 5,793-801(1994年7月)]。
再次进行排除阳性鉴定为杂种的克隆的系统发生分析。在该项分析中包括鹅与鸟AAV作为外类群(l.Bossis,J.A.Chiorini,J Virol 77,6799-810(2003年6月))。图1是邻接树;使用最大简约性和最大可能性分析得到了类似关系。
该项分析证明11个系统发生群(总结于表1)。6个AAV进化支和5个单独AAV克隆(或克隆的集合)的种来源以数字或在取样中回收序列的来源表示。括号内显示每种类和每分组收集的序列总数。前述每个进化支的代号显示于右侧一列。Rhesus-恒河猴;cyno-南美弥猴;chimp-黑猩猩;pigtail-豚尾弥猴。
表1
每个种类和每个进化支或克隆的来源(序列)数目的分类
进化支/代表 | 人 | Rhesus | Cyno | 狒狒 | Chimp | Pigtail |
A/AAV1(AAV6)B/AAV2C/AAV2-AAV3杂种D/AAV7E/AAV8F/AAV9克隆AAV3AAV4AAV5Ch.5Rh.8 | 3(4)12(22)8(17)7(9)3(3) | 5(10)7(16)1(3)2(2) | 5(5) | 1(2) | 1(1) | 1(3) |
如上所示,因为在所用的标准系统发生算法中未执行重组,为了建立合适的系统发生树,从该分析中排除其重组祖先已确定的序列。所有非重组序列的邻接分析与确实利用重组得到的进化支并排表示。使用不同的算法与作为输入的核苷酸序列产生了类似结果。
如以下实施例所述,进行了其它实验来评估系统发生与作为血清学活性和向性检测到的功能有亲缘关系。
实施例2-新的人AAV的血清学分析
如以上实施例所述,获得的最后一个进化支来源于3个人的分离物,并且不含有上述的血清型。产生了针对该进化支的代表性成员的多克隆抗血清并综合研究了上述血清型之间的血清学交叉反应性。该研究表明新的人进化支在血清学上不同于其它已知的血清型,因此称为进化支F(以AAV9表示)。
通过用每种AAV载体的1×1013基因组拷贝与等体积的不完全弗氏佐剂一起对动物进行肌肉内接种产生针对AAV血清型1-9的家兔多克隆抗体。在第34天再次注射以加强抗体滴度。通过评价家兔抗血清对携带报道基因(携带增强型绿色荧光蛋白的AAVCMVEGFP)的载体转导293细胞的抑制作用确定AAV 1-9之间的血清学交叉反应性,所述载体是具有来源于不同AAV源衣壳的假型。用紫外显微镜评价AAVCMVEGFP载体对84-31细胞的转导。在评价两种AAV之间的血清学关系中,测试了异源和同源血清中和每种AAV载体的能力。如果血清以交互方式中和异源载体的能力低于中和同源载体的至少16倍,则认为此两种AAV是不同的血清型。如上所述测定中和滴度(G.P.Gao等,Proc Natl Acad Sci美国99,11854-9(2002年9月3日))。
表2
新的AAV载体的血清学评价
用于中和测定的载体假型 | |||||||||
来自用以下物质免疫的家兔 | AAV2/1 | AAV2/2 | AAV2/3 | AAV2/4 | AAV2/5 | AAV2/6 | AAV2/7 | AAV2/8 | AAV2/9 |
AAV2/1 | 1/163,840 | No NAB | No NAB | No NAB | 1/40,960 | 1/40,960 | 1/40 | No NAB | No NAB |
AAV2/2 | 1/80 | 1/81,920 | 1/5,120 | 1/20 | No NAB | 1/80 | 1/40 | 1/40 | No NAB |
AAV2/3 | 1/1,280 | 1/2,560 | 1/40,960 | 1/20 | 1/40 | 1/2,560 | 1/1,280 | 1/1,280 | No NAB |
AAV2/4 | 1/20 | No NAB | No NAB | 1/1,280 | 1/40 | No NAB | No NAB | No NAB | 1/40 |
AAV2/5 | 1/20,480 | No NAB | 1/80 | No NAB | 1/163,840 | 1/5,120 | 1/40 | No NAB | No NAB |
AAV2/6 | 1/81,920 | No NAB | 1/640 | 1/40 | 1/40 | 1/327,680 | 1/40 | No NAB | 1/40 |
AAV2/7 | 1/1,280 | 1/640 | 1/1,280 | 1/20 | No NAB | 1/1,280 | 1/163,840 | 1/5,120 | 1/80 |
AAV2/8 | 1/20 | 1/1,280 | 1/1,280 | No NAB | 1/20 | No NAB | 1/640 | 1/327,680 | 1/2,560 |
AAV2/9 | No NAB | No NAB | No NAB | No NAB | No NAB | No NAB | 1/20 | 1/640 | 1/20,480 |
除了结构不同的AAV5和AAV1血清型的未预计的血清学反应性(即,在交互滴定中异源、同源滴度之比是1/4和1/8)之外,这些数据证实了不同克隆和进化支的系统发生分组。
该结果还表明AAVhu.14具有不同的血清学特性并且与产生自任何已知AAV血清型的抗血清无明显的交叉反应性。AAVhu.14独特的衣壳结构也证实了其血清学特殊性,其衣壳结构与所有其它在该研究中比较的AAV血清型共用低于85%的氨基酸序列。那些发现为我们将AAVhu.14命名为新的血清型,AAV9提供了基础。
实施例3-灵长类AAV作为基因传送载体的评价
通过产生假型载体研究了AAV的生物向性,该载体中表达GFP或分泌的报道基因α-1抗胰蛋白酶(A1AT)的重组AAV2基因组用来源于各种克隆和用于比较的每种灵长类AAV进化支的一个代表性成员的衣壳来包装。例如,得自AAV1的数据用于代表进化支A,然后AAV2代表进化支B、Rh.34代表AAV4、AAV7代表进化支D、AAV8代表进化支E、AAVHu.14代表进化支F。AAV5、AAVCh.5和AAVRh.8作为单独的AAV基因型进行比较。
基于GFP转导评价了载体的转导效率以及体内在肝、肌肉或肺中的转导效率(图4)。
A.体外
表达增强型绿色荧光蛋白(EGFP)的载体用于检测其在84-31细胞中的体外转导效率并用于研究它们的血清学特性。就功能分析而言,在84-31细胞中检测了不同的AAVCMVEGFP载体的体外转导,该细胞接种在96孔板中并以MOI为1×104GC每细胞用假型AAVCMVEGFP载体感染。用G.Gao等,Proc Natl Acad Sci美国99,11854-9(2002年9月3日)所述技术用具有AAV1、2、5、7、8和6个其它新AAV(Ch.5、Rh.34、Cy5、rh.20、Rh.8和AAV9)的衣壳假型AAV载体。相对EGFP转导效率以0、1、2和3评分,对应于感染48小时后用紫外显微镜估计的绿色细胞的0-10%、10-30%、30-70%和70-100%。
B.体内
就体内研究而言,人α-抗胰蛋白酶(A1AT)选为载体中灵敏且定量的报道基因并且在CMV-增强的小鸡β-肌动蛋白启动子控制下表达。使用CB启动子能获得高水平的组织非特异性和组成型A1AT基因传送,也能够利用同一载体制备物在感兴趣的任何组织中进行基因传送研究。用新的AAV载体(AAVCBhA1AT)以每只动物1×1011份基因组拷贝的剂量处理4-6周龄的NCR裸鼠,分别通过门静脉内、气管内和肌肉内注射进行肝、肺和肌肉的定向基因传送。基因传送后在不同的时间点收集血清样品,通过基于ELISA的试验测定A1AT浓度并相对于基因传送28天后不同的血清A1AT水平评分为0、1、2和3,评分取决于载体的施用途径(肝:0=A1AT<400ng/ml,1=A1AT 400-1000ng/ml,2=A1AT 1000-10,000ng/ml,3=A1AT>10,000ng/ml;肺:0=A1AT<200ng/ml,1=A1AT 200-1000ng/ml,2=A1AT1000-10,000ng/ml,3=A1AT>10,000ng/ml;肌肉:0=A1AT<100ng/ml,1=A1AT100-1000ng/ml,2=A1AT 1000-10,000ng/ml,3=A1AT>10,000ng/ml)。
人AAV,克隆28.4/hu.14(现在命名为AAV9)转导肝的效率类似于AAV8,转导肺的效率比AAV5好两个对数,转导肌肉的效率优于AAV1,而其它两个人克隆24.5和16.12(hu.12和hu.13)在所有3种靶组织中的性能勉强合格。克隆N721.8(AAVrh.43)在所有的3种组织中也表现出高性能。
为进一步分析AAV9和rh 43相比于肝(AAV8)、肺(AAV5)和肌肉(AAV1)候补标记的基因传送效率,进行了剂量应答实验。两种新的载体在肌肉中表现出至少比AAV1高10倍的基因传送(效率),在肝中的效率类似于AAV8而在肺中比AAV5高2个对数。
我们证明在所有3种组织中有足够基因传送效率的一组AAV,它们在每种组织中的效率类似或优于其候补标记。迄今为止,该种类中有3个新的AAV,两个来自恒河猴(rh10和43),一个来自人(hou.14或AAV9)。该3个AAV与它们的候补标记在鼠的肝、肺和肌肉中的相对基因传送效率的直接比较表明一些具有最佳适合度的灵长类AAV可能作为“超级”病毒衍生自严格的生物选择和进化。这些AAV很适用于基因传送。
C.生物学活性特征
根据基因传送效率,在一组克隆或进化支的成员内证明不同AAV的独特的生物学活性特征基本上一致。然而,体外转导不能预测体内基因传送的效率。基于转基因表达和差异的累积分析水平的相对得分开发了用于比较两种不同AAV假型之间生物学活性的算法。
根据下式计算每对AAV的体外和体内基因传送得分的累积差异并示于表中(ND=未确定)。载体A和B之间根据得分的累积功能差异=体外(A-B)+肺(A-B)+肝(A-B)+肌肉(A-B)。该数值越小,AAV之间的功能越相似。在(图中)灰色区域,序列的百分比差异以粗体斜体字表示。通过将成对删除缺口后的氨基酸差异数除以750(VP1蛋白序列对比的长度)来确定cap结构中的百分比差异。
这些研究指出了许多与人中细小病毒研究相关的组织。在广泛的人组织中普遍存在内源性AAV序列表明该病毒群的天然感染是很常见的。病毒序列的广泛组织分布和在肝、脾和肠道中频繁检测到表明传送是通过胃肠道进行并且病毒血症可能是感染的特征。
人和非人灵长类中序列的巨大多样性与向性和血清学功能性相关,表明多样性是由真实的生物压力,例如免疫逃逸驱动。明显,第二最常见的人进化支证明重组导致这种多样性,该进化支是上述两种AAV的杂交体。
系统发生分析的拓扑学检查揭示了病毒进化与其宿主限制之间的关系。整个依赖病毒属似乎衍生自鸟类AAV,这与Lukashov和Goudsmit(V.V.Lukashov,J.Goudsmit,J Virol 75,2729-40(2001年3月))发表的一致。AAV4和AAV5分离物早先分叉自其它AAV的后来发育。下一个重要的节将该种类分成两个主要的单族群。第一群有单独分离自人的克隆,包括进化支B、AAV3克隆、进化支C和进化支A;该群的种类限制的唯一例外是称为ch.5的黑猩猩的单一克隆。代表该属剩余成员的另一单族群来源于人和非人灵长类。该群包括从除弥猴以外的物种分离的进化支D和rh.8克隆与人特异性的进化支F。该群内的剩余进化支(即,进化支E)有来自人和许多非人灵长类的成员,说明该进化支的传播超越了种类障碍。有趣的是,分离自一些人的进化支E成员的衣壳结构与非人灵长类的一些基本相同,表明几乎未发生宿主适应。AAV8衍生载体的生物学分析证实了高水平的基因传送有宽范围的组织向性,这与更混杂的感染范围一致并且可以解释明显的人兽互通病。进化支F的基因传送的范围和效率甚至更大,增强了跨种传播的可能性,这是迄今为止未检测到的。
潜伏AAV在整个人和非人灵长类中广泛传播的存在及其明显的重组倾向与跨种障碍产生了重要的问题。重组可能导致出现有毒力改变的新传染物。尚待确定灵长类中AAV感染的临床后遗症的事实使得对这种可能性的评价复杂化。此外,AAV序列在肝中流行率高可能导致病毒通过同种和异种肝移植在人群中传播。最后,在人中发现内源性AAV涉及将AAV用于人基因治疗。野生型AAV在于灵长类中如此流行却未与恶性病变相关的事实说明它尤其不是致癌性的。事实上,AAVrep基因的表达显示抑制转化[P.L.Hermonat,Virology 172,253-61(1989年9月)]。
实施例4-用于治疗囊性纤维化气道疾病的AAV 2/9载体
迄今为止,传送至肺用于治疗CF气道疾病的CFTR基因受限于不佳的载体性能与气道上皮细胞造成有效的基因传送的明显障碍。包装于AAV9衣壳中的AAV2基因组(AAV2/9)在各种气道模型系统中与AAV2/5比较。
AAV2/9的50μl单一剂量的1×1011基因组拷贝(gc)鼻内滴注入裸鼠和C57B1/6小鼠,该AAV2/9基因组表达在小鸡β-肌动蛋白启动子的转录控制下核靶向β-半乳糖苷酶(nLacZ)基因或绿色荧光蛋白(GFP)基因。21天后,处理肺和鼻以表达基因。用AAV2/9-GFP转导对照动物,未见LacZ阳性细胞。AAV2/9-nLacZ主要经气道成功地转导,AAV2/5-nLacZ主要经肺泡和几乎不经气道转导。AAV2/5和AAV2/9均穿越鼻气道上皮转导纤毛和非纤毛上皮细胞。
现在评价了上皮细胞特异性启动子来改善体内靶向气道细胞。然后,基于体内发现测试了AAV2/9对人气道上皮细胞的基因传送效率。气道上皮细胞分离自人气管和支气管并生长在胶原包被的膜支持物上的气-液-界面(ALI)处。一旦细胞极化与分化,用表达GFP的AAV2/9或AAV2/5从尖端和基底外侧转导它们。AAV2/5和AAV2/9成功地从基底外侧表面转导上皮细胞。然而,当应用于尖端表面时,AAV2/9转导的细胞数目高于AAV2/5的10倍。现在评价了AAV2/9在非人灵长类的肺与鼻气道中的基因传送效率。
该实验证实,AAV2/9可有效地转导鼠肺的气道和生长于ALI中的良好分化的人气道上皮细胞。
实施例5-AAV1(2/1)和AAV9(2/9)直接注射入成年大鼠心脏的比较
向两只成年大鼠(3月大)的左心室注射5×1011个AAV2/1或AAV2/9颗粒一次。
结果是惊人的,如lacZ组织化学方法所评价的,成年大鼠心脏中观察到AAV2/9载体表达明显高于AAV2/1。AAV2/9在新生小鼠心脏中也显示出更好的基因传送效率。
实施例6-用于B型血友病基因治疗的AAV2/9载体
该研究中,AAV2/9载体显示是用于B型血友病的肝和肌肉定向基因治疗中比传统的AAV来源更有效且更低免疫原性载体。
就肝定向方法而言,在小鼠和狗中血友病模型中评价AAV2/9假型载体。在免疫活性B型血友病小鼠(在C57BL/6背景中)中,以1×1011份基因组拷贝(GC)/小鼠门静脉内注射AAV2/7、2/8和2/9载体后实现了犬因子IX(cFIX,41-70μg/ml)的长期超生理水平与缩短的活化的部分凝血致活酶时间(aPTT),其中cFIX在肝特异性启动子(LSP)与旱獭乙肝转录后响应性元件(WPRE)控制下表达。剂量低10倍(1×1010GC/小鼠)的AAV2/8载体产生了正常水平的cFIX和aPTT时间。在北卡罗来纳大学(University of North Carolina)(UNC)B型血友病狗中,证实了将AAV2/8载体施用进已用AAV2载体处理过的狗中是成功的;第二次门静脉内注射(剂量=5×1012GC/kg)后的第6天达到表达cFIX峰值(10μg/ml),然后逐渐降低并在整个研究(1.5年)中稳定于700ng/ml左右(正常水平的16%)。该水平比相似剂量接受单次注射AAV2-cFIX的B血友病狗约高3倍。近来,以5.25×1012GC/kg剂量对两只首次用于实验的B型血友病狗门静脉内注射AAV2/8载体。注射10周后,一只狗(雄性)的cFIX水平达到正常水平的30%(1.5μg/ml)并维持于1.3-1.5μg/ml,而第二只狗(雌性)的cFIX表达约维持于正常水平的约10%。注射后,全血凝结时间(WBCT)和aPTT均缩短了,这说明抗原是生物活性的。手术后,两只狗中的肝酶(天冬氨酸转氨酶(SGOT))、丙氨酸转氨酶(SGPT)维持于正常水平。也评价了这些AAV用于B型血友病的肌肉定向基因治疗。以1×1011GC/小鼠的剂量肌肉内注射后,在免疫活性B型血友病小鼠(在C57BL/6背景中)中比较了用6种不同AAV来源包装的AAV-CMV-cFIX-WPRE[携带在CMV启动子控制下的cFIX并含有WPRE的AAV]。监测cFIX基因表达与抗体形成。在用AAV2/8载体注射的小鼠的血浆中检测到最高表达(在第42天为1460±392ng/ml),然后是注射AAV2/9(在第42天为773±171ng/ml)和注射AAV2/7的(在第42天为500±311ng/ml)。这些水平维持了5个月。令人意外的是,用AAV2/1的cFIX表达量的范围是0-253ng/ml(平均值:66±82ng/ml)。在一些AAV2/1注射的小鼠中检测到抗-cFIX抑制剂(IgG)。这些小鼠中的cFIX表达水平良好地与抑制剂水平相关。第28天的样品对所有AAV进行了进一步筛选抑制剂的形成。B型血友病小鼠显示针对AAV2/2的抑制剂形成最高,然后是AAV2/5和AAV2/1。注射AAV2/7、AAV2/8和AAV2/9的动物中仅检测到有个别和低水平的抑制剂。因此,用于B型血友病肌肉定向基因治疗的新AAV血清型2/9载体的优点是更有效且安全的载体而不引发任何明显的抗FIX抗体形成。
实施例7-本发明的新Rh.43载体
A.基于AAVrh.43的A1AT表达载体与AAV8和AAV9在小鼠肝脏定向基因传送中的比较
比较属于进化支E(通过系统发生分析)的新AAVrh.43载体与AAV8和新AAV9在门静脉内输注入小鼠肝脏后的hA1AT水平。更具体地说,在小鼠肝脏定向基因传送中比较了假型AAVrh.43、AAV2/8和AAV2/9载体。以1×1011GC、3×1010GC与1×1010GC/动物的剂量向4-6周龄C57BL/6小鼠肌肉内施用假型载体。在载体输注后第28天从动物中收集血清样品用于人α1抗胰蛋白酶(hA1AT)测定。
数据表明新AAVrh.43载体在小鼠模型中实际上与AAV9性能类似。
B.假型AAV载体介导的核靶向LacZ基因传送至小鼠肝脏和肌肉
比较了本发明新的基于AAV9和AAVrh.43的载体与基于AAV1和AAV2的载体。以1×1011GC/小鼠的剂量将载体经门静脉内注射到靶肝脏或肌肉内注射到C57BL/6小鼠的右胫前肌肉中。基因传送后第28天处死小鼠并收集感兴趣的组织进行X-gal组织化学染色。
证实AAVrh.43载体的基因传送效率接近AAV9但高于AAV1至少5倍。还用核靶向LacZ基因进一步分析了AAVrh.43在肝脏和肌肉中的特性,所述基因是作为报道基因用组织化学方法目测基因传送的程度。
C.基于AAVrh.43的A1AT表达载体与AAV5在小鼠肺定向基因传送中的比较
本发明新的基于rh.43的载体在肺组织中也表现出极好的基因传送能力。经气管内以不同剂量(1×1010、3×1010和1×1011GC/动物)向4-6周龄C57BL/6小鼠的肺部施用假型载体。于不同时间点收集小鼠的血清样品用于hA1AT测定。
比较不同剂量的该载体与AAV5在气管内滴注入小鼠肺部后hA1AT的水平。数据显示该新的载体在小鼠模型中比AAV5至少更有效100倍。
实施例8-用于肝脏和肺定向基因传送的新的基于人AAV的载体
人克隆AAVhu.37、AAVhu.41和AAVhu.47制成假型并检测在小鼠组织中的基因传送能力。使用本文所述的方法制备具有hu.37、hu.41和hu.47衣壳的假型AAVCBA1AT载体并经门静脉内与气管内注射施用至4-6周龄的C57BL/6小鼠。载体注射后第14天收集小鼠的血清样品用于按照公开的技术进行的hA1AT测定。AAVhu.47属于AAV2家族(进化支B)并且分离自人骨髓样品。AAVhu.37和AAVhu.41分别来自人睾丸组织和人骨髓样品。在系统发生学上,它们均属于AAV8进化支(进化支E)。
注射的动物的血清A1AT分析表明,AAV hu.41和AAV hu.47在3种测试的组织中表现不佳。然而,得自AAVhu.37的载体的基因传送能力类似于AAV8在肝脏和AAV9在肺中的。
本说明书中引用的所有出版物引为参考。尽管参考特别优选的实施方案描述了本发明,应该理解的是可做出改进而不脱离本发明的构思。
序列表
<110>宾夕法尼亚州立大学托管会(The Trustees of the University of Pennsylvania)
J.M.威尔森(Wilson,James M.)
G.高(Gao,Guangping)
M.R.阿尔维拉(Alvira,Mauricio R.)
L.H.范登伯格(Vandenberghe,Luk H.)
<120>腺伴随病毒(AAV)进化支、序列、含有这些序列的载体及它们的应用
<130>UPN-P3230PCT
<150>US 60/508,226
<151>2003-09-30
<150>US 60/566,546
<151>2004-04-29
<160>236
<170>PatentIn version 3.3
<210>1
<211>2211
<212>DNA
<213>腺伴随病毒,克隆hu.31
<400>1
atggctgccg atggttatct tccagattgg ctcgaggaca accttagtga aggaattcgc 60
gagtggtggg ctttgaaacc tggagcccct caacccaagg caaatcaaca acatcaagac 120
aacgctcgag gtcttgtgct tccgggttac aaataccttg gacccggcaa cggactcgac 180
aagggggagc cggtcaacgc agcagacgcg gcggccctcg agcacgacaa ggcctacgac 240
cagcagctca aggccggaga caacccgtac ctcaagtaca accacgccga cgccgagttc 300
caggagcggc tcaaagaaga tacgtctttt gggggcaacc tcgggcgagc agtcttccag 360
gccaaaaaga ggcttcttga acctcttggt ctggttgagg aagcggctaa gacggctcct 420
ggaaagaaga ggcctgtaga gcagtctcct caggaaccgg actcctccgc gggtattggc 480
aaatcgggtg cacagcccgc taaaaagaga ctcaatttcg gtcagactgg cgacacagag 540
tcagtcccag accctcaacc aatcggagaa cctcccgcag ccccctcagg tgtgggatct 600
cttacaatgg cttcaggtgg tggcgcacca gtggcagaca ataacgaagg tgccgatgga 660
gtgggtagtt cctcgggaaa ttggcattgc gattcccaat ggctggggga cagagtcatc 720
accaccagca cccgaacctg ggccctgccc acctacaaca atcacctcta caagcaaatc 780
tccaacagca catctggagg atcttcaaat gacaacgcct acttcggcta cagcaccccc 840
tgggggtatt ttgacttcaa cagattccac tgccacttct caccacgtga ctggcagcga 900
ctcatcaaca acaactgggg attccggcct aagcgactca acttcaagct cttcaacatt 960
caggtcaaag aggttacgga caacaatgga gtcaagacca tcgccaataa ccttaccagc 1020
acggtccagg tcttcacgga ctcagactat cagctcccgt acgtgctcgg gtcggctcac 1080
gagggctgcc tcccgccgtt cccagcggac gttttcatga ttcctcagta cgggtatctg 1140
acgcttaatg atggaagcca ggccgtgggt cgttcgtcct tttactgcct ggaatatttc 1200
ccgtcgcaaa tgctaagaac gggtaacaac ttccagttca gctacgagtt tgagaacgta 1260
cctttccata gcagctacgc tcacagccaa agcctggacc gactaatgaa tccactcatc 1320
gaccaatact tgtactatct ctcaaagact attaacggtt ctggacagaa tcaacaaacg 1380
ctaaaattca gtgtggccgg acccagcaac atggctgtcc agggaagaaa ctacatacct 1440
ggacccagct accgacaaca acgtgtctca accactgtga ctcaaaacaa caacagcgaa 1500
tttgcttggc ctggagcttc ttcttgggct ctcaatggac gtaatagctt gatgaatcct 1560
ggacctgcta tggccagcca caaagaagga gaggaccgtt tctttccttt gtctggatct 1620
ttaatttttg gcaaacaagg aactggaaga gacaacgtgg atgcggacaa agtcatgata 1680
accaacgaag aagaaattaa aactactaac ccggtagcaa cggagtccta tggacaagtg 1740
gccacaaacc accagagtgc ccaagcacag gcgcagaccg gctgggttca aaaccaagga 1800
atacttccgg gtatggtttg gcaggacaga gatgtgtacc tgcaaggacc catttgggcc 1860
aaaattcctc acacggacgg caactttcac ccttctccgc tgatgggagg gtttggaatg 1920
aagcacccgc ctcctcagat cctcatcaaa aacacacctg tacctgcgga tcctccaacg 1980
gccttcaaca aggacaagct gaactctttc atcacccagt attctactgg ccaagtcagc 2040
gtggagatcg agtgggagct gcagaaggaa aacagcaagc gctggaaccc ggagatccag 2100
tacacttcca actattacaa gtctaataat gttgaatttg ctgttaatac tgaaggtgta 2160
tatagtgaac cccgccccat tggcaccaga tacctgactc gtaatctgta a 2211
<210>2
<211>2211
<212>DNA
<213>新AAV清型,克隆hu.32
<400>2
atggctgccg atggttatct tccagattgg ctcgaggaca ctctctctga aggaataaga 60
cagtggtgga agctcaaacc tggcccacca ccaccaaagc ccgcagagcg gcataaggac 120
gacagcaggg gtcttgtgct tcctgggtac aagtacctcg gacccggcaa cggactcgac 180
aagggggagc cggtcaacgc agcagacgcg gcggccctcg agcacgacaa ggcctacgac 240
cagcagctca aggccggaga caacccgtac ctcaagtaca accacgccga cgccgagttc 300
caggagcggc tcaaagaaga tacgtctttt gggggcaacc tcgggcgagc agtcttccag 360
gccaaaaaga ggcttcttga acctcttggt ctggttgagg aagcggctaa gacggctcct 420
ggaaagaaga ggcctgtaga gcagtctcct caggaaccgg actcctccgc gggtattggc 480
aaatcgggtt cacagcccgc taaaaagaaa ctcaatttcg gtcagactgg cgacacagag 540
tcagtccccg accctcaacc aatcggagaa cctcccgcag ccccctcagg tgtgggatct 600
cttacaatgg cttcaggtgg tggcgcacca gtggcagaca ataacgaagg tgccgatgga 660
gtgggtagtt cctcgggaaa ttggcattgc gattcccaat ggctggggga cagagtcatc 720
accaccagca cccgaacctg ggccctgccc acctacaaca atcacctcta caagcaaatc 780
tccaacagca catctggagg atcttcaaat gacaacgcct acttcggcta cagcaccccc 840
tgggggtatt ttgacttcaa cagattccac tgccacttct caccacgtga ctggcagcga 900
ctcatcaaca acaactgggg attccggcct aagcgactca acttcaagct cttcaacatt 960
caggtcaaag aggttacgga caacaatgga gtcaagacca tcgccaataa ccttaccagc 1020
acggtccagg tcttcacgga ctcagactat cagctcccgt acgtgctcgg gtcggctcac 1080
gagggctgcc tcccgccgtt cccagcggac gttttcatga ttcctcagta cgggtatctg 1140
acgcttaatg atgggagcca ggccgtgggt cgttcgtcct tttactgcct ggaatatttc 1200
ccgtcgcaaa tgctaagaac gggtaacaac ttccagttca gctacgagtt tgagaacgta 1260
cctttccata gcagctacgc tcacagccaa agcctggacc gactaatgaa tccactcatc 1320
gaccaatact tgtactatct ctcaaagact attaacggtt ctggacagaa tcaacaaacg 1380
ctaaaattca gcgtggccgg acccagcaac atggctgtcc agggaagaaa ctacatacct 1440
ggacccagct accgacaaca acgtgtctca accactgtga ctcaaaacaa caacagcgaa 1500
tttgcttggc ctggagcttc ttcttgggct ctcaatggac gtaatagctt gatgaatcct 1560
ggacctgcta tggccagcca caaagaagga gaggaccgtt tctttccttt gtctggatct 1620
ttaatttttg gcaaacaagg aactggaaga gacaacgtgg atgcggacaa agtcatgata 1680
accaacgaag aagaaattaa aactactaac ccggtagcaa cggagtccta tggacaagtg 1740
gccacaaacc accagagtgc ccaagcacag gcgcagaccg gctgggttca aaaccaagga 1800
atacttccgg gtatggtttg gcaggacaga gatgtgtacc tgcaaggacc catttgggcc 1860
aaaattcctc acacggacgg caactttcac ccttctccgc taatgggagg gtttggaatg 1920
aagcacccgc ctcctcagat cctcatcaaa aacacacctg tacctgcgga tcctccaacg 1980
gctttcaata aggacaagct gaactctttc atcacccagt attctactgg ccaagtcagc 2040
gtggagattg agtgggagct gcagaaggaa aacagcaagc gctggaaccc ggagatccag 2100
tacacttcca actattacaa gtctaataat gttgaatttg ctgttaatac tgaaggtgta 2160
tatagtgaac cccgccccat tggcaccaga tacctgactc gtaatctgta a 2211
<210>3
<211>2211
<212>DNA
<213>腺伴随病毒,人克隆9
<400>3
atggctgccg atggttatct tccagattgg ctcgaggaca accttagtga aggaattcgc 60
gagtggtggg ctttgaaacc tggagcccct caacccaagg caaatcaaca acatcaagac 120
aacgctcgag gtcttgtgct tccgggttac aaataccttg gacccggcaa cggactcgac 180
aagggggagc cggtcaacgc agcagacgcg gcggccctcg agcacgacaa ggcctacgac 240
cagcagctca aggccggaga caacccgtac ctcaagtaca accacgccga cgccgagttc 300
caggagcggc tcaaagaaga tacgtctttt gggggcaacc tcgggcgagc agtcttccag 360
gccaaaaaga ggcttcttga acctcttggt ctggttgagg aagcggctaa gacggctcct 420
ggaaagaaga ggcctgtaga gcagtctcct caggaaccgg actcctccgc gggtattggc 480
aaatcgggtg cacagcccgc taaaaagaga ctcaatttcg gtcagactgg cgacacagag 540
tcagtcccag accctcaacc aatcggagaa cctcccgcag ccccctcagg tgtgggatct 600
cttacaatgg cttcaggtgg tggcgcacca gtggcagaca ataacgaagg tgccgatgga 660
gtgggtagtt cctcgggaaa ttggcattgc gattcccaat ggctggggga cagagtcatc 720
accaccagca cccgaacctg ggccctgccc acctacaaca atcacctcta caagcaaatc 780
tccaacagca catctggagg atcttcaaat gacaacgcct acttcggcta cagcaccccc 840
tgggggtatt ttgacttcaa cagattccac tgccacttct caccacgtga ctggcagcga 900
ctcatcaaca acaactgggg attccggcct aagcgactca acttcaagct cttcaacatt 960
caggtcaaag aggttacgga caacaatgga gtcaagacca tcgccaataa ccttaccagc 1020
acggtccagg tcttcacgga ctcagactat cagctcccgt acgtgctcgg gtcggctcac 1080
gagggctgcc tcccgccgtt cccagcggac gttttcatga ttcctcagta cgggtatctg 1140
acgcttaatg atggaagcca ggccgtgggt cgttcgtcct tttactgcct ggaatatttc 1200
ccgtcgcaaa tgctaagaac gggtaacaac ttccagttca gctacgagtt tgagaacgta 1260
cctttccata gcagctacgc tcacagccaa agcctggacc gactaatgaa tccactcatc 1320
gaccaatact tgtactatct ctcaaagact attaacggtt ctggacagaa tcaacaaacg 1380
ctaaaattca gtgtggccgg acccagcaac atggctgtcc agggaagaaa ctacatacct 1440
ggacccagct accgacaaca acgtgtctca accactgtga ctcaaaacaa caacagcgaa 1500
tttgcttggc ctggagcttc ttcttgggct ctcaatggac gtaatagctt gatgaatcct 1560
ggacctgcta tggccagcca caaagaagga gaggaccgtt tctttccttt gtctggatct 1620
ttaatttttg gcaaacaagg aactggaaga gacaacgtgg atgcggacaa agtcatgata 1680
accaacgaag aagaaattaa aactactaac ccggtagcaa cggagtccta tggacaagtg 1740
gccacaaacc accagagtgc ccaagcacag gcgcagaccg gctgggttca aaaccaagga 1800
atacttccgg gtatggtttg gcaggacaga gatgtgtacc tgcaaggacc catttgggcc 1860
aaaattcctc acacggacgg caactttcac ccttctccgc tgatgggagg gtttggaatg 1920
aagcacccgc ctcctcagat cctcatcaaa aacacacctg tacctgcgga tcctccaacg 1980
gccttcaaca aggacaagct gaactctttc atcacccagt attctactgg ccaagtcagc 2040
gtggagatcg agtgggagct gcagaaggaa aacagcaagc gctggaaccc ggagatccag 2100
tacacttcca actattacaa gtctaataat gttgaatttg ctgttaatac tgaaggtgta 2160
tatagtgaac cccgccccat tggcaccaga tacctgactc gtaatctgta a 2211
<210>4
<211>2217
<212>DNA
<213>新AAV血清型,克隆hu.17
<400>4
atggctgccg atggttatct tccagattgg ctcgaggaca acctctctga gggcattcgc 60
gagtggtggg acttgaaacc tggagccccg aaacccaaag ccaaccagca aaagcaggac 120
gacggccggg gtctggtgct tcctggctgc aagtacctcg gacccttcaa cggactcgac 180
aagggggagc ccgtcaacgc ggcggacgca gcggccctcg agcacgacaa ggcctacgac 240
cagcagctca aagcgggtga caatccgtac ctgcggtata accacgccga cgccgagttt 300
caggagcgtc tgcaagaaga tacgtctttt gggggcaacc tcgggcgagc agtcttccag 360
gccaagaagc gggttctcga acctctcggt ctggttgagg aaggcgctaa gacggctcct 420
ggaaagaaga gaccggtaga gccatcaccc cagcgttctc cagactcctc tacgggcatc 480
ggcaagacag gccagcagcc cgcgaaaaag agactcaact ttgggcagac tggcgactca 540
gagtcagtgc ccgaccctca accaatcgga gaaccccccg caggcccctc tggtctggga 600
tctggtacaa tggctgcagg cggtggcgct ccaatggcag acaataacga aggcgccgac 660
ggagtgggta gttcctcagg aaattggcat tgcgattcca catggctggg cgacagagtc 720
atcaccacca gcacccgaac ctgggccctc cccacctaca acaaccacct ctacaagcaa 780
atctccaacg ggacatcggg aggaagcacc aacgacaaca cctacttcgg ctacagcacc 840
ccctgggggt attttgactt taacagattc cactgccact tctcaccacg tgactggcag 900
cgactcatca acaacaactg gggattccgg cccaagagac tcaacttcaa gctcttcaac 960
atccaggtca aggaggtcac gcagaatgaa ggcaccaaga ccatcgccaa taaccttacc 1020
agcacgattc aggtctttac ggactcggaa taccagctcc cgtacgtcct cggctctgcg 1080
caccagggct gcccgcctcc gttcccggcg gacgtcttca tgattcctca gtacgggtac 1140
ctgactctga acaacggcag tcaggccgtg ggccgttcct ccttctactg cctggagtac 1200
tttccttctc aaatgcggag aacgggcaac aactttgagt tcagctacca gtttgaggac 1260
gtgccttttc acagcagcta cgcgcatagc caaagcctgg accggctgat gaaccccctc 1320
atcgaccagt acctgtacta cctgtctcgg actcagtcca cgggaggtac cgcaggaact 1380
cagcagttgc tattttctca ggccgggcct aataacatgt cggctcaggc caaaaactgg 1440
ctacccgggc cctgctaccg gcagcaacgc gtctccacga cactgtcgca aaataacaac 1500
agcaactttg cttggaccgg tgccaccaag tatcatctga atggcagaga ctctctggta 1560
aatcccggtg tcgctatggc aacgcacaag gacgacgaag agcgattttt tccatccagc 1620
ggagtcttga tgtttgggaa acagggagct ggaaaagaca acgtggacta tagcagcgtt 1680
atgctaacca gtgaggaaga aatcaaaacc accaacccag tggccacaga acagtacggc 1740
gtggtggccg ataacctgca acagcaaaac gccgctccta ttgtaggggc cgtcaacagt 1800
caaggagcct tacctggcat ggtctggcag aaccgggacg tgtacctgca gggtcctatc 1860
tgggccaaga ttcctcacac ggacggcaac tttcatcctt cgccgctgat gggaggcttt 1920
ggactgaaac acccgcctcc tcagatcctg attaagaata cacctgttcc cgcggatcct 1980
ccaactacct tcagtcaagc caagctggcg tcgttcatca cgcagtacag caccggacag 2040
gtcagcgtgg aaattgaatg ggagctgcag aaagagaaca gcaagcgctg gaacccagag 2100
attcagtata cttccaacta taacaaatct gttaatgtgg actttactgt ggacactaat 2160
ggtgtgtatt cagagcctcg ccccattggc accagatacc tgactcgtaa tctgtaa 2217
<210>5
<211>2217
<212>DNA
<213>新AAV血清型,克隆hu.6
<400>5
atggctgccg atggttatct tccagattgg ctcgaggaca acctctctga gggcattcgc 60
gagtggtggg acttgaaacc tggagccccg aaacccaaag ccaaccagca aaagcaggac 120
gacggccggg gtctggtgct tcctggctac aagtacctcg gacccttcaa cggactcgac 180
aagggggagc ccgtcaacgc ggcggacgca gcggccctcg agcacgacaa ggcctacgac 240
cagcagctca aagcgggtga caatccgtac ctgcggtata accacgccga cgccgagttt 300
caggagcgtc tgcaagaaga tacgtctttt gggggcaacc tcgggcgagc agtcttccag 360
gccaagaagc gggttctcga acctctcggt ctggttgagg aaggcgctaa gacggctcct 420
ggaaagaaga gaccggtaga gccatcaccc cagcgttctc cagactcctc tacgggcatc 480
ggcaagacag gccagcagcc cgcgaaaaag agactcaact ttgggcagac tggcgactca 540
gagtcagtgc ccgaccctca accaatcgga gaaccccccg caggcccctc tggtctggga 600
tctggtacaa tggctgcagg cggtggcgct ccaatggcag acaataacga aggcgccgac 660
ggagtgggta gttcctcagg aaattggcat tgcgattccg catggctggg cgacagagtc 720
atcaccacca gcacccgacc ctgggccctc cccacctaca acaaccacct ctacaagcaa 780
atctccaacg ggacatcggg aggaagcacc aacgacaaca cctacttcgg ctacagcacc 840
ccctgggggt attttgactt taacagattc cactgccact tctcaccacg tgactggcag 900
cgactcatca acaacaactg gggattccgg cccaagagac tcaacttcaa gctcttcaac 960
atccaggtca aggaggtcac gcagaatgaa ggcaccaaga ccatcgccaa taaccttacc 1020
agcacgattc aggtctttac ggactcggaa taccagctcc cgtacgtcct cggctctgcg 1080
caccagggct gcccgcctcc gttcccggcg gacgtcttca tgattcctca gtacgggtac 1140
ctgactctga acaacggcag tcaggccgtg ggccgttcct ccttctactg cctggagtac 1200
tttccttctc aaatgcggag aacgggcaac aactttgagt tcagctacca gtttgaggac 1260
gtgccttttc acagcagcta cgcgcatagc caaagcctgg accggctgat gaaccccctc 1320
atcgaccagt acctgtacta cctgtctcgg actcagtcca cgggaggtac cgcaggaact 1380
cagcagttgc tattttctca ggccgggcct aataacatgt cggctcaggc caaaaactgg 1440
ctacccgggc cctgctaccg gcagcaacgc gtctccacga cactgtcgca aaataacaac 1500
agcaactttg cttggaccgg tgccaccaag tatcatctga atggcagaga ctctctggta 1560
aatcccggtg tcgctatggc aacgcacaag gacgacgaag agcgattttt tccatccagc 1620
ggagtcttga tgtttgggaa acagggagct ggaaaagaca acgtggacta tagcagcgtt 1680
atgctaacca gtgaggaaga aatcaaaacc accaacccag tggccacaga acagtacggc 1740
gtggtggccg ataacctgca acagcaaaac gccgctccta ttgtaggggc cgtcaacagt 1800
caaggagcct tacctggcat ggtctggcag aaccgggacg tgtacctgca gggtcctatc 1860
tgggccaaga ttcctcacac ggacggcaac tttcatcctt cgccgctgat gggaggcttt 1920
ggactgaaac acccgcctcc tcagatcctg attaagaata cacctgttcc cgcggatcct 1980
ccaactacct tcagtcaagc caagctggcg tcgttcatca cgcagtacag caccggacag 2040
gtcagcgtgg aaattgaatg ggagctgcag aaagagaaca gcaagcgctg gaacccagag 2100
attcagtata cttccaacta ctacaaatct acaaatgtgg actttgctgt caatactgag 2160
ggtacttatt cagagcctcg ccccattggc acccgttacc tcacccgtaa cctgtaa 2217
<210>6
<211>2217
<212>DNA
<213>新AAV血清型,克隆hu.41
<400>6
atggctgctg acggttatct tccagattgg ctcgaggaca acctctctga gggcattcgc 60
gagtggtggg acctgaaacc tggagccccc aagcccaagg ccaaccagca gaagcaggac 120
gacggccggg gtctggtgct tcctggctac aagtacctcg gacccttcaa cggactcgac 180
aagggggagc ccgtcaacgc ggcggacgca gcggccctcg agcacgacaa ggcctacgac 240
cagcagctca aagcgggtga caatccgtac ctgcggtata accacgccga cgccgagttt 300
caggagcgtc tacaagaaga tacgtctttt gggggcaacc tcgggcgagc agtcttccag 360
gccaagaagc gggttctcga acctctcggt ccggttgagg aagctgctaa gacggctcct 420
ggaaagaaga gaccggtaga accgccacct cagcgttccc ccgactcctc cacgggcatc 480
ggcaagaaag gccagcagcc cgctaaaaag agactgaact ttggtcagac tggcgactca 540
gagtcagtcc ccgaccctca accaatcgga gaaccaccag caggcccctc tggtctggga 600
tctggtacaa tggctgcagg cggtggcgct ccaatggcag acaataacga aggcgccgac 660
ggagtgggta gttcctcagg aaattggcat tgcgattcca catggctggg cgacagagtc 720
atcaccacca gcacccgaac ctgggccctg cccacctaca acaaccacct ctacaagcaa 780
atatccaatg ggacatcggg aggaagcacc aacgacaaca cctacttcgg ctacagcacc 840
ccctgggggt attttgactt caacagattc cactgccact tctcaccacg tgactggcag 900
cgactcatca acaacaactg gggattccgg ccaaaaagac tcagcttcaa gctcttcaac 960
atccaggtca aggaggtcac gcagaatgaa ggcaccaaga ccgtcgccaa taaccttacc 1020
agcacgattc aggtatttac ggactcggaa taccagctgc cgtacgtcct cggctccgcg 1080
caccagggct gcctgcctcc gttcccggcg gacgtcttca tgattcccca gtacggctac 1140
cttacactga acaatggaag tcaagccgta ggccgttcct ccttctactg cctggaatat 1200
tttccatctc aaatgctgcg aactggaaac aattttgaat tcagctacac cttcgaggac 1260
gtgcctttcc acagcagcta cgcacacagc cagagcttgg accgactgat gaatcctctc 1320
atcgaccagt acctgtacta cttatccaga actcagtcca caggaggaac tcaaggtacc 1380
cagcaattgt tattttctca agctgggcct gcaaacatgt cggctcaggc taagaactgg 1440
ctacctggac cttgctaccg gcagcagcga gtctctacga cactgtcgca aaacaacaac 1500
agcaactttg cttggactgg tgccaccaaa tatcacctga acggaagaga ctctttggta 1560
aatcccggtg tcgccatggc aacccacaag gacgacgagg aacgcttctt cccgtcgagt 1620
ggagtcctga tgtttggaaa acagggtgct ggaagagaca atgtggacta cagcagcgtt 1680
atgctaacca gcgaagaaga aattaaaacc actaaccctg tagccacaga acaatacggt 1740
gtggtggctg acaacttgca gcaaaccaat acagggccta ttgtgggaaa tgtcaacagc 1800
caaggagcct tacctggcat ggtctggcag aaccgagacg tgtacctgca gggtcccatc 1860
tgggccaaga ttcctcacac ggacggcaac ttccaccctt caccgctaat gggaggattt 1920
ggactgaagc acccacctcc tcagatcctg atcaagaaca cgccggtacc tgcggatcct 1980
ccaacaacgt tcagccaggc gaaattggct tccttcatta cgcagtacag caccggacag 2040
gtcagcgtgg aaatcgagtg ggagctgcag aaggagaaca gcaaacgttg gaacccagag 2100
attcagtaca cttcaaacta ctacaaatct acaaatgtgg actttgctgt caatacagag 2160
ggaacttatt ctgagcctcg ccccattggt actcgttacc tcacccgtaa tctgtaa 2217
<210>7
<211>2217
<212>DNA
<213>新AAV血清型,克隆rh.38
<400>7
atggctgctg acggttatct tccagattgg ctcgaggaca acctctctga gggcattcgc 60
gagtggtggg acctgaaacc tggagccccc aagcccaagg ccaaccagca gaagcaggac 120
gacggccggg gtctggtgct tcctggctac aagtacctcg gacccttcaa cggactcgac 180
aagggggagc ccgtcaacgc ggcggacgca gcggccctcg agcacgacaa ggcctacgac 240
cagcagctca aagcgggtga caatccgtac ctgcggtata accacgccga cgccgagttt 300
caggagcgtc tacaagaaga tacgtctttt gggggcaacc tcgggcgagc agtcttccag 360
gccaagaagc gggttctcga acctctcggt ctggttgagg aagctgctaa gacggctcct 420
ggaaagaaga gaccggtaga accgtcacct cagcgttccc ccgactcctc cacgggcatc 480
ggcaagaaag gccagcggcc cgctaaaaag agactgaact ttggtcagac tggcgactca 540
gagtcagtcc ccgaccctca accaatcgga gaaccaccag caggcccctc tggtctggga 600
tctggtacaa tggctgcagg cggtggcgct ccaatggcag acaataacga aggcgccgac 660
ggagtgggta gttcctcagg aaattggcat tgcgattcca catggctggg cgacagagtc 720
atcaccacca gcacccgaac ctgggccctg cccacctaca acaaccacct ctacaagcaa 780
atatccaatg ggacatcggg aggaagcacc aacgacaaca cctacttcgg ctacagcacc 840
ccctgggggt attttgactt caacagattc cactgccact tctcaccacg tgactggcag 900
cgactcatca acaacaactg gggattccgg ccaaaaagac tcagcttcaa gcccttcaac 960
atccaggtca aggaggtcac gcagaatgaa ggcaccaaga ccatcgccaa taaccttacc 1020
agcacgattc aggtatttac ggactcggaa taccagctgc cgtacgtcct cggctccgcg 1080
caccagggct gcctgcctcc gttcccggcg gacgtcttca tgattcccca gtacggctac 1140
cttacactga acaatggaag tcaagccgta ggccgttcct ccttctactg cctggaatat 1200
tttccatctc aaatgctgcg aactggaaac aattttgaat tcagctacac cttcgaggac 1260
gtgcctttcc acagcagcta cgcacacagc cagagcttgg accgactgat gaatcctctc 1320
atcgaccagt acctgcacta cttatccaga actcagtcca caggaggaac tcaaggtacc 1380
cagcaattgt tattttctca agctgggcct gcaaacatgt cggctcaggc taagaactgg 1440
ctacctggac cttgctaccg gcagcagcga gtctctacga cactgtcgca aaacaacaac 1500
agcaactttg cttggactgg tgccaccaaa tatcacctga acggaagaga ctctttggta 1560
aatcccggtg tcgccatggc aacccacaag gacgacgagg aacgcttctt cccgtcgagt 1620
ggagtcctga tgtttggaaa acagggtgct ggaagagaca atgtggacta cagcagcgtt 1680
atgctaacca gcgaagaaga aattaaaacc actaaccctg tagccacaga acaatacggt 1740
gtggtggctg acaacttgca gcaaaccaat acagggccta ttgtgggaaa tgtcaacagc 1800
caaggagcct tacctggcat ggtctggcag aaccgagacg tgtacctgca gggtcccatc 1860
tgggccaaga ttcctcacac ggacggcaac ttccaccctt caccgctaat gggaggattt 1920
ggactgaagc acccacctcc tcagatcctg atcaagaaca cgccggtacc tgcggatcct 1980
ccaacaacgt tcagccaggc gaaattggct tccttcatta cgcagtacag caccggacag 2040
gtcagcgtgg aaatcgagtg ggagctgcag aaggagaaca gcaaacgctg gaacccagag 2100
attcagtaca cttcaaacta ctacaaatct acaaatgtgg actttgctgt caatacagag 2160
ggaacttatt ctgagcctcg ccccattggt actcgctacc tcacccgtaa tctgtaa 2217
<210>8
<211>2217
<212>DNA
<213>新AAV血清型,克隆hu.42
<400>8
atggctgctg acggttatct tccagattgg ctcgaggaca acctctctga gggcattcgc 60
gagtggtggg acctgaaacc tggagccccc aagcccaagg ccaaccagca gaagcaggac 120
gacggccggg gtctggtgct tcctggctac aagtacctcg gacccttcaa cggactcgac 180
aagggggagc ccgtcaacgc ggcggacgca gcggccctcg agcacgacaa ggcctacgac 240
cagcagctca aagcgggtga caatccgtac ctgcggtata accacgccga cgccgagttt 300
caggagcgtc tgcaagaaga tacgtctttt gggggcaacc tcgggcgagc agtcttccag 360
gccaagaagc gggttctcga acctctcggt ctggttgagg aagcggctaa gacggctcct 420
ggaaagaaga gaccggtaga accgtcacct cagcgttccc ccgactcctc cacgggcatc 480
ggcaagaaag gccagcagcc cgctaaaaag agactgaact ttggtcagac tggcgactca 540
gagtcagtcc ccgaccctca accaatcgga gaaccaccag caggcccctc tggtctggga 600
tctggtacaa tggctgcagg cggtggcgct ccaatggcag acaataacga aggcgccgac 660
ggagtgggta gttcctcagg aaattggcat tgcgattcca catggctggg cgacagagtc 720
atcaccacca gcacccgaac ctgggccctg cccacctaca acaaccacct ctacaagcaa 780
atatccaatg ggacatcggg aggaagcacc aacgacaaca cctacttcgg ctacagcacc 840
ccctgggggt attttgactt caacagattc cactgccact tctcaccacg tgactggcag 900
cgactcatca acaacaactg gggattccgg ccaaaaagac tcagcttcaa gctcttcaac 960
atccaggtca aggaggtcac gcagaatgaa ggcaccaaga ccatcgccaa taaccttacc 1020
agcacgattc aggtatttac ggactcggaa taccagctgc cgtacgtcct cggctccgcg 1080
caccagggct gcctgcctcc gttcccggcg gacgtcttca tgattcccca gtacggctac 1140
cttacactga acaatggaag tcaagccgta ggccgttcct ccttctactg cctggaatat 1200
tttccatctc aaatgctgcg aactggaaac aattttgaat tcagctacac cttcgaggac 1260
gtgcctttcc acagcagcta cgcacacagc cagagcttgg accgactgat gaatcctctc 1320
atcgaccagt acctgtacta cttatccaga actcagtcca caggaggaac tcaaggtacc 1380
cagcaattgt tattttctca agctgggcct gcaaacatgt cggctcaggc taagaactgg 1440
ctacctggac cttgctaccg gcagcagcga gtctctacga cactgtcgca aagcaacaac 1500
agcaactttg cttggactgg tgccaccaaa tatcacctga acggaagaga ctctttggta 1560
aatcccggtg tcgccatggc aacccacaag gacgacgagg aacgcttctt cccgtcgagt 1620
ggagtcctga tgtttggaaa acagggtgct ggaagagaca atgtggacta cagcagcgtt 1680
atgctaacca gcgaagaaga aattaaaacc actaaccctg tagccacaga acaatacggt 1740
gtggtggctg acaacttgca gcaaaccaat acagggccta ttgtgggaaa tgtcaacagc 1800
caaggagcct tacctggcat ggtctggcag aaccgagacg tgtacctgca gggtcccatc 1860
tgggccaaga ttcctcacac ggacggcaac ttccaccctt caccgctaat gggaggactt 1920
ggactgaagc acccacctcc tcagatcctg atcaagaaca cgccggtacc tgcggatcct 1980
ccaacaacgt tcagccaggc gaaattggct tccttcatta cgcagtacag caccggacag 2040
gtcagcgtgg aaatcgagtg ggagctgcag aaggagaaca gcaaacgctg gaacccagag 2100
attcagtaca cttcaaacta ctacaaatct acaaatgtgg actttgctgt caatacagag 2160
ggaacttatt ctgagcctcg ccccattggt actcgttacc tcacccgtaa tctgtaa 2217
<210>9
<211>2217
<212>DNA
<213>新AAV血清型,克隆rh.72
<400>9
atggctgctg acggttatct tccagattgg ctcgaggaca acctctctga gggcattcgc 60
gagtggtggg acctgaaacc tggagccccc aagcccaagg ccaaccagca gaagcaggac 120
gacggccggg gtctggtgct tcctggctac aagtacctcg gacccttcaa cggactcgac 180
aagggggagc ccgtcaacgc ggcggacgca gcggccctcg agcacgacaa ggcctacgac 240
cagcagctca aagcgggtga caatccgtac ctgcggtata accacgccga cgccgagttt 300
caggagcgtc tgcaagaaga tacgtctttt gggggcaacc tcgggcgagc agtcttccag 360
gccaagaagc gggttctcga acctctcggt ctggttgagg aagctgctaa gacggctcct 420
ggaaagaaga gaccggtaga accgtcacct cagcgttccc ccgactcctc cacgggcatc 480
ggcaagaaag gccagcagcc cgctaaaaag agactgaact ttggtcagac tggcgactca 540
gagtcagtcc ccgaccctca accaatcgga gaaccaccag caggcccctc tggtctggga 600
tctggtacaa tggctgcagg cggtggcgct ccaatggcag acaataacga aggcgccgac 660
ggagtgggta gttcctcagg aaattggcat tgcgattcca catggctggg cgacagagtc 720
atcaccacca gcacccgaac ctgggccctg cccacctaca acaaccacct ctacaagcaa 780
atatccaatg ggacatcggg aggaagcacc aacgacaaca cctacttcgg ctacagcacc 840
ccctgggggt attttgactt caacagattc cactgccact tctcaccacg tgactggcag 900
cgactcatca acaacaactg gggattccgg ccaaaaagac tcagcttcaa gctcttcaac 960
atccaggtca aggaggtcac gcagaatgaa ggcaccaaga ccatcgccaa taaccttacc 1020
agcacgattc aggtatttac ggactcggaa taccagctgc cgtacgtcct cggctccgcg 1080
caccagggct gcctgcctcc gttcccggcg gacgtcttca tgattcccca gtacggctac 1140
cttacactga acaatggaag tcaagccgta ggccgttcct ccttctactg cctggaatat 1200
tttccatctc aaatgctgcg aactggaaac aattttgaat tcagctacac cttcgaggac 1260
gtgcctttcc acagcagcta cgcacacagc cagagcttgg accgactgat gaatcctctc 1320
atcgaccagt acctgtacta cttatccaga actcagtcca caggaggaac tcaaggtacc 1380
cagcaattgt tattttctca agctgggcct gcaaacatgt cggctcaggc taagaactgg 1440
ctacctggac cttgctaccg gcagcagcga gtctctacga cactgtcgca aaacaacaac 1500
agcaactttg cttggactgg tgccaccaaa tatcacctga acggaagaga ctctttggta 1560
aatcccggtg tcgccatggc aacccacaag gacgacgagg aacgcttctt cccgtcgagt 1620
ggagtcctga tgtttggaaa acagggtgct ggaagagaca atgtggacta cagcagcgtt 1680
atgctaacca gcgaagaaga aattaaaacc actaaccctg tagccacaga acaatacggt 1740
gtggtggctg acaacttgca gcaaaccaat acagggccta ttgtgggaaa tgtcaacagc 1800
caaggagcct tacctggcat ggtctggcag aaccgagacg tgtacctgca gggtcccatc 1860
tgggccaaga ttcctcacac ggacggcaac ttccaccctt caccgctaat gggaggattt 1920
ggactgaagc acccacctcc tcagatcctg atcaagaaca cgccggtacc tgcggatcct 1980
ccaacaacgt tcagccaggc gaaattggct tccttcatta cgcagtacag caccggacag 2040
gtcagcgtgg aaatcgagtg ggagctgcag aaggagaaca gcaaacgctg gaacccagag 2100
attcagtaca cttcaaacta ctacaaatct acaaatgtgg actttgctgt caatacagag 2160
ggaacttatt ctgagcctcg ccccattggt actcgttacc tcacccgtaa tctgtaa 2217
<210>10
<211>2217
<212>DNA
<213>新AAV血清型,克隆hu.37
<400>10
atggctgctg acggttatct tccagattgg ctcgaggaca acctctctga gggcattcgc 60
gagtggtggg acctgaaacc tggagccccc aagcccaagg ccaaccagca gaagcaggac 120
gacggccggg gtctggtgct tcctggctac aagtacctcg gacccttcaa cggactcgac 180
aagggggagc ccgtcaacgc ggcggacgca gcggccctcg agcacgacaa ggcctacgac 240
cagcagctca aagcgggtga caatccgtac ctgcggtata accacgccga cgccgagttt 300
caggagcgtc tgcaagaaga tacgtctttt gggggcaacc tcgggcgagc agtcttccag 360
gccaagaagc gggttctcga acctctcggt ctggttgagg aagctgctaa gacggctcct 420
ggaaagaaga gaccggtaga accgtcacct cagcgttccc ccgactcctc cacgggcatc 480
ggcaagaaag gccagcagcc cgctaaaaag agactgaact ttggtcagac tggcgactca 540
gagtcagtcc ccgaccctca accaatcgga gaaccaccag caggcccctc tggtctggga 600
tctggtacaa tggctgcagg cggtggcgct ccaatggcag acaataacga aggcgccgac 660
ggagtgggta gttcctcagg aaattggcat tgcgattcca catggctggg cgacagagtc 720
atcaccacca gcacccgaac ctgggccctg cccacctaca acaaccacct ctacaagcaa 780
atatccaatg ggacatcggg aggaagcacc aacgacaaca cctacttcgg ctacagcacc 840
ccctgggggt attttgactt caacagattc cactgccact tctcaccacg tgactggcag 900
cgactcatca acaacaactg gggattccgg ccaaaaagac tcagcttcaa gctcttcaac 960
atccaggtca aggaggtcac gcagaatgaa ggcaccaaga ccatcgccaa taaccttacc 1020
agcacgattc aggtatttac ggactcggaa taccagctgc cgtacgtcct cggctccgcg 1080
caccagggct gcctgcctcc gttcccggcg gacgtcttca tgattcccca gtacggctac 1140
cttacactga acaatggaag tcaagccgta ggccgttcct ccttctactg cctggaatat 1200
tttccatctc aaatgctgcg aactggaaac aattttgaat tcagctacac cttcgaggac 1260
gtgcctttcc acagcagcta cgcacacagc cagagcttgg accgactgat gaatcctctc 1320
atcgaccagt acctgtacta cttatccaga actcagtcca caggaggaac tcaaggtacc 1380
cagcaattgt tattttctca agctgggcct gcaaacatgt cggctcaggc taagaactgg 1440
ctacctggac cttgctaccg gcagcagcga gtctctacga cactgtcgca aaacaacaac 1500
agcaactttg cttggactgg tgccaccaaa tatcacctga acggaagaga ctctttggta 1560
aatcccggtg tcgccatggc aacccacaag gacgacgagg aacgcttctt cccgtcgagt 1620
ggagtcctga tgttcggaaa acagggtgct ggaagagaca atgtggacta cagcagcgtt 1680
atgctaacca gcgaagaaga aattaaaacc actaaccccg tagccacaga acaatacggt 1740
gtggtggctg acaacttgca gcaaaccaat acagggccta ttgtgggaaa tgtcaacagc 1800
caaggagcct tacctggcat ggtctggcag aaccgagacg tgtacctgca gggtcccatc 1860
tgggccaaga ttcctcacac ggacggcaac ttccaccctt caccgctaat gggaggattt 1920
ggactgaagc acccacctcc tcagatcctg atcaagaaca cgccggtacc tgcggatcct 1980
ccaacaacgt tcagccaggc gaaattggct tccttcatta cgcagtacag caccggacag 2040
gtcagcgtgg aaatcgagtg ggagctgcag aaggagaaca gcaaacgctg gaacccagag 2100
attcagtaca cttcaaacta ctacaaatct acaaatgtgg actttgctgt caatacagag 2160
ggaacttatt ctgagcctcg ccccattggt actcgttacc tcacccgtaa tctgtaa 2217
<210>11
<211>2217
<212>DNA
<213>新AAV血清型,克隆hu.40
<400>11
atggctgctg acggttatct tccagattgg ctcgaggaca acctctctga gggcattcgc 60
gagtggtggg acctgaaacc tggagccccc aagcccaagg ccaaccagca gaagcaggac 120
gacggccggg gtctggtgct tcctggctac aagtacctcg gacccttcaa cggactcgac 180
aagggggagc ccgtcaacgc ggcggacgca gcggccctcg agcacgacaa ggcctacgac 240
cagcagctca aagcgggtga caatccgtac ctgcggtata accacgccga cgccgagttt 300
caggagcgtc tgcaagaaga tacgtctttt gggggcaacc tcgggcgagc agtcttccag 360
gccaagaagc gggttctcga acctctcggt ctggttgagg aagctgctaa gacggctcct 420
ggaaagaaga gaccggtaga accgtcacct cagcgttccc ccgactcctc cacgggcatc 480
ggcaagaaag gccagcagcc cgctaaaaag agactgagct ttggtcagac tggcgactca 540
gagtcagtcc ccgaccctca accaatcgga gaaccaccag caggcccctc tggtctggga 600
tctggtacaa tggctgcagg cggtggcgct ccaatggcag acaataacga aggcgccgac 660
ggagtgggta gttcctcagg aaattggcat tgcgattcca catggctggg cgacagagtc 720
atcaccacca gcacccgaac ctgggccctg cccacctaca acaaccacct ctacaagcaa 780
atatccaatg ggacatcggg aggaagcacc aacgacaaca cctacttcgg ctacagcacc 840
ccctgggggt attttgactt caacagattc cactgccact tctcaccacg tgactggcag 900
cgactcatca acaacaactg gggattccgg ccaaaaagac tcagcttcaa gctcttcaac 960
atccaggtca aggaggtcac gcagaatgaa ggcaccaaga ccatcgccaa taaccttacc 1020
agcacgattc aggtatttac ggactcggaa taccagctgc cgtacgtcct cggctccgcg 1080
caccagggct gcctgcctcc gttcccggcg gacgtcttca tgattcccca gtacggctac 1140
cttacactga acaatggaag tcaagccgta ggccgttcct ccttctactg cctggaatat 1200
tttccatctc aaatgctgcg aactggaaac aattctgaat tcagctacac cttcgaggac 1260
gtgcctttcc acagcagcta cgcacacagc cagagcttgg accgactgat gaatcctctc 1320
atcgaccagt acctgtacta cttatccaga actcagtcca caggaggaac tcaaggtacc 1380
cagcaattgt tattttctca agctgggcct gcaaacatgt cggctcaggc taagaactgg 1440
ctacctggac cttgctaccg gcagcagcga gtctctacga cactgtcgca aaacaacaac 1500
agcaactttg cttggactgg tgccaccaaa tatcacctga acggaagaga ctctttggta 1560
aatcccggtg tcgccatggc aacccacaag gacgacgagg aacgcttctt cccgtcgagt 1620
ggagtcctga tgtttggaaa acagggtgct ggaagagaca atgtggacta cagcagcgtt 1680
atgctaacca gcgaagaaga aattaaaacc actaaccctg tagccacaga acaatacggt 1740
gtggtggctg acaacttgca gcaaaccaat acagggccta ttgtgggaaa tgtcaacagc 1800
caaggagcct tacctggcat ggtctggcag aaccgagacg tgtacctgca gggtcccatc 1860
tgggccaaga ttcctcacac ggacggcaac ttccaccctt caccgctaat gggaggattt 1920
ggactgaagc acccacctcc tcagatcctg atcaagaaca cgccggtacc tgcggatcct 1980
ccaacaacgt tcagccaggc gaaattggct tccttcatta cgcagtacag caccggacag 2040
gtcagcgtgg aaatcgagtg ggagctgcag aaggagaaca gcaaacgctg gaacccagag 2100
attcagtaca cttcaaacta ctacaaatct acaaatgtgg actttgctgt caatacagag 2160
ggaacttatt ctgagcctcg ccccattggt actcgttacc tcacccgtaa tctgtaa 2217
<210>12
<211>2217
<212>DNA
<213>新AAV血清型,克隆hu.38
<400>12
atggctgctg acggttatct tccagattgg ctcgaggaca acctctctga gggcattcgc 60
gagtggtggg acctgaaacc tggagccccc aagcccaagg ccaaccagca gaagcaggac 120
gacggccggg gtctggtgct tcctggctac aagtacctcg gacccttcaa cggactcgac 180
aagggggagc ccgtcaacgc ggcggacgca gcggccctcg agcacgacaa ggcctacgac 240
cagcagctca aagcgggtga caatccgtac ctgcggtata accacgccga cgccgagttt 300
caggagcgtc tgcaagaaga tacgtctttt gggggcaacc tcgggcgagc agtcttccag 360
gccaagaagc gggttctcga acctctcggt ctggttgagg aagctgctaa gacggctcct 420
ggaaagaaga gaccggtaga accgtcacct cagcgttccc ccgactcctc cacgggcatc 480
ggcaagaaag gccagcagcc cgctaaaaag agactgaact ttggtcagac tggcgactca 540
gagtcagtcc ccgaccctca accaatcgga gaaccacctg caggcccctc tggtctggga 600
tctggtacaa tggctgcagg cggtggcgct ccaatggcag acaataacga aggcgccgac 660
ggagtgggta gttcctcagg aaattggcat tgcgattcca catggctggg cgacagagtc 720
atcaccacca gcacccgaac ctgggccctg cccacctaca acaaccacct ctacaagcaa 780
atatccaatg ggacatcggg agggagcacc aacgacaaca cctacttcgg ctacagcacc 840
ccctgggggt attttgactt caacagattc cactgccact tctcaccacg tgactggcag 900
cgactcatca acaacaactg gggattccgg ccaaaaagac tcagcttcaa gctcttcaac 960
atccaggtca aggaggtcac gcagaatgaa ggcaccaaga ccatcgccaa taaccttacc 1020
agcacgattc aggtatttac ggactcggaa taccagctgc cgtacgtcct cggctccgcg 1080
caccagggct gcctgcctcc gttcccggcg gacgtcttca tgattcccca gtacggctac 1140
cttacactga acaatggaag tcaagccgta ggccgttcct ccttctactg cctggaatat 1200
tttccatctc aaatgctgcg aactggaaac aattttgaat tcagctacac cttcgaggac 1260
gtgcctttcc acagcagcta cgcacacagc cagagcttgg accgactgat gaatcctctc 1320
atcgaccagt acctgtacta cttatccaga actcagtcca caggaggaac tcaaggtacc 1380
cagcaattgt tattttctca agctgggcct gcaaacatgt cggctcaggc taagaactgg 1440
ctacctggac cttgctaccg gcagcagcga gtctctacga cactgtcgca aaacaacaac 1500
agcaactttg cttggactgg tgccaccaaa tatcacctga acggaagaga ctctttggta 1560
aatcccggtg tcgccatggc aacccacaag gacgacgagg aacgcttctt cccgtcgagt 1620
ggagtcctga tgtttggaaa acagggtgct ggaagagaca atgtggacta cagcagcgtt 1680
atgctaacca gcgaagaaga aattaaaacc actaaccctg tagccacaga acaatacggt 1740
gtggtggctg acaacttgca gcaaaccaat acagggccta ttgtgggaaa tgtcaacagc 1800
caaggagcct tacctggcat ggtctggcag aaccgagacg tgtacctgca gggtcccatc 1860
tgggccaaga ttcctcacac ggacggcaac tgccaccctt caccgctaat gggaggattt 1920
ggactgaagc acccacctcc tcagatcctg atcaagaaca cgccggtacc tgcggatcct 1980
ccaacaacgt tcagccaggc gaaattggct tccttcatta cgcagtacag caccggacag 2040
gtcagcgtgg aaatcgagtg ggagctgcag aaggagaaca gcaaacgctg gaacccagag 2100
attcagtaca cttcaaacta ctacaaatct acaaatgtgg actttgctgt caatacagag 2160
ggaacttatt ctgagcctcg ccccattggt actcgttacc tcacccgtaa tctgtaa 2217
<210>13
<211>2217
<212>DNA
<213>新AAV血清型,克隆rh.39
<400>13
atggctgctg acggttatct tccagattgg ctcgaggaca acctctctga gggcattcgc 60
gagtggtggg acctgaaacc tggagccccc aagcccaagg ccaaccagca gaagcaggac 120
gacggccggg gtctggtgct tcctggctac aagtacctcg gacccttcaa cggactcgac 180
aagggggagc ccgtcaacgc ggcggacgca gcggccctcg agcacgacaa ggcctacgac 240
cagcagctca aagcgggtga caatccgtac ctgcggtata accacgccga cgccgagttt 300
caggagcgtc tgcaagaaga tacgtctttt gggggcaacc tcgggcgagc agtcttccag 360
gccaagaagc gggttctcga acctctcggt ctggttgagg aagctgctaa gacggctcct 420
ggaaagaaga gaccggtaga accgtcacct cagcgttccc ccgactcctc cacgggcatc 480
ggcaagaaag gccagcagcc cgctaaaaag agactgaact ttggtcagac tggcgactca 540
gagtcagtcc ccgaccctca accaatcgga gaaccaccag caggcccctc tggtctggga 600
tctggtacaa tggctgcagg cggtggcgct ccaatggcag acaataacga aggcgccgac 660
ggagtgggta gttcctcagg aaattggcat tgcgattcca catggctggg cgacagagtc 720
atcaccacca gcacccgaac ctgggccctg cccacctaca acaaccacct ctacaagcaa 780
atatccaatg ggacatcggg aggaagcacc aacgacaaca cctacttcgg ctacagcacc 840
ccctgggggt attttgactt caacagattc cactgccact tctcaccacg tgactggcag 900
cgactcatca acaacaactg gggattccgg ccaaaaagac tcagcttcaa gctcttcaac 960
atccaggtca aggaggtcac gcagaatgaa ggcaccaaga ccatcgccaa taaccttacc 1020
agcacgattc aggtatttac ggactcggaa taccagctgc cgtacgtcct cggctccgcg 1080
caccagggct gcctgcctcc gttcccggcg gacgtcttca tgattcccca gtacggctac 1140
cttacactga acaatggaag tcaagccgta ggccgttcct ccttctactg cctggaatat 1200
tttccatctc aaatgctgcg aactggaaac aattttgaat tcagctacac cttcgaggac 1260
gtgcctttcc acagcagcta cgcacacagc cagagcttgg accgactgat gaatcctctc 1320
atcgaccagt acctgtacta cttatccaga actcagtcca caggaggaac tcaaggtacc 1380
cagcaattgt tattttctca agctgggcct gcaaacatgt cggctcaggc taagaactgg 1440
ctacctggac cttgctaccg gcagcagcga gtctctacga cactgtcgca aaacaacaac 1500
agcaactttg cttggactgg tgccaccaaa tatcacctga acggaagaga ctctttggta 1560
aatcccggtg tcgccatggc aacccacaag gacgacgagg aacgcttctt cccgtcgagt 1620
ggagtcctga tgtttggaaa acagggtgct ggaagagaca atgtggacta cagcagcgtt 1680
atgctaacca gcgaagaaga aattaaaacc actaaccctg tagccacaga acaatacggt 1740
gtggtggctg ataacttgca gcaaaccaat acggggccta ttgtgggaaa tgtcaacagc 1800
caaggagcct tacctggcat ggtctggcag aaccgagacg tgtacctgca gggtcccatc 1860
tgggccaaga ttcctcacac ggacggcaac ttccaccctt caccgctaat gggaggattt 1920
ggactgaagc acccacctcc tcagatcctg atcaagaaca cgccggtacc tgcggatcct 1980
ccaacaacgt tcagccaggc gaaattggct tccttcatta cgcagtacag caccggacag 2040
gtcagcgtgg aaatcgagtg ggagctgcag aaggagaaca gcaaacgctg gaacccagag 2100
attcagtaca cttcaaacta ctacaaatct acaaatgtgg actttgctgt caatacagag 2160
ggaacttatt ctgagcctcg ccccattggt actcgttacc tcacccgtaa tctgtaa 2217
<210>14
<211>2217
<212>DNA
<213>新AAV血清型,克隆rh.40
<400>14
atggctgctg acggttatct tccagattgg ctcgaggaca acctctctga gggcattcgc 60
gagtggtggg acctgaaacc tggagccccc aagcccaagg ccaaccagca gaagcaggac 120
gacggccggg gtctggtgct tcctggctac aagtacctcg gacccttcaa cggactcgac 180
aagggggagc ccgtcaacgc ggcggacgca gcggccctcg agcacgacaa ggcctacgac 240
cagcagctca aagcgggtga caatccgtac ctgcggtata accacgccga cgccgagttt 300
caggagcgtc tgcaagaaga tacgtctttt gggggcaacc tcgggcgagc agtcttccag 360
gccaagaagc gggttctcga acctctcggt ctggttgagg aagctgctaa gacggctcct 420
ggaaagaaga gaccggtaga accgtcacct cagcgttccc ccgactcctc cacgggcatc 480
ggcaagaaag gccagcagcc cgctaaaaag agactgaact ttggtcagac tggcgactca 540
gagtcagtcc ccgaccctca accaatcgga gaaccaccag caggcccctc tggtctggga 600
tctggtacaa tggctgcagg cggtggcgct ccaatggcag acaataacga aggcgccgac 660
ggagtgggta gttcctcagg aaattggcat tgcgattcca catggctggg cgacagagtc 720
atcaccacca gcacccgaac ctgggccctg cccacctaca acaaccacct ctacaagcaa 780
atatccaatg ggacatcggg aggaagcacc aacgacaaca cctacttcgg ctacagcacc 840
ccctgggggt attttgactt caacagattc cactgccact tctcaccacg tgactggcag 900
cgactcatca acaacaactg gggattccgg ccaaaaagac tcagcttcaa gctcttcaac 960
atccaggtca aggaggtcac gcaggatgaa ggcaccaaga ccatcgccaa taaccttacc 1020
agcacgattc aggtatttac ggactcggaa taccagctgc cgtacgtcct cggctccgcg 1080
caccagggct gcctgcctcc gttcccggcg gacgtcttca tgattcccca gtacggctac 1140
cttacactga acaatggaag tcaagccgta ggccgttcct ccttctactg cctggaatat 1200
tttccatctc aaatgctgcg aactggaaac aattttgaat tcagctacac cttcgaggac 1260
gtgcctttcc acagcagcta cgcacacagc cagagcttgg accgactgat gaatcctctc 1320
atcgaccagt acctgtacta cttatccaga actcagtcca caggaggaac tcaaggtacc 1380
cagcaattgt tattttctca agctgggcct gcaaacatgt cggctcgggc taagaactgg 1440
ctacctggac cttgctaccg gcagcagcga gtctctacga cactgtcgca aaacaacaac 1500
agcaactttg cttggactgg tgccaccaaa tatcacctga acggaagaga ctctttggta 1560
aatcccggtg ttgctatggc aacgcataag gacgacgagg aacgtttctt tccatcgagc 1620
ggagtcctga tgtttggaaa acagggtgct ggaagagaca atgtggacta tagcagcgtt 1680
atgctaacca gcgaggaaga aattaaaacc actaaccctg tagccacaga acaatacggt 1740
gtggtggctg acaacttgca gcaagccaat acagggccta ttgtgggaaa tgtcaacagc 1800
caaggagcct tacctggcat ggtctggcag aaccgagacg tgtacctgca gggtcccatc 1860
tgggccaaaa ttcctcacac ggacggcaat tttcacccgt ctcctctgat gggcggcttt 1920
ggactgaagc acccacctcc ccagatcctg atcaagaata cgccggtacc tgcggatcct 1980
ccaacgacgt tcagccaggc aaaattggct tccttcatca cgcagtacag caccggccag 2040
gtcagcgtgg agatcgagtg ggagctgcag aaggagaaca gcaagcgatg gaacccagaa 2100
attcagtaca cttccaacta ctacaaatct acaaatgtgg actttgctgt caattctgag 2160
ggtacatatt cagagcctcg ccccattggt actcgttatc tgacacgtaa tctgtaa 2217
<210>15
<211>2217
<212>DNA
<213>新AAV血清型,克隆rh.64
<400>15
atggctgccg atggttatct tccagattgg ctcgaggaca acctctctga gggcattcgc 60
gagtggtggg acctgaaacc tggagccccg aaacccaaag ccaaccagca aaagcaggac 120
gacggccggg gtctggtgct tcctggctac aagtacctcg gacccttcaa cggactcgac 180
aagggggagc ccgtcaacgc ggcggacgca gcggccctcg agcacgacaa ggcctacgac 240
cagcagctca aagcgggtga caatccgtac ctgcggtata atcacgccga cgccgagttt 300
caggagcgtc tgcaagaaga tacgtctttt gggggcaacc tcgggcgagc agtcttccag 360
gccaagaagc gggttctcga acctctcggt ctggttgagg aaggcgctaa gacggctcct 420
ggaaagaaga gaccggtaga gccgtcacca cagcgttccc ccgactcctc cacgggcatc 480
ggcaagaaag gccagcagcc cgccagaaag agactcaatt tcggtcagac tggcgactca 540
gagtcagtcc ccgaccctca acctatcgga gaacctccag cagcgccctc tagtgtggga 600
tctggtacaa tggctgcagg cggtggcgca ccaatggcag acaataacga aggtgccgac 660
ggagtgggta gttcctcggg aaattggcat tgcgattcca catggctggg cgacagagtc 720
atcaccacca gcacccgaac ctgggccctg cccacctaca acaaccacct ctacaagcaa 780
atctccaacg ggacctcggg aggcagcacc aacgacaaca cctactttgg ctacagcacc 840
ccctgggggt attttgactt taacagattc cactgccact tctcaccacg tgactggcag 900
cgactcatca acaacaactg gggattccgg cccaagagac tcagcttcaa gctcttcaac 960
atccaggtca aagaggtcac gcagaatgaa ggcaccaaga ccatcgccaa taacctcacc 1020
agcaccatcc aggtgtttac ggactcggaa taccagctgc cgtacgtcct cggctctgcc 1080
caccagggct gcctgcctcc gttcccggcg gacgtcttca tgattcctca gtacggctac 1140
ctgactctca acaacggtag tcaggccgtg ggacgttcct ccttctactg cctggagtac 1200
ttcccctctc agatgctgag aacgggcaac aacttttcct tcagctacac tttcgaggac 1260
gtgcctttcc acagcagcta cgcgcacagc cagagtttgg acaggctgat gaatcctctc 1320
atcgaccagt acctgtacta cctgtcaaga acccagtcta cgggaggcac agcgggaacc 1380
cagcagttgc tgttttctca ggccgggcct agcaacatgt cggctcaggc cagaaactgg 1440
ctgcctggac cctgctacag acagcagcgc gtctccacga cactgtcgca aaacaacaac 1500
agcaactttg cctggactgg tgccaccaag tatcatctga acggcagaga ctctctggtg 1560
aatccgggcg tcgccatggc aaccaacaag gacgacgagg accgcttctt cccatccagc 1620
ggcatcctca tgtttggcaa gcagggagct ggaaaagaca acgtggacta tagcaacgtg 1680
atgctaacca gcgaggaaga aatcaagacc accaaccccg tggccacaga acagtatggc 1740
gtggtggctg ataacctaca gcagcaaaac accgctccta ttgtgggggc cgtcaacagc 1800
cagggagcct tacctggcat ggtctggcag aaccgggacg tgtacctgca gggtcctatt 1860
tgggccaaga ttcctcacac agatggcaac tttcacccgt ctcctttaat gggcggcttt 1920
ggacttaaac atccgcctcc tcagatcctc atcaaaaaca ctcctgttcc tgcggatcct 1980
ccaacagcgt tcaaccaggc caagctgaat tctttcatca cgcagtacag caccggacaa 2040
gtcagcgtgg agatcgtgtg ggagctgcag aaggagaaca gcaagcgcag gaacccagag 2100
attcagtata cttccaacta ctacaaatct acaaatgtgg actttgctgt taatactgag 2160
ggtgtttact ctgagcctcg ccccattggc actcgttacc tcacccgtaa tctgtaa 2217
<210>16
<211>2217
<212>DNA
<213>新AAV血清型,克隆rh.68
<400>16
atggctgccg atggttatct tccagattgg ctcgaggaca acctctctga gggcattcgc 60
gagtggtggg acctgaaacc tggagccccg aaacccaaag ccaaccagca aaagcaggac 120
gacggccggg gtctggtgct tcctggctac aagtacctcg gacccttcaa cggactcgac 180
aagggggagc ccgtcaacgc ggcggacgca gcggccctcg agcacgacaa ggcctacgac 240
cagcagctca aagcgggtga caatccgtac ctgcggtata atcacgccga cgccgagttt 300
caggagcgtc tgcaagaaga tacgtctttt gggggcaacc tcgggcgagc agtcttccag 360
gccaagaagc gggttctcga acctctcggt ctggttgagg aaggcgctaa gacggctcct 420
ggaaagaaga gaccggtaga gccgtcacca cagcgttccc ccgactcctc cacgggcatc 480
ggcaagaaag gccagcagcc cgccagaaag agactcaatt tcggtcagac tggcgactca 540
gagtcagtcc ccgaccctca acctatcgga gaacctccag cagcgccctc tagtgtggga 600
tctggtacaa tggctgcagg cggtggcgca ccaatggcag acaataacga aggtgccgac 660
ggagtgggta gttcctcggg aaattggcat tgcgattcca catggctggg cgacagagtc 720
atcaccacca gcacccgaac ctgggccctg cccacctaca acaaccacct ctacaagcaa 780
atctccaacg ggacctcggg aggcagcacc aacgacaaca cctactttgg ctacagcacc 840
ccctgggggt attttgactt taacagattc cactgccact tctcaccacg tgactggcag 900
cgactcatca acaacaactg gggattccgg cccaagagac tcagcttcaa gctcttcaac 960
atccaggtca aagaggtcac gcagaatgaa ggcaccaaga ccatcgccaa taacctcacc 1020
agcaccatcc aggtgtttac ggactcggaa taccagctgc cgtacgtcct cggctctgcc 1080
caccagggct gcctgcctcc gttcccggcg gacgtcttca tgattcctca gtacggctac 1140
ctgactctca acaacggtag tcaggccgtg ggacgttcct ccttctactg cctggagtac 1200
ttcccctctc agatgctgag aacgggcaac aacttttcct tcagctacac tttcgaggac 1260
gtgcctttcc acagcagcta cgcgcacagc cagagtttgg acaggctgat gaatcctctc 1320
atcgaccagt acctgtacta cctgtcaaga acccagtcta cgggaggcac agcgggaacc 1380
cagcagttgc tgttttctca ggccgggcct agcaacatgt cggctcaggc cagaaactgg 1440
ctgcctggac cctgctacag acagcagcgc gtctccacga cactgtcgca aaacaacaac 1500
agcaactttg cctggactgg tgccaccaag tatcatctga acggcagaga ctctctggtg 1560
aatccgggcg tcgccatggc aaccaacaag gacgacgagg accgcttctt cccacccagc 1620
ggcatcctca tgtttggcaa gcagggagct ggaaaagaca acgtggacta tagcaacgtg 1680
atgctaacca gcgaggaaga aatcaagacc accaaccccg tggccacaga acagtatggc 1740
gtggtggctg ataacctaca gcagcaaaac accgctccta ttgtgggggc cgtcaacagc 1800
cagggagcct tacctggcat ggtctggcag aaccgggacg tgtacctgca gggtcctatt 1860
tgggccaaga ttcctcacac agatggcaac tttcacccgt ctcctttaat gggcggcttt 1920
ggacttaaac atccgcctcc tcagatcctc atcaaaaaca ctcctgttcc tgcggatcct 1980
ccaacagcgt tcaaccaggc caagctgaat tctttcatca cgcagtacag caccggacaa 2040
gtcagcgtgg tgatcgagtg ggagctgcag aaggagaaca gcaagcgctg gaacccagag 2100
attcagtata cttccaacta ctacaaatct acaaatgtgg actttgctgt taatactgag 2160
ggtgtttact ctgagcttcg ccccattggc actcgttacc tcacccgtaa tctgtaa 2217
<210>17
<211>2217
<212>DNA
<213>新AAV血清型,克隆rh.53
<400>17
atggctgccg atggttatct tccagattgg ctcgaggaca acctctctga gggcattcgc 60
gagtggtggg acctgaaacc tggagccccg aaacccaaag ccaaccagca aaagcaggac 120
gacggccggg gtctggtgct tcctggctac aagtacctcg gacccttcaa cggactcgac 180
aagggggagc ccgtcaacgc ggcggacgca gcggccctcg agcacgacaa ggcctacgac 240
cagcagctca aagcgggtga caatccgtac ctgcggtata atcacgccga cgccgagttt 300
caggagcgtc tgcaagaaga tacgtctttt gggggcaacc tcgggcgagc agtcttccag 360
gccaagaagc gggttctcga acctctcggt ctggttgagg aaggcgctaa gacggctcct 420
ggaaagaaga gaccggtaga gccgtcacca cagcgttccc ccgactcctc cacgggcatc 480
ggcaagaaag gccagcagcc cgccagaaag agactcaatt tcggtcagac tggcgactca 540
gagtcagtcc ccgaccctca acctatcgga gaacctccag cagcgccctc tagtgtggga 600
tctggtacaa tggctgcagg cggtggcgca ccaatggcag acaataacga aggtgccgac 660
ggagtgggta gttcctcggg aaattggcat tgcgattcca catggctggg cgacagagtc 720
atcaccacca gcacccgaac ctgggccctg cccacctaca acaaccacct ctacaagcaa 780
atctccaacg ggacctcggg aggcagcacc aacgacaaca cctactttgg ctacagcacc 840
ccctgggggt attttgactt taacagattc cactgccact tctcaccacg tgactggcag 900
cgactcatca acaacaactg gggattccgg cccaagagac tcagcttcaa gctcttcaac 960
atccaggtca aagaggtcac gcagaatgaa ggcaccaaga ccatcgccaa taacctcacc 1020
agcaccatcc aggtgtttac ggactcggaa taccagctgc cgtacgtcct cggctctgcc 1080
caccagggct gcctgcctcc gttcccggcg gacgtcttca tgattcctca gtacggctac 1140
ctgactctca acaacggtag tcaggccgtg ggacgttcct ccttctactg cctggagtac 1200
ttcccctctc agatgctgag aacgggcaac aacttttcct tcagctacac tttcgaggac 1260
gtgcctttcc acagcagcta cgtgcacagc cagagtttgg acaggctgat gaatcctctc 1320
atcgaccagt acctgtacta cctgtcaaga acccagtcta cgggaggcac agcgggaacc 1380
cagcagttgc tgttttctca ggccgggcct agcaacatgt cggctcaggc cagaaactgg 1440
ctgcctggac cctgctacag acagcagcgc gtctccacga cactgtcgca aaacaacaac 1500
agcaactttg cctggactgg tgccaccaag tatcatctga acggcagaga ctctctggtg 1560
aattcgggcg tcgccatggc aaccaacaag gacgacgagg accgcttctt cccatccagc 1620
ggcatcctca tgtttggcaa gcagggagct ggaaaagaca acgtggacta tagcaacgtg 1680
atgctaacca gcgaggaaga aatcaagacc accaaccccg tggccacaga acagtatggc 1740
gtggtggctg ataacctaca gcagcaaaac accgctccta ttgtgggggc cgtcaacagc 1800
cagggagcct tacctggcat ggtctggcag aaccgggacg tgtacctgca gggtcctatt 1860
tgggccaaga ttcctcacac agatggcaac tttcacccgt ctcctttaat gggcggcttt 1920
ggacttaaac atccgcctcc tcagatcctc atcaaaaaca ctcctgttcc tgcggatcct 1980
ccaacagcgt tcaaccaggc caagctgaat tctttcatca cgcagtacag caccggacaa 2040
gtcagcgtgg agatcgagtg ggagctgcag aaggagaaca gcaagcgctg gaacccagag 2100
attcagtata cttccaacta ctacaaatct acaaatgtgg actttgctgt taatactgag 2160
ggtgtttact ctgagcctcg ccccattggc actcgttacc ccacccgtaa tctgtaa 2217
<210>18
<211>2217
<212>DNA
<213>新AAV血清型,克隆rh.52
<400>18
atggctgccg atggttatct tccagattgg ctcgaggaca acctctctga gggcattcgc 60
gagtggtggg acctgaaacc tggagccccg aaacccaaag ccaaccagca aaagcaggac 120
gacggccggg gtctggtgct tcctggctac aagtacctcg gacccttcaa cggactcgac 180
aagggggagc ccgtcaacgc ggcggacgca gcggccctcg agcacgacaa ggcctacgac 240
cagcagctca aagcgggtga caatccgtac ctgcggtata atcacgccga cgccgagttt 300
caggagcgtc tgcaagaaga tacgtctttt gggggcaacc tcgggcgagc agtcttccag 360
gccaagaagc gggttctcga acctctcggt ctggttgagg aaggcgctaa gacggctcct 420
ggaaagaaga gaccggtaga gccgtcacca cagcgttccc ccgactcctc cacgggcatc 480
ggcaagaaag gccagcagcc cgccagaaag agactcaatt tcggtcagac tggcgactca 540
gagtcagtcc ccgaccctca acctatcgga gaacctccag cagcgccctc tagtgtggga 600
tctggtacaa tggctgcagg cggtggcgca ccaatggcag acaataacga aggtgccgac 660
ggagtgggta gttcctcggg aaattggcat tgcgattcca catggctggg cgacagagtc 720
atcaccacca gcacccgaac ctgggccctg cccacctaca acaaccacct ctacaagcaa 780
atctccaacg ggacctcggg aggcagcacc aacgacaaca cctactttgg ctacagcacc 840
ccctgggggt attttgactt taacagattc cactgccact tctcaccacg tgactggcag 900
cgactcatca acaacaactg gggattccgg cccaagagac tcagcttcaa gctcttcaac 960
atccaggtca aagaggtcac gcagaatgaa ggcaccaaga ccatcgccaa tagcctcacc 1020
agcaccatcc aggtgtttac ggactcggaa taccagctgc cgtacgtcct cggctctgcc 1080
caccagggct gcctgcctcc gttcccggcg gacgtcttca tgattcctca gtacggctac 1140
ctgactccca acaacggtag tcaggccgtg ggacgttcct ccttctactg cctggagtac 1200
ttcccctctc agatgctgag aacgggcaac aacttttcct tcagctacac tttcgaggac 1260
gtgcctttcc acagcagcta cgcgcacagc cagagtttgg acaggctgat gaatcctctc 1320
atcgaccagt acctgtacta cctgtcaaga acccagtcta cgggaggcac agcgggaacc 1380
cagcagttgc tgtcttctca ggccgggcct agcaacatgt cggctcaggc cagaaactgg 1440
ctgcctggac cctgctacag acagcagcgc gtctccacga cactgtcgca aaacaacaac 1500
agcaactttg cctggactgg tgccaccaag tatcatctga acggcagaga ctctctggtg 1560
aatccgggcg tcgccatggc aaccaacaag gacgacgagg accgcttctt cccatccagc 1620
ggcatcctca tgtttggcaa gcagggagct ggaaaagaca acgtggacta tagcaacgtg 1680
atgctaacca gcgaggaaga aatcaagacc accaaccccg tggccacaga acagtatggc 1740
gtggtggctg ataacctaca gcagcaaaac accgctccta ttgtgggggc cgtcaacagc 1800
cagggagcct tacctggcat ggtctggcag aaccgggacg tgtacttgca gggtcctatt 1860
tgggccaaga ttcctcacac agatggcaac tttcacccgt ctcctttaat gggcggcttt 1920
ggacttaaac atccgcctcc tcagatcctc atcaaaaaca ctcctgttcc tgcggatcct 1980
ccaacagcgt tcaaccaggc caagctgaat tctttcatca cgcagtacag caccggacaa 2040
gtcagcgtgg agatcgagtg ggagctgcag aaggagaaca gcaagcgctg gaacccagag 2100
attcagtata cttccaacta ctacaaatct acaaatgtgg actttgctgt taatactgag 2160
ggtgtttact ctgagcctcg ccccattggc actcgttacc tcacccgtaa tctgtaa 2217
<210>19
<211>2217
<212>DNA
<213>新AAV血清型,克隆rh.46
<400>19
atggctgccg atggttatct tccagattgg ctcgaggaca acctctctga gggcattcgc 60
gagtggtggg acctgaaacc tggagccccg aaacccaaag ccaaccagca aaagcaggac 120
gacggccggg gtctggtgct tcctggctac aagtacctcg gacccttcaa cggactcgac 180
aagggggagc ccgtcaacgc ggcggacgca gcggccctcg agcacgacaa ggcctacgac 240
cagcagctca aagcgggtga caatccgtac ctgcggtata atcacgccga cgccgagttt 300
caggagcgtc tgcaagaaga tacgtctttt gggggcaacc tcgggcgagc agtcttccag 360
gccaagaagc gggttctcga acctctcggt ctggttgagg aaggcgctaa gacggctcct 420
ggaaagaaga gaccggtaga gccgtcacca cagcgttccc ccgactcctc cacgggcatc 480
ggcaagaaag gccagcagcc cgccagaaag agactcaatt tcggtcagac tggcgactca 540
gagtcagtcc ccgaccctca acctatcgga gaacctccag cagcgccctc tagtgtggga 600
tctggtacaa tggctgcagg cggtggcgca ccaatggcag acaataacga aggtgccgac 660
ggagtgggta gttcctcggg aaattggcat tgcgattcca catggctggg cgacagagtc 720
atcaccacca gcacccgaac ctgggccctg cccacctaca acaaccacct ctacaagcaa 780
atctccaacg ggacctcggg aggcagcacc aacgacaaca cctactttgg ctacagcacc 840
ccctgggggt attttgactt taacagattc cactgccact tctcaccacg tgactggcag 900
cgactcatca acaacaactg gggattccgg cccaagagac tcagcttcaa gctcttcaac 960
atccaggtca aagaggtcac gcagaatgaa ggcaccaaga ccatcgccaa taacctcacc 1020
agcaccatcc aggtgtttac ggactcggaa taccagctgc cgtacgtcct cggctctgcc 1080
caccagggct gcctgcctcc gttcccggcg gacgtcttca tgattcctca gtacggctac 1140
ctgactctca acaacggtag tcaggccgtg ggacgttcct ccttctactg cctggagtac 1200
ttcccctctc agatgctgag aacgggcaac aacttttcct tcagctacac tttcgaggac 1260
gtgcctttcc acagcagcta cgcgcacagc cagagtttgg acaggctgat gaatcctctc 1320
atcgaccagt acctgtacta cctgtcaaga acccagtcta cgggaggcac agcgggaacc 1380
cagcagttgc tgttttctca ggccgggcct agcaacatgt cggctcaggc cagaaactgg 1440
ctgcctggac cctgctacag acagcagcgc gtctccacga cactgtcgca aaacaacaac 1500
agcaactttg cctggactgg tgccaccaag tatcatctga acggcagaga ctctctggtg 1560
aatccgggcg tcgccatggc aaccaacaag gacgacgagg accgcttctt cccatccagc 1620
ggcatcctca tgtttggcaa gcagggagct ggaaaagaca acgtggacta tagcaacgtg 1680
atgctaacca gcgaggaaga aatcaaggcc accaaccccg tggccacaga acagtatggc 1740
gtggtggctg ataacctaca gcagcaaaac accgctccta ttgtgggggc cgtcaacagc 1800
cagggagcct tacctggcat ggtctggcag aaccgggacg tgtacctgca gggtcctatt 1860
tgggccaaga ttcctcacac agatggcaac tttcacccgt ctcctttaat gggcggcttt 1920
ggacttaaac atccgcctcc tcagatcctc atcaaaaaca ctcctgttcc tgcggatcct 1980
ccaacagcgt tcaaccaggc caagctgaat tctttcatca cgcagtacag caccggacaa 2040
gtcagcgtgg agatcgagtg ggagctgcag aaggagaaca gcaagcgctg gaacccagag 2100
attcagtata cttccaacta ctacaaatct acaaatgtgg actttgctgt taatactgag 2160
ggtgtttact ctgagcctcg ccccattggc actcgttacc tcacccgtaa tctgtaa 2217
<210>20
<211>2217
<212>DNA
<213>新AAV血清型,克隆rh.70
<400>20
atggctgccg atggttatct tccagattgg ctcgaggaca acctctctga gggcattcgc 60
gagtggtggg acctgaaacc tggagccccg aaacccaaag ccaaccagca aaagcaggac 120
gacggccggg gtctggtgct tcctggctac aagtacctcg gacccttcaa cggactcgac 180
aagggggagc ccgtcaacgc ggcggacgca gcggccctcg agcacgacaa ggcctacgac 240
cagcagctca aagcgggtga caatccgtac ctgcggtata atcacgccga cgccgagttt 300
caggagcgtc tgcaagaaga tacgtctttt gggggcaacc tcgggcgagc agtcttccag 360
gccaagaagc gggttctcga acctctcggt ctggttgagg aaggcgctaa gacggctcct 420
ggaaagaaga gaccggtaga gccgtcacca cagcgttccc ccgactcctc cacgggcatc 480
ggcaagaaag gccagcagcc cgccagaaag agactcaatt tcggtcagac tggcgactca 540
gagtcagtcc ccgaccctca acctatcgga gaacctccag cagcgccctc tagtgtggga 600
tctggtacaa tggctgcagg cggtggcgca ccaatggcag acaataacga aggtgccgac 660
ggagtgggta gttcctcggg aaattggcat tgcgattcca catggctggg cgacagagtc 720
atcaccacca gcacccgaac ctgggccctg cccgcctaca acaaccacct ctacaagcaa 780
atctccaacg ggacctcggg aggcagcacc aacgacaaca cctactttgg ctacagcacc 840
ccctgggggt attttgactt taacagattc cactgccact tctcaccacg tgactggcag 900
cgactcatca acaacaactg gggattccgg cccaagagac tcagcttcaa gctcttcaac 960
atccaggtca aagaggtcac gcagaatgaa ggcaccaaga ccatcgccaa taacctcacc 1020
agcaccatcc aggtgtttac ggactcggaa taccagctgc cgtacgtcct cggctctgcc 1080
caccagggct gcctgcctcc gttcccggcg gatgtcttca tgattcctca gtacggctac 1140
ctgactctca acaacggtag tcaggccgtg ggacgttcct ccttctactg cctggagtac 1200
ttcccctctc agatgctgag aacgggcaac aacttttcct tcagctacac tttcgaggac 1260
gtgcctttcc acagcagcta cgcgcacagc cagagtttgg acaggctgat gaatcctctc 1320
atcgaccagt acctgtacta cctgtcaaga acccagtcta cgggaggcac agcgggaacc 1380
cagcagttgc tgttttctca ggccgggcct agcaacatgt cggctcaggc cagaaactgg 1440
ctgcctggac cctgctacag acagcagcgc gtctccacga cactgtcgca aaacaacaac 1500
agcaactttg cctggactgg tgccaccaag tatcatctga gcggcagaga ctctctggtg 1560
aatccgggcg tcgccatggc aaccaacaag gacgacgagg accgcttctt cccatccagc 1620
ggcatcctca tgtttggcaa gcagggagct ggaaaagaca acgtggacta tagcaacgtg 1680
atgctaacca gcgaggaaga aatcaagacc accaaccccg tggccacaga acagtatggc 1740
gtggtggctg ataacctaca gcagcaaaac accgctccta ttgtgggggc cgtcaacagc 1800
cagggagcct tacctggcat ggtctggcag aaccgggacg tgtacctgca gggtcctatt 1860
tgggccaaga ttcctcacac agatggcaac tttcacccgt ctcctttaat gggcggcttt 1920
ggacttaaac atccgcctcc tcagatcctc atcaaaaaca ctcctgttcc tgcggatcct 1980
ccaacagcgt tcaaccaggc caagctgaat tcttccatca cgcagtacag caccggacaa 2040
gtcagcgtgg agatcgagtg ggagctgcag aaggagaaca gcaagcgctg gaacccagag 2100
attcagtata cttccaacta ctacaaatct acaaatgtgg actttgctgt taatactgag 2160
ggtgtttact ctgagcctcg ccccattggc actcgttacc tcacccgtaa tttgtaa 2217
<210>21
<211>2217
<212>DNA
<213>新AAV血清型,克隆rh.61
<400>21
atggctgccg atggttatct tccagattgg ctcgaggaca acctctctga gggcattcgc 60
gagtggtggg acctgaaacc tggagccccg aaacccaagg ccaaccagca aaagcaggac 120
gacggccggg gtctggtgct tcctggctac aagtacctcg gacccttcaa cggactcgac 180
aagggggagc ccgtcaacgc ggcggacgca gcggccctcg agcacgacaa ggcctacgac 240
cagcagctca aagcgggtga caatccgtac ctgcggtata atcacgccga cgccgagttt 300
caggagcgtc tgcaagaaga tacgtctttt gggggcaacc tcgggcgagc agtcttccag 360
gccaagaagc gggttctcga acctctcggt ctggttgagg aaggcgctaa gacggctcct 420
ggaaagaaga gaccggtaga gccgtcacca cagcgttccc ccgactcctc cacgggcatc 480
ggcaagaaag gccagcagcc cgccagaaag agactcaatt tcggtcagac tggcgactca 540
gagtcagtcc ccgaccctca acctatcgga gaacctccag cagcgccctc tagtgtggga 600
tctggtacaa tggctgcagg cggtggcgca ccaatggcag acaataacga aggtgccgac 660
ggagtgggta gttcctcggg aaattggcat tgcgattcca catggctggg cgacagagtc 720
atcaccacca gcacccgaac ctgggccctg cccacctaca acaaccacct ctacaagcaa 780
atctccaacg ggacctcggg aggcagcacc aacgacaaca cctactttgg ctacagcacc 840
ccctgggggt attttgactt taacagattc cactgccact tctcaccacg tgactggcag 900
cgacccatca acaacaactg gggattccgg cccaagagac tcagcttcaa gctcttcaac 960
atccaggtca aagaggtcac gcagaatgaa ggcaccaaga ccatcgccaa taacctcacc 1020
agcaccatcc aggtgtttac ggactcggaa taccagctgc cgtacgtcct cggctctgcc 1080
caccagggct gcctgcctcc gttcccggcg gacgtcttca tgattcctca gtacggctac 1140
ctgactctca acaacggtag tcaggccgtg ggacgttcct ccttctactg cctggagtac 1200
ttcccctctc agatgctgag aacgggcaac aacttttcct tcagctaccc tttcgaggac 1260
gtgcctttcc acagcagcta cgcgcacagc cagagtttgg acaggctgat gaatcctctc 1320
atcgaccagt acctgtacta cctgtcaaga acccagtcta cgggaggcac agcgggaacc 1380
cagcagttgc tgttttctca ggccgggcct agcaacatgt cggctcaggc cagaaactgg 1440
ctgcctggac cctgctacag acagcagcgc gtctccacga cactgtcgca aaacaacaac 1500
agcaactttg cctggactgg tgccaccaag tatcatctga acggcagaga ctctctggtg 1560
aatccgggcg tcgccatggc aaccaacaag gacgacgagg accgcttctt cccatccagc 1620
ggcatcctca tgtttggcaa gcagggagct ggaaaagaca acgtggacta tagcaacgtg 1680
atgctaacca gcgaggaaga aatcaagacc accaaccccg tggccacaga acagtatggc 1740
gtggtggctg ataacctaca gcagcaagac accgctccta ttgtgggggc cgtcaacagc 1800
cagggagcct tacctggcat ggtctggcag aaccgggacg tgtacctgca gggtcctatt 1860
tgggccaaga ttcctcacac agatggcaac tttcacccgt ctcctttaat gggcggcttt 1920
ggacttaaac atccgcctcc tcaggtcctc atcaaaaaca ctcctgttcc tgcggatcct 1980
ccaacagcgt tcaaccaggc caagctgaat tctttcatca cgcagtacag caccggacaa 2040
gtcagcgtgg agatcgagtg ggagctgcag aaggagaaca gcaagcgctg gaacccagag 2100
attcagtata cttccaacta ctacaaatct acaaatgtgg actttgctgt taatactgag 2160
ggtgtttact ctgagcctcg ccccattggc actcgttacc tcacccgtaa tctgtaa 2217
<210>22
<211>2217
<212>DNA
<213>新AAV血清型,克隆rh.51
<400>22
atggttgccg atggttatct tccagattgg ctcgaggaca acctctctga gggcattcgc 60
gagtggtggg acctgaaacc tggagccccg aaacccaaag ccaaccagca aaagcagggc 120
gacggccggg gtctggtgct tcctggctac aagtacctcg gacccttcaa cggactcgac 180
aagggggagc ccgtcaacgc ggcggacgca gcggccctcg agcacgacaa ggcctacgac 240
cagcagctta aagcgggtga caatccgtac ctgcggtata atcacgccga cgccgagctt 300
caggagcgtc tgcaagaaga tacgtctttt gggggcaacc tcgggcgagc agtcttccag 360
gccaagaagc gggttctcga acctctcggt ctggttgagg aaggcgctaa gacggctcct 420
ggaaagaaga gaccggtaga gccgtcacca cagcgttccc ccgactcctc cacgggcatc 480
ggcaagaaag gccagcagcc cgccagaaag agactcaatt tcggtcagac tggcgactca 540
gagtcagtcc ccgaccctca acctatcgga gaacctccag cagcgccctc tagtgtggga 600
tctggtacaa tggctgcagg cggtggcgcg ccaatggcag acaataacga aggtgccgac 660
ggagtgggta gttcctcggg aaattggcat tgcgattcca catggctggg cgacagagtc 720
atcaccacca gcacccgaac ctgggccctg cccacctaca acaaccacct ctacaagcaa 780
atctccaacg ggacctcggg aggcagcacc aacgacaaca cctactttgg ctacagcacc 840
ccctgggggt attttgactt taacagattc cactgccact tctcaccacg tgactggcag 900
cgactcatca acaacaactg gggattccgg cccaagagac tcagcttcaa gctcttcaac 960
atccaggtca aagaggtcac gcagaatgaa ggcaccaaga ccatcgccaa taacctcacc 1020
agcaccatcc aggtgtttac ggactcggaa taccagctgc cgtacgtcct cggctctgcc 1080
caccagggct gccagcctcc gttcccggcg gacgtcttca tgattcctca gtacggctac 1140
ctgactctca acaacggtag tcaggccgtg ggacgttcct ccttctactg cctggagtac 1200
ttcccctctc agatgctgag aacgggcaac aacttttcct tcagctacac tttcgaggac 1260
gtgcctttcc acagcagcta cgcgcacagc cagagtttgg acaggctgat gaatcctctc 1320
atcgaccagt acctgtacta cctgtcaaga acccagtcta cgggaggcac agcgggaacc 1380
cagcagttgc tgttttctca ggccgggcct agcaacatgt cggctcaggc cagaaactgg 1440
ctgcctggac cctgctacag acagcagcgc gtctccacga cactgtcgca aaacaacaac 1500
agcaactttg cctggactgg tgccaccaag tatcatctga acggcagaga ctctctggtg 1560
aatccgggcg tcgccatggc aaccaacaag gacgacgagg accgcttctt cccatccagc 1620
ggcatcctca tgtttggcaa gcagggagct ggaaaagaca acgtggacta tagcaacgtg 1680
atgctaacca gcgaggaaga aatcaagacc accaaccccg tggccacaga acagtatggc 1740
gtggtggctg ataacctaca gcagcaaaac accgctccta ttgtgggggc cgtcaacagc 1800
cagggagcct tacctggcat ggtctggcag aaccgggacg tgtacctgca gggtcctatt 1860
tgggccaaga ttcctcacac agatggcaac tttcacccgt ctcctttaat gggcggcttt 1920
ggacttaaac atccgcctcc tcagatcctc atcaaaaaca ctcctgttcc tgcggatcct 1980
ccaacagcgt tcaaccaggc caagctgaat tctttcatca cgcagtacag caccggacaa 2040
gtcagcgtgg agatcgagtg ggagccgcag aaggagaaca gcaagcgctg gaacccagag 2100
attcagtata cttccaacta ctacaaatct acaaatgtgg actttgctgt taatactgag 2160
ggtgtttact ctgagcctcg ccccattggc actcgttacc tcacccgtaa tctgtaa 2217
<210>23
<211>2217
<212>DNA
<213>新AAV血清型,克隆rh.50
<400>23
atggctgccg atggttatct tccagattgg ctcgaggaca acctctctga gggcattcgc 60
gagtggtggg acctgaaacc tggagccccg aaacccaaag ccaaccagca aaagcaggac 120
gacggccggg gtctggtgct tcctggctac aagtacctcg gacccttcaa cggactcgac 180
aagggggagc ccgtcaacgc ggcggacgca gcggccctcg agcacgacaa ggcctacgac 240
cagcagctca aagcgggtga caatccgtac ctgcggtata atcacgccga cgccgagttt 300
caggagcgtc tgcaagaaga tacgtctttt gggggcaacc tcgggcgagc agtcttccag 360
gccaagaagc gggttctcga acctctcggt ctggttgagg aaggcgctaa gacggctcct 420
ggaaagaaga gaccggtaga gccgtcacca cagcgttccc ccgactcctc cacgggcatc 480
ggcaagaaag gccagcagcc cgccggaaag agactcaatt tcggtcagac tggcgactca 540
gagtcagtcc ccgaccctca acctatcgga gaacctccag cagcgccctc tagtgtggga 600
tctggtacaa tggctgcagg cggtggcgca ccaatggcag acaataacga aggtgccgac 660
ggagtgggta gttcctcggg aaattggcat tgcgattcca catggctggg cgacagagtc 720
atcaccacca gcacccgaac ctgggccctg cccacctaca acaaccacct ctacaagcaa 780
atctccaacg ggacctcggg aggcagcacc aacgacaaca cctactttgg ctacagcacc 840
ccctgggggt attttgactt taacagattc cactgccact tctcaccacg tgactggcag 900
cgactcatca acaacaactg gggattccgg cccaagagac tcagcttcaa gctcttcaac 960
atccaggtca aagaggtcac gcagaatgaa ggcaccaaga ccatcgccaa taacctcacc 1020
agcaccatcc aggtgtttac ggactcggaa taccagctgc cgtacgtcct cggctctgcc 1080
caccagggct gcctgcctcc gttcccggcg gacgtcttca tgattcctca gtacggctac 1140
ctgactctca acaacggtag tcaggccgtg ggacgttcct ccttctactg cctggagtac 1200
ttcccctctc agatgctgag aacgggcaac aacttttcct tcagctacac tttcgaggac 1260
gtgcctttcc acagcagcta cgcgcacagc cagagtttgg acaggctgat gaatcctctc 1320
gtcgaccagt acctgtacta cctgtcaaga acccagtcta cgggaggcac agcgggaacc 1380
cagcagttgc tgttttctca ggccgggcct agcaacatgt cggctcaggc cagaaactgg 1440
ctgcctggac cctgctacag acagcagcgc gtctccacga cactgtcgca aaacaacaac 1500
agcaactttg cctggactgg tgccaccaag tatcatctga acggcagaga ctctctggtg 1560
aatccgggcg tcgccatggc aaccaacaag gacgacgagg accgcttctt cccatccagc 1620
ggcatcctca tgtttggcaa gcagggagct ggaaaagaca acgtggacta tagcaacgtg 1680
atgctaacca gcgaggaaga aatcaagacc accaaccccg tggccacaga acagtatggc 1740
gtggtggctg ataacctaca gcagcaaaac accgctccta ttgtgggggc cgtcaacagc 1800
cagggagcct tacctggcat ggtctggcag aaccgggacg tgtacctgca gggtcctatt 1860
tgggccaaga ttcctcacac agatggcaac tttcacccgt ctcctttaat gggcggcttt 1920
ggacttaaac atccgcctcc tcagatcctc atcaaaaaca ctcctgttcc tgcggatcct 1980
ccaacagcgt tcaaccaggc caagctgaat tctttcatca cgcagtacag caccggacaa 2040
gtcagcgtgg agatcgagtg ggagctgcag aaggagaaca gcaagcgctg gagcccagag 2100
attcagtata cttccaacta ctacaaatct acaaatgtgg actttgctgt taatactgag 2160
ggtgtttact ctgagcctcg ccccattggc actcgttacc tcacccgtaa tctgtaa 2217
<210>24
<211>2217
<212>DNA
<213>新AAV血清型,克隆hu.39
<400>24
atggctgccg atggttatct tccagattgg ctcgaggaca acctctctga gggcattcgc 60
gagtggtggg acctgaaacc tggagccccg aaacccaaag ccaaccagca aaagcaggac 120
gacggccggg gtctggtgct tcctggctac aagtacctcg gacccttcaa cggactcgac 180
aagggggagc ccgtcaacgc ggcggacgca gcggccctcg agcacgacaa ggcctacgac 240
cagcagctca aagcgggtga caatccgtac ctgcggtata atcacgccga cgccgagttt 300
caggagcgtc tgcaagaaga tacgtctttt gggggcaacc tcgggcgagc agtcttccag 360
gccaagaagc gggttctcga acctctcggt ctggttgagg aaggcgctaa gacggctcct 420
ggaaagaaga gaccggtaga gccgtcacca cagcgttccc ccgactcctc cacgggcatc 480
ggcaagaaag gccagcagcc cgccagaaag agactcaatt tcggtcggac tggcgactca 540
gagtcagtcc ccgaccctca acctatcgga gaacctccag cagcgccctc tagtgtggga 600
tctggtacaa tggctgcagg cggtggcgca ccaatggcag acaataacga aggtgccgac 660
ggagtgggta gttcctcggg aaattggcat tgcgattcca catggctggg cgacagagtt 720
atcaccacca gcacccgaac ctgggccctg cccacctaca acaaccacct ctacaagcaa 780
atctccaacg ggacctcggg aggcagcacc aacgacaaca cctactttgg ctacagcacc 840
ccctgggggt atcttgactt taacagattc cactgccact tctcaccacg tgactggcag 900
cgactcatca acaacaactg gggattccgg cccaagagac tcagcttcaa gctcttcaac 960
atccaggtca aagaggtcac gcagaatgaa ggcaccaaga ccatcgccaa taacctcgcc 1020
agcaccatcc aggtgtttac ggactcggaa taccagccgc cgtacgtcct cggctctgcc 1080
caccagggct gcctgcctcc gttcccggcg gacgtcttca tgattcctca gtacggctac 1140
ctgactctca acaacggtag tcaggccgtg ggacgttcct ccttctactg cctggagtac 1200
ttcccctctc agatgctgag aacgggcaac aacttttcct tcagctacac tttcgaggac 1260
gtgcctttcc acagcagcta cgcgcacagc cagagtttgg acaggctgat gaatcctctc 1320
atcgaccagt acctgtacta cctgtcaaga acccagtcta cgggaggcac agcgggaacc 1380
cagcagttgc tgttttctcg ggccgggcct agcaacatgt cggctcaggc cagaaactgg 1440
ctgcctggac cctgctacag acagcagcgc gtctccacga cactgtcgca aaacaacaac 1500
agcaactttg cctggactgg tgccaccaag tatcatctga acggcagaga ctctctggtg 1560
aatccgggcg tcgccatggc aaccaacaag gacgacgagg accgcttctt cccatccagc 1620
ggcatcctca tgtttggcaa gcagggagct ggaaaagaca acgtggacta tagcaacgtg 1680
atgctaacca gcgaggaaga aatcaagacc accaaccccg tggccacaga acagtatggc 1740
gtggtggctg ataacctaca gcagcaaaac accgctccta ctgtgggggc cgtcaacagc 1800
cagggagcct tacctggcat ggtctggcag aaccgggacg tgtacctgca gggtcctatt 1860
tgggccaaga ttcctcacac agatggcaac tttcacccgt ctcctttaat gggcggcttt 1920
ggacttaaac atccgcctcc tcagatcctc atcaaaaaca ctcctgttcc tgcggatcct 1980
ccaacagcgt tcaaccaggc caagctgaat tctttcatcg cgcagtacag caccggacaa 2040
gtcagcgtgg agatcgagtg ggagctgcag aaggagaaca gcaagcgctg gaacccagag 2100
attcagtata cttccaacta ctacaaatct acaaatgcgg actttgctgt taatactgag 2160
ggtgtttact ctgagcctcg ccccattggc actcgttacc tcacccgtaa tctgtaa 2217
<210>25
<211>2217
<212>DNA
<213>新AAV血清型,克隆rh.49
<400>25
atggctgccg atggttatct tccagattgg ctcgaggaca acctctctga gggcattcgc 60
gagtggtggg acctgaaacc tggagccccg aaacccaaag ccaaccagca aaagcaggac 120
gacggccggg gtctggtgct tcctggctac aagtacctcg gacccttcaa cggactcgac 180
aagggggagc ccgtcaacgc ggcggacgca gcggccctcg agcacgacaa ggcctacgac 240
cagcagctca aagcgggtga caatccgcac ctgcggtata atcacgccga cgccgagttt 300
caggagcgtc tgcaagaaga tacgtctttt gggggcaacc tcgggcgagc agtcttccag 360
gccaagaagc gggttctcga acctctcggt ctggttgagg aaggcgctaa gacggctcct 420
ggaaagaaga gaccggtaga gccgtcacca cagcgttccc ccgactcctc cacgggcatc 480
ggcaagaaag gccagcagcc cgccagaaag agactcaatt tcggtcagac tggcgactca 540
gagtcagtcc ccgaccctca acttatcgga gaacctccag cagcgccctc tagtgtggga 600
tctggtacaa tggctgcagg cggtggcgca ccaatggcag acaataacga aggtgccgac 660
ggagtgggta gttcctcggg aaattggcat tgcgattcca catggctggg cgacagagtc 720
atcaccacca gcacccgaac ctgggccctg cccacctaca acaaccacct ctacaagcaa 780
atctccaacg ggacctcggg aggcagcacc aacgacaaca cctactttgg ctacagcacc 840
ccctgggggt attttgactt taacagattc cactgccact tctcaccacg tgactggcag 900
cgactcatca acaacaactg gggattccgg cccaagagac tcagcttcaa gctcttcaac 960
atccaggtca aagaggtcac gcagaatgaa ggcaccaaga ccatcgccaa taacctcacc 1020
agcaccatcc aggtgtttac ggactcggag taccagctgc cgtacgtcct cggctctgcc 1080
caccagggct gcctgcctcc gttcccggcg gacgtcttca tgattcctca gtacggcaac 1140
ctgactctca acaacggtag tcaggccgtg ggacgttcct ccttctactg cctggagtac 1200
ttcccctctc agatgctgag aacgggcaac aacttttcct tcagctacac tttcgaggac 1260
gtgcctttcc acagcagcta cgcgcacagc cagagtttgg acaggctgat gaatcctctc 1320
atcgaccagt acctgtacta cctgtcaaga acccagtcta cgggaggcac agcgggaacc 1380
cagcagttgc tgttttctca ggccgggcct agcaacatgt cggctcaggc cagaaactgg 1440
ctgcctggac cctgctacag acagcagcgc gtctccacga cactgtcgca aaacaacaac 1500
agcaactttg cctggactgg tgccaccaag tatcatctga acggcagaga ctctctggtg 1560
aatccgggcg tcgccatggc aaccaacaag gacgacgagg accgcttctt cccatccagc 1620
ggcatcctca tgtttggcaa gcagggagct ggaaaagaca acatgggcta tagcaacgtg 1680
atgctaacca gcgaggaaga aatcaagacc accaaccccg tggccacaga acagtatggc 1740
gtggtggctg ataacctaca gcagcaaaac accgctccta ttgtgggggc cgtcaacagc 1800
cagggagcct tacctggcat ggtctggcag aaccgggacg tgtacctgca gggtcctatt 1860
tgggccaaga ttcctcacac agatggcaac tttcacccgt ctcctttaat gggcggcttt 1920
ggacttaaac atccgcctcc tcagatcctc atcaaaaaca ctcctgttcc tgcggatcct 1980
ccaacagcgt tcaaccaggc caagctgaat tctttcatca cgcagtacgg caccggacaa 2040
gtcagcgtgg agatcgagtg ggagctgcag aaggagaaca gcaagcgctg gaacccagag 2100
attcagtata cttccaacta ctacaaatct acaaatgtgg actttgctgt taatactgag 2160
ggtgtttact ctgagcctcg ccccattggc actcgttacc tcacccgtaa tctgtaa 2217
<210>26
<211>2217
<212>DNA
<213>新AAV血清型,克隆rh.57
<400>26
atggctgccg atggttatct tccagattgg ctcgaggaca acctctctga gggcattcgc 60
gagtggtggg cgctgaaacc tggagccccg aagcccaaag ccaaccagca aaagcaggac 120
gacggccggg gtctggtgct tcctggctac aagtacctcg gacccttcaa cggactcgac 180
aagggggagc ccgtcaacgc ggcggacgca gcggccctcg agcacgacaa ggcctacgac 240
cagcagctgc aggcgggtga caatccgtac ctgcggtata atcacgccga cgccgagttt 300
caggagcgtc tgcaagaaga tacgtctttt gggggcaacc tcgggcgagc agtcttccag 360
gccaagaagc gggttctcga acctctcggt ctggttgagg aaggcgctaa gacggctcct 420
ggaaagaaga gaccggtaga gccgtcacca cagcgttccc ccgactcctc cacgggcatc 480
ggcaagaaag gccagcagcc cgccagaaag agactcaatt tcggtcagac tggcgactca 540
gagtcagtcc ccgaccctca acctatcgga gaacctccag cagcgccctc tagtgtggga 600
tctggtacaa tggctgcagg cggtggcgcg ccaatggcag acaataacga aggtgccgac 660
ggagtgggta gttcctcggg aaattggcat tgcgattcca catggctggg cgacagagtc 720
atcaccacca gcacccgaac ctgggccctg cccacctaca acaaccacct ctacaagcaa 780
acctccaacg ggacctcggg aggcagcacc aacgacaaca cctactttgg ctacagcacc 840
ccctgggggt attttgactt taacagattc cactgccact tctcaccacg tgactggcag 900
cgactcatca acaacaactg gggattccgg cccaagagac tcagcttcaa gctcttcaac 960
atccaggtca aagaggtcac gcagaatgaa ggcaccaaga ccatcgccaa taacctcacc 1020
agcaccatcc aggtgtttac ggactcggaa taccagctgc cgtacgtcct cggctctgcc 1080
caccagggct gcctgcctcc gttcccggcg gacgtcttca tgattcctca gtacggctac 1140
ctgactctca acaacggtag tcaggccgtg ggacgttcct ccttctactg cctggagtac 1200
ttcccctctc agatgctgag aacgggcaac aacttttcct tcagctacac tttcgaggac 1260
gtgcctttcc acagcagcta cgcgcacagc cagagtttgg acaggctgat gaatcctctc 1320
atcgaccagt acctgtacta cctgtcaaga acccagtcta cgggaggcac agcgggaacc 1380
cagcagttgc tgttttctca ggccgggcct agcaacatgt cggctcaggc cagaaactgg 1440
ctgcctggac cctgctacag acagcagcgc gtctccacga cactgtcgca aaacaacaac 1500
agcaactttg cctggactgg tgccaccaag tatcatctga acggcagaga ctctctggtg 1560
aatccgggcg tcgccatggc aaccaacaag gacgacgagg accgcttctt cccatccagc 1620
ggcatcctca tgtttggcaa gcagggagct ggaaaagaca acgtggacta tagcaacgtg 1680
atgctaacca gcgaggaaga aatcaagacc accaaccccg tggccacaga acagtatggc 1740
gtggtggctg ataacctaca gcagcaaaac accgctccta ttgtgggggc cgtcaacagc 1800
cagggagcct tacctggcat ggtctggcag aaccgggacg tgtacctgca gggtcctatt 1860
tgggccaaga ttcctcacac agatggcaac tttcacccgt ctcctttaat gggcggcttt 1920
ggacttaaac atccgcctcc tcagatcctc atcaaaaaca ctcctgttcc tgcggatcct 1980
ccaacagcgt tcaaccaggc caagctgaat tctttcatca cgcagtacag caccggacaa 2040
gtcagcgcgg agatcgagtg ggagctgcag aaggagaaca gcaagcgctg gaacccagag 2100
attcagtata cttccaacta ctacaaatct acaaatgtgg actttgctgt taatactgag 2160
ggtgtttact ctgagcctcg ccccattggc actcgttacc tcacccgtaa tctgtaa 2217
<210>27
<211>2217
<212>DNA
<213>新AAV血清型,克隆rh.58
<400>27
atggctgctg acggttatct tccagattgg ctcgaggaca acctctctga gggcattcgc 60
gagtggtggg acctgaaacc tggagccccc aagcccaagg ccaaccagca gaagcaggac 120
gacggccggg gtctggtgct tcctggctac aagtacctcg gacccttcaa cggactcgac 180
aagggggagc ccgtcaacgc ggcggacgca gcggccctcg agcacgacaa ggcctacgac 240
cagcagctca aagcgggtga caatccgtac ctgcggtata atcacgccga cgccgagttt 300
caggagcgtc tgcaagaaga tacgtctttt gggggcaacc tcgggcgagc agtcttccag 360
gccaagaagc gggttctcga acctctcggt ctggttgagg aagctgctaa gacggctcct 420
ggaaagaaga gaccggtaga accgtcacct cagcgttccc ccgactcctc cacgggcatc 480
ggcaagaaag gccagcagcc cgctagaaag agactgaact ttgggcagac tggcgactca 540
gagtcagtcc ccgaccctca accaatcgga gaacctccag cagcgccctc tagtgtggga 600
tctggtacaa tggccgcagg cggtggcgca ccaatggcag acaataacga aggtgccgac 660
ggagtgggta gttcctcggg aaattggcat tgcgattcca catggctggg cgacagagtc 720
atcaccacca gcacccgaac ctgggccctg cccacctaca acaaccacct ctacaagcaa 780
atctccaacg ggacctcggg aggcagcacc aacgacaaca cctactttgg ctacagcacc 840
ccctgggggt attttgactt taacagattc cactgccact tctcaccacg tgactggcag 900
cgactcatca acaacaactg gggattccgg cccaagagac tcagcttcaa gctcttcaac 960
atccaggtca aagaggtcac gcagaatgaa ggcaccaaga ccatcgccaa taacctcacc 1020
agcaccatcc aggtgtttac ggactcggaa taccagctgc cgtacgtcct cggctctgcc 1080
caccagggct gcctgcctcc gttcccggcg gacgtcttca tgattcctca gtacggctac 1140
ctgactctca acaacggtag tcaggccgtg ggacgttcct ccttctactg cctggagtac 1200
ttcccctctc agatgctgag aacgggcaac aacttttcct tcagctacac tttcgaggac 1260
gtgcctttcc acagcagcta cgcgcacagc cagagtttgg acaggctgat gaatcctctc 1320
atcgaccagt acctgtacta cctgtcaaga acccagtcta cgggaggcac agcgggaacc 1380
cagcagctgc tgttttctca ggccgggcct agcaacatgt cggctcaggc cagaaactgg 1440
ctgcctggac cctgctacag acagcagcgc gtctccacga cactgtcgca aaacaacaac 1500
agcaactttg cctggactgg tgccaccaag tatcatctga acggcagaga ctctctggtg 1560
aatccgggcg tcgccatggc aaccaacaag gacgacgagg accgcttctt cccatccagc 1620
ggcatcctca tgtttggcaa gcagggagct ggaaaagaca acgtggacta tagcaacgtg 1680
atgctaacca gcgaggaaga aatcaagacc accaaccccg tggccacaga acagtatggc 1740
gtggtggctg ataacctaca gcagcaaaac accgctccta ttgtgggggc cgtcaacagc 1800
cagggagcct tacctggcat ggtctggcag aaccgggacg tgtacctgca gggtcctatt 1860
tgggccaaga ttcctcacac agatggcaac tttcacccgt ctcctttaat gggcggcttt 1920
ggacttaaac atccgcctcc tcagatcctc atcaaaagca ctcctgttcc tgcggatcct 1980
ccaacagcgt tcaaccaggc caagctgaat tctttcatca cgcagtacag caccggacaa 2040
gtcagcgtgg agatcgagtg ggagctgcag aaggagaaca gcaagtgctg gaacccagag 2100
attcagtata cttccaacta ctacaaatct acaaatgtgg actttgctgt taatactgag 2160
ggtgtttact ctgagcctcg ccccattggc actcgttacc tcacccgtaa tctgtaa 2217
<210>28
<211>2196
<212>DNA
<213>新AAV血清型,克隆pi.1
<400>28
atggctgctg acggttatct tccagattgg ctcgaggaca acctctctga gggcattcgc 60
gagtggtggg cgctgaaacc tggagccccg caacccaaag ccaaccagca aaagcaggac 120
gacggccggg gtctggtgct tcctggctac aagtacctcg gacccttcaa cggactcgac 180
aagggggagc ccgtcaacga ggcggacgcc gcggccctcg agcacgacaa ggcctacgac 240
cagcagctca aagcgggtga caatccgtac ctgcggtata atcacgccga cgccgagttt 300
caagagcgtc tgcaagaaga tacgtccttt gggggcaacc tcgggcgagc agtcttccag 360
gccaaaaaga gggtactcga gcctctgggt ctggttgagg aaggcgctaa gacggctcct 420
ggaaagaagc ggccagtaga accggactcc agctcgggca tcggcaagtc aggccagcag 480
cccgcgaaaa agagactgaa ttttgggcag actggcgact cagagtcagt gcctgacccc 540
caacctctct cagaaccacc cgcaggtccc tctggtctgg gatctggtac aatggctgct 600
ggcggtggcg ctccaatggc agacaataac gaaggcgccg acggagtggg taatgtctca 660
ggaaattggc attgcgattc cacatggctg ggcgaccgag tcatcaccac cagcactcgg 720
acctgggccc tccccaccta caacaaccac ctctacaagc aaatctccaa cgggacctcg 780
ggaggcagca gcaacgacaa cacctacttt ggctacagca ccccctgggg gtattttgac 840
tttaacagat tccactgcca cttttcacca cgtgactggc agcgactcat caacaacaac 900
tgggggttcc ggcccaagaa gctcaacttc aagctcttca acatccaggt caaggaggtc 960
acccagaatg aaggcaccaa gaccatcgcc aataacctca ccagcacggt gcaggtcttt 1020
acggactcgg agtaccagct cccgtacgtg ctcggctctg cccaccaggg ctgcctgcct 1080
ccgttcccgg cggacgtgtt catgattccg cagtacgggt acctgacgct gaacaacggg 1140
agccaggccg tggggcgatc ctccttctac tgcctggagt actttccctc gcagatgctg 1200
agaacgggca acaactttac cttcagctac accttcgagg acgtgccctt ccacagcagc 1260
tacgcgcaca gccagagcct ggaccggctg atgaacccgc tgattgacca gtacctgtac 1320
tacctgtctc ggactcagac caacgggacc aatgccacgc agactctgtt gtttgctcag 1380
gccgggcctc agaacatgtc ggctcaggcc aagaactggc tgcctggtcc ttgctatcgg 1440
cagcagcgcg tctctacgac agtgtcgcaa aacaacaaca gcaactttac ctggaccggg 1500
gcgaccaagt accacctgaa cggccgagac tccctggtga gccccggtgt cgccatggca 1560
acgcacaagg acgacgagga gcgcttcttc ccgagcagcg gggtcctgat gtttggcaag 1620
cagggcgctg gaaaggacaa tgtcgagtac accaacgtga tgctcaccag cgaggaggag 1680
atcaagacca ccaaccctgt ggccacggag cagtacggcg tggtggctga caatctgcag 1740
cagaccaact cagctcccat tgtgggggca gtcaacagcc agggggcctt acccggtatg 1800
gtctggcaga accgggacgt gtacctgcag ggtcccatct gggccaagat cccgcatacg 1860
gacggcaact ttcacccgtc tcctctcatg ggcggctttg gactgaaaca cccgcctccc 1920
cagatcctga tcaaaaacac gccggtacct gcggatcccc cggtgaactt tacggacgct 1980
aagctggcga gtttcatcac gcagtacagc accgggcagg tcagcgtgga gattgagtgg 2040
gagctgcaga aggagaacag caagcgctgg aatcccgaga ttcagtacac ttccaattat 2100
tataaatcag ctaatgtgga ctttgccgtc aatgcagatg gtgtatatag tgaaccccgc 2160
cccattggca ctcgttacct cacccgtaat ctgtaa 2196
<210>29
<211>2196
<212>DNA
<213>新AAV血清型,克隆pi.3
<400>29
atggctgctg acggttatct tccagattgg ctcgaggaca acctctctga gggcattcgc 60
gagtggtggg cgctgaaacc tggagccccg caacccaaag ccaaccagca aaagcaggac 120
gacggccggg gtctggtgct tcctggctac aagtacctcg gacccttcaa cggactcgac 180
aagggggagc ccgtcaacga ggcggacgcc gcggccctcg agcacgacaa ggcctacgac 240
cagcagctca aagcgggtga caatccgtac ctgcggtata atcacgccga cgccgagttt 300
caagagcgtc tgcaagaaga tacgtccttt gggggcaacc tcgggcgagc agtcttccag 360
gccaaaaaga gggtactcga gcctctgggt ctggttgagg aaggcgctaa gacggctcct 420
ggaaagaagc ggccagtaga accggactcc agctcgggca tcggcaagtc aggccagcag 480
cccgcgaaaa agagactgaa ttttgggccg actggcgact cagagtcagt gcctgacccc 540
caacctctct cagaaccacc tgcaggtccc tctggtctgg gatctggtac aatggctgca 600
ggcggtggcg ctccaatggc agacaataac gaaggcgccg acggagtggg taatgtctca 660
ggaaattggc attgcgattc cacatggctg ggcgaccgag tcatcaccac cagcactcgg 720
acctgggccc tccccaccta caacaaccac ctctacaagc aaatctccaa cgggacctcg 780
ggaggcagca gcaacgacaa cacctacttt ggctacagca ccccctgggg gtattttgac 840
tttaacagat tccactgcca cttttcacca cgcgactggc agcgactcat caacaacaac 900
tggggattcc ggcccaagaa gctcaacttc aagctcttca acatccaggt caaggaggtc 960
acccagaatg aaggcaccaa gaccaccgcc aataacctca ccagcacggt gcaggtcttt 1020
acggactcgg agtaccagct cccgtacgtg ctcggctctg cccaccaggg ctgcctgcct 1080
ccgttcccgg cggacgtgtt catgattccg cagtacgggt acctgacgct gaacaacggg 1140
agccaggccg tggggcgatc ctccttctac tgcctggagt actttccctc gcagatgctg 1200
agaacgggca acaactttac cttcagctac accttcgagg acgtgccctt ccacagcagc 1260
tacgcgcaca gccagagcct ggaccggctg atgaacccgc tgattgacca gtacctgtac 1320
tacctgtctc ggactcagac caacgggacc aatgccacgc agactctgtt gtttgctcag 1380
gccgggcctc agaacatgtc ggctcaggcc aagaactggc tgcctggtcc ttgctatcgg 1440
cagcagcgcg tctctacggc agtgtcgcaa aacaacaaca gcaactttac ctggaccggg 1500
gcgaccaagt accacctgaa cggccgagac tccctggtga accccggtgt cgccatggca 1560
acgcacaagg acgacgagga gcgcttcttc ccgagcagcg gggtcctgat gtttggcaag 1620
cagggcgctg gaaaggacaa tgtcgagtac accaacgtga tgctcaccag cgaggaggag 1680
atcaagacca ccaaccctgt ggccacggag cagtacggtg tggtggctga caatctgcag 1740
cagaccaact cggctcccat tgtgggggca gtcaacagcc agggggcctt acccggtatg 1800
gtctggcaga accgggacgt gtacctgcag ggtcccatct gggccaagat cccgcatacg 1860
gacggcaact ttcacccgtc tcctctcatg ggcggctttg gactgaaaca cccgcctccc 1920
cagatcctga tcaaaaacac gccggtacct gcggatcccc cggtgaactt tacggacgct 1980
aagctggcga gtttcatcac gcagtacagc accgggcagg tcagcgtgga gattgagtgg 2040
gagctgcaga aggagaacag caagcgctgg aatcccgaga ttcagtacac ttccaattat 2100
tataaatcag ctaatgtgga ctttgccgtc aatgcagatg gtgtatatag cgaaccccgc 2160
cccattggca ctcgttacct cacccgtaat ctgtaa 2196
<210>30
<211>2196
<212>DNA
<213>新AAV血清型,克隆pi.2
<400>30
atggctgctg acggttatct tccagattgg ctcgaggaca acctctctga gggcattcgc 60
gagtggtggg cgctgaaacc tggagccccg caacccaaag ccaaccagca aaagcaggac 120
gacggccggg gtctggtgct tcctggctac aagtacctcg gacccttcaa cggactcgac 180
aagggggagc ccgtcaacga ggcggacgcc gcggccctcg agcacgacaa ggcctacgac 240
cagcagctca aagcgggtga caatccgtac ctgcggtata atcacgccga cgccgagttt 300
caagagcgtc tgcaagaaga tacgtccttt gggggcaacc tcgggcgagc agtcttccag 360
gccaaaaaga gggtactcga gcctctgggt ctggttgagg aaggcgctaa gacggctcct 420
ggaaagaagc ggccagtaga accggactcc agctcgggca tcggcaagtc aggccggcag 480
cccgcgaaaa agagactgaa ttttgggcag actggcgact cagagtcagt gcctgacccc 540
caacctctct cagaaccacc cgcaggtccc tctggtctgg gatctggtac aatggctgca 600
ggcggtggcg ctccaatggc agacaataac gaaggcgccg acggagtggg taatgcctca 660
ggaaattggc attgcgattc cacatggctg ggcgaccgag tcatcaccac cagcactcgg 720
acctgggccc tccccaccta caacaaccac ctctacaagc aaatctccaa cgggacctcg 780
ggaggcagca gcaacgacaa cacctacttt ggctacagca ccccctgggg gtattttgac 840
tttaacagat tccactgcca cttttcacca cgtgactggc agcgactcat caacaacaac 900
tggggattcc ggcccaagag gctcaacttc aagctcttca acatccaggt caaggaggtc 960
acccagaatg aaggcaccaa gaccatcgcc aataacctca ccagcacggt gcaggtcttt 1020
acggactcga agtaccagct cccgtacgtg ctcggctctg cccaccaggg ctgcctgcct 1080
ccgttcccgg cggacgtgtt catgattccg cagtacgggt acctgacgct gaacaacggg 1140
agccaggccg tggggcgatc ctccttctac tgcctggagt actttccctc gcagatgctg 1200
agaacgggca acaactttac cttcagctac accttcgagg acgtgccctt ccacagcagc 1260
tacgcgcaca gccagagcct ggaccggctg atgaacccgc tgattgacca gtacctgtac 1320
tacctgtctc ggactcagac caacgggacc aatgccacgc agactctgtt gtttgctcag 1380
gccgggcctc agaacatgtc ggctcaggcc aagaactggc tgcctggtcc ttgctatcgg 1440
cagcagcgcg tctctacgac agtgtcgcaa aacaacaaca gcaactttac ctggaccggg 1500
gcgaccaagt accacctgaa cggccgagac tccctggtga accccggtgt cgccatggca 1560
acgcacaagg acgacgagga gcgcttcttc ccgagcagcg gggtcctgat gtttggcaag 1620
cagggcgctg gaaaggacaa tgtcgagtac accaacgtga tgctcaccag cgaggaggag 1680
atcaagacca ccaaccctgt ggccacggag cagtacggtg tggtggctga caatctgcag 1740
cagaccaact cggctcccat tgtgggggca gtcaacagcc agggggcctt acccggtatg 1800
gtctggcaga accgggacgt gtacctgcag ggtcccatct gggccaagat cccgcatacg 1860
gacggcaact ttcacccgtc tcctctcatg ggcggctttg gactgaaaca cccgcctccc 1920
cagatcctga tcaaaaacac gccggtacct gcggatcccc cggtgaactt tacggacgct 1980
aagctggcga gtttcatcac gcagtacagc accgggcagg tcagcgtgga gattgagtgg 2040
gagctgcaga aggagaacag caagcgctgg aatcccgaga ttcagtacac ttccaattat 2100
tataaatcag ctaatgtgga ctttgccgtc aatgcagatg gtgtatatag tgaaccccgc 2160
cccattggca ctcgttacct cacccgtaat ctgtaa 2196
<210>31
<211>2208
<212>DNA
<213>新AAV血清型,克隆rh.60
<400>31
atggctgccg atggttatct tccagattgg ctcgaggaca acctctctga gggcattcac 60
gagtggtggg acccgaaacc tggagccccg aaacccaaag ccaaccagca aaagcaggac 120
gacggccggg gtctggtgct tcctggctac aagtacctcg gacccttcaa cggactcgac 180
aagggggagc ccgtcaacgc ggcggacgca gcggccctcg agcacgacaa ggcctacgac 240
cagcagctca aagcgggtga caatccgtac ctgcggtata atcacgccga cgccgagttt 300
caggagcgtc tgcaagaaga tacgtctttt gggggcaacc tcgggcgagc agtcttccag 360
gccaagaagc gggttctcga acctctcggt ctggttgagg aaggcgctaa gacggctcct 420
ggaaagaaga gaccggtaga gccgtcacca cagcgttccc ccgactcctc cacgggcatc 480
ggcaagaaag gccagcagcc cgccagaaag agactcaatt tcggtcagac tggcgactca 540
gagtcagtcc ccgaccctca acctatcgga gaacctccag cagcgccctc tagtgtggga 600
tctggtacaa tggctgcagg cggtggcgca ccaatggcag acaataacga aggtgccgac 660
ggagtgggta gttcctcggg aaattggcat tgcgattcca catggctggg cgacagagtc 720
atcaccacca gcacccgaac ctgggccctg cccacccaca acaaccacct ctacaagcaa 780
atctccaacg ggacctcggg aggcagcacc aacgacaacg tctacttcgg ctacagcacc 840
ccctgggggt attttgactt caacagattc cactgtcact tctcaccacg tgaccggcag 900
cgactcatca acaacaactg gggattccgg cccaagagac tcagcttcaa gctcttcaac 960
atccaggtca aagaggtcac gcagaatgaa ggcaccaaga ccatcgccaa taacctcacc 1020
agcaccatcc aggtgtttac ggactcggaa taccagctgc cgtacgtcct cggctctgcc 1080
caccagggct gcctgcctcc gttcccggcg gacgtcttca tgattcctca gtacggctac 1140
ctgactctca acaacggtag tcaggccgtg ggacgttcct ccttctactg cctggagtac 1200
ttcccctctc agatgctgag aacgggcaac aacttttcct tcagctacac tttcgaggac 1260
gtgcctttcc acagcagcta cgcgcacagc cagagtttgg acaggctgat gaatcctctc 1320
atcgaccagt acctgtacta cctgtcaaga acccagtcta cggaaggcac agcgggaacc 1380
cagcagttgc tgttttctca ggccgggcct agcaacatgt cggctcaggc cagaaactgg 1440
ctgcctggac cctgctacag acagcagcgc gtctccacga cactgtcgca aaacaacaac 1500
agcaactttg cctggactgg tgccaccaag tatcatctga acggcagaga ctctctggtg 1560
aatccgggcg tcgccatggc aacccacaag gacgacgagg aacgcttctt cccttcgagc 1620
ggagtcctga tttttggaaa aactggagca gctaataaga ctacactgga aaatgtgtta 1680
atgacaaatg aagaggaaat tcgtcctacc aacccggtag ccaccgagga atacgggact 1740
gttagcagca acctgcaggc ggctaacact gcagcccaga cacaagttgt caacaaccag 1800
ggagccttac ctggtatggt ctggcagaac cgggacgtgt acctgcaggg tcccatctgg 1860
gccaagattc ctcacacgga cggcaacttt cacccgtctc cgctgatggg cggctttgga 1920
ctgaagcatc ctcagatcct gatcaaaaac actcctgttc ctgctaatcc cccggaggtg 1980
tttacgcctg ccaagtttgc ttctttcatc acacagtaca gcaccggcca ggtcagcgtg 2040
gagatcgagt gggagctgca gaaggagaac agcaagcgct ggaacccaga gattcagtat 2100
acctccaatt ttgacaaaca gactggtgtg gactttgccg ttgacagcca gggtgtttat 2160
tctgagcctc gccccattgg tactcgttac ctcacccgta atctgtaa 2208
<210>32
<211>2214
<212>DNA
<213>新AAV血清型,克隆rh.48
<400>32
atggctgctg acggttatct tccagattgg ctcgaggaca acctctctga gggcattcgc 60
gagtggtggg acctgaaacc tggagccccc aagcccaagg ccaaccagca gaagcaggac 120
gacggccggg gtctggtgct tcctggctac aagtacctcg gacccttcaa cggactcgac 180
aagggggagc ccgtcaacgc ggcggacgca gcggccctcg agcacgacaa ggcctacgac 240
cagcagctca aagcgggtga caatccgtac ctgcggtata accacgccga cgccgagttt 300
caggagcgtc tgcaagaaga tacgtctttt gggggcaacc tcgggcgagc agtcttccag 360
gccaagaagc gggttctcga acctctcggt ctggttgagg aagctgctaa gacggctcct 420
ggaaagaaga gaccggtaga accgtcacct cagcgttccc ccgactcctc cacgggcatc 480
ggcaagaaag gccagcagcc cgctagaaag agactgaact ttgggcagac tggcgactca 540
gagtcagtcc ccgaccctca accaatcgga gaaccaccag caggcccctc tggtctggga 600
tctggtacaa tggctgcagg cggtggcgca ccaatggctg acaataacaa gggcgccgac 660
ggagtgggta atgcctcagg aaattggcat tgcgattcca catggctggg cgacagagtc 720
atcaccacca gcacccgaac ctgggctttg cccacctaca acaaccacct ctacaagcaa 780
atctccagtc agtcagcagg tagcaccaac gacaacgtct acttcggcta cagcaccccc 840
tgggggtatt ttgacttcaa cagattccac tgtcacttct caccacgtga ctggcagcgg 900
ctcatcaaca gcaactgggg attccggccc aagaagctca acttcaagct gttcaacatc 960
caggtcaagg aggtcacaac gaatgacggc gtcacgacca tcgccaataa ccttaccagc 1020
acggttcagg tcttttcgga ctcggaatac cagctgccct acgtcctcgg ctccgcacac 1080
cagggctgcc tgcctccgtt cccggcggac gtcttcatga ttccccagta cggctacctg 1140
actctgaaca atggcagcca atcggtgggt cgttcctctt tctactgcct ggaatatttc 1200
ccttctcaaa tgctgagaac gggcaacaac ttcaccttca gctacacctt cgaggacgtt 1260
cccttccaca gcagctacgc acacagccag agcctggacc ggctgatgaa tcctcttatc 1320
gaccagtacc tgtattacct ggccagaaca cagagcaacg caggaggcac agctggcaat 1380
cgggaactgc agttttatca gggcgggcct accaccatgg ccgaacaagc caaaaactgg 1440
ctgcctggac cttgcttccg gcaacaaaga gtctccaaga cgctggatca aaacaacaac 1500
agcaactttg cttggactgg tgccaccaaa taccatctaa atggaagaaa ttcattggtt 1560
aatcccggtg tcgccatggc aacccacaag gacgacgagg aacgcttctt cccttcgagc 1620
ggagtcctga tttttggaaa aactggagca gctaataaga ctacactgga aaatgtgtta 1680
atgacaaatg aagaggaaat tcgtcctacc aacccggtag ccaccgagga atacgggact 1740
gttagcagca acctgcaggc ggctaacact gcagcccaga cacaagttgt caacaaccag 1800
ggagccttac ctggtatggt ctggcagaac cgggacgtgt acctgcaggg tcccatctgg 1860
gccaagattc ctcacacgga cggcaacttt cacccgtctc cgctgatggg cggctttgga 1920
ctgaagcatc cgcctcctca gatcctgatc aaaaacactc ctgttcctgc taatcccccg 1980
gaggtgttta cgcctgccaa gtttgcttct ttcatcacac agtacagcac cggccaggtc 2040
agcgtggaga tcgagtggga gctgcagaag gagaacagca agcgctggaa cccagagatt 2100
cagtatacct ccaattttga caaacagact ggtgtggact ttgccgttga cagccagggt 2160
gtttattctg agcctcgccc cattggtact cgttacctca cccgtaatct gtaa 2214
<210>33
<211>2214
<212>DNA
<213>新AAV血清型,克隆rh.62
<400>33
atggctgctg acggttatct tccagattgg ctcgaggaca acctctctga gggcattcgc 60
gagtggtggg acctgaaacc tggagccccc aagcccaagg ccaaccagca gaagcaggac 120
gacggccggg gtctggtgct tcctggctac aagtacctcg gacccttcaa cggactcgac 180
aagggggagc ccgtcaacgc ggcggacgca gcggccctcg agcacgacaa ggcctacgac 240
cagcagctca aagcgggtga caatccgtac ctgcggtata accacgccga cgccgagttc 300
caggagcgtc tgcaagaaga tacgtctttt gggggcaacc tcgggcgagc agtcttccag 360
gccaagaagc gggttctcga acctctcggt ctggctgagg aagctgctaa gacggctcct 420
ggaaagaaga gaccggtaga accgtcacct cagcgttccc ccgactcctc cacgggcatc 480
ggcaagaaag gccagcagcc cgctagaaag agactgaact ttgggcagac tggcgactca 540
gagtcagtcc ccgaccctca accaatcgga gagccaccag caggcccctc tggtctggga 600
tctggtacaa tggctgcagg cggtggcgca ccaatggctg acaataacaa gggcgccgac 660
ggagtgggta atgcctcagg aaattggcat tgcgattcca catggctggg cgacagagtc 720
atcaccacca gcacccgaac ctgggctttg cccacctaca acaaccacct ctacaagcaa 780
atctccagtc agtcagcagg tagcaccaac gacaacgtct acttcggcta cagcaccccc 840
tgggggtatt ttgacttcaa cagattccac tgtcacttct caccacgtga ctggcagcgg 900
ctcatcaaca acaactgggg attccggccc aagaagctca acttcaagct gttcaacatc 960
caggtcaagg aggtcacaac gggtgacggc gtcacgacca tcgccaataa ccttaccagc 1020
acggttcagg tcttttcgga ctcggaatac cagctgccct acgtcctcgg ctccgcacac 1080
cagggctgcc tgcctccgtt cccggcggac gtcttcatga ttccccagta cggctacctg 1140
actctgaaca atgacagcca atcggtgggt cgttcctctt tctactgcct ggaatatttc 1200
ccttctcaaa tgctgagaac gggcaacaac ttcaccttca gctacacctt cgaggacgtt 1260
cccttccaca gcagctacgc acacagccag agcctggacc ggctgatgaa tcctcttatc 1320
gaccagtacc tgtattacct ggccagaaca cagagcaacg caggaggcac agctggcaat 1380
cgggaactgc agttttatca gggcgggcct accaccatgg ccgaacaagc caaaaactgg 1440
ctgcctggac cttgcttccg gcaacaaaga gtctccaaga cgctggatca aaacaacaac 1500
agcaactttg cttggactgg tgccaccaaa taccatctaa atggaagaaa ttcattggtt 1560
aatcccggtg tcgccatggc aacccacaag gacgacgagg aacgcttctt cccttcgagc 1620
ggagtcctga tttttggaaa aactggagca gctaataaga ctacactgga aaatgtgtta 1680
atgacaaatg aagaggaaat tcgtcctacc aacccggtag ccaccgagga atacgggact 1740
gttagcagca acctgcaggc ggctaacact gcagcccaga cacaagttgt caacaaccag 1800
ggagccttac ctggtatggt ctggcagaac cgggacgtgt acctgcaggg tcccatctgg 1860
gccaagattc ctcacacgga cggcaacttt cacccgtctc cgctgatggg cggctttgga 1920
ctgaagcatc cgcctcctca gatcctgatc aaaaacactc ctgttcctgc taatcccccg 1980
gaggtgttta cgcctgccaa gtttgcttct ttcatcacac agtacagcac cggccaggtc 2040
agcgtggaga tcgagtggga gctgcagaag gagaacagca agcgctggaa cccagagatt 2100
cagtatacct ccaattttga caaacagact ggtgtggact ttgccgttga cagccagggt 2160
gtttattctg agcctcgccc cattggtact cgttacctca cccgtaatct gtaa 2214
<210>34
<211>2214
<212>DNA
<213>新AAV血清型,克隆rh.44
<400>34
atggctgctg acggttatct tccagattgg ctcgaggaca acctctctga gggcattcgc 60
gagtggtggg acctgaaacc tggagccccc aagcccaagg ccaaccagca gaagcaggac 120
gacggccggg gtctggtgct tcctggctac aagtacctcg gacccttcaa cggactcgac 180
aagggggagc ccgtcagcgc ggcggacgca gcggccctcg agcacgacaa ggcctgcgac 240
cagcggctca aagcgggtga caatccgtac ctgcggtata accacgccga cgccgagttt 300
caggagcgtc tgcaagaaga tacgtctttt gggggcaacc tcgggcgagc agtcttccag 360
gccaagaagc gggttctcga acctctcggt ctggttgagg aagctgctaa gacggctcct 420
ggaaagaagg gaccggtaga accgtcacct cagcgttccc ccgactcctc cacgggcatc 480
ggcaagaaag gccagcagcc cgctagaaag agactgaact ttgggcagac tggcgactca 540
gagtcagtcc ccgaccctca accaatcgga gaaccaccag caggcccctc tggtctggga 600
tctggtacaa tggctgcagg cggtggcgca ccaatggctg acaataacga gggcgccgac 660
ggagtgggta atgcctcagg aaattggcat tgcgattcca catggctggg cgacagagtc 720
atcaccacca gcacccgaac ctgggctttg cccacctaca acaatcacct ctacaagcaa 780
atctccagtc agtcagcagg tagcaccaac gacaacgtct acttcggcta cagcaccccc 840
tgggggtatt ttgacttcaa cagattccac tgtcacttct caccacgtga ctggcagcgg 900
ctcatcaaca acaactgggg attccggccc aagaagctca acttcaagct gttcaacatc 960
caggtcaagg aggtcacaac gaatgacggc gtcacgacca tcgccaataa ccttaccagc 1020
acggttcagg tcttttcgga ctcggaatac cagctgccct acgtcctcgg ctccgcacac 1080
cagggctgcc tgcctccgtt cccggcggac gtcttcatga ttccccagta cggctacctg 1140
actctgaaca atggcagcca atcggtgggt cgttcctctt tctactgcct ggaatatttc 1200
ccttctcaaa tgctgagaac gggcaacaac ttcaccttca gctacacctt cgaggacgtt 1260
cccttccaca gcagctacgc acacagccag agcctggacc ggctgatgaa tcctcttatc 1320
gaccagtacc tgtattacct ggccagaaca cagagcaacg caggaggcac agctggcaat 1380
cgggaactgc agttttatca gggcgggcct accaccatgg ccgaacaagc caaaaactgg 1440
ctgcctggac cttgcttccg gcaacaaaga gtctccaaga cgctggatca aaacaacaac 1500
agcaactttg cttggactgg tgccaccaaa taccatctaa atggaagaaa ttcattggtt 1560
aatcccggtg tcgccatggc aacccacaag gacgacgagg aacgcttctt cccttcgagc 1620
ggagtcctga tttttggaaa aactggagca gctaataaga ctacactgga aaatgtgtta 1680
atgacaaatg aagaggaaat tcgtcctacc aacccggtag ccaccgagga atacgggact 1740
gttagcagca acctgcaggc ggctaacact gcagcccaga cacaagttgt caacaaccag 1800
ggagccttac ctggtatggt ctggcagaac cgggacgtgt acctgcaggg tcccatctgg 1860
gccaagattc ctcacacgga cggcaacttt cacccgtctc cgctgatggg cggctttgga 1920
ctgaagcatc cgcctcctca gatcctgatc aaaaacactc ctgttcctgc taatcccccg 1980
gaggtgttta cgcctgccaa gtttgcttct ttcatcacac agtacagcac cggccaggtc 2040
agcgtggaga tcgagtggga gctgcagaag gagaacagca agcgctggaa cccagagatt 2100
cagtatacct ccaattttga cgaacagact ggtgtggact ttgccgttga cagccagggt 2160
gtttattctg agcctcgccc cattggtact cgttacctca cccgtaatct gtaa 2214
<210>35
<211>2214
<212>DNA
<213>新AAV血清型,克隆rh.65
<400>35
atggctgctg acggttatct tccagattgg ctcgaggaca acctctctga gggcattcgc 60
gagtggtggg acctgaaacc tggagccccc aagcccaagg ccaaccagca gaagcaggac 120
gacggccggg gtctggtgct tcctggctac aagtacctcg gacccttcaa cggactcgac 180
aagggggagc ccgtcaacgc ggcggacgca gcggccctcg agcacgacaa ggcctacgac 240
cagcagctca aagcgggtga caatccgtac ctgcggtata accacgccga cgccgagttt 300
caggagcgtc tgcaagaaga tacgtctttt gggggcaacc tcgggcgagc agtcttccag 360
gccaagaagc gggttctcga acctctcggt ctggttgagg aagctgctaa gacggctcct 420
ggaaagaaga gaccggtaga accgtcacct cagcgttccc ccgactcctc cacgggcatc 480
ggcaagaaag gccagcagcc cgctagaaag agactgaact ttgggcagac tggcgactca 540
gagtcagtcc ccgaccctca accaatcgga gaaccaccag caggcccctc tggtctggga 600
tctggtacaa tggctgcagg cggtggcgct ccaatggcag acaataacga aggcgccgac 660
ggagtgggta atgcctcagg aaattggcat tgcgattcca catggctggg cgacagagtc 720
atcaccacca gcacccgaac ctgggctttg cccacctaca acaaccacct ctacaagcaa 780
atctccagtc agtcagcagg tagcaccaac gacaacgtct acttcggcta cagcaccccc 840
tgggggtatt ttgacttcaa cagattccac tgtcacttct caccacgtga ctggcagcgg 900
ctcatcaaca acaactgggg attccggccc aagaagctca acttcaagct gttcaacatc 960
caggtcaagg aggtcacaac gaatgacggc gtcacgacca tcgccaataa ccttaccagc 1020
acggttcagg tcttttcgga ctcggaatac cagctgccct acgtcctcgg ctccgcacac 1080
cagggctgcc tgcctccgtt cccggcggac gtcttcatga ttccccagta cggctacctg 1140
actctgaaca atggcagcca atcggtgggt cgttcctctt tctactgcct ggaatatttc 1200
ccttctcaaa tgctgagaac gggcaacaac ttcaccttca gctacacctt cgaggacgtt 1260
cccttccaca gcagctacgc acacagccag agcctggacc ggctgatgaa tcctcttatc 1320
gaccagtacc tgtattacct ggccagaaca cagagcaacg caggaggcac agctggcaat 1380
cgggaactgc agttttatca gggcgggcct accaccatgg ccgaacaagc caaaaactgg 1440
ctgcctagac cttgcttccg gcaacaaaga gtctccaaga cgctggatca aaacaacaac 1500
agcaactttg cttggactgg tgccaccaaa taccatctaa atggaagaaa ttcattggtt 1560
aatcccggtg tcgccatggc aacccacaag gacgacgagg aacgcttctt cccttcgagc 1620
ggagtcctga tttttggaaa aactggagca gctaataaga ctacactgga aaatgtgtta 1680
atgacaaatg aagaggaaat tcgtcctacc aacccggtag ccaccgagga atacgggact 1740
gttagcagca acctgcaggc ggctaacact gcagcccaga cacaagttgt caacaaccag 1800
ggagccttac ctggtatggt ctggcagaac cgggacgtgt acctgcaggg tcccatctgg 1860
gccaagattc ctcacacgga cggcaacttt cacccgtctc cgctgatggg cggctttgga 1920
ctgaagcatc cgcctcctca gatcctgatc aaaaacactc ctgttcctgc taatcccccg 1980
gaggtgttta cgcctgccaa gtttgcttct ttcatcacac agtacagcac cggccaggtc 2040
agcgtggaga tcgagtggga gctgcagaag gagaacagca agcgctggaa cccagagatt 2100
cagtatacct ccaattttga caaacagact ggtgtggact ttgccgttga cagccagggt 2160
gtttattctg agcctcgccc cattggtact cgttacctca cccgtaatct gtaa 2214
<210>36
<211>2214
<212>DNA
<213>新AAV血清型,克隆rh.67
<400>36
atggctgctg acggttatct tccagattgg ctcgaggaca acctctctga gggcattcgc 60
gagtggtggg acctgaaacc tggagccccc aagcccaagg ccaaccagca gaagcaggac 120
gacggccggg gtctggtgct tcctggctac aagtacctcg gacccttcaa cggactcgac 180
aagggggagc ccgtcaacgc ggcggacgca gcggccctcg agcacgacaa ggcctacgac 240
cagcagctca aagcgggtga caatccgtac ctgcggtata accatgccga cgccgagttt 300
caggagcgtc tgcaagaaga tacgtctttt gggggcaacc tcgggcgagc agtcctccag 360
gccaagaagc gggttctcga acctctcggt ctggttgagg aagctgctaa gacggctcct 420
ggaaagaaga gaccggtaga accgtcacct cagcgttccc ccgactcctc cacgggcatc 480
ggcaagaaag gccagcagcc cgctagaaag agactgaact ttgggcagac tggcgactca 540
gagtcagtcc ccgaccctca accaatcgga gaaccaccag caggcccctc tggtctggga 600
tctggtacaa tggctgcagg cggtggcgct ccaatggcag acaataacga aggcgccgac 660
ggagtgggta atgcctcagg aaattggcat tgcgattcca catggctggg cgacagagtc 720
atcaccacca gcacccgaac ctgggctttg cccacctaca acaaccacct ctacaagcaa 780
atctccagtc agtcagcagg tagcaccaac gacaacgtct acttcggcta cagcaccccc 840
tgggggtatt ttgacttcaa cagattccac tgtcacttct caccacgtga ctggcagcgg 900
ctcatcaaca acaactgggg attccggccc aagaagctca acttcaagct gttcaacatc 960
caggtcaagg aggtcacaac gaatgacggc gtcacgacca tcgccaataa ccttaccagc 1020
acggttcagg tcttttcgga ctcggaatac cagctgccct acgtcctcgg ctccgcacac 1080
cagggctgcc tgcctccgtt cccggcggac gtcttcatga ttccccagta cggctacctg 1140
actctgaaca atggcagcca atcggtgggt cgttcctctt tctactgcct ggaatatttc 1200
ccttctcaaa tgctgagaac gggcaacaac ttcaccttca gctacacctt cgaggacgtt 1260
cccttccaca gcagctacgc acacagccag agcctggacc ggctgatgaa tcctcttatc 1320
gaccagtacc tgtattacct ggccagaaca cagagcaacg caggaggcac agctggcaat 1380
cgggaactgc agttttatca gggcgggcct accaccatgg ccgaacaagc caaaaactgg 1440
ctgcctggac cttgcttccg gcaacaaaga gtctccaaga cgctggatca aaacaacaac 1500
agcaactttg cttggactgg tgccaccaaa taccatctaa atggaagaaa ttcattggtt 1560
aatcccggtg tcgccatggc aacccacaag gacgacgagg aacgcttctt cccttcgagc 1620
ggagtcctga tttttggaaa aactggagca gctaataaga ctacactgga aaatgtgtta 1680
atgacaaatg aagaggaaat tcgtcctacc aacccggtag ccaccgagga atacgggact 1740
gttagcagca acctgcaggc ggctaacact gcagcccaga cacaagttgt caacaaccag 1800
ggagccttac ctggtatggt ctggcagaac cgggacgtgt acctgcaggg tcccatctgg 1860
gccaagattc ctcacacgga cggcaacttt cacccgtctc cgctgatggg cggctttgga 1920
ctgaagcatc cgcctcctca gatcctgatc aaaaacactc ctgttcctgc taatcccccg 1980
gaggtgttta cgcctgccaa gtttgcttct ttcatcacac agtacagcac cggccaggtc 2040
agcgtggaga tcgagtggga gctgcagaag gagaacagca agcgctggaa cccagagatt 2100
cagtatacct ccaattttga caaacagact ggtgtggact ttgccgttga cagccagggt 2160
gtttattctg agcctcgccc cattggtact cgttacctca cccgtaatct gtaa 2214
<210>37
<211>2214
<212>DNA
<213>新AAV血清型,克隆rh.55
<400>37
atggctgccg atggttatct tccagattgg ctcgaggaca acctctctga gggcattcgc 60
gagtggtggg acctgaaacc tggagccccg aaacccaaag ccaaccagca aaagcaggac 120
gacggccggg gtctggtgct tcctggctac aagtacctcg gacccttcaa cggactcgac 180
aagggggagc ccgtcaacgc ggcggacgca gcggccctcg agcacgacaa ggcctacgac 240
cagcagctca aagcgggtga caatccgtac ctgcggtata atcacgccga cgccgagttt 300
caggagcgtc tgcaagaaga tacgtctttt gggggcaacc tcgggcgagc agtcttccag 360
gccaagaagc gggttctcga acctctcggt ctggttgagg aaggcgctaa gacggctcct 420
ggaaagaaga gaccggtaga gccgtcacca cagcgttccc ccgactcctc cacgggcatc 480
ggcaagaaag gccagcagcc cgccagaaag agactcaatt tcggtcagac tggcgactca 540
gagtcagtcc ccgaccctca acctatcgga gaacctccag cagcgccctc tagtgtggga 600
tctggtacaa tggctgcagg cggtggcgca ccaatggcag acaataacga aggtgccgac 660
ggagtgggta gttcctcggg aaattggcat tgcgattcca cacggctggg cgacagagtc 720
atcaccacca gcacccggac ctgggctttg cccacctaca acaaccacct ctacaagcaa 780
atctccagtc agtcagcagg tagcaccaac gacaacgtct acttcggcta cagcaccccc 840
tgggggtatt ttgacttcaa cagattccac tgtcacttct caccacgtga ctggcagcgg 900
ctcatcaaca acaactgggg attccggccc aagaagctca acttcaagct gttcaacatc 960
caggtcaagg aggtcacaac gaatgacggc gtcacgacca tcgccaataa ccttaccagc 1020
acggttcagg tcttttcgga ctcggaatac cagctgccct acgtcctcgg ctccgcacac 1080
cagggctgcc tgcctccgtt cccggcggac gtcttcatga ttccccagta cggctacctg 1140
actctgaaca atggcagcca atcggtgggt cgttcctctt tctactgcct ggaatatttc 1200
ccttctcaaa tgctgagaac gggcaacaac ttcaccttca gctacacctt cgaggacgtt 1260
cccttccaca gcagctacgc acacagccag agcctggacc ggctgatgaa tcctcttatc 1320
gaccagtacc tgtattacct ggccagaaca cagagcaacg caggaggcac agctggcaat 1380
cgggaactgc agttttatca gggcgggcct accaccatgg ccgaacaagc caaaaactgg 1440
ctgcctggac cttgcttccg gcaacgaaga gtctccaaga cgctggatca aaacaacaac 1500
agcaactttg cttggactgg tgccaccaaa taccatctaa atggaagaaa ttcattggtt 1560
aatcccggtg tcgccatggc aacccacaag gacgacgagg aacgcttctt cccttcgagc 1620
ggagtcctga tttttggaaa aactggagca gctaataaga ctacactgga aaatgtgtta 1680
atgacaaatg aagaggaaat tcgtcctacc aacccggtag ccaccgagga atacgggact 1740
gttagcagca acctgcaggc ggctaacact gcagcccaga cacaagttgt caacaaccag 1800
ggagccttac ctggtatggt ctggcagaac cgggacgtgt acctgcaggg tcccatctgg 1860
gccaagattc ctcacacgga cggcaacttt cacccgtctc cgctgatggg cggctttgga 1920
ctgaagcatc cgcctcctca gatcctgatc aaaaacactc ctgttcctgc taatcccccg 1980
gaggtgttta cgcctgccaa gtttgcttct ttcatcacac agtacagcac cggccaggtc 2040
agcgtggaga tcgagtggga gctgcagaag gagaacagca agcgctggaa cccagagatt 2100
cagtatacct ccaattttga caaacagact ggtgtggact ttgccgttga cagccagggt 2160
gtttattctg agcctcgccc cattggtact cgttacctca cccgtaatct gtaa 2214
<210>38
<211>2217
<212>DNA
<213>新AAV血清型,克隆rh.47
<400>38
atggctgccg atggttatct tccagattgg ctcgaggaca acctctctga gggcattcgc 60
gagtggtggg acctgaaacc tggagccccg aaacccaaag ccaaccagca aaagcaggac 120
gacggccggg gtctggtgct tcctggctac aagcacctcg gacccttcaa cggactcgac 180
aagggggagc ccgtcaacgc ggcggacgca gcggccctcg agcacgacaa ggcctacgac 240
cagcagctca aagcgggtga caatccgtac ctgcggtata atcacgctga cgccgagttt 300
caggagcgtc tgcaagaaga tacgtctttt gggggcaacc tcgggcgagc agtcttccag 360
gccaagaagc gggttctcga acctctcggt ctggttgagg aaggcgctaa gacggctcct 420
ggaaagaaga gaccggtaga gccgtcacca cagcgttccc ccgactcctc cacgggcatc 480
ggcaagaaag gccagcagcc cgccagaaag agactcaatt tcggtcagac tggcgactca 540
gagtcagtcc ccgaccctca acctatcgga gaacctccag cagcgccctc tagtgtggga 600
tctggtacaa tggctgcagg cggtggcgca ccaatggcag acaataacga aggtgccgac 660
ggagtgggta gttcctcggg aaattggcat tgcgattcca catggctggg cgacagagtc 720
atcaccacca gcacccgaac ctgggccctg cccacctaca acaaccacct ctacaagcaa 780
atctccaacg ggacctcggg aggcagcacc aacgacaaca cctactttgg ctacagcacc 840
ccctgggggt attttgactt taacagattc cactgccact tctcaccacg tgactggcag 900
cgactcatca acaacaactg gggattccgg cccaagagac tcagcttcaa gctcttcaac 960
atccaggtca aagaggtcac gcagaatgaa ggcaccaaga ccatcgccaa taaccttacc 1020
agcacggttc aggtcttttc ggactcggaa taccagctgc cctacgtcct cggctccgca 1080
caccagggct gcctgcctcc gttcccggcg gacgtcttca tgattcccca gtacggctac 1140
ctgactctga acaatggcag ccaatcggtg ggtcgttcct ctttctactg cctggaatat 1200
ttcccttctc aaatgctgag aacgggcaac aacttcacct tcagctacac cttcgaggac 1260
gttcccttcc acagcagcta cgcacacagc cagagcctgg accggctgat gaatcctctt 1320
atcgaccagt acctgtatta cctggccaga acacagagca acgcaggagg cacagctggc 1380
aatcgggaac tgcagtttta tcagggcggg cctaccacca tggccgaaca agccaaaaac 1440
tggctgcctg gaccttgctt ccggcaacaa agagtctcca agacgctgga tcaaaacaac 1500
aacagcaact ttgcttggac tggtgccacc aaataccatc taaatggaag aaattcattg 1560
gttaatcccg gtgtcgccat ggcaacccac aaggacgacg aggaacgctt cttcccttcg 1620
agcggagtcc tgatttttgg aaaaactgga gcagctaata agactacact ggaaaatgtg 1680
ttaatgacaa atgaagagga aattcgtcct accaacccgg tagccaccga ggaatacggg 1740
actgttagca gcaacctgca ggcggctaac actgcagccc agacacaagt tgtcaacaac 1800
cagggagcct tacctggtat ggtctggcag aaccgggacg tgtacctgca gggtcccatc 1860
tgggccaaga ttcctcacac ggacggcaac tttcacccgt ctccgctgat gggcggcttt 1920
ggactgaagc atccgcctcc tcagatcctg atcaaaaaca ctcctgttcc tgctaatccc 1980
ccggaggtgt ttacgcctgc caagtttgct tctttcatca cacagtacag caccggccag 2040
gtcagcgtgg agatcgagtg ggagctgcag aaggagaaca gcaagcgctg gaacccagag 2100
attcagtata cctccaattt tgacaaacag actggtgtgg actttgccgt tgacagccag 2160
ggtgtttatt ctgagcctcg ccccattggt actcgttacc tcacccgtaa tctgtaa 2217
<210>39
<211>2214
<212>DNA
<213>新AAV血清型,克隆rh.69
<400>39
atggctgctg acggttatct tccagattgg ctcgaggaca acctctctga gggcattcgc 60
gagtggtggg acctgaaacc tggagccccc aagcccaagg ccaaccagca gaagcaggac 120
gacggccggg gtctggtgct tcctggctac aagtacctcg gacccttcaa cggactcgac 180
aagggggagc ccgtcaacgc ggcggacgca gcggccctcg agcacgacaa ggcctacgac 240
cagcagctca aagcgggtga caatccgtac ctgcggtata accacgccga cgccgagttt 300
caggagcgtc tgcaagaaga tacgtctttt gggggcaacc tcgggcgagc agtcttccag 360
gccaagaagc gggttctcga acctctcggt ctggttgagg aagctgctaa gacggctcct 420
ggaaagaaga gaccggtaga accgtcacct cagcgttccc ccgactcctc cacgggcatc 480
ggcaagaaag gccagcagcc cgctagaaag agactgaact ttgggcagac tggcgactca 540
gagtcagtcc ccgaccctca accaatcgga gaaccaccag caggcccctc tggtctggga 600
tctggtacaa tggctgcagg cggtggcgcc ccaatggcag acaataacga aggcgccgac 660
ggagtgggta atgcctcagg aaattggcat tgcgattcca catggctggg cgacagagtc 720
atcaccacca gcacccgaac ctgggctttg cccacctaca acaaccacct ctacaagcaa 780
atctccagtc agtcagcagg tagcaccaac gacaacgtct acttcggcta cagcaccccc 840
tgggggtatt ttgacttcaa cagattccac tgtcacttct caccacgtga ctggcagcgg 900
ctcatcaaca acaactgggg attccggccc aagaagctca acttcaagct gttcaacatc 960
caggtcaagg aggtcacaac gaatgacggc gtcacgacca tcgccaataa ccttaccagc 1020
acggttcagg tcttttcgga ctcggaatac cagctgccct acgtcctcgg ctccgcacac 1080
cagggctgcc tgcctccgtt cccggcggac gtcttcatga ttccccagta cggctacctg 1140
actctgaaca atggcagcca atcggtgggt cgttcctctt tctactgcct ggaatatttc 1200
ccttctcaaa tgctgagaac gggcaacaac ttcaccatca gctacacctt cgaggacgtt 1260
cccttccaca gcagctacgc acacagccag agcctggacc ggctgatgaa tcctcttatc 1320
gaccagtacc tgtattacct ggccagaaca cagagcaacg caggaggcac agctggcaat 1380
caggaactgc agttttatca gggcgggcct accaccatgg ccgaacaagc caaaaactgg 1440
ctgcctggac cttgcttccg gcaacaaaga gtctccaaga cgctggatca aaacaacaac 1500
agcaactttg cttggactgg tgccaccaaa taccatctaa atggaagaaa ttcattggtt 1560
aatcccggtg tcgccatggc aacccacaag gacgacgagg aacgcttctt cccttcgagc 1620
ggagtcctga tttttggaaa aactggagca gctaataaga ctacactgga aaatgtgtta 1680
atgacaaatg aagaggaaat tcgtcctacc aacccggtag ccaccgagga atacgggact 1740
gttagcagca acctgcaggc ggctaacact gcagcccaga cacaagttgt caacaaccag 1800
ggagccttac ctggtatggt ctggcagaac cgggacgtgt acctgcaggg tcccatctgg 1860
gccaagattc ctcacacaga tggcaacttt cacccgtctc ctttaatggg cggctttgga 1920
cttaaacatc cgcctcctca gatcctcatc aaaaacactc ctgttcctgc ggatcctcca 1980
acagcgttca accaggccaa gctgaattct ttcatcacgc agtacagcac cggacaagtc 2040
agcgtggaga tcgagtggga gctgcagaag gagaacagca agcgctggaa cccagagatt 2100
cagtatactt ccaactacta caaatctaca aatgtggact ttgctgttaa tactgagggt 2160
gtttactctg agcctcgccc cattggcact cgttacctca cccgtaatct gtaa 2214
<210>40
<211>2214
<212>DNA
<213>新AAV血清型,克隆rh.54
<400>40
atggctgctg acggttatct tccagattgg ctcgaggaca acctctctga gggcattcgc 60
gagtggtggg acctgaaacc tggagccccg aaacccaaag ccaaccagca gaagcaggac 120
gacggccggg gtctggtgct tcctggctac aagtacctcg gacccttcaa cggactcgac 180
aagggggagc ccgtcaacgc ggcggacgca gcggccctcg agcacgacaa ggcctacgac 240
cagcagctca aagcgggtga caatccgtac ctgcggtata accacgccga cgccgagttt 300
caggagcgtc tgcaagaaga tacgtctttt gggggcaacc tcgggcgagc agtcttccag 360
gccaagaagc gggttctcga acctctcggt ctggttgagg aagctgctaa gacggctcct 420
ggaaagaaga gaccggtaga accgtcacct cagcgttccc ccgactcctc cacgggcatc 480
ggcaagaaag gccagcagcc cgctagaaag agactgaact ttgggcagac tggcgactca 540
gagtcagtcc ccgaccctca acctctcgga gaaccaccag caggcccctc tggtctggga 600
tctggtacaa tggctgcagg cggtggcgct ccaatggcag acaataacga aggcgccgac 660
ggagtgggta atgcctcagg aaattggcat tgcgattcca catggctggg cgacagagtc 720
atcaccacca gcacccgaac ctgggctttg cccacctaca acaaccacct ctacaagcaa 780
atctccagtc agtcagcagg tagcaccaac gacaacgtct acttcggcta cagcaccccc 840
tgggggtatt ttgacttcaa cagattccac tgtcacttct caccacgtga ctggcagcga 900
ctcatcaaca acaactgggg attccggccc aagaagctca acttcaagct cttcaacatc 960
caagtcaagg aggtcacgac gaatgacggc gtcacgacca tcgctaataa ccttaccagc 1020
acggttcagg tcttttcgga ctcggagtac cagctgccgt acgtcctcgg ctctgcccac 1080
cagggctgcc tgcctccgtt cccggcggac gtcttcatga ttcctcagta cggctacctg 1140
actctgaaca atggcagcca atcggtggga cgttcatcct tctactgcct ggaatacttc 1200
ccttctcagg tgctgagaac gggtaacaac ttcaccttca gttacacctt cgaggacgtg 1260
cctttccaca gcagctacgc gcacagccag agcctagacc ggctgatgaa tcccctcatc 1320
gaccagtacc tgtattacct ggctagaaca cagagtaacc caggaggcac atctggcaat 1380
cgggaactgc agttttacca gggcgggcct tccaccatgg ccgaacaagc caagaactgg 1440
ttacctggac cttgcttccg gcaacaaaga gtttccaaaa cactggatca aaacaacaac 1500
agcaactttg cttggactgg tgccaccaaa tatcacctga acggcagaaa ctcattggtg 1560
aatcctggtg tcgccatggc aactcacaag gacgacgagg accgcttttt cccatccagc 1620
ggagtcctga tttttggaaa aactggagca accaacaaga ctacattgga aaacgtgtta 1680
atgacaaatg aagaagaaat tcgtcctact aatcctgtgg ccacagaaga atacgggata 1740
gtcagcagca atttacaagc ggccaatact gcagcccaga cacaagttgt caacaaccag 1800
ggagccttac ctggcatggt ctggcagaac cgggacgtgt acctgcaggg tcccatctgg 1860
gccaaaattc ctcacacaga cggcaacttt cacccgtctc cgctgatggg cggctttgga 1920
ctgaagcatc cgcctcctca gatcctgatc aaaaacactc ctgttcctgc taatcccccg 1980
gaggtgttta cgcctgccaa gtttgcttct ttcatcacac agtacagcac cggccaggtc 2040
agcgtggaga tcgagtggga gctgcagaag gagaacagca agcgctggaa cccagagatt 2100
cagtatacct ccaattttga caaacagact ggtgtggact ttgccgttga cagccagggt 2160
gtttattctg agcctcgccc cattggtact cgttacctca cccgtaatct gtaa 2214
<210>41
<211>2214
<212>DNA
<213>新AAV血清型,克隆rh.45
<400>41
atggctgccg atggttatct tccagattgg ctcgaggaca acctctctga gggcattcgc 60
gagtggtggg acctgaaacc tggagccccg aaacccaaag ccaaccagca aaagcaggac 120
gacggccggg gtctggtgct tcctggctac aagtacctcg gacccttcaa cggactcgac 180
aagggggagc ccgtcaacgc ggcggacgca gcggccctcg agcacgacaa ggcctacgac 240
cagcagctca aagcgggtga caatccgtac ctgcggtata accacgccga cgccgagttt 300
caggagcgtc tgcaagaaga tacgtctttt gggggcaacc tcgggcgagc agtcttccag 360
gccaagaagc gggttctcga acctctcggt ctggttgagg aagctgctaa gacggctcct 420
ggaaagaaga gaccggtaga accgtcacct cagcgttccc ccgactcctc cacgggcatc 480
ggcaagaaag gccagcagcc cgctagaaag agactgaact ttgggcagac tggcgactca 540
gagtcagtcc ccgaccctca accaatcgga gaaccaccag caggcccctc tggtctggga 600
tctggtacaa tggctgcagg cggtggcgct ccaatggcag acaataacga aggcgccgac 660
ggagtgggta atgcctcagg aaattggcat tgcgattcca catggctggg cgacagagtc 720
atcaccacca gcacccgaac ctgggctttg cccacctaca acaaccacct ctacaagcaa 780
atctccagtc agtcagcagg tagcaccaac gacaacgtct acttcggcta cagcaccccc 840
tgggggtatt ttgacttcaa cagattccac tgtcacttct caccacgtga ctggcagcgg 900
ctcatcaaca acaactgggg attccggccc aagaagctca acttcaagct gttcaacatc 960
caggtcaagg aggtcacaac gaatgacggc gtcacgacca tcgccaataa ccttaccagc 1020
acggttcagg tcttttcgga ctcggaatac cagctgccct acgtcctcgg ctccgcacac 1080
cagggctgcc tgcctccgtt cccggcggac gtcttcatga ttcctcagta cggctacctg 1140
actctcaaca acggtagtca ggccgtggga cgttcctcct tctactgcct ggagtacttc 1200
ccctctcaga tgctgagaac gggcaacaac ttttccttca gctacacttt cgaggacgtg 1260
cctttccaca gcagctacgc gcacagccag agtttggaca ggctgatgaa tcctctcatc 1320
gaccagtacc tgtactacct gtcaagaacc cagtctacgg gaggcacagc gggaacccag 1380
cagttgctgt tttctcaggc cgggcctagc aacatgtcga ctcaggccag aaactggctg 1440
cctggaccct gctacagaca gcagcgcgtc tccacgacac tgtcgcaaaa caacaacagc 1500
aactttgcct ggactggtgc caccaagtat catctgaacg gcagagactc tctggtgaat 1560
ccgggcgtcg ccatggcaac caacaaggac gacgaggacc gcttcttccc atccagcggc 1620
atcctcatgt ttggcaagca gggagctgga aaagacaacg tggactatag caacgtgatg 1680
ctaaccagcg aggaagaaat caagaccacc aaccccgtgg ccacagaaca gtatggcgtg 1740
gtggctgata acctacagca gcaaaacacc gctcctattg tgggggccgt caacagccag 1800
ggagccttac ctggcatggt ctggcagaac cgggacgtgt acctgcaggg tcctatttgg 1860
gccaagattc ctcacacaga tggcaacttt cacccgtctc ctttaatggg cggctttgga 1920
cttaaacatc cgcctcctca gatccttatc aaaaacactc ctgttcctgc ggatcctcca 1980
acagcgttca accaggccaa gctgaattct ttcatcacgc agtacagcac cggacaagtc 2040
agcgtggaga tcgagtggga gctgcagaag gagaacagca agcgctggaa cccagagatt 2100
cagtatactt ccaactacta caaatctaca aatgtggact ttgctgttaa tactgagggt 2160
gcttactctg agcctcgccc cattggcact cgttacctca cccgtaatct gtaa 2214
<210>42
<211>2217
<212>DNA
<213>新AAV血清型,克隆rh.59
<400>42
atggctgctg acggttatct tccagattgg ctcgaggaca acctctctga gggcattcgc 60
gagtggtggg acctgaaacc tggagccccc aagcccaagg ccaaccagca gaagcaggac 120
gacggccggg gtctggtgct tcctggctac aagtacctcg gacccttcaa cggactcgac 180
aagggggagc ccgtcaacgc ggcggacgca gcggccctcg agcacgacaa ggcctacgac 240
cagcagctca aagcgggtga caatccgtac ctgcggtata accacgccga cgccgagttt 300
caggagcgtc tgcaagaaga tacgtctttt gggggcaacc tcgggcgagc agtcttccag 360
gccaagaagc gggttctcga acctctcggt ctggttgagg aagctgctaa gacggctcct 420
ggaaagaaga gaccggtaga accgtcacct cagcgttccc ccgactcctc cacgggcatc 480
ggcaagaaag gccagcagcc cgctagaaag agactgaact ttgggcagac tggcgactca 540
gagtcagtcc ccgaccctca accaatcgga gaaccaccag caggcccctc tggtctggga 600
tctggtacaa tggctgcagg cggtggcgca ccaatggctg acaataacga gggcgccgac 660
ggagtgggta atgcctcagg aaattggcat tgcgattcca catggctggg cgacagagtc 720
atcaccacca gcacccgaac ctgggctttg cccacctaca acaaccacct ctacaagcaa 780
atctccagtc agtcagcagg tagcaccaac gacaacgtct acttcggcta cagcaccccc 840
tgggggtatt ttgacttcaa cagattccac tgtcacttct caccacgtga ctggcagcgg 900
ctcatcaaca acaactgggg attccggccc aagaagctca acttcaagct gttcaacatc 960
caggtcaagg aggtcacaac gaatgacggc gtcacgacca tcgccaataa ccctaccagc 1020
acggttcagg tcttttcgga ctcggaatac cagctgccct acgtcctcgg ctccgcacac 1080
cagggctgcc tgcctccgtt cccggcggac gtcttcatga ttccccagta cggctacctg 1140
actctgaaca atggcagcca atcggtgggt cgttcctctt tctactgcct ggaatatttc 1200
ccttctcaaa tgctgagaac gggcaacaac ttcaccttca gctacacctt cgaggacgtt 1260
cccttccaca gcagctacgc acacagccag agcctggacc ggctgatgaa tcctcttatc 1320
gaccagtacc tgtattacct ggccagaaca cagagcaacg caggaggcac agctggcaat 1380
cgggaactgc agttttatca gggcgggcct accaccatgg ccgaacaagc caaaaactgg 1440
ctgcctggac cctgctacag acagcagcgc gtctccacga cactgtcgca aaacaacaac 1500
agcaactttg cctggactgg tgccaccaag tatcatctga acggcagaga ctctctggtg 1560
aatccgggcg tcgccatggc aaccaacaag gacgacgagg accgcttctt cccatccagc 1620
ggcatcctca tgtttggcaa gcagggagct ggaaaagaca acgtggacta tagcaacgtg 1680
atgctaacca gcgaggaaga aatcaagacc accaaccccg tggccacaga acagtatggc 1740
gtggtggctg ataacctaca gcagcaaaac accgctccta ttgtgggggc cgtcaacagc 1800
cagggagcct tacctggcat ggtctggcag aaccgggacg tgtacctgca gggtcctatt 1860
tgggccaaga ttcctcacac agatggcaac tttcacccgt ctcctttaat gggcggcttt 1920
ggacttaaac atccgcctcc tcagatcctc atcaaaaaca ctcctgttcc tgcggatcct 1980
ccaacagcgt tcaaccaggc caagctgaat tctttcatca cgcagtacag caccggacaa 2040
gtcagcgtgg agatcgagtg ggagctgcag aaggagaaca gcaagcgctg gaacccagag 2100
attcagtata cttccaacta ctacaaatct acaaatgtgg actttgctgt taatactgag 2160
ggtgtttact ctgagcctcg ccccattggc actcgttacc tcacccgtaa tctgtaa 2217
<210>43
<211>2211
<212>DNA
<213>新AAV血清型,克隆rh.43
<400>43
atggctgccg atggttatct tccagattgg ctcgaggaca acctctctga gggcattcgc 60
gagtggtggg acttgaaacc tggagccccg aaacccaaag ccaaccagca aaagcaggac 120
gacggccggg gcctggtgct tcctggctac aagtacctcg gacccttcaa cggactcgac 180
aagggggagc ccgtcaacgc ggcggacgca gcggccctcg agcacgacaa ggcctacgac 240
cagcagctcg aagcgggtga caatccgtac ctgcggtata accacgccga cgccgagttt 300
caggagcgtc tgcaagaaga tacgtctttt gggggcaacc tcgggcgagc agtcttccag 360
gccaagaagc gggttctcga acctctcggt ctggttgagg aaggcgctaa gacggctcct 420
ggaaagaaga gaccagtaga gcagtcaccc caagaaccag actcctcctc gggcatcggc 480
aagaaaggcc aacagcccgc cagaaaaaga ctcaattttg gccagactgg cgactcagag 540
tcagttccag accctcaacc tctcggagaa cctccagcag cgccctctgg tgtgggacct 600
aatacaatgg ctgcaggcgg tggcgcacca atggcagaca ataacgaagg cgccgacgga 660
gtgggtagtt cctcgggaaa ttggcattgc gattccacat ggctgggcga cagagtcatc 720
accaccagca cccgaacctg ggccctgccc acctacaaca accacctcta caagcaaatc 780
tccaacggga catcgggagg agccaccaac gacaacacct acttcggcta cagcaccccc 840
tgggggtatt ttgactttaa cagattccac tgccactttt caccacgtga ctggcagcga 900
ctcatcaaca acaactgggg attccggccc aagagactca gcttcaagct cttcaacatc 960
caggtcaagg aggtcacgca gaatgaaggc accaagacca tcgccaataa cctcaccagc 1020
accatccagg tgtttacgga ctcggagtac cagctgccgt acgttctcgg ctctgcccac 1080
cagggctgcc tgcctccgtt cccggcggac gtgttcatga ttccccagta cggctaccta 1140
acactcaaca acggtagtca ggccgtggga cgctcctcct tctactgcct ggaatacttt 1200
ccttcgcaga tgctgagaac cggcaacaac ttccagttta cttacacctt cgaggacgtg 1260
cctttccaca gcagctacgc ccacagccag agcttggacc ggctgatgaa tcctctgatt 1320
gaccagtacc tgtactactt gtctcggact caaacaacag gaggcacggc aaatacgcag 1380
actctgggct tcagccaagg tgggcctaat acaatggcca atcaggcaaa gaactggctg 1440
ccaggaccct gttaccgcca acaacgcgtc tcaacgacaa ccgggcaaaa caacaatagc 1500
aactttgcct ggactgctgg gaccaaatac catctgaatg gaagaaattc attggctaat 1560
cctggcatcg ctatggcaac acacaaagac gacgaggagc gttttttccc agtaacggga 1620
tcctgttttt ggcaacaaaa tgctgccaga gacaatgcgg attacagcga tgtcatgctc 1680
accagcgagg aagaaatcaa aaccactaac cctgtggcta cagaggaata cggtatcgtg 1740
gcagataact tgcagcagca aaacacggct cctcaaattg gaactgtcaa cagccagggg 1800
gccttacccg gtatggtctg gcagaaccgg gacgtgtacc tgcagggtcc catctgggcc 1860
aagattcctc acacggacgg caacttccac ccgtctccgc tgatgggcgg ctttggcctg 1920
aaacatcctc cgcctcagat cctgatcaag aacacgcctg tacctgcgga tcctccgacc 1980
accttcaacc agtcaaagct gaactctttc atcacgcaat acagcaccgg acaggtcagc 2040
gtggaaattg aatgggagct acagaaggaa aacagcaagc gctggaaccc cgagatccag 2100
tacacctcca actactacaa atctacaagt gtggactttg ctgttaatac agaaggcgtg 2160
tactctgaac cccgccccat tggcacccgt tacctcaccc gtaatctgta a 2211
<210>44
<211>2211
<212>DNA
<213>新AAV血清型,克隆hu.3
<400>44
atggctgccg atggttatct tccagattgg ctcgaggaca ctctctctga aggaataaga 60
cagtggtgga agctcaaacc tggcccacca ccaccaaagc ccgcagagcg gcataaggac 120
gacagcaggg gtcttgtgct tcctgggtac aagtacctcg gacccttcaa cggactcgac 180
aagggggagc cggtcaacga ggcagacgcc gcggccctcg agcacgacaa ggcctacgac 240
cggcagctcg acagcggaga caacccgtac ctcaagtaca accacgccga cgcagagttt 300
caggagcgcc ttaaagaaga tacgtctttt gggggcaacc tcggacgagc agtcttccag 360
gcgaaaaaga gggttcttga acctctgcgg cctggtttga ggaaacctgt taagacggct 420
ccgggaaaaa agaggccggt agagcactct cctgtggagc cagactcctc ctcgggaacc 480
ggaaaagcgg gccagcagcc tgcaagaaaa agattaaatt ttggtcagac tggagacgca 540
gactccgtac ctgaccccca gcctctcgga cagccaccag cagccccctc tggtctggga 600
tctactacaa tggctacagg cagtggcgca ccaatggcag acaataacga gggtgccgat 660
ggagtgggta attcctcagg aaattggcat tgcgattccc aatggctgga cgacagagtc 720
atcgccacca gcacccgaac ctgggccctg cccacataca acaaccacct ctacaagcaa 780
atctccagcc aatcaggagc ctgcaacgac aaccactact ttggctacag caccccctgg 840
gggtattttg acttcaacag attccactgc cacttttcac cacgtgactg gcaaagactc 900
atcaacagca actggggatt ccggcccaaa agactcaact tcaagctctt taatattcaa 960
gtcaaagagg tcacgcagaa tgacggtacg acgacgattg ccaataacct taccagcacg 1020
gttcaggtgt ttactgactc ggagtaccag ctcccgtacg tcccgggctc ggcgcatcaa 1080
ggatgcctcc cgccgtttcc agcggacgtc ttcatggtcc cacagtatgg atacctcacc 1140
ctgaacaacg ggagtcaggc ggtaggacgc tcttcctttt actgcctgga gtactttcct 1200
tctcagatgc tgcgtactgg aaacaacttt cagttcagct acacttttga agacgtgcct 1260
ttccacagca gctacgctca ctgccagagt ctggatcggc tgatgaatcc tctgatcgac 1320
cagtacctgt attatctgaa caagacacaa acaaatagtg gaactcttca gcagtctcgg 1380
ctactgttta gccaagctgg accaaccaac atgtctcttc aagctaaaaa ctggctgcct 1440
ggaccttgct acagacagca gcgtctgtca aaacaggcaa acgacaataa caactgcaac 1500
tttccctgga ctgcagctac aaagtatcat ctaaatggcc gggactcgtt ggttaatcca 1560
ggaccagcta tggccagtca caaggatgac gaagaaaagt ttttccccat gcatggaacc 1620
ctgatatttg gtaaacaagg aacaaatgcc aacgacgcgg atttggaaaa tgtcatgatt 1680
acagatgaag aagaaatcag gcccaccaat cccgtggcta cggagcagta cgggactgtg 1740
tcaaataatt tgcaaaactc aaacactggt ccaactacag gaactgtcaa tcaccaagga 1800
gcgttacctg gtatggtgtg gcaggatcga gacgtgtacc tgcagggacc catttgggcc 1860
aagattcctc acaccgatgg acactttcat ccttctccac tgatgggagg ttttggactc 1920
aaacacccgc ctcctcagat catgatcaaa agcactcccg ttccagccaa tcctcccaca 1980
aacttcagtt ctgccaagtt tgcttcttcc atcacacagt attccacggg acaggtcagc 2040
gtggagatcg agtgggagct gcagaaggag aacagcaaac gctggaatcc cgaaattcag 2100
tacacttcca actacaacaa gtctgttaat gtggacttta ctgtggacac taatggtgtg 2160
tattcagagc ctcgccccat tggcaccaga tacctgactc gtaatctgta a 2211
<210>45
<211>2208
<212>DNA
<213>新AAV血清型,克隆hu.5
<400>45
atggctgccg atggttatct tccagattgg ctcgaggaca ctctctctga aggaataaga 60
cagtggtgga agctcaaacc tggcccacca ccaccaaagc ccgcagagcg gcataaggac 120
gacagcaggg gtcttgtgct tcctgggtac aagtacctcg gacccttcaa cggactcgac 180
aagggggagc cggtcaacga ggcagacgcc gcggccctcg agcacgacaa ggcctacgac 240
cggcagctcg acagcggaga caacccgtac ctcaagtaca accacgccga cgcagagttt 300
caggagcgcc ttaaagaaga tacgtctttt gggggcaacc tcggacgagc agtcttccag 360
gcgaaaaaga gggttcttga acctctgggc ctggttgagg aacctgttaa gacggctccg 420
ggaaaaaaga ggccggtaga gcactctcct gtggagccag actcctcctc gggaaccgga 480
aaagcgggcc agcagcctgc aagaaaaaga ttaaattttg gtcagactgg agacgcagac 540
tccgtacctg acccccagcc tctcggacag ccaccagcag ccccctctgg tctgggatct 600
actacaatgg ctacaggcag tggcgcacca atggcagaca ataacgaggg tgccgatgga 660
gtgggtaatt cctcaggaaa ttggcattgc gattcccaat ggctgggcga cagagtcatc 720
accaccagca cccgaacctg ggccctgccc acatacaaca accacctcta caagcaaatc 780
tccagccaat caggagccag caacgacaac cactactttg gctacagcac cccctggggg 840
tattttgact tcaacagatt ccactgccac ttttcaccac gtgactggca aagactcatc 900
aacaacaact ggggattccg gcccaaaaga ctcaacttca agctctttaa tattcaagtc 960
aaagaggtca cgcagaatga cggtacgacg acgattgcca ataaccttac cagcacggtt 1020
caggtgttta ctgactcgga gtaccagctc ccgtacgtcc tgggctcggc gcatcaagga 1080
tgcctcccgc cgtttccagc ggacgtcttc atggtcccac agtatggata cctcaccctg 1140
aacaacggga gtcaggcggt aggacgctct tccttttact gcctggagta ctttccttct 1200
cagatgctgc gtactggaaa caactttcag ttcagctaca cttttgaaga cgtgcctttc 1260
cacagcagct acgctcacag ccagagtctg gatcggctga tgaatcctct gatcgaccag 1320
tacctgtatt atctgaacaa gacacaaaca aatagtggaa ctcttcagca gtctcggcta 1380
ctgtttagcc aagctggacc aaccaacatg tctcttcaag ctaaaaactg gctgcctgga 1440
ccttgctaca gacagcagcg tctgtcaaaa caggcaaacg acaacaacaa cagcaacttt 1500
ccctggactg cagctacaaa gtatcatcta aatggccggg actcgttggt taatccagga 1560
ccagctatgg ccagtcgcaa ggatgacgaa gaaaagtttt tccccatgca tggaaccctg 1620
atatttggta aacaaggaac aaatgccaac gacgcggatt tggaaaatgt catgattaca 1680
gatgaagaag aaatcagggc caccaatccc gtggctacgg agcagtacgg gactgtgtca 1740
aataatttgc aaaactcaaa cactggtcca actactggaa ctgtcaatca ccaaggagcg 1800
ttacctggta tggtgtggca ggatcgagac gtgtacctgc agggacccat ttgggccaag 1860
attcctcaca ccgatggaca ctttcatcct tctccactga tgggaggttt tggactcaaa 1920
cacccgcctc ctcagatcat gatcaaaaac actcccgttc cagccaatcc tcccacaaac 1980
ttcagttctg ccaagtttgc ttctttcatc acacagtatt ccacgggaca ggtcagcgtg 2040
gagatcgagt gggagctgca gaaggagaac agcaaacgct ggaatcccga aattcagtac 2100
acttccaact acaacaagtc tgttaatgtg gactttactg tggacactaa tggtgtgtat 2160
tcagagcctc gccccattgg caccagatac ctgactcgta atctgtaa 2208
<210>46
<211>2208
<212>DNA
<213>新AAV血清型,克隆hu.1
<400>46
atggctgccg atggttatct tccagattgg ctcgaggaca ctctctctga aggaataaga 60
cagtggtgga agctcaaacc tggcccacca ccaccaaagc ccgcagagcg gcataaggac 120
gacagcaggg gtcttgtgct tcctgggtac aagtacctcg gacccttcaa cggactcgac 180
aagggggagc cggtcaacga ggcagacgcc gcggccctcg agcacgacaa ggcctacgac 240
cggcagctcg acagcggaga caacccgtac ctcaagtaca accacgccga cgcagagttt 300
caggagcgcc ttaaagaaga tacgtctttt gggggcaacc tcggacgagc agtcttccag 360
gcgaaaaaga gggttcttga acctctgggc ctggttgagg aacctgttaa gacggctccg 420
ggaaaaaaga ggccggtaga gcactctcct gtggagccag actcctcctc gggaaccgga 480
aaagcgggcc agcagcctgc aagaaaaaga ttaaattttg gtcagactgg agacgcagac 540
tccgtacctg acccccagcc tctcggacag ccaccagcag ccccctctgg tctgggatct 600
actacaatgg ctacaggcag tggcgcacca atggcagaca ataacgaggg tgccgatgga 660
gtgggtaatt cctcaggaaa ttggcattgc gattcccaat ggctgggcga cagagtcatc 720
accaccagca cccgaacctg ggccctgccc acatacaaca accacctcta caagcaaatc 780
tccagccaat caggagccag caacgacaac cactactttg gctacagcac cccctggggg 840
tattttgact tcaacagatt ccactgccac ttttcaccac gtgactggca aagactcatc 900
aacaacaact ggggattccg gcccaaaaga ctcaacttca agctctttaa tattcaagtc 960
aaagaggtca cgcagaatgg cggtacgacg acgattgcca ataaccttac cagcacggtt 1020
caggtgttta ctgactcgga gtaccagctc ccgtacgtcc tgggctcggc gcatcaagga 1080
tgcctcccgc cgtttccagc ggacgtcttc atggtcccac agtatggata cctcaccctg 1140
aacaacggga gtcaggcggt aggacgctct tccttttact gcctggagta ctttccttct 1200
cagatgctgc gtactggaaa caactttcag ttcagctaca cttttgaaga cgtgcctttc 1260
cacagcagct acgctcacag ccagagtctg gatcggctga tgaatcctct gatcgaccag 1320
tacctgtatt atctgaacaa gacacaaaca aatagtggaa ctcttcagca gtctcggcta 1380
ctgtttagcc aagctggacc aaccaacatg tctcttcaag ctaaaaactg gctgcctgga 1440
ccttgctaca gacagcagcg tctgtcaaaa caggcaaacg gcaacaacaa cagcaacttt 1500
ccctggactg cagctacaaa gtatcatcta aatggccggg actcgttggt taatccagga 1560
ccagctatgg ccagtcacaa ggatgacgaa gaaaagtttt tccccatgca tggaaccctg 1620
atatttggta aacaaggaac aaatgccaac gacgcggatt tggaaaatgt catgattaca 1680
gatgaagaag aaatcagggc caccaatccc gtggctacgg agcagtacgg gactgtgtca 1740
aataatttgc aaaactcaaa cactggtcca actactggaa ctgtcaatca ccaaggagcg 1800
ttacctggta tggtgtggca ggatcgagac gtgtacctgc agggacccat ttgggccaag 1860
attcctcaca ccgatggaca ctttcatcct tctccactga cgggaggttt tggactcaaa 1920
cacccgcctc ctcagatcat gatcaaaaac actcccgttc cagccaatcc tcccacaaac 1980
ttcagttctg ccaagtttgc ttctttcatc acacagtatt ccacgggaca ggtcagcgtg 2040
gagatcgagt gggagctgca gaaggagaac agcaaacgct ggaatcccga aattcagtac 2100
acttccaact acaacaagtc tgttaatgtg gactttactg tggacactaa tggtgtgtat 2160
tcagagcctc gccccattgg caccagatac ctgactcgta atctgtaa 2208
<210>47
<211>2208
<212>DNA
<213>新AAV血清型,克隆hu.4
<400>47
atggctgccg atggttatct tccagattgg ctcgaggaca ctctctctga aggaataaga 60
cagtggtgga agctcaaacc tggcccacca ccaccaaagc ccgcagagcg gcataaggac 120
gacagcaggg gtcttgtgct tcctgggtac aagtacctcg gacccttcaa cggactcgac 180
aagggggagc cggtcaacga ggcagacgcc gcggccctcg agcacgacaa ggcctacgac 240
cggcagctcg acagcggaga caacccgtac ctcaagtaca accacgccga cgcagagttt 300
caggagcgcc ttaaagaaga tacgtctttt gggggcaacc tcggacgagc agtcttccag 360
gcgaaaaaga gggttcttga acctctgggc ctggttgagg agcctgttaa gacggctccg 420
ggaaaaaaga ggccggtaga gcactctcct gtggagccag actcctcctc gggaaccgga 480
aaagcgggcc agcagcctgc aagaaaaaga ttaaattttg gtcagactgg agacgcagac 540
tccgtacctg acccccagcc tctcggacag ccaccagcag ccccctctgg tctgggatct 600
actacaatgg ctacaggcag tggcgcacca atggcagaca ataacgaggg tgccgatgga 660
gtgggtaatt cctcaggaaa ttggcattgc gattcccaat ggctgggcga cagagtcatc 720
accaccagca cccgaacctg ggccctgccc acatacaaca accacctcta caagcaaatc 780
tccagccaat caggagccag caacgacaac cactactttg gctacagcac cccctggggg 840
tattttgact tcaacagatt ccactgccac ttttcaccac gtgactggca aagactcgtc 900
aacaacaacc ggggattccg gcccaaaaga ctcaacttca agctctttaa tattcaagtc 960
aaagaggtca cgcagaatga cggtacgacg acgattgcca ataaccttac cagcacggtt 1020
caggtgttta ctgactcgga gtaccagctc ccgtacgtcc tgggctcggc gcatcaagga 1080
tgcctcccgc cgtttccagc ggacgtcttc atggtcccac agtatggata cctcaccctg 1140
aacaacggga gtcaggcggt aggacgctct tccttttact gcctggagta ctttccttct 1200
cagatgctgc gtactggaaa caactttcag ttcagctaca cttttgaaga cgtgcctttc 1260
cacagcagct acgctcacag ccagagtctg gatcggctga tgaatcctct gatcgaccag 1320
tacctgtatt atctgaacaa gacacaaaca aatagtggaa ctcttcagca gtctcggcta 1380
ctgtttagcc aagctggacc aaccaacatg tctcttcaag ctaaaaactg gctgcctgga 1440
ccttgctaca gacagcagcg tctgtcaaaa caggcaaacg acaacaacaa cagcaacttt 1500
ccctggactg cagctacaaa gtatcatcta aatggccggg actcgttggt taatccagga 1560
ccagctatgg ccagtcacaa ggatgacgaa gaaaagtttt tccccatgca tggaaccctg 1620
atatttggta aacaaggaac aaatgccaac gacgcggatt tggaaaatgt catgattaca 1680
gatgaagaag aaatcagggc caccaatccc gtggctacgg agcagtacgg gactgtgtca 1740
aataatttgc aaaactcaaa cactggtcca actactggaa ctgtcaatca ccaaggagcg 1800
ttacctggta tggtgtggca ggatcgagac gtgtacctgc agggacccat ttgggccaag 1860
attcctcaca ccgatggaca ctttcatcct tctccactga tgggaggttt tggactcaaa 1920
cacccgcctc ctcagatcat gatcaaaaac actcccgttc cagccaatcc tcccacaaac 1980
ttcagttctg ccaagtttgc ttctttcatc acacagtatt ccacgggaca ggtcagcgtg 2040
gagatcgagt gggagctgca gaaggagaac agcaaacgct ggaatcccga aattcagtac 2100
acttccaact acaacaagtc tgttaatgtg gactttactg tggacactaa tggtgtgtat 2160
tcagagcctc gccccattgg caccagatac ctgactcgta atctgtaa 2208
<210>48
<211>2209
<212>DNA
<213>新AAV血清型,克隆hu.2
<400>48
atggctgccg atggttatcc tccagattgg ctcgaggaca ctctctctga agggataaga 60
cagtggtgga agctcaaacc tggcccacca ccaccaaagc ccgcagagcg gcataaggac 120
gacagcaggg gtcttgtgct tcctgggtac aagtacctcg gacccttcaa cggactcgac 180
aagggggagc cggtcaacga ggcagacgcc gcggccctcg agcacgacaa ggcctacgac 240
cggcagctcg acagcggaga caacccgtac ctcaagtaca accacgccga cgcagagttt 300
caggagcgcc ttaaagaaga tacgtctttt gggggcaacc tcggacgagc agtcttccag 360
gcgaaaaaga gggttcttga acctctgggc ctggttgagg aacctgttaa gacggctccg 420
ggaaaaaaga ggccggtaga gcactctcct gtggagccag actcctcctc gggaaccgga 480
aaagcgggcc agcggcctgc aagaaaaaga ttaaattttg gtcagactgg agacgcagac 540
tccgtacctg acccccagcc tctcggacag ccaccagcag ccccctctgg tctgggatct 600
actacaatgg ctacaggcag tggcgcacca atggcagaca ataacgaggg tgccgatgga 660
gtgggtaatt cctcaggaaa ttggcattgc gattcccaat ggctgggcga cagagtcatc 720
accaccagca cccgaacctg ggccctgccc acatacaaca accacctcta caagcaaatc 780
tccagccaat caggagccag caacgacaac cactactttg gctacagcac cccctggggg 840
tattttgact tcaacagatt ccactgccac ttttcaccac gtgactggca aagactcatc 900
aacaacaact ggggattccg gcccaaaaga ctcaacttca agctctttaa tattcaagtc 960
aaagaggtca cgcagaatga cggtacgacg acgattgcca ataaccttac cagcacggtt 1020
caggtgttta ctgactcgga gtaccagctc ccgtacgtcc tgggctcggc gcatcaagga 1080
tgcctcccgc cgtttccagc ggacgtcttc atggtcccac agtatggata cctcaccctg 1140
aacaacggga gtcaggcggt aggacgctct tccttttact gcctggagta ctttccttct 1200
cagatgctgc gtactggaaa caactttcag ttcagctaca cttttgaaga cgtgcctttc 1260
cacagcagct acgctcacag ccagagtctg gatcggctga tgaatcctct gatcgaccag 1320
tacctgtatt atctgaacaa gacacaaaca aatagtggaa ctcttcagca gtctcggcta 1380
ctgtttagcc aagctggacc aaccaacatg tctcttcaag ctaaaaactg gctgcctgga 1440
ccttgctaca gacagcagcg tctgtcaaaa caggcaaacg acaacaacaa cagcaacttt 1500
ccctggactg cagctacaaa gtatcatcta aatggccggg actcgttggt taatccagga 1560
ccagctatgg ccagtcacaa ggatgacgaa gaaaagtttt tccccatgca tggaaccctg 1620
atatttggta aacaaggaac aaatgccaac gacgcggatt tggaaaatgt catgattaca 1680
gatgaagaag aaatcagggc caccaatccc gtggctacgg agcagtacgg gactgtgtca 1740
aataatttgc aaaactcaaa cactggtcca actactggaa ctgtcaatcg ccaaggagcg 1800
ttacctggta tggtgtggca ggatcgagac gtgtacctgc agggacccat ttgggccaag 1860
attcctcaca ccgatggaca ctttcatcct tctccactga tgggaggttt tggactcaaa 1920
cacccgcctc ctcagatcat gatcaaaaac actcccgttc cagccaatcc tcccacaaac 1980
ttcagttctg ccaagtttgc ttctttcatc acacagtatt ccacgggaca ggtcagcgtg 2040
gagatcgagt gggagctgca gaaggagaac agcaaacgct ggaatcccga aattcagtac 2100
acttccaact acaacaagtc tgttaatgtg gactttactg tggacactaa tggtgtgtat 2160
tcagagcctc gccccattgg caccagatac ctgactcgta atctgtaat 2209
<210>49
<211>2208
<212>DNA
<213>新AAV血清型,克隆hu.25
<400>49
atggctgccg atggttatct tccagattgg ctcgaggaca ctctctctga aggaataaga 60
cagtggtgga agctcaaacc tggcccacca ccaccaaagc ccgcagagcg gcataaggac 120
ggcagcaggg gtcttgtgct tcctgggtac aagtacctcg gacccttcaa cggactcgac 180
aagggagagc cggtcaacga ggcagacgcc gcggccctcg agcacgacaa ggcctacgac 240
cggcagctca acagcggaga caacccgtac ctcaagtaca accacgccga cgcggagttt 300
caggagcgcc ttaaagaaga tacgtctttt gggggcaacc tcggacgagc agtcttccag 360
gcaaaaaaga gggttcttga acctctgggc ctggttgagg agcctgttaa aacggctccg 420
ggaaaaaaga gaccggtaga gcactctcct gcggagccag actcctcctc gggaaccgga 480
aaagcgggcc agcagcctgc aagaaagaga ttgaattttg gtcagactgg agacgcagac 540
tccgtacctg acccccagcc tctcggacag ccaccagcag ccccctctgg tctgggatct 600
actacaatgg ctacaggcag tggcgcacca atggcagaca ataacgaggg tgccgatgga 660
gtgggtaatt cctcaggaaa ttggcattgc gattcccaat ggctgggcga cagagtcatc 720
accacaagca ctcgaacctg ggccctgccc acctacaaca accacctcta caagcaaatc 780
tccagccaat caggagcctc aaacgacaac cactattttg gctacagcac cccttggggg 840
tattttgact tcaacagatt ccactgccac ttttcaccac gtgactggca aagactcatc 900
aacaacaact ggggattccg acccaagaga ctcaacttca agctctttaa cattcaagtc 960
aaagaggtca cgcagaatga cggtacgacg acgattgcca ataaccttac cagcacggtt 1020
caggtgttta ctgactcgga gtaccagctc ccgtacgtcc tcggctcggc gcatcaagga 1080
tgcctcccgc cgttcccagc ggacgtcttc atggtcccac agtatggata cctcaccctg 1140
aacaacggga gtcaggcggt aggacgctct cccttttact gcctggagta ctttccttct 1200
cagatgctgc gtactggaaa caactttcag ttcagctaca cttttgaaga cgtgcctttc 1260
cacagcagct acgctcacag ccagagtctg gatcggctga tgaatcctct gatcgaccag 1320
tacctgtatt atctgaacaa gacacaaaca aatagtggaa ctcttcagca gtctcggcta 1380
ctgtttagcc aagctggacc caccaacatg tctcttcaag ctaaaaactg gctgcctgga 1440
ccttgctaca gacagcagcg tctgtcaaag caggcaaacg acaacaacaa cagcaacttt 1500
ccctggactg cagctacaaa gtatcatcta aatggccggg actcgttggt taatccagga 1560
ccagctatgg ccagtcacaa ggatgacgaa gaaaagtttt tccccatgca tggaaccctg 1620
atatttggta aacaaggaac aaatgccaac gacgcggatt tggaaaatgt catgattaca 1680
gatgaagaag aaatcaggac caccaatccc gtggctacgg agcagtacgg gactgtgtca 1740
aataatttgc aaaactcaaa cactggtcca actactggaa ctgtcaatca ccaaggagcg 1800
ttacctggta tggtgtggca ggatcgagat gtgtaccttc agggacccat ttgggccaag 1860
attcctcaca ccgatggaca ctttcatcct tctccactga tgggaggttt tggactcaaa 1920
cacccgcctc ctcagattat gatcaaaaac actcccgttc cagccaatcc tcctacaaac 1980
ttcagttctg ccaagtttgc ttctttcatc acacagtatt ccacggggca ggtcagcgtg 2040
gagatcgagt gggagctgca gaaggagaac agcaaacgct ggaatcccga aattcagtac 2100
acttccaact acaacaaatc tgttaatgtg gactttactg tggacaataa tggcgtgtac 2160
tcagagcctc gccccattgg caccagatac ctgactcgta atctgtaa 2208
<210>50
<211>2208
<212>DNA
<213>新AAV血清型,克隆hu.15
<400>50
atggctgccg atggttatct tccagattgg ctcgaggaca ctctctctga aggaataaga 60
cagtggtgga agctcaaacc tggcccacca ccaccaaagc ccgcagagcg gcataaggac 120
gacagcaggg gtcttgtgct tcctgggtac aagtacctcg gacccttcaa cggactctac 180
aagggagagc cggtcgacga ggcagacgcc gcggccctcg agcacgacaa ggcctacgac 240
cggcagctcg acagcggaga caacccgtac ctcaagtaca accacgccga cgcggagttt 300
caggagcgcc ttaaagaaga tacgtctttt gggggcaacc tcggacgagc agtcttccag 360
gccaaaaaga gggttcttga acctctgggc ttggttgggg agcctgttaa aacggctccg 420
ggaaaaaaga ggccggtaga gcactctcct gtggagccag actcctcctc gggaaccgga 480
aaagcgggca accagcctgc aagaaaaaga ttgaattttg gtcagactgg agacgcagac 540
tccgtacctg acccccagcc tctcggacag ccaccagcag ccccctctgg tctgggatct 600
actacaatgg ctacaggcag tggcgcacca gtggcagaca ataacgaggg tgccgatgga 660
gtgggtaatt cctcaggaaa ttggcattgc gattcccaat ggctgggcga cagagtcatc 720
accaccagca cccgaacctg ggctctgccc acctacaata accacctcta caagcaaatc 780
tccagccaat caggagcctc aaacgacaac cactactttg gctacagcac cccctggggg 840
tattttgact tcaacagatt ccactgccac ttttcaccac gtgaccggca aagactcatc 900
aacaacaact ggggattccg accaaaaaga ctcaacttca agctctttaa cattcaagtc 960
aaagaggtca cgcagaatga cggtacgacg acgattgcca ataaccttac cagcacggtt 1020
caggtgttta ctgactcggg gtaccagctc ccgtacgtcc tcggcttggc gcatcaagga 1080
tgcctcccgc cgttcccagc agacgtcttc atggtgccac agtatggata cctcaccctg 1140
aacaacggga gtcaggcggt aggacgctct tccttttact gcctggagta ctttccttct 1200
cagatgctgc gtactggaaa caactttcag ttcagctaca cctttgaaga cgttcctttc 1260
cacagcagct acgctcacag ccagagtctg gatcggctga tgaatcctct gatcgaccag 1320
tacctgtatt atctgaacaa gacacaatca aatagtggaa ctcttcagca gtctcggcta 1380
ctgtttagcc aagctggacc caccagcatg tctcttcaag ctaaaaactg gctgcctgga 1440
ccttgctaca gacagcagcg tctgtcaaag caggcaaacg acaacaacaa cagcaacttt 1500
ccctggactg cggctacaaa gtatcatcta aatggccggg actcgttggt taatccagga 1560
ccagctatgg ccagccacaa agacgatgaa gaaaagtttt tccccatgca tggaaccctg 1620
atatttggta aacaaggaac aaatgctaac gacgcggatt tggacaatgt catgattaca 1680
gatgaagaag aaatccgcac caccaatccc gtggctacgg agcagtacgg atatgtgtca 1740
aataatttgc aaaactcaaa tactggtcca actactggaa ctgtcaatca ccaaggagcg 1800
ttacctggta tggtgtggca ggatcgagac gtgtacctgc agggacccat ttgggccaag 1860
attcctcaca ccgatggaca ctttcatcct tctccactta tgggaggttt tggactcaaa 1920
cacccacctc ctcagatcat gattaaaaac actcccgttc cagccaatcc tcccacaaac 1980
ttcagttctg ccaagtttgc ttctttcatc acacagtatt ccacgggaca agtcagcgtg 2040
gagatcgagt gggagctgca gaaggaggac agcaaacgct ggaaccccga gatccagtac 2100
acttccaact ataacaaacc tgttaatgtg gactttactg tggacactaa tggtgtgtat 2160
tcagagcctc gccccattgg caccagatac ctgactcgta atctgtaa 2208
<210>51
<211>2208
<212>DNA
<213>新AAV血清型,克隆hu.16
<400>51
atggctgccg atggttatct tccagattgg ctcgaggaca ctctctctga aggaataaga 60
cagtggtgga agctcaaacc tggcccacca ccgccaaagc ccgcagagcg gcataaggac 120
gacagcaggg gtcttgtgct tcctgggtac aagtacctcg gacccttcaa cggactctac 180
aagggagagc cggtcaacga ggcagacgcc gcggccctcg agcacgacaa ggcctacgac 240
cggcagctcg acagcggaga caacccgtac ctcaagtaca accacgccgg cgcggagttt 300
caggagcgcc ttaaagaaga tacgtctttt gggggcaacc tcggacgagc agtcttccag 360
gccaaaaaga gggttcttga acctctgggc ttggttgagg agcctgttaa aacggctccg 420
ggaaaaaaga ggccggtaga gcactctcct gtggagccag actcctcctc gggaaccgga 480
aaagcgggca accagcctgc aagaaaaaga ttgaattttg gtcagactgg agacgcagac 540
tccgtacctg acccccagcc tctcggacag ccaccagcag ccccctctgg tctgggatct 600
actacaatgg ctacaggcag tggcgcacca gtggcagaca ataacgaggg tgccgatgga 660
gtgggtaatt cctcaggaaa ttggcattgc gattcccaat ggctgggcga cagagtcatc 720
accaccagca cccgaacctg ggctctgccc acctacaaca accacctcta caagcaaatc 780
tccagccaat caggagcctc aaacgacaac cactactttg gctacagcac cccctggggg 840
tattttgact tcaacagatt ccactgccac ttttcaccac gtgactggca aagactcatc 900
aacaacaact ggggattccg accaaagaga ctcaacttca agctctttaa cattcaagtc 960
aaagaggtca cgcagaatga cggtacgacg acgattgcca ataaccttac cagcacggtt 1020
caggtgttta ctgactcgga gtaccagctc ccgtacgtcc tcggctcggc gcatcaagga 1080
tgcctcccgc cgttcccagc agacgtcttc atggtgccac agtatggata cctcaccctg 1140
aacaacggga gtcaggcggt aggacgctct tccttttact gcctggagta ctttccttct 1200
cagatgctgc gtactggaaa caactttcag ttcagctaca cctttgaaga cgttcctttc 1260
cacagcagct acgctcacag ccagagtctg gatcggctga tgaatcctct gatcgaccag 1320
tacctgtatt atctgaacaa gacacaatca aatagtggaa cccttcagca gtctcggcta 1380
ctgtttagcc aagctggacc caccagcatg tctcttcaag ctaaaaactg gctgcctgga 1440
ccttgctaca gacagcagcg tctgtcaaag caggcaaacg acaacaacaa cagcaacttt 1500
ccctggactg cggctacaaa gtatcatcta aatggccggg actcgttggt taatccagga 1560
ccagctatgg ccagccacaa agacgatgaa gaaaagtttt tccccatgca tggaaccctg 1620
atatttggta aacaaggaac aaatgctaac gacgcggatt tggacaatgt catgattaca 1680
gatgaagaag aaatccgcac caccaatccc gtggctacgg agcagtacgg atatgtgtca 1740
aataatttgc aagactcaaa tactggtcca actactggaa ctgtcaatca ccaaggagcg 1800
ttacctggta tggtgtggca ggatcgagac gtgtacctgc agggacccat ttgggccaag 1860
attcctcaca ccgatggaca ctttcatcct tctccactta tgggaggttt tggactcaaa 1920
cacccacctc ctcagatcat gattaaaaac actcccgttc cagccaatcc tcccacaaac 1980
ttcagttctg ccaagtttgc ttctttcatc acacagtatt ccacgggaca agtcagcgta 2040
gagatcgagt gggagctgca gaaggagaac agcaaacgct ggaaccccga gatccagtac 2100
acttccaact ataacaaatc tgttaatgtg gactttactg tggacactaa tggtgtgtat 2160
tcagagcctc gccccattgg caccagatac ctgactcgta atctgtaa 2208
<210>52
<211>2208
<212>DNA
<213>新AAV血清型,克隆hu.18
<400>52
atggctgccg atggttatct tccagattgg ctcgaggaca ctctctctga aggaataaga 60
cagtggtgga agctcaaacc tggcccacca ccaccaaagc ccgcagagcg gcataaggac 120
gacagcaggg gtcttgtgct tcctgggtac aagtacctcg gacccttcaa cggactcgac 180
aagggagagc cggtcaacga ggcagacgcc gcggccctcg agcacgacaa ggcctacgac 240
cggcagctcg aaagcggaga caacccgtac ctcaagtaca accacgccga cgcggagttt 300
caggagcgtc ttaaagaaga tacgtctttt gggggcaacc tcggacgagc agtcttccag 360
gcgaaaaaga gggttcttga acctctgggc ctggttgagg agcctgttaa aacggctccg 420
ggaaaaaaga ggccggtaga gcactctcct gtggagccag actcctcctc gggaaccgga 480
aaagcgggcc agcagcctgc gagaaaaaga ttgaattttg gtcagactgg agacgcagac 540
tccgtacctg acccccagcc tctcggacag ccaccagcag ccccctctgg tctgggatct 600
actacaatgg cttcaggcag tggcgcacca gtggcagaca ataacgaggg tgccgatgga 660
gtgggtaatt cctcaggaaa ttggcattgc gattcccaat ggctgggcga cagagtcatc 720
accaccagca cccgaacctg ggccctgccc acctacaaca accacctcta caagcaaatc 780
tccagccaat caggagcctc aaacgacaac cactactttg gctacagcac cccctggggg 840
tattttgact tcaacagatt ccactgccac ttttcaccac gtgactggca aagactcatc 900
aacaacagct ggggattccg acccaaaaga ctcaacttca agctctttaa cattcaagtc 960
aaagaggtca cgcagaatga cggtacgacg acgattgcca ataaccttac cagcacggtt 1020
caagtgttta ccgactcgga gtaccagctc ccgtacgtcc tcggctcggc gcatcaagga 1080
tgcctcccgc cgttcccagc agacgtcttt atggtcccac agtatggata cctcaccctg 1140
aacaacggga gtcaggcggt aggacgctct tccttttact gcctggagta ctttccttct 1200
cagatgctgc gtactggaaa caactttcag ttcagctaca cctttgaaga cgttcccttc 1260
cacagcagct acgctcacag ccagagtctg gatcggctgc tgaatcctct gatcgaccag 1320
tacctatatt atctgaacaa gacacaatca aatagtggaa ctcttcagca gtctcggcta 1380
ctgtttagcc aagctggacc caccagcatg tctcttcaag ctaaaaactg gctgcctgga 1440
ccttgctaca gacagcagcg tctgtcaaag caggcaaacg acaacaacaa cagcaacttt 1500
ccctggactg cggctacaaa gtatcatcta aatggccggg actcgttggt taatccagga 1560
ccagctatgg ccagccacaa agacgatgaa gaaaagtttt tccccatgca tggaaccctg 1620
atatttggta aacaaggaac aaatgctaac gacgcggatt tggacaatgt catgattaca 1680
gatgaagaag aaatccgcac caccaatccc gtggctacgg agcagtacgg atatgtgtca 1740
aataatttgc aaaactcaaa tactggtcca actactggaa ctgtcaatca ccaaggagcg 1800
ttacctggta tggtgtggca ggatcgagac gtgtacctgc agggacccat ttgggccaag 1860
attcctcaca cggacgggca ctttcatcct tctccactaa tgggaggttt tgggctcaaa 1920
cacccgcctc ctcagatcat gatcaaaaac actcccgttc cagccaatcc tcctacaaac 1980
ttcagttctt ccaagtttgc ttctttcatc acacagtatt ccacggggca ggtcagcgtg 2040
gagatcgagt gggagctgca gaaggagaac agcaaacgct ggaatcccga aattcagtat 2100
acttccaact acaacaaatc tgttaatgtg gactttactg tggacactaa tggtgtgtat 2160
tcagagcctc gccccattgg caccagatac ccgactcgta atctgtaa 2208
<210>53
<211>2208
<212>DNA
<213>新AAV血清型,克隆hu.8
<400>53
atggctgccg atggttatct tccagattgg ctcgaggaca ctctctctga aggaacaaga 60
cagtggtgga agctcaaacc tggcccacca ccaccaaagc ccgcagagcg gcataaggac 120
gacagcaggg gtcttgtgct tcctgggtac aagtacctcg gacccttcaa cggactcgac 180
aagggggagc cggtcaacga ggcagacgcc gcggccctcg agcacgacaa ggcctacgac 240
cggcagctcg acagcggaga caacccgtac ctcaagtaca accacgccga cgcggagttt 300
caggagcgtc ttaaagaaga tacgtctttt gggggcaacc tcggacgagc agtcttccag 360
gcgaaaaaga gggttcttga acctctgggc ctggttgagg aacctgttaa gacggctccg 420
ggaaaaaaga ggccggtaga gcactctcct gcggagccag actcctcctc gggaaccgga 480
aaagcgggcc agcagcctgc aagaaaaaga ttgaattttg gtcagactgg agacgcagac 540
tccgtacctg acccccagcc tctcggacag ccaccagcag ccccctctgg tttgggatct 600
actacaatgg ctacaggcag tggcgcacca atggcagaca ataacgaggg tgccgatgga 660
gtgggtaatt cctcaggaaa ttggcattgc gattcccaat ggctgggcga cagagtcatc 720
accaccagca cccgaacctg ggccctgccc acctacaaca accacctcta caagcaaatc 780
tcaagccaat caggagcctc aaacgacaac cactactttg gctacagcac cccttggggg 840
tattttgact tcaacagatt ccactgccac ttttcaccac gtgactggca aagactcatc 900
aacaacaact ggggattccg acccaagaga ctcaacttca agctctttaa cattcaagtc 960
aaagaggtca cgcagaatga cggtacgacg acgattgcca ataaccttac cagcacggtt 1020
caggtgttta ctgactcgga gtaccagctc ccgtacgtcc tcgggtcggc gcatcaagga 1080
tgcctcccgc cgtttccagc ggacgtcttc atggtcccac agtatggata cctcaccctg 1140
aacaacggga gtcaggcggt aggacgctct tccttttact gcctggagta ctttccttct 1200
cagatgcttc gtactggaaa caactttcag ttcagctaca cctttgaaga cgttcctttc 1260
cacagcagct acgctcacag ccagagtctg gatcggctga tgaatcctct gatcgaccag 1320
tacctgtatt atctgaacaa aacacaatca aatagtggaa ctcttcagca gtctcggcta 1380
ctgtttagtc aagctggacc caccagcatg tctcttcaag ctaaaaactg gctacctgga 1440
ccttgctaca gacagcagcg tctgtcaaag caggcaaacg acaacaacaa cagcaacttt 1500
ccctggactg cggctacaaa gtaccaccta aatggccggg actcgttggt taatccagga 1560
ccagctatgg ccagtcacaa agacgatgaa gaaaagtttt tccccatgca tggaaccctg 1620
atatttggta aacaaggaac aaatgctaac gacgcggatt tggacaatgt catgattaca 1680
gatgaagaag aaatccgcac caccaatccc gtggctacgg agcagtacgg atatgtgtca 1740
aataatttgc aaaactcaaa tactggtcca actactggaa ctgtcaatca ccaaggagcg 1800
ttacctggca tggtgtggca ggatcgagac gtgtacctgc agggacccat ttgggccaag 1860
attcctcaca ccgatggaca ctttcatcct tctccactga tgggaggttt tgggctcaaa 1920
cacccgcctc ctcagatcat gatcaaaaac actcccgttc cagccaatcc tcccacaaac 1980
ttcagttctg ccaagtttgc ttctttcatc acacagtatt ccacggggca ggtcagcgtg 2040
gagatcgagt gggagctgca gaaagagaac agcaaacgct ggaatcccga aattcagtac 2100
acttccaact acaacaaatc tgttaatgtg gactttactg tggacactaa tggcgtgtat 2160
tcagagcctc gccccattgg caccagatac ctgactcgta atctgtaa 2208
<210>54
<211>2208
<212>DNA
<213>新AAV血清型,克隆rh.56
<400>54
atggctgccg atggttatct tccagattgg ctcgaggaca ctctctctga aggaataaga 60
cagtggtgga agctcaaacc tggcccacca ccaccaaagc ccgcagagcg gcataaggac 120
gacagcaggg gtcttgtgct tcctgggtac aagtacctcg gacccttcaa cggactcgac 180
aagggggagc cggtcaacga ggcagacgcc gcggccctcg agcacgacaa ggcctacgac 240
cggcagctcg acagcggaga caacccgtac ctcaagtaca accacgccga cgcggagttt 300
caggagcgtc ttaaagaaga tacgtctttt gggggcaacc tcggacgagc agtcttccag 360
gcgaaaaaga gggttcttga acctctgggc ctggttgagg aacctgttaa gacggctccg 420
ggaaaaaaga ggccggtaga gcactctcct gcggagccag actcctcctc gggaaccgga 480
aaagcgggcc agcagcctgc aagaaaaaga ttgaattttg gtcagactgg agacgcagac 540
tccgtacctg acccccagcc tctcggacag ccaccagcag ccccctctgg tttgggatct 600
actacaatgg ctacaggcag tggcgcacca atggcagaca ataacgaggg tgccgatgga 660
gtgggtaatt cttcaggaaa ttggcattgc gattcccaat ggctgggcga cagagtcatc 720
accaccagca cccgaacctg ggcccagccc acctacaaca accacctcta caagcaaatc 780
tcaagccaat caggagcctc aaacgacaac cactactttg gctacagcac cccttggggg 840
tattttgact tcaacagatt ccactgccac ttttcaccac gtgactggca aagactcatc 900
aacaacaact ggggattccg acccaagaga ctcaacttca agctctttaa cattcaagtc 960
aaagaggtca cgcagaatga cggtacgacg acgattgcca ataaccttac cagcacggtt 1020
caggtgttta ctgactcgga gtaccagctc ccgtacgtcc tcgggtcggc gcatcaagga 1080
tgcctcccgc cgtttccagc ggacgtcttc atggtcccac agtatggata cctcaccctg 1140
aacaacggga gtcaggcggt aggacgctct tccttttact gcctggagta ctttccttct 1200
cagatgcttc gtactggaaa caactttcag ttcagctaca cctttgaaga cgttcctttc 1260
cacagcagct acgctcacag ccagagtctg gatcggctga tgaatcctct gatcgaccag 1320
tacctgtatt atctgaacaa aacacaatca aatagtggag ctcttcagca gtctcggcta 1380
ctgtttagtc aagctggacc caccagcatg tctcttcaag ctaaaaactg gctacctgga 1440
ccttgctaca gacagcagcg tctgtcaaag caggcaaacg acaacaacaa cagcaacttt 1500
ccctggactg cggctacaaa gtaccaccta aatggccggg actcgttggt taatccagga 1560
ccagctatgg ccagtcacaa agacgatgaa gaaaagtttt tccccatgca tggaaccctg 1620
atatttggta aacaaggaac aaatgctaac gacgcggatt tggacaatgt catgattaca 1680
gatgaagaag aaatccgcac caccaatccc gtggctacgg agcagtacgg atatgtgtca 1740
aataatttgc aaaactcaaa tactggtcca actactggaa ctgtcaatca ccgaggagcg 1800
ttacctggca tggtgtggca ggatcgagac gtgtacctgc agggacccat ttgggccaag 1860
attcctcaca ccgatggaca ctttcatcct tctccactga tgggaggttt tgggctcaaa 1920
cacccgcctc ctcagatcat gatcaaaaac actcccgttc cagccaatcc tcccacaaac 1980
ttcagttctg ccaagtttgc ttctttcatc acacagtatt ccacggggca ggtcagcgtg 2040
gagatcgagt gggagctgca gaaagagaac agcaaacgct ggaatcccga aattcagtac 2100
acttccaact acaacaaatc tgttaatgtg gactttactg tggacactaa tggcgtgtat 2160
tcagagcctc gccccattgg caccagatac ctgactcgta atctgtaa 2208
<210>55
<211>2208
<212>DNA
<213>新AAV血清型,克隆hu.7
<400>55
atggctgccg atggttatct tccagattgg ctcgaggaca ctctctctga aggaataaga 60
cagtggtgga agctcaaacc tggcccacca ccaccaaagc ccgcagagcg gcataaggac 120
gacagcaggg gtcttgtgct tcctgggtac aagtacctcg gacccttcaa cggactcgac 180
aagggggagc cggtcaacga ggcagacgcc gcggccctcg agcacgacaa ggcctacgac 240
cggcagctcg acagcggaga caacccgtac ctcaagtaca accacgccga cgcggagttt 300
caggagcgtc ttaaagaaga tacgtctttt gggggcaacc tcggacgagc agtcttccag 360
gcgaaaaaga gggttcttga acctctgggc ctggttgagg gacctgttaa gacggctccg 420
ggaaaaaaga ggccggtaga gcactctcct gcggagccag actcctcctc gggaaccgga 480
aaagcgggcc agcagcctgc aagaaaaaga ttgaattttg gtcagactgg agacgcagac 540
tccgtacctg acccccagcc tctcggacag ccaccagcag ccccctctgg tttgggatct 600
actacaatgg ctacaggcag tggcgcacca atggcagaca ataacgaggg tgccgatgga 660
gtgggtaatt cctcaggaaa ttggcattgc gattcccaat ggctgggcga cagagtcatc 720
accaccagca cccgaacctg ggccctgccc acctacaaca accacctcta caagcaaatc 780
tcaagccaat caggagcctc aaacgacaac cactactttg gctacagcac cccttggggg 840
tattttgact tcaacagatt ccactgccac ttttcaccac gtgactggca aagactcatc 900
aacaacaact ggggattccg acccaagaga ctcaacttca agctctttaa cattcaagtc 960
aaagaggtca cgcagaatga cggtacgacg acgattgcca ataaccttac cagcacggtt 1020
caggtgttta ctgactcgga gtaccagctc ccgtacgtcc tcgggtcggc gcatcaagga 1080
tgcctcccgc cgtttccagc ggacgtcttc atggtcccac agtatggata cctcaccctg 1140
aacaacggga gtcaggcggt aggacgctct tccttttact gcctggagta ctttccttct 1200
cagatgcttc gtactggaaa caactttcag ttcagctaca cctttgaaga cgttcctttc 1260
cacagcagct acgctcacag ccagagtctg gatcggctga tgaatcctct gatcgaccag 1320
tacctgtatt atctgaacaa aacacaatca aatagtggaa ctcttcagca gtctcggcta 1380
ctgtttagtc aagctggacc caccagcatg tctcttcaag ctaaaaactg gctacctgga 1440
ccttgctaca gacagcagcg tctgtcaaag caggcaaacg acaacaacaa cagcaacttt 1500
ccctggactg cggctacaaa gtatcaccta aatggccggg actcgttggt taatccagga 1560
ccagctatgg ccagtcacaa agacgatgaa gaaaagtttt tccccatgca tggaaccctg 1620
atatttggta aacaaggaac aaatgctaac gacgcggatt tggacaatgt catgattaca 1680
gatgaagaag aaatccgcac caccaatccc gtggctacgg agcagtacgg atatgtgtca 1740
aataatttgc aaaactcaaa tactggtcca actactggaa ctgtcaatca ccaaggagcg 1800
ttacctggca tggtgtggca ggatcgagac gtgtacctgc agggacccat ttgggccaag 1860
attcctcaca ccgatggaca ctttcatcct tctccactga tgggaggttt tgggctcaaa 1920
cacccgcctc ctcagatcat gatcaaaaac actcccgttc cagccaatcc tcccacaaac 1980
ttcagttctg ccaagtttgc ttctttcatc acacagtatt ccacggggca ggtcagcgtg 2040
gagatcgagt gggagctgca gaaagagaac agcaaacgct ggaatcccga aattcagtac 2100
acttccaact acaacaaatc tgttaatgtg gactttactg tggacactaa tggcgtgtat 2160
tcagagcctc gccccattgg caccagatac ctgactcgta atctgtaa 2208
<210>56
<211>2208
<212>DNA
<213>新AAV血清型,克隆hu.10
<400>56
atggctgccg atggttatct tccagattgg ctcgaggaca ctctctctga aggaataaga 60
cagtggtgga agctcaaacc tggcccacca ccaccaaagc tcgcagagcg gcatcaggac 120
gacagcaggg gtcttgtgct tcctgggtac aagtacctcg gacccttcaa cggactcgac 180
aaaggagagc cggtcaacga ggcagacgcc gcggccctcg agcacgacaa ggcctacgac 240
cggcagctcg acagcggaga caacccgtac ctcaagtaca accacgccga cgcggagttt 300
caggagcgtc ttaaagaaga tacgtctttt gggggcaacc tcggacgagc agtcttccag 360
gcgaaaaaga gggttcttga acctctgggc ctggttgagg aacctgttaa gacggctccg 420
ggaaaaaaga ggccggtaga gcactctcct gtggagccag actcctcctc gggaaccgga 480
aaggcgggcc atcagcctgc gagaaagaga ttgaattttg gtcagactgg agacgcagac 540
tccgtacctg acccccagcc tctcggacag ccaccagcag cccccacaag tctgggatct 600
actacaatgg ctacaggcag tggcgcacca atggcagaca ataacgaggg tgccgatgga 660
gtgggtaatt cctcaggaaa ttggcattgc gattcccaat ggctgggcga cagagtcatc 720
accaccagca cccgaacctg ggccctgccc acctacaaca accacctcta caagcaaatc 780
tccagccaat caggagcctc gaacgacaac cactactttg gctacagcac cccctggggg 840
tattttgact tcaacagatt ccactgtcac ttctccccac gtgattggca aagactcatc 900
aacaacaact ggggattccg acccaagaga ctcaacttca agctctttaa cattcaagtc 960
aaagaggtca cgcagaatga cggtacgacg acgattgcca ataaccttac cagcacggtt 1020
caggtgttta ctgactcgga gtaccagctc ccgtacgtcc tcggctcggc gcatcaagga 1080
tgcctcccgc cgtttccagc ggacgtcttc acggtcccac agtatggata cctcaccctg 1140
aacaacggga gtcaggcggt aggacgctct tccttttact gcctggagta ctttccttct 1200
cagatgctgc gtactggaaa caaccttacc ttcagctaca cctttgagga cgttcctttc 1260
cacagcagct acgctcacag ccagagtttg gaccggctga tgaatcctct gatcgaccag 1320
tatctatatt atctgaacag gacacaatca aatagtggaa ctcttcagca gtctaggcta 1380
ctgtttagcc aagctggacc caccagcatg tctcttcaag ctaaaaactg gctgcctgga 1440
ccttgctaca gacagcagcg tctttcaaag caggcaaacg acaacaacaa cagcaacttt 1500
ccctggactg cggctacaaa gtatcatcta aatggccggg actcgttggt taatccagga 1560
ccagctatgg ccagccacaa agacgatgaa gaaaagtttt tccccatgca tggaaccctg 1620
atatttggta aacaaggaac aaatgctaac gacgcggatt tggagcatgt tatgattaca 1680
gatgaagaag aaatcaggac caccaatcct gtggctacag agcagtacgg aaacgtgtca 1740
aataatttgc aaaactcaaa tactggtcca actacagaaa atgtcaatca ccagggagcg 1800
ttacctggta tggtgtggca ggatcgagac gtgtacctgc agggacccat ttgggccaag 1860
attcctcaca ccgacggaca ctttcaccct tctccactga tgggaggttt tggactcaaa 1920
cacccgcctc ctcaaatcat gatcaaaaac actcccgttc cagccaatcc tcccacaaac 1980
tacagttctg ccaagtttgc ttctttcatc acacagtatt ccacgggcca ggtcagcgtg 2040
gagattgagt gggagctgcg gaaggagaac agcaaacgct ggaaccccga gatccagtat 2100
acttccaact acaacaaatc tgttaatgtg gactttactg tggacactaa tggtgtgtat 2160
tcagagcctc gccccattgg caccagatac ctgactcgta atctgtaa 2208
<210>57
<211>2208
<212>DNA
<213>新AAV血清型,克隆hu.11
<400>57
atggctgccg atggttatct tccagattgg ctcgaggaca ctctctctga aggaataaga 60
cagtggtgga agctcaaacc tggcccacca ccaccaaagc ccgcagagcg gcatcaggac 120
gacagcaggg gtcttgtgct tcctgggtac aagtacctcg gacccttcaa cggactcgac 180
aaaggagagc cggtcaacga ggcagacgcc gcggccctcg agcacgacaa ggcctacgac 240
cggcagctcg acagcggaga caacccgtac ctcaagtaca accacgccga cgcggagttt 300
caggagcgtc ttaaagaaga tacgtctttt gggggcaacc tcggacgagc agtcttccag 360
gcgaaaaaga gggttcttga acctctgggc ctggttgagg aacctgttaa gacggctccg 420
ggaaaaaaga ggccggtaga gcactctcct gtggagccag actcctcctc gggaaccgga 480
aaagcgggcc atcagcctgc gagaaagaga ttgaattttg gtcagactgg agacgcagac 540
tccgtacctg acccccagcc tctcggacag ccaccagcag cccccacaag tttgggatct 600
actacaatgg ctacaggcag tggcgcacca atggcagaca ataacgaggg tgccgatgga 660
gtgggtaatt cctcaggaaa ttggcattgc gattcccaat ggctgggcga cagagtcatc 720
accaccagca cccgaacctg ggccctgccc acctacaaca accacctcta caagcaaatc 780
tccagccaat caggagcctc gaacgacaac cactactttg gctacagcac cccctggggg 840
tattttgact tcaacagatt ccactgtcac ttctccccac gtgattggca aagactcatc 900
aacaacaact ggggattccg acccaagaga ctcaacttca agctctttaa cattcaagtc 960
aaggaggtca cgcagaatga cggtacgacg acgattgcca ataaccttac cagcacggtt 1020
caggtgttta ctgactcgga gtaccagctc ccgtacgtcc tcggctcggc gcatcaagga 1080
tgcctcccgc cgtttccagc ggacgtcttc atggtcccac agtatggata cctcaccctg 1140
aacaacggga gtcaggcggt aggacgctct tccttttact gcctggagta ctttccttct 1200
cagatgctgc gtactggaaa caactttacc ttcagctaca cctttgagga cgttcctttc 1260
cacagcagct acgctcacag ccagagtttg gaccggctga tgaatcctct gatcgaccag 1320
tatctatatt atctgaacag gacacaatca aatagtggaa ctcttcagca gtctaggcta 1380
ctgtttagcc aagctggacc caccagcatg tctcttcaag ctaaaaactg gctgcctgga 1440
ccttgctaca gacagcagcg tctttcaaag caggcaaacg acaacaacaa cagcaacttt 1500
ccctggactg cggctacaaa gtatcgtcta aatggccggg actcgttggt taatccagga 1560
ccagctatgg ccagccacaa agacgatgaa gaaaagtttt tccccatgca tggaaccctg 1620
atatttggta aacaaggaac aaatgctaac gacgcggatt tggagcatgt tatgattaca 1680
gatgaagaag aaatcaggac caccaatcct gtggctacag agcagtacgg aaacgtgtca 1740
aataatttgc aaaactcaaa tactggtcca actacagaaa atgtcaatca ccagggagcg 1800
ttacctggta tggtgtggca ggatcgagac gtgtacctgc agggacccat ttgggccaag 1860
attcctcaca ccgacggaca ctttcaccct tctccactga tgggaggttt tggactcaaa 1920
cacccgcctc ctcaaatcat gatcaaaaac actcccgttc cagccaatcc tcccacaaac 1980
ttcagttctg ccaagtttgc ttctttcatc acacagtatt ccacgggcca ggtcagcgtg 2040
gagattgagt gggagctgca gaaggagaac agcaaacgct ggaaccccga gatccagtat 2100
acttccaact acaacaaatc tgttaatgtg gactttactg tggacactaa tggtgtgtat 2160
tcagagcctc gccccattgg caccagatac ctgactcgta atctgtaa 2208
<210>58
<211>2208
<212>DNA
<213>新AAV血清型,克隆hu.9
<400>58
atggctgccg atggttatct tccagattgg ctcgaggaca ctctctctga aggaataaga 60
cagtggtgga agctcaaacc tggcccacca ccaccaaagc ccgcagagcg gcatcaggac 120
aacagcaggg gtcttgtgct tcctgggtac aagtacctcg gaccctccaa cggactcgac 180
aaaggagagc cggtcaacga ggcagacgcc gcggccctcg agcacgacaa ggcctacgac 240
cggcagctcg acagcggaga caacccgtac ctcaagtaca accacgccga cgcggagttt 300
caggagcgtc ttaaagaaga tacgtctttt gggggcaacc tcggacgagc agtcttccag 360
gcgaaaaaga gggttcttga acctctgggc ctggttgagg aacctgttaa gacggctccg 420
ggaaaaaaga ggccggtaga gcactctcct gtggagccag actcctcctc gggaaccgga 480
aaagcgggcc atcagcctgc gagaaagaga ttgaattttg gtcagactgg agacgcagac 540
tccgtacctg acccccagcc tctcggacag ccaccagcag cccccacaag tttgggatct 600
actacaatgg ctacaggcag tggcgcacca atggcagaca ataacgaggg tgccgatgga 660
gtgggtaatt cctcaggaaa ttggcattgc gattcccaat ggctgggcga cagagtcatc 720
accaccagca cccgaacctg ggccctgccc acctacaaca accacctcta caagcaaatc 780
tccagccaat caggagcctc gaacgacaac cactactttg gctgcagcac cccctggggg 840
tattttgact tcaacagatt ccactgtcac ttctccccac gtgattggca aagactcatc 900
aacaacaact ggggattccg acccaagaga ctcaacttca agctctttaa cattcaagtc 960
aaagaggtca cgcagaatga cggtacgacg acgattgcca ataaccttac cagcacggtt 1020
caggtgttta ctgactcgga gtacccgctc ccgtacgtcc tcggctcggc gcatcaagga 1080
tgcctcccgc cgtttccagc ggacgtcttc atggtcccac agtatggata cctcaccctg 1140
aacaacggga gtcaggcggt aggacgctct tccttttact gcctggagta ctttccttct 1200
cagatgctgc gtaccggaaa caactttacc ttcagctaca cctttgagga cgttcctttc 1260
cacagcagct acgctcacag ccagagtttg gaccggctga tgaatcctct gatcgaccag 1320
tatctatatt atctgaacag gacacaatca aatagtggaa ctcttcagca gtctaggcta 1380
ctgtttagcc aagctggacc caccagcatg tctcttcaag ctaaaaactg gctgcctgga 1440
ccttgctaca gacagcagcg tctttcaaag caggcaaacg acaacaacaa cagcaacttt 1500
ccctggactg cggctacaaa gtatcatcta aatggccggg actcgttggt taatccagga 1560
ccagctatgg ccagccacaa agacgatgaa gaaaagtttt tccccatgca tggaaccctg 1620
atatttggta aacaaggaac aaatgctaac gacgcggatt tggagcatgt tatgattaca 1680
gatgaagaag aaatcaggac caccaatcct gtggctacag agcagtacgg aaacgtgtca 1740
aataatttgc aaaactcaaa tactggtcca actacagaaa atgtcaatca ccagggagcg 1800
ttacctggta tggtgtggca ggatcgagac gtgtacctgc agggacccat ttgggccaag 1860
attcctcaca ccgacggaca ctttcaccct tctccactga tgggaggttt tggactcaaa 1920
cacccgcctc ctcaaatcat gatcaaaaac actcccgttc cagccaatcc tcccacaaac 1980
ttcagttctg ccaagtttgc ttctttcatc acacagtatt ccacgggcca ggtcagcgtg 2040
gagattgagt gggagctgca gaaggagaac agcaaacgct ggaaccccga gatccagtat 2100
acttccaact acaacaaatc tgttaatgtg gactttactg tggacactaa tggtgtgtat 2160
tcagagcctt gccccattgg caccagatac ctgactcgta atctgtaa 2208
<210>59
<211>2208
<212>DNA
<213>新AAV血清型,克隆hu.12
<400>59
atggctgccg atggttatct tccagattgg ctcgaggaca ctctctctga aggaataaga 60
cagtggtgga agctcaaacc tggcccacca ccaccaaagc ccgcagagcg gcatcaggac 120
gacagcaggg gtcttgtgct tcctgggtac aagtacctcg gacccttcaa cggactcgac 180
aaaggagagc cggtcaacga ggcagacgcc gcggccctcg agcacgacaa ggcctacgac 240
cggcagctcg acagcggaga caacccgtac ctcaagtaca accacgccga cgcggagttt 300
caggagcgtc ttaaagaaga tacgtctttt gggggcaacc tcggacgagc agtcttccag 360
gcgaaaaaga gggttcttga acctctgggc ctggttgagg aacctgttaa gacggctccg 420
ggaaaaaaga ggccggtaga gcactctcct gtggagccag actcctcctc gggaaccgga 480
aaagcgggcc atcagcctgc gagaaagaga ttgaattttg gtcagactgg agacgcagac 540
tccgtacctg acccccagcc tctcggacag ccaccagcag cccccacaag tttgggatct 600
actacaatgg ctacaggcag tggcgcacca atggcagaca ataacgaggg tgccgatgga 660
gtgggtaatt cctcaggaaa ttggcattgc gattcccaat ggctgggcga cagagtcatc 720
accaccagca cccgaacctg ggccctgccc acctacaaca accacctcta caagcaaatc 780
tccagccaat caggagcctc gaacgacaac cactactttg gctacagcac cccctggggg 840
tattttgact tcaacagatt ccactgtcac ttctccccac gtgattggca aagactcatc 900
aacaacaact ggggattccg acccaagaga ctcaacttca agctctttaa cattcaagtc 960
aaagaggtca cgcagaatga cggtacgacg acgattgcca ataaccttac cagcacggtt 1020
caggtgttta ctgactcgga gtaccagctc ccgtacgtcc tcggctcggc gcatcaagga 1080
tgcctcccgc cgtttccagc ggacgtcttc atggtcccac agtatggata cctcaccctg 1140
aacaacggga gtcaggcggt aggacgccct tccttttact gcctggagta ctttccttct 1200
cagatgctgc gtactggaaa caactttacc ttcagctaca cctttgagga cgttcctttc 1260
cacagcagct acgctcacag ccagagtttg gaccggctga tgaatcctct gatcgaccag 1320
tatctatatt atctgaacag gacacaatca aatagtggaa ctcttcagca gtctaggcta 1380
ctgtttagcc aagctggacc caccagcatg tctcttcaag ctaaaaactg gctgcctgga 1440
ccttgctaca gacagcagcg tctttcaaag caggcaaacg acaacaacaa cagcaacttt 1500
ccctggactg cggctacaaa gtatcatcta aatggccggg actcgttggt taatccagga 1560
ccagctatgg ccagccacaa agacgatgaa gaaaagtttt tccccatgca tgggaccctg 1620
atatttggta aacaaggaac aaatgctaac gacgcggatt tggagcatgt tatgattaca 1680
gatgaagaag aaatcaggac caccaatcct gtggctacag agcagtacgg aaacgtgtca 1740
aataatttgc aaaactcaaa tactggtcca actacagaaa atgtcaatca ccagggagcg 1800
ttacctggta tggtgtggca ggatcgagac gtgtacctgc agggacccat ttgggccaag 1860
attcctcaca ccgacggaca ctttcaccct tctccactga tgggaggttt tggactcaaa 1920
cacccgcctc ctcaaatcat gatcaaaaac actcccgttc cagccaatcc tcccacaaac 1980
ttcagttctg ccaagtttgc ttctttcatc acacagtatt ccacgggcca ggtcagcgtg 2040
gagattgagt gggagctgca gaaggagaac agcaaacgct ggaaccccga gatccagtat 2100
acttccaact acaacaaatc tgttaatgtg gactttactg tggacactaa tggtgtgtat 2160
tcagagcctc gccccattgg caccagatac ctgactcgta atctgtaa 2208
<210>60
<211>2208
<212>DNA
<213>新AAV血清型,克隆hu.23
<400>60
atggctgccg atggttatct tccagattgg ctcgaggaca ctctctctga aggaataaga 60
cagtggtgga agctcaaacc tggcccacca ccaccaaagc ccgcagagcg gcataaggac 120
gacagcaggg gtcttgtgct tcctgggtac aagtacctcg gacccttcaa cggactcgac 180
aagggagagc cggtcaacga ggcagacgcc gcggccctcg agcacgacaa ggcctacgac 240
cggcagctcg acagcggaga caacccgtac ctcaagtaca accacgccga cgcggagttt 300
caggagcgtc ttaaagaaga tacgtctttt gggggcaacc tcggacgagc agtcttccag 360
gccaaaaaga ggattcttga acctctgggc ctggttgagg aacctgttaa gacggctccg 420
ggaaaaaaga ggccggtaga gcactctcct gcggagccag actcctcctc gggaaccgga 480
aaagcgggcc agcagcctgc aagaaagaga ttgaattttg gtcagactgg agacgcagac 540
tcagtacctg acccccagcc tctcggacag ccaccagcag ccccctctgg tctgggaact 600
aatacgatgg cttcaggcag tggcgcacca atggcagaca ataacgaggg cgccgacgga 660
gtgggtaatt cctcgggaaa ttggcattgc gattccacat ggatgggcga cagagtcatc 720
accaccagca cccgcacctg ggccctgccc acctgcaaca accatctgta caagcaaatc 780
tccagccagt ctggagccag caacgacaac cactactttg gctacagcac cccctggggg 840
tattttgact tcaacagatt ccactgccac ttctccccac gtgactggca aagactcatc 900
aacaacaact ggggattccg gcccaagaga ctcagcttca agctctttaa cattcaagtc 960
aaagaggtca cgcagaatga cggtacgacg acgattgcca ataaccttac cagcacggtt 1020
caggtgttta ctgactcgga gtaccagctc ccgtacgtcc tcggctcggc gcatcaagga 1080
tgcctcccgc cgttcccagc agacgtcttc atggtgccac agtatggata cctcaccctg 1140
aacaacggca gtcaggcggt aggacgctct tccttttact gcctggagta ttttccttct 1200
cagatgcttc gtaccggaaa caactttacc ttcagctaca cctttgaaga cgttcctttc 1260
catagcagct acgctcacag ccaaagtctg gaccgtctca tgaatcctct catcgaccag 1320
tacctgtatt acttgagcag aacaaacact ccaagcggaa ccaccacgat gtccaggctt 1380
cagttttctc aggccggagc aagtgacatt cgggaccagt ctagaaactg gcttcctgga 1440
ccctgttacc gccagcagcg agtatcaaag acagctgcgg acaacaacaa cagtgattac 1500
tcgtggactg gagctaccaa gtaccacctc aatggaagag actctctggt gaatccgggc 1560
ccagctatgg ccagccacaa ggacgatgaa gaaaaatatt ttcctcagag cggggttctc 1620
atctttggaa aacaagactc gggaaaaact aatgtggaca ttgaaaaggt tatgattaca 1680
gacgaagagg aaatcaggac caccaatccc gtggctacgg agcagtatgg ttctgtatct 1740
acctacctcc agagcggcaa cacacaagca gctacctcag atgtcaacac acaaggcgtt 1800
cttccaggca tggtctggca ggacagagac gtgtacctgc gggggcccat ctgggcaaag 1860
attccacaca cggacggaca ttttcacccc tctcccctca tgggcggatt tggacttaaa 1920
caccctcctc cacaaattct catcaagaac accccggtac ctgcgaatcc ttcgaccact 1980
ttcagtgcgg caaagtttgc ttccttcatc acacagtact ccacggggca ggtcagcgtg 2040
gagatcgagt gggagctgca gaaggagaac agcaaacgct ggaatcccga aattcagtac 2100
acttccaact acaacaaatc tgttaatgtg gactttactg tggacactaa tggtgtgtat 2160
tcagagcctc gccccattgg caccagatac ctgactcgta atctgtaa 2208
<210>61
<211>2208
<212>DNA
<213>新AAV血清型,克隆hu.26
<400>61
atggctgccg atggttatct tccagattgg ctcgaggaca ctctctctga aggaataaga 60
cagtggtgga agctcaaacc tggcccacca ccaccaaagc ccgcagagcg gcataaggac 120
gacagcaggg gtcttgtgct tcctgggtac aagtacctcg gacccttcaa cggactcgac 180
aagggagagc cggtcaacga ggcagacgcc gcggccctcg agcacgacaa ggcctacgac 240
cggcagctcg acagcggaga caacccgtac ctcaagtaca accacgccga cgcggagttt 300
caggagcgtc ttaaagaaga tacgtctttt gggggcaacc tcggacgagc agtcttccag 360
gccaaaaaga ggattcttga acctctgggc ctggttgagg aacctgttaa gacggctccg 420
ggaaaaaaga ggccggtaga gcactctcct gcggagccag actcctcctc gggaaccgga 480
aaagcgggcc agcagcctgc aagaaagaga ttgaattttg gtcagactgg agacgcagac 540
tcagtacctg acccccagcc tctcggacag ccaccagcag ccccctctgg tctgggaact 600
aatacgatgg cttcaggcag tggcgcacca atggcagaca ataacgaggg cgccgacgga 660
gtgggtaatt cctcgggaaa ttggcattgc gattccacat ggatgggcga cagagtcatc 720
accaccagca cccgcacctg ggccctgccc acctacaaca accatctgta caagcaaatc 780
tccagccagt ctggagccag caacgacaac cactactttg gctacagcac cccctggggg 840
tattttgact tcaacagatt ccactgccac ttctccccac gtgactggca aagactcatc 900
aacaacaact ggggattccg gcccaagaga ctcagcttca agctctttaa cattcaagtc 960
aaagaggtca cgcagaatga cggtacgacg acgattgcca ataaccttac cagcacggtt 1020
caggtgttta ctgactcgga gtaccagctc ccgtacgtcc tcggctcggc gcatcaagga 1080
tgcctcccgc cgttcccagc agacgtcttc atggtgccac agtatggata cctcaccctg 1140
aacaacggca gtcaggcggt aggacgctct tccttttact gcctggagta ctttccttct 1200
cagatgcttc gtaccggaaa caactttacc ttcagctaca cctttgaaga cgttcctttc 1260
catagcagct acgctcacag ccaaagtctg gaccgtctca tgaatcctct catcgaccag 1320
tacctgtatt acttgagcag aacaaacact ccaagcggaa ccaccacgat gtccaggctt 1380
cagttttctc aggccggagc aagtgacatt cgggaccagt ctagaaactg gcttcctgga 1440
ccctgttacc gccagcagcg agtatcaaag acagctgcgg acaacaacaa cagtgattac 1500
tcgtggactg gagctaccaa gtaccacctc aatggaagag actctctggt gaatccgggc 1560
ccagctatgg ccagccacaa ggacgatgaa gaaaaatatt ttcctcagag cggggttctc 1620
atctttggaa aacaagactc gggaaaaact aatgtggaca ttgaaaaggt tatgattaca 1680
gacgaagagg aaatcaggac caccaatccc gtggctacgg agcagtatgg ttctgtatct 1740
accaacctcc agagcggcaa cacacaagca gctacctcag atgtcaacac acaaggcgtt 1800
cttccaggca tggtctggca ggacagagac gtgtacctgc aggggcccat ctgggcaaag 1860
attccacaca cggacggaca ttttcacccc tctcccctca tgggcggatt cggacttaaa 1920
caccctcctc cacaaattct catcaagaac accccggtac ctgcgaatcc ttcgaccact 1980
ttcagtgcgg caaagtttgc ttccttcatc acacagtact ccacggggca ggtcagcgtg 2040
gagatcgagt gggagctgca gaaggagaac agcaaacgct ggaatcccga aattcagtac 2100
acttccaact acaacaaatc tgttaatgtg gactttactg tggacactaa tggtgtgtat 2160
tcagagcctc gccccattgg caccagatac ctgactcgta atctgtaa 2208
<210>62
<211>2208
<212>DNA
<213>新AAV血清型,克隆hu.19
<400>62
atggctgccg atggttatct tccagattgg ctcgaggaca ctctctctga aggaataaga 60
cagtggtgga agctcaaacc tggcccacca ccaccaaagc ccgcagagcg gcataaggac 120
gacagcaggg gtcttgtgct tcctgggtac aagtacctcg gacccttcaa cggactcgac 180
aagggagagc cggtcaacga ggcagacgcc gcggccctcg agcacgacaa ggcctacgac 240
cggcagctcg acagcggaga caacccgtac ctcaagtaca accacgccga cgcggagttt 300
caggagcgtc ttaaagaaga tacgtctttt gggggcaacc tcggacgagc agtcttccag 360
gccaaaaaga ggattcttga acctctgggc ctggttgagg aacctgttaa gacggctccg 420
ggagaaaaga ggccggtaga gcactctcct gcggagccag actcctcctc gggaaccgga 480
aaagcgggcc agcagcctgc aagaaagaga ttgaattttg gccagactgg agacgcagac 540
tcagtacctg acccccagcc tctcggacag ccaccagcag ccccctctgg tctgggaact 600
aatacgatgg cttcaggcag tggcgcacca atggcagaca ataacgaggg cgccgacgga 660
gtgggtaatt cctcgggaaa ttggtattgc gattccacat ggatgggcga cagagtcatc 720
accaccagca cccgcacctg ggccctgccc acctacaaca accatctgta caagcaaatc 780
tccagccagt ctggagccag caacgacaac cactactttg gctacagcac cccctggggg 840
tattttgact tcaacagatt ccactgccac ttctccccac gtgactggca aagactcatc 900
aacaacaact ggggattccg gcccaagaga ctcagcttca agctctttaa cattcaagtc 960
aaagaggtca cgcagaatga cggtacgacg acgattgcca ataaccttac cagcacggtt 1020
caggtgttta ctgactcgga gtaccagctc ccgtacgtcc tcggctcggc gcatcaagga 1080
tgcctcccgc cgttcccagc agacgtcttc atggtgccac agtatggata cctcaccctg 1140
aacaacggca gtcaggcggt aggacgctct tccttttact gcctggagta ctttccttct 1200
cagatgcttc gtaccggaaa caactttacc ttcagctaca cctttgaaga cgttcctttc 1260
catagcagct acgctcacag ccaaagtctg gaccgtctca tgaatcctct catcgaccag 1320
tacctgtatt acttgagcag aacaaacact ccaagcggaa ccaccacgat gtccaggctt 1380
cagttttctc aggccggagc aagtgacatt cgggaccagt ctagaaactg gcttcctgga 1440
ccctgttacc gccagcagcg agtatcaaag acagctgcgg acaacaacaa cagtgattac 1500
tcgtggactg gagctaccaa gtaccacctc aatggaagag actctctggt gaatccgggc 1560
ccagctatgg ccagccacaa ggacgatgaa gaaaaatatt ttcctcagag cggggttctc 1620
atctttggaa aacaagactc gggaaaaact aatgtggaca ttgaaaaggt tatgattaca 1680
gacgaagagg aaatcaggac caccaatccc gtggctacgg agcagtatgg ttctgtatct 1740
accaacctcc agagcggcaa cacacaagca gctacctcag atgtcaacac acaaggcgtt 1800
cttccaggca tggtctggca ggacagagac gtgtacctgc aggggcccat ctgggcaaag 1860
attccacaca cggacggaca ttttcacccc tctcccctcg tgggcggatt cggacttaaa 1920
caccctcctc cacaaattct catcaagaac accccggtac ctgcgaatcc ttcgaccact 1980
ttcagtgcgg caaagtttgc ttccttcatc acacagtact ccacggggca ggtcagcgtg 2040
gagatcgagt gggagctgca gaaggagaac agcaaacgct ggaatcccga aattcagtac 2100
acttccaact acaacaaatc tgttaatgtg gactttactg tggacactaa tggtgtgtat 2160
tcagagcctc gccccattgg caccagatac ctgactcgta atctgtaa 2208
<210>63
<211>2208
<212>DNA
<213>新AAV血清型,克隆hu.20
<400>63
atggctgccg atggttatct tccagattgg ctcgaggaca ctctctctga aggaataaga 60
cagtggtgga agctcaaacc tggcccacca ccaccaaagc ccgcagagcg gcataaggac 120
gacagcaggg gtcttgtgct tcctgggtac aggtacctcg gacccttcaa cggactcgac 180
aagggagagc cggtcaacga ggcagacgcc gcggccctcg agcacgacaa ggcctacgac 240
cggcagctcg acagcggaga caacccgtac ctcaagtaca accacgtcga cgcggagttt 300
caggagcgtc ttaaagaaga tacgtctttt gggggcaacc tcggacgagc agtcttccag 360
gccaaaaaga ggattcttga acctctgggc ctggttgagg aacctgttaa ggcggctccg 420
ggagaaaaga ggccggtaga gcactctcct gcggagccag actcctcctc gggaaccgga 480
aaagcgggcc agcagcctgc aagaaagaga ttgaattttg gtcagactgg agacgcagac 540
tcagtacctg acccccagcc tctcggacag ccaccagcag ccccctctgg tctgggaact 600
aatacgatgg cttcaggcag tggcgcacca atggcagaca ataacgaggg cgccgacgga 660
gtgggtaatt cctcgggaaa ttggcattgc gattccacat ggatgggcga cagagtcatc 720
accaccagca cccgcacctg ggccctgccc acctacaaca accatctgta caagcaaatc 780
tccagccagt ctggagccag caacgacaac cactactttg gctacagcac cccctggggg 840
cattttgact tcaacagatt ccactgccac ttctccccac gtgactggca aagactcatc 900
aacaacaact ggggattccg gcccaagaga ctcagcttca agctctttaa cattcaagtc 960
aaagaggtca cgcagaatga cggtacgacg acgattgcca ataaccttac cagcacggtt 1020
caggtgttta ctgactcgga gtaccagctc ccgtacgtcc tcggctcggc gcatcaagga 1080
tgcctcccgc cgttcccagc agacgtcttc atggtgccac agtatggata cctcaccctg 1140
aacaacggca gtcaggcggt aggacgctct tccttttact gcctggagta ctttccttct 1200
cagatgcttc gtaccggaaa caactttacc ttcagctaca cctttgaaga cgttcctttc 1260
catagcagct acgctcacag ccaaagtctg gaccgtctca tgaatcctct catcgaccag 1320
tacctgtatt acttgagcag aacaaacact ccaagcggaa ccaccacgat gtccaggctt 1380
cagttttctc aggccggagc aagtgacatt cgggaccagt ctagaaactg gcttcctgga 1440
ccctgttacc gccagcagcg agtatcaaag acagctgcgg acaacaacaa cagtgattac 1500
tcgtggactg gagctaccaa gtaccacctc aatggaagag actctctggt gaatccgggc 1560
ccagctatgg ccagccacaa ggacgatgaa gaaaaatatt ttcctcagag cggggttctc 1620
atctttggaa aacaagactc gggaaaaact aatgtggaca ttgaaaaggt tatgattaca 1680
gacgaagagg aaatcaggac caccaatccc gtggctacgg agcagtatgg ttctgtatct 1740
accaacctcc agagcggcaa cacacaagca gctacctcag atgtcaacac acaaggcgtt 1800
cttccaggca tggtctggca ggacagagac gtgtacctgc aggggcccat ctgggcaaag 1860
attccacaca cggacggaca ttttcacccc tctcccccca tgggcggatt cggacttaaa 1920
caccctcctc cacaaattct catcaagaac accccggtac ctgcgaatcc ttcgaccact 1980
ttcagtgcgg caaagtttgc ttccttcatc acacagtact ccacggggca ggtcagcgtg 2040
gagatcgagt gggagctgca gaaggagaac agcaaacgct ggaatcccga aattcagtac 2100
acttccaact acaacaaatc tgttaatgtg gactttactg tggacactaa tggtgtgtat 2160
tcagagcctc gccccattgg cgccagatac ctgactcgta atctgtaa 2208
<210>64
<211>2208
<212>DNA
<213>新AAV血清型,克隆hu.27
<400>64
atggctgccg atggttatct tccagattgg ctcgaggaca ctctctctga aggaataaga 60
cagtggtgga agctcaaacc tggcccacca ccaccaaagc ccgcagagcg gcataaggac 120
gacagcaggg gtcttgtgct tcctgggtac aagtacctcg gacccttcaa cggactcgac 180
aagggagagc cggtcaacga ggcagacgcc gcggccctcg agcacgacaa ggcctacgac 240
cggcagctcg acagcggaga caacccgtac ctcaagtaca accacgccga cgcggagttt 300
caggagcgtc ttaaagaaga tacgtctttt gggggcaacc tcggacgagc agtcttccag 360
gccaaaaaga ggattcttga acctctgggc ctggttgagg aacctgttaa gacggctccg 420
ggaaaaaaga ggccggtaga gcactctcct gcggagccag actcctcctc gggaaccgga 480
aaagcgggcc agcagcctgc aagaaagaga ttgaattttg gtcagactgg agacgcagac 540
tcagtacctg acccccagcc tctcggacag ccaccagcag ccccctctgg tctgggaact 600
aatacgatgg cttcaggcag tggcgcacca atggcagaca ataacgaggg cgccgacgga 660
gtgggtaatt cctcgggaaa ttggcattgc gattccacat ggatgggcga cagagtcatc 720
accaccagca cccgcacctg ggccctgccc acctacaaca accatctgta caagcaaatc 780
tccagccagt ctggagccag caacgacaac cactactttg gctacagcac cccctggggg 840
tatttcgact tcaacagatt ccactgccac ttctccccac gtgactggca aagactcatc 900
aacaacaact ggggattccg gcccaagaga ctcagcttca agctctttaa cattcaagtc 960
aaagaggtca cgcagaatga cggtacgacg acgattgcca ataaccttac cagcacggtt 1020
caggtgttta ctgactcggg gtaccagctc ccgtacgtcc tcggctcggc gcatcaagga 1080
tgccttccgc cgttcccagc agacgtcttc atggtgccac agtatggata cctcaccctg 1140
aacaacggca gtcaggcggt aggacgctct tccttttact gcctggagta ctttccttct 1200
cagatgcttc gtaccggaaa caactttacc ttcagctaca cctttgaaga cgttcctttc 1260
catagcagct acgctcacgg ccaaagtctg gaccgtctca tgaatcctct catcgaccag 1320
tacctgtatt acttgagcag aacaaacact ccaagcggaa ccaccacgat gtccaggctt 1380
cagttttctc aggccggagc aagtgacgtt cgggaccagt ctagaaactg gcttcctgga 1440
ccctgttacc gccagcagcg agtatcaaag acagctgcgg acaacaacaa cagtgattac 1500
tcgtggactg gagctaccaa gtaccacctc aatggaagag actctctggt gaatccgggc 1560
ccagctatgg ccagccacaa ggacgatgaa gaaaaatatt ttcctcagag cggggttctc 1620
gtctttggaa aacaagactc gggaaaaact aatgtggaca ttgaaaaggt tatgattaca 1680
gacgaagagg aaatcaggac caccaatccc gcggctacgg agcagtatgg ttctgtatct 1740
accaacctcc agagcggcaa cacacaagca gctacctcag atgtcaacac acaaggcgtt 1800
cttccaggca tggtctggca ggacagagac gtgtacctgc aggggcccat ctgggcaaag 1860
attccacaca cggacggaca ttttcacccc tctcccctca tgggcggatt cggacttaaa 1920
caccctcctc cacaaattct catcaagaac accccggtac ctgcgaatcc ttcgaccact 1980
ttcagtgcgg caaagtttgt ttccttcatc acacagtact ccacggggca ggtcagcgtg 2040
gagatcgagt gggagctgca gaaggagaac agcaaacgct ggaatcccga aattcagtac 2100
acttccaact acaacaaatc tgttaatgtg gactttactg tggacactaa tggtgtgtat 2160
tcagagcctc gccccattgg caccagatac ctgactcgta atctgtaa 2208
<210>65
<211>2208
<212>DNA
<213>新AAV血清型,克隆hu.21
<400>65
atggctgccg atggttatct tccagattgg ctcgaggaca ccctctctga aggaataaga 60
cagtggtgga agctcaaacc tggcccacca ccaccaaagc ccgcagagcg gcataaggac 120
gacagcaggg gtcttgtgct tcctgggtac aagtacctcg gacccttcaa cggactcgac 180
aagggagagc cggtcaacga ggcagacgcc gcggccctcg agcacgacaa ggcctacgac 240
cggcagctcg acagcggaga taacccgtac ctcaagtaca accacgccga cgcggagttt 300
caggagcgcc ttaaagagga tacgtctttt gggggcaacc tcggacgagc agtcttccag 360
gccaaaaaga ggattcttga acctctgggc ctggttgagg aacctgttaa gacggctccg 420
ggaaaaaaga ggccggtaga gcactctcct gcggagccag actcctcctc gggaaccgga 480
aaagcgggcc agcagcctgc aagaaagaga ttgaattttg gtcagactgg agacgcagac 540
tcagtacctg acccccggcc tctcggacag ccaccagcag ccccctctgg tctgggaact 600
aatacgatgg cttcaggcag tggcgcacca atggcagaca ataacgaggg cgccgacgga 660
gtgggtaatt cctcgggaaa ttggcattgc gattccacat ggatgggcga cagagtcatc 720
accaccagca cccgcacctg ggccctgccc acctacaaca accatctgta caagcaaatc 780
tccagccagt ctggagccag caacgacaac cactactttg gctacagcac cccctggggg 840
tattttgact tcaacagatt ccactgccac ttctccccac gtgactggca aagactcatc 900
aacaacaact ggggattccg gcccaagaga ctcagcttca agctctttaa cattcaagtc 960
aaagaggtca cgcagaatga cggtacgacg acgattgcca ataaccttac cagcacggtt 1020
caggtgttta ctgactcgga gtaccagctc ccgtacgtcc tcggctcggc gcatcaagga 1080
tgcctcccgc cgttcccagc agacgtcttc atggtgccac agtatggata cctcaccctg 1140
aacaacggca gtcaggcggt aggacgctct tccttttact gcctggagta ctttccttct 1200
cagatgcttc gtaccggaaa caactttacc ttcagctaca cctttgaaga cgttcctttc 1260
catagcagct acgctcacag ccaaagtctg gaccgtctca tgaatcctct catcgaccag 1320
tacctgtatt acttgagcag aacaaacact ccaagcggaa ccaccacgat gtccaggctt 1380
cagttttctc aggccggagc aagtgacatt cgggaccagt ctagaaactg gcttcctgga 1440
ccctgttacc gccagcagcg agtatcaaag acagctgcgg acaacaacaa cagtgattac 1500
tcgtggactg gagctaccaa gtaccacctc aatggaagag actctctggt gaatccgggc 1560
ccagctatgg ccagccacaa ggacgatgaa gaaaaatatt ttcctcagag cggggttctc 1620
atctttggaa aacaagactc gggaaaaact aatgtggaca ttgaaaaggt tatgattaca 1680
gacgaagagg aaatcaggac caccaatccc gtggctacgg agcagtatgg ttctgtatct 1740
accaacctcc agagcggcaa cacacaagca gctacctcag atgtcaacac acaaggcgtt 1800
cttccaggca tggtctggca ggacagagac gtgtacctgc aggggcccat ctgggcaaag 1860
attccacaca cggacggaca ttttcacccc tctcccctca tgggcggatt cggacttaaa 1920
caccctcctc cacaaattct catcaagaac accccggtac ctgcgaatcc ttcgaccact 1980
ttcagtgcgg caaagtttgc ttccttcatc acacagtact ccacggggca ggtcagcgtg 2040
gagatcgagt gggagctgca gaaggagaac agcaaacgct ggaatcccga aattcagtac 2100
acttccaact acaacaaatc tgttaatgtg gactttactg tggacactaa tggtgtgtat 2160
tcagagcctc gccccattgg caccagatac ctgactcgta atctgtaa 2208
<210>66
<211>2208
<212>DNA
<213>新AAV血清型,克隆hu.24
<400>66
atggctgccg atggttatct tccagattgg ctcgaggaca ccctctctga aggaataaga 60
cagtggtgga agctcaaacc tggcccacca ccaccaaagc ccgcagagcg gcataaggac 120
gacagcaggg gtcttgtgct tcctgggtac aagtacctcg gacccttcaa cggactcgac 180
aagggagagc cggtcaacga ggcagacgcc gcggccctcg agcacgacaa ggcctacgac 240
cggcagctcg acagcggaga taacccgtac ctcaagtaca accacgccga cgcggagttt 300
caggagcgcc ttaaagagga tacgtctttt gggggcaacc tcggacgagc agtcttccag 360
gccaaaaaga ggattcttga acctctgggc ctggttgagg aacctgttaa gacggctccg 420
ggaaaaaaga ggccggtaga gcactctcct gcggagccag actcctcctc gggaaccgga 480
aaagcgggcc agcagcctgc aagaaagaga ttgaattttg gtcagactgg agacgcagac 540
tcagtacctg acccccggcc tctcggacag ccaccagcag ccccctctgg tctgggaact 600
aatacgatgg cttcaggcag tggcgcacca atggcagaca ataacgaggg cgccgacgga 660
gtgggtaatt cctcgggaaa ttggcattgc gattccacat ggatgggcga cagagtcatc 720
accaccagca cccgcacctg ggccctgccc acctacaaca accatctgta caagcaaatc 780
tccagccagt ctggagccag caacgacaac cactactttg gctacagcac cccctggggg 840
tattttgact tcaacagatt ccactgccac ttctccccac gtgactggca aagactcatc 900
aacaacaact ggggattccg gcccaagaga ctcagcttca agctctttaa cattcaagtc 960
aaagaggtca cgcagaatga cggtacgacg acgattgcca ataaccttac cagcacggtt 1020
caggtgttta ctgactcgga gtaccagctc ccgtacgtcc tcggctcggc gcatcaagga 1080
tgcctcccgc cgttcccagc agacgtcttc atggtgccac agtatggata cctcaccctg 1140
aacaacggca gtcaggcggt aggacgctct tccttttact gcctggagta ctttccttct 1200
cagatgcttc gtaccggaaa caactttacc ttcagctaca cctttgaaga cgttcctttc 1260
catagcagct acgctcacag ccaaagtctg gaccgtctca tgaatcctct catcgaccag 1320
tacctgtatt acttgagcag aacaaacact ccaagcggaa ccaccacgat gtccaggctt 1380
cagttttctc aggccggagc aagtgacatt cgggaccagt ctagaaactg gcttcctgga 1440
ccctgttacc gccagcagcg agtatcaaag acagctgcgg acaacaacaa cagtgattac 1500
tcgtggactg gagctaccaa gtaccacctc aatggaagag actctctggt gaatccgggc 1560
ccagctatgg ccagccacaa ggacgatgaa gaaaaatatt ttcctcagag cggggttctc 1620
atctttggaa aacaagactc gggaaaaact aatgtggaca ttgaaaaggt tatgattaca 1680
gacgaagagg aaatcaggac caccaatccc gtggctacgg agcagtatgg ttctgtatct 1740
accaacctcc agagcggcaa cacacaagca gctacctcag atgtcaacac acaaggcgtt 1800
cttccaggca tggtctggca ggacagagac gtgtacctgc aggggcccat ctgggcaaag 1860
attccacaca cggacggaca ttttcacccc tctcccctca tgggcggatt cggacttaaa 1920
caccctcctc cacaaattct catcaagaac accccggtac ctgcgaatcc ttcgaccact 1980
ttcagtgcgg caaagtttgc ttccttcatc acacagtact ccacggggca ggtcagcgtg 2040
gagatcgagt gggagctgca gaaggagaac agcaaacgct ggaatcccga aattcagtac 2100
acttccaact acaacaaatc tgttaatgtg gactttactg tggacactaa tggtgtgtat 2160
tcagagcctc gccccattgg caccagatac ctgactcgta atctgtaa 2208
<210>67
<211>2208
<212>DNA
<213>新AAV血清型,克隆hu.22
<400>67
atggctgccg atggttatct tccagattgg ctcgaggaca ctctctctga aggaataaga 60
cagtggtgga agctcaaacc tggcccacca ccaccaaagc ccgcagagcg gcataaggac 120
gacagcaggg gtcttgtgct tcctgggtac aagtacctcg gacccttcaa cggactcgac 180
aagggagagc cggtcaacga ggcagacgcc gcggccctcg agcacgacaa ggcctacgac 240
cggcagctcg acagcggaga caacccgtac ctcaagtaca accacgccga cgcggagttt 300
caggagcgcc ttaaaggaga tacgtctttt gggggcaacc tcggacgagc agtcttccag 360
gccaaaaaga ggattcttga acctctgggc ctggttgagg aacctgttaa gacggctccg 420
ggaaaaaaga ggccggtaga gcactctcct gcggagccag actcctcctc gggaaccgga 480
aaagcgggcc agcagcctgc aagaaagaga ttgaattttg gtcagactgg agacgcagac 540
tcagtacctg acccccagcc tctcggacag ccaccagcag ccccctctgg tctgggaact 600
aatacgatgg cttcaggcag tggcgcacca atggcagaca ataacgaggg cgccgacgga 660
gtgggtaatt cctcgggaaa ttggcattgc gattccacat ggatgggcgg cagagtcatc 720
accaccagca cccgcacctg ggccctgccc acctacaaca accatctgta caagcaaatc 780
tccagccagt ctggagccag caacgacaac cactactttg gctacagcac cccctggggg 840
tattttgact tcaacagatt ccactgccac ttctccccac gtgactggca aagactcatc 900
aacaacaact ggggattccg gcccaagaga ctcagcttca agctctttaa cattcaagtc 960
aaagaggtca cgcagaatga cggtacgacg acgattgcca ataaccttac cagcacggtt 1020
caggtgttta ctgactcgga gtaccagctc ccgtacgtcc tcggctcggc gcatcaagga 1080
tgcctcccgc cgttcccagc agacgtcttc atggtgccac agtatggata cctcaccctg 1140
aacaacggca gtcaggcggt aggacgctct tccttttact gcctggagta ctttccttct 1200
cagacgcttc gtaccggaaa caactttacc ttcagctaca cctttgaaga cgttcctttc 1260
catagcagct acgctcacag ccaaagtctg gaccgtctca tgaatcctct catcgaccag 1320
tacctgtatt acttgagcag aacaaacact ccaagcggaa ccaccacgat gtccaggctt 1380
cagttttctc aggccggagc aagtgacatt cgggaccagt ctagaaactg gcttcctgga 1440
ccctgttacc gccagcagcg agtatcaaag acagctgcgg acaacaacaa cagtgattac 1500
tcgtggactg gagctaccaa gtaccacctc aatggaagag actctctggt gaatccgggc 1560
ccagctatgg ccagccacaa ggacgatgaa gaaaaatatt ttcctcagag cggggttctc 1620
atctttggaa aacaagactc gggaaaaact aatgtggaca ttgaaaaggt tatgattaca 1680
gacgaagagg aaatcaggac caccaatccc gtggctacgg agcagtatgg ttctgtatct 1740
accaacctcc agagcggcaa cacacaagca gctacctcag atgtcaacac acaaggcgtt 1800
cttccaggca tggtctggca ggacagagac gtgtacctgc aggggcccat ctgggcaaag 1860
attccacaca cggacggaca ttttcacccc tctcccctca tgggcggatt cggacttaaa 1920
caccctcctc cacaaattct catcaagaac accccggtac ctgcgaatcc ttcgaccact 1980
ttcagtgcgg caaagtttgc ttccttcatc acacagtact ccacggggca ggtcagcgtg 2040
gagatcgagt gggagctgca gaaggagaac agcaaacgct ggaatcccga aattcagtac 2100
acttccaact acaacaaatc tgttaatgtg gactttactg tggacactaa tggtgtgtat 2160
tcagagcctc gccccattgg caccagatac ctgactcgta atctgtaa 2208
<210>68
<211>2208
<212>DNA
<213>新AAV血清型,克隆hu.28
<400>68
atggctgccg atggttatct tccagattgg ctcgaggaca ctctctctga aggaataaga 60
cagtggtgga aactcaaacc tggcccacca ccaccaaagc ccgcagagcg gcataaggac 120
gacagcaggg gtcttgtgct tcctgggtac aagtacctcg gacccttcaa cggactcgac 180
aagggagagc cggtcaacga ggcagacgcc gcggccctcg agcacgacaa ggcctacgac 240
cggcagctcg acagcggaga caacccgtac ctcaagtaca accacgccga cgcggagttt 300
caggagcgcc ttaaagaaga tacgtctttt gggggcaacc tcggacgagc agtcttccag 360
gcaaaaaaga gggttctgga acctctgagc ctggttgagg agcctgttaa gacggctccg 420
ggaaaaaaga ggccggtaga gcactctccc gcagagccag attcctcctc cggaactgga 480
aagtcgggca accagcctgc aagaaagaga ttgaatttcg gtcagactgg agactcagac 540
tccgtacctg acccccagcc tctcggacag ccaccagcag ccccctctgg tctgggaact 600
aatacgatgg ctacaggcag tggcgcacca atggcagaca ataacgaggg cgccgacgga 660
gtgggtaatt cctcgggaaa ttggcattgc gattccacat ggatgggcga cagagtcatc 720
accaccagca cccgaacctg ggccctgccc acctacaaca accatctgta caagcaaata 780
tccagccagt ctggagccag caacgacaat cactactttg gctacagcac cccctggggg 840
tattttgact tcaacagatt ccactgccac ttttcaccac gtgactggca aagactcatc 900
aacaacaact ggggattccg acccaagaga ctcaacttca agctctttaa cattcaagtc 960
aaagaggtca cgcagaatga cggtacgacg acgattgcca ataaccttac cagcacggtt 1020
caggtgttta ctgactcgga gtaccagctc ccgtacgtcc tcggctcggc gcatcaagga 1080
tgcctcccgc cgttcccagc agacgtcttc atggtgccac agtatggata cctcaccctg 1140
aacaacggga gtcaggcagt aggacgctct tcattttact gcctagagta ctttccttct 1200
cagatgctgc gtaccggaaa caactttacc ttcagctaca cctttgagga cgttcctttc 1260
cacagcagct acgctcacag ccagagtttg gaccgtctca tgaatcctct catcgaccag 1320
tacctgtatt acttgagcag aacaaacact ccaagcggaa ccaccacgca gtccaggctt 1380
cagttttctc aggccggagc gagtgacatt caggaccagt ctaggaactg gcttcctgga 1440
ccctgttacc gtcagcagcg agtatcaaag acatctgcgg ataacaacaa cagtgaatac 1500
tcgtggactg gagctaccaa gtaccacctc aatggcagag actctctggt gaatccgggc 1560
ccggccatgg ccagccacaa agacgatgaa gaaaagtttt ttcctcagag cggggttctt 1620
atctttggga agcaaggctc agagaaaaca aatgtggata ttgaaaaggt catgattaca 1680
gacgaagagg aaatcaggac caccaatccc gtggctacgg agcagtatgg ttctgtatct 1740
accaacctcc agagcggcaa cacacaagca gctaccgcag atgtcaacac acaaggcgtt 1800
cttccaggca tggtcgggca agacagagac gtgtacctgc aggggcctac ttgggcaaag 1860
attccacaca cggacggaca ttttcacccc tctcccctca tgggcggatt tggacttaaa 1920
caccctcctc cacagattct catcaagaac accccggtac ctgcgaatcc ttcgaccacc 1980
ttcagtgcgg caaagtttgc ttccttcatt acacagtact ccacggggca ggtcagcgtg 2040
gagatcgagt gggagctgca gaaggagaac agcaaacgct ggaatcccga gatccagtac 2100
acttccaact acaacaagtc tgttaatgtg gactttactg tggacactaa tggcgtgtat 2160
tcagagcctc gccccattgg caccagatac ctgactcgta atctgtaa 2208
<210>69
<211>2208
<212>DNA
<213>新AAV血清型,克隆hu.29
<400>69
atggctgccg atggttatct tccagattgg ctcgaggaca ctctctctga aggaataaga 60
cagtggtgga agctcaaacc tggcccacca ccaccaaagc ccgcagagcg gcataaggac 120
gacagcaggg gtcttgtgct tcctgggtac aagtacctcg gacccttcaa cggactcgac 180
aagggagagc cggtcaacga ggcagacgcc gcggccctcg agcacgacaa ggcctacgac 240
cggcagctcg acagcggaga caacccgtac ctcaagtaca accacgccga cgcagagttt 300
caggagcgcc ttaaagaaga tacgtctttt gggggcaacc tcggacgagc agtcttccag 360
gcaaaaaaga gggttctgga acctctgggc ctggttgagg agcctgttaa gacggctccg 420
ggaaaaaaga ggccggtaga gcactctcct gcagagccag attcctcctc cggaactgga 480
aagtcgggca accagcctgc aagaaagaga ttgaatttcg gtcagactgg agactcagac 540
tccgtacctg acccccagcc tctcggacag ccaccagcag ccccctctgg tctgggaact 600
aatacgatgg ctacaggcag tggcgcacca atggcagaca ataacgaggg cgccgacgga 660
gtgggtaatt cctcgggaaa ttggcattgc gattccacat ggatgggcga cagagtcatc 720
accaccagca cccgaacctg ggccctgccc acctacaaca accatctgta caagcaaata 780
tccagccagt ctggagccag caacgacaat cactactttg gctacagcac cccctggggg 840
tattttgact tcaacagatt ccactgccac ttttcaccac gtgactggca aagactcatc 900
aacaacaact ggggattccg acccaagaga ctcaacttca agctctttaa cattcaagtc 960
aaagaggtca cgcagaatga cggtacgacg acgattgcca ataaccttac cagcacggtt 1020
caggtgttta ctgactcgga gtaccagctc ccgtacgtcc tcggctcggc gcatcaagga 1080
tgcctcccgc cgttcccagc agacgtcttc atggtgccac agtatggata cctcaccctg 1140
aacaacggga gtcaggcagt aggacgctct tcattttact gcctagggta ctttccttct 1200
cagatgctgc gtaccggaaa caactttacc ttcagctaca cctttgagga cgttcctttc 1260
cacagcagct acgctcacag ccagagtttg gaccgtctca tgaatcctct catcgaccag 1320
tacctgtatt acttgagcag aacaaacact ccaagcggaa ccaccacgca gtccaggctt 1380
cagttttctc aggccggagc gagtgacatt cgggaccagt ctaggaactg gcttcctgga 1440
ccctgttacc gtcagcagcg agtatcaaag acatctgcgg ataacaacaa cagtgaatac 1500
tcgtggactg gagctaccaa gtaccacctc aatggcagag actctctggt gaatccgggc 1560
ccggccatgg ccagccacaa agacgatgaa gaaaagtttt ttcctcagag cggggttctt 1620
atctttggga agcaaggccc agagaaaaca aatgtggata ttgaaaaggt catgattaca 1680
gacgaagagg aaatcaggac caccaatccc gtggctacgg agcagtatgg ttctgtatct 1740
accaacctcc agagcggcaa cacacaagca gctaccgcag atgtcaacac acaaggcgtt 1800
cttccaggca tggtctggca agacagagac gtgtacctgc aggggcctat ttgggcaaag 1860
attccacaca cggacggaca ttttcacccc tctcccctca tgggcggatt tggacttaaa 1920
caccctcctc cacagattct catcaagaac accccggtac ctgcgaatcc ttcgaccacc 1980
ttcagtgcgg caaagtttgc ttccttcatt acacagtact ccacggggca ggtcagcgtg 2040
gagatcgagt gggagctgca gaaggagaac agcaaacgct ggaatcccga gatccagtac 2100
acttccaact acaacaagtc tgttaatgtg gactttactg tggacactaa tggcgtgtat 2160
tcagagcctc gccccattgg caccagatac ctgactcgta atctgtaa 2208
<210>70
<211>2208
<212>DNA
<213>新AAV血清型,克隆hu.30
<400>70
atggctgccg atggttatct tccagattgg ctcgaggaca ctctctctga aggaataaga 60
cagtggtgga agctcaaacc tggcccacca ccaccaaagc ccgcagagcg gcataaggac 120
gacagcaggg gtcttgtgct tcctgggtac aagtacctcg gacccttcaa cggactcgac 180
aagggagagc cggtcaacga ggcagacgcc gcggccctcg agcacgacaa ggcctacgac 240
cggcagctcg acagcggaga caacccgtac ctcaagtaca accacgccga cgcggagttt 300
caggagcgcc ttaaagagga tacgtctttt gggggcaacc tcggacgagc agtcttccag 360
gcaaaaaaga gggttctgga acctctgggc ctggttgagg agcctgttaa gacggctccg 420
ggaaaaaaga ggccggtaga gcactctcct gcagagccag attcctcctc cggaactgga 480
aagtcgggca accagcctgc aagaaagaga ttgaatttcg gtcagactgg agactcagac 540
tccgtacctg acccccagcc tctcggacag ccaccagcag ccccctctgg tctgggaact 600
aatacgatgg ctacaggcag tggcgcacca atggcagaca ataacgaggg cgccgacgga 660
gtgggtaatt cctcgggaaa ttggcattgc gattccacat ggatgggcga cagagtcatc 720
accaccagca cccgaacctg ggccctgccc acctacaaca accatctgta caagcaaata 780
tccagccagt ctggagccag caacgacaat cactactttg gctacagcac cccctggggg 840
tattttgact tcaacagatt ccactgccac ttttcaccac gtgactggca aagactcatc 900
aacaacaact ggggattccg acccaagaga ctcaacttca agctctttaa cattcaagtc 960
aaagaggtca cgcagaatga cggtacgacg acgattgcca ataaccttac cagcacggtt 1020
caggtgttta ctgactcgga gtaccagctc ccgtacgtcc tcggctcggc gcatcaagga 1080
tgcctcccgc cgttcccagc agacgtcttc atggtgccac agtatggata cctcaccctg 1140
aacaacggga gtcaggcagt aggacgctct tcattttact gcctagagta ctttccttct 1200
cagatgctgc gtaccggaaa cagctttacc ttcagctaca cctttgagga cgttcctttc 1260
cacagcagct acgctcacag ccagagtttg gaccgtctca tgaatcctct catcgaccag 1320
tacctgtatt acttgagcag aacaaacact ccaagcggaa ccaccacgca gtccaggctt 1380
cagttttctc aggccggagc gagtgacatt cgggaccagt ctaggaactg gcttcctgga 1440
ccctgttacc gtcagcagcg agtatcaaag acatctgcgg ataacaacaa cagtgaatac 1500
tcgtggactg gagctaccaa gtaccacctc aatggcagag actctctggt gaatccgggc 1560
ccggccatgg ccagccacaa agacgatgaa gaaaagttct ttcctcagag cggggttctt 1620
atctttggga agcaaggctc agagaaaaca aatgtggata ttgaaaaggt catgattaca 1680
gacgaagagg aaatcaggac caccaatccc gtggctacgg agcagtatgg ttctgtatct 1740
accaacctcc agagcggcaa cacacaagca gctaccgcag atgtcaacac acaaggcgtt 1800
cttccaggca tggtctggca agacagagac gtgtacctgc aggggcctat ttgggcaaag 1860
attccacaca cggacggaca ttttcacccc tctcccctca tgggcggatt tggacttaaa 1920
caccctcctc cacagattct catcaagaac accccggtac ctgcgaatcc ttcgaccacc 1980
ttcagtgcgg caaagtttgc ttccttcatt acacagtact ccacggggca ggtcagcgtg 2040
gagatcgagt gggagctgca gaaggagaac agcaaacgct ggaatcccga gatccagtac 2100
acttccaact acaacaagtc tgttaatgtg gactttactg tggacactaa tggcgtgtat 2160
tcagagcctc gccccattgg caccagatac ctgactcgta atctgtaa 2208
<210>71
<211>2208
<212>DNA
<213>新AAV血清型,克隆hu.13
<400>71
atggctgccg atggttatct tccagattgg ctcgaggaca ctctctctga aggaataaga 60
cagtggtgga agctcaaacc tggcccacca ccaccaaagc ccgcagagcg gcataaggac 120
gacagcaggg gtcttgtgct tcctgggtac aagtacctcg gacccttcaa cggactcgac 180
aagggagagc cggtcaacga ggcagacgcc gcggccctcg agcacgacaa ggcctacgac 240
cggcagctcg acagcggaga caacccgtac ctcaagtaca accacgccga cgcggagttt 300
caggagcgcc ttaaagaaga tacgtctttt gggggcaacc tcggacgagc agtcttccag 360
gcaaaaaaga gggttcttga acctctgggc ctggttgagg agcctgttaa aacggctccg 420
ggaaaaaaga ggccggtaga gcactctcct gcggagccag actcctcctc gggaaccgga 480
aaagcgggcc agcagcctgc aagaaaaaga ttgaatttcg gtcagactgg agacgcagac 540
tccgtacctg acccccagcc tctcggacag ccaccagcag ccccctctgg tctgggaact 600
aatacgatgg cttcaggcag tggcgcacca atggcagaca ataacgaggg cgccgacgga 660
gtgggtaatt cctcgggaaa ttggcattgc gattccacat ggatgggcga cagagtcatc 720
accaccagca cccgaacttg ggccctgccc acctacaaca accatctcta caagcaaatc 780
tccagccaat caggagccag caacgacaac cactactttg gctacagcac cccttggggg 840
tattttgact tcaacagatt ccactgccac ttttcaccac gtgactggca aagactcatc 900
aacaacaact ggggattccg gcccaagaga ctcaacttca agctctttaa cattcaagtc 960
aaagaggtca cgcagaatga cggtacgacg acgattgcca ataaccttac cagcacggtt 1020
caggtgttta ctgactcgga gtaccagctc ccgtacgtcc tcggctcggc gcatcaagga 1080
tgcctcccgc cgttcccagc agacgtcttc atggtgccac agtatggata cctcaccctg 1140
aacaacggga gtcaggcagt aggacgctct tcattttact gcctggagta ctttccttct 1200
cagatgctgc gtaccggaaa caactttacc ttcagctaca cctttgagga cgttcctttc 1260
cacagcagct acgctcacag ccagagtttg gaccgtctca tgaatcctct catcgaccag 1320
tacctgtatt acttgagcag aacaaacact ccaagcggaa ccaccacgca gtccaggctt 1380
cagttttctc aggccggagc aagtgacatt cgggaccagt ctaggaactg gcttcctgga 1440
ccctgttacc gccagcagcg agtatcaaag acatctgcgg ataacaacaa cagtgaatac 1500
tcgtggactg gagctaccaa gtaccacctc aatggcagag actctctggt gaatccgggc 1560
ccggccatgg ccagccacaa ggacgatgaa gaaaagtttt ttcctcagag cggggttctc 1620
atctttggga agcaaggctc agagaaaaca aatgtggaca ttgaaaaggt catgattaca 1680
gacgaagagg aaatcaggac caccaatccc gtggctacgg agcagtatgg ttctgtatct 1740
accaacctgc agggcggcaa cacacaagca gctaccgcag atgtcaacac acaaggcgtt 1800
cttccaggca tggtctggca ggacagagac gtgtacctgc aggggcccat ctgggcaaag 1860
attccacaca cggacggaca ttttcacccc tctcccctca tgggcggatt cggacttaaa 1920
caccctcctc cacagattct catcaagaac accccggtac ctgcgaatcc ttcgaccacc 1980
ttcagtgcgg caaagtttgc ttctttcatc acacagtatt ccacggggca ggtcagcgtg 2040
gagatcgagt gggagctgca gaaggagaac agcaaacgct ggaatcccga gatccagtac 2100
acttccaact acaacaaatc tgttaatgtg gactttactg ttgacactaa tggcgtgtat 2160
tcagagcctc gccccattgg caccagatac ctgactcgta atctgtaa 2208
<210>72
<211>2208
<212>DNA
<213>新AAV血清型,克隆hu.34
<400>72
atggctgccg atggttatct tccagattgg ctcgaggaca ctctctctga aggaataaga 60
cagcggtgga agctcaaacc tggcccacca ccaccagagc ccgcagagcg gcataaggac 120
gacagcaggg gtcttgtgct tcctgggtac aagtacctcg gacccttcaa cggactcgac 180
aagggagagc cggtcaacga ggcagacgcc gcggccctcg agcacgacaa agcctacgac 240
cggcagctcg acagcggaga caacccgtac ctcaagtaca accacgccga cgcggagttt 300
caggagcgcc ttaaagaaga tacgtccttt gggggcaacc tcggacgagc agtcttccag 360
gcgaaaaaga gggtacttga acctctgggc ctggttgagg aacctgttaa gacggctccg 420
ggaaaaaaga ggccggtaga gcactctcct gtggagccag actcctcctc gggaaccgga 480
aaggcgggcc agcagcctgc aagaaaaaga ttgaattttg gtcagactgg agacgcagac 540
tcagtacctg acccccagcc tctcggacag ccaccagcag ccccctctgg tctgggaact 600
aatacgatgg ctacaggcag tggcgcacca atggcagaca ataacgaggg cgccgacgga 660
gtgggtaatt cctcgggaaa ttggcattgc gattccacat ggatgggcga cagagtcatc 720
accaccagca cccgaacctg ggccctgccc acctacaaca accacctcta caaacaaatt 780
tccagccaat caggagcctc gaacgacaat cactactttg gctacagcac cccttggggg 840
tattttgact tcaacagatt ccactgccac ttttcaccac gtgactggca aagactcatc 900
aacaacaact ggggattccg acccaagaga ctcaacttca agctctttaa cattcaagtc 960
aaagaggtca cgcagaatga cggtacgacg acgattgcca ataaccttac cagcacggtt 1020
caggtgttta ctgactcgga gtaccagctc ccgtacgtcc tcggctcggc gcatcaagga 1080
tgcctcccgc cgttcccagc agacgtcttc atggtgccac agtatggata cctcaccctg 1140
aacaacgaga gtcaggcagt aggacgctct tcattttact gcctggagta ctttccttct 1200
cagatgctgc gtaccggaaa caactttacc ttcagctaca cttttgagga cgttcctttc 1260
cacagcagct acgctcacag ccagagtctg ggccgtctca tgaatcctct catcgaccag 1320
tacctgtatt acttgagcag aacaaacact ccaagtggaa ccaccacgca gtcaaggctt 1380
cagttttctc aggccggagc gagtgacatt cgggaccagt ctaggaactg gcttcctgga 1440
ccctgttacc gccagcagcg agtatcaaag acatctgcgg ataacaacaa cagtgaatac 1500
tcgtggactg gagctaccaa gtaccacctc aatggcagag actctctggt gaatccgggc 1560
ccggccatgg caagccacaa ggacgatgaa gaaaagtttt ttcctcagag cggggttctc 1620
atctttggga agcaaggctc agagaaaaca aatgtggaca ttgaaaaggt catgattaca 1680
gacgaagagg aaatcaggac aaccaatccc gtggctacgg agcagtatgg ttctgtatct 1740
accaacctcc agagaggcaa cagacaagca gctaccgcag atgtcaacac acaaggcgtt 1800
cttccaggca tggtctggca ggacagagat gtgtaccttc aggggcccat ctgggcaaag 1860
attccacaca cggacggaca ttttcacccc tctcccctca tgggtggatt cggacttaaa 1920
caccctcctc cacagattct catcaagaac accccggtac ctgcgaatcc ttcgaccacc 1980
ttcagtgcgg caaagtttgc ttccttcatc acacagtact ccacgggaca ggtcagcgtg 2040
gagatcgagt gggagctgca gaaggaaaac agcaaacgct ggaatcccga aattcagtac 2100
acttccaact acaacaagtc tgttaatgtg gactttactg tggacactaa tggcgtgtat 2160
tcagagcctc gccccattgg caccagatac ctgactcgta atctgtaa 2208
<210>73
<211>2208
<212>DNA
<213>新AAV血清型,克隆hu.35
<400>73
atggctgccg atggttatct tccagattgg ctcgaggaca ctctctctga aggaataaga 60
cagcggtgga agctcaaacc tggcccacca ccaccagagc ccgcagagcg gcataaggac 120
gacagcaggg gtcttgtgct tcctgggtac aagtacctcg gacccttcaa cggactcgac 180
aagggagagc cggtcaacga ggcagacgcc gcggccctcg agcacgacaa agcctacgac 240
cggcagctcg acagcggaga caacccgtac ctcaagtaca accacgccga cgcggagttt 300
caggagcgcc ttaaagaaga tacgtctttt gggggcaacc tcggacgagc agtcttccag 360
gcgaaaaaga gggtacttga acctctgggc ctggttgagg aacctgttaa gacggctccg 420
ggaaaaaaga ggccggtaga gcactctcct gtggagccag actcctcctc gggaaccgga 480
aaggcgggcc agcagcctgc aagaaaaaga ttgaattttg gtcagactgg agacgcagac 540
tcagtacctg acccccagcc tctcggacag ccaccagcag ccccctctgg tctgggaact 600
aatacgatgg ctacaggcag tggcgcacca atggcagaca ataacgaggg cgccgacgga 660
gtgggtaatt cctcgggaaa ttggcattgc gattccacat ggatgggcga cagagtcatc 720
accaccagca cccgaacctg ggccctgccc acctacaaca accacctcta caaacaaatt 780
tccagccaat caggagcctc gaacgacaat cactactttg gctacagcac cccttggggg 840
tattttgact tcaacagatt ccactgccac ttttcaccac gtgactggca aagactcatc 900
aacaacaact ggggattccg acccaagaga ctcaacttca agctctttaa cattcaagtc 960
aaagaggtca cgcagaatga cggtacgacg acgattgcca ataaccttac cagcacggtt 1020
caggtgttta ctgactcgga gtaccagctc ccgtacgtcc tcggctcggc gcatcaagga 1080
tgcctcccgc cgttcccagc agacgtcttc atggtaccac agtatggata cctcaccctg 1140
aacaacggga gtcaggcagt aggacgctct tcattttact gcctggagta ctttccttct 1200
cagatgctgc gtaccggaaa caactttacc ttcagctaca cttttgagga cgttcctttc 1260
cacagcagct acgctcacag ccagagtctg ggccgtctca tgaatcctct catcgaccag 1320
tacctgtatt acttgagcag aacaaacact ccaagtggaa ccaccacgca gtcaaggctt 1380
cagttttctc aggccggagc gagtgacatt cgggaccagt ctaggaactg gcttcctgga 1440
ccctgttacc gccagcagcg agtatcaaag acatctgcgg ataacaacaa cagtgaatac 1500
tcgtggactg gagctaccaa gtaccacctc aatggcagag actctctggt gaatccgggc 1560
ccggccatgg caagccacaa ggacgatgaa gaaaagtttt ttcctcagag cggggttctc 1620
atctttggga agcaaggctc agagaaaaca aatgtggaca ttgaaaaggt catgattaca 1680
gacgaagagg aaatcaggac aaccaatccc gtggctacgg agcagtatgg ttctgtatct 1740
accaacctcc agagaggcaa cagacaagca gctaccgcag atgtcaacac acaaggcgtt 1800
cttccaggca tggtctggca ggacagagat gtgtaccttc aggggcccat ctgggcaaag 1860
attccacaca cggacggaca ttttcacccc tctcccctca tgggtggatt cggacttaaa 1920
caccctcctc cacagattct catcaagaac accccggtac ctgcgaatcc ttcgaccacc 1980
ttcagtgcgg caaagtttgc ttccttcatc acacagtact ccacgggaca ggtcagcgtg 2040
gagatcgagt gggagctgca gaaggaaaac agcaaacgct ggaatcccga aattcagtac 2100
acttccaact acaacaagtc tgttaatgtg gactttactg tggacactaa tggcgtgtat 2160
tcagagcctc gccccattgg caccagatac ctgactcgta atctgtaa 2208
<210>74
<211>2208
<212>DNA
<213>新AAV血清型,克隆hu.36
<400>74
atggctgccg atggttatct tccagattgg ctcgaggaca ctctctctga aggaataaga 60
cagcggtgga agctcaaacc tggcccacca ccaccagagc ccgcagagcg gcataaggac 120
gacagcaggg gtcttgtgct tcctgggtac aagtacctcg gacccttcaa cggactcgac 180
aagggagagc cggtcaacga ggcagacgcc gcggccctcg agcacgacaa agcctacgac 240
cggcagctcg acagcggaga caacccgtac ctcaagtaca accacgccga cgcggagttt 300
caggagcgcc ttaaagaaga tacgtctttt gggggcaacc tcggacgagc agtcttccag 360
gcgaaaaaga gggtactcga acctctgggc ctggttgagg aacctgttaa gacggctccg 420
ggaaaaaaga ggccggtaga gcactctcct gtggagccag actcctcctc gggaaccgga 480
aaggcgggcc agcagcctgc aagaaaaaga ttgaattttg gtcagactgg agacgcagac 540
tcagtacctg acccccagcc tctcggacag ccaccagcag ccccctctgg tctgggaact 600
aatacgatgg ctacaggcag tggcgcacca atggcagaca ataacgaggg cgccgacgga 660
gtgggtaatt cctcgggaaa ttggcattgc gattccacat ggatgggcga cagagtcatc 720
accaccagca cccgaacctg ggccctgccc acctacaaca accacctcta caaacaaatt 780
tccagccaat caggagcctc gaacgacaat cactactttg gctacagcac cccttggggg 840
tattttgact tcaacagatt ccactgccac ttttcaccac gtgactggca aagactcatc 900
aacaacaact ggggattccg acccaagaga ctcaacttca agctctttaa cattcaagtc 960
aaagaggtca cgcagaatga cggtacgacg acgattgcca ataaccttac cagcacggtt 1020
caggtgttta ctgactcgga gtaccagctc ccgtacgtcc tcggctcggc gcatcaagga 1080
tgcctcccgc cgttcccagc agacgtcttc atggtgccac agtatggata cctcaccctg 1140
aacaacggga gtcaggcagc aggacgctct tcattttact gcctggagta ctttccttct 1200
cagatgctgc gtaccggaaa caactttacc ttcagctaca cttttgagga cgttcctttc 1260
cacagcagct acgctcacag ccagagtctg ggccgtctca tgaatcctct catcgaccag 1320
tacctgtatt acttgagcag aacaaacact ccaagtggaa ccaccacgca gtcaaggctt 1380
cagttttctc aggccggagc gagtgacatt cgggaccagt ctaggaactg gcttcctgga 1440
ccctgttacc gccagcagcg agtatcaaag acatctgcgg ataacaacaa cagtgaatac 1500
tcgtggactg gagctaccaa gtaccacctc aatggcagag actctctggt gaatccgggc 1560
ccggccatgg caagccacaa ggacgatgaa gaaaagtttt ttcctcagag cggggttctc 1620
atctttggga agcaaggctc agagaaaaca aatgtggaca ttgaaaaggt catgattaca 1680
gacgaagagg aaatcaggac aaccaatccc gtggctacgg agcagtatgg ttctgtatct 1740
accaacctcc agagaggcaa cagacaagca gctaccgcag atgtcaacac acaaggcgtt 1800
cttccaggca tggtctggca ggacagagat gtgtaccttc aggggcccat ctgggcaaag 1860
attccacaca cggacggaca ttttcacccc tctcccctca tgggtggatt cggacttaaa 1920
caccctcctc cacagattct catcaagaac accccggtac ctgcgaatcc ttcgaccacc 1980
ttcagtgcgg caaagtttgc ttccttcatc acacagtact ccacgggaca ggtcagcgtg 2040
gagatcgagt gggagctgca gaaggaaaac agcaaacgct ggaatcccga aattcagtac 2100
acttccaact acaacaagtc cgttaatgtg gactttactg tggacactaa tggcgtgtat 2160
tcagagcctc gccccattgg caccagatac ctgactcgta atctgtaa 2208
<210>75
<211>2208
<212>DNA
<213>新AAV血清型,克隆hu.33
<400>75
atggctgccg atggttatct tccagattgg ctcgaggaca ctctctctga aggaataaga 60
cagcggtgga agctcaaacc tggcccacca ccaccagagc ccgcagagcg gcataaggac 120
gacagcaggg gtcttgtgct tcctgggtac aagtacctcg gacccttcaa cggactcgac 180
aagggagagc cggtcaacga ggcagacgcc gcggccctcg agcacgacaa agcctacgac 240
cggcagctcg acagcggaga caacccgtac ctcaagtaca accacgccga cgcggagttt 300
caggagcgcc ttaaagaaga tacgtctttt gggggcaacc tcggacgagc agtcttccag 360
gcgaaaaaga gggtacttga acctctgggc ctggttgagg aacctgttaa gacggctccg 420
ggaaaaaaga ggccggtaga gcactctcct gtggagccag actcctcctc gggaaccgga 480
aaggcgggcc agcagcctgc aagaaaaaga ttgaattttg gtcagactgg agacgcagac 540
tcagtacctg acccccagcc tctcggacag ccaccagcag ccccctctgg tctgggaact 600
aatacgatgg ctacaggcag tggcgcacca atggcagaca ataacgaggg cgccgacgga 660
gtgggtaatt cctcgggaaa ttggcattgc gattccacat ggatgggcga cagagtcatc 720
accaccagca cccgaacctg ggccctgccc acctacaaca accacctcta caaacaaatt 780
tccagccaat caggagcctc gaacgacaat cactactttg gctacagcac cccttggggg 840
tattttgact tcaacagatt ccactgccac ttttcaccac gtgactggca aagactcatc 900
aacaacaact ggggattccg acccaagaga ctcaacttca agctctttaa cattcaagtc 960
aaagaggtca cgcagaatga cggtacgacg acgattgcca ataaccttac cggcacggtt 1020
caggtgttta ctgactcgga gtaccagctc ccgtacgtcc tcggctcggc gcatcaagga 1080
tgcctcccgc cgttcccagc agacgtcttc atggtgccac agtatggata cctcaccctg 1140
aacaacggga gtcaggcagt aggacgctct tcattttact gcctggagta ctttccttct 1200
cagatgctgc gtaccggaaa caactttacc ttcagctaca cttttgagga cgttcctttc 1260
cacagcagct acgctcacag ccagagtctg ggccgtctca tgaatcctct catcgaccag 1320
tacctgtatt acttgagcag aacaaacact ccaagtggaa ccaccacgca gtcaaggctt 1380
cagttttctc aggccggagc gagtgacatt cgggaccagt ctaggaactg gcttcctgga 1440
ccctgttacc gccagcagcg agtatcaaag acatctgcgg ataacaacaa cagtgaatac 1500
tcgtggactg gagctaccaa gtaccacctc aatggcagag actctctggt gaatccgggc 1560
ccggccatgg caagccacaa ggacgatgaa gaaaagtttt ttcctcagag cggggttctc 1620
atctttggga agcaaggctc agagaaaaca aatgtggaca ttgaaaaggt catgattaca 1680
gacgaagagg aaatcaggac aaccaatccc gtggctacgg agcagtatgg ttctgtatct 1740
accaacctcc agagaggcaa cagacaagca gctaccgcag atgtcaacac acaaggcgtt 1800
cttccaggca tggtctggca ggacagagat gtgtaccttc aggggcccat ctgggcaaag 1860
attccacaca cggacggaca ttttcacccc tctcccctca tgggtggatt cggacttaaa 1920
caccctcctc cacagattct catcaagaac accccggtac ctgcgaatcc ttcgaccacc 1980
ttcagtgcgg caaagtttgc ttccttcatc acacagtact ccacgggaca ggtcagcgtg 2040
gagatcgagt gggagctgca gaaggaaaac agcaaacgct ggaatcccga aattcagtac 2100
acttccaact acaacaagtc tgttaatgtg gactttactg tggacactaa tggcgtgtat 2160
tcagagcctc gccccattgg caccagatac ctgactcgta atctgtaa 2208
<210>76
<211>2208
<212>DNA
<213>新AAV血清型,克隆hu.45
<400>76
atggctgccg atggctatct tccagattgg ctcgaggaca ctctctctga aggaataaga 60
cagtggtgga agctcaaacc tggcccacca ccaccaaagc ccgcagagcg gcatagggac 120
gacagcaggg gtcttgtgct tcctgggtac aagtacctcg gacccttcaa cggactcgac 180
aagggagagc cggtcaacga ggcagacgcc gcggccctcg agcacgacaa agcctacgac 240
cggcagctcg acagcggaga caacccgtac ctcaagtaca accacgccga cgcggagttt 300
caggagcgcc ttaaagaaga tacgtctttt gggggcaacc tcggacgagc agtcttccag 360
gcgaaaaaga gggttcttga acctctgggc ctggttgagg aacctgttaa gacggctccg 420
ggaaaaaaga ggccggtaga gcactctcct gtggagccag actcctcctc gggaaccgga 480
aaggcgggcc agcagcctgc aagaaaaaga ttgaattttg gtcagactgg agacgcagac 540
tcagtacctg acccccagcc tctcggacag ccaccagcag ccccctctgg tctgggaact 600
aatacgatgg ctacaggcag tggcgcacca atggcagaca ataacgaggg cgccgacgga 660
gtgggtaatt cctcgggaaa ttggcattgc gattccacat ggatgggcga cagagtcatc 720
accaccagca cccgaacctg ggccctgccc acctacaaca accacctcta caaacaaatt 780
tccagccaat caggagcctc gaacgacaat cactactttg gctacagcac cccttggggg 840
tattttgact tcaacagatt ccactgccac ttttcaccac gtgactggca aagactcatc 900
aacaacaact ggggattccg acccaagaga ctcaacttca agctctttaa cattcaagtc 960
aaagaggtca cgcagaatga cggtacgacg acgattgcca ataaccttac cagcacggtt 1020
caggtgttta ctgactcggg gtaccagctc ccgtacgtcc tcggctcggc gcatcaagga 1080
tgcctcccgc cgttcccagc agacgtcttc atggtgccac agtatggata ccccaccctg 1140
aacaacggga gtcaggcagt aggacgctct tcattttact gcctggagta ctttccttct 1200
cagatgctgc gtaccggaaa caactttacc ttcagctaca cttttgagga cgttcctttc 1260
cacagcagct acgctcacag ccagagtctg gaccgtctca tgaatcctct catcgaccag 1320
tacctgtatt acttgagcac aacaaacact ccaagtggaa ccaccacgca gtcaaggctt 1380
cagttttctc aggccggagc gagtgacatt cgggaccagt ctaggaactg gcttcctgga 1440
ccctgttacc gccagcagcg agtatcaaag acatctgcgg ataacaacaa cagtgaatac 1500
tcgtggactg gagctaccaa gtaccacctc aatggcagag actctctggt gaatccgggc 1560
ccggccgtgg caagccacaa ggacgatgaa gaaaagtttt ttcctcagag cggggttctc 1620
atctttggga agcaaggctc agagaaaaca aatgtggaca ttgaaaaggt catgattaca 1680
gacgaagagg aaatcaggac aaccaatccc gtggctacgg agcagtatgg ttctgtatct 1740
accaacctcc agagaggcaa cagacaagca gctaccgcag atgtcaacac acaaggcgtt 1800
cttccaggca tggtctggca ggacagagat gtgtaccttc aggggcccat ctgggcaaag 1860
attccacaca cggacggaca ttttcacccc tctcccctca tgggtggatt cggacttaaa 1920
caccctcctc cacagattct catcaagaac accccggtac ctgcgaatcc ttcgaccacc 1980
ttcagtgcgg caaagtttgc ttccttcatc acacagtact ccacgggaca ggtcagcgtg 2040
gagatcgagt gggagctgca gaaggaaaac agcaaacgct ggaatcccga aattcagtac 2100
acttccaact acaacaagtc tgttaatgtg gactttactg tggacactaa tggcgtgtat 2160
tcagagcctc gccccattgg caccagatac ctgactcgta atctgtaa 2208
<210>77
<211>2208
<212>DNA
<213>新AAV血清型,克隆hu.47
<400>77
atggctgccg atggctatct tccagattgg ctcgaggaca ctctctctga aggaataaga 60
cagtggtgga agctcaaacc tggcccacca ccaccaaagc ccgcagagcg gcatagggac 120
gacagcaggg gtcttgtgct tcctgggtac aagtacctcg gacccttcaa cggactcgac 180
aagggagagc cggtcaacga ggcagacgcc gcggccctcg agcacgacaa agcctacgac 240
cggcagctcg acagcggaga caacccgtac ctcaagtaca accacgccga cgcggagttt 300
caggagcgcc ttaaagaaga tacgtctttt gggggcaacc tcggacgagc agtcttccag 360
gcgaaaaaga gggttcttga acctctgggc ctggttgggg aacctgttaa gacggctccg 420
ggaaaaaaga ggccggtaga gcactctcct gtggagccag actcctcctc gggaaccgga 480
aaggcgggcc agcagcctgc aagaaaaaga ttgaattttg gtcagactgg agacgcagac 540
tcagtacctg acccccagcc tctcggacag ccaccagcag ccccctctgg tctgggaact 600
aatacgatgg ctacaggcag tggcgcacca atggcagaca ataacgaggg cgccgacgga 660
gtgggtaatt cctcgggaaa ttggcattgc gattccacat ggatgggcga cagagtcatc 720
accaccagca cccgaacctg ggccctgccc acctacaaca accacctcta caaacaaatt 780
tccagccaat caggagcctc gaacgacagt cactactttg gctacagcac cccttggggg 840
tattttgact tcaacagatt ccactgccac ttttcaccac gtgactggca aagactcatc 900
aacaacaact ggggattccg acccaagaga ctcaacttca agctctttaa cattcaagtc 960
aaagaggtca cgcagaatga cggtacgacg acgattgcca ataaccttac cagcacggtt 1020
caggtgttta ctgactcgga gtaccagctc ccgtacgtcc tcggctcggc gcatcaagga 1080
tgcctcccgc cgttcccagc agacgtcttc atggtgccac agtatggata cctcaccctg 1140
aacaacggga gtcaggcagt aggacgctct tcattttact gcctggagta ctttccttct 1200
cagatgctgc gtaccggaaa caactttacc ttcagctaca cttttgagga cgttcctttc 1260
cacagcagct acgctcacag ccagagtctg gaccgtctca tgaatcctct catcgaccag 1320
tacctgtatt acttgagcac aacaaacact ccaagtggaa ccaccacgca gtcaaggctc 1380
cagttttctc aggccggagc gagtgacatt cgggaccagt ctaggaactg gcttcctgga 1440
ccctgttacc gccagcagcg agtatcaaag acatctgcgg ataacaacaa cagtgaatac 1500
tcgtggactg gagctaccaa gtaccacctc aatggcagag actctctggt gaatccgggc 1560
ccggccatgg caagccacaa ggacaatgaa gaaaagtttt ttcctcagag cggggttctc 1620
atctttggga agcaaggctc agagaaaaca aatgtggaca ttgaaaaggt catgattaca 1680
gacgaagagg aaatcaggac aaccaatccc gtggctacgg agcagtatgg ttctgtatct 1740
accaacctcc agagaggcaa cagacaagca gctaccgcag atgtcaacac acaaggcgtt 1800
cttccaggca tggtctggca ggacagagat gtgtaccttc aggggcccat ctgggcaaag 1860
attccacaca cggacggaca ttttcacccc tctcccctca tgggtggatt cggacttaaa 1920
caccctcctc cacagattct catcaagaac accccggtac ctgcgaatcc ttcgaccacc 1980
ttcagtgcgg caaagtttgc ttccttcatc acacagtact ccacgggaca ggtcagcgtg 2040
gagatcgagt gggagctgca gaaggaaaac agcaaacgct ggaatcccga aattcagtac 2100
acttccaact acaacaagtc tgttaatgtg gactttactg tggacactaa tggcgtgtat 2160
tcagagcctc gccccattgg caccagatac ctgactcgta atctgtaa 2208
<210>78
<211>2211
<212>DNA
<213>新AAV血清型,克隆hu.48
<400>78
atggctgccg atggttatct tccagattgg ctcgaggaca acctctctga gggcattcgc 60
gagtggtggg acttgaaacc tggagccccg aagcccaaag ccaaccagca aaagcaggac 120
gacggccggg gtctggtgct tcctggctac aagtacctcg gacccttcaa cggactcgac 180
aagggggagc ccgtcaacgc ggcggacgca gcggccctcg agcacgacaa ggcctacgac 240
cagcagctca aagcgggtga caatccgtac ctgcggtata accacgccga cgccgagttt 300
caggagcgtc tgcaagaaga tacgtctttt gggggcaacc tcgggcgagc agtcttccag 360
gccaagaagc gggttctcga acctctcggt ctggttgagg aaggcgctaa gacggctcct 420
ggaaagaaac gtccggtaga gcagtcgcca caagagccag actcctcctc gggcatcggc 480
aagacaggcc agcagcccgc taaaaagaga ctcaattttg gccagactgg cgactcagag 540
tcagtccccg atccacaacc tctcggagaa cctccagcaa cccccgctgc tgtgggacct 600
actacaatgg cttcaggcgg tggcgcacca atggcagaca ataacgaagg cgccgacgga 660
gtgggtaatg cctcaggaaa ttggcattgc gattccacat ggctgggcga cagagtcatc 720
accaccagca cccgcacctg ggccttgccc acctacaata accacctcta caagcaaatc 780
tccagtactt caacgggggc cagcaacgac aaccactact tcggctacgg caccccctgg 840
gggtattttg atttcaacag attccactgc cacttttcac cacgtgactg gcagcgactc 900
atcaacaaca attggggatt ccggcccaag agactcaact tcaaactctt caacatccaa 960
gtcgaggagg tcacgacgaa tgatggcgtc acaaccatcg ctaataacct taccagcacg 1020
gttcaagtct tctcggactc ggagtaccag cttccgtacg tcctcggctc tgcgcaccag 1080
ggctgcctcc ctccgttccc ggcggacgtg ttcatgattc cgcaatacgg ctacctgacg 1140
ctcaacaatg gcagccaagc cgtgggacgt tcatcctttt actgcctgga atatttccct 1200
tctcagatgc tgagaacggg caacaacttt accttcagct acacctttga ggaagtgcct 1260
ttccacagca gctacgcgca cagccagagc ctggaccggc tgatgaatcc tctcatcgac 1320
caatacctgt attacctgaa cagaacacaa aatcagtccg gaagtgccca aaacaaggac 1380
ttgctgttta gccgtgggtc tccagctggc atgtctgttc agcccaaaaa ctggctacct 1440
ggaccctgtt atcggcagca gcgcgtttct aaaacaaaaa cagacaacaa caacagcaat 1500
tttacctgga ctggtgcttc aaaatataac ctcaatgggc gtgaatccat catcaaccct 1560
ggcaccgctg tggcctcaca caaagacgac gaagacaagt tctttcccat gagcggtgtc 1620
atgatttttg gaaaagagag cgccggagct tcaagcactg cattggacaa tgtcatgatt 1680
acagacgaag aggaaattaa agccactaac cctgtggcca ccgaaagatt tgggaccgtg 1740
gcagtcaatt tccagagcag cagcacagac cctgcgaccg gagatgtgca tgctatggga 1800
gcattacctg gcatggtgtg gcaagataga gacgtgtacc tgcagggtcc catttgggcc 1860
aaaattcctc acacagatgg acactttcac ccgtctcctc ttatgggcgg ctttggactc 1920
aagaacccgc ctcctcagat cctcatcaaa aacacgcctg ttcctgcgaa tcctccggcg 1980
gagttttcag ctacaaagtt tgcttcattc atcacccaat actccacagg acaagtgagt 2040
gtggaaattg aatgggagct gcagaaagaa aacagcaagc gctggaatcc cgaagtgcag 2100
tacacatcca attatgcaaa atctgccaac gttgatttta ctgtggacaa caatggactt 2160
tatactgagc ctcgccccat tggcacccgt taccttaccc gtcccctgta a 2211
<210>79
<211>2211
<212>DNA
<213>新AAV血清型,克隆rh.71
<400>79
atggctgccg atggttatct tccagattgg ctcgaggaca acctctctga gggcattcgc 60
gagtggtggg acttgaaacc tggagccccg aagcccaaag ccaaccagca aaagcaggac 120
gacggccggg gtctggtgct tcctggctac aagtacctcg gacccttcaa cggactcgac 180
aagggggagc ccgtcaacgc ggcggacgca gcggccctcg agcacgacaa ggcctacgac 240
cagcagctca aagcgggtga caatccgtac ctgcggtata accacgccga cgccgagttt 300
caggagcgtc tgcaagaaga tacgtctttt gggggcaacc tcgggcgagc agtcttccag 360
gccaagaagc gggttctcga acctctcggt ctggttgagg aaggcgctaa gacggctcct 420
ggaaagaaac gtccggtaga gcagtcgcca caagagccag actcctcctc gggcatcggc 480
aagacaggcc agcagcccgc taaaaagaga ctcaattttg gtcagactgg cgactcagag 540
tcagtccccg atccacaacc tctcggagaa cctccagcaa cccccgctgc tgtgggacct 600
actacaatgg cttcaggcgg tggcgcacca atggcagaca ataacgaagg cgccgacgga 660
gtgggtaatg cctcaggaaa ttggcattgc gattccacat ggctgggcga cagagtcatc 720
accaccagca cccgcacctg ggccttgccc acctacaata accacctcta caagcaaatc 780
tccagtgctt caacgggggc cagcaacgac aaccactact tcggctacag caccccctgg 840
gggtattttg atttcaacag attccactgc cacttttcac cacgtgactg gcagcgactc 900
atcaacaaca attggggatt ccggcccaag agactcaact tcaaactctt caacatccaa 960
gtcaaggagg tcacgacgaa tgatggcgtc acaaccatcg ctaataacct taccagcacg 1020
gttcaagtct tctcggactc ggagtaccag cttccgtacg tcctcggctc tgcgcaccag 1080
ggctgcctcc ctccgttccc ggcggacgtg ttcatgattc cgcaatacgg ctacctgacg 1140
ctcaacaatg gcagccaagc cgtgggacgt tcatcctttt actgcctgga atatttccct 1200
tctcagatgc tgagaacggg caacaacttt accttcagct acacctttga ggaagtgcct 1260
ttccacagca gctacgcgca cagccagagc ctggaccggc tgatgaatcc tctcatcgac 1320
caatacctgt attacctgaa cagaactcaa aatcagtccg gaagtgccca aaacaaggac 1380
ttgctgttta gccgtgggtc tccagctggc atgtctgttc agcccaaaaa ctggctacct 1440
ggaccctgtt atcggcagca gcgcgtttct aaaacaaaaa cagacaacaa caacagcaat 1500
tttacctgga ctggtgcttc aaaatataac ctcaatgggc gtgaatccat catcaaccct 1560
ggcactgcta tggcctcaca caaagacgac gaagacaagt tctttcccat gagcggtgtc 1620
atgatttttg gaaaagagag cgccggagct tcaaacactg cattggacaa tgtcatgatt 1680
acagacgaag aggaaattaa agccactaac cctgtggcca ccgaaagatt tgggaccgtg 1740
gcagtcaatt tccagagcag cagcacagac cctgcgaccg gagatgtgca tgctatggga 1800
gcattacctg gcatggtgtg gcaagataga gacgtgtacc tgcagggtcc catttgggcc 1860
aaaattcctc acacagatgg acactttcac ccgtctcctc ttatgggcgg ctttggactc 1920
aagaacccgc ctcctcagat cctcatcaaa aacacgcctg ttcctgcgaa tcctccggcg 1980
gagttttcag ctacaaagtt tgcttcattc atcacccaat actccacagg acaagtgagt 2040
gtggaaattg aatgggagct gcagaaagaa aacagcaagc gctggaatcc cgaagtgcag 2100
tacacatcca attatgcaaa atctgccaac gttgatttta ctgtggacaa caatggactt 2160
tatactgagc ctcgccccat tggcacccgt taccttaccc gtcccctgta a 2211
<210>80
<211>2214
<212>DNA
<213>新AAV血清型,克隆hu.43
<400>80
atggctgctg acggttatct tccagattgg ctcgaggaca acctctctga gggcattcgc 60
gagtggtggg acctgaaacc tggagccccc aagcccaagg ccaaccagca gaagcaggac 120
gacggccggg gtctggtgct tcctggctac aagtacctcg gacccttcaa cggactcgac 180
aagggggagc ccgtcaacgc ggcggacgca gcggccctcg agcacgacaa ggcctacgac 240
cagcagctca aagcgggtga caatccgtac ccgcggtata accacgccga cgccgagttt 300
caggagcgtc tgcaagaaga tacgcctttt gggggcaacc tcgggcgagc agtcttccag 360
gccaagaagc gggttctcga acctctcggt ctggttgagg aagctgctaa gacggctcct 420
ggaaagaaga gaccggtaga accgtcacct cagcgttccc ccgactcctc cacgggcatc 480
ggcaagaaag gccagcagcc cgctaaaaag agactgaact ttggtcagac tggcgactca 540
gagtcagtcc ccgaccctca accaatcgga gaaccaccag caggcccctc tggtctggga 600
tctggtacaa tggctgcagg cggtggcgct ccaatggcag acaataacga aggcgccgac 660
ggagtgggta atgcctcagg aaattggcat tgcgattcca catggctggg cgacagagtc 720
atcaccacca gcacccgcac ctgggccttg cccacctaca ataaccacct ctacaagcaa 780
atctccagtg cttcaacggg ggccagcaac gacaaccact acttcggcta cagcaccccc 840
tgggggtatt ttgatttcaa cagattccac tgccactttt caccacgtga ctggcagcga 900
ctcatcaaca acaattgggg attccggccc aagagactca acttcaaact cttcaacatc 960
caagtcaagg aggtcacgac gaatgatggc gtcacaacca tcgctaataa ccttaccagc 1020
acggttcaag tcttctcgga ctcggagtac cagcttccgt acgtcctcgg ctctgcgcac 1080
cagggctgcc tccctccgtt cccggcggac gtgttcatga ttccgcaata cggctacctg 1140
acgctcaaca atggcagcca agccgtggga cgttcatcct tttactgcct ggaatatttc 1200
ccttctcaga tgctgagaac gggcaacaac tttaccttca gctacacctt tgaggaagtg 1260
cctctccaca gcagctacgc gcacagccag agcctggacc ggctgatgaa tcctctcatc 1320
gtccaatacc tgtattacct gaacagaact caaaatcagt ccggaagtgc ccaaaacaag 1380
gacttgctgt tcagccgtgg gtctccagct ggcatgtctg ttcagcccaa aaactggcta 1440
cctggaccct gttatcggca gcagcgcgtt tctaaaacaa aaacagacaa caacaacagc 1500
aattttacct ggactggtgc ttcaaaatat aacctcaatg ggcgtgaatc catcatcaac 1560
cctggcactg ctatggcctc acacaaagac gacgaagaca agttctttcc catgagcggt 1620
gtcatgattt ttggaaaaga gagcgccgga gcttcaaaca ctgcattgga caatgtcatg 1680
attacagacg aagaggaaat taaagccact aaccctgtgg ccaccgaaag atttgggacc 1740
gtggcagtca atttccagag cagcagcaca gaccctgcga ccggagatgt gcatgctatg 1800
ggagcattac ctggcatggt gtggcaagat agagacgtgt acctgcaggg tcccatttgg 1860
gccaaaattc ctcacacaga tggacacttt cacccgtctc ctcttatggg cggctttgga 1920
ctcaagaacc cgcctcctca gatcctcatc aaaaacacgc ctgttcctgc gaatcctccg 1980
gcggagtttt cagctacaaa gtttgcttca ttcatcaccc aatactccac aggacaagtg 2040
agtgtggaaa ttgaatggga gctgcagaaa gaaaacagca agcgctggaa tcccgaagtg 2100
cagtacacat ccaattatgc aaaatctgcc agcgttgatt ttactgtgga caacaatgga 2160
ctttatactg agcctcgccc cattggcacc cgttacctta cccgtcccct gtaa 2214
<210>81
<211>2211
<212>DNA
<213>新AAV血清型,克隆hu.44
<400>81
atggctgccg atggttatct tccagattgg ctcgaggaca ctctctctga aggaataaga 60
cagtggtgga agctcagacc tggcccacca ccaccaaagc ccgcagagcg gcataaggac 120
gacagcaggg gtcttgtgct tcctgggtac aagtacctcg gacccttcaa cggactcgac 180
aagggagagc cggtcaacga ggcagacgcc gcggccctcg agcacgacaa agcctacgac 240
cggcagctcg acagcggaga caacccgtac ctcaagtaca accacgccga cgcggagttt 300
caggagcgcc ttaaagaaga tacgtctttt gggggcaacc tcgggcgagc agtcttccag 360
gccaagaagc gggttctcga acctctcggt ctggttgagg aaggcgctga gacggctcct 420
ggaaagaaac gtccggtaga gcagtcgcca caagggccag actcctcctc gggcatcggc 480
aagacaggcc agcagcccgc taaaaagaga ctcaattttg gtcagactgg cgactcagag 540
tcagtccccg atccacaacc tctcggagaa cctccagcaa cccccgctgc tgtgggacct 600
actacaatgg cttcaggcgg tggcgcacca atggcagaca ataacgaagg cgccgacgga 660
gtgggtaatg cctcaggaaa ttggcattgc gattccacat ggctgggcga cagagtcatc 720
accaccagca cccgcacctg ggccttgccc acctacaata accacctcta caagcaaatc 780
tccagtgctt caacgggggc cagcaacgac aaccactact tcggctacag caccccctgg 840
gggtattttg atttcaacag attccactgc cacttttcac cacgtgactg gcagcgactc 900
atcaacaaca attggggatt ccggcccaag agactcaact tcaaactctt caacatccaa 960
gtcaaggagg tcacgacgaa tgatggcgtc acaaccatcg ctaataacct taccagcacg 1020
gttcaagtct tctcggactc ggagtaccag cttccgtacg tcctcggctc tgcgcaccag 1080
ggctgcctcc ctccgttccc ggcggacgtg ttcatgattc cgcaatacgg ctacctgacg 1140
ctcaacaatg gcagccaagc cgtgggacgt tcatcctttt actgcctgga atatttccct 1200
tctcagatgc tgagaacggg caacaacttt accttcagct acacctttga ggaagtgcct 1260
ttccacagca gctacgcgca cagccagagc ctggaccggc tgatgaatcc tctcatcgac 1320
caatacctgt attacccgaa cagaactcaa aatcagtccg gaagtgccca aaacaaggac 1380
ttgctgttta gccgtgggtc tccagctggc atgtctgttc agcccaaaaa ctggctacct 1440
ggaccctgtt atcggcagca gcgcgtttct aaaacaaaaa cagacaacaa caacagcaat 1500
tttacctgga ctggtgcttc aaaatataac ctcaatgggc gtgaatccat catcaaccct 1560
ggcactgcta tggcctcaca caaagacgac gaagacaagt tctttcccat gagcggtgtc 1620
atgatttttg gaaaagagag cgccggagct tcaaacactg cattggacaa tgtcatgatt 1680
acagacgaag aggaaattaa agccactaac cctgtggcca ccgaaagatt tgggaccgtg 1740
gcagtcaatt tccagagcag cagcacagac cctgcgaccg gagatgtgca tgctatggga 1800
gcattacctg gcatggtgtg gcaaggtaga gacgtgtacc tgcagggtcc catttgggcc 1860
aaaattcctc acacagatgg acactttcac ccgtctcctc ttatgggcgg ctttggactc 1920
aagaacccgc ctcctcagat cctcatcaaa aacacgcctg ttcctgcgaa tcctccggcg 1980
gagttttcag ctacaaagtt tgcttcattc atcacccaat actccacagg acaagtgagt 2040
gtggaaattg aatgggagct gcagaaagaa aacagcaagc gctggaatcc cgaagtgcag 2100
tacacatcca attatgcaaa atctgccaac gttgatttta ctgtggacaa caatggactt 2160
tatactgagc ctcgccccat tggcacccgt taccttaccc gtcccctgta a 2211
<210>82
<211>2211
<212>DNA
<213>新AAV血清型,克隆hu.46
<400>82
atggctgccg acggttatct tccagattgg ctcgaggaca ctctctctga aggaataaga 60
cagtggtgga agctcaaacc tggcccacca ccaccaaagc ccgcagagcg gcataaggac 120
gacagcaggg gtcttgtgct tcctgggtac aagtacctcg gacccttcaa cggactcgac 180
aagggagagc cggtcaacga ggcagacgcc gcggccctcg agcacgacaa agcctacgac 240
cggcagctcg acagcggaga caacccgtac ctcaagtaca accacgccga cgcggagttt 300
caggagcgcc ttaaagaaga tacgtctttt gggggcaacc tcgggcgggc agtcttccag 360
gccaagaagc gggttctcga acctctcggt ctggttgagg aaggcgctaa gacggctcct 420
ggaaagaaac gtccggtaga gcagtcgcca caagagccag actccccctc gggcatcggc 480
aagacaggcc agcagcccgc taaaaagaga ctcaattttg gtcagactgg cgactcagag 540
tcagtccccg atccacaacc tctcggagaa cctccagcaa cccccgctgc tgtgggacct 600
actacaatgg cttcaggcgg tggcgcacca atggcagaca ataacgaagg cgccgacgga 660
gtgggtaatg cctcaggaaa ttggcactgc gattccacat ggctgggcga cagagtcatc 720
accaccagca cccgcacctg ggccttgccc acctacaata accacctcta caagcaaatc 780
tccagtgctt caacgggggc cagcaacgac aaccactact tcggctacag caccccctgg 840
gggtattttg atttcaacag attccactgc cacttttcac cacgtgactg gcagcgactc 900
atcaacaaca attggggatt ccggcccaag agactcaact tcaaactctt caacatccaa 960
gtcaaggagg tcacgacgaa tgatggcgtc acaaccatcg ctaataacct taccagcacg 1020
gttcaagtct tctcggactc ggagtaccag cttccgtacg tcctcggctc tgcgcaccag 1080
ggccgcctcc ctccgttccc ggcggacgtg ttcatgattc cgcaatacgg ctacctgacg 1140
ctcaacaatg gcagccaagc cgtgggacgt tcatcctctt actgcctgga atatttccct 1200
tctcagatgc tgagaacggg caacaacttt accttcagct acacctttga ggaagtgcct 1260
ctccacagca gctgcgcgca cagccagagc ctggaccggc tgatgaatcc tctcatcgac 1320
caatacctgt attacctgaa cagaactcaa aatcagtccg gaagtgccca aaacagggac 1380
ttgctgttca gccgtgggtc tccagctggc atgtctgttc agcccaaaaa ctggctacct 1440
ggaccctgtt atcggcagca gcgcgtttct aaaacaaaaa cagacaacaa caacagcaat 1500
tttacctgga ctggtgcttc aaaatataac ctcaatgggc gtgaatccat catcaaccct 1560
ggcactgcta tggcctcaca caaagacgac gaagacaagt tctttcccat gagcggtgtc 1620
atgatttttg gaaaagagag cgccggagct tcaaacactg cattggacaa tgtcatgatt 1680
acagacgaag aggaaattaa agccactaac cctgtggcca ccgaaagatt tgggaccgtg 1740
gcagtcaatt tccagagcag cagcacagac cctgcgaccg gagatgtgca tgctatggga 1800
gcattacctg gcatggtgtg gcaagataga gacgtgtacc tgcagggtcc catttgggcc 1860
aaaattcctc acacagatgg acactttcac ccgtctcctc ttatgggcgg ctttggactc 1920
aagaacccgc ctcctcagat cctcatcaaa aacacgcctg ttcctgcgaa tcctccggcg 1980
gagttttcag ctacaaagtt tgcttcattc atcacccaat actccgcagg acaagtgagt 2040
gtggaaattg aatgggagct gcagaaagaa aacagcaagc gctggaatcc cgaagtgcag 2100
tacacatcca attatgcaaa atctgccaac gttgatttta ctgtggacaa caatggactt 2160
tatactgagc ctcgccccat tggcacccgt taccttaccc gtcccctgta a 2211
<210>83
<211>738
<212>PRT
<213>vp1,克隆hu.17
<400>83
Met Ala Ala Asp Gly Tyr Leu Pro Asp Trp Leu Glu Asp Asn Leu Ser
1 5 10 15
Glu Gly Ile Arg Glu Trp Trp Asp Leu Lys Pro Gly Ala Pro Lys Pro
20 25 30
Lys Ala Asn Gln Gln Lys Gln Asp Asp Gly Arg Gly Leu Val Leu Pro
35 40 45
Gly Cys Lys Tyr Leu Gly Pro Phe Asn Gly Leu Asp Lys Gly Glu Pro
50 55 60
Val Asn Ala Ala Asp Ala Ala Ala Leu Glu His Asp Lys Ala Tyr Asp
65 70 75 80
Gln Gln Leu Lys Ala Gly Asp Asn Pro Tyr Leu Arg Tyr Asn His Ala
85 90 95
Asp Ala Glu Phe Gln Glu Arg Leu Gln Glu Asp Thr Ser Phe Gly Gly
100 105 110
Asn Leu Gly Arg Ala Val Phe Gln Ala Lys Lys Arg Val Leu Glu Pro
115 120 125
Leu Gly Leu Val Glu Glu Gly Ala Lys Thr Ala Pro Gly Lys Lys Arg
130 135 140
Pro Val Glu Pro Ser Pro Gln Arg Ser Pro Asp Ser Ser Thr Gly Ile
145 150 155 160
Gly Lys Thr Gly Gln Gln Pro Ala Lys Lys Arg Leu Asn Phe Gly Gln
165 170 175
Thr Gly Asp Ser Glu Ser Val Pro Asp Pro Gln Pro Ile Gly Glu Pro
180 185 190
Pro Ala Gly Pro Ser Gly Leu Gly Ser Gly Thr Met Ala Ala Gly Gly
195 200 205
Gly Ala Pro Met Ala Asp Asn Asn Glu Gly Ala Asp Gly Val Gly Ser
210 215 220
Ser Ser Gly Asn Trp His Cys Asp Ser Thr Trp Leu Gly Asp Arg Val
225 230 235 240
Ile Thr Thr Ser Thr Arg Thr Trp Ala Leu Pro Thr Tyr Asn Asn His
245 250 255
Leu Tyr Lys Gln Ile Ser Asn Gly Thr Ser Gly Gly Ser Thr Asn Asp
260 265 270
Asn Thr Tyr Phe Gly Tyr Ser Thr Pro Trp Gly Tyr Phe Asp Phe Asn
275 280 285
Arg Phe His Cys His Phe Ser Pro Arg Asp Trp Gln Arg Leu Ile Asn
290 295 300
Asn Asn Trp Gly Phe Arg Pro Lys Arg Leu Asn Phe Lys Leu Phe Asn
305 310 315 320
Ile Gln Val Lys Glu Val Thr Gln Asn Glu Gly Thr Lys Thr Ile Ala
325 330 335
Asn Asn Leu Thr Ser Thr Ile Gln Val Phe Thr Asp Ser Glu Tyr Gln
340 345 350
Leu Pro Tyr Val Leu Gly Ser Ala His Gln Gly Cys Pro Pro Pro Phe
355 360 365
Pro Ala Asp Val Phe Met Ile Pro Gln Tyr Gly Tyr Leu Thr Leu Asn
370 375 380
Asn Gly Ser Gln Ala Val Gly Arg Ser Ser Phe Tyr Cys Leu Glu Tyr
385 390 395 400
Phe Pro Ser Gln Met Arg Arg Thr Gly Asn Asn Phe Glu Phe Ser Tyr
405 410 415
Gln Phe Glu Asp Val Pro Phe His Ser Ser Tyr Ala His Ser Gln Ser
420 425 430
Leu Asp Arg Leu Met Asn Pro Leu Ile Asp Gln Tyr Leu Tyr Tyr Leu
435 440 445
Ser Arg Thr Gln Ser Thr Gly Gly Thr Ala Gly Thr Gln Gln Leu Leu
450 455 460
Phe Ser Gln Ala Gly Pro Asn Asn Met Ser Ala Gln Ala Lys Asn Trp
465 470 475 480
Leu Pro Gly Pro Cys Tyr Arg Gln Gln Arg Val Ser Thr Thr Leu Ser
485 490 495
Gln Asn Asn Asn Ser Asn Phe Ala Trp Thr Gly Ala Thr Lys Tyr His
500 505 510
Leu Asn Gly Arg Asp Ser Leu Val Asn Pro Gly Val Ala Met Ala Thr
515 520 525
His Lys Asp Asp Glu Glu Arg Phe Phe Pro Ser Ser Gly Val Leu Met
530 535 540
Phe Gly Lys Gln Gly Ala Gly Lys Asp Asn Val Asp Tyr Ser Ser Val
545 550 555 560
Met Leu Thr Ser Glu Glu Glu Ile Lys Thr Thr Asn Pro Val Ala Thr
565 570 575
Glu Gln Tyr Gly Val Val Ala Asp Asn Leu Gln Gln Gln Asn Ala Ala
580 585 590
Pro Ile Val Gly Ala Val Asn Ser Gln Gly Ala Leu Pro Gly Met Val
595 600 605
Trp Gln Asn Arg Asp Val Tyr Leu Gln Gly Pro Ile Trp Ala Lys Ile
610 615 620
Pro His Thr Asp Gly Asn Phe His Pro Ser Pro Leu Met Gly Gly Phe
625 630 635 640
Gly Leu Lys His Pro Pro Pro Gln Ile Leu Ile Lys Asn Thr Pro Val
645 650 655
Pro Ala Asp Pro Pro Thr Thr Phe Ser Gln Ala Lys Leu Ala Ser Phe
660 665 670
Ile Thr Gln Tyr Ser Thr Gly Gln Val Ser Val Glu Ile Glu Trp Glu
675 680 685
Leu Gln Lys Glu Asn Ser Lys Arg Trp Asn Pro Glu Ile Gln Tyr Thr
690 695 700
Ser Asn Tyr Asn Lys Ser Val Asn Val Asp Phe Thr Val Asp Thr Asn
705 710 715 720
Gly Val Tyr Ser Glu Pro Arg Pro Ile Gly Thr Arg Tyr Leu Thr Arg
725 730 735
Asn Leu
<210>84
<211>738
<212>PRT
<213>vp1,克隆hu.6
<400>84
Met Ala Ala Asp Gly Tyr Leu Pro Asp Trp Leu Glu Asp Asn Leu Ser
1 5 10 15
Glu Gly Ile Arg Glu Trp Trp Asp Leu Lys Pro Gly Ala Pro Lys Pro
20 25 30
Lys Ala Asn Gln Gln Lys Gln Asp Asp Gly Arg Gly Leu Val Leu Pro
35 40 45
Gly Tyr Lys Tyr Leu Gly Pro Phe Asn Gly Leu Asp Lys Gly Glu Pro
50 55 60
Val Asn Ala Ala Asp Ala Ala Ala Leu Glu His Asp Lys Ala Tyr Asp
65 70 75 80
Gln Gln Leu Lys Ala Gly Asp Asn Pro Tyr Leu Arg Tyr Asn His Ala
85 90 95
Asp Ala Glu Phe Gln Glu Arg Leu Gln Glu Asp Thr Ser Phe Gly Gly
100 105 110
Asn Leu Gly Arg Ala Val Phe Gln Ala Lys Lys Arg Val Leu Glu Pro
115 120 125
Leu Gly Leu Val Glu Glu Gly Ala Lys Thr Ala Pro Gly Lys Lys Arg
130 135 140
Pro Val Glu Pro Ser Pro Gln Arg Ser Pro Asp Ser Ser Thr Gly Ile
145 150 155 160
Gly Lys Thr Gly Gln Gln Pro Ala Lys Lys Arg Leu Asn Phe Gly Gln
165 170 175
Thr Gly Asp Ser Glu Ser Val Pro Asp Pro Gln Pro Ile Gly Glu Pro
180 185 190
Pro Ala Gly Pro Ser Gly Leu Gly Ser Gly Thr Met Ala Ala Gly Gly
195 200 205
Gly Ala Pro Met Ala Asp Asn Asn Glu Gly Ala Asp Gly Val Gly Ser
210 215 220
Ser Ser Gly Asn Trp His Cys Asp Ser Ala Trp Leu Gly Asp Arg Val
225 230 235 240
Ile Thr Thr Ser Thr Arg Pro Trp Ala Leu Pro Thr Tyr Asn Asn His
245 250 255
Leu Tyr Lys Gln Ile Ser Asn Gly Thr Ser Gly Gly Ser Thr Asn Asp
260 265 270
Asn Thr Tyr Phe Gly Tyr Ser Thr Pro Trp Gly Tyr Phe Asp Phe Asn
275 280 285
Arg Phe His Cys His Phe Ser Pro Arg Asp Trp Gln Arg Leu Ile Asn
290 295 300
Asn Asn Trp Gly Phe Arg Pro Lys Arg Leu Asn Phe Lys Leu Phe Asn
305 310 315 320
Ile Gln Val Lys Glu Val Thr Gln Asn Glu Gly Thr Lys Thr Ile Ala
325 330 335
Asn Asn Leu Thr Ser Thr Ile Gln Val Phe Thr Asp Ser Glu Tyr Gln
340 345 350
Leu Pro Tyr Val Leu Gly Ser Ala His Gln Gly Cys Pro Pro Pro Phe
355 360 365
Pro Ala Asp Val Phe Met Ile Pro Gln Tyr Gly Tyr Leu Thr Leu Asn
370 375 380
Asn Gly Ser Gln Ala Val Gly Arg Ser Ser Phe Tyr Cys Leu Glu Tyr
385 390 395 400
Phe Pro Ser Gln Met Arg Arg Thr Gly Asn Asn Phe Glu Phe Ser Tyr
405 410 415
Gln Phe Glu Asp Val Pro Phe His Ser Ser Tyr Ala His Ser Gln Ser
420 425 430
Leu Asp Arg Leu Met Asn Pro Leu Ile Asp Gln Tyr Leu Tyr Tyr Leu
435 440 445
Ser Arg Thr Gln Ser Thr Gly Gly Thr Ala Gly Thr Gln Gln Leu Leu
450 455 460
Phe Ser Gln Ala Gly Pro Asn Asn Met Ser Ala Gln Ala Lys Asn Trp
465 470 475 480
Leu Pro Gly Pro Cys Tyr Arg Gln Gln Arg Val Ser Thr Thr Leu Ser
485 490 495
Gln Asn Asn Asn Ser Asn Phe Ala Trp Thr Gly Ala Thr Lys Tyr His
500 505 510
Leu Asn Gly Arg Asp Ser Leu Val Asn Pro Gly Val Ala Met Ala Thr
515 520 525
His Lys Asp Asp Glu Glu Arg Phe Phe Pro Ser Ser Gly Val Leu Met
530 535 540
Phe Gly Lys Gln Gly Ala Gly Lys Asp Asn Val Asp Tyr Ser Ser Val
545 550 555 560
Met Leu Thr Ser Glu Glu Glu Ile Lys Thr Thr Asn Pro Val Ala Thr
565 570 575
Glu Gln Tyr Gly Val Val Ala Asp Asn Leu Gln Gln Gln Asn Ala Ala
580 585 590
Pro Ile Val Gly Ala Val Asn Ser Gln Gly Ala Leu Pro Gly Met Val
595 600 605
Trp Gln Asn Arg Asp Val Tyr Leu Gln Gly Pro Ile Trp Ala Lys Ile
610 615 620
Pro His Thr Asp Gly Asn Phe His Pro Ser Pro Leu Met Gly Gly Phe
625 630 635 640
Gly Leu Lys His Pro Pro Pro Gln Ile Leu Ile Lys Asn Thr Pro Val
645 650 655
Pro Ala Asp Pro Pro Thr Thr Phe Ser Gln Ala Lys Leu Ala Ser Phe
660 665 670
Ile Thr Gln Tyr Ser Thr Gly Gln Val Ser Val Glu Ile Glu Trp Glu
675 680 685
Leu Gln Lys Glu Asn Ser Lys Arg Trp Asn Pro Glu Ile Gln Tyr Thr
690 695 700
Ser Asn Tyr Tyr Lys Ser Thr Asn Val Asp Phe Ala Val Asn Thr Glu
705 710 715 720
Gly Thr Tyr Ser Glu Pro Arg Pro Ile Gly Thr Arg Tyr Leu Thr Arg
725 730 735
Asn Leu
<210>85
<211>738
<212>PRT
<213>vp1,克隆hu.42
<400>85
Met Ala Ala Asp Gly Tyr Leu Pro Asp Trp Leu Glu Asp Asn Leu Ser
1 5 10 15
Glu Gly Ile Arg Glu Trp Trp Asp Leu Lys Pro Gly Ala Pro Lys Pro
20 25 30
Lys Ala Asn Gln Gln Lys Gln Asp Asp Gly Arg Gly Leu Val Leu Pro
35 40 45
Gly Tyr Lys Tyr Leu Gly Pro Phe Asn Gly Leu Asp Lys Gly Glu Pro
50 55 60
Val Asn Ala Ala Asp Ala Ala Ala Leu Glu His Asp Lys Ala Tyr Asp
65 70 75 80
Gln Gln Leu Lys Ala Gly Asp Asn Pro Tyr Leu Arg Tyr Asn His Ala
85 90 95
Asp Ala Glu Phe Gln Glu Arg Leu Gln Glu Asp Thr Ser Phe Gly Gly
100 105 110
Asn Leu Gly Arg Ala Val Phe Gln Ala Lys Lys Arg Val Leu Glu Pro
115 120 125
Leu Gly Leu Val Glu Glu Ala Ala Lys Thr Ala Pro Gly Lys Lys Arg
130 135 140
Pro Val Glu Pro Ser Pro Gln Arg Ser Pro Asp Ser Ser Thr Gly Ile
145 150 155 160
Gly Lys Lys Gly Gln Gln Pro Ala Lys Lys Arg Leu Asn Phe Gly Gln
165 170 175
Thr Gly Asp Ser Glu Ser Val Pro Asp Pro Gln Pro Ile Gly Glu Pro
180 185 190
Pro Ala Gly Pro Ser Gly Leu Gly Ser Gly Thr Met Ala Ala Gly Gly
195 200 205
Gly Ala Pro Met Ala Asp Asn Asn Glu Gly Ala Asp Gly Val Gly Ser
210 215 220
Ser Ser Gly Asn Trp His Cys Asp Ser Thr Trp Leu Gly Asp Arg Val
225 230 235 240
Ile Thr Thr Ser Thr Arg Thr Trp Ala Leu Pro Thr Tyr Asn Asn His
245 250 255
Leu Tyr Lys Gln Ile Ser Asn Gly Thr Ser Gly Gly Ser Thr Asn Asp
260 265 270
Asn Thr Tyr Phe Gly Tyr Ser Thr Pro Trp Gly Tyr Phe Asp Phe Asn
275 280 285
Arg Phe His Cys His Phe Ser Pro Arg Asp Trp Gln Arg Leu Ile Asn
290 295 300
Asn Asn Trp Gly Phe Arg Pro Lys Arg Leu Ser Phe Lys Leu Phe Asn
305 310 315 320
Ile Gln Val Lys Glu Val Thr Gln Asn Glu Gly Thr Lys Thr Ile Ala
325 330 335
Asn Asn Leu Thr Ser Thr Ile Gln Val Phe Thr Asp Ser Glu Tyr Gln
340 345 350
Leu Pro Tyr Val Leu Gly Ser Ala His Gln Gly Cys Leu Pro Pro Phe
355 360 365
Pro Ala Asp Val Phe Met Ile Pro Gln Tyr Gly Tyr Leu Thr Leu Asn
370 375 380
Asn Gly Ser Gln Ala Val Gly Arg Ser Ser Phe Tyr Cys Leu Glu Tyr
385 390 395 400
Phe Pro Ser Gln Met Leu Arg Thr Gly Asn Asn Phe Glu Phe Ser Tyr
405 410 415
Thr Phe Glu Asp Val Pro Phe His Ser Ser Tyr Ala His Ser Gln Ser
420 425 430
Leu Asp Arg Leu Met Asn Pro Leu Ile Asp Gln Tyr Leu Tyr Tyr Leu
435 440 445
Ser Arg Thr Gln Ser Thr Gly Gly Thr Gln Gly Thr Gln Gln Leu Leu
450 455 460
Phe Ser Gln Ala Gly Pro Ala Asn Met Ser Ala Gln Ala Lys Asn Trp
465 470 475 480
Leu Pro Gly Pro Cys Tyr Arg Gln Gln Arg Val Ser Thr Thr Leu Ser
485 490 495
Gln Ser Asn Asn Ser Asn Phe Ala Trp Thr Gly Ala Thr Lys Tyr His
500 505 510
Leu Asn Gly Arg Asp Ser Leu Val Asn Pro Gly Val Ala Met Ala Thr
515 520 525
His Lys Asp Asp Glu Glu Arg Phe Phe Pro Ser Ser Gly Val Leu Met
530 535 540
Phe Gly Lys Gln Gly Ala Gly Arg Asp Asn Val Asp Tyr Ser Ser Val
545 550 555 560
Met Leu Thr Ser Glu Glu Glu Ile Lys Thr Thr Asn Pro Val Ala Thr
565 570 575
Glu Gln Tyr Gly Val Val Ala Asp Asn Leu Gln Gln Thr Asn Thr Gly
580 585 590
Pro Ile Val Gly Asn Val Asn Ser Gln Gly Ala Leu Pro Gly Met Val
595 600 605
Trp Gln Asn Arg Asp Val Tyr Leu Gln Gly Pro Ile Trp Ala Lys Ile
610 615 620
Pro His Thr Asp Gly Asn Phe His Pro Ser Pro Leu Met Gly Gly Leu
625 630 635 640
Gly Leu Lys His Pro Pro Pro Gln Ile Leu Ile Lys Asn Thr Pro Val
645 650 655
Pro Ala Asp Pro Pro Thr Thr Phe Ser Gln Ala Lys Leu Ala Ser Phe
660 665 670
Ile Thr Gln Tyr Ser Thr Gly Gln Val Ser Val Glu Ile Glu Trp Glu
675 680 685
Leu Gln Lys Glu Asn Ser Lys Arg Trp Asn Pro Glu Ile Gln Tyr Thr
690 695 700
Ser Asn Tyr Tyr Lys Ser Thr Asn Val Asp Phe Ala Val Asn Thr Glu
705 710 715 720
Gly Thr Tyr Ser Glu Pro Arg Pro Ile Gly Thr Arg Tyr Leu Thr Arg
725 730 735
Asn Leu
<210>86
<211>738
<212>PRT
<213>vp1,克隆rh.38
<400>86
Met Ala Ala Asp Gly Tyr Leu Pro Asp Trp Leu Glu Asp Asn Leu Ser
1 5 10 15
Glu Gly Ile Arg Glu Trp Trp Asp Leu Lys Pro Gly Ala Pro Lys Pro
20 25 30
Lys Ala Asn Gln Gln Lys Gln Asp Asp Gly Arg Gly Leu Val Leu Pro
35 40 45
Gly Tyr Lys Tyr Leu Gly Pro Phe Asn Gly Leu Asp Lys Gly Glu Pro
50 55 60
Val Asn Ala Ala Asp Ala Ala Ala Leu Glu His Asp Lys Ala Tyr Asp
65 70 75 80
Gln Gln Leu Lys Ala Gly Asp Asn Pro Tyr Leu Arg Tyr Asn His Ala
85 90 95
Asp Ala Glu Phe Gln Glu Arg Leu Gln Glu Asp Thr Ser Phe Gly Gly
100 105 110
Asn Leu Gly Arg Ala Val Phe Gln Ala Lys Lys Arg Val Leu Glu Pro
115 120 125
Leu Gly Leu Val Glu Glu Ala Ala Lys Thr Ala Pro Gly Lys Lys Arg
130 135 140
Pro Val Glu Pro Ser Pro Gln Arg Ser Pro Asp Ser Ser Thr Gly Ile
145 150 155 160
Gly Lys Lys Gly Gln Arg Pro Ala Lys Lys Arg Leu Asn Phe Gly Gln
165 170 175
Thr Gly Asp Ser Glu Ser Val Pro Asp Pro Gln Pro Ile Gly Glu Pro
180 185 190
Pro Ala Gly Pro Ser Gly Leu Gly Ser Gly Thr Met Ala Ala Gly Gly
195 200 205
Gly Ala Pro Met Ala Asp Asn Asn Glu Gly Ala Asp Gly Val Gly Ser
210 215 220
Ser Ser Gly Asn Trp His Cys Asp Ser Thr Trp Leu Gly Asp Arg Val
225 230 235 240
Ile Thr Thr Ser Thr Arg Thr Trp Ala Leu Pro Thr Tyr Asn Asn His
245 250 255
Leu Tyr Lys Gln Ile Ser Asn Gly Thr Ser Gly Gly Ser Thr Asn Asp
260 265 270
Asn Thr Tyr Phe Gly Tyr Ser Thr Pro Trp Gly Tyr Phe Asp Phe Asn
275 280 285
Arg Phe His Cys His Phe Ser Pro Arg Asp Trp Gln Arg Leu Ile Asn
290 295 300
Asn Asn Trp Gly Phe Arg Pro Lys Arg Leu Ser Phe Lys Pro Phe Asn
305 310 315 320
Ile Gln Val Lys Glu Val Thr Gln Asn Glu Gly Thr Lys Thr Ile Ala
325 330 335
Asn Asn Leu Thr Ser Thr Ile Gln Val Phe Thr Asp Ser Glu Tyr Gln
340 345 350
Leu Pro Tyr Val Leu Gly Ser Ala His Gln Gly Cys Leu Pro Pro Phe
355 360 365
Pro Ala Asp Val Phe Met Ile Pro Gln Tyr Gly Tyr Leu Thr Leu Asn
370 375 380
Asn Gly Ser Gln Ala Val Gly Arg Ser Ser Phe Tyr Cys Leu Glu Tyr
385 390 395 400
Phe Pro Ser Gln Met Leu Arg Thr Gly Asn Asn Phe Glu Phe Ser Tyr
405 410 415
Thr Phe Glu Asp Val Pro Phe His Ser Ser Tyr Ala His Ser Gln Ser
420 425 430
Leu Asp Arg Leu Met Asn Pro Leu Ile Asp Gln Tyr Leu His Tyr Leu
435 440 445
Ser Arg Thr Gln Ser Thr Gly Gly Thr Gln Gly Thr Gln Gln Leu Leu
450 455 460
Phe Ser Gln Ala Gly Pro Ala Asn Met Ser Ala Gln Ala Lys Asn Trp
465 470 475 480
Leu Pro Gly Pro Cys Tyr Arg Gln Gln Arg Val Ser Thr Thr Leu Ser
485 490 495
Gln Asn Asn Asn Ser Asn Phe Ala Trp Thr Gly Ala Thr Lys Tyr His
500 505 510
Leu Asn Gly Arg Asp Ser Leu Val Asn Pro Gly Val Ala Met Ala Thr
515 520 525
His Lys Asp Asp Glu Glu Arg Phe Phe Pro Ser Ser Gly Val Leu Met
530 535 540
Phe Gly Lys Gln Gly Ala Gly Arg Asp Asn Val Asp Tyr Ser Ser Val
545 550 555 560
Met Leu Thr Ser Glu Glu Glu Ile Lys Thr Thr Asn Pro Val Ala Thr
565 570 575
Glu Gln Tyr Gly Val Val Ala Asp Asn Leu Gln Gln Thr Asn Thr Gly
580 585 590
Pro Ile Val Gly Asn Val Asn Ser Gln Gly Ala Leu Pro Gly Met Val
595 600 605
Trp Gln Asn Arg Asp Val Tyr Leu Gln Gly Pro Ile Trp Ala Lys Ile
610 615 620
Pro His Thr Asp Gly Asn Phe His Pro Ser Pro Leu Met Gly Gly Phe
625 630 635 640
Gly Leu Lys His Pro Pro Pro Gln Ile Leu Ile Lys Asn Thr Pro Val
645 650 655
Pro Ala Asp Pro Pro Thr Thr Phe Ser Gln Ala Lys Leu Ala Ser Phe
660 665 670
Ile Thr Gln Tyr Ser Thr Gly Gln Val Ser Val Glu Ile Glu Trp Glu
675 680 685
Leu Gln Lys Glu Asn Ser Lys Arg Trp Asn Pro Glu Ile Gln Tyr Thr
690 695 700
Ser Asn Tyr Tyr Lys Ser Thr Asn Val Asp Phe Ala Val Asn Thr Glu
705 710 715 720
Gly Thr Tyr Ser Glu Pro Arg Pro Ile Gly Thr Arg Tyr Leu Thr Arg
725 730 735
Asn Leu
<210>87
<211>738
<212>PRT
<213>vp1,克隆hu.40
<400>87
Met Ala Ala Asp Gly Tyr Leu Pro Asp Trp Leu Glu Asp Asn Leu Ser
1 5 10 15
Glu Gly Ile Arg Glu Trp Trp Asp Leu Lys Pro Gly Ala Pro Lys Pro
20 25 30
Lys Ala Asn Gln Gln Lys Gln Asp Asp Gly Arg Gly Leu Val Leu Pro
35 40 45
Gly Tyr Lys Tyr Leu Gly Pro Phe Asn Gly Leu Asp Lys Gly Glu Pro
50 55 60
Val Asn Ala Ala Asp Ala Ala Ala Leu Glu His Asp Lys Ala Tyr Asp
65 70 75 80
Gln Gln Leu Lys Ala Gly Asp Asn Pro Tyr Leu Arg Tyr Asn His Ala
85 90 95
Asp Ala Glu Phe Gln Glu Arg Leu Gln Glu Asp Thr Ser Phe Gly Gly
100 105 110
Asn Leu Gly Arg Ala Val Phe Gln Ala Lys Lys Arg Val Leu Glu Pro
115 120 125
Leu Gly Leu Val Glu Glu Ala Ala Lys Thr Ala Pro Gly Lys Lys Arg
130 135 140
Pro Val Glu Pro Ser Pro Gln Arg Ser Pro Asp Ser Ser Thr Gly Ile
145 150 155 160
Gly Lys Lys Gly Gln Gln Pro Ala Lys Lys Arg Leu Ser Phe Gly Gln
165 170 175
Thr Gly Asp Ser Glu Ser Val Pro Asp Pro Gln Pro Ile Gly Glu Pro
180 185 190
Pro Ala Gly Pro Ser Gly Leu Gly Ser Gly Thr Met Ala Ala Gly Gly
195 200 205
Gly Ala Pro Met Ala Asp Asn Asn Glu Gly Ala Asp Gly Val Gly Ser
210 215 220
Ser Ser Gly Asn Trp His Cys Asp Ser Thr Trp Leu Gly Asp Arg Val
225 230 235 240
Ile Thr Thr Ser Thr Arg Thr Trp Ala Leu Pro Thr Tyr Asn Asn His
245 250 255
Leu Tyr Lys Gln Ile Ser Asn Gly Thr Ser Gly Gly Ser Thr Asn Asp
260 265 270
Asn Thr Tyr Phe Gly Tyr Ser Thr Pro Trp Gly Tyr Phe Asp Phe Asn
275 280 285
Arg Phe His Cys His Phe Ser Pro Arg Asp Trp Gln Arg Leu Ile Asn
290 295 300
Asn Asn Trp Gly Phe Arg Pro Lys Arg Leu Ser Phe Lys Leu Phe Asn
305 310 315 320
Ile Gln Val Lys Glu Val Thr Gln Asn Glu Gly Thr Lys Thr Ile Ala
325 330 335
Asn Asn Leu Thr Ser Thr Ile Gln Val Phe Thr Asp Ser Glu Tyr Gln
340 345 350
Leu Pro Tyr Val Leu Gly Ser Ala His Gln Gly Cys Leu Pro Pro Phe
355 360 365
Pro Ala Asp Val Phe Met Ile Pro Gln Tyr Gly Tyr Leu Thr Leu Asn
370 375 380
Asn Gly Ser Gln Ala Val Gly Arg Ser Ser Phe Tyr Cys Leu Glu Tyr
385 390 395 400
Phe Pro Ser Gln Met Leu Arg Thr Gly Asn Asn Ser Glu Phe Ser Tyr
405 410 415
Thr Phe Glu Asp Val Pro Phe His Ser Ser Tyr Ala His Ser Gln Ser
420 425 430
Leu Asp Arg Leu Met Asn Pro Leu Ile Asp Gln Tyr Leu Tyr Tyr Leu
435 440 445
Ser Arg Thr Gln Ser Thr Gly Gly Thr Gln Gly Thr Gln Gln Leu Leu
450 455 460
Phe Ser Gln Ala Gly Pro Ala Asn Met Ser Ala Gln Ala Lys Asn Trp
465 470 475 480
Leu Pro Gly Pro Cys Tyr Arg Gln Gln Arg Val Ser Thr Thr Leu Ser
485 490 495
Gln Asn Asn Asn Ser Asn Phe Ala Trp Thr Gly Ala Thr Lys Tyr His
500 505 510
Leu Asn Gly Arg Asp Ser Leu Val Asn Pro Gly Val Ala Met Ala Thr
515 520 525
His Lys Asp Asp Glu Glu Arg Phe Phe Pro Ser Ser Gly Val Leu Met
530 535 540
Phe Gly Lys Gln Gly Ala Gly Arg Asp Asn Val Asp Tyr Ser Ser Val
545 550 555 560
Met Leu Thr Ser Glu Glu Glu Ile Lys Thr Thr Asn Pro Val Ala Thr
565 570 575
Glu Gln Tyr Gly Val Val Ala Asp Asn Leu Gln Gln Thr Asn Thr Gly
580 585 590
Pro Ile Val Gly Asn Val Asn Ser Gln Gly Ala Leu Pro Gly Met Val
595 600 605
Trp Gln Asn Arg Asp Val Tyr Leu Gln Gly Pro Ile Trp Ala Lys Ile
610 615 620
Pro His Thr Asp Gly Asn Phe His Pro Ser Pro Leu Met Gly Gly Phe
625 630 635 640
Gly Leu Lys His Pro Pro Pro Gln Ile Leu Ile Lys Asn Thr Pro Val
645 650 655
Pro Ala Asp Pro Pro Thr Thr Phe Ser Gln Ala Lys Leu Ala Ser Phe
660 665 670
Ile Thr Gln Tyr Ser Thr Gly Gln Val Ser Val Glu Ile Glu Trp Glu
675 680 685
Leu Gln Lys Glu Asn Ser Lys Arg Trp Asn Pro Glu Ile Gln Tyr Thr
690 695 700
Ser Asn Tyr Tyr Lys Ser Thr Asn Val Asp Phe Ala Val Asn Thr Glu
705 710 715 720
Gly Thr Tyr Ser Glu Pro Arg Pro Ile Gly Thr Arg Tyr Leu Thr Arg
725 730 735
Asn Leu
<210>88
<211>738
<212>PRT
<213>vp1,克隆hu.37
<400>88
Met Ala Ala Asp Gly Tyr Leu Pro Asp Trp Leu Glu Asp Asn Leu Ser
1 5 10 15
Glu Gly Ile Arg Glu Trp Trp Asp Leu Lys Pro Gly Ala Pro Lys Pro
20 25 30
Lys Ala Asn Gln Gln Lys Gln Asp Asp Gly Arg Gly Leu Val Leu Pro
35 40 45
Gly Tyr Lys Tyr Leu Gly Pro Phe Asn Gly Leu Asp Lys Gly Glu Pro
50 55 60
Val Asn Ala Ala Asp Ala Ala Ala Leu Glu His Asp Lys Ala Tyr Asp
65 70 75 80
Gln Gln Leu Lys Ala Gly Asp Asn Pro Tyr Leu Arg Tyr Asn His Ala
85 90 95
Asp Ala Glu Phe Gln Glu Arg Leu Gln Glu Asp Thr Ser Phe Gly Gly
100 105 110
Asn Leu Gly Arg Ala Val Phe Gln Ala Lys Lys Arg Val Leu Glu Pro
115 120 125
Leu Gly Leu Val Glu Glu Ala Ala Lys Thr Ala Pro Gly Lys Lys Arg
130 135 140
Pro Val Glu Pro Ser Pro Gln Arg Ser Pro Asp Ser Ser Thr Gly Ile
145 150 155 160
Gly Lys Lys Gly Gln Gln Pro Ala Lys Lys Arg Leu Asn Phe Gly Gln
165 170 175
Thr Gly Asp Ser Glu Ser Val Pro Asp Pro Gln Pro Ile Gly Glu Pro
180 185 190
Pro Ala Gly Pro Ser Gly Leu Gly Ser Gly Thr Met Ala Ala Gly Gly
195 200 205
Gly Ala Pro Met Ala Asp Asn Asn Glu Gly Ala Asp Gly Val Gly Ser
210 215 220
Ser Ser Gly Asn Trp His Cys Asp Ser Thr Trp Leu Gly Asp Arg Val
225 230 235 240
Ile Thr Thr Ser Thr Arg Thr Trp Ala Leu Pro Thr Tyr Asn Asn His
245 250 255
Leu Tyr Lys Gln Ile Ser Asn Gly Thr Ser Gly Gly Ser Thr Asn Asp
260 265 270
Asn Thr Tyr Phe Gly Tyr Ser Thr Pro Trp Gly Tyr Phe Asp Phe Asn
275 280 285
Arg Phe His Cys His Phe Ser Pro Arg Asp Trp Gln Arg Leu Ile Asn
290 295 300
Asn Asn Trp Gly Phe Arg Pro Lys Arg Leu Ser Phe Lys Leu Phe Asn
305 310 315 320
Ile Gln Val Lys Glu Val Thr Gln Asn Glu Gly Thr Lys Thr Ile Ala
325 330 335
Asn Asn Leu Thr Ser Thr Ile Gln Val Phe Thr Asp Ser Glu Tyr Gln
340 345 350
Leu Pro Tyr Val Leu Gly Ser Ala His Gln Gly Cys Leu Pro Pro Phe
355 360 365
Pro Ala Asp Val Phe Met Ile Pro Gln Tyr Gly Tyr Leu Thr Leu Asn
370 375 380
Asn Gly Ser Gln Ala Val Gly Arg Ser Ser Phe Tyr Cys Leu Glu Tyr
385 390 395 400
Phe Pro Ser Gln Met Leu Arg Thr Gly Asn Asn Phe Glu Phe Ser Tyr
405 410 415
Thr Phe Glu Asp Val Pro Phe His Ser Ser Tyr Ala His Ser Gln Ser
420 425 430
Leu Asp Arg Leu Met Asn Pro Leu Ile Asp Gln Tyr Leu Tyr Tyr Leu
435 440 445
Ser Arg Thr Gln Ser Thr Gly Gly Thr Gln Gly Thr Gln Gln Leu Leu
450 455 460
Phe Ser Gln Ala Gly Pro Ala Asn Met Ser Ala Gln Ala Lys Asn Trp
465 470 475 480
Leu Pro Gly Pro Cys Tyr Arg Gln Gln Arg Val Ser Thr Thr Leu Ser
485 490 495
Gln Asn Asn Asn Ser Asn Phe Ala Trp Thr Gly Ala Thr Lys Tyr His
500 505 510
Leu Asn Gly Arg Asp Ser Leu Val Asn Pro Gly Val Ala Met Ala Thr
515 520 525
His Lys Asp Asp Glu Glu Arg Phe Phe Pro Ser Ser Gly Val Leu Met
530 535 540
Phe Gly Lys Gln Gly Ala Gly Arg Asp Asn Val Asp Tyr Ser Ser Val
545 550 555 560
Met Leu Thr Ser Glu Glu Glu Ile Lys Thr Thr Asn Pro Val Ala Thr
565 570 575
Glu Gln Tyr Gly Val Val Ala Asp Asn Leu Gln Gln Thr Asn Thr Gly
580 585 590
Pro Ile Val Gly Asn Val Asn Ser Gln Gly Ala Leu Pro Gly Met Val
595 600 605
Trp Gln Asn Arg Asp Val Tyr Leu Gln Gly Pro Ile Trp Ala Lys Ile
610 615 620
Pro His Thr Asp Gly Asn Phe His Pro Ser Pro Leu Met Gly Gly Phe
625 630 635 640
Gly Leu Lys His Pro Pro Pro Gln Ile Leu Ile Lys Asn Thr Pro Val
645 650 655
Pro Ala Asp Pro Pro Thr Thr Phe Ser Gln Ala Lys Leu Ala Ser Phe
660 665 670
Ile Thr Gln Tyr Ser Thr Gly Gln Val Ser Val Glu Ile Glu Trp Glu
675 680 685
Leu Gln Lys Glu Asn Ser Lys Arg Trp Asn Pro Glu Ile Gln Tyr Thr
690 695 700
Ser Asn Tyr Tyr Lys Ser Thr Asn Val Asp Phe Ala Val Asn Thr Glu
705 710 715 720
Gly Thr Tyr Ser Glu Pro Arg Pro Ile Gly Thr Arg Tyr Leu Thr Arg
725 730 735
Asn Leu
<210>89
<21l>738
<212>PRT
<213>vpl,克隆rh.39
<400>89
Met Ala Ala Asp Gly Tyr Leu Pro Asp Trp Leu Glu Asp Asn Leu Ser
1 5 10 15
Glu Gly Ile Arg Glu Trp Trp Asp Leu Lys Pro Gly Ala Pro Lys Pro
20 25 30
Lys Ala Asn Gln Gln Lys Gln Asp Asp Gly Arg Gly Leu Val Leu Pro
35 40 45
Gly Tyr Lys Tyr Leu Gly Pro Phe Asn Gly Leu Asp Lys Gly Glu Pro
50 55 60
Val Asn Ala Ala Asp Ala Ala Ala Leu Glu His Asp Lys Ala Tyr Asp
65 70 75 80
Gln Gln Leu Lys Ala Gly Asp Asn Pro Tyr Leu Arg Tyr Asn His Ala
85 90 95
Asp Ala Glu Phe Gln Glu Arg Leu Gln Glu Asp Thr Ser Phe Gly Gly
100 105 110
Asn Leu Gly Arg Ala Val Phe Gln Ala Lys Lys Arg Val Leu Glu Pro
115 120 125
Leu Gly Leu Val Glu Glu Ala Ala Lys Thr Ala Pro Gly Lys Lys Arg
130 135 140
Pro Val Glu Pro Ser Pro Gln Arg Ser Pro Asp Ser Ser Thr Gly Ile
145 150 155 160
Gly Lys Lys Gly Gln Gln Pro Ala Lys Lys Arg Leu Asn Phe Gly Gln
165 170 175
Thr Gly Asp Ser Glu Ser Val Pro Asp Pro Gln Pro Ile Gly Glu Pro
180 185 190
Pro Ala Gly Pro Ser Gly Leu Gly Ser Gly Thr Met Ala Ala Gly Gly
195 200 205
Gly Ala Pro Met Ala Asp Asn Asn Glu Gly Ala Asp Gly Val Gly Ser
210 215 220
Ser Ser Gly Asn Trp His Cys Asp Ser Thr Trp Leu Gly Asp Arg Val
225 230 235 240
Ile Thr Thr Ser Thr Arg Thr Trp Ala Leu Pro Thr Tyr Asn Asn His
245 250 255
Leu Tyr Lys Gln Ile Ser Asn Gly Thr Ser Gly Gly Ser Thr Asn Asp
260 265 270
Asn Thr Tyr Phe Gly Tyr Ser Thr Pro Trp Gly Tyr Phe Asp Phe Asn
275 280 285
Arg Phe His Cys His Phe Ser Pro Arg Asp Trp Gln Arg Leu Ile Asn
290 295 300
Asn Asn Trp Gly Phe Arg Pro Lys Arg Leu Ser Phe Lys Leu Phe Asn
305 310 315 320
Ile Gln Val Lys Glu Val Thr Gln Asn Glu Gly Thr Lys Thr Ile Ala
325 330 335
Asn Asn Leu Thr Ser Thr Ile Gln Val Phe Thr Asp Ser Glu Tyr Gln
340 345 350
Leu Pro Tyr Val Leu Gly Ser Ala His Gln Gly Cys Leu Pro Pro Phe
355 360 365
Pro Ala Asp Val Phe Met Ile Pro Gln Tyr Gly Tyr Leu Thr Leu Asn
370 375 380
Asn Gly Ser Gln Ala Val Gly Arg Ser Ser Phe Tyr Cys Leu Glu Tyr
385 390 395 400
Phe Pro Ser Gln Met Leu Arg Thr Gly Asn Asn Phe Glu Phe Ser Tyr
405 410 415
Thr Phe Glu Asp Val Pro Phe His Ser Ser Tyr Ala His Ser Gln Ser
420 425 430
Leu Asp Arg Leu Met Asn Pro Leu Ile Asp Gln Tyr Leu Tyr Tyr Leu
435 440 445
Ser Arg Thr Gln Ser Thr Gly Gly Thr Gln Gly Thr Gln Gln Leu Leu
450 455 460
Phe Ser Gln Ala Gly Pro Ala Asn Met Ser Ala Gln Ala Lys Asn Trp
465 470 475 480
Leu Pro Gly Pro Cys Tyr Arg Gln Gln Arg Val Ser Thr Thr Leu Ser
485 490 495
Gln Asn Asn Asn Ser Asn Phe Ala Trp Thr Gly Ala Thr Lys Tyr His
500 505 510
Leu Asn Gly Arg Asp Ser Leu Val Asn Pro Gly Val Ala Met Ala Thr
515 520 525
His Lys Asp Asp Glu Glu Arg Phe Phe Pro Ser Ser Gly Val Leu Met
530 535 540
Phe Gly Lys Gln Gly Ala Gly Arg Asp Asn Val Asp Tyr Ser Ser Val
545 550 555 560
Met Leu Thr Ser Glu Glu Glu Ile Lys Thr Thr Asn Pro Val Ala Thr
565 570 575
Glu Gln Tyr Gly Val Val Ala Asp Asn Leu Gln Gln Thr Asn Thr Gly
580 585 590
Pro Ile Val Gly Asn Val Asn Ser Gln Gly Ala Leu Pro Gly Met Val
595 600 605
Trp Gln Asn Arg Asp Val Tyr Leu Gln Gly Pro Ile Trp Ala Lys Ile
610 615 620
Pro His Thr Asp Gly Asn Phe His Pro Ser Pro Leu Met Gly Gly Phe
625 630 635 640
Gly Leu Lys His Pro Pro Pro Gln Ile Leu Ile Lys Asn Thr Pro Val
645 650 655
Pro Ala Asp Pro Pro Thr Thr Phe Ser Gln Ala Lys Leu Ala Ser Phe
660 665 670
Ile Thr Gln Tyr Ser Thr Gly Gln Val Ser Val Glu Ile Glu Trp Glu
675 680 685
Leu Gln Lys Glu Asn Ser Lys Arg Trp Asn Pro Glu Ile Gln Tyr Thr
690 695 700
Ser Asn Tyr Tyr Lys Ser Thr Asn Val Asp Phe Ala Val Asn Thr Glu
705 710 715 720
Gly Thr Tyr Ser Glu Pro Arg Pro Ile Gly Thr Arg Tyr Leu Thr Arg
725 730 735
Asn Leu
<210>90
<211>738
<212>PRT
<213>vp1,克隆AAV4407
<400>90
Met Ala Ala Asp Gly Tyr Leu Pro Asp Trp Leu Glu Asp Asn Leu Ser
l 5 10 15
Glu Gly Ile Arg Glu Trp Trp Asp Leu Lys Pro Gly Ala Pro Lys Pro
20 25 30
Lys Ala Asn Gln Gln Lys Gln Asp Asp Gly Arg Gly Leu Val Leu Pro
35 40 45
Gly Tyr Lys Tyr Leu Gly Pro Phe Asn Gly Leu Asp Lys Gly Glu Pro
50 55 60
Val Asn Ala Ala Asp Ala Ala Ala Leu Glu His Asp Lys Ala Tyr Asp
65 70 75 80
Gln Gln Leu Lys Ala Gly Asp Asn Pro Tyr Leu Arg Tyr Asn His Ala
85 90 95
Asp Ala Glu Phe Gln Glu Arg Leu Gln Glu Asp Thr Ser Phe Gly Gly
100 105 110
Asn Leu Gly Arg Ala Val Phe Gln Ala Lys Lys Arg Val Leu Glu Pro
115 120 125
Leu Gly Leu Val Glu Glu Ala Ala Lys Thr Ala Pro Gly Lys Lys Arg
130 135 140
Pro Val Glu Pro Ser Pro Gln Arg Ser Pro Asp Ser Ser Thr Gly Ile
145 150 155 160
Gly Lys Lys Gly Gln Gln Pro Ala Lys Lys Arg Leu Asn Phe Gly Gln
165 170 175
Thr Gly Asp Ser Glu Ser Val Pro Asp Pro Gln Pro Ile Gly Glu Pro
180 185 190
Pro Ala Gly Pro Ser Gly Leu Gly Ser Gly Thr Met Ala Ala Gly Gly
195 200 205
Gly Ala Pro Met Ala Asp Asn Asn Glu Gly Ala Asp Gly Val Gly Ser
210 215 220
Ser Ser Gly Asn Trp His Cys Asp Ser Thr Trp Leu Gly Asp Arg Val
225 230 235 240
Ile Thr Thr Ser Thr Arg Thr Trp Ala Leu Pro Thr Tyr Asn Asn His
245 250 255
Leu Tyr Lys Gln Ile Ser Asn Gly Thr Ser Gly Gly Ser Thr Asn Asp
260 265 270
Asn Thr Tyr Phe Gly Tyr Ser Thr Pro Trp Gly Tyr Phe Asp Phe Asn
275 280 285
Arg Phe His Cys His Phe Ser Pro Arg Asp Trp Gln Arg Leu Ile Asn
290 295 300
Asn Asn Trp Gly Phe Arg Pro Lys Arg Leu Ser Phe Lys Leu Phe Asn
305 310 315 320
Ile Gln Val Lys Glu Val Thr Gln Asn Glu Gly Thr Lys Thr Ile Ala
325 330 335
Asn Asn Leu Thr Ser Thr Ile Gln Val Phe Thr Asp Ser Glu Tyr Gln
340 345 350
Leu Pro Tyr Val Leu Gly Ser Ala His Gln Gly Cys Leu Pro Pro Phe
355 360 365
Pro Ala Asp Val Phe Met Ile Pro Gln Tyr Gly Tyr Leu Thr Leu Asn
370 375 380
Asn Gly Ser Gln Ala Val Gly Arg Ser Ser Phe Tyr Cys Leu Glu Tyr
385 390 395 400
Phe Pro Ser Gln Met Leu Arg Thr Gly Asn Asn Phe Glu Phe Ser Tyr
405 410 415
Thr Phe Glu Asp Val Pro Phe His Ser Ser Tyr Ala His Ser Gln Ser
420 425 430
Leu Asp Arg Leu Met Asn Pro Leu Ile Asp Gln Tyr Leu Tyr Tyr Leu
435 440 445
Ser Arg Thr Gln Ser Thr Gly Gly Thr Gln Gly Thr Gln Gln Leu Leu
450 455 460
Phe Ser Gln Ala Gly Pro Ala Asn Met Ser Ala Gln Ala Lys Asn Trp
465 470 475 480
Leu Pro Gly Pro Cys Tyr Arg Gln Gln Arg Val Ser Thr Thr Leu Ser
485 490 495
Gln Asn Asn Asn Ser Asn Phe Ala Trp Thr Gly Ala Thr Lys Tyr His
500 505 510
Leu Asn Gly Arg Asp Ser Leu Val Asn Pro Gly Val Ala Met Ala Thr
515 520 525
His Lys Asp Asp Glu Glu Arg Phe Phe Pro Ser Ser Gly Val Leu Met
530 535 540
Phe Gly Lys Gln Gly Ala Gly Arg Asp Asn Val Asp Tyr Ser Ser Val
545 550 555 560
Met Leu Thr Ser Glu Glu Glu Ile Lys Thr Thr Asn Pro Val Ala Thr
565 570 575
Glu Gln Tyr Gly Val Val Ala Asp Asn Leu Gln Gln Thr Asn Thr Gly
580 585 590
Pro Ile Val Gly Asn Val Asn Ser Gln Gly Ala Leu Pro Gly Met Val
595 600 605
Trp Gln Asn Arg Asp Val Tyr Leu Gln Gly Pro Ile Trp Ala Lys Ile
610 615 620
Pro His Thr Asp Gly Asn Phe His Pro Ser Pro Leu Met Gly Gly Phe
625 630 635 640
Gly Leu Lys His Pro Pro Pro Gln Ile Leu Ile Lys Asn Thr Pro Val
645 650 655
Pro Ala Asp Pro Pro Thr Thr Phe Ser Gln Ala Lys Leu Ala Ser Phe
660 665 670
Ile Thr Gln Tyr Ser Thr Gly Gln Val Ser Val Glu Ile Glu Trp Glu
675 680 685
Leu Gln Lys Glu Asn Ser Lys Arg Trp Asn Pro Glu Ile Gln Tyr Thr
690 695 700
Ser Asn Tyr Tyr Lys Ser Thr Asn Val Asp Phe Ala Val Asn Thr Glu
705 710 715 720
Gly Thr Tyr Ser Glu Pro Arg Pro Ile Gly Thr Arg Tyr Leu Thr Arg
725 730 735
Asn Leu
<210>91
<211>738
<212>PRT
<213>vp1,克隆hu.41
<400>91
Met Ala Ala Asp Gly Tyr Leu Pro Asp Trp Leu Glu Asp Asn Leu Ser
1 5 10 15
Glu Gly Ile Arg Glu Trp Trp Asp Leu Lys Pro Gly Ala Pro Lys Pro
20 25 30
Lys Ala Asn Gln Gln Lys Gln Asp Asp Gly Arg Gly Leu Val Leu Pro
35 40 45
Gly Tyr Lys Tyr Leu Gly Pro Phe Asn Gly Leu Asp Lys Gly Glu Pro
50 55 60
Val Asn Ala Ala Asp Ala Ala Ala Leu Glu His Asp Lys Ala Tyr Asp
65 70 75 80
Gln Gln Leu Lys Ala Gly Asp Asn Pro Tyr Leu Arg Tyr Asn His Ala
85 90 95
Asp Ala Glu Phe Gln Glu Arg Leu Gln Glu Asp Thr Ser Phe Gly Gly
100 105 110
Asn Leu Gly Arg Ala Val Phe Gln Ala Lys Lys Arg Val Leu Glu Pro
115 120 125
Leu Gly Pro Val Glu Glu Ala Ala Lys Thr Ala Pro Gly Lys Lys Arg
130 135 140
Pro Val Glu Pro Pro Pro Gln Arg Ser Pro Asp Ser Ser Thr Gly Ile
145 150 155 160
Gly Lys Lys Gly Gln Gln Pro Ala Lys Lys Arg Leu Asn Phe Gly Gln
165 170 175
Thr Gly Asp Ser Glu Ser Val Pro Asp Pro Gln Pro Ile Gly Glu Pro
180 185 190
Pro Ala Gly Pro Ser Gly Leu Gly Ser Gly Thr Met Ala Ala Gly Gly
195 200 205
Gly Ala Pro Met Ala Asp Asn Asn Glu Gly Ala Asp Gly Val Gly Ser
210 215 220
Ser Ser Gly Asn Trp His Cys Asp Ser Thr Trp Leu Gly Asp Arg Val
225 230 235 240
Ile Thr Thr Ser Thr Arg Thr Trp Ala Leu Pro Thr Tyr Asn Asn His
245 250 255
Leu Tyr Lys Gln Ile Ser Asn Gly Thr Ser Gly Gly Ser Thr Asn Asp
260 265 270
Asn Thr Tyr Phe Gly Tyr Ser Thr Pro Trp Gly Tyr Phe Asp Phe Asn
275 280 285
Arg Phe His Cys His Phe Ser Pro Arg Asp Trp Gln Arg Leu Ile Asn
290 295 300
Asn Asn Trp Gly Phe Arg Pro Lys Arg Leu Ser Phe Lys Leu Phe Asn
305 310 315 320
Ile Gln Val Lys Glu Val Thr Gln Asn Glu Gly Thr Lys Thr Val Ala
325 330 335
Asn Asn Leu Thr Ser Thr Ile Gln Val Phe Thr Asp Ser Glu Tyr Gln
340 345 350
Leu Pro Tyr Val Leu Gly Ser Ala His Gln Gly Cys Leu Pro Pro Phe
355 360 365
Pro Ala Asp Val Phe Met Ile Pro Gln Tyr Gly Tyr Leu Thr Leu Asn
370 375 380
Asn Gly Ser Gln Ala Val Gly Arg Ser Ser Phe Tyr Cys Leu Glu Tyr
385 390 395 400
Phe Pro Ser Gln Met Leu Arg Thr Gly Asn Asn Phe Glu Phe Ser Tyr
405 410 415
Thr Phe Glu Asp Val Pro Phe His Ser Ser Tyr Ala His Ser Gln Ser
420 425 430
Leu Asp Arg Leu Met Asn Pro Leu Ile Asp Gln Tyr Leu Tyr Tyr Leu
435 440 445
Ser Arg Thr Gln Ser Thr Gly Gly Thr Gln Gly Thr Gln Gln Leu Leu
450 455 460
Phe Ser Gln Ala Gly Pro Ala Asn Met Ser Ala Gln Ala Lys Asn Trp
465 470 475 480
Leu Pro Gly Pro Cys Tyr Arg Gln Gln Arg Val Ser Thr Thr Leu Ser
485 490 495
Gln Asn Asn Asn Ser Asn Phe Ala Trp Thr Gly Ala Thr Lys Tyr His
500 505 510
Leu Asn Gly Arg Asp Ser Leu Val Asn Pro Gly Val Ala Met Ala Thr
515 520 525
His Lys Asp Asp Glu Glu Arg Phe Phe Pro Ser Ser Gly Val Leu Met
530 535 540
Phe Gly Lys Gln Gly Ala Gly Arg Asp Asn Val Asp Tyr Ser Ser Val
545 550 555 560
Met Leu Thr Ser Glu Glu Glu Ile Lys Thr Thr Asn Pro Val Ala Thr
565 570 575
Glu Gln Tyr Gly Val Val Ala Asp Asn Leu Gln Gln Thr Asn Thr Gly
580 585 590
Pro Ile Val Gly Asn Val Asn Ser Gln Gly Ala Leu Pro Gly Met Val
595 600 605
Trp Gln Asn Arg Asp Val Tyr Leu Gln Gly Pro Ile Trp Ala Lys Ile
610 615 620
Pro His Thr Asp Gly Asn Phe His Pro Ser Pro Leu Met Gly Gly Phe
625 630 635 640
Gly Leu Lys His Pro Pro Pro Gln Ile Leu Ile Lys Asn Thr Pro Val
645 650 655
Pro Ala Asp Pro Pro Thr Thr Phe Ser Gln Ala Lys Leu Ala Ser Phe
660 665 670
Ile Thr Gln Tyr Ser Thr Gly Gln Val Ser Val Glu Ile Glu Trp Glu
675 680 685
Leu Gln Lys Glu Asn Ser Lys Arg Trp Asn Pro Glu Ile Gln Tyr Thr
690 695 700
Ser Asn Tyr Tyr Lys Ser Thr Asn Val Asp Phe Ala Val Asn Thr Glu
705 710 715 720
Gly Thr Tyr Ser Glu Pro Arg Pro Ile Gly Thr Arg Tyr Leu Thr Arg
725 730 735
Asn Leu
<210>92
<211>738
<212>PRT
<213>vp1,克隆rh.40
<400>92
Met Ala Ala Asp Gly Tyr Leu Pro Asp Trp Leu Glu Asp Asn Leu Ser
1 5 10 15
Glu Gly Ile Arg Glu Trp Trp Asp Leu Lys Pro Gly Ala Pro Lys Pro
20 25 30
Lys Ala Asn Gln Gln Lys Gln Asp Asp Gly Arg Gly Leu Val Leu Pro
35 40 45
Gly Tyr Lys Tyr Leu Gly Pro Phe Asn Gly Leu Asp Lys Gly Glu Pro
50 55 60
Val Asn Ala Ala Asp Ala Ala Ala Leu Glu His Asp Lys Ala Tyr Asp
65 70 75 80
Gln Gln Leu Lys Ala Gly Asp Asn Pro Tyr Leu Arg Tyr Asn His Ala
85 90 95
Asp Ala Glu Phe Gln Glu Arg Leu Gln Glu Asp Thr Ser Phe Gly Gly
100 105 110
Asn Leu Gly Arg Ala Val Phe Gln Ala Lys Lys Arg Val Leu Glu Pro
115 120 125
Leu Gly Leu Val Glu Glu Ala Ala Lys Thr Ala Pro Gly Lys Lys Arg
130 135 140
Pro Val Glu Pro Ser Pro Gln Arg Ser Pro Asp Ser Ser Thr Gly Ile
145 150 155 160
Gly Lys Lys Gly Gln Gln Pro Ala Lys Lys Arg Leu Asn Phe Gly Gln
165 170 175
Thr Gly Asp Ser Glu Ser Val Pro Asp Pro Gln Pro Ile Gly Glu Pro
180 185 190
Pro Ala Gly Pro Ser Gly Leu Gly Ser Gly Thr Met Ala Ala Gly Gly
195 200 205
Gly Ala Pro Met Ala Asp Asn Asn Glu Gly Ala Asp Gly Val Gly Ser
210 215 220
Ser Ser Gly Asn Trp His Cys Asp Ser Thr Trp Leu Gly Asp Arg Val
225 230 235 240
Ile Thr Thr Ser Thr Arg Thr Trp Ala Leu Pro Thr Tyr Asn Asn His
245 250 255
Leu Tyr Lys Gln Ile Ser Asn Gly Thr Ser Gly Gly Ser Thr Asn Asp
260 265 270
Asn Thr Tyr Phe Gly Tyr Ser Thr Pro Trp Gly Tyr Phe Asp Phe Asn
275 280 285
Arg Phe His Cys His Phe Ser Pro Arg Asp Trp Gln Arg Leu Ile Asn
290 295 300
Asn Asn Trp Gly Phe Arg Pro Lys Arg Leu Ser Phe Lys Leu Phe Asn
305 310 315 320
Ile Gln Val Lys Glu Val Thr Gln Asp Glu Gly Thr Lys Thr Ile Ala
325 330 335
Asn Asn Leu Thr Ser Thr Ile Gln Val Phe Thr Asp Ser Glu Tyr Gln
340 345 350
Leu Pro Tyr Val Leu Gly Ser Ala His Gln Gly Cys Leu Pro Pro Phe
355 360 365
Pro Ala Asp Val Phe Met Ile Pro Gln Tyr Gly Tyr Leu Thr Leu Asn
370 375 380
Asn Gly Ser Gln Ala Val Gly Arg Ser Ser Phe Tyr Cys Leu Glu Tyr
385 390 395 400
Phe Pro Ser Gln Met Leu Arg Thr Gly Asn Asn Phe Glu Phe Ser Tyr
405 410 415
Thr Phe Glu Asp Val Pro Phe His Ser Ser Tyr Ala His Ser Gln Ser
420 425 430
Leu Asp Arg Leu Met Asn Pro Leu Ile Asp Gln Tyr Leu Tyr Tyr Leu
435 440 445
Ser Arg Thr Gln Ser Thr Gly Gly Thr Gln Gly Thr Gln Gln Leu Leu
450 455 460
Phe Ser Gln Ala Gly Pro Ala Asn Met Ser Ala Arg Ala Lys Asn Trp
465 470 475 480
Leu Pro Gly Pro Cys Tyr Arg Gln Gln Arg Val Ser Thr Thr Leu Ser
485 490 495
Gln Asn Asn Asn Ser Asn Phe Ala Trp Thr Gly Ala Thr Lys Tyr His
500 505 510
Leu Asn Gly Arg Asp Ser Leu Val Asn Pro Gly Val Ala Met Ala Thr
515 520 525
His Lys Asp Asp Glu Glu Arg Phe Phe Pro Ser Ser Gly Val Leu Met
530 535 540
Phe Gly Lys Gln Gly Ala Gly Arg Asp Asn Val Asp Tyr Ser Ser Val
545 550 555 560
Met Leu Thr Ser Glu Glu Glu Ile Lys Thr Thr Asn Pro Val Ala Thr
565 570 575
Glu Gln Tyr Gly Val Val Ala Asp Asn Leu Gln Gln Ala Asn Thr Gly
580 585 590
Pro Ile Val Gly Asn Val Asn Ser Gln Gly Ala Leu Pro Gly Met Val
595 600 605
Trp Gln Asn Arg Asp Val Tyr Leu Gln Gly Pro Ile Trp Ala Lys Ile
610 615 620
Pro His Thr Asp Gly Asn Phe His Pro Ser Pro Leu Met Gly Gly Phe
625 630 635 640
Gly Leu Lys His Pro Pro Pro Gln Ile Leu Ile Lys Asn Thr Pro Val
645 650 655
Pro Ala Asp Pro Pro Thr Thr Phe Ser Gln Ala Lys Leu Ala Ser Phe
660 665 670
Ile Thr Gln Tyr Ser Thr Gly Gln Val Ser Val Glu Ile Glu Trp Glu
675 680 685
Leu Gln Lys Glu Asn Ser Lys Arg Trp Asn Pro Glu Ile Gln Tyr Thr
690 695 700
Ser Asn Tyr Tyr Lys Ser Thr Asn Val Asp Phe Ala Val Asn Ser Glu
705 710 715 720
Gly Thr Tyr Ser Glu Pro Arg Pro Ile Gly Thr Arg Tyr Leu Thr Arg
725 730 735
Asn Leu
<210>93
<211>731
<212>PRT
<213>vp1,克隆pi.1
<400>93
Met Ala Ala Asp Gly Tyr Leu Pro Asp Trp Leu Glu Asp Asn Leu Ser
1 5 10 15
Glu Gly Ile Arg Glu Trp Trp Ala Leu Lys Pro Gly Ala Pro Gln Pro
20 25 30
Lys Ala Asn Gln Gln Lys Gln Asp Asp Gly Arg Gly Leu Val Leu Pro
35 40 45
Gly Tyr Lys Tyr Leu Gly Pro Phe Asn Gly Leu Asp Lys Gly Glu Pro
50 55 60
Val Asn Glu Ala Asp Ala Ala Ala Leu Glu His Asp Lys Ala Tyr Asp
65 70 75 80
Gln Gln Leu Lys Ala Gly Asp Asn Pro Tyr Leu Arg Tyr Asn His Ala
85 90 95
Asp Ala Glu Phe Gln Glu Arg Leu Gln Glu Asp Thr Ser Phe Gly Gly
100 105 110
Asn Leu Gly Arg Ala Val Phe Gln Ala Lys Lys Arg Val Leu Glu Pro
115 120 125
Leu Gly Leu Val Glu Glu Gly Ala Lys Thr Ala Pro Gly Lys Lys Arg
130 135 140
Pro Val Glu Pro Asp Ser Ser Ser Gly Ile Gly Lys Ser Gly Gln Gln
145 150 155 160
Pro Ala Lys Lys Arg Leu Asn Phe Gly Gln Thr Gly Asp Ser Glu Ser
165 170 175
Val Pro Asp Pro Gln Pro Leu Ser Glu Pro Pro Ala Gly Pro Ser Gly
180 185 190
Leu Gly Ser Gly Thr Met Ala Ala Gly Gly Gly Ala Pro Met Ala Asp
195 200 205
Asn Asn Glu Gly Ala Asp Gly Val Gly Asn Val Ser Gly Asn Trp His
210 215 220
Cys Asp Ser Thr Trp Leu Gly Asp Arg Val Ile Thr Thr Ser Thr Arg
225 230 235 240
Thr Trp Ala Leu Pro Thr Tyr Asn Asn His Leu Tyr Lys Gln Ile Ser
245 250 255
Asn Gly Thr Ser Gly Gly Ser Ser Asn Asp Asn Thr Tyr Phe Gly Tyr
260 265 270
Ser Thr Pro Trp Gly Tyr Phe Asp Phe Asn Arg Phe His Cys His Phe
275 280 285
Ser Pro Arg Asp Trp Gln Arg Leu Ile Asn Asn Asn Trp Gly Phe Arg
290 295 300
Pro Lys Lys Leu Asn Phe Lys Leu Phe Asn Ile Gln Val Lys Glu Val
305 310 315 320
Thr Gln Asn Glu Gly Thr Lys Thr Ile Ala Asn Asn Leu Thr Ser Thr
325 330 335
Val Gln Val Phe Thr Asp Ser Glu Tyr Gln Leu Pro Tyr Val Leu Gly
340 345 350
Ser Ala His Gln Gly Cys Leu Pro Pro Phe Pro Ala Asp Val Phe Met
355 360 365
Ile Pro Gln Tyr Gly Tyr Leu Thr Leu Asn Asn Gly Ser Gln Ala Val
370 375 380
Gly Arg Ser Ser Phe Tyr Cys Leu Glu Tyr Phe Pro Ser Gln Met Leu
385 390 395 400
Arg Thr Gly Asn Asn Phe Thr Phe Ser Tyr Thr Phe Glu Asp Val Pro
405 410 415
Phe His Ser Ser Tyr Ala His Ser Gln Ser Leu Asp Arg Leu Met Asn
420 425 430
Pro Leu Ile Asp Gln Tyr Leu Tyr Tyr Leu Ser Arg Thr Gln Thr Asn
435 440 445
Gly Thr Asn Ala Thr Gln Thr Leu Leu Phe Ala Gln Ala Gly Pro Gln
450 455 460
Asn Met Ser Ala Gln Ala Lys Asn Trp Leu Pro Gly Pro Cys Tyr Arg
465 470 475 480
Gln Gln Arg Val Ser Thr Thr Val Ser Gln Asn Asn Asn Ser Asn Phe
485 490 495
Thr Trp Thr Gly Ala Thr Lys Tyr His Leu Asn Gly Arg Asp Ser Leu
500 505 510
Val Ser Pro Gly Val Ala Met Ala Thr His Lys Asp Asp Glu Glu Arg
515 520 525
Phe Phe Pro Ser Ser Gly Val Leu Met Phe Gly Lys Gln Gly Ala Gly
530 535 540
Lys Asp Asn Val Glu Tyr Thr Asn Val Met Leu Thr Ser Glu Glu Glu
545 550 555 560
Ile Lys Thr Thr Asn Pro Val Ala Thr Glu Gln Tyr Gly Val Val Ala
565 570 575
Asp Asn Leu Gln Gln Thr Asn Ser Ala Pro Ile Val Gly Ala Val Asn
580 585 590
Ser Gln Gly Ala Leu Pro Gly Met Val Trp Gln Asn Arg Asp Val Tyr
595 600 605
Leu Gln Gly Pro Ile Trp Ala Lys Ile Pro His Thr Asp Gly Asn Phe
610 615 620
His Pro Ser Pro Leu Met Gly Gly Phe Gly Leu Lys His Pro Pro Pro
625 630 635 640
Gln Ile Leu Ile Lys Asn Thr Pro Val Pro Ala Asp Pro Pro Val Asn
645 650 655
Phe Thr Asp Ala Lys Leu Ala Ser Phe Ile Thr Gln Tyr Ser Thr Gly
660 665 670
Gln Val Ser Val Glu Ile Glu Trp Glu Leu Gln Lys Glu Asn Ser Lys
675 680 685
Arg Trp Asn Pro Glu Ile Gln Tyr Thr Ser Asn Tyr Tyr Lys Ser Ala
690 695 700
Asn Val Asp Phe Ala Val Asn Ala Asp Gly Val Tyr Ser Glu Pro Arg
705 710 715 720
Pro Ile Gly Thr Arg Tyr Leu Thr Arg Asn Leu
725 730
<210>94
<211>731
<212>PRT
<213>vp1,克隆pi.3
<400>94
Met Ala Ala Asp Gly Tyr Leu Pro Asp Trp Leu Glu Asp Asn Leu Ser
1 5 10 15
Glu Gly Ile Arg Glu Trp Trp Ala Leu Lys Pro Gly Ala Pro Gln Pro
20 25 30
Lys Ala Asn Gln Gln Lys Gln Asp Asp Gly Arg Gly Leu Val Leu Pro
35 40 45
Gly Tyr Lys Tyr Leu Gly Pro Phe Asn Gly Leu Asp Lys Gly Glu Pro
50 55 60
Val Asn Glu Ala Asp Ala Ala Ala Leu Glu His Asp Lys Ala Tyr Asp
65 70 75 80
Gln Gln Leu Lys Ala Gly Asp Asn Pro Tyr Leu Arg Tyr Asn His Ala
85 90 95
Asp Ala Glu Phe Gln Glu Arg Leu Gln Glu Asp Thr Ser Phe Gly Gly
100 105 110
Asn Leu Gly Arg Ala Val Phe Gln Ala Lys Lys Arg Val Leu Glu Pro
115 120 125
Leu Gly Leu Val Glu Glu Gly Ala Lys Thr Ala Pro Gly Lys Lys Arg
130 135 140
Pro Val Glu Pro Asp Ser Ser Ser Gly Ile Gly Lys Ser Gly Gln Gln
145 150 155 160
Pro Ala Lys Lys Arg Leu Asn Phe Gly Pro Thr Gly Asp Ser Glu Ser
165 170 175
Val Pro Asp Pro Gln Pro Leu Ser Glu Pro Pro Ala Gly Pro Ser Gly
180 185 190
Leu Gly Ser Gly Thr Met Ala Ala Gly Gly Gly Ala Pro Met Ala Asp
195 200 205
Asn Asn Glu Gly Ala Asp Gly Val Gly Asn Val Ser Gly Asn Trp His
210 215 220
Cys Asp Ser Thr Trp Leu Gly Asp Arg Val Ile Thr Thr Ser Thr Arg
225 230 235 240
Thr Trp Ala Leu Pro Thr Tyr Asn Asn His Leu Tyr Lys Gln Ile Ser
245 250 255
Asn Gly Thr Ser Gly Gly Ser Ser Asn Asp Asn Thr Tyr Phe Gly Tyr
260 265 270
Ser Thr Pro Trp Gly Tyr Phe Asp Phe Asn Arg Phe His Cys His Phe
275 280 285
Ser Pro Arg Asp Trp Gln Arg Leu Ile Asn Asn Asn Trp Gly Phe Arg
290 295 300
Pro Lys Lys Leu Asn Phe Lys Leu Phe Asn Ile Gln Val Lys Glu Val
305 310 315 320
Thr Gln Asn Glu Gly Thr Lys Thr Thr Ala Asn Asn Leu Thr Ser Thr
325 330 335
Val Gln Val Phe Thr Asp Ser Glu Tyr Gln Leu Pro Tyr Val Leu Gly
340 345 350
Ser Ala His Gln Gly Cys Leu Pro Pro Phe Pro Ala Asp Val Phe Met
355 360 365
Ile Pro Gln Tyr Gly Tyr Leu Thr Leu Asn Asn Gly Ser Gln Ala Val
370 375 380
Gly Arg Ser Ser Phe Tyr Cys Leu Glu Tyr Phe Pro Ser Gln Met Leu
385 390 395 400
Arg Thr Gly Asn Asn Phe Thr Phe Ser Tyr Thr Phe Glu Asp Val Pro
405 410 415
Phe His Ser Ser Tyr Ala His Ser Gln Ser Leu Asp Arg Leu Met Asn
420 425 430
Pro Leu Ile Asp Gln Tyr Leu Tyr Tyr Leu Ser Arg Thr Gln Thr Asn
435 440 445
Gly Thr Asn Ala Thr Gln Thr Leu Leu Phe Ala Gln Ala Gly Pro Gln
450 455 460
Asn Met Ser Ala Gln Ala Lys Asn Trp Leu Pro Gly Pro Cys Tyr Arg
465 470 475 480
Gln Gln Arg Val Ser Thr Ala Val Ser Gln Asn Asn Asn Ser Asn Phe
485 490 495
Thr Trp Thr Gly Ala Thr Lys Tyr His Leu Asn Gly Arg Asp Ser Leu
500 505 510
Val Asn Pro Gly Val Ala Met Ala Thr His Lys Asp Asp Glu Glu Arg
515 520 525
Phe Phe Pro Ser Ser Gly Val Leu Met Phe Gly Lys Gln Gly Ala Gly
530 535 540
Lys Asp Asn Val Glu Tyr Thr Asn Val Met Leu Thr Ser Glu Glu Glu
545 550 555 560
Ile Lys Thr Thr Asn Pro Val Ala Thr Glu Gln Tyr Gly Val Val Ala
565 570 575
Asp Asn Leu Gln Gln Thr Asn Ser Ala Pro Ile Val Gly Ala Val Asn
580 585 590
Ser Gln Gly Ala Leu Pro Gly Met Val Trp Gln Asn Arg Asp Val Tyr
595 600 605
Leu Gln Gly Pro Ile Trp Ala Lys Ile Pro His Thr Asp Gly Asn Phe
610 615 620
His Pro Ser Pro Leu Met Gly Gly Phe Gly Leu Lys His Pro Pro Pro
625 630 635 640
Gln Ile Leu Ile Lys Asn Thr Pro Val Pro Ala Asp Pro Pro Val Asn
645 650 655
Phe Thr Asp Ala Lys Leu Ala Ser Phe Ile Thr Gln Tyr Ser Thr Gly
660 665 670
Gln Val Ser Val Glu Ile Glu Trp Glu Leu Gln Lys Glu Asn Ser Lys
675 680 685
Arg Trp Asn Pro Glu Ile Gln Tyr Thr Ser Asn Tyr Tyr Lys Ser Ala
690 695 700
Asn Val Asp Phe Ala Val Asn Ala Asp Gly Val Tyr Ser Glu Pro Arg
705 710 715 720
Pro Ile Gly Thr Arg Tyr Leu Thr Arg Asn Leu
725 730
<210>95
<211>731
<212>PRT
<213>vp1,克隆pi.2
<400>95
Met Ala Ala Asp Gly Tyr Leu Pro Asp Trp Leu Glu Asp Asn Leu Ser
1 5 10 15
Glu Gly Ile Arg Glu Trp Trp Ala Leu Lys Pro Gly Ala Pro Gln Pro
20 25 30
Lys Ala Asn Gln Gln Lys Gln Asp Asp Gly Arg Gly Leu Val Leu Pro
35 40 45
Gly Tyr Lys Tyr Leu Gly Pro Phe Asn Gly Leu Asp Lys Gly Glu Pro
50 55 60
Val Asn Glu Ala Asp Ala Ala Ala Leu Glu His Asp Lys Ala Tyr Asp
65 70 75 80
Gln Gln Leu Lys Ala Gly Asp Asn Pro Tyr Leu Arg Tyr Asn His Ala
85 90 95
Asp Ala Glu Phe Gln Glu Arg Leu Gln Glu Asp Thr Ser Phe Gly Gly
100 105 110
Asn Leu Gly Arg Ala Val Phe Gln Ala Lys Lys Arg Val Leu Glu Pro
115 120 125
Leu Gly Leu Val Glu Glu Gly Ala Lys Thr Ala Pro Gly Lys Lys Arg
130 135 140
Pro Val Glu Pro Asp Ser Ser Ser Gly Ile Gly Lys Ser Gly Arg Gln
145 150 155 160
Pro Ala Lys Lys Arg Leu Asn Phe Gly Gln Thr Gly Asp Ser Glu Ser
165 170 175
Val Pro Asp Pro Gln Pro Leu Ser Glu Pro Pro Ala Gly Pro Ser Gly
180 185 190
Leu Gly Ser Gly Thr Met Ala Ala Gly Gly Gly Ala Pro Met Ala Asp
195 200 205
Asn Asn Glu Gly Ala Asp Gly Val Gly Asn Ala Ser Gly Asn Trp His
210 215 220
Cys Asp Ser Thr Trp Leu Gly Asp Arg Val Ile Thr Thr Ser Thr Arg
225 230 235 240
Thr Trp Ala Leu Pro Thr Tyr Asn Asn His Leu Tyr Lys Gln Ile Ser
245 250 255
Asn Gly Thr Ser Gly Gly Ser Ser Asn Asp Asn Thr Tyr Phe Gly Tyr
260 265 270
Ser Thr Pro Trp Gly Tyr Phe Asp Phe Asn Arg Phe His Cys His Phe
275 280 285
Ser Pro Arg Asp Trp Gln Arg Leu Ile Asn Asn Asn Trp Gly Phe Arg
290 295 300
Pro Lys Arg Leu Asn Phe Lys Leu Phe Asn Ile Gln Val Lys Glu Val
305 310 315 320
Thr Gln Asn Glu Gly Thr Lys Thr Ile Ala Asn Asn Leu Thr Ser Thr
325 330 335
Val Gln Val Phe Thr Asp Ser Lys Tyr Gln Leu Pro Tyr Val Leu Gly
340 345 350
Ser Ala His Gln Gly Cys Leu Pro Pro Phe Pro Ala Asp Val Phe Met
355 360 365
Ile Pro Gln Tyr Gly Tyr Leu Thr Leu Asn Asn Gly Ser Gln Ala Val
370 375 380
Gly Arg Ser Ser Phe Tyr Cys Leu Glu Tyr Phe Pro Ser Gln Met Leu
385 390 395 400
Arg Thr Gly Asn Asn Phe Thr Phe Ser Tyr Thr Phe Glu Asp Val Pro
405 410 415
Phe His Ser Ser Tyr Ala His Ser Gln Ser Leu Asp Arg Leu Met Asn
420 425 430
Pro Leu Ile Asp Gln Tyr Leu Tyr Tyr Leu Ser Arg Thr Gln Thr Asn
435 440 445
Gly Thr Asn Ala Thr Gln Thr Leu Leu Phe Ala Gln Ala Gly Pro Gln
450 455 460
Asn Met Ser Ala Gln Ala Lys Asn Trp Leu Pro Gly Pro Cys Tyr Arg
465 470 475 480
Gln Gln Arg Val Ser Thr Thr Val Ser Gln Asn Asn Asn Ser Asn Phe
485 490 495
Thr Trp Thr Gly Ala Thr Lys Tyr His Leu Asn Gly Arg Asp Ser Leu
500 505 510
Val Asn Pro Gly Val Ala Met Ala Thr His Lys Asp Asp Glu Glu Arg
515 520 525
Phe Phe Pro Ser Ser Gly Val Leu Met Phe Gly Lys Gln Gly Ala Gly
530 535 540
Lys Asp Asn Val Glu Tyr Thr Asn Val Met Leu Thr Ser Glu Glu Glu
545 550 555 560
Ile Lys Thr Thr Asn Pro Val Ala Thr Glu Gln Tyr Gly Val Val Ala
565 570 575
Asp Asn Leu Gln Gln Thr Asn Ser Ala Pro Ile Val Gly Ala Val Asn
580 585 590
Ser Gln Gly Ala Leu Pro Gly Met Val Trp Gln Asn Arg Asp Val Tyr
595 600 605
Leu Gln Gly Pro Ile Trp Ala Lys Ile Pro His Thr Asp Gly Asn Phe
610 615 620
His Pro Ser Pro Leu Met Gly Gly Phe Gly Leu Lys His Pro Pro Pro
625 630 635 640
Gln Ile Leu Ile Lys Asn Thr Pro Val Pro Ala Asp Pro Pro Val Asn
645 650 655
Phe Thr Asp Ala Lys Leu Ala Ser Phe Ile Thr Gln Tyr Ser Thr Gly
660 665 670
Gln Val Ser Val Glu Ile Glu Trp Glu Leu Gln Lys Glu Asn Ser Lys
675 680 685
Arg Trp Asn Pro Glu Ile Gln Tyr Thr Ser Asn Tyr Tyr Lys Ser Ala
690 695 700
Asn Val Asp Phe Ala Val Asn Ala Asp Gly Val Tyr Ser Glu Pro Arg
705 710 715 720
Pro Ile Gly Thr Arg Tyr Leu Thr Arg Asn Leu
725 730
<210>96
<211>738
<212>PRT
<213>vp1,克隆rh.52
<400>96
Met Ala Ala Asp Gly Tyr Leu Pro Asp Trp Leu Glu Asp Asn Leu Ser
1 5 10 15
Glu Gly Ile Arg Glu Trp Trp Asp Leu Lys Pro Gly Ala Pro Lys Pro
20 25 30
Lys Ala Asn Gln Gln Lys Gln Asp Asp Gly Arg Gly Leu Val Leu Pro
35 40 45
Gly Tyr Lys Tyr Leu Gly Pro Phe Asn Gly Leu Asp Lys Gly Glu Pro
50 55 60
Val Asn Ala Ala Asp Ala Ala Ala Leu Glu His Asp Lys Ala Tyr Asp
65 70 75 80
Gln Gln Leu Lys Ala Gly Asp Asn Pro Tyr Leu Arg Tyr Asn His Ala
85 90 95
Asp Ala Glu Phe Gln Glu Arg Leu Gln Glu Asp Thr Ser Phe Gly Gly
100 105 110
Asn Leu Gly Arg Ala Val Phe Gln Ala Lys Lys Arg Val Leu Glu Pro
115 120 125
Leu Gly Leu Val Glu Glu Gly Ala Lys Thr Ala Pro Gly Lys Lys Arg
130 135 140
Pro Val Glu Pro Ser Pro Gln Arg Ser Pro Asp Ser Ser Thr Gly Ile
145 150 155 160
Gly Lys Lys Gly Gln Gln Pro Ala Arg Lys Arg Leu Asn Phe Gly Gln
165 170 175
Thr Gly Asp Ser Glu Ser Val Pro Asp Pro Gln Pro Ile Gly Glu Pro
180 185 190
Pro Ala Ala Pro Ser Ser Val Gly Ser Gly Thr Met Ala Ala Gly Gly
195 200 205
Gly Ala Pro Met Ala Asp Asn Asn Glu Gly Ala Asp Gly Val Gly Ser
210 215 220
Ser Ser Gly Asn Trp His Cys Asp Ser Thr Trp Leu Gly Asp Arg Val
225 230 235 240
Ile Thr Thr Ser Thr Arg Thr Trp Ala Leu Pro Thr Tyr Asn Asn His
245 250 255
Leu Tyr Lys Gln Ile Ser Asn Gly Thr Ser Gly Gly Ser Thr Asn Asp
260 265 270
Asn Thr Tyr Phe Gly Tyr Ser Thr Pro Trp Gly Tyr Phe Asp Phe Asn
275 280 285
Arg Phe His Cys His Phe Ser Pro Arg Asp Trp Gln Arg Leu Ile Asn
290 295 300
Asn Asn Trp Gly Phe Arg Pro Lys Arg Leu Ser Phe Lys Leu Phe Asn
305 310 315 320
Ile Gln Val Lys Glu Val Thr Gln Asn Glu Gly Thr Lys Thr Ile Ala
325 330 335
Asn Ser Leu Thr Ser Thr Ile Gln Val Phe Thr Asp Ser Glu Tyr Gln
340 345 350
Leu Pro Tyr Val Leu Gly Ser Ala His Gln Gly Cys Leu Pro Pro Phe
355 360 365
Pro Ala Asp Val Phe Met Ile Pro Gln Tyr Gly Tyr Leu Thr Pro Asn
370 375 380
Asn Gly Ser Gln Ala Val Gly Arg Ser Ser Phe Tyr Cys Leu Glu Tyr
385 390 395 400
Phe Pro Ser Gln Met Leu Arg Thr Gly Asn Asn Phe Ser Phe Ser Tyr
405 410 415
Thr Phe Glu Asp Val Pro Phe His Ser Ser Tyr Ala His Ser Gln Ser
420 425 430
Leu Asp Arg Leu Met Asn Pro Leu Ile Asp Gln Tyr Leu Tyr Tyr Leu
435 440 445
Ser Arg Thr Gln Ser Thr Gly Gly Thr Ala Gly Thr Gln Gln Leu Leu
450 455 460
Ser Ser Gln Ala Gly Pro Ser Asn Met Ser Ala Gln Ala Arg Asn Trp
465 470 475 480
Leu Pro Gly Pro Cys Tyr Arg Gln Gln Arg Val Ser Thr Thr Leu Ser
485 490 495
Gln Asn Asn Asn Ser Asn Phe Ala Trp Thr Gly Ala Thr Lys Tyr His
500 505 510
Leu Asn Gly Arg Asp Ser Leu Val Asn Pro Gly Val Ala Met Ala Thr
515 520 525
Asn Lys Asp Asp Glu Asp Arg Phe Phe Pro Ser Ser Gly Ile Leu Met
530 535 540
Phe Gly Lys Gln Gly Ala Gly Lys Asp Asn Val Asp Tyr Ser Asn Val
545 550 555 560
Met Leu Thr Ser Glu Glu Glu Ile Lys Thr Thr Asn Pro Val Ala Thr
565 570 575
Glu Gln Tyr Gly Val Val Ala Asp Asn Leu Gln Gln Gln Asn Thr Ala
580 585 590
Pro Ile Val Gly Ala Val Asn Ser Gln Gly Ala Leu Pro Gly Met Val
595 600 605
Trp Gln Asn Arg Asp Val Tyr Leu Gln Gly Pro Ile Trp Ala Lys Ile
610 615 620
Pro His Thr Asp Gly Asn Phe His Pro Ser Pro Leu Met Gly Gly Phe
625 630 635 640
Gly Leu Lys His Pro Pro Pro Gln Ile Leu Ile Lys Asn Thr Pro Val
645 650 655
Pro Ala Asp Pro Pro Thr Ala Phe Asn Gln Ala Lys Leu Asn Ser Phe
660 665 670
Ile Thr Gln Tyr Ser Thr Gly Gln Val Ser Val Glu Ile Glu Trp Glu
675 680 685
Leu Gln Lys Glu Asn Ser Lys Arg Trp Asn Pro Glu Ile Gln Tyr Thr
690 695 700
Ser Asn Tyr Tyr Lys Ser Thr Asn Val Asp Phe Ala Val Asn Thr Glu
705 710 715 720
Gly Val Tyr Ser Glu Pro Arg Pro Ile Gly Thr Arg Tyr Leu Thr Arg
725 730 735
Asn Leu
<210>97
<211>738
<212>PRT
<213>vp1,克隆rh.53
<400>97
Met Ala Ala Asp Gly Tyr Leu Pro Asp Trp Leu Glu Asp Asn Leu Ser
1 5 10 15
Glu Gly Ile Arg Glu Trp Trp Asp Leu Lys Pro Gly Ala Pro Lys Pro
20 25 30
Lys Ala Asn Gln Gln Lys Gln Asp Asp Gly Arg Gly Leu Val Leu Pro
35 40 45
Gly Tyr Lys Tyr Leu Gly Pro Phe Asn Gly Leu Asp Lys Gly Glu Pro
50 55 60
Val Asn Ala Ala Asp Ala Ala Ala Leu Glu His Asp Lys Ala Tyr Asp
65 70 75 80
Gln Gln Leu Lys Ala Gly Asp Asn Pro Tyr Leu Arg Tyr Asn His Ala
85 90 95
Asp Ala Glu Phe Gln Glu Arg Leu Gln Glu Asp Thr Ser Phe Gly Gly
100 105 110
Asn Leu Gly Arg Ala Val Phe Gln Ala Lys Lys Arg Val Leu Glu Pro
115 120 125
Leu Gly Leu Val Glu Glu Gly Ala Lys Thr Ala Pro Gly Lys Lys Arg
130 135 140
Pro Val Glu Pro Ser Pro Gln Arg Ser Pro Asp Ser Ser Thr Gly Ile
145 150 155 160
Gly Lys Lys Gly Gln Gln Pro Ala Arg Lys Arg Leu Asn Phe Gly Gln
165 170 175
Thr Gly Asp Ser Glu Ser Val Pro Asp Pro Gln Pro Ile Gly Glu Pro
180 185 190
Pro Ala Ala Pro Ser Ser Val Gly Ser Gly Thr Met Ala Ala Gly Gly
195 200 205
Gly Ala Pro Met Ala Asp Asn Asn Glu Gly Ala Asp Gly Val Gly Ser
210 215 220
Ser Ser Gly Asn Trp His Cys Asp Ser Thr Trp Leu Gly Asp Arg Val
225 230 235 240
Ile Thr Thr Ser Thr Arg Thr Trp Ala Leu Pro Thr Tyr Asn Asn His
245 250 255
Leu Tyr Lys Gln Ile Ser Asn Gly Thr Ser Gly Gly Ser Thr Asn Asp
260 265 270
Asn Thr Tyr Phe Gly Tyr Ser Thr Pro Trp Gly Tyr Phe Asp Phe Asn
275 280 285
Arg Phe His Cys His Phe Ser Pro Arg Asp Trp Gln Arg Leu Ile Asn
290 295 300
Asn Asn Trp Gly Phe Arg Pro Lys Arg Leu Ser Phe Lys Leu Phe Asn
305 310 315 320
Ile Gln Val Lys Glu Val Thr Gln Asn Glu Gly Thr Lys Thr Ile Ala
325 330 335
Asn Asn Leu Thr Ser Thr Ile Gln Val Phe Thr Asp Ser Glu Tyr Gln
340 345 350
Leu Pro Tyr Val Leu Gly Ser Ala His Gln Gly Cys Leu Pro Pro Phe
355 360 365
Pro Ala Asp Val Phe Met Ile Pro Gln Tyr Gly Tyr Leu Thr Leu Asn
370 375 380
Asn Gly Ser Gln Ala Val Gly Arg Ser Ser Phe Tyr Cys Leu Glu Tyr
385 390 395 400
Phe Pro Ser Gln Met Leu Arg Thr Gly Asn Asn Phe Ser Phe Ser Tyr
405 410 415
Thr Phe Glu Asp Val Pro Phe His Ser Ser Tyr Val His Ser Gln Ser
420 425 430
Leu Asp Arg Leu Met Asn Pro Leu Ile Asp Gln Tyr Leu Tyr Tyr Leu
435 440 445
Ser Arg Thr Gln Ser Thr Gly Gly Thr Ala Gly Thr Gln Gln Leu Leu
450 455 460
Phe Ser Gln Ala Gly Pro Ser Asn Met Ser Ala Gln Ala Arg Asn Trp
465 470 475 480
Leu Pro Gly Pro Cys Tyr Arg Gln Gln Arg Val Ser Thr Thr Leu Ser
485 490 495
Gln Asn Asn Asn Ser Asn Phe Ala Trp Thr Gly Ala Thr Lys Tyr His
500 505 510
Leu Asn Gly Arg Asp Ser Leu Val Asn Ser Gly Val Ala Met Ala Thr
515 520 525
Asn Lys Asp Asp Glu Asp Arg Phe Phe Pro Ser Ser Gly Ile Leu Met
530 535 540
Phe Gly Lys Gln Gly Ala Gly Lys Asp Asn Val Asp Tyr Ser Asn Val
545 550 555 560
Met Leu Thr Ser Glu Glu Glu Ile Lys Thr Thr Asn Pro Val Ala Thr
565 570 575
Glu Gln Tyr Gly Val Val Ala Asp Asn Leu Gln Gln Gln Asn Thr Ala
580 585 590
Pro Ile Val Gly Ala Val Asn Ser Gln Gly Ala Leu Pro Gly Met Val
595 600 605
Trp Gln Asn Arg Asp Val Tyr Leu Gln Gly Pro Ile Trp Ala Lys Ile
610 615 620
Pro His Thr Asp Gly Asn Phe His Pro Ser Pro Leu Met Gly Gly Phe
625 630 635 640
Gly Leu Lys His Pro Pro Pro Gln Ile Leu Ile Lys Asn Thr Pro Val
645 650 655
Pro Ala Asp Pro Pro Thr Ala Phe Asn Gln Ala Lys Leu Asn Ser Phe
660 665 670
Ile Thr Gln Tyr Ser Thr Gly Gln Val Ser Val Glu Ile Glu Trp Glu
675 680 685
Leu Gln Lys Glu Asn Ser Lys Arg Trp Asn Pro Glu Ile Gln Tyr Thr
690 695 700
Ser Asn Tyr Tyr Lys Ser Thr Asn Val Asp Phe Ala Val Asn Thr Glu
705 710 715 720
Gly Val Tyr Ser Glu Pro Arg Pro Ile Gly Thr Arg Tyr Pro Thr Arg
725 730 735
Asn Leu
<210>98
<211>738
<212>PRT
<213>vp1,克隆rh.70
<400>98
Met Ala Ala Asp Gly Tyr Leu Pro Asp Trp Leu Glu Asp Asn Leu Ser
1 5 10 15
Glu Gly Ile Arg Glu Trp Trp Asp Leu Lys Pro Gly Ala Pro Lys Pro
20 25 30
Lys Ala Asn Gln Gln Lys Gln Asp Asp Gly Arg Gly Leu Val Leu Pro
35 40 45
Gly Tyr Lys Tyr Leu Gly Pro Phe Asn Gly Leu Asp Lys Gly Glu Pro
50 55 60
Val Asn Ala Ala Asp Ala Ala Ala Leu Glu His Asp Lys Ala Tyr Asp
65 70 75 80
Gln Gln Leu Lys Ala Gly Asp Asn Pro Tyr Leu Arg Tyr Asn His Ala
85 90 95
Asp Ala Glu Phe Gln Glu Arg Leu Gln Glu Asp Thr Ser Phe Gly Gly
100 105 110
Asn Leu Gly Arg Ala Val Phe Gln Ala Lys Lys Arg Val Leu Glu Pro
115 120 125
Leu Gly Leu Val Glu Glu Gly Ala Lys Thr Ala Pro Gly Lys Lys Arg
130 135 140
Pro Val Glu Pro Ser Pro Gln Arg Ser Pro Asp Ser Ser Thr Gly Ile
145 150 155 160
Gly Lys Lys Gly Gln Gln Pro Ala Arg Lys Arg Leu Asn Phe Gly Gln
165 170 175
Thr Gly Asp Ser Glu Ser Val Pro Asp Pro Gln Pro Ile Gly Glu Pro
180 185 190
Pro Ala Ala Pro Ser Ser Val Gly Ser Gly Thr Met Ala Ala Gly Gly
195 200 205
Gly Ala Pro Met Ala Asp Asn Asn Glu Gly Ala Asp Gly Val Gly Ser
210 215 220
Ser Ser Gly Asn Trp His Cys Asp Ser Thr Trp Leu Gly Asp Arg Val
225 230 235 240
Ile Thr Thr Ser Thr Arg Thr Trp Ala Leu Pro Ala Tyr Asn Asn His
245 250 255
Leu Tyr Lys Gln Ile Ser Asn Gly Thr Ser Gly Gly Ser Thr Asn Asp
260 265 270
Asn Thr Tyr Phe Gly Tyr Ser Thr Pro Trp Gly Tyr Phe Asp Phe Asn
275 280 285
Arg Phe His Cys His Phe Ser Pro Arg Asp Trp Gln Arg Leu Ile Asn
290 295 300
Asn Asn Trp Gly Phe Arg Pro Lys Arg Leu Ser Phe Lys Leu Phe Asn
305 310 315 320
Ile Gln Val Lys Glu Val Thr Gln Asn Glu Gly Thr Lys Thr Ile Ala
325 330 335
Asn Asn Leu Thr Ser Thr Ile Gln Val Phe Thr Asp Ser Glu Tyr Gln
340 345 350
Leu Pro Tyr Val Leu Gly Ser Ala His Gln Gly Cys Leu Pro Pro Phe
355 360 365
Pro Ala Asp Val Phe Met Ile Pro Gln Tyr Gly Tyr Leu Thr Leu Asn
370 375 380
Asn Gly Ser Gln Ala Val Gly Arg Ser Ser Phe Tyr Cys Leu Glu Tyr
385 390 395 400
Phe Pro Ser Gln Met Leu Arg Thr Gly Asn Asn Phe Ser Phe Ser Tyr
405 410 415
Thr Phe Glu Asp Val Pro Phe His Ser Ser Tyr Ala His Ser Gln Ser
420 425 430
Leu Asp Arg Leu Met Asn Pro Leu Ile Asp Gln Tyr Leu Tyr Tyr Leu
435 440 445
Ser Arg Thr Gln Ser Thr Gly Gly Thr Ala Gly Thr Gln Gln Leu Leu
450 455 460
Phe Ser Gln Ala Gly Pro Ser Asn Met Ser Ala Gln Ala Arg Asn Trp
465 470 475 480
Leu Pro Gly Pro Cys Tyr Arg Gln Gln Arg Val Ser Thr Thr Leu Ser
485 490 495
Gln Asn Asn Asn Ser Asn Phe Ala Trp Thr Gly Ala Thr Lys Tyr His
500 505 510
Leu Ser Gly Arg Asp Ser Leu Val Asn Pro Gly Val Ala Met Ala Thr
515 520 525
Asn Lys Asp Asp Glu Asp Arg Phe Phe Pro Ser Ser Gly Ile Leu Met
530 535 540
Phe Gly Lys Gln Gly Ala Gly Lys Asp Asn Val Asp Tyr Ser Asn Val
545 550 555 560
Met Leu Thr Ser Glu Glu Glu Ile Lys Thr Thr Asn Pro Val Ala Thr
565 570 575
Glu Gln Tyr Gly Val Val Ala Asp Asn Leu Gln Gln Gln Asn Thr Ala
580 585 590
Pro Ile Val Gly Ala Val Asn Ser Gln Gly Ala Leu Pro Gly Met Val
595 600 605
Trp Gln Asn Arg Asp Val Tyr Leu Gln Gly Pro Ile Trp Ala Lys Ile
610 615 620
Pro His Thr Asp Gly Asn Phe His Pro Ser Pro Leu Met Gly Gly Phe
625 630 635 640
Gly Leu Lys His Pro Pro Pro Gln Ile Leu Ile Lys Asn Thr Pro Val
645 650 655
Pro Ala Asp Pro Pro Thr Ala Phe Asn Gln Ala Lys Leu Asn Ser Ser
660 665 670
Ile Thr Gln Tyr Ser Thr Gly Gln Val Ser Val Glu Ile Glu Trp Glu
675 680 685
Leu Gln Lys Glu Asn Ser Lys Arg Trp Asn Pro Glu Ile Gln Tyr Thr
690 695 700
Ser Asn Tyr Tyr Lys Ser Thr Asn Val Asp Phe Ala Val Asn Thr Glu
705 710 715 720
Gly Val Tyr Ser Glu Pro Arg Pro Ile Gly Thr Arg Tyr Leu Thr Arg
725 730 735
Asn Leu
<210>99
<211>738
<212>PRT
<213>vp1,克隆rh.64
<400>99
Met Ala Ala Asp Gly Tyr Leu Pro Asp Trp Leu Glu Asp Asn Leu Ser
1 5 10 15
Glu Gly Ile Arg Glu Trp Trp Asp Leu Lys Pro Gly Ala Pro Lys Pro
20 25 30
Lys Ala Asn Gln Gln Lys Gln Asp Asp Gly Arg Gly Leu Val Leu Pro
35 40 45
Gly Tyr Lys Tyr Leu Gly Pro Phe Asn Gly Leu Asp Lys Gly Glu Pro
50 55 60
Val Asn Ala Ala Asp Ala Ala Ala Leu Glu His Asp Lys Ala Tyr Asp
65 70 75 80
Gln Gln Leu Lys Ala Gly Asp Asn Pro Tyr Leu Arg Tyr Asn His Ala
85 90 95
Asp Ala Glu Phe Gln Glu Arg Leu Gln Glu Asp Thr Ser Phe Gly Gly
100 105 110
Asn Leu Gly Arg Ala Val Phe Gln Ala Lys Lys Arg Val Leu Glu Pro
115 120 125
Leu Gly Leu Val Glu Glu Gly Ala Lys Thr Ala Pro Gly Lys Lys Arg
130 135 140
Pro Val Glu Pro Ser Pro Gln Arg Ser Pro Asp Ser Ser Thr Gly Ile
145 150 155 160
Gly Lys Lys Gly Gln Gln Pro Ala Arg Lys Arg Leu Asn Phe Gly Gln
165 170 175
Thr Gly Asp Ser Glu Ser Val Pro Asp Pro Gln Pro Ile Gly Glu Pro
180 185 190
Pro Ala Ala Pro Ser Ser Val Gly Ser Gly Thr Met Ala Ala Gly Gly
195 200 205
Gly Ala Pro Met Ala Asp Asn Asn Glu Gly Ala Asp Gly Val Gly Ser
210 215 220
Ser Ser Gly Asn Trp His Cys Asp Ser Thr Trp Leu Gly Asp Arg Val
225 230 235 240
Ile Thr Thr Ser Thr Arg Thr Trp Ala Leu Pro Thr Tyr Asn Asn His
245 250 255
Leu Tyr Lys Gln Ile Ser Asn Gly Thr Ser Gly Gly Ser Thr Asn Asp
260 265 270
Asn Thr Tyr Phe Gly Tyr Ser Thr Pro Trp Gly Tyr Phe Asp Phe Asn
275 280 285
Arg Phe His Cys His Phe Ser Pro Arg Asp Trp Gln Arg Leu Ile Asn
290 295 300
Asn Asn Trp Gly Phe Arg Pro Lys Arg Leu Ser Phe Lys Leu Phe Asn
305 310 315 320
Ile Gln Val Lys Glu Val Thr Gln Asn Glu Gly Thr Lys Thr Ile Ala
325 330 335
Asn Asn Leu Thr Ser Thr Ile Gln Val Phe Thr Asp Ser Glu Tyr Gln
340 345 350
Leu Pro Tyr Val Leu Gly Ser Ala His Gln Gly Cys Leu Pro Pro Phe
355 360 365
Pro Ala Asp Val Phe Met Ile Pro Gln Tyr Gly Tyr Leu Thr Leu Asn
370 375 380
Asn Gly Ser Gln Ala Val Gly Arg Ser Ser Phe Tyr Cys Leu Glu Tyr
385 390 395 400
Phe Pro Ser Gln Met Leu Arg Thr Gly Asn Asn Phe Ser Phe Ser Tyr
405 410 415
Thr Phe Glu Asp Val Pro Phe His Ser Ser Tyr Ala His Ser Gln Ser
420 425 430
Leu Asp Arg Leu Met Asn Pro Leu Ile Asp Gln Tyr Leu Tyr Tyr Leu
435 440 445
Ser Arg Thr Gln Ser Thr Gly Gly Thr Ala Gly Thr Gln Gln Leu Leu
450 455 460
Phe Ser Gln Ala Gly Pro Ser Asn Met Ser Ala Gln Ala Arg Asn Trp
465 470 475 480
Leu Pro Gly Pro Cys Tyr Arg Gln Gln Arg Val Ser Thr Thr Leu Ser
485 490 495
Gln Asn Asn Asn Ser Asn Phe Ala Trp Thr Gly Ala Thr Lys Tyr His
500 505 510
Leu Asn Gly Arg Asp Ser Leu Val Asn Pro Gly Val Ala Met Ala Thr
515 520 525
Asn Lys Asp Asp Glu Asp Arg Phe Phe Pro Ser Ser Gly Ile Leu Met
530 535 540
Phe Gly Lys Gln Gly Ala Gly Lys Asp Asn Val Asp Tyr Ser Asn Val
545 550 555 560
Met Leu Thr Ser Glu Glu Glu Ile Lys Thr Thr Asn Pro Val Ala Thr
565 570 575
Glu Gln Tyr Gly Val Val Ala Asp Asn Leu Gln Gln Gln Asn Thr Ala
580 585 590
Pro Ile Val Gly Ala Val Asn Ser Gln Gly Ala Leu Pro Gly Met Val
595 600 605
Trp Gln Asn Arg Asp Val Tyr Leu Gln Gly Pro Ile Trp Ala Lys Ile
610 615 620
Pro His Thr Asp Gly Asn Phe His Pro Ser Pro Leu Met Gly Gly Phe
625 630 635 640
Gly Leu Lys His Pro Pro Pro Gln Ile Leu Ile Lys Asn Thr Pro Val
645 650 655
Pro Ala Asp Pro Pro Thr Ala Phe Asn Gln Ala Lys Leu Asn Ser Phe
660 665 670
Ile Thr Gln Tyr Ser Thr Gly Gln Val Ser Val Glu Ile Val Trp Glu
675 680 685
Leu Gln Lys Glu Asn Ser Lys Arg Arg Asn Pro Glu Ile Gln Tyr Thr
690 695 700
Ser Asn Tyr Tyr Lys Ser Thr Asn Val Asp Phe Ala Val Asn Thr Glu
705 710 715 720
Gly Val Tyr Ser Glu Pro Arg Pro Ile Gly Thr Arg Tyr Leu Thr Arg
725 730 735
Asn Leu
<210>100
<211>738
<212>PRT
<213>vp1,克隆rh.68
<400>100
Met Ala Ala Asp Gly Tyr Leu Pro Asp Trp Leu Glu Asp Asn Leu Ser
1 5 10 15
Glu Gly Ile Arg Glu Trp Trp Asp Leu Lys Pro Gly Ala Pro Lys Pro
20 25 30
Lys Ala Asn Gln Gln Lys Gln Asp Asp Gly Arg Gly Leu Val Leu Pro
35 40 45
Gly Tyr Lys Tyr Leu Gly Pro Phe Asn Gly Leu Asp Lys Gly Glu Pro
50 55 60
Val Asn Ala Ala Asp Ala Ala Ala Leu Glu His Asp Lys Ala Tyr Asp
65 70 75 80
Gln Gln Leu Lys Ala Gly Asp Asn Pro Tyr Leu Arg Tyr Asn His Ala
85 90 95
Asp Ala Glu Phe Gln Glu Arg Leu Gln Glu Asp Thr Ser Phe Gly Gly
100 105 110
Asn Leu Gly Arg Ala Val Phe Gln Ala Lys Lys Arg Val Leu Glu Pro
115 120 125
Leu Gly Leu Val Glu Glu Gly Ala Lys Thr Ala Pro Gly Lys Lys Arg
130 135 140
Pro Val Glu Pro Ser Pro Gln Arg Ser Pro Asp Ser Ser Thr Gly Ile
145 150 155 160
Gly Lys Lys Gly Gln Gln Pro Ala Arg Lys Arg Leu Asn Phe Gly Gln
165 170 175
Thr Gly Asp Ser Glu Ser Val Pro Asp Pro Gln Pro Ile Gly Glu Pro
180 185 190
Pro Ala Ala Pro Ser Ser Val Gly Ser Gly Thr Met Ala Ala Gly Gly
195 200 205
Gly Ala Pro Met Ala Asp Asn Asn Glu Gly Ala Asp Gly Val Gly Ser
210 215 220
Ser Ser Gly Asn Trp His Cys Asp Ser Thr Trp Leu Gly Asp Arg Val
225 230 235 240
Ile Thr Thr Ser Thr Arg Thr Trp Ala Leu Pro Thr Tyr Asn Asn His
245 250 255
Leu Tyr Lys Gln Ile Ser Asn Gly Thr Ser Gly Gly Ser Thr Asn Asp
260 265 270
Asn Thr Tyr Phe Gly Tyr Ser Thr Pro Trp Gly Tyr Phe Asp Phe Asn
275 280 285
Arg Phe His Cys His Phe Ser Pro Arg Asp Trp Gln Arg Leu Ile Asn
290 295 300
Asn Asn Trp Gly Phe Arg Pro Lys Arg Leu Ser Phe Lys Leu Phe Asn
305 310 315 320
Ile Gln Val Lys Glu Val Thr Gln Asn Glu Gly Thr Lys Thr Ile Ala
325 330 335
Asn Asn Leu Thr Ser Thr Ile Gln Val Phe Thr Asp Ser Glu Tyr Gln
340 345 350
Leu Pro Tyr Val Leu Gly Ser Ala His Gln Gly Cys Leu Pro Pro Phe
355 360 365
Pro Ala Asp Val Phe Met Ile Pro Gln Tyr Gly Tyr Leu Thr Leu Asn
370 375 380
Asn Gly Ser Gln Ala Val Gly Arg Ser Ser Phe Tyr Cys Leu Glu Tyr
385 390 395 400
Phe Pro Ser Gln Met Leu Arg Thr Gly Asn Asn Phe Ser Phe Ser Tyr
405 410 415
Thr Phe Glu Asp Val Pro Phe His Ser Ser Tyr Ala His Ser Gln Ser
420 425 430
Leu Asp Arg Leu Met Asn Pro Leu Ile Asp Gln Tyr Leu Tyr Tyr Leu
435 440 445
Ser Arg Thr Gln Ser Thr Gly Gly Thr Ala Gly Thr Gln Gln Leu Leu
450 455 460
Phe Ser Gln Ala Gly Pro Ser Asn Met Ser Ala Gln Ala Arg Asn Trp
465 470 475 480
Leu Pro Gly Pro Cys Tyr Arg Gln Gln Arg Val Ser Thr Thr Leu Ser
485 490 495
Gln Asn Asn Asn Ser Asn Phe Ala Trp Thr Gly Ala Thr Lys Tyr His
500 505 510
Leu Asn Gly Arg Asp Ser Leu Val Asn Pro Gly Val Ala Met Ala Thr
515 520 525
Asn Lys Asp Asp Glu Asp Arg Phe Phe Pro Pro Ser Gly Ile Leu Met
530 535 540
Phe Gly Lys Gln Gly Ala Gly Lys Asp Asn Val Asp Tyr Ser Asn Val
545 550 555 560
Met Leu Thr Ser Glu Glu Glu Ile Lys Thr Thr Asn Pro Val Ala Thr
565 570 575
Glu Gln Tyr Gly Val Val Ala Asp Asn Leu Gln Gln Gln Asn Thr Ala
580 585 590
Pro Ile Val Gly Ala Val Asn Ser Gln Gly Ala Leu Pro Gly Met Val
595 600 605
Trp Gln Asn Arg Asp Val Tyr Leu Gln Gly Pro Ile Trp Ala Lys Ile
610 615 620
Pro His Thr Asp Gly Asn Phe His Pro Ser Pro Leu Met Gly Gly Phe
625 630 635 640
Gly Leu Lys His Pro Pro Pro Gln Ile Leu Ile Lys Asn Thr Pro Val
645 650 655
Pro Ala Asp Pro Pro Thr Ala Phe Asn Gln Ala Lys Leu Asn Ser Phe
660 665 670
Ile Thr Gln Tyr Ser Thr Gly Gln Val Ser Val Val Ile Glu Trp Glu
675 680 685
Leu Gln Lys Glu Asn Ser Lys Arg Trp Asn Pro Glu Ile Gln Tyr Thr
690 695 700
Ser Asn Tyr Tyr Lys Ser Thr Asn Val Asp Phe Ala Val Asn Thr Glu
705 710 715 720
Gly Val Tyr Ser Glu Leu Arg Pro Ile Gly Thr Arg Tyr Leu Thr Arg
725 730 735
Asn Leu
<210>101
<211>738
<212>PRT
<213>vp1,克隆rh.46
<400>101
Met Ala Ala Asp Gly Tyr Leu Pro Asp Trp Leu Glu Asp Asn Leu Ser
1 5 10 15
Glu Gly Ile Arg Glu Trp Trp Asp Leu Lys Pro Gly Ala Pro Lys Pro
20 25 30
Lys Ala Asn Gln Gln Lys Gln Asp Asp Gly Arg Gly Leu Val Leu Pro
35 40 45
Gly Tyr Lys Tyr Leu Gly Pro Phe Asn Gly Leu Asp Lys Gly Glu Pro
50 55 60
Val Asn Ala Ala Asp Ala Ala Ala Leu Glu His Asp Lys Ala Tyr Asp
65 70 75 80
Gln Gln Leu Lys Ala Gly Asp Asn Pro Tyr Leu Arg Tyr Asn His Ala
85 90 95
Asp Ala Glu Phe Gln Glu Arg Leu Gln Glu Asp Thr Ser Phe Gly Gly
100 105 110
Asn Leu Gly Arg Ala Val Phe Gln Ala Lys Lys Arg Val Leu Glu Pro
115 120 125
Leu Gly Leu Val Glu Glu Gly Ala Lys Thr Ala Pro Gly Lys Lys Arg
130 135 140
Pro Val Glu Pro Ser Pro Gln Arg Ser Pro Asp Ser Ser Thr Gly Ile
145 150 155 160
Gly Lys Lys Gly Gln Gln Pro Ala Arg Lys Arg Leu Asn Phe Gly Gln
165 170 175
Thr Gly Asp Ser Glu Ser Val Pro Asp Pro Gln Pro Ile Gly Glu Pro
180 185 190
Pro Ala Ala Pro Ser Ser Val Gly Ser Gly Thr Met Ala Ala Gly Gly
195 200 205
Gly Ala Pro Met Ala Asp Asn Asn Glu Gly Ala Asp Gly Val Gly Ser
210 215 220
Ser Ser Gly Asn Trp His Cys Asp Ser Thr Trp Leu Gly Asp Arg Val
225 230 235 240
Ile Thr Thr Ser Thr Arg Thr Trp Ala Leu Pro Thr Tyr Asn Asn His
245 250 255
Leu Tyr Lys Gln Ile Ser Asn Gly Thr Ser Gly Gly Ser Thr Asn Asp
260 265 270
Asn Thr Tyr Phe Gly Tyr Ser Thr Pro Trp Gly Tyr Phe Asp Phe Asn
275 280 285
Arg Phe His Cys His Phe Ser Pro Arg Asp Trp Gln Arg Leu Ile Asn
290 295 300
Asn Asn Trp Gly Phe Arg Pro Lys Arg Leu Ser Phe Lys Leu Phe Asn
305 310 315 320
Ile Gln Val Lys Glu Val Thr Gln Asn Glu Gly Thr Lys Thr Ile Ala
325 330 335
Asn Asn Leu Thr Ser Thr Ile Gln Val Phe Thr Asp Ser Glu Tyr Gln
340 345 350
Leu Pro Tyr Val Leu Gly Ser Ala His Gln Gly Cys Leu Pro Pro Phe
355 360 365
Pro Ala Asp Val Phe Met Ile Pro Gln Tyr Gly Tyr Leu Thr Leu Asn
370 375 380
Asn Gly Ser Gln Ala Val Gly Arg Ser Ser Phe Tyr Cys Leu Glu Tyr
385 390 395 400
Phe Pro Ser Gln Met Leu Arg Thr Gly Asn Asn Phe Ser Phe Ser Tyr
405 410 415
Thr Phe Glu Asp Val Pro Phe His Ser Ser Tyr Ala His Ser Gln Ser
420 425 430
Leu Asp Arg Leu Met Asn Pro Leu Ile Asp Gln Tyr Leu Tyr Tyr Leu
435 440 445
Ser Arg Thr Gln Ser Thr Gly Gly Thr Ala Gly Thr Gln Gln Leu Leu
450 455 460
Phe Ser Gln Ala Gly Pro Ser Asn Met Ser Ala Gln Ala Arg Asn Trp
465 470 475 480
Leu Pro Gly Pro Cys Tyr Arg Gln Gln Arg Val Ser Thr Thr Leu Ser
485 490 495
Gln Asn Asn Asn Ser Asn Phe Ala Trp Thr Gly Ala Thr Lys Tyr His
500 505 510
Leu Asn Gly Arg Asp Ser Leu Val Asn Pro Gly Val Ala Met Ala Thr
515 520 525
Asn Lys Asp Asp Glu Asp Arg Phe Phe Pro Ser Ser Gly Ile Leu Met
530 535 540
Phe Gly Lys Gln Gly Ala Gly Lys Asp Asn Val Asp Tyr Ser Asn Val
545 550 555 560
Met Leu Thr Ser Glu Glu Glu Ile Lys Ala Thr Asn Pro Val Ala Thr
565 570 575
Glu Gln Tyr Gly Val Val Ala Asp Asn Leu Gln Gln Gln Asn Thr Ala
580 585 590
Pro Ile Val Gly Ala Val Asn Ser Gln Gly Ala Leu Pro Gly Met Val
595 600 605
Trp Gln Asn Arg Asp Val Tyr Leu Gln Gly Pro Ile Trp Ala Lys Ile
610 615 620
Pro His Thr Asp Gly Asn Phe His Pro Ser Pro Leu Met Gly Gly Phe
625 630 635 640
Gly Leu Lys His Pro Pro Pro Gln Ile Leu Ile Lys Asn Thr Pro Val
645 650 655
Pro Ala Asp Pro Pro Thr Ala Phe Asn Gln Ala Lys Leu Asn Ser Phe
660 665 670
Ile Thr Gln Tyr Ser Thr Gly Gln Val Ser Val Glu Ile Glu Trp Glu
675 680 685
Leu Gln Lys Glu Asn Ser Lys Arg Trp Asn Pro Glu Ile Gln Tyr Thr
690 695 700
Ser Asn Tyr Tyr Lys Ser Thr Asn Val Asp Phe Ala Val Asn Thr Glu
705 710 715 720
Gly Val Tyr Ser Glu Pro Arg Pro Ile Gly Thr Arg Tyr Leu Thr Arg
725 730 735
Asn Leu
<210>102
<211>738
<212>PRT
<213>vp1,克隆hu.39
<400>102
Met Ala Ala Asp Gly Tyr Leu Pro Asp Trp Leu Glu Asp Asn Leu Ser
1 5 10 15
Glu Gly Ile Arg Glu Trp Trp Asp Leu Lys Pro Gly Ala Pro Lys Pro
20 25 30
Lys Ala Asn Gln Gln Lys Gln Asp Asp Gly Arg Gly Leu Val Leu Pro
35 40 45
Gly Tyr Lys Tyr Leu Gly Pro Phe Asn Gly Leu Asp Lys Gly Glu Pro
50 55 60
Val Asn Ala Ala Asp Ala Ala Ala Leu Glu His Asp Lys Ala Tyr Asp
65 70 75 80
Gln Gln Leu Lys Ala Gly Asp Asn Pro Tyr Leu Arg Tyr Asn His Ala
85 90 95
Asp Ala Glu Phe Gln Glu Arg Leu Gln Glu Asp Thr Ser Phe Gly Gly
100 105 110
Asn Leu Gly Arg Ala Val Phe Gln Ala Lys Lys Arg Val Leu Glu Pro
115 120 125
Leu Gly Leu Val Glu Glu Gly Ala Lys Thr Ala Pro Gly Lys Lys Arg
130 135 140
Pro Val Glu Pro Ser Pro Gln Arg Ser Pro Asp Ser Ser Thr Gly Ile
145 150 155 160
Gly Lys Lys Gly Gln Gln Pro Ala Arg Lys Arg Leu Asn Phe Gly Arg
165 170 175
Thr Gly Asp Ser Glu Ser Val Pro Asp Pro Gln Pro Ile Gly Glu Pro
180 185 190
Pro Ala Ala Pro Ser Ser Val Gly Ser Gly Thr Met Ala Ala Gly Gly
195 200 205
Gly Ala Pro Met Ala Asp Asn Asn Glu Gly Ala Asp Gly Val Gly Ser
210 215 220
Ser Ser Gly Asn Trp His Cys Asp Ser Thr Trp Leu Gly Asp Arg Val
225 230 235 240
Ile Thr Thr Ser Thr Arg Thr Trp Ala Leu Pro Thr Tyr Asn Asn His
245 250 255
Leu Tyr Lys Gln Ile Ser Asn Gly Thr Ser Gly Gly Ser Thr Asn Asp
260 265 270
Asn Thr Tyr Phe Gly Tyr Ser Thr Pro Trp Gly Tyr Leu Asp Phe Asn
275 280 285
Arg Phe His Cys His Phe Ser Pro Arg Asp Trp Gln Arg Leu Ile Asn
290 295 300
Asn Asn Trp Gly Phe Arg Pro Lys Arg Leu Ser Phe Lys Leu Phe Asn
305 310 315 320
Ile Gln Val Lys Glu Val Thr Gln Asn Glu Gly Thr Lys Thr Ile Ala
325 330 335
Asn Asn Leu Ala Ser Thr Ile Gln Val Phe Thr Asp Ser Glu Tyr Gln
340 345 350
Pro Pro Tyr Val Leu Gly Ser Ala His Gln Gly Cys Leu Pro Pro Phe
355 360 365
Pro Ala Asp Val Phe Met Ile Pro Gln Tyr Gly Tyr Leu Thr Leu Asn
370 375 380
Asn Gly Ser Gln Ala Val Gly Arg Ser Ser Phe Tyr Cys Leu Glu Tyr
385 390 395 400
Phe Pro Ser Gln Met Leu Arg Thr Gly Asn Asn Phe Ser Phe Ser Tyr
405 410 415
Thr Phe Glu Asp Val Pro Phe His Ser Ser Tyr Ala His Ser Gln Ser
420 425 430
Leu Asp Arg Leu Met Asn Pro Leu Ile Asp Gln Tyr Leu Tyr Tyr Leu
435 440 445
Ser Arg Thr Gln Ser Thr Gly Gly Thr Ala Gly Thr Gln Gln Leu Leu
450 455 460
Phe Ser Arg Ala Gly Pro Ser Asn Met Ser Ala Gln Ala Arg Asn Trp
465 470 475 480
Leu Pro Gly Pro Cys Tyr Arg Gln Gln Arg Val Ser Thr Thr Leu Ser
485 490 495
Gln Asn Asn Asn Ser Asn Phe Ala Trp Thr Gly Ala Thr Lys Tyr His
500 505 510
Leu Asn Gly Arg Asp Ser Leu Val Asn Pro Gly Val Ala Met Ala Thr
515 520 525
Asn Lys Asp Asp Glu Asp Arg Phe Phe Pro Ser Ser Gly Ile Leu Met
530 535 540
Phe Gly Lys Gln Gly Ala Gly Lys Asp Asn Val Asp Tyr Ser Asn Val
545 550 555 560
Met Leu Thr Ser Glu Glu Glu Ile Lys Thr Thr Asn Pro Val Ala Thr
565 570 575
Glu Gln Tyr Gly Val Val Ala Asp Asn Leu Gln Gln Gln Asn Thr Ala
580 585 590
Pro Thr Val Gly Ala Val Asn Ser Gln Gly Ala Leu Pro Gly Met Val
595 600 605
Trp Gln Asn Arg Asp Val Tyr Leu Gln Gly Pro Ile Trp Ala Lys Ile
610 615 620
Pro His Thr Asp Gly Asn Phe His Pro Ser Pro Leu Met Gly Gly Phe
625 630 635 640
Gly Leu Lys His Pro Pro Pro Gln Ile Leu Ile Lys Asn Thr Pro Val
645 650 655
Pro Ala Asp Pro Pro Thr Ala Phe Asn Gln Ala Lys Leu Asn Ser Phe
660 665 670
Ile Ala Gln Tyr Ser Thr Gly Gln Val Ser Val Glu Ile Glu Trp Glu
675 680 685
Leu Gln Lys Glu Asn Ser Lys Arg Trp Asn Pro Glu Ile Gln Tyr Thr
690 695 700
Ser Asn Tyr Tyr Lys Ser Thr Asn Ala Asp Phe Ala Val Asn Thr Glu
705 710 715 720
Gly Val Tyr Ser Glu Pro Arg Pro Ile Gly Thr Arg Tyr Leu Thr Arg
725 730 735
Asn Leu
<210>103
<211>738
<212>PRT
<213>vp1,克隆rh.49
<400>103
Met Ala Ala Asp Gly Tyr Leu Pro Asp Trp Leu Glu Asp Asn Leu Ser
1 5 10 15
Glu Gly Ile Arg Glu Trp Trp Asp Leu Lys Pro Gly Ala Pro Lys Pro
20 25 30
Lys Ala Asn Gln Gln Lys Gln Asp Asp Gly Arg Gly Leu Val Leu Pro
35 40 45
Gly Tyr Lys Tyr Leu Gly Pro Phe Asn Gly Leu Asp Lys Gly Glu Pro
50 55 60
Val Asn Ala Ala Asp Ala Ala Ala Leu Glu His Asp Lys Ala Tyr Asp
65 70 75 80
Gln Gln Leu Lys Ala Gly Asp Asn Pro His Leu Arg Tyr Asn His Ala
85 90 95
Asp Ala Glu Phe Gln Glu Arg Leu Gln Glu Asp Thr Ser Phe Gly Gly
100 105 110
Asn Leu Gly Arg Ala Val Phe Gln Ala Lys Lys Arg Val Leu Glu Pro
115 120 125
Leu Gly Leu Val Glu Glu Gly Ala Lys Thr Ala Pro Gly Lys Lys Arg
130 135 140
Pro Val Glu Pro Ser Pro Gln Arg Ser Pro Asp Ser Ser Thr Gly Ile
145 150 155 160
Gly Lys Lys Gly Gln Gln Pro Ala Arg Lys Arg Leu Asn Phe Gly Gln
165 170 175
Thr Gly Asp Ser Glu Ser Val Pro Asp Pro Gln Leu Ile Gly Glu Pro
180 185 190
Pro Ala Ala Pro Ser Ser Val Gly Ser Gly Thr Met Ala Ala Gly Gly
195 200 205
Gly Ala Pro Met Ala Asp Asn Asn Glu Gly Ala Asp Gly Val Gly Ser
210 215 220
Ser Ser Gly Asn Trp His Cys Asp Ser Thr Trp Leu Gly Asp Arg Val
225 230 235 240
Ile Thr Thr Ser Thr Arg Thr Trp Ala Leu Pro Thr Tyr Asn Asn His
245 250 255
Leu Tyr Lys Gln Ile Ser Asn Gly Thr Ser Gly Gly Ser Thr Asn Asp
260 265 270
Asn Thr Tyr Phe Gly Tyr Ser Thr Pro Trp Gly Tyr Phe Asp Phe Asn
275 280 285
Arg Phe His Cys His Phe Ser Pro Arg Asp Trp Gln Arg Leu Ile Asn
290 295 300
Asn Asn Trp Gly Phe Arg Pro Lys Arg Leu Ser Phe Lys Leu Phe Asn
305 310 315 320
Ile Gln Val Lys Glu Val Thr Gln Asn Glu Gly Thr Lys Thr Ile Ala
325 330 335
Asn Asn Leu Thr Ser Thr Ile Gln Val Phe Thr Asp Ser Glu Tyr Gln
340 345 350
Leu Pro Tyr Val Leu Gly Ser Ala His Gln Gly Cys Leu Pro Pro Phe
355 360 365
Pro Ala Asp Val Phe Met Ile Pro Gln Tyr Gly Asn Leu Thr Leu Asn
370 375 380
Asn Gly Ser Gln Ala Val Gly Arg Ser Ser Phe Tyr Cys Leu Glu Tyr
385 390 395 400
Phe Pro Ser Gln Met Leu Arg Thr Gly Asn Asn Phe Ser Phe Ser Tyr
405 410 415
Thr Phe Glu Asp Val Pro Phe His Ser Ser Tyr Ala His Ser Gln Ser
420 425 430
Leu Asp Arg Leu Met Asn Pro Leu Ile Asp Gln Tyr Leu Tyr Tyr Leu
435 440 445
Ser Arg Thr Gln Ser Thr Gly Gly Thr Ala Gly Thr Gln Gln Leu Leu
450 455 460
Phe Ser Gln Ala Gly Pro Ser Asn Met Ser Ala Gln Ala Arg Asn Trp
465 470 475 480
Leu Pro Gly Pro Cys Tyr Arg Gln Gln Arg Val Ser Thr Thr Leu Ser
485 490 495
Gln Asn Asn Asn Ser Asn Phe Ala Trp Thr Gly Ala Thr Lys Tyr His
500 505 510
Leu Asn Gly Arg Asp Ser Leu Val Asn Pro Gly Val Ala Met Ala Thr
515 520 525
Asn Lys Asp Asp Glu Asp Arg Phe Phe Pro Ser Ser Gly Ile Leu Met
530 535 540
Phe Gly Lys Gln Gly Ala Gly Lys Asp Asn Met Gly Tyr Ser Asn Val
545 550 555 560
Met Leu Thr Ser Glu Glu Glu Ile Lys Thr Thr Asn Pro Val Ala Thr
565 570 575
Glu Gln Tyr Gly Val Val Ala Asp Asn Leu Gln Gln Gln Asn Thr Ala
580 585 590
Pro Ile Val Gly Ala Val Asn Ser Gln Gly Ala Leu Pro Gly Met Val
595 600 605
Trp Gln Asn Arg Asp Val Tyr Leu Gln Gly Pro Ile Trp Ala Lys Ile
610 615 620
Pro His Thr Asp Gly Asn Phe His Pro Ser Pro Leu Met Gly Gly Phe
625 630 635 640
Gly Leu Lys His Pro Pro Pro Gln Ile Leu Ile Lys Asn Thr Pro Val
645 650 655
Pro Ala Asp Pro Pro Thr Ala Phe Asn Gln Ala Lys Leu Asn Ser Phe
660 665 670
Ile Thr Gln Tyr Gly Thr Gly Gln Val Ser Val Glu Ile Glu Trp Glu
675 680 685
Leu Gln Lys Glu Asn Ser Lys Arg Trp Asn Pro Glu Ile Gln Tyr Thr
690 695 700
Ser Asn Tyr Tyr Lys Ser Thr Asn Val Asp Phe Ala Val Asn Thr Glu
705 710 715 720
Gly Val Tyr Ser Glu Pro Arg Pro Ile Gly Thr Arg Tyr Leu Thr Arg
725 730 735
Asn Leu
<210>104
<211>738
<212>PRT
<213>vp1,克隆rh.51
<400>104
Met Val Ala Asp Gly Tyr Leu Pro Asp Trp Leu Glu Asp Asn Leu Ser
1 5 10 15
Glu Gly Ile Arg Glu Trp Trp Asp Leu Lys Pro Gly Ala Pro Lys Pro
20 25 30
Lys Ala Asn Gln Gln Lys Gln Gly Asp Gly Arg Gly Leu Val Leu Pro
35 40 45
Gly Tyr Lys Tyr Leu Gly Pro Phe Asn Gly Leu Asp Lys Gly Glu Pro
50 55 60
Val Asn Ala Ala Asp Ala Ala Ala Leu Glu His Asp Lys Ala Tyr Asp
65 70 75 80
Gln Gln Leu Lys Ala Gly Asp Asn Pro Tyr Leu Arg Tyr Asn His Ala
85 90 95
Asp Ala Glu Leu Gln Glu Arg Leu Gln Glu Asp Thr Ser Phe Gly Gly
100 105 110
Asn Leu Gly Arg Ala Val Phe Gln Ala Lys Lys Arg Val Leu Glu Pro
115 120 125
Leu Gly Leu Val Glu Glu Gly Ala Lys Thr Ala Pro Gly Lys Lys Arg
130 135 140
Pro Val Glu Pro Ser Pro Gln Arg Ser Pro Asp Ser Ser Thr Gly Ile
145 150 155 160
Gly Lys Lys Gly Gln Gln Pro Ala Arg Lys Arg Leu Asn Phe Gly Gln
165 170 175
Thr Gly Asp Ser Glu Ser Val Pro Asp Pro Gln Pro Ile Gly Glu Pro
180 185 190
Pro Ala Ala Pro Ser Ser Val Gly Ser Gly Thr Met Ala Ala Gly Gly
195 200 205
Gly Ala Pro Met Ala Asp Asn Asn Glu Gly Ala Asp Gly Val Gly Ser
210 215 220
Ser Ser Gly Asn Trp His Cys Asp Ser Thr Trp Leu Gly Asp Arg Val
225 230 235 240
Ile Thr Thr Ser Thr Arg Thr Trp Ala Leu Pro Thr Tyr Asn Asn His
245 250 255
Leu Tyr Lys Gln Ile Ser Asn Gly Thr Ser Gly Gly Ser Thr Asn Asp
260 265 270
Asn Thr Tyr Phe Gly Tyr Ser Thr Pro Trp Gly Tyr Phe Asp Phe Asn
275 280 285
Arg Phe His Cys His Phe Ser Pro Arg Asp Trp Gln Arg Leu Ile Asn
290 295 300
Asn Asn Trp Gly Phe Arg Pro Lys Arg Leu Ser Phe Lys Leu Phe Asn
305 310 315 320
Ile Gln Val Lys Glu Val Thr Gln Asn Glu Gly Thr Lys Thr Ile Ala
325 330 335
Asn Asn Leu Thr Ser Thr Ile Gln Val Phe Thr Asp Ser Glu Tyr Gln
340 345 350
Leu Pro Tyr Val Leu Gly Ser Ala His Gln Gly Cys Gln Pro Pro Phe
355 360 365
Pro Ala Asp Val Phe Met Ile Pro Gln Tyr Gly Tyr Leu Thr Leu Asn
370 375 380
Asn Gly Ser Gln Ala Val Gly Arg Ser Ser Phe Tyr Cys Leu Glu Tyr
385 390 395 400
Phe Pro Ser Gln Met Leu Arg Thr Gly Asn Asn Phe Ser Phe Ser Tyr
405 410 415
Thr Phe Glu Asp Val Pro Phe His Ser Ser Tyr Ala His Ser Gln Ser
420 425 430
Leu Asp Arg Leu Met Asn Pro Leu Ile Asp Gln Tyr Leu Tyr Tyr Leu
435 440 445
Ser Arg Thr Gln Ser Thr Gly Gly Thr Ala Gly Thr Gln Gln Leu Leu
450 455 460
Phe Ser Gln Ala Gly Pro Ser Asn Met Ser Ala Gln Ala Arg Asn Trp
465 470 475 480
Leu Pro Gly Pro Cys Tyr Arg Gln Gln Arg Val Ser Thr Thr Leu Ser
485 490 495
Gln Asn Asn Asn Ser Asn Phe Ala Trp Thr Gly Ala Thr Lys Tyr His
500 505 510
Leu Asn Gly Arg Asp Ser Leu Val Asn Pro Gly Val Ala Met Ala Thr
515 520 525
Asn Lys Asp Asp Glu Asp Arg Phe Phe Pro Ser Ser Gly Ile Leu Met
530 535 540
Phe Gly Lys Gln Gly Ala Gly Lys Asp Asn Val Asp Tyr Ser Asn Val
545 550 555 560
Met Leu Thr Ser Glu Glu Glu Ile Lys Thr Thr Asn Pro Val Ala Thr
565 570 575
Glu Gln Tyr Gly Val Val Ala Asp Asn Leu Gln Gln Gln Asn Thr Ala
580 585 590
Pro Ile Val Gly Ala Val Asn Ser Gln Gly Ala Leu Pro Gly Met Val
595 600 605
Trp Gln Asn Arg Asp Val Tyr Leu Gln Gly Pro Ile Trp Ala Lys Ile
610 615 620
Pro His Thr Asp Gly Asn Phe His Pro Ser Pro Leu Met Gly Gly Phe
625 630 635 640
Gly Leu Lys His Pro Pro Pro Gln Ile Leu Ile Lys Asn Thr Pro Val
645 650 655
Pro Ala Asp Pro Pro Thr Ala Phe Asn Gln Ala Lys Leu Asn Ser Phe
660 665 670
Ile Thr Gln Tyr Ser Thr Gly Gln Val Ser Val Glu Ile Glu Trp Glu
675 680 685
Pro Gln Lys Glu Asn Ser Lys Arg Trp Asn Pro Glu Ile Gln Tyr Thr
690 695 700
Ser Asn Tyr Tyr Lys Ser Thr Asn Val Asp Phe Ala Val Asn Thr Glu
705 710 715 720
Gly Val Tyr Ser Glu Pro Arg Pro Ile Gly Thr Arg Tyr Leu Thr Arg
725 730 735
Asn Leu
<210>105
<211>738
<212>PRT
<213>vp1,克隆rh.57
<400>105
Met Ala Ala Asp Gly Tyr Leu Pro Asp Trp Leu Glu Asp Asn Leu Ser
1 5 10 15
Glu Gly Ile Arg Glu Trp Trp Ala Leu Lys Pro Gly Ala Pro Lys Pro
20 25 30
Lys Ala Asn Gln Gln Lys Gln Asp Asp Gly Arg Gly Leu Val Leu Pro
35 40 45
Gly Tyr Lys Tyr Leu Gly Pro Phe Asn Gly Leu Asp Lys Gly Glu Pro
50 55 60
Val Asn Ala Ala Asp Ala Ala Ala Leu Glu His Asp Lys Ala Tyr Asp
65 70 75 80
Gln Gln Leu Gln Ala Gly Asp Asn Pro Tyr Leu Arg Tyr Asn His Ala
85 90 95
Asp Ala Glu Phe Gln Glu Arg Leu Gln Glu Asp Thr Ser Phe Gly Gly
100 105 110
Asn Leu Gly Arg Ala Val Phe Gln Ala Lys Lys Arg Val Leu Glu Pro
115 120 125
Leu Gly Leu Val Glu Glu Gly Ala Lys Thr Ala Pro Gly Lys Lys Arg
130 135 140
Pro Val Glu Pro Ser Pro Gln Arg Ser Pro Asp Ser Ser Thr Gly Ile
145 150 155 160
Gly Lys Lys Gly Gln Gln Pro Ala Arg Lys Arg Leu Asn Phe Gly Gln
165 170 175
Thr Gly Asp Ser Glu Ser Val Pro Asp Pro Gln Pro Ile Gly Glu Pro
180 185 190
Pro Ala Ala Pro Ser Ser Val Gly Ser Gly Thr Met Ala Ala Gly Gly
195 200 205
Gly Ala Pro Met Ala Asp Asn Asn Glu Gly Ala Asp Gly Val Gly Ser
210 215 220
Ser Ser Gly Asn Trp His Cys Asp Ser Thr Trp Leu Gly Asp Arg Val
225 230 235 240
Ile Thr Thr Ser Thr Arg Thr Trp Ala Leu Pro Thr Tyr Asn Asn His
245 250 255
Leu Tyr Lys Gln Thr Ser Asn Gly Thr Ser Gly Gly Ser Thr Asn Asp
260 265 270
Asn Thr Tyr Phe Gly Tyr Ser Thr Pro Trp Gly Tyr Phe Asp Phe Asn
275 280 285
Arg Phe His Cys His Phe Ser Pro Arg Asp Trp Gln Arg Leu Ile Asn
290 295 300
Asn Asn Trp Gly Phe Arg Pro Lys Arg Leu Ser Phe Lys Leu Phe Asn
305 310 315 320
Ile Gln Val Lys Glu Val Thr Gln Asn Glu Gly Thr Lys Thr Ile Ala
325 330 335
Asn Asn Leu Thr Ser Thr Ile Gln Val Phe Thr Asp Ser Glu Tyr Gln
340 345 350
Leu Pro Tyr Val Leu Gly Ser Ala His Gln Gly Cys Leu Pro Pro Phe
355 360 365
Pro Ala Asp Val Phe Met Ile Pro Gln Tyr Gly Tyr Leu Thr Leu Asn
370 375 380
Asn Gly Ser Gln Ala Val Gly Arg Ser Ser Phe Tyr Cys Leu Glu Tyr
385 390 395 400
Phe Pro Ser Gln Met Leu Arg Thr Gly Asn Asn Phe Ser Phe Ser Tyr
405 410 415
Thr Phe Glu Asp Val Pro Phe His Ser Ser Tyr Ala His Ser Gln Ser
420 425 430
Leu Asp Arg Leu Met Asn Pro Leu Ile Asp Gln Tyr Leu Tyr Tyr Leu
435 440 445
Ser Arg Thr Gln Ser Thr Gly Gly Thr Ala Gly Thr Gln Gln Leu Leu
450 455 460
Phe Ser Gln Ala Gly Pro Ser Asn Met Ser Ala Gln Ala Arg Asn Trp
465 470 475 480
Leu Pro Gly Pro Cys Tyr Arg Gln Gln Arg Val Ser Thr Thr Leu Ser
485 490 495
Gln Asn Asn Asn Ser Asn Phe Ala Trp Thr Gly Ala Thr Lys Tyr His
500 505 510
Leu Asn Gly Arg Asp Ser Leu Val Asn Pro Gly Val Ala Met Ala Thr
515 520 525
Asn Lys Asp Asp Glu Asp Arg Phe Phe Pro Ser Ser Gly Ile Leu Met
530 535 540
Phe Gly Lys Gln Gly Ala Gly Lys Asp Asn Val Asp Tyr Ser Asn Val
545 550 555 560
Met Leu Thr Ser Glu Glu Glu Ile Lys Thr Thr Asn Pro Val Ala Thr
565 570 575
Glu Gln Tyr Gly Val Val Ala Asp Asn Leu Gln Gln Gln Asn Thr Ala
580 585 590
Pro Ile Val Gly Ala Val Asn Ser Gln Gly Ala Leu Pro Gly Met Val
595 600 605
Trp Gln Asn Arg Asp Val Tyr Leu Gln Gly Pro Ile Trp Ala Lys Ile
610 615 620
Pro His Thr Asp Gly Asn Phe His Pro Ser Pro Leu Met Gly Gly Phe
625 630 635 640
Gly Leu Lys His Pro Pro Pro Gln Ile Leu Ile Lys Asn Thr Pro Val
645 650 655
Pro Ala Asp Pro Pro Thr Ala Phe Asn Gln Ala Lys Leu Asn Ser Phe
660 665 670
Ile Thr Gln Tyr Ser Thr Gly Gln Val Ser Ala Glu Ile Glu Trp Glu
675 680 685
Leu Gln Lys Glu Asn Ser Lys Arg Trp Asn Pro Glu Ile Gln Tyr Thr
690 695 700
Ser Asn Tyr Tyr Lys Ser Thr Asn Val Asp Phe Ala Val Asn Thr Glu
705 710 715 720
Gly Val Tyr Ser Glu Pro Arg Pro Ile Gly Thr Arg Tyr Leu Thr Arg
725 730 735
Asn Leu
<210>106
<211>738
<212>PRT
<213>vp1,克隆rh.58
<400>106
Met Ala Ala Asp Gly Tyr Leu Pro Asp Trp Leu Glu Asp Asn Leu Ser
1 5 10 15
Glu Gly Ile Arg Glu Trp Trp Asp Leu Lys Pro Gly Ala Pro Lys Pro
20 25 30
Lys Ala Asn Gln Gln Lys Gln Asp Asp Gly Arg Gly Leu Val Leu Pro
35 40 45
Gly Tyr Lys Tyr Leu Gly Pro Phe Asn Gly Leu Asp Lys Gly Glu Pro
50 55 60
Val Asn Ala Ala Asp Ala Ala Ala Leu Glu His Asp Lys Ala Tyr Asp
65 70 75 80
Gln Gln Leu Lys Ala Gly Asp Asn Pro Tyr Leu Arg Tyr Asn His Ala
85 90 95
Asp Ala Glu Phe Gln Glu Arg Leu Gln Glu Asp Thr Ser Phe Gly Gly
100 105 110
Asn Leu Gly Arg Ala Val Phe Gln Ala Lys Lys Arg Val Leu Glu Pro
115 120 125
Leu Gly Leu Val Glu Glu Ala Ala Lys Thr Ala Pro Gly Lys Lys Arg
130 135 140
Pro Val Glu Pro Ser Pro Gln Arg Ser Pro Asp Ser Ser Thr Gly Ile
145 150 155 160
Gly Lys Lys Gly Gln Gln Pro Ala Arg Lys Arg Leu Asn Phe Gly Gln
165 170 175
Thr Gly Asp Ser Glu Ser Val Pro Asp Pro Gln Pro Ile Gly Glu Pro
180 185 190
Pro Ala Ala Pro Ser Ser Val Gly Ser Gly Thr Met Ala Ala Gly Gly
195 200 205
Gly Ala Pro Met Ala Asp Asn Asn Glu Gly Ala Asp Gly Val Gly Ser
210 215 220
Ser Ser Gly Asn Trp His Cys Asp Ser Thr Trp Leu Gly Asp Arg Val
225 230 235 240
Ile Thr Thr Ser Thr Arg Thr Trp Ala Leu Pro Thr Tyr Asn Asn His
245 250 255
Leu Tyr Lys Gln Ile Ser Asn Gly Thr Ser Gly Gly Ser Thr Asn Asp
260 265 270
Asn Thr Tyr Phe Gly Tyr Ser Thr Pro Trp Gly Tyr Phe Asp Phe Asn
275 280 285
Arg Phe His Cys His Phe Ser Pro Arg Asp Trp Gln Arg Leu Ile Asn
290 295 300
Asn Asn Trp Gly Phe Arg Pro Lys Arg Leu Ser Phe Lys Leu Phe Asn
305 310 315 320
Ile Gln Val Lys Glu Val Thr Gln Asn Glu Gly Thr Lys Thr Ile Ala
325 330 335
Asn Asn Leu Thr Ser Thr Ile Gln Val Phe Thr Asp Ser Glu Tyr Gln
340 345 350
Leu Pro Tyr Val Leu Gly Ser Ala His Gln Gly Cys Leu Pro Pro Phe
355 360 365
Pro Ala Asp Val Phe Met Ile Pro Gln Tyr Gly Tyr Leu Thr Leu Asn
370 375 380
Asn Gly Ser Gln Ala Val Gly Arg Ser Ser Phe Tyr Cys Leu Glu Tyr
385 390 395 400
Phe Pro Ser Gln Met Leu Arg Thr Gly Asn Asn Phe Ser Phe Ser Tyr
405 410 415
Thr Phe Glu Asp Val Pro Phe His Ser Ser Tyr Ala His Ser Gln Ser
420 425 430
Leu Asp Arg Leu Met Asn Pro Leu Ile Asp Gln Tyr Leu Tyr Tyr Leu
435 440 445
Ser Arg Thr Gln Ser Thr Gly Gly Thr Ala Gly Thr Gln Gln Leu Leu
450 455 460
Phe Ser Gln Ala Gly Pro Ser Asn Met Ser Ala Gln Ala Arg Asn Trp
465 470 475 480
Leu Pro Gly Pro Cys Tyr Arg Gln Gln Arg Val Ser Thr Thr Leu Ser
485 490 495
Gln Asn Asn Asn Ser Asn Phe Ala Trp Thr Gly Ala Thr Lys Tyr His
500 505 510
Leu Asn Gly Arg Asp Ser Leu Val Asn Pro Gly Val Ala Met Ala Thr
515 520 525
Asn Lys Asp Asp Glu Asp Arg Phe Phe Pro Ser Ser Gly Ile Leu Met
530 535 540
Phe Gly Lys Gln Gly Ala Gly Lys Asp Asn Val Asp Tyr Ser Asn Val
545 550 555 560
Met Leu Thr Ser Glu Glu Glu Ile Lys Thr Thr Asn Pro Val Ala Thr
565 570 575
Glu Gln Tyr Gly Val Val Ala Asp Asn Leu Gln Gln Gln Asn Thr Ala
580 585 590
Pro Ile Val Gly Ala Val Asn Ser Gln Gly Ala Leu Pro Gly Met Val
595 600 605
Trp Gln Asn Arg Asp Val Tyr Leu Gln Gly Pro Ile Trp Ala Lys Ile
610 615 620
Pro His Thr Asp Gly Asn Phe His Pro Ser Pro Leu Met Gly Gly Phe
625 630 635 640
Gly Leu Lys His Pro Pro Pro Gln Ile Leu Ile Lys Ser Thr Pro Val
645 650 655
Pro Ala Asp Pro Pro Thr Ala Phe Asn Gln Ala Lys Leu Asn Ser Phe
660 665 670
Ile Thr Gln Tyr Ser Thr Gly Gln Val Ser Val Glu Ile Glu Trp Glu
675 680 685
Leu Gln Lys Glu Asn Ser Lys Cys Trp Asn Pro Glu Ile Gln Tyr Thr
690 695 700
Ser Asn Tyr Tyr Lys Ser Thr Asn Val Asp Phe Ala Val Asn Thr Glu
705 710 715 720
Gly Val Tyr Ser Glu Pro Arg Pro Ile Gly Thr Arg Tyr Leu Thr Arg
725 730 735
Asn Leu
<210>107
<211>738
<212>PRT
<213>vp1,克隆rh.61
<400>107
Met Ala Ala Asp Gly Tyr Leu Pro Asp Trp Leu Glu Asp Asn Leu Ser
1 5 10 15
Glu Gly Ile Arg Glu Trp Trp Asp Leu Lys Pro Gly Ala Pro Lys Pro
20 25 30
Lys Ala Asn Gln Gln Lys Gln Asp Asp Gly Arg Gly Leu Val Leu Pro
35 40 45
Gly Tyr Lys Tyr Leu Gly Pro Phe Asn Gly Leu Asp Lys Gly Glu Pro
50 55 60
Val Asn Ala Ala Asp Ala Ala Ala Leu Glu His Asp Lys Ala Tyr Asp
65 70 75 80
Gln Gln Leu Lys Ala Gly Asp Asn Pro Tyr Leu Arg Tyr Asn His Ala
85 90 95
Asp Ala Glu Phe Gln Glu Arg Leu Gln Glu Asp Thr Ser Phe Gly Gly
100 105 110
Asn Leu Gly Arg Ala Val Phe Gln Ala Lys Lys Arg Val Leu Glu Pro
115 120 125
Leu Gly Leu Val Glu Glu Gly Ala Lys Thr Ala Pro Gly Lys Lys Arg
130 135 140
Pro Val Glu Pro Ser Pro Gln Arg Ser Pro Asp Ser Ser Thr Gly Ile
145 150 155 160
Gly Lys Lys Gly Gln Gln Pro Ala Arg Lys Arg Leu Asn Phe Gly Gln
165 170 175
Thr Gly Asp Ser Glu Ser Val Pro Asp Pro Gln Pro Ile Gly Glu Pro
180 185 190
Pro Ala Ala Pro Ser Ser Val Gly Ser Gly Thr Met Ala Ala Gly Gly
195 200 205
Gly Ala Pro Met Ala Asp Asn Asn Glu Gly Ala Asp Gly Val Gly Ser
210 215 220
Ser Ser Gly Asn Trp His Cys Asp Ser Thr Trp Leu Gly Asp Arg Val
225 230 235 240
Ile Thr Thr Ser Thr Arg Thr Trp Ala Leu Pro Thr Tyr Asn Asn His
245 250 255
Leu Tyr Lys Gln Ile Ser Asn Gly Thr Ser Gly Gly Ser Thr Asn Asp
260 265 270
Asn Thr Tyr Phe Gly Tyr Ser Thr Pro Trp Gly Tyr Phe Asp Phe Asn
275 280 285
Arg Phe His Cys His Phe Ser Pro Arg Asp Trp Gln Arg Pro Ile Asn
290 295 300
Asn Asn Trp Gly Phe Arg Pro Lys Arg Leu Ser Phe Lys Leu Phe Asn
305 310 315 320
Ile Gln Val Lys Glu Val Thr Gln Asn Glu Gly Thr Lys Thr Ile Ala
325 330 335
Asn Asn Leu Thr Ser Thr Ile Gln Val Phe Thr Asp Ser Glu Tyr Gln
340 345 350
Leu Pro Tyr Val Leu Gly Ser Ala His Gln Gly Cys Leu Pro Pro Phe
355 360 365
Pro Ala Asp Val Phe Met Ile Pro Gln Tyr Gly Tyr Leu Thr Leu Asn
370 375 380
Asn Gly Ser Gln Ala Val Gly Arg Ser Ser Phe Tyr Cys Leu Glu Tyr
385 390 395 400
Phe Pro Ser Gln Met Leu Arg Thr Gly Asn Asn Phe Ser Phe Ser Tyr
405 410 415
Pro Phe Glu Asp Val Pro Phe His Ser Ser Tyr Ala His Ser Gln Ser
420 425 430
Leu Asp Arg Leu Met Asn Pro Leu Ile Asp Gln Tyr Leu Tyr Tyr Leu
435 440 445
Ser Arg Thr Gln Ser Thr Gly Gly Thr Ala Gly Thr Gln Gln Leu Leu
450 455 460
Phe Ser Gln Ala Gly Pro Ser Asn Met Ser Ala Gln Ala Arg Asn Trp
465 470 475 480
Leu Pro Gly Pro Cys Tyr Arg Gln Gln Arg Val Ser Thr Thr Leu Ser
485 490 495
Gln Asn Asn Asn Ser Asn Phe Ala Trp Thr Gly Ala Thr Lys Tyr His
500 505 510
Leu Asn Gly Arg Asp Ser Leu Val Asn Pro Gly Val Ala Met Ala Thr
515 520 525
Asn Lys Asp Asp Glu Asp Arg Phe Phe Pro Ser Ser Gly Ile Leu Met
530 535 540
Phe Gly Lys Gln Gly Ala Gly Lys Asp Asn Val Asp Tyr Ser Asn Val
545 550 555 560
Met Leu Thr Ser Glu Glu Glu Ile Lys Thr Thr Asn Pro Val Ala Thr
565 570 575
Glu Gln Tyr Gly Val Val Ala Asp Asn Leu Gln Gln Gln Asp Thr Ala
580 585 590
Pro Ile Val Gly Ala Val Asn Ser Gln Gly Ala Leu Pro Gly Met Val
595 600 605
Trp Gln Asn Arg Asp Val Tyr Leu Gln Gly Pro Ile Trp Ala Lys Ile
610 615 620
Pro His Thr Asp Gly Asn Phe His Pro Ser Pro Leu Met Gly Gly Phe
625 630 635 640
Gly Leu Lys His Pro Pro Pro Gln Val Leu Ile Lys Asn Thr Pro Val
645 650 655
Pro Ala Asp Pro Pro Thr Ala Phe Asn Gln Ala Lys Leu Asn Ser Phe
660 665 670
Ile Thr Gln Tyr Ser Thr Gly Gln Val Ser Val Glu Ile Glu Trp Glu
675 680 685
Leu Gln Lys Glu Asn Ser Lys Arg Trp Asn Pro Glu Ile Gln Tyr Thr
690 695 700
Ser Asn Tyr Tyr Lys Ser Thr Asn Val Asp Phe Ala Val Asn Thr Glu
705 710 715 720
Gly Val Tyr Ser Glu Pro Arg Pro Ile Gly Thr Arg Tyr Leu Thr Arg
725 730 735
Asn Leu
<210>108
<211>738
<212>PRT
<213>vp1,克隆rh.50
<400>108
Met Ala Ala Asp Gly Tyr Leu Pro Asp Trp Leu Glu Asp Asn Leu Ser
1 5 10 15
Glu Gly Ile Arg Glu Trp Trp Asp Leu Lys Pro Gly Ala Pro Lys Pro
20 25 30
Lys Ala Asn Gln Gln Lys Gln Asp Asp Gly Arg Gly Leu Val Leu Pro
35 40 45
Gly Tyr Lys Tyr Leu Gly Pro Phe Asn Gly Leu Asp Lys Gly Glu Pro
50 55 60
Val Asn Ala Ala Asp Ala Ala Ala Leu Glu His Asp Lys Ala Tyr Asp
65 70 75 80
Gln Gln Leu Lys Ala Gly Asp Asn Pro Tyr Leu Arg Tyr Asn His Ala
85 90 95
Asp Ala Glu Phe Gln Glu Arg Leu Gln Glu Asp Thr Ser Phe Gly Gly
100 105 110
Asn Leu Gly Arg Ala Val Phe Gln Ala Lys Lys Arg Val Leu Glu Pro
115 120 125
Leu Gly Leu Val Glu Glu Gly Ala Lys Thr Ala Pro Gly Lys Lys Arg
130 135 140
Pro Val Glu Pro Ser Pro Gln Arg Ser Pro Asp Ser Ser Thr Gly Ile
145 150 155 160
Gly Lys Lys Gly Gln Gln Pro Ala Gly Lys Arg Leu Asn Phe Gly Gln
165 170 175
Thr Gly Asp Ser Glu Ser Val Pro Asp Pro Gln Pro Ile Gly Glu Pro
180 185 190
Pro Ala Ala Pro Ser Ser Val Gly Ser Gly Thr Met Ala Ala Gly Gly
195 200 205
Gly Ala Pro Met Ala Asp Asn Asn Glu Gly Ala Asp Gly Val Gly Ser
210 215 220
Ser Ser Gly Asn Trp His Cys Asp Ser Thr Trp Leu Gly Asp Arg Val
225 230 235 240
Ile Thr Thr Ser Thr Arg Thr Trp Ala Leu Pro Thr Tyr Asn Asn His
245 250 255
Leu Tyr Lys Gln Ile Ser Asn Gly Thr Ser Gly Gly Ser Thr Asn Asp
260 265 270
Asn Thr Tyr Phe Gly Tyr Ser Thr Pro Trp Gly Tyr Phe Asp Phe Asn
275 280 285
Arg Phe His Cys His Phe Ser Pro Arg Asp Trp Gln Arg Leu Ile Asn
290 295 300
Asn Asn Trp Gly Phe Arg Pro Lys Arg Leu Ser Phe Lys Leu Phe Asn
305 310 315 320
Ile Gln Val Lys Glu Val Thr Gln Asn Glu Gly Thr Lys Thr Ile Ala
325 330 335
Asn Asn Leu Thr Ser Thr Ile Gln Val Phe Thr Asp Ser Glu Tyr Gln
340 345 350
Leu Pro Tyr Val Leu Gly Ser Ala His Gln Gly Cys Leu Pro Pro Phe
355 360 365
Pro Ala Asp Val Phe Met Ile Pro Gln Tyr Gly Tyr Leu Thr Leu Asn
370 375 380
Asn Gly Ser Gln Ala Val Gly Arg Ser Ser Phe Tyr Cys Leu Glu Tyr
385 390 395 400
Phe Pro Ser Gln Met Leu Arg Thr Gly Asn Asn Phe Ser Phe Ser Tyr
405 410 415
Thr Phe Glu Asp Val Pro Phe His Ser Ser Tyr Ala His Ser Gln Ser
420 425 430
Leu Asp Arg Leu Met Asn Pro Leu Val Asp Gln Tyr Leu Tyr Tyr Leu
435 440 445
Ser Arg Thr Gln Ser Thr Gly Gly Thr Ala Gly Thr Gln Gln Leu Leu
450 455 460
Phe Ser Gln Ala Gly Pro Ser Asn Met Ser Ala Gln Ala Arg Asn Trp
465 470 475 480
Leu Pro Gly Pro Cys Tyr Arg Gln Gln Arg Val Ser Thr Thr Leu Ser
485 490 495
Gln Asn Asn Asn Ser Asn Phe Ala Trp Thr Gly Ala Thr Lys Tyr His
500 505 510
Leu Asn Gly Arg Asp Ser Leu Val Asn Pro Gly Val Ala Met Ala Thr
515 520 525
Asn Lys Asp Asp Glu Asp Arg Phe Phe Pro Ser Ser Gly Ile Leu Met
530 535 540
Phe Gly Lys Gln Gly Ala Gly Lys Asp Asn Val Asp Tyr Ser Asn Val
545 550 555 560
Met Leu Thr Ser Glu Glu Glu Ile Lys Thr Thr Asn Pro Val Ala Thr
565 570 575
Glu Gln Tyr Gly Val Val Ala Asp Asn Leu Gln Gln Gln Asn Thr Ala
580 585 590
Pro Ile Val Gly Ala Val Asn Ser Gln Gly Ala Leu Pro Gly Met Val
595 600 605
Trp Gln Asn Arg Asp Val Tyr Leu Gln Gly Pro Ile Trp Ala Lys Ile
610 615 620
Pro His Thr Asp Gly Asn Phe His Pro Ser Pro Leu Met Gly Gly Phe
625 630 635 640
Gly Leu Lys His Pro Pro Pro Gln Ile Leu Ile Lys Asn Thr Pro Val
645 650 655
Pro Ala Asp Pro Pro Thr Ala Phe Asn Gln Ala Lys Leu Asn Ser Phe
660 665 670
Ile Thr Gln Tyr Ser Thr Gly Gln Val Ser Val Glu Ile Glu Trp Glu
675 680 685
Leu Gln Lys Glu Asn Ser Lys Arg Trp Ser Pro Glu Ile Gln Tyr Thr
690 695 700
Ser Asn Tyr Tyr Lys Ser Thr Asn Val Asp Phe Ala Val Asn Thr Glu
705 710 715 720
Gly Val Tyr Ser Glu Pro Arg Pro Ile Gly Thr Arg Tyr Leu Thr Arg
725 730 735
Asn Leu
<210>109
<211>737
<212>PRT
<213>vp1,克隆rh.45
<400>109
Met Ala Ala Asp Gly Tyr Leu Pro Asp Trp Leu Glu Asp Asn Leu Ser
1 5 10 15
Glu Gly Ile Arg Glu Trp Trp Asp Leu Lys Pro Gly Ala Pro Lys Pro
20 25 30
Lys Ala Asn Gln Gln Lys Gln Asp Asp Gly Arg Gly Leu Val Leu Pro
35 40 45
Gly Tyr Lys Tyr Leu Gly Pro Phe Asn Gly Leu Asp Lys Gly Glu Pro
50 55 60
Val Asn Ala Ala Asp Ala Ala Ala Leu Glu His Asp Lys Ala Tyr Asp
65 70 75 80
Gln Gln Leu Lys Ala Gly Asp Asn Pro Tyr Leu Arg Tyr Asn His Ala
85 90 95
Asp Ala Glu Phe Gln Glu Arg Leu Gln Glu Asp Thr Ser Phe Gly Gly
100 105 110
Asn Leu Gly Arg Ala Val Phe Gln Ala Lys Lys Arg Val Leu Glu Pro
115 120 125
Leu Gly Leu Val Glu Glu Ala Ala Lys Thr Ala Pro Gly Lys Lys Arg
130 135 140
Pro Val Glu Pro Ser Pro Gln Arg Ser Pro Asp Ser Ser Thr Gly Ile
145 150 155 160
Gly Lys Lys Gly Gln Gln Pro Ala Arg Lys Arg Leu Asn Phe Gly Gln
165 170 175
Thr Gly Asp Ser Glu Ser Val Pro Asp Pro Gln Pro Ile Gly Glu Pro
180 185 190
Pro Ala Gly Pro Ser Gly Leu Gly Ser Gly Thr Met Ala Ala Gly Gly
195 200 205
Gly Ala Pro Met Ala Asp Asn Asn Glu Gly Ala Asp Gly Val Gly Asn
210 215 220
Ala Ser Gly Asn Trp His Cys Asp Ser Thr Trp Leu Gly Asp Arg Val
225 230 235 240
Ile Thr Thr Ser Thr Arg Thr Trp Ala Leu Pro Thr Tyr Asn Asn His
245 250 255
Leu Tyr Lys Gln IIe Ser Ser Gln Ser Ala Gly Ser Thr Asn Asp Asn
260 265 270
Val Tyr Phe Gly Tyr Ser Thr Pro Trp Gly Tyr Phe Asp Phe Asn Arg
275 280 285
Phe His Cys His Phe Ser Pro Arg Asp Trp Gln Arg Leu Ile Asn Asn
290 295 300
Asn Trp Gly Phe Arg Pro Lys Lys Leu Asn Phe Lys Leu Phe Asn Ile
305 310 315 320
Gln Val Lys Glu Val Thr Thr Asn Asp Gly Val Thr Thr Ile Ala Asn
325 330 335
Asn Leu Thr Ser Thr Val Gln Val Phe Ser Asp Ser Glu Tyr Gln Leu
340 345 350
Pro Tyr Val Leu Gly Ser Ala His Gln Gly Cys Leu Pro Pro Phe Pro
355 360 365
Ala Asp Val Phe Met Ile Pro Gln Tyr Gly Tyr Leu Thr Leu Asn Asn
370 375 380
Gly Ser Gln Ala Val Gly Arg Ser Ser Phe Tyr Cys Leu Glu Tyr Phe
385 390 395 400
Pro Ser Gln Met Leu Arg Thr Gly Asn Asn Phe Ser Phe Ser Tyr Thr
405 410 415
Phe Glu Asp Val Pro Phe His Ser Ser Tyr Ala His Ser Gln Ser Leu
420 425 430
Asp Arg Leu Met Asn Pro Leu Ile Asp Gln Tyr Leu Tyr Tyr Leu Ser
435 440 445
Arg Thr Gln Ser Thr Gly Gly Thr Ala Gly Thr Gln Gln Leu Leu Phe
450 455 460
Ser Gln Ala Gly Pro Ser Asn Met Ser Thr Gln Ala Arg Asn Trp Leu
465 470 475 480
Pro Gly Pro Cys Tyr Arg Gln Gln Arg Val Ser Thr Thr Leu Ser Gln
485 490 495
Asn Asn Asn Ser Asn Phe Ala Trp Thr Gly Ala Thr Lys Tyr His Leu
500 505 510
Asn Gly Arg Asp Ser Leu Val Asn Pro Gly Val Ala Met Ala Thr Asn
515 520 525
Lys Asp Asp Glu Asp Arg Phe Phe Pro Ser Ser Gly Ile Leu Met Phe
530 535 540
Gly Lys Gln Gly Ala Gly Lys Asp Asn Val Asp Tyr Ser Asn Val Met
545 550 555 560
Leu Thr Ser Glu Glu Glu Ile Lys Thr Thr Asn Pro Val Ala Thr Glu
565 570 575
Gln Tyr Gly Val Val Ala Asp Asn Leu Gln Gln Gln Asn Thr Ala Pro
580 585 590
Ile Val Gly Ala Val Asn Ser Gln Gly Ala Leu Pro Gly Met Val Trp
595 300 605
Gln Asn Arg Asp Val Tyr Leu Gln Gly Pro Ile Trp Ala Lys Ile Pro
610 3615 620
His Thr Asp Gly Asn Phe His Pro Ser Pro Leu Met Gly Gly Phe Gly
625 630 635 640
Leu Lys His Pro Pro Pro Gln Ile Leu Ile Lys Asn Thr Pro Val Pro
645 650 655
Ala Asp Pro Pro Thr Ala Phe Asn Gln Ala Lys Leu Asn Ser Phe Ile
660 665 670
Thr Gln Tyr Ser Thr Gly Gln Val Ser Val Glu Ile Glu Trp Glu Leu
675 680 685
Gln Lys Glu Asn Ser Lys Arg Trp Asn Pro Glu Ile Gln Tyr Thr Ser
690 695 700
Asn Tyr Tyr Lys Ser Thr Asn Mal Asp Phe Ala Val Asn Thr Glu Gly
705 710 715 720
Ala Tyr Ser Glu Pro Arg Pro Ile Gly Thr Arg Tyr Leu Thr Arg Asn
725 730 735
Leu
<210>110
<211>738
<212>PRT
<213>vp1,克隆rh.59
<400>110
Met Ala Ala Asp Gly Tyr Leu Pro Asp Trp Leu Glu Asp Asn Leu Ser
1 5 10 15
Glu Gly Ile Arg Glu Trp Trp Asp Leu Lys Pro Gly Ala Pro Lys Pro
20 25 30
Lys Ala Asn Gln Gln Lys Gln Asp Asp Gly Arg Gly Leu Val Leu Pro
35 40 45
Gly Tyr Lys Tyr Leu Gly Pro Phe Asn Gly Leu Asp Lys Gly Glu Pro
50 55 60
Val Asn Ala Ala Asp Ala Ala Ala Leu Glu His Asp Lys Ala Tyr Asp
65 70 75 80
Gln Gln Leu Lys Ala Gly Asp Asn Pro Tyr Leu Arg Tyr Asn His Ala
85 90 95
Asp Ala Glu Phe Gln Glu Arg Leu Gln Glu Asp Thr Ser Phe Gly Gly
100 105 110
Asn Leu Gly Arg Ala Val Phe Gln Ala Lys Lys Arg Val Leu Glu Pro
115 120 125
Leu Gly Leu Mal Glu Glu Ala Ala Lys Thr Ala Pro Gly Lys Lys Arg
130 135 140
Pro Val Glu Pro Ser Pro Gln Arg Ser Pro Asp Ser Ser Thr Gly Ile
145 150 155 160
Gly Lys Lys Gly Gln Gln Pro Ala Arg Lys Arg Leu Asn Phe Gly Gln
165 170 175
Thr Gly Asp Ser Glu Ser Val Pro Asp Pro Gln Pro Ile Gly Glu Pro
180 185 190
Pro Ala Gly Pro Ser Gly Leu Gly Ser Gly Thr Met Ala Ala Gly Gly
195 200 205
Gly Ala Pro Met Ala Asp Asn Asn Glu Gly Ala Asp Gly Val Gly Asn
210 215 220
Ala Ser Gly Asn Trp His Cys Asp Ser Thr Trp Leu Gly Asp Arg Val
225 230 235 240
Ile Thr Thr Ser Thr Arg Thr Trp Ala Leu Pro Thr Tyr Asn Asn His
245 250 255
Leu Tyr Lys Gln Ile Ser Ser Gln Ser Ala Gly Ser Thr Asn Asp Asn
260 265 270
Val Tyr Phe Gly Tyr Ser Thr Pro Trp Gly Tyr Phe Asp Phe Asn Arg
275 280 285
Phe His Cys His Phe Ser Pro Arg Asp Trp Gln Arg Leu Ile Asn Asn
290 295 300
Asn Trp Gly Phe Arg Pro Lys Lys Leu Asn Phe Lys Leu Phe Asn Ile
305 310 315 320
Gln Val Lys Glu Val Thr Thr Asn Asp Gly Val Thr Thr Ile Ala Asn
325 330 335
Asn Pro Thr Ser Thr Val Gln Val Phe Ser Asp Ser Glu Tyr Gln Leu
340 345 350
Pro Tyr Val Leu Gly Ser Ala His Gln Gly Cys Leu Pro Pro Phe Pro
355 360 365
Ala Asp Val Phe Met Ile Pro Gln Tyr Gly Tyr Leu Thr Leu Asn Asn
370 375 380
Gly Ser Gln Ser Val Gly Arg Ser Ser Phe Tyr Cys Leu Glu Tyr Phe
385 390 395 400
Pro Ser Gln Met Leu Arg Thr Gly Asn Asn Phe Thr Phe Ser Tyr Thr
405 410 415
Phe Glu Asp Val Pro Phe His Ser Ser Tyr Ala His Ser Gln Ser Leu
420 425 430
Asp Arg Leu Met Asn Pro Leu Ile Asp Gln Tyr Leu Tyr Tyr Leu Ala
435 440 445
Arg Thr Gln Ser Asn Ala Gly Gly Thr Ala Gly Asn Arg Glu Leu Gln
450 455 460
Phe Tyr Gln Gly Gly Pro Thr Thr Met Ala Glu Gln Ala Lys Asn Trp
465 470 475 480
Leu Pro Gly Pro Cys Tyr Arg Gln Gln Arg Val Ser Thr Thr Leu Ser
485 490 495
Gln Asn Asn Asn Ser Asn Phe Ala Trp Thr Gly Ala Thr Lys Tyr His
500 505 510
Leu Asn Gly Arg Asp Ser Leu Val Asn Pro Gly Val Ala Met Ala Thr
515 520 525
Asn Lys Asp Asp Glu Asp Arg Phe Phe Pro Ser Ser Gly Ile Leu Met
530 535 540
Phe Gly Lys Gln Gly Ala Gly Lys Asp Asn Val Asp Tyr Ser Asn Val
545 550 555 560
Met Leu Thr Ser Glu Glu Glu Ile Lys Thr Thr Asn Pro Val Ala Thr
565 570 575
Glu Gln Tyr Gly Val Val Ala Asp Asn Leu Gln Gln Gln Asn Thr Ala
580 585 590
Pro Ile Val Gly Ala Val Asn Ser Gln Gly Ala Leu Pro Gly Met Val
595 600 605
Trp Gln Asn Arg Asp Val Tyr Leu Gln Gly Pro Ile Trp Ala Lys Ile
610 615 620
Pro His Thr Asp Gly Asn Phe His Pro Ser Pro Leu Met Gly Gly Phe
625 630 635 640
Gly Leu Lys His Pro Pro Pro Gln Ile Leu Ile Lys Asn Thr Pro Val
645 650 655
Pro Ala Asp Pro Pro Thr Ala Phe Asn Gln Ala Lys Leu Asn Ser Phe
660 665 670
Ile Thr Gln Tyr Ser Thr Gly Gln Val Ser Val Glu Ile Glu Trp Glu
675 680 685
Leu Gln Lys Glu Asn Ser Lys Arg Trp Asn Pro Glu Ile Gln Tyr Thr
690 695 700
Ser Asn Tyr Tyr Lys Ser Thr Asn Val Asp Phe Ala Val Asn Thr Glu
705 710 715 720
Gly Val Tyr Ser Glu Pro Arg Pro Ile Gly Thr Arg Tyr Leu Thr Arg
725 730 735
Asn Leu
<210>111
<21l>737
<212>PRT
<213>vp1,克隆rh.44
<400>111
Met Ala Ala Asp Gly Tyr Leu Pro Asp Trp Leu Glu Asp Asn Leu Ser
1 5 10 15
Glu Gly Ile Arg Glu Trp Trp Asp Leu Lys Pro Gly Ala Pro Lys Pro
20 25 30
Lys Ala Asn Gln Gln Lys Gln Asp Asp Gly Arg Gly Leu Val Leu Pro
35 40 45
Gly Tyr Lys Tyr Leu Gly Pro Phe Asn Gly Leu Asp Lys Gly Glu Pro
50 55 60
Val Ser Ala Ala Asp Ala Ala Ala Leu Glu His Asp Lys Ala Cys Asp
65 70 75 80
Gln Arg Leu Lys Ala Gly Asp Asn Pro Tyr Leu Arg Tyr Asn His Ala
85 90 95
Asp Ala Glu Phe Gln Glu Arg Leu Gln Glu Asp Thr Ser Phe Gly Gly
100 105 110
Asn Leu Gly Arg Ala Val Phe Gln Ala Lys Lys Arg Val Leu Glu Pro
115 120 125
Leu Gly Leu Val Glu Glu Ala Ala Lys Thr Ala Pro Gly Lys Lys Gly
130 135 140
Pro Val Glu Pro Ser Pro Gln Arg Ser Pro Asp Ser Ser Thr Gly Ile
145 150 155 160
Gly Lys Lys Gly Gln Gln Pro Ala Arg Lys Arg Leu Asn Phe Gly Gln
165 170 175
Thr Gly Asp Ser Glu Ser Val Pro Asp Pro Gln Pro Ile Gly Glu Pro
180 185 190
Pro Ala Gly Pro Ser Gly Leu Gly Ser Gly Thr Met Ala Ala Gly Gly
195 200 205
Gly Ala Pro Met Ala Asp Asn Asn Glu Gly Ala Asp Gly Val Gly Asn
210 215 220
Ala Ser Gly Asn Trp His Cys Asp Ser Thr Trp Leu Gly Asp Arg Val
225 230 235 240
Ile Thr Thr Ser Thr Arg Thr Trp Ala Leu Pro Thr Tyr Asn Asn His
245 250 255
Leu Tyr Lys Gln Ile Ser Ser Gln Ser Ala Gly Ser Thr Asn Asp Asn
260 265 270
Val Tyr Phe Gly Tyr Ser Thr Pro Trp Gly Tyr Phe Asp Phe Asn Arg
275 280 285
Phe His Cys His Phe Ser Pro Arg Asp Trp Gln Arg Leu Ile Asn Asn
290 295 300
Asn Trp Gly Phe Arg Pro Lys Lys Leu Asn Phe Lys Leu Phe Asn Ile
305 310 315 320
Gln Val Lys Glu Val Thr Thr Asn Asp Gly Val Thr Thr Ile Ala Asn
325 330 335
Asn Leu Thr Ser Thr Val Gln Val Phe Ser Asp Ser Glu Tyr Gln Leu
340 345 350
Pro Tyr Val Leu Gly Ser Ala His Gln Gly Cys Leu Pro Pro Phe Pro
355 360 365
Ala Asp Val Phe Met Ile Pro Gln Tyr Gly Tyr Leu Thr Leu Asn Asn
370 375 380
Gly Ser Gln Ser Val Gly Arg Ser Ser Phe Tyr Cys Leu Glu Tyr Phe
385 390 395 400
Pro Ser Gln Met Leu Arg Thr Gly Asn Asn Phe Thr Phe Ser Tyr Thr
405 410 415
Phe Glu Asp Val Pro Phe His Ser Ser Tyr Ala His Ser Gln Ser Leu
420 425 430
Asp Arg Leu Met Asn Pro Leu Ile Asp Gln Tyr Leu Tyr Tyr Leu Ala
435 440 445
Arg Thr Gln Ser Asn Ala Gly Gly Thr Ala Gly Asn Arg Glu Leu Gln
450 455 460
Phe Tyr Gln Gly Gly Pro Thr Thr Met Ala Glu Gln Ala Lys Asn Trp
465 470 475 480
Leu Pro Gly Pro Cys Phe Arg Gln Gln Arg Val Ser Lys Thr Leu Asp
485 490 495
Gln Asn Asn Asn Ser Asn Phe Ala Trp Thr Gly Ala Thr Lys Tyr His
500 505 510
Leu Asn Gly Arg Asn Ser Leu Val Asn Pro Gly Val Ala Met Ala Thr
515 520 525
His Lys Asp Asp Glu Glu Arg Phe Phe Pro Ser Ser Gly Val Leu Ile
530 535 540
Phe Gly Lys Thr Gly Ala Ala Asn Lys Thr Thr Leu Glu Asn Val Leu
545 550 555 560
Met Thr Asn Glu Glu Glu Ile Arg Pro Thr Asn Pro Val Ala Thr Glu
565 570 575
Glu Tyr Gly Thr Val Ser Ser Asn Leu Gln Ala Ala Asn Thr Ala Ala
580 585 590
Gln Thr Gln Val Val Asn Asn Gln Gly Ala Leu Pro Gly Met Val Trp
595 600 605
Gln Asn Arg Asp Val Tyr Leu Gln Gly Pro Ile Trp Ala Lys Ile Pro
610 615 620
His Thr Asp Gly Asn Phe His Pro Ser Pro Leu Met Gly Gly Phe Gly
625 630 635 640
Leu Lys His Pro Pro Pro Gln Ile Leu Ile Lys Asn Thr Pro Val Pro
645 650 655
Ala Asn Pro Pro Glu Val Phe Thr Pro Ala Lys Phe Ala Ser Phe Ile
660 665 670
Thr Gln Tyr Ser Thr Gly Gln Val Ser Val Glu Ile Glu Trp Glu Leu
675 680 685
Gln Lys Glu Asn Ser Lys Arg Trp Asn Pro Glu Ile Gln Tyr Thr Ser
690 695 700
Asn Phe Asp Glu Gln Thr Gly Val Asp Phe Ala Val Asp Ser Gln Gly
705 710 715 720
Val Tyr Ser Glu Pro Arg Pro Ile Gly Thr Arg Tyr Leu Thr Arg Asn
725 730 735
Leu
<210>112
<211>737
<212>PRT
<213>vp1,克隆rh.65
<400>112
Met Ala Ala Asp Gly Tyr Leu Pro Asp Trp Leu Glu Asp Asn Leu Ser
1 5 10 15
Glu Gly Ile Arg Glu Trp Trp Asp Leu Lys Pro Gly Ala Pro Lys Pro
20 25 30
Lys Ala Asn Gln Gln Lys Gln Asp Asp Gly Arg Gly Leu Val Leu Pro
35 40 45
Gly Tyr Lys Tyr Leu Gly Pro Phe Asn Gly Leu Asp Lys Gly Glu Pro
50 55 60
Val Asn Ala Ala Asp Ala Ala Ala Leu Glu His Asp Lys Ala Tyr Asp
65 70 75 80
Gln Gln Leu Lys Ala Gly Asp Asn Pro Tyr Leu Arg Tyr Asn His Ala
85 90 95
Asp Ala Glu Phe Gln Glu Arg Leu Gln Glu Asp Thr Ser Phe Gly Gly
100 105 110
Asn Leu Gly Arg Ala Val Phe Gln Ala Lys Lys Arg Val Leu Glu Pro
115 120 125
Leu Gly Leu Val Glu Glu Ala Ala Lys Thr Ala Pro Gly Lys Lys Arg
130 135 140
Pro Val Glu Pro Ser Pro Gln Arg Ser Pro Asp Ser Ser Thr Gly Ile
145 150 155 160
Gly Lys Lys Gly Gln Gln Pro Ala Arg Lys Arg Leu Asn Phe Gly Gln
165 170 175
Thr Gly Asp Ser Glu Ser Val Pro Asp Pro Gln Pro Ile Gly Glu Pro
180 185 190
Pro Ala Gly Pro Ser Gly Leu Gly Ser Gly Thr Met Ala Ala Gly Gly
195 200 205
Gly Ala Pro Met Ala Asp Asn Asn Glu Gly Ala Asp Gly Val Gly Asn
210 215 220
Ala Ser Gly Asn Trp His Cys Asp Ser Thr Trp Leu Gly Asp Arg Val
225 230 235 240
Ile Thr Thr Ser Thr Arg Thr Trp Ala Leu Pro Thr Tyr Asn Asn His
245 250 255
Leu Tyr Lys Gln Ile Ser Ser Gln Ser Ala Gly Ser Thr Asn Asp Asn
260 265 270
Val Tyr Phe Gly Tyr Ser Thr Pro Trp Gly Tyr Phe Asp Phe Asn Arg
275 280 285
Phe His Cys His Phe Ser Pro Arg Asp Trp Gln Arg Leu Ile Asn Asn
290 295 300
Asn Trp Gly Phe Arg Pro Lys Lys Leu Asn Phe Lys Leu Phe Asn Ile
305 310 315 320
Gln Val Lys Glu Val Thr Thr Asn Asp Gly Val Thr Thr Ile Ala Asn
325 330 335
Asn Leu Thr Ser Thr Val Gln Val Phe Ser Asp Ser Glu Tyr Gln Leu
340 345 350
Pro Tyr Val Leu Gly Ser Ala His Gln Gly Cys Leu Pro Pro Phe Pro
355 360 365
Ala Asp Val Phe Met Ile Pro Gln Tyr Gly Tyr Leu Thr Leu Asn Asn
370 375 380
Gly Ser Gln Ser Val Gly Arg Ser Ser Phe Tyr Cys Leu Glu Tyr Phe
385 390 395 400
Pro Ser Gln Met Leu Arg Thr Gly Asn Asn Phe Thr Phe Ser Tyr Thr
405 410 415
Phe Glu Asp Val Pro Phe His Ser Ser Tyr Ala His Ser Gln Ser Leu
420 425 430
Asp Arg Leu Met Asn Pro Leu Ile Asp Gln Tyr Leu Tyr Tyr Leu Ala
435 440 445
Arg Thr Gln Ser Asn Ala Gly Gly Thr Ala Gly Asn Arg Glu Leu Gln
450 455 460
Phe Tyr Gln Gly Gly Pro Thr Thr Met Ala Glu Gln Ala Lys Asn Trp
465 470 475 480
Leu Pro Arg Pro Cys Phe Arg Gln Gln Arg Val Ser Lys Thr Leu Asp
485 490 495
Gln Asn Asn Asn Ser Asn Phe Ala Trp Thr Gly Ala Thr Lys Tyr His
500 505 510
Leu Asn Gly Arg Asn Ser Leu Val Asn Pro Gly Val Ala Met Ala Thr
515 520 525
His Lys Asp Asp Glu Glu Arg Phe Phe Pro Ser Ser Gly Val Leu Ile
530 535 540
Phe Gly Lys Thr Gly Ala Ala Asn Lys Thr Thr Leu Glu Asn Val Leu
545 550 555 560
Met Thr Asn Glu Glu Glu Ile Arg Pro Thr Asn Pro Val Ala Thr Glu
565 570 575
Glu Tyr Gly Thr Val Ser Ser Asn Leu Gln Ala Ala Asn Thr Ala Ala
580 585 590
Gln Thr Gln Val Val Asn Asn Gln Gly Ala Leu Pro Gly Met Val Trp
595 600 605
Gln Asn Arg Asp Val Tyr Leu Gln Gly Pro Ile Trp Ala Lys Ile Pro
610 615 620
His Thr Asp Gly Asn Phe His Pro Ser Pro Leu Met Gly Gly Phe Gly
625 630 635 640
Leu Lys His Pro Pro Pro Gln Ile Leu Ile Lys Asn Thr Pro Val Pro
645 650 655
Ala Asn Pro Pro Glu Val Phe Thr Pro Ala Lys Phe Ala Ser Phe Ile
660 665 670
Thr Gln Tyr Ser Thr Gly Gln Val Ser Val Glu Ile Glu Trp Glu Leu
675 680 685
Gln Lys Glu Asn Ser Lys Arg Trp Asn Pro Glu Ile Gln Tyr Thr Ser
690 695 700
Asn Phe Asp Lys Gln Thr Gly Val Asp Phe Ala Val Asp Ser Gln Gly
705 710 715 720
Val Tyr Ser Glu Pro Arg Pro Ile Gly Thr Arg Tyr Leu Thr Arg Asn
725 730 735
Leu
<210>113
<211>737
<212>PRT
<213>vp1,克隆rh.67
<400>113
Met Ala Ala Asp Gly Tyr Leu Pro Asp Trp Leu Glu Asp Asn Leu Ser
1 5 10 15
Glu Gly Ile Arg Glu Trp Trp Asp Leu Lys Pro Gly Ala Pro Lys Pro
20 25 30
Lys Ala Asn Gln Gln Lys Gln Asp Asp Gly Arg Gly Leu Val Leu Pro
35 40 45
Gly Tyr Lys Tyr Leu Gly Pro Phe Asn Gly Leu Asp Lys Gly Glu Pro
50 55 60
Val Asn Ala Ala Asp Ala Ala Ala Leu Glu His Asp Lys Ala Tyr Asp
65 70 75 80
Gln Gln Leu Lys Ala Gly Asp Asn Pro Tyr Leu Arg Tyr Asn His Ala
85 90 95
Asp Ala Glu Phe Gln Glu Arg Leu Gln Glu Asp Thr Ser Phe Gly Gly
100 105 110
Asn Leu Gly Arg Ala Val Leu Gln Ala Lys Lys Arg Val Leu Glu Pro
115 120 125
Leu Gly Leu Val Glu Glu Ala Ala Lys Thr Ala Pro Gly Lys Lys Arg
130 135 140
Pro Val Glu Pro Ser Pro Gln Arg Ser Pro Asp Ser Ser Thr Gly Ile
145 150 155 160
Gly Lys Lys Gly Gln Gln Pro Ala Arg Lys Arg Leu Asn Phe Gly Gln
165 170 175
Thr Gly Asp Ser Glu Ser Val Pro Asp Pro Gln Pro Ile Gly Glu Pro
180 185 190
Pro Ala Gly Pro Ser Gly Leu Gly Ser Gly Thr Met Ala Ala Gly Gly
195 200 205
Gly Ala Pro Met Ala Asp Asn Asn Glu Gly Ala Asp Gly Val Gly Asn
210 215 220
Ala Ser Gly Asn Trp His Cys Asp Ser Thr Trp Leu Gly Asp Arg Val
225 230 235 240
Ile Thr Thr Ser Thr Arg Thr Trp Ala Leu Pro Thr Tyr Asn Asn His
245 250 255
Leu Tyr Lys Gln Ile Ser Ser Gln Ser Ala Gly Ser Thr Asn Asp Asn
260 265 270
Val Tyr Phe Gly Tyr Ser Thr Pro Trp Gly Tyr Phe Asp Phe Asn Arg
275 280 285
Phe His Cys His Phe Ser Pro Arg Asp Trp Gln Arg Leu Ile Asn Asn
290 295 300
Asn Trp Gly Phe Arg Pro Lys Lys Leu Asn Phe Lys Leu Phe Asn Ile
305 310 315 320
Gln Val Lys Glu Val Thr Thr Asn Asp Gly Val Thr Thr Ile Ala Asn
325 330 335
Asn Leu Thr Ser Thr Val Gln Val Phe Ser Asp Ser Glu Tyr Gln Leu
340 345 350
Pro Tyr Val Leu Gly Ser Ala His Gln Gly Cys Leu Pro Pro Phe Pro
355 360 365
Ala Asp Val Phe Met Ile Pro Gln Tyr Gly Tyr Leu Thr Leu Asn Asn
370 375 380
Gly Ser Gln Ser Val Gly Arg Ser Ser Phe Tyr Cys Leu Glu Tyr Phe
385 390 395 400
Pro Ser Gln Met Leu Arg Thr Gly Asn Asn Phe Thr Phe Ser Tyr Thr
405 410 415
Phe Glu Asp Val Pro Phe His Ser Ser Tyr Ala His Ser Gln Ser Leu
420 425 430
Asp Arg Leu Met Asn Pro Leu Ile Asp Gln Tyr Leu Tyr Tyr Leu Ala
435 440 445
Arg Thr Gln Ser Asn Ala Gly Gly Thr Ala Gly Asn Arg Glu Leu Gln
450 455 460
Phe Tyr Gln Gly Gly Pro Thr Thr Met Ala Glu Gln Ala Lys Asn Trp
465 470 475 480
Leu Pro Gly Pro Cys Phe Arg Gln Gln Arg Val Ser Lys Thr Leu Asp
485 490 495
Gln Asn Asn Asn Ser Asn Phe Ala Trp Thr Gly Ala Thr Lys Tyr His
500 505 510
Leu Asn Gly Arg Asn Ser Leu Val Asn Pro Gly Val Ala Met Ala Thr
515 520 525
His Lys Asp Asp Glu Glu Arg Phe Phe Pro Ser Ser Gly Val Leu Ile
530 535 540
Phe Gly Lys Thr Gly Ala Ala Asn Lys Thr Thr Leu Glu Asn Val Leu
545 550 555 560
Met Thr Asn Glu Glu Glu Ile Arg Pro Thr Asn Pro Val Ala Thr Glu
565 570 575
Glu Tyr Gly Thr Val Ser Ser Asn Leu Gln Ala Ala Asn Thr Ala Ala
580 585 590
Gln Thr Gln Val Val Asn Asn Gln Gly Ala Leu Pro Gly Met Val Trp
595 600 605
Gln Asn Arg Asp Val Tyr Leu Gln Gly Pro Ile Trp Ala Lys Ile Pro
610 615 620
His Thr Asp Gly Asn Phe His Pro Ser Pro Leu Met Gly Gly Phe Gly
625 630 635 640
Leu Lys His Pro Pro Pro Gln Ile Leu Ile Lys Asn Thr Pro Val Pro
645 650 655
Ala Asn Pro Pro Glu Val Phe Thr Pro Ala Lys Phe Ala Ser Phe Ile
660 665 670
Thr Gln Tyr Ser Thr Gly Gln Val Ser Val Glu Ile Glu Trp Glu Leu
675 680 685
Gln Lys Glu Asn Ser Lys Arg Trp Asn Pro Glu Ile Gln Tyr Thr Ser
690 695 700
Asn Phe Asp Lys Gln Thr Gly Val Asp Phe Ala Val Asp Ser Gln Gly
705 710 715 720
Val Tyr Ser Glu Pro Arg Pro Ile Gly Thr Arg Tyr Leu Thr Arg Asn
725 730 735
Leu
<210>114
<211>737
<212>PRT
<213>vp1,克隆rh.62
<400>114
Met Ala Ala Asp Gly Tyr Leu Pro Asp Trp Leu Glu Asp Asn Leu Ser
1 5 10 15
Glu Gly Ile Arg Glu Trp Trp Asp Leu Lys Pro Gly Ala Pro Lys Pro
20 25 30
Lys Ala Asn Gln Gln Lys Gln Asp Asp Gly Arg Gly Leu Val Leu Pro
35 40 45
Gly Tyr Lys Tyr Leu Gly Pro Phe Asn Gly Leu Asp Lys Gly Glu Pro
50 55 60
Val Asn Ala Ala Asp Ala Ala Ala Leu Glu His Asp Lys Ala Tyr Asp
65 70 75 80
Gln Gln Leu Lys Ala Gly Asp Asn Pro Tyr Leu Arg Tyr Asn His Ala
85 90 95
Asp Ala Glu Phe Gln Glu Arg Leu Gln Glu Asp Thr Ser Phe Gly Gly
100 105 110
Asn Leu Gly Arg Ala Val Phe Gln Ala Lys Lys Arg Val Leu Glu Pro
115 120 125
Leu Gly Leu Ala Glu Glu Ala Ala Lys Thr Ala Pro Gly Lys Lys Arg
130 135 140
Pro Val Glu Pro Ser Pro Gln Arg Ser Pro Asp Ser Ser Thr Gly Ile
145 150 155 160
Gly Lys Lys Gly Gln Gln Pro Ala Arg Lys Arg Leu Asn Phe Gly Gln
165 170 175
Thr Gly Asp Ser Glu Ser Val Pro Asp Pro Gln Pro Ile Gly Glu Pro
180 185 190
Pro Ala Gly Pro Ser Gly Leu Gly Ser Gly Thr Met Ala Ala Gly Gly
195 200 205
Gly Ala Pro Met Ala Asp Asn Asn Lys Gly Ala Asp Gly Val Gly Asn
210 215 220
Ala Ser Gly Asn Trp His Cys Asp Ser Thr Trp Leu Gly Asp Arg Val
225 230 235 240
Ile Thr Thr Ser Thr Arg Thr Trp Ala Leu Pro Thr Tyr Asn Asn His
245 250 255
Leu Tyr Lys Gln Ile Ser Ser Gln Ser Ala Gly Ser Thr Asn Asp Asn
260 265 270
Val Tyr Phe Gly Tyr Ser Thr Pro Trp Gly Tyr Phe Asp Phe Asn Arg
275 280 285
Phe His Cys His Phe Ser Pro Arg Asp Trp Gln Arg Leu Ile Asn Asn
290 295 300
Asn Trp Gly Phe Arg Pro Lys Lys Leu Asn Phe Lys Leu Phe Asn Ile
305 310 315 320
Gln Val Lys Glu Val Thr Thr Gly Asp Gly Val Thr Thr Ile Ala Asn
325 330 335
Asn Leu Thr Ser Thr Val Gln Val Phe Ser Asp Ser Glu Tyr Gln Leu
340 345 350
Pro Tyr Val Leu Gly Ser Ala His Gln Gly Cys Leu Pro Pro Phe Pro
355 360 365
Ala Asp Val Phe Met Ile Pro Gln Tyr Gly Tyr Leu Thr Leu Asn Asn
370 375 380
Asp Ser Gln Ser Val Gly Arg Ser Ser Phe Tyr Cys Leu Glu Tyr Phe
385 390 395 400
Pro Ser Gln Met Leu Arg Thr Gly Asn Asn Phe Thr Phe Ser Tyr Thr
405 410 415
Phe Glu Asp Val Pro Phe His Ser Ser Tyr Ala His Ser Gln Ser Leu
420 425 430
Asp Arg Leu Met Asn Pro Leu Ile Asp Gln Tyr Leu Tyr Tyr Leu Ala
435 440 445
Arg Thr Gln Ser Asn Ala Gly Gly Thr Ala Gly Asn Arg Glu Leu Gln
450 455 460
Phe Tyr Gln Gly Gly Pro Thr Thr Met Ala Glu Gln Ala Lys Asn Trp
465 470 475 480
Leu Pro Gly Pro Cys Phe Arg Gln Gln Arg Val Ser Lys Thr Leu Asp
485 490 495
Gln Asn Asn Asn Ser Asn Phe Ala Trp Thr Gly Ala Thr Lys Tyr His
500 505 510
Leu Asn Gly Arg Asn Ser Leu Val Asn Pro Gly Val Ala Met Ala Thr
515 520 525
His Lys Asp Asp Glu Glu Arg Phe Phe Pro Ser Ser Gly Val Leu Ile
530 535 540
Phe Gly Lys Thr Gly Ala Ala Asn Lys Thr Thr Leu Glu Asn Val Leu
545 550 555 560
Met Thr Asn Glu Glu Glu Ile Arg Pro Thr Asn Pro Val Ala Thr Glu
565 570 575
Glu Tyr Gly Thr Val Ser Ser Asn Leu Gln Ala Ala Asn Thr Ala Ala
580 585 590
Gln Thr Gln Val Val Asn Asn Gln Gly Ala Leu Pro Gly Met Val Trp
595 600 605
Gln Asn Arg Asp Val Tyr Leu Gln Gly Pro Ile Trp Ala Lys Ile Pro
610 615 620
His Thr Asp Gly Asn Phe His Pro Ser Pro Leu Met Gly Gly Phe Gly
625 630 635 640
Leu Lys His Pro Pro Pro Gln Ile Leu Ile Lys Asn Thr Pro Val Pro
645 650 655
Ala Asn Pro Pro Glu Val Phe Thr Pro Ala Lys Phe Ala Ser Phe Ile
660 665 670
Thr Gln Tyr Ser Thr Gly Gln Val Ser Val Glu Ile Glu Trp Glu Leu
675 680 685
Gln Lys Glu Asn Ser Lys Arg Trp Asn Pro Glu Ile Gln Tyr Thr Ser
690 695 700
Asn Phe Asp Lys Gln Thr Gly Val Asp Phe Ala Val Asp Ser Gln Gly
705 710 715 720
Val Tyr Ser Glu Pro Arg Pro Ile Gly Thr Arg Tyr Leu Thr Arg Asn
725 730 735
Leu
<210>115
<211>737
<212>PRT
<213>vp1,克隆rh.48
<400>115
Met Ala Ala Asp Gly Tyr Leu Pro Asp Trp Leu Glu Asp Asn Leu Ser
1 5 10 15
Glu Gly Ile Arg Glu Trp Trp Asp Leu Lys Pro Gly Ala Pro Lys Pro
20 25 30
Lys Ala Asn Gln Gln Lys Gln Asp Asp Gly Arg Gly Leu Val Leu Pro
35 40 45
Gly Tyr Lys Tyr Leu Gly Pro Phe Asn Gly Leu Asp Lys Gly Glu Pro
50 55 60
Val Asn Ala Ala Asp Ala Ala Ala Leu Glu His Asp Lys Ala Tyr Asp
65 70 75 80
Gln Gln Leu Lys Ala Gly Asp Asn Pro Tyr Leu Arg Tyr Asn His Ala
85 90 95
Asp Ala Glu Phe Gln Glu Arg Leu Gln Glu Asp Thr Ser Phe Gly Gly
100 105 110
Asn Leu Gly Arg Ala Val Phe Gln Ala Lys Lys Arg Val Leu Glu Pro
115 120 125
Leu Gly Leu Val Glu Glu Ala Ala Lys Thr Ala Pro Gly Lys Lys Arg
130 135 140
Pro Val Glu Pro Ser Pro Gln Arg Ser Pro Asp Ser Ser Thr Gly Ile
145 150 155 160
Gly Lys Lys Gly Gln Gln Pro Ala Arg Lys Arg Leu Asn Phe Gly Gln
165 170 175
Thr Gly Asp Ser Glu Ser Val Pro Asp Pro Gln Pro Ile Gly Glu Pro
180 185 190
Pro Ala Gly Pro Ser Gly Leu Gly Ser Gly Thr Met Ala Ala Gly Gly
195 200 205
Gly Ala Pro Met Ala Asp Asn Asn Lys Gly Ala Asp Gly Val Gly Asn
210 215 220
Ala Ser Gly Asn Trp His Cys Asp Ser Thr Trp Leu Gly Asp Arg Val
225 230 235 240
Ile Thr Thr Ser Thr Arg Thr Trp Ala Leu Pro Thr Tyr Asn Asn His
245 250 255
Leu Tyr Lys Gln Ile Ser Ser Gln Ser Ala Gly Ser Thr Asn Asp Asn
260 265 270
Val Tyr Phe Gly Tyr Ser Thr Pro Trp Gly Tyr Phe Asp Phe Asn Arg
275 280 285
Phe His Cys His Phe Ser Pro Arg Asp Trp Gln Arg Leu Ile Asn Ser
290 295 300
Asn Trp Gly Phe Arg Pro Lys Lys Leu Asn Phe Lys Leu Phe Asn Ile
305 310 315 320
Gln Val Lys Glu Val Thr Thr Asn Asp Gly Val Thr Thr Ile Ala Asn
325 330 335
Asn Leu Thr Ser Thr Val Gln Val Phe Ser Asp Ser Glu Tyr Gln Leu
340 345 350
Pro Tyr Val Leu Gly Ser Ala His Gln Gly Cys Leu Pro Pro Phe Pro
355 360 365
Ala Asp Val Phe Met Ile Pro Gln Tyr Gly Tyr Leu Thr Leu Asn Asn
370 375 380
Gly Ser Gln Ser Val Gly Arg Ser Ser Phe Tyr Cys Leu Glu Tyr Phe
385 390 395 400
Pro Ser Gln Met Leu Arg Thr Gly Asn Asn Phe Thr Phe Ser Tyr Thr
405 410 415
Phe Glu Asp Val Pro Phe His Ser Ser Tyr Ala His Ser Gln Ser Leu
420 425 430
Asp Arg Leu Met Asn Pro Leu Ile Asp Gln Tyr Leu Tyr Tyr Leu Ala
435 440 445
Arg Thr Gln Ser Asn Ala Gly Gly Thr Ala Gly Asn Arg Glu Leu Gln
450 455 460
Phe Tyr Gln Gly Gly Pro Thr Thr Met Ala Glu Gln Ala Lys Asn Trp
465 470 475 480
Leu Pro Gly Pro Cys Phe Arg Gln Gln Arg Val Ser Lys Thr Leu Asp
485 490 495
Gln Asn Asn Asn Ser Asn Phe Ala Trp Thr Gly Ala Thr Lys Tyr His
500 505 510
Leu Asn Gly Arg Asn Ser Leu Val Asn Pro Gly Val Ala Met Ala Thr
515 520 525
His Lys Asp Asp Glu Glu Arg Phe Phe Pro Ser Ser Gly Val Leu Ile
530 535 540
Phe Gly Lys Thr Gly Ala Ala Asn Lys Thr Thr Leu Glu Asn Val Leu
545 550 555 560
Met Thr Asn Glu Glu Glu Ile Arg Pro Thr Asn Pro Val Ala Thr Glu
565 570 575
Glu Tyr Gly Thr Val Ser Ser Asn Leu Gln Ala Ala Asn Thr Ala Ala
580 585 590
Gln Thr Gln Val Val Asn Asn Gln Gly Ala Leu Pro Gly Met Val Trp
595 600 605
Gln Asn Arg Asp Val Tyr Leu Gln Gly Pro Ile Trp Ala Lys Ile Pro
610 615 620
His Thr Asp Gly Asn Phe His Pro Ser Pro Leu Met Gly Gly Phe Gly
625 630 635 640
Leu Lys His Pro Pro Pro Gln Ile Leu Ile Lys Asn Thr Pro Val Pro
645 650 655
Ala Asn Pro Pro Glu Val Phe Thr Pro Ala Lys Phe Ala Ser Phe Ile
660 665 670
Thr Gln Tyr Ser Thr Gly Gln Val Ser Val Glu Ile Glu Trp Glu Leu
675 680 685
Gln Lys Glu Asn Ser Lys Arg Trp Asn Pro Glu Ile Gln Tyr Thr Ser
690 695 700
Asn Phe Asp Lys Gln Thr Gly Val Asp Phe Ala Val Asp Ser Gln Gly
705 710 715 720
Val Tyr Ser Glu Pro Arg Pro Ile Gly Thr Arg Tyr Leu Thr Arg Asn
725 730 735
Leu
<210>116
<211>737
<212>PRT
<213>vp1,克隆rh.54
<400>116
Met Ala Ala Asp Gly Tyr Leu Pro Asp Trp Leu Glu Asp Asn Leu Ser
1 5 10 15
Glu Gly Ile Arg Glu Trp Trp Asp Leu Lys Pro Gly Ala Pro Lys Pro
20 25 30
Lys Ala Asn Gln Gln Lys Gln Asp Asp Gly Arg Gly Leu Val Leu Pro
35 40 45
Gly Tyr Lys Tyr Leu Gly Pro Phe Asn Gly Leu Asp Lys Gly Glu Pro
50 55 60
Val Asn Ala Ala Asp Ala Ala Ala Leu Glu His Asp Lys Ala Tyr Asp
65 70 75 80
Gln Gln Leu Lys Ala Gly Asp Asn Pro Tyr Leu Arg Tyr Asn His Ala
85 90 95
Asp Ala Glu Phe Gln Glu Arg Leu Gln Glu Asp Thr Ser Phe Gly Gly
100 105 110
Asn Leu Gly Arg Ala Val Phe Gln Ala Lys Lys Arg Val Leu Glu Pro
115 120 125
Leu Gly Leu Val Glu Glu Ala Ala Lys Thr Ala Pro Gly Lys Lys Arg
130 135 140
Pro Val Glu Pro Ser Pro Gln Arg Ser Pro Asp Ser Ser Thr Gly Ile
145 150 155 160
Gly Lys Lys Gly Gln Gln Pro Ala Arg Lys Arg Leu Asn Phe Gly Gln
165 170 175
Thr Gly Asp Ser Glu Ser Val Pro Asp Pro Gln Pro Leu Gly Glu Pro
180 185 190
Pro Ala Gly Pro Ser Gly Leu Gly Ser Gly Thr Met Ala Ala Gly Gly
195 200 205
Gly Ala Pro Met Ala Asp Asn Asn Glu Gly Ala Asp Gly Val Gly Asn
210 215 220
Ala Ser Gly Asn Trp His Cys Asp Ser Thr Trp Leu Gly Asp Arg Val
225 230 235 240
Ile Thr Thr Ser Thr Arg Thr Trp Ala Leu Pro Thr Tyr Asn Asn His
245 250 255
Leu Tyr Lys Gln Ile Ser Ser Gln Ser Ala Gly Ser Thr Asn Asp Asn
260 265 270
Val Tyr Phe Gly Tyr Ser Thr Pro Trp Gly Tyr Phe Asp Phe Asn Arg
275 280 285
Phe His Cys His Phe Ser Pro Arg Asp Trp Gln Arg Leu Ile Asn Asn
290 295 300
Asn Trp Gly Phe Arg Pro Lys Lys Leu Asn Phe Lys Leu Phe Asn Ile
305 310 315 320
Gln Val Lys Glu Val Thr Thr Asn Asp Gly Val Thr Thr Ile Ala Asn
325 330 335
Asn Leu Thr Ser Thr Val Gln Val Phe Ser Asp Ser Glu Tyr Gln Leu
340 345 350
Pro Tyr Val Leu Gly Ser Ala His Gln Gly Cys Leu Pro Pro Phe Pro
355 360 365
Ala Asp Val Phe Met Ile Pro Gln Tyr Gly Tyr Leu Thr Leu Asn Asn
370 375 380
Gly Ser Gln Ser Val Gly Arg Ser Ser Phe Tyr Cys Leu Glu Tyr Phe
385 390 395 400
Pro Ser Gln Val Leu Arg Thr Gly Asn Asn Phe Thr Phe Ser Tyr Thr
405 410 415
Phe Glu Asp Val Pro Phe His Ser Ser Tyr Ala His Ser Gln Ser Leu
420 425 430
Asp Arg Leu Met Asn Pro Leu Ile Asp Gln Tyr Leu Tyr Tyr Leu Ala
435 440 445
Arg Thr Gln Ser Asn Pro Gly Gly Thr Ser Gly Asn Arg Glu Leu Gln
450 455 460
Phe Tyr Gln Gly Gly Pro Ser Thr Met Ala Glu Gln Ala Lys Asn Trp
465 470 475 480
Leu Pro Gly Pro Cys Phe Arg Gln Gln Arg Val Ser Lys Thr Leu Asp
485 490 495
Gln Asn Asn Asn Ser Asn Phe Ala Trp Thr Gly Ala Thr Lys Tyr His
500 505 510
Leu Asn Gly Arg Asn Ser Leu Val Asn Pro Gly Val Ala Met Ala Thr
515 520 525
His Lys Asp Asp Glu Asp Arg Phe Phe Pro Ser Ser Gly Val Leu Ile
530 535 540
Phe Gly Lys Thr Gly Ala Thr Asn Lys Thr Thr Leu Glu Asn Val Leu
545 550 555 560
Met Thr Asn Glu Glu Glu Ile Arg Pro Thr Asn Pro Val Ala Thr Glu
565 570 575
Glu Tyr Gly Ile Val Ser Ser Asn Leu Gln Ala Ala Asn Thr Ala Ala
580 585 590
Gln Thr Gln Val Val Asn Asn Gln Gly Ala Leu Pro Gly Met Val Trp
595 600 605
Gln Asn Arg Asp Val Tyr Leu Gln Gly Pro Ile Trp Ala Lys Ile Pro
610 615 620
His Thr Asp Gly Asn Phe His Pro Ser Pro Leu Met Gly Gly Phe Gly
625 630 635 640
Leu Lys His Pro Pro Pro Gln Ile Leu Ile Lys Asn Thr Pro Val Pro
645 650 655
Ala Asn Pro Pro Glu Val Phe Thr Pro Ala Lys Phe Ala Ser Phe Ile
660 665 670
Thr Gln Tyr Ser Thr Gly Gln Val Ser Val Glu Ile Glu Trp Glu Leu
675 680 685
Gln Lys Glu Asn Ser Lys Arg Trp Asn Pro Glu Ile Gln Tyr Thr Ser
690 695 700
Asn Phe Asp Lys Gln Thr Gly Val Asp Phe Ala Val Asp Ser Gln Gly
705 710 715 720
Val Tyr Ser Glu Pro Arg Pro Ile Gly Thr Arg Tyr Leu Thr Arg Asn
725 730 735
Leu
<210>117
<211>737
<212>PRT
<213>vp1,克隆rh.55
<400>117
Met Ala Ala Asp Gly Tyr Leu Pro Asp Trp Leu Glu Asp Asn Leu Ser
1 5 10 15
Glu Gly Ile Arg Glu Trp Trp Asp Leu Lys Pro Gly Ala Pro Lys Pro
20 25 30
Lys Ala Asn Gln Gln Lys Gln Asp Asp Gly Arg Gly Leu Val Leu Pro
35 40 45
Gly Tyr Lys Tyr Leu Gly Pro Phe Asn Gly Leu Asp Lys Gly Glu Pro
50 55 60
Val Asn Ala Ala Asp Ala Ala Ala Leu Glu His Asp Lys Ala Tyr Asp
65 70 75 80
Gln Gln Leu Lys Ala Gly Asp Asr Pro Tyr Leu Arg Tyr Asn His Ala
85 90 95
Asp Ala Glu Phe Gln Glu Arg Leu Gln Glu Asp Thr Ser Phe Gly Gly
100 105 110
Asn Leu Gly Arg Ala Val Phe Gln Ala Lys Lys Arg Val Leu Glu Pro
115 120 125
Leu Gly Leu Val Glu Glu Gly Ala Lys Thr Ala Pro Gly Lys Lys Arg
130 135 140
Pro Val Glu Pro Ser Pro Gln Arg Ser Pro Asp Ser Ser Thr Gly Ile
145 150 155 160
Gly Lys Lys Gly Gln Gln Pro Ala Arg Lys Arg Leu Asn Phe Gly Gln
165 170 175
Thr Gly Asp Ser Glu Ser Val Pro Asp Pro Gln Pro Ile Gly Glu Pro
180 185 190
Pro Ala Ala Pro Ser Ser Val Gly Ser Gly Thr Met Ala Ala Gly Gly
195 200 205
Gly Ala Pro Met Ala Asp Asn Asn Glu Gly Ala Asp Gly Val Gly Ser
210 215 220
Ser Ser Gly Asn Trp His Cys Asp Ser Thr Arg Leu Gly Asp Arg Val
225 230 235 240
Ile Thr Thr Ser Thr Arg Thr Trp Ala Leu Pro Thr Tyr Asn Asn His
245 250 255
Leu Tyr Lys Gln Ile Ser Ser Gln Ser Ala Gly Ser Thr Asn Asp Asn
260 265 270
Val Tyr Phe Gly Tyr Ser Thr Pro Trp Gly Tyr Phe Asp Phe Asn Arg
275 280 285
Phe His Cys His Phe Ser Pro Arg Asp Trp Gln Arg Leu Ile Asn Asn
290 295 300
Asn Trp Gly Phe Arg Pro Lys Lys Leu Asn Phe Lys Leu Phe Asn Ile
305 310 315 320
Gln Val Lys Glu Val Thr Thr Asn Asp Gly Val Thr Thr Ile Ala Asn
325 330 335
Asn Leu Thr Ser Thr Val Gln Val Phe Ser Asp Ser Glu Tyr Gln Leu
340 345 350
Pro Tyr Val Leu Gly Ser Ala His Gln Gly Cys Leu Pro Pro Phe Pro
355 360 365
Ala Asp Val Phe Met Ile Pro Gln Tyr Gly Tyr Leu Thr Leu Asn Asn
370 375 380
Gly Ser Gln Ser Val Gly Arg Ser Ser Phe Tyr Cys Leu Glu Tyr Phe
385 390 395 400
Pro Ser Gln Met Leu Arg Thr Gly Asn Asn Phe Thr Phe Ser Tyr Thr
405 410 415
Phe Glu Asp Val Pro Phe His Ser Ser Tyr Ala His Ser Gln Ser Leu
420 425 430
Asp Arg Leu Met Asn Pro Leu Ile Asp Gln Tyr Leu Tyr Tyr Leu Ala
435 440 445
Arg Thr Gln Ser Asn Ala Gly Gly Thr Ala Gly Asn Arg Glu Leu Gln
450 455 460
Phe Tyr Gln Gly Gly Pro Thr Thr Met Ala Glu Gln Ala Lys Asn Trp
465 470 475 480
Leu Pro Gly Pro Cys Phe Arg Gln Arg Arg Val Ser Lys Thr Leu Asp
485 490 495
Gln Asn Asn Asn Ser Asn Phe Ala Trp Thr Gly Ala Thr Lys Tyr His
500 505 510
Leu Asn Gly Arg Asn Ser Leu Val Asn Pro Gly Val Ala Met Ala Thr
515 520 525
His Lys Asp Asp Glu Glu Arg Phe Phe Pro Ser Ser Gly Val Leu Ile
530 535 540
Phe Gly Lys Thr Gly Ala Ala Asn Lys Thr Thr Leu Glu Asn Val Leu
545 550 555 560
Met Thr Asn Glu Glu Glu Ile Arg Pro Thr Asn Pro Val Ala Thr Glu
565 570 575
Glu Tyr Gly Thr Val Ser Ser Asn Leu Gln Ala Ala Asn Thr Ala Ala
580 585 590
Gln Thr Gln Val Val Asn Asn Gln Gly Ala Leu Pro Gly Met Val Trp
595 600 605
Gln Asn Arg Asp Val Tyr Leu Gln Gly Pro Ile Trp Ala Lys Ile Pro
610 615 620
His Thr Asp Gly Asn Phe His Pro Ser Pro Leu Met Gly Gly Phe Gly
625 630 635 640
Leu Lys His Pro Pro Pro Gln Ile Leu Ile Lys Asn Thr Pro Val Pro
645 650 655
Ala Asn Pro Pro Glu Val Phe Thr Pro Ala Lys Phe Ala Ser Phe Ile
660 665 670
Thr Gln Tyr Ser Thr Gly Gln Val Ser Val Glu Ile Glu Trp Glu Leu
675 680 685
Gln Lys Glu Asn Ser Lys Arg Trp Asn Pro Glu Ile Gln Tyr Thr Ser
690 695 700
Asn Phe Asp Lys Gln Thr Gly Val Asp Phe Ala Val Asp Ser Gln Gly
705 710 715 720
Val Tyr Ser Glu Pro Arg Pro Ile Gly Thr Arg Tyr Leu Thr Arg Asn
725 730 735
Leu
<210>118
<211>738
<212>PRT
<213>vp1,克隆rh.47
<400>118
Met Ala Ala Asp Gly Tyr Leu Pro Asp Trp Leu Glu Asp Asn Leu Ser
1 5 10 15
Glu Gly Ile Arg Glu Trp Trp Asp Leu Lys Pro Gly Ala Pro Lys Pro
20 25 30
Lys Ala Asn Gln Gln Lys Gln Asp Asp Gly Arg Gly Leu Val Leu Pro
35 40 45
Gly Tyr Lys His Leu Gly Pro Phe Asn Gly Leu Asp Lys Gly Glu Pro
50 55 60
Val Asn Ala Ala Asp Ala Ala Ala Leu Glu His Asp Lys Ala Tyr Asp
65 70 75 80
Gln Gln Leu Lys Ala Gly Asp Asn Pro Tyr Leu Arg Tyr Asn His Ala
85 90 95
Asp Ala Glu Phe Gln Glu Arg Leu Gln Glu Asp Thr Ser Phe Gly Gly
100 105 110
Asn Leu Gly Arg Ala Val Phe Gln Ala Lys Lys Arg Val Leu Glu Pro
115 120 125
Leu Gly Leu Val Glu Glu Gly Ala Lys Thr Ala Pro Gly Lys Lys Arg
130 135 140
Pro Val Glu Pro Ser Pro Gln Arg Ser Pro Asp Ser Ser Thr Gly Ile
145 150 155 160
Gly Lys Lys Gly Gln Gln Pro Ala Arg Lys Arg Leu Asn Phe Gly Gln
165 170 175
Thr Gly Asp Ser Glu Ser Val Pro Asp Pro Gln Pro Ile Gly Glu Pro
180 185 190
Pro Ala Ala Pro Ser Ser Val Gly Ser Gly Thr Met Ala Ala Gly Gly
195 200 205
Gly Ala Pro Met Ala Asp Asn Asn Glu Gly Ala Asp Gly Val Gly Ser
210 215 220
Ser Ser Gly Asn Trp His Cys Asp Ser Thr Trp Leu Gly Asp Arg Val
225 230 235 240
Ile Thr Thr Ser Thr Arg Thr Trp Ala Leu Pro Thr Tyr Asn Asn His
245 250 255
Leu Tyr Lys Gln Ile Ser Asn Gly Thr Ser Gly Gly Ser Thr Asn Asp
260 265 270
Asn Thr Tyr Phe Gly Tyr Ser Thr Pro Trp Gly Tyr Phe Asp Phe Asn
275 280 285
Arg Phe His Cys His Phe Ser Pro Arg Asp Trp Gln Arg Leu Ile Asn
290 295 300
Asn Asn Trp Gly Phe Arg Pro Lys Arg Leu Ser Phe Lys Leu Phe Asn
305 310 315 320
Ile Gln Val Lys Glu Val Thr Gln Asn Glu Gly Thr Lys Thr Ile Ala
325 330 335
Asn Asn Leu Thr Ser Thr Val Gln Val Phe Ser Asp Ser Glu Tyr Gln
340 345 350
Leu Pro Tyr Val Leu Gly Ser Ala His Gln Gly Cys Leu Pro Pro Phe
355 360 365
Pro Ala Asp Val Phe Met Ile Pro Gln Tyr Gly Tyr Leu Thr Leu Asn
370 375 380
Asn Gly Ser Gln Ser Val Gly Arg Ser Ser Phe Tyr Cys Leu Glu Tyr
385 390 395 400
Phe Pro Ser Gln Met Leu Arg Thr Gly Asn Asn Phe Thr Phe Ser Tyr
405 410 415
Thr Phe Glu Asp Val Pro Phe His Ser Ser Tyr Ala His Ser Gln Ser
420 425 430
Leu Asp Arg Leu Met Asn Pro Leu Ile Asp Gln Tyr Leu Tyr Tyr Leu
435 440 445
Ala Arg Thr Gln Ser Asn Ala Gly Gly Thr Ala Gly Asn Arg Glu Leu
450 455 460
Gln Phe Tyr Gln Gly Gly Pro Thr Thr Met Ala Glu Gln Ala Lys Asn
465 470 475 480
Trp Leu Pro Gly Pro Cys Phe Arg Gln Gln Arg Val Ser Lys Thr Leu
485 490 495
Asp Gln Asn Asn Asn Ser Asn Phe Ala Trp Thr Gly Ala Thr Lys Tyr
500 505 510
His Leu Asn Gly Arg Asn Ser Leu Val Asn Pro Gly Val Ala Met Ala
515 520 525
Thr His Lys Asp Asp Glu Glu Arg Phe Phe Pro Ser Ser Gly Val Leu
530 535 540
Ile Phe Gly Lys Thr Gly Ala Ala Asn Lys Thr Thr Leu Glu Asn Val
545 550 555 560
Leu Met Thr Asn Glu Glu Glu Ile Arg Pro Thr Asn Pro Val Ala Thr
565 570 575
Glu Glu Tyr Gly Thr Val Ser Ser Asn Leu Gln Ala Ala Asn Thr Ala
580 585 590
Ala Gln Thr Gln Val Val Asn Asn Gln Gly Ala Leu Pro Gly Met Val
595 600 605
Trp Gln Asn Arg Asp Val Tyr Leu Gln Gly Pro Ile Trp Ala Lys Ile
610 615 620
Pro His Thr Asp Gly Asn Phe His Pro Ser Pro Leu Met Gly Gly Phe
625 630 635 640
Gly Leu Lys His Pro Pro Pro Gln Ile Leu Ile Lys Asn Thr Pro Val
645 650 655
Pro Ala Asn Pro Pro Glu Val Phe Thr Pro Ala Lys Phe Ala Ser Phe
660 665 670
Ile Thr Gln Tyr Ser Thr Gly Gln Val Ser Val Glu Ile Glu Trp Glu
675 680 685
Leu Gln Lys Glu Asn Ser Lys Arg Trp Asn Pro Glu Ile Gln Tyr Thr
690 695 700
Ser Asn Phe Asp Lys Gln Thr Gly Val Asp Phe Ala Val Asp Ser Gln
705 710 715 720
Gly Val Tyr Ser Glu Pro Arg Pro Ile Gly Thr Arg Tyr Leu Thr Arg
725 730 735
Asn Leu
<210>119
<211>737
<212>PRT
<213>vp1,克隆rh.69
<400>119
Met Ala Ala Asp Gly Tyr Leu Pro Asp Trp Leu Glu Asp Asn Leu Ser
1 5 10 15
Glu Gly Ile Arg Glu Trp Trp Asp Leu Lys Pro Gly Ala Pro Lys Pro
20 25 30
Lys Ala Asn Gln Gln Lys Gln Asp Asp Gly Arg Gly Leu Val Leu Pro
35 40 45
Gly Tyr Lys Tyr Leu Gly Pro Phe Asn Gly Leu Asp Lys Gly Glu Pro
50 55 60
Val Asn Ala Ala Asp Ala Ala Ala Leu Glu His Asp Lys Ala Tyr Asp
65 70 75 80
Gln Gln Leu Lys Ala Gly Asp Asn Pro Tyr Leu Arg Tyr Asn His Ala
85 90 95
Asp Ala Glu Phe Gln Glu Arg Leu Gln Glu Asp Thr Ser Phe Gly Gly
100 105 110
Asn Leu Gly Arg Ala Val Phe Gln Ala Lys Lys Arg Val Leu Glu Pro
115 120 125
Leu Gly Leu Val Glu Glu Ala Ala Lys Thr Ala Pro Gly Lys Lys Arg
130 135 140
Pro Val Glu Pro Ser Pro Gln Arg Ser Pro Asp Ser Ser Thr Gly Ile
145 150 155 160
Gly Lys Lys Gly Gln Gln Pro Ala Arg Lys Arg Leu Asn Phe Gly Gln
165 170 175
Thr Gly Asp Ser Glu Ser Val Pro Asp Pro Gln Pro Ile Gly Glu Pro
180 185 190
Pro Ala Gly Pro Ser Gly Leu Gly Ser Gly Thr Met Ala Ala Gly Gly
195 200 205
Gly Ala Pro Met Ala Asp Asn Asn Glu Gly Ala Asp Gly Val Gly Asn
210 215 220
Ala Ser Gly Asn Trp His Cys Asp Ser Thr Trp Leu Gly Asp Arg Val
225 230 235 240
Ile Thr Thr Ser Thr Arg Thr Trp Ala Leu Pro Thr Tyr Asn Asn His
245 250 255
Leu Tyr Lys Gln Ile Ser Ser Gln Ser Ala Gly Ser Thr Asn Asp Asn
260 265 270
Val Tyr Phe Gly Tyr Ser Thr Pro Trp Gly Tyr Phe Asp Phe Asn Arg
275 280 285
Phe His Cys His Phe Ser Pro Arg Asp Trp Gln Arg Leu Ile Asn Asn
290 295 300
Asn Trp Gly Phe Arg Pro Lys Lys Leu Asn Phe Lys Leu Phe Asn Ile
305 310 315 320
Gln Val Lys Glu Val Thr Thr Asn Asp Gly Val Thr Thr Ile Ala Asn
325 330 335
Asn Leu Thr Ser Thr Val Gln Val Phe Ser Asp Ser Glu Tyr Gln Leu
340 345 350
Pro Tyr Val Leu Gly Ser Ala His Gln Gly Cys Leu Pro Pro Phe Pro
355 360 365
Ala Asp Val Phe Met Ile Pro Gln Tyr Gly Tyr Leu Thr Leu Asn Asn
370 375 380
Gly Ser Gln Ser Val Gly Arg Ser Ser Phe Tyr Cys Leu Glu Tyr Phe
385 390 395 400
Pro Ser Gln Met Leu Arg Thr Gly Asn Asn Phe Thr Ile Ser Tyr Thr
405 410 415
Phe Glu Asp Val Pro Phe His Ser Ser Tyr Ala His Ser Gln Ser Leu
420 425 430
Asp Arg Leu Met Asn Pro Leu Ile Asp Gln Tyr Leu Tyr Tyr Leu Ala
435 440 445
Arg Thr Gln Ser Asn Ala Gly Gly Thr Ala Gly Asn Gln Glu Leu Gln
450 455 460
Phe Tyr Gln Gly Gly Pro Thr Thr Met Ala Glu Gln Ala Lys Asn Trp
465 470 475 480
Leu Pro Gly Pro Cys Phe Arg Gln Gln Arg Val Ser Lys Thr Leu Asp
485 490 495
Gln Asn Asn Asn Ser Asn Phe Ala Trp Thr Gly Ala Thr Lys Tyr His
500 505 510
Leu Asn Gly Arg Asn Ser Leu Val Asn Pro Gly Val Ala Met Ala Thr
515 520 525
His Lys Asp Asp Glu Glu Arg Phe Phe Pro Ser Ser Gly Val Leu Ile
530 535 540
Phe Gly Lys Thr Gly Ala Ala Asn Lys Thr Thr Leu Glu Asn Val Leu
545 550 555 560
Met Thr Asn Glu Glu Glu Ile Arg Pro Thr Asn Pro Val Ala Thr Glu
565 570 575
Glu Tyr Gly Thr Val Ser Ser Asn Leu Gln Ala Ala Asn Thr Ala Ala
580 585 590
Gln Thr Gln Val Val Asn Asn Gln Gly Ala Leu Pro Gly Met Val Trp
595 600 605
Gln Asn Arg Asp Val Tyr Leu Gln Gly Pro Ile Trp Ala Lys Ile Pro
610 615 620
His Thr Asp Gly Asn Phe His Pro Ser Pro Leu Met Gly Gly Phe Gly
625 630 635 640
Leu Lys His Pro Pro Pro Gln Ile Leu Ile Lys Asn Thr Pro Val Pro
645 650 655
Ala Asp Pro Pro Thr Ala Phe Asn Gln Ala Lys Leu Asn Ser Phe Ile
660 665 670
Thr Gln Tyr Ser Thr Gly Gln Val Ser Val Glu Ile Glu Trp Glu Leu
675 680 685
Gln Lys Glu Asn Ser Lys Arg Trp Asn Pro Glu Ile Gln Tyr Thr Ser
690 695 700
Asn Tyr Tyr Lys Ser Thr Asn Val Asp Phe Ala Val Asn Thr Glu Gly
705 710 715 720
Val Tyr Ser Glu Pro Arg Pro Ile Gly Thr Arg Tyr Leu Thr Arg Asn
725 730 735
Leu
<210>120
<211>735
<212>PRT
<213>vp1,克隆rh.60
<400>120
Met Ala Ala Asp Gly Tyr Leu Pro Asp Trp Leu Glu Asp Asn Leu Ser
1 5 10 15
Glu Gly Ile His Glu Trp Trp Asp Pro Lys Pro Gly Ala Pro Lys Pro
20 25 30
Lys Ala Asn Gln Gln Lys Gln Asp Asp Gly Arg Gly Leu Val Leu Pro
35 40 45
Gly Tyr Lys Tyr Leu Gly Pro Phe Asn Gly Leu Asp Lys Gly Glu Pro
50 55 60
Val Asn Ala Ala Asp Ala Ala Ala Leu Glu His Asp Lys Ala Tyr Asp
65 70 75 80
Gln Gln Leu Lys Ala Gly Asp Asn Pro Tyr Leu Arg Tyr Asn His Ala
85 90 95
Asp Ala Glu Phe Gln Glu Arg Leu Gln Glu Asp Thr Ser Phe Gly Gly
100 105 110
Asn Leu Gly Arg Ala Val Phe Gln Ala Lys Lys Arg Val Leu Glu Pro
115 120 125
Leu Gly Leu Val Glu Glu Gly Ala Lys Thr Ala Pro Gly Lys Lys Arg
130 135 140
Pro Val Glu Pro Ser Pro Gln Arg Ser Pro Asp Ser Ser Thr Gly Ile
145 150 155 160
Gly Lys Lys Gly Gln Gln Pro Ala Arg Lys Arg Leu Asn Phe Gly Gln
165 170 175
Thr Gly Asp Ser Glu Ser Val Pro Asp Pro Gln Pro Ile Gly Glu Pro
180 185 190
Pro Ala Ala Pro Ser Ser Val Gly Ser Gly Thr Met Ala Ala Gly Gly
195 200 205
Gly Ala Pro Met Ala Asp Asn Asn Glu Gly Ala Asp Gly Val Gly Ser
210 215 220
Ser Ser Gly Asn Trp His Cys Asp Ser Thr Trp Leu Gly Asp Arg Val
225 230 235 240
Ile Thr Thr Ser Thr Arg Thr Trp Ala Leu Pro Thr His Asn Asn His
245 250 255
Leu Tyr Lys Gln Ile Ser Asn Gly Thr Ser Gly Gly Ser Thr Asn Asp
260 265 270
Asn Val Tyr Phe Gly Tyr Ser Thr Pro Trp Gly Tyr Phe Asp Phe Asn
275 280 285
Arg Phe His Cys His Phe Ser Pro Arg Asp Arg Gln Arg Leu Ile Asn
290 295 300
Asn Asn Trp Gly Phe Arg Pro Lys Arg Leu Ser Phe Lys Leu Phe Asn
305 310 315 320
Ile Gln Val Lys Glu Val Thr Gln Asn Glu Gly Thr Lys Thr Ile Ala
325 330 335
Asn Asn Leu Thr Ser Thr Ile Gln Val Phe Thr Asp Ser Glu Tyr Gln
340 345 350
Leu Pro Tyr Val Leu Gly Ser Ala His Gln Gly Cys Leu Pro Pro Phe
355 360 365
Pro Ala Asp Val Phe Met Ile Pro Gln Tyr Gly Tyr Leu Thr Leu Asn
370 375 380
Asn Gly Ser Gln Ala Val Gly Arg Ser Ser Phe Tyr Cys Leu Glu Tyr
385 390 395 400
Phe Pro Ser Gln Met Leu Arg Thr Gly Asn Asn Phe Ser Phe Ser Tyr
405 410 415
Thr Phe Glu Asp Val Pro Phe His Ser Ser Tyr Ala His Ser Gln Ser
420 425 430
Leu Asp Arg Leu Met Asn Pro Leu Ile Asp Gln Tyr Leu Tyr Tyr Leu
435 440 445
Ser Arg Thr Gln Ser Thr Glu Gly Thr Ala Gly Thr Gln Gln Leu Leu
450 455 460
Phe Ser Gln Ala Gly Pro Ser Asn Met Ser Ala Gln Ala Arg Asn Trp
465 470 475 480
Leu Pro Gly Pro Cys Tyr Arg Gln Gln Arg Val Ser Thr Thr Leu Ser
485 490 495
Gln Asn Asn Asn Ser Asn Phe Ala Trp Thr Gly Ala Thr Lys Tyr His
500 505 510
Leu Asn Gly Arg Asp Ser Leu Val Asn Pro Gly Val Ala Met Ala Thr
515 520 525
His Lys Asp Asp Glu Glu Arg Phe Phe Pro Ser Ser Gly Val Leu Ile
530 535 540
Phe Gly Lys Thr Gly Ala Ala Asn Lys Thr Thr Leu Glu Asn Val Leu
545 550 555 560
Met Thr Asn Glu Glu Glu Ile Arg Pro Thr Asn Pro Val Ala Thr Glu
565 570 575
Glu Tyr Gly Thr Val Ser Ser Asn Leu Gln Ala Ala Asn Thr Ala Ala
580 585 590
Gln Thr Gln Val Val Asn Asn Gln Gly Ala Leu Pro Gly Met Val Trp
595 600 605
Gln Asn Arg Asp Val Tyr Leu Gln Gly Pro Ile Trp Ala Lys Ile Pro
610 615 620
His Thr Asp Gly Asn Phe His Pro Ser Pro Leu Met Gly Gly Phe Gly
625 630 635 640
Leu Lys His Pro Gln Ile Leu Ile Lys Asn Thr Pro Val Pro Ala Asn
645 650 655
Pro Pro Glu Val Phe Thr Pro Ala Lys Phe Ala Ser Phe Ile Thr Gln
660 665 670
Tyr Ser Thr Gly Gln Val Ser Val Glu Ile Glu Trp Glu Leu Gln Lys
675 680 685
Glu Asn Ser Lys Arg Trp Asn Pro Glu Ile Gln Tyr Thr Ser Asn Phe
690 695 700
Asp Lys Gln Thr Gly Val Asp Phe Ala Val Asp Ser Gln Gly Val Tyr
705 710 715 720
Ser Glu Pro Arg Pro Ile Gly Thr Arg Tyr Leu Thr Arg Asn Leu
725 730 735
<210>121
<211>736
<212>PRT
<213>vp1,克隆hu.31
<400>121
Met Ala Ala Asp Gly Tyr Leu Pro Asp Trp Leu Glu Asp Thr Leu Ser
1 5 10 15
Glu Gly Ile Arg Gln Trp Trp Lys Leu Lys Pro Gly Pro Pro Pro Pro
20 25 30
Lys Pro Ala Glu Arg His Lys Asp Asp Ser Arg Gly Leu Val Leu Pro
35 40 45
Gly Tyr Lys Tyr Leu Gly Pro Gly Asn Gly Leu Asp Lys Gly Glu Pro
50 55 60
Val Asn Ala Ala Asp Ala Ala Ala Leu Glu His Asp Lys Ala Tyr Asp
65 70 75 80
Gln Gln Leu Lys Ala Gly Asp Asn Pro Tyr Leu Lys Tyr Asn His Ala
85 90 95
Asp Ala Glu Phe Gln Glu Arg Leu Lys Glu Asp Thr Ser Phe Gly Gly
100 105 110
Asn Leu Gly Arg Ala Val Phe Gln Ala Lys Lys Arg Leu Leu Glu Pro
115 120 125
Leu Gly Leu Val Glu Glu Ala Ala Lys Thr Ala Pro Gly Lys Lys Arg
130 135 140
Pro Val Glu Gln Ser Pro Gln Glu Pro Asp Ser Ser Ala Gly Ile Gly
145 150 155 160
Lys Ser Gly Ser Gln Pro Ala Lys Lys Lys Leu Asn Phe Gly Gln Thr
165 170 175
Gly Asp Thr Glu Ser Val Pro Asp Pro Gln Pro Ile Gly Glu Pro Pro
180 185 190
Ala Ala Pro Ser Gly Val Gly Ser Leu Thr Met Ala Ser Gly Gly Gly
195 200 205
Ala Pro Val Ala Asp Asn Asn Glu Gly Ala Asp Gly Val Gly Ser Ser
210 215 220
Ser Gly Asn Trp His Cys Asp Ser Gln Trp Leu Gly Asp Arg Val Ile
225 230 235 240
Thr Thr Ser Thr Arg Thr Trp Ala Leu Pro Thr Tyr Asn Asn His Leu
245 250 255
Tyr Lys Gln Ile Ser Asn Ser Thr Ser Gly Gly Ser Ser Asn Asp Asn
260 265 270
Ala Tyr Phe Gly Tyr Ser Thr Pro Trp Gly Tyr Phe Asp Phe Asn Arg
275 280 285
Phe His Cys His Phe Ser Pro Arg Asp Trp Gln Arg Leu Ile Asn Asn
290 295 300
Asn Trp Gly Phe Arg Pro Lys Arg Leu Asn Phe Lys Leu Phe Asn Ile
305 310 315 320
Gln Val Lys Glu Val Thr Asp Asn Asn Gly Val Lys Thr Ile Ala Asn
325 330 335
Asn Leu Thr Ser Thr Val Gln Val Phe Thr Asp Ser Asp Tyr Gln Leu
340 345 350
Pro Tyr Val Leu Gly Ser Ala His Glu Gly Cys Leu Pro Pro Phe Pro
355 360 365
Ala Asp Val Phe Met Ile Pro Gln Tyr Gly Tyr Leu Thr Leu Asn Asp
370 375 380
Gly Gly Gln Ala Val Gly Arg Ser Ser Phe Tyr Cys Leu Glu Tyr Phe
385 390 395 400
Pro Ser Gln Met Leu Arg Thr Gly Asn Asn Phe Gln Phe Ser Tyr Glu
405 410 415
Phe Glu Asn Val Pro Phe His Ser Ser Tyr Ala His Ser Gln Ser Leu
420 425 430
Asp Arg Leu Met Asn Pro Leu Ile Asp Gln Tyr Leu Tyr Tyr Leu Ser
435 440 445
Lys Thr Ile Asn Gly Ser Gly Gln Asn Gln Gln Thr Leu Lys Phe Ser
450 455 460
Val Ala Gly Pro Ser Asn Met Ala Val Gln Gly Arg Asn Tyr Ile Pro
465 470 475 480
Gly Pro Ser Tyr Arg Gln Gln Arg Val Ser Thr Thr Val Thr Gln Asn
485 490 495
Asn Asn Ser Glu Phe Ala Trp Pro Gly Ala Ser Ser Trp Ala Leu Asn
500 505 510
Gly Arg Asn Ser Leu Met Asn Pro Gly Pro Ala Met Ala Ser His Lys
515 520 525
Glu Gly Glu Asp Arg Phe Phe Pro Leu Ser Gly Ser Leu Ile Phe Gly
530 535 540
Lys Gln Gly Thr Gly Arg Asp Asn Val Asp Ala Asp Lys Val Met Ile
545 550 555 560
Thr Asn Glu Glu Glu Ile Lys Thr Thr Asn Pro Val Ala Thr Glu Ser
565 570 575
Tyr Gly Gln Val Ala Thr Asn His Gln Ser Ala Gln Ala Gln Ala Gln
580 585 590
Thr Gly Trp Val Gln Asn Gln Gly Ile Leu Pro Gly Met Val Trp Gln
595 600 605
Asp Arg Asp Val Tyr Leu Gln Gly Pro Ile Trp Ala Lys Ile Pro His
610 615 620
Thr Asp Gly Asn Phe His Pro Ser Pro Leu Met Gly Gly Phe Gly Met
625 630 635 640
Lys His Pro Pro Pro Gln Ile Leu Ile Lys Asn Thr Pro Val Pro Ala
645 650 655
Asp Pro Pro Thr Ala Phe Asn Lys Asp Lys Leu Asn Ser Phe Ile Thr
660 665 670
Gln Tyr Ser Thr Gly Gln Val Ser Val Glu Ile Glu Trp Glu Leu Gln
675 680 685
Lys Glu Asn Ser Lys Arg Trp Asn Pro Glu Ile Gln Tyr Thr Ser Asn
690 695 700
Tyr Tyr Lys Ser Asn Asn Val Glu Phe Ala Val Ser Thr Glu Gly Val
705 710 715 720
Tyr Ser Glu Pro Arg Pro Ile Gly Thr Arg Tyr Leu Thr Arg Asn Leu
725 730 735
<210>122
<211>736
<212>PRT
<213>vp1,克隆hu.32
<400>122
Met Ala Ala Asp Gly Tyr Leu Pro Asp Trp Leu Glu Asp Thr Leu Ser
1 5 10 15
Glu Gly Ile Arg Gln Trp Trp Lys Leu Lys Pro Gly Pro Pro Pro Pro
20 25 30
Lys Pro Ala Glu Arg His Lys Asp Asp Ser Arg Gly Leu Val Leu Pro
35 40 45
Gly Tyr Lys Tyr Leu Gly Pro Gly Asn Gly Leu Asp Lys Gly Glu Pro
50 55 60
Val Asn Ala Ala Asp Ala Ala Ala Leu Glu His Asp Lys Ala Tyr Asp
65 70 75 80
Gln Gln Leu Lys Ala Gly Asp Asn Pro Tyr Leu Lys Tyr Asn His Ala
85 90 95
Asp Ala Glu Phe Gln Glu Arg Leu Lys Glu Asp Thr Ser Phe Gly Gly
100 105 110
Asn Leu Gly Arg Ala Val Phe Gln Ala Lys Lys Arg Leu Leu Glu Pro
115 120 125
Leu Gly Leu Val Glu Glu Ala Ala Lys Thr Ala Pro Gly Lys Lys Arg
130 135 140
Pro Val Glu Gln Ser Pro Gln Glu Pro Asp Ser Ser Ala Gly Ile Gly
145 150 155 160
Lys Ser Gly Ser Gln Pro Ala Lys Lys Lys Leu Asn Phe Gly Gln Thr
165 170 175
Gly Asp Thr Glu Ser Val Pro Asp Pro Gln Pro Ile Gly Glu Pro Pro
180 185 190
Ala Ala Pro Ser Gly Val Gly Ser Leu Thr Met Ala Ser Gly Gly Gly
195 200 205
Ala Pro Val Ala Asp Asn Asn Glu Gly Ala Asp Gly Val Gly Ser Ser
210 215 220
Ser Gly Asn Trp His Cys Asp Ser Gln Trp Leu Gly Asp Arg Val Ile
225 230 235 240
Thr Thr Ser Thr Arg Thr Trp Ala Leu Pro Thr Tyr Asn Asn His Leu
245 250 255
Tyr Lys Gln Ile Ser Asn Ser Thr Ser Gly Gly Ser Ser Asn Asp Asn
260 265 270
Ala Tyr Phe Gly Tyr Ser Thr Pro Trp Gly Tyr Phe Asp Phe Asn Arg
275 280 285
Phe His Cys His Phe Ser Pro Arg Asp Trp Gln Arg Leu Ile Asn Asn
290 295 300
Asn Trp Gly Phe Arg Pro Lys Arg Leu Asn Phe Lys Leu Phe Asn Ile
305 310 315 320
Gln Val Lys Glu Val Thr Asp Asn Asn Gly Val Lys Thr Ile Ala Asn
325 330 335
Asn Leu Thr Ser Thr Val Gln Val Phe Thr Asp Ser Asp Tyr Gln Leu
340 345 350
Pro Tyr Val Leu Gly Ser Ala His Glu Gly Cys Leu Pro Pro Phe Pro
355 360 365
Ala Asp Val Phe Met Ile Pro Gln Tyr Gly Tyr Leu Thr Leu Asn Asp
370 375 380
Gly Ser Gln Ala Val Gly Arg Ser Ser Phe Tyr Cys Leu Glu Tyr Phe
385 390 395 400
Pro Ser Gln Met Leu Arg Thr Gly Asn Asn Phe Gln Phe Ser Tyr Glu
405 410 415
Phe Glu Asn Val Pro Phe His Ser Ser Tyr Ala His Ser Gln Ser Leu
420 425 430
Asp Arg Leu Met Asn Pro Leu Ile Asp Gln Tyr Leu Tyr Tyr Leu Ser
435 440 445
Lys Thr Ile Asn Gly Ser Gly Gln Asn Gln Gln Thr Leu Lys Phe Ser
450 455 460
Val Ala Gly Pro Ser Asn Met Ala Val Gln Gly Arg Asn Tyr Ile Pro
465 470 475 480
Gly Pro Ser Tyr Arg Gln Gln Arg Val Ser Thr Thr Val Thr Gln Asn
485 490 495
Asn Asn Ser Glu Phe Ala Trp Pro Gly Ala Ser Ser Trp Ala Leu Asn
500 505 510
Gly Arg Asn Ser Leu Met Asn Pro Gly Pro Ala Met Ala Ser His Lys
515 520 525
Glu Gly Glu Asp Arg Phe Phe Pro Leu Ser Gly Ser Leu Ile Phe Gly
530 535 540
Lys Gln Gly Thr Gly Arg Asp Asn Val Asp Ala Asp Lys Val Met Ile
545 550 555 560
Thr Asn Glu Glu Glu Ile Lys Thr Thr Asn Pro Val Ala Thr Glu Ser
565 570 575
Tyr Gly Gln Val Ala Thr Asn His Gln Ser Ala Gln Ala Gln Ala Gln
580 585 590
Thr Gly Trp Val Gln Asn Gln Gly Ile Leu Pro Gly Met Val Trp Gln
595 600 605
Asp Arg Asp Val Tyr Leu Gln Gly Pro Ile Trp Ala Lys Ile Pro His
610 615 620
Thr Asp Gly Asn Phe His Pro Ser Pro Leu Met Gly Gly Phe Gly Met
625 630 635 640
Lys His Pro Pro Pro Gln Ile Leu Ile Lys Asn Thr Pro Val Pro Ala
645 650 655
Asp Pro Pro Thr Ala Phe Asn Lys Asp Lys Leu Asn Ser Phe Ile Thr
660 665 670
Gln Tyr Ser Thr Gly Gln Val Ser Val Glu Ile Glu Trp Glu Leu Gln
675 680 685
Lys Glu Asn Ser Lys Arg Trp Asn Pro Glu Ile Gln Tyr Thr Ser Asn
690 695 700
Tyr Tyr Lys Ser Asn Asn Val Glu Phe Ala Val Asn Thr Glu Gly Val
705 710 715 720
Tyr Ser Glu Pro Arg Pro Ile Gly Thr Arg Tyr Leu Thr Arg Asn Leu
725 730 735
<210>123
<211>736
<212>PRT
<213>hu.14\AAV9的衣壳
<400>123
Met Ala Ala Asp Gly Tyr Leu Pro Asp Trp Leu Glu Asp Asn Leu Ser
1 5 10 15
Glu Gly Ile Arg Glu Trp Trp Ala Leu Lys Pro Gly Ala Pro Gln Pro
20 25 30
Lys Ala Asn Gln Gln His Gln Asp Asn Ala Arg Gly Leu Val Leu Pro
35 40 45
Gly Tyr Lys Tyr Leu Gly Pro Gly Asn Gly Leu Asp Lys Gly Glu Pro
50 55 60
Val Asn Ala Ala Asp Ala Ala Ala Leu Glu His Asp Lys Ala Tyr Asp
65 70 75 80
Gln Gln Leu Lys Ala Gly Asp Asn Pro Tyr Leu Lys Tyr Asn His Ala
85 90 95
Asp Ala Glu Phe Gln Glu Arg Leu Lys Glu Asp Thr Ser Phe Gly Gly
100 105 110
Asn Leu Gly Arg Ala Val Phe Gln Ala Lys Lys Arg Leu Leu Glu Pro
115 120 125
Leu Gly Leu Val Glu Glu Ala Ala Lys Thr Ala Pro Gly Lys Lys Arg
130 135 140
Pro Val Glu Gln Ser Pro Gln Glu Pro Asp Ser Ser Ala Gly Ile Gly
145 150 155 160
Lys Ser Gly Ala Gln Pro Ala Lys Lys Arg Leu Asn Phe Gly Gln Thr
165 170 175
Gly Asp Thr Glu Ser Val Pro Asp Pro Gln Pro Ile Gly Glu Pro Pro
180 185 190
Ala Ala Pro Ser Gly Val Gly Ser Leu Thr Met Ala Ser Gly Gly Gly
195 200 205
Ala Pro Val Ala Asp Asn Asn Glu Gly Ala Asp Gly Val Gly Ser Ser
210 215 220
Ser Gly Asn Trp His Cys Asp Ser Gln Trp Leu Gly Asp Arg Val Ile
225 230 235 240
Thr Thr Ser Thr Arg Thr Trp Ala Leu Pro Thr Tyr Asn Asn His Leu
245 250 255
Tyr Lys Gln Ile Ser Asn Ser Thr Ser Gly Gly Ser Ser Asn Asp Asn
260 265 270
Ala Tyr Phe Gly Tyr Ser Thr Pro Trp Gly Tyr Phe Asp Phe Asn Arg
275 280 285
Phe His Cys His Phe Ser Pro Arg Asp Trp Gln Arg Leu Ile Asn Asn
290 295 300
Asn Trp Gly Phe Arg Pro Lys Arg Leu Asn Phe Lys Leu Phe Asn Ile
305 310 315 320
Gln Val Lys Glu Val Thr Asp Asn Asn Gly Val Lys Thr Ile Ala Asn
325 330 335
Asn Leu Thr Ser Thr Val Gln Val Phe Thr Asp Ser Asp Tyr Gln Leu
340 345 350
Pro Tyr Val Leu Gly Ser Ala His Glu Gly Cys Leu Pro Pro Phe Pro
355 360 365
Ala Asp Val Phe Met Ile Pro Gln Tyr Gly Tyr Leu Thr Leu Asn Asp
370 375 380
Gly Ser Gln Ala Val Gly Arg Ser Ser Phe Tyr Cys Leu Glu Tyr Phe
385 390 395 400
Pro Ser Gln Met Leu Arg Thr Gly Asn Asn Phe Gln Phe Ser Tyr Glu
405 410 415
Phe Glu Asn Val Pro Phe His Ser Ser Tyr Ala His Ser Gln Ser Leu
420 425 430
Asp Arg Leu Met Asn Pro Leu Ile Asp Gln Tyr Leu Tyr Tyr Leu Ser
435 440 445
Lys Thr Ile Asn Gly Ser Gly Gln Asn Gln Gln Thr Leu Lys Phe Ser
450 455 460
Val Ala Gly Pro Ser Asn Met Ala Val Gln Gly Arg Asn Tyr Ile Pro
465 470 475 480
Gly Pro Ser Tyr Arg Gln Gln Arg Val Ser Thr Thr Val Thr Gln Asn
485 490 495
Asn Asn Ser Glu Phe Ala Trp Pro Gly Ala Ser Ser Trp Ala Leu Asn
500 505 510
Gly Arg Asn Ser Leu Met Asn Pro Gly Pro Ala Met Ala Ser His Lys
515 520 525
Glu Gly Glu Asp Arg Phe Phe Pro Leu Ser Gly Ser Leu Ile Phe Gly
530 535 540
Lys Gln Gly Thr Gly Arg Asp Asn Val Asp Ala Asp Lys Val Met Ile
545 550 555 560
Thr Asn Glu Glu Glu Ile Lys Thr Thr Asn Pro Val Ala Thr Glu Ser
565 570 575
Tyr Gly Gln Val Ala Thr Asn His Gln Ser Ala Gln Ala Gln Ala Gln
580 585 590
Thr Gly Trp Val Gln Asn Gln Gly Ile Leu Pro Gly Met Val Trp Gln
595 600 605
Asp Arg Asp Val Tyr Leu Gln Gly Pro Ile Trp Ala Lys Ile Pro His
610 615 620
Thr Asp Gly Asn Phe His Pro Ser Pro Leu Met Gly Gly Phe Gly Met
625 630 635 640
Lys His Pro Pro Pro Gln Ile Leu Ile Lys Asn Thr Pro Val Pro Ala
645 650 655
Asp Pro Pro Thr Ala Phe Asn Lys Asp Lys Leu Asn Ser Phe Ile Thr
660 665 670
Gln Tyr Ser Thr Gly Gln Val Ser Val Glu Ile Glu Trp Glu Leu Gln
675 680 685
Lys Glu Asn Ser Lys Arg Trp Asn Pro Glu Ile Gln Tyr Thr Ser Asn
690 695 700
Tyr Tyr Lys Ser Asn Asn Val Glu Phe Ala Val Asn Thr Glu Gly Val
705 710 715 720
Tyr Ser Glu Pro Arg Pro Ile Gly Thr Arg Tyr Leu Thr Arg Asn Leu
725 730 735
<210>124
<211>735
<212>PRT
<213>vp1,克隆hu.33
<400>124
Met Ala Ala Asp Gly Tyr Leu Pro Asp Trp Leu Glu Asp Thr Leu Ser
1 5 10 15
Glu Gly Ile Arg Gln Arg Trp Lys Leu Lys Pro Gly Pro Pro Pro Pro
20 25 30
Glu Pro Ala Glu Arg His Lys Asp Asp Ser Arg Gly Leu Val Leu Pro
35 40 45
Gly Tyr Lys Tyr Leu Gly Pro Phe Asn Gly Leu Asp Lys Gly Glu Pro
50 55 60
Val Asn Glu Ala Asp Ala Ala Ala Leu Glu His Asp Lys Ala Tyr Asp
65 70 75 80
Arg Gln Leu Asp Ser Gly Asp Asn Pro Tyr Leu Lys Tyr Asn His Ala
85 90 95
Asp Ala Glu Phe Gln Glu Arg Leu Lys Glu Asp Thr Ser Phe Gly Gly
100 105 110
Asn Leu Gly Arg Ala Val Phe Gln Ala Lys Lys Arg Val Leu Glu Pro
115 120 125
Leu Gly Leu Val Glu Glu Pro Val Lys Thr Ala Pro Gly Lys Lys Arg
130 135 140
Pro Val Glu His Ser Pro Val Glu Pro Asp Ser Ser Ser Gly Thr Gly
145 150 155 160
Lys Ala Gly Gln Gln Pro Ala Arg Lys Arg Leu Asn Phe Gly Gln Thr
165 170 175
Gly Asp Ala Asp Ser Val Pro Asp Pro Gln Pro Leu Gly Gln Pro Pro
180 185 190
Ala Ala Pro Ser Gly Leu Gly Thr Asn Thr Met Ala Thr Gly Ser Gly
195 200 205
Ala Pro Met Ala Asp Asn Asn Glu Gly Ala Asp Gly Val Gly Asn Ser
210 215 220
Ser Gly Asn Trp His Cys Asp Ser Thr Trp Met Gly Asp Arg Val Ile
225 230 235 240
Thr Thr Ser Thr Arg Thr Trp Ala Leu Pro Thr Tyr Asn Asn His Leu
245 250 255
Tyr Lys Gln Ile Ser Ser Gln Ser Gly Ala Ser Asn Asp Asn His Tyr
260 265 270
Phe Gly Tyr Ser Thr Pro Trp Gly Tyr Phe Asp Phe Asn Arg Phe His
275 280 285
Cys His Phe Ser Pro Arg Asp Trp Gln Arg Leu Ile Asn Asn Asn Trp
290 295 300
Gly Phe Arg Pro Lys Arg Leu Asn Phe Lys Leu Phe Asn Ile Gln Val
305 310 315 320
Lys Glu Val Thr Gln Asn Asp Gly Thr Thr Thr Ile Ala Asn Asn Leu
325 330 335
Thr Gly Thr Val Gln Val Phe Thr Asp Ser Glu Tyr Gln Leu Pro Tyr
340 345 350
Val Leu Gly Ser Ala His Gln Gly Cys Leu Pro Pro Phe Pro Ala Asp
355 360 365
Val Phe Met Val Pro Gln Tyr Gly Tyr Leu Thr Leu Asn Asn Gly Ser
370 375 380
Gln Ala Val Gly Arg Ser Ser Phe Tyr Cys Leu Glu Tyr Phe Pro Ser
385 390 395 400
Gln Met Leu Arg Thr Gly Asn Asn Phe Thr Phe Ser Tyr Thr Phe Glu
405 410 415
Asp Val Pro Phe His Ser Ser Tyr Ala His Ser Gln Ser Leu Gly Arg
420 425 430
Leu Met Asn Pro Leu Ile Asp Gln Tyr Leu Tyr Tyr Leu Ser Arg Thr
435 440 445
Asn Thr Pro Ser Gly Thr Thr Thr Gln Ser Arg Leu Gln Phe Ser Gln
450 455 460
Ala Gly Ala Ser Asp Ile Arg Asp Gln Ser Arg Asn Trp Leu Pro Gly
465 470 475 480
Pro Cys Tyr Arg Gln Gln Arg Val Ser Lys Thr Ser Ala Asp Asn Asn
485 490 495
Asn Ser Glu Tyr Ser Trp Thr Gly Ala Thr Lys Tyr His Leu Asn Gly
500 505 510
Arg Asp Ser Leu Val Asn Pro Gly Pro Ala Met Ala Ser His Lys Asp
515 520 525
Asp Glu Glu Lys Phe Phe Pro Gln Ser Gly Val Leu Ile Phe Gly Lys
530 535 540
Gln Gly Ser Glu Lys Thr Asn Val Asp Ile Glu Lys Val Met Ile Thr
545 550 555 560
Asp Glu Glu Glu Ile Arg Thr Thr Asn Pro Val Ala Thr Glu Gln Tyr
565 570 575
Gly Ser Val Ser Thr Asn Leu Gln Arg Gly Asn Arg Gln Ala Ala Thr
580 585 590
Ala Asp Val Asn Thr Gln Gly Val Leu Pro Gly Met Val Trp Gln Asp
595 600 605
Arg Asp Val Tyr Leu Gln Gly Pro Ile Trp Ala Lys Ile Pro His Thr
610 615 620
Asp Gly His Phe His Pro Ser Pro Leu Met Gly Gly Phe Gly Leu Lys
625 630 635 640
His Pro Pro Pro Gln Ile Leu Ile Lys Asn Thr Pro Val Pro Ala Asn
645 650 655
Pro Ser Thr Thr Phe Ser Ala Ala Lys Phe Ala Ser Phe Ile Thr Gln
660 665 670
Tyr Ser Thr Gly Gln Val Ser Val Glu Ile Glu Trp Glu Leu Gln Lys
675 680 685
Glu Asn Ser Lys Arg Trp Asn Pro Glu Ile Gln Tyr Thr Ser Asn Tyr
690 695 700
Asn Lys Ser Val Asn Val Asp Phe Thr Val Asp Thr Asn Gly Val Tyr
705 710 715 720
Ser Glu Pro Arg Pro Ile Gly Thr Arg Tyr Leu Thr Arg Asn Leu
725 730 735
<210>125
<211>735
<212>PRT
<213>vp1,克隆hu.34
<400>125
Met Ala Ala Asp Gly Tyr Leu Pro Asp Trp Leu Glu Asp Thr Leu Ser
1 5 10 15
Glu Gly Ile Arg Gln Arg Trp Lys Leu Lys Pro Gly Pro Pro Pro Pro
20 25 30
Glu Pro Ala Glu Arg His Lys Asp Asp Ser Arg Gly Leu Val Leu Pro
35 40 45
Gly Tyr Lys Tyr Leu Gly Pro Phe Asn Gly Leu Asp Lys Gly Glu Pro
50 55 60
Val Asn Glu Ala Asp Ala Ala Ala Leu Glu His Asp Lys Ala Tyr Asp
65 70 75 80
Arg Gln Leu Asp Ser Gly Asp Asn Pro Tyr Leu Lys Tyr Asn His Ala
85 90 95
Asp Ala Glu Phe Gln Glu Arg Leu Lys Glu Asp Thr Ser Phe Gly Gly
100 105 110
Asn Leu Gly Arg Ala Val Phe Gln Ala Lys Lys Arg Val Leu Glu Pro
115 120 125
Leu Gly Leu Val Glu Glu Pro Val Lys Thr Ala Pro Gly Lys Lys Arg
130 135 140
Pro Val Glu His Ser Pro Val Glu Pro Asp Ser Ser Ser Gly Thr Gly
145 150 155 160
Lys Ala Gly Gln Gln Pro Ala Arg Lys Arg Leu Asn Phe Gly Gln Thr
165 170 175
Gly Asp Ala Asp Ser Val Pro Asp Pro Gln Pro Leu Gly Gln Pro Pro
180 185 190
Ala Ala Pro Ser Gly Leu Gly Thr Asn Thr Met Ala Thr Gly Ser Gly
195 200 205
Ala Pro Met Ala Asp Asn Asn Glu Gly Ala Asp Gly Val Gly Asn Ser
210 215 220
Ser Gly Asn Trp His Cys Asp Ser Thr Trp Met Gly Asp Arg Val Ile
225 230 235 240
Thr Thr Ser Thr Arg Thr Trp Ala Leu Pro Thr Tyr Asn Asn His Leu
245 250 255
Tyr Lys Gln Ile Ser Ser Gln Ser Gly Ala Ser Asn Asp Asn His Tyr
260 265 270
Phe Gly Tyr Ser Thr Pro Trp Gly Tyr Phe Asp Phe Asn Arg Phe His
275 280 285
Cys His Phe Ser Pro Arg Asp Trp Gln Arg Leu Ile Asn Asn Asn Trp
290 295 300
Gly Phe Arg Pro Lys Arg Leu Asn Phe Lys Leu Phe Asn Ile Gln Val
305 310 315 320
Lys Glu Val Thr Gln Asn Asp Gly Thr Thr Thr Ile Ala Asn Asn Leu
325 330 335
Thr Ser Thr Val Gln Val Phe Thr Asp Ser Glu Tyr Gln Leu Pro Tyr
340 345 350
Val Leu Gly Ser Ala His Gln Gly Cys Leu Pro Pro Phe Pro Ala Asp
355 360 365
Val Phe Met Val Pro Gln Tyr Gly Tyr Leu Thr Leu Asn Asn Glu Ser
370 375 380
Gln Ala Val Gly Arg Ser Ser Phe Tyr Cys Leu Glu Tyr Phe Pro Ser
385 390 395 400
Gln Met Leu Arg Thr Gly Asn Asn Phe Thr Phe Ser Tyr Thr Phe Glu
405 410 415
Asp Val Pro Phe His Ser Ser Tyr Ala His Ser Gln Ser Leu Gly Arg
420 425 430
Leu Met Asn Pro Leu Ile Asp Gln Tyr Leu Tyr Tyr Leu Ser Arg Thr
435 440 445
Asn Thr Pro Ser Gly Thr Thr Thr Gln Ser Arg Leu Gln Phe Ser Gln
450 455 460
Ala Gly Ala Ser Asp Ile Arg Asp Gln Ser Arg Asn Trp Leu Pro Gly
465 470 475 480
Pro Cys Tyr Arg Gln Gln Arg Val Ser Lys Thr Ser Ala Asp Asn Asn
485 490 495
Asn Ser Glu Tyr Ser Trp Thr Gly Ala Thr Lys Tyr His Leu Asn Gly
500 505 510
Arg Asp Ser Leu Val Asn Pro Gly Pro Ala Met Ala Ser His Lys Asp
515 520 525
Asp Glu Glu Lys Phe Phe Pro Gln Ser Gly Val Leu Ile Phe Gly Lys
530 535 540
Gln Gly Ser Glu Lys Thr Asn Val Asp Ile Glu Lys Val Met Ile Thr
545 550 555 560
Asp Glu Glu Glu Ile Arg Thr Thr Asn Pro Val Ala Thr Glu Gln Tyr
565 570 575
Gly Ser Val Ser Thr Asn Leu Gln Arg Gly Asn Arg Gln Ala Ala Thr
580 585 590
Ala Asp Val Asn Thr Gln Gly Val Leu Pro Gly Met Val Trp Gln Asp
595 600 605
Arg Asp Val Tyr Leu Gln Gly Pro Ile Trp Ala Lys Ile Pro His Thr
610 615 620
Asp Gly His Phe His Pro Ser Pro Leu Met Gly Gly Phe Gly Leu Lys
625 630 635 640
His Pro Pro Pro Gln Ile Leu Ile Lys Asn Thr Pro Val Pro Ala Asn
645 650 655
Pro Ser Thr Thr Phe Ser Ala Ala Lys Phe Ala Ser Phe Ile Thr Gln
660 665 670
Tyr Ser Thr Gly Gln Val Ser Val Glu Ile Glu Trp Glu Leu Gln Lys
675 680 685
Glu Asn Ser Lys Arg Trp Asn Pro Glu Ile Gln Tyr Thr Ser Asn Tyr
690 695 700
Asn Lys Ser Val Asn Val Asp Phe Thr Val Asp Thr Asn Gly Val Tyr
705 710 715 720
Ser Glu Pro Arg Pro Ile Gly Thr Arg Tyr Leu Thr Arg Asn Leu
725 730 735
<210>126
<211>735
<212>PRT
<213>vp1,克隆hu.36
<400>126
Met Ala Ala Asp Gly Tyr Leu Pro Asp Trp Leu Glu Asp Thr Leu Ser
1 5 10 15
Glu Gly Ile Arg Gln Arg Trp Lys Leu Lys Pro Gly Pro Pro Pro Pro
20 25 30
Glu Pro Ala Glu Arg His Lys Asp Asp Ser Arg Gly Leu Val Leu Pro
35 40 45
Gly Tyr Lys Tyr Leu Gly Pro Phe Asn Gly Leu Asp Lys Gly Glu Pro
50 55 60
Val Asn Glu Ala Asp Ala Ala Ala Leu Glu His Asp Lys Ala Tyr Asp
65 70 75 80
Arg Gln Leu Asp Ser Gly Asp Asn Pro Tyr Leu Lys Tyr Asn His Ala
85 90 95
Asp Ala Glu Phe Gln Glu Arg Leu Lys Glu Asp Thr Ser Phe Gly Gly
100 105 110
Asn Leu Gly Arg Ala Val Phe Gln Ala Lys Lys Arg Val Leu Glu Pro
115 120 125
Leu Gly Leu Val Glu Glu Pro Val Lys Thr Ala Pro Gly Lys Lys Arg
130 135 140
Pro Val Glu His Ser Pro Val Glu Pro Asp Ser Ser Ser Gly Thr Gly
145 150 155 160
Lys Ala Gly Gln Gln Pro Ala Arg Lys Arg Leu Asn Phe Gly Gln Thr
165 170 175
Gly Asp Ala Asp Ser Val Pro Asp Pro Gln Pro Leu Gly Gln Pro Pro
180 185 190
Ala Ala Pro Ser Gly Leu Gly Thr Asn Thr Met Ala Thr Gly Ser Gly
195 200 205
Ala Pro Met Ala Asp Asn Asn Glu Gly Ala Asp Gly Val Gly Asn Ser
210 215 220
Ser Gly Asn Trp His Cys Asp Ser Thr Trp Met Gly Asp Arg Val Ile
225 230 235 240
Thr Thr Ser Thr Arg Thr Trp Ala Leu Pro Thr Tyr Asn Asn His Leu
245 250 255
Tyr Lys Gln Ile Ser Ser Gln Ser Gly Ala Ser Asn Asp Asn His Tyr
260 265 270
Phe Gly Tyr Ser Thr Pro Trp Gly Tyr Phe Asp Phe Asn Arg Phe His
275 280 285
Cys His Phe Ser Pro Arg Asp Trp Gln Arg Leu Ile Asn Asn Asn Trp
290 295 300
Gly Phe Arg Pro Lys Arg Leu Asn Phe Lys Leu Phe Asn Ile Gln Val
305 310 315 320
Lys Glu Val Thr Gln Asn Asp Gly Thr Thr Thr Ile Ala Asn Asn Leu
325 330 335
Thr Ser Thr Val Gln Val Phe Thr Asp Ser Glu Tyr Gln Leu Pro Tyr
340 345 350
Val Leu Gly Ser Ala His Gln Gly Cys Leu Pro Pro Phe Pro Ala Asp
355 360 365
Val Phe Met Val Pro Gln Tyr Gly Tyr Leu Thr Leu Asn Asn Gly Ser
370 375 380
Gln Ala Ala Gly Arg Ser Ser Phe Tyr Cys Leu Glu Tyr Phe Pro Ser
385 390 395 400
Gln Met Leu Arg Thr Gly Asn Asn Phe Thr Phe Ser Tyr Thr Phe Glu
405 410 415
Asp Val Pro Phe His Ser Ser Tyr Ala His Ser Gln Ser Leu Gly Arg
420 425 430
Leu Met Asn Pro Leu Ile Asp Gln Tyr Leu Tyr Tyr Leu Ser Arg Thr
435 440 445
Asn Thr Pro Ser Gly Thr Thr Thr Gln Ser Arg Leu Gln Phe Ser Gln
450 455 460
Ala Gly Ala Ser Asp Ile Arg Asp Gln Ser Arg Asn Trp Leu Pro Gly
465 470 475 480
Pro Cys Tyr Arg Gln Gln Arg Val Ser Lys Thr Ser Ala Asp Asn Asn
485 490 495
Asn Ser Glu Tyr Ser Trp Thr Gly Ala Thr Lys Tyr His Leu Asn Gly
500 505 510
Arg Asp Ser Leu Val Asn Pro Gly Pro Ala Met Ala Ser His Lys Asp
515 520 525
Asp Glu Glu Lys Phe Phe Pro Gln Ser Gly Val Leu Ile Phe Gly Lys
530 535 540
Gln Gly Ser Glu Lys Thr Asn Val Asp Ile Glu Lys Val Met Ile Thr
545 550 555 560
Asp Glu Glu Glu Ile Arg Thr Thr Asn Pro Val Ala Thr Glu Gln Tyr
565 570 575
Gly Ser Val Ser Thr Asn Leu Gln Arg Gly Asn Arg Gln Ala Ala Thr
580 585 590
Ala Asp Val Asn Thr Gln Gly Val Leu Pro Gly Met Val Trp Gln Asp
595 600 605
Arg Asp Val Tyr Leu Gln Gly Pro Ile Trp Ala Lys Ile Pro His Thr
610 615 620
Asp Gly His Phe His Pro Ser Pro Leu Met Gly Gly Phe Gly Leu Lys
625 630 635 640
His Pro Pro Pro Gln Ile Leu Ile Lys Asn Thr Pro Val Pro Ala Asn
645 650 655
Pro Ser Thr Thr Phe Ser Ala Ala Lys Phe Ala Ser Phe Ile Thr Gln
660 665 670
Tyr Ser Thr Gly Gln Val Ser Val Glu Ile Glu Trp Glu Leu Gln Lys
675 680 685
Glu Asn Ser Lys Arg Trp Asn Pro Glu Ile Gln Tyr Thr Ser Asn Tyr
690 695 700
Asn Lys Ser Val Asn Val Asp Phe Thr Val Asp Thr Asn Gly Val Tyr
705 710 715 720
Ser Glu Pro Arg Pro Ile Gly Thr Arg Tyr Leu Thr Arg Asn Leu
725 730 735
<210>127
<211>735
<212>PRT
<213>vp1,克隆hu.45
<400>127
Met Ala Ala Asp Gly Tyr Leu Pro Asp Trp Leu Glu Asp Thr Leu Ser
1 5 10 15
Glu Gly Ile Arg Gln Trp Trp Lys Leu Lys Pro Gly Pro Pro Pro Pro
20 25 30
Lys Pro Ala Glu Arg His Arg Asp Asp Ser Arg Gly Leu Val Leu Pro
35 40 45
Gly Tyr Lys Tyr Leu Gly Pro Phe Asn Gly Leu Asp Lys Gly Glu Pro
50 55 60
Val Asn Glu Ala Asp Ala Ala Ala Leu Glu His Asp Lys Ala Tyr Asp
65 70 75 80
Arg Gln Leu Asp Ser Gly Asp Asn Pro Tyr Leu Lys Tyr Asn His Ala
85 90 95
Asp Ala Glu Phe Gln Glu Arg Leu Lys Glu Asp Thr Ser Phe Gly Gly
100 105 110
Asn Leu Gly Arg Ala Val Phe Gln Ala Lys Lys Arg Val Leu Glu Pro
115 120 125
Leu Gly Leu Val Glu Glu Pro Val Lys Thr Ala Pro Gly Lys Lys Arg
130 135 140
Pro Val Glu His Ser Pro Val Glu Pro Asp Ser Ser Ser Gly Thr Gly
145 150 155 160
Lys Ala Gly Gln Gln Pro Ala Arg Lys Arg Leu Asn Phe Gly Gln Thr
165 170 175
Gly Asp Ala Asp Ser Val Pro Asp Pro Gln Pro Leu Gly Gln Pro Pro
180 185 190
Ala Ala Pro Ser Gly Leu Gly Thr Asn Thr Met Ala Thr Gly Ser Gly
195 200 205
Ala Pro Met Ala Asp Asn Asn Glu Gly Ala Asp Gly Val Gly Asn Ser
210 215 220
Ser Gly Asn Trp His Cys Asp Ser Thr Trp Met Gly Asp Arg Val Ile
225 230 235 240
Thr Thr Ser Thr Arg Thr Trp Ala Leu Pro Thr Tyr Asn Asn His Leu
245 250 255
Tyr Lys Gln Ile Ser Ser Gln Ser Gly Ala Ser Asn Asp Asn His Tyr
260 265 270
Phe Gly Tyr Ser Thr Pro Trp Gly Tyr Phe Asp Phe Asn Arg Phe His
275 280 285
Cys His Phe Ser Pro Arg Asp Trp Gln Arg Leu Ile Asn Asn Asn Trp
290 295 300
Gly Phe Arg Pro Lys Arg Leu Asn Phe Lys Leu Phe Asn Ile Gln Val
305 310 315 320
Lys Glu Val Thr Gln Asn Asp Gly Thr Thr Thr Ile Ala Asn Asn Leu
325 330 335
Thr Ser Thr Val Gln Val Phe Thr Asp Ser Gly Tyr Gln Leu Pro Tyr
340 345 350
Val Leu Gly Ser Ala His Gln Gly Cys Leu Pro Pro Phe Pro Ala Asp
355 360 365
Val Phe Met Val Pro Gln Tyr Gly Tyr Pro Thr Leu Asn Asn Gly Ser
370 375 380
Gln Ala Val Gly Arg Ser Ser Phe Tyr Cys Leu Glu Tyr Phe Pro Ser
385 390 395 400
Gln Met Leu Arg Thr Gly Asn Asn Phe Thr Phe Ser Tyr Thr Phe Glu
405 410 415
Asp Val Pro Phe His Ser Ser Tyr Ala His Ser Gln Ser Leu Asp Arg
420 425 430
Leu Met Asn Pro Leu Ile Asp Gln Tyr Leu Tyr Tyr Leu Ser Thr Thr
435 440 445
Asn Thr Pro Ser Gly Thr Thr Thr Gln Ser Arg Leu Gln Phe Ser Gln
450 455 460
Ala Gly Ala Ser Asp Ile Arg Asp Gln Ser Arg Asn Trp Leu Pro Gly
465 470 475 480
Pro Cys Tyr Arg Gln Gln Arg Val Ser Lys Thr Ser Ala Asp Asn Asn
485 490 495
Asn Ser Glu Tyr Ser Trp Thr Gly Ala Thr Lys Tyr His Leu Asn Gly
500 505 510
Arg Asp Ser Leu Val Asn Pro Gly Pro Ala Val Ala Ser His Lys Asp
515 520 525
Asp Glu Glu Lys Phe Phe Pro Gln Ser Gly Val Leu Ile Phe Gly Lys
530 535 540
Gln Gly Ser Glu Lys Thr Asn Val Asp Ile Glu Lys Val Met Ile Thr
545 550 555 560
Asp Glu Glu Glu Ile Arg Thr Thr Asn Pro Val Ala Thr Glu Gln Tyr
565 570 575
Gly Ser Val Ser Thr Asn Leu Gln Arg Gly Asn Arg Gln Ala Ala Thr
580 585 590
Ala Asp Val Asn Thr Gln Gly Val Leu Pro Gly Met Val Trp Gln Asp
595 600 605
Arg Asp Val Tyr Leu Gln Gly Pro Ile Trp Ala Lys Ile Pro His Thr
610 615 620
Asp Gly His Phe His Pro Ser Pro Leu Met Gly Gly Phe Gly Leu Lys
625 630 635 640
His Pro Pro Pro Gln Ile Leu Ile Lys Asn Thr Pro Val Pro Ala Asn
645 650 655
Pro Ser Thr Thr Phe Ser Ala Ala Lys Phe Ala Ser Phe Ile Thr Gln
660 665 670
Tyr Ser Thr Gly Gln Val Ser Val Glu Ile Glu Trp Glu Leu Gln Lys
675 680 685
Glu Asn Ser Lys Arg Trp Asn Pro Glu Ile Gln Tyr Thr Ser Asn Tyr
690 695 700
Asn Lys Ser Val Asn Val Asp Phe Thr Val Asp Thr Asn Gly Val Tyr
705 710 715 720
Ser Glu Pro Arg Pro Ile Gly Thr Arg Tyr Leu Thr Arg Asn Leu
725 730 735
<210>128
<211>735
<212>PRT
<213>vp1,克隆hu.47
<400>128
Met Ala Ala Asp Gly Tyr Leu Pro Asp Trp Leu Glu Asp Thr Leu Ser
1 5 10 15
Glu Gly Ile Arg Gln Trp Trp Lys Leu Lys Pro Gly Pro Pro Pro Pro
20 25 30
Lys Pro Ala Glu Arg His Arg Asp Asp Ser Arg Gly Leu Val Leu Pro
35 40 45
Gly Tyr Lys Tyr Leu Gly Pro Phe Asn Gly Leu Asp Lys Gly Glu Pro
50 55 60
Val Asn Glu Ala Asp Ala Ala Ala Leu Glu His Asp Lys Ala Tyr Asp
65 70 75 80
Arg Gln Leu Asp Ser Gly Asp Asn Pro Tyr Leu Lys Tyr Asn His Ala
85 90 95
Asp Ala Glu Phe Gln Glu Arg Leu Lys Glu Asp Thr Ser Phe Gly Gly
100 105 110
Asn Leu Gly Arg Ala Val Phe Gln Ala Lys Lys Arg Val Leu Glu Pro
115 120 125
Leu Gly Leu Val Gly Glu Pro Val Lys Thr Ala Pro Gly Lys Lys Arg
130 135 140
Pro Val Glu His Ser Pro Val Glu Pro Asp Ser Ser Ser Gly Thr Gly
145 150 155 160
Lys Ala Gly Gln Gln Pro Ala Arg Lys Arg Leu Asn Phe Gly Gln Thr
165 170 175
Gly Asp Ala Asp Ser Val Pro Asp Pro Gln Pro Leu Gly Gln Pro Pro
180 185 190
Ala Ala Pro Ser Gly Leu Gly Thr Asn Thr Met Ala Thr Gly Ser Gly
195 200 205
Ala Pro Met Ala Asp Asn Asn Glu Gly Ala Asp Gly Val Gly Asn Ser
210 215 220
Ser Gly Asn Trp His Cys Asp Ser Thr Trp Met Gly Asp Arg Val Ile
225 230 235 240
Thr Thr Ser Thr Arg Thr Trp Ala Leu Pro Thr Tyr Asn Asn His Leu
245 250 255
Tyr Lys Gln Ile Ser Ser Gln Ser Gly Ala Ser Asn Asp Ser His Tyr
260 265 270
Phe Gly Tyr Ser Thr Pro Trp Gly Tyr Phe Asp Phe Asn Arg Phe His
275 280 285
Cys His Phe Ser Pro Arg Asp Trp Gln Arg Leu Ile Asn Asn Asn Trp
290 295 300
Gly Phe Arg Pro Lys Arg Leu Asn Phe Lys Leu Phe Asn Ile Gln Val
305 310 315 320
Lys Glu Val Thr Gln Asn Asp Gly Thr Thr Thr Ile Ala Asn Asn Leu
325 330 335
Thr Ser Thr Val Gln Val Phe Thr Asp Ser Glu Tyr Gln Leu Pro Tyr
340 345 350
Val Leu Gly Ser Ala His Gln Gly Cys Leu Pro Pro Phe Pro Ala Asp
355 360 365
Val Phe Met Val Pro Gln Tyr Gly Tyr Leu Thr Leu Asn Asn Gly Ser
370 375 380
Gln Ala Val Gly Arg Ser Ser Phe Tyr Cys Leu Glu Tyr Phe Pro Ser
385 390 395 400
Gln Met Leu Arg Thr Gly Asn Asn Phe Thr Phe Ser Tyr Thr Phe Glu
405 410 415
Asp Val Pro Phe His Ser Ser Tyr Ala His Ser Gln Ser Leu Asp Arg
420 425 430
Leu Met Asn Pro Leu Ile Asp Gln Tyr Leu Tyr Tyr Leu Ser Thr Thr
435 440 445
Asn Thr Pro Ser Gly Thr Thr Thr Gln Ser Arg Leu Gln Phe Ser Gln
450 455 460
Ala Gly Ala Ser Asp Ile Arg Asp Gln Ser Arg Asn Trp Leu Pro Gly
465 470 475 480
Pro Cys Tyr Arg Gln Gln Arg Val Ser Lys Thr Ser Ala Asp Asn Asn
485 490 495
Asn Ser Glu Tyr Ser Trp Thr Gly Ala Thr Lys Tyr His Leu Asn Gly
500 505 510
Arg Asp Ser Leu Val Asn Pro Gly Pro Ala Met Ala Ser His Lys Asp
515 520 525
Asn Glu Glu Lys Phe Phe Pro Gln Ser Gly Val Leu Ile Phe Gly Lys
530 535 540
Gln Gly Ser Glu Lys Thr Asn Val Asp Ile Glu Lys Val Met Ile Thr
545 550 555 560
Asp Glu Glu Glu Ile Arg Thr Thr Asn Pro Val Ala Thr Glu Gln Tyr
565 570 575
Gly Ser Val Ser Thr Asn Leu Gln Arg Gly Asn Arg Gln Ala Ala Thr
580 585 590
Ala Asp Val Asn Thr Gln Gly Val Leu Pro Gly Met Val Trp Gln Asp
595 600 605
Arg Asp Val Tyr Leu Gln Gly Pro Ile Trp Ala Lys Ile Pro His Thr
610 615 620
Asp Gly His Phe His Pro Ser Pro Leu Met Gly Gly Phe Gly Leu Lys
625 630 635 640
His Pro Pro Pro Gln Ile Leu Ile Lys Asn Thr Pro Val Pro Ala Asn
645 650 655
Pro Ser Thr Thr Phe Ser Ala Ala Lys Phe Ala Ser Phe Ile Thr Gln
660 665 670
Tyr Ser Thr Gly Gln Val Ser Val Glu Ile Glu Trp Glu Leu Gln Lys
675 680 685
Glu Asn Ser Lys Arg Trp Asn Pro Glu Ile Gln Tyr Thr Ser Asn Tyr
690 695 700
Asn Lys Ser Val Asn Val Asp Phe Thr Val Asp Thr Asn Gly Val Tyr
705 710 715 720
Ser Glu Pro Arg Pro Ile Gly Thr Arg Tyr Leu Thr Arg Asn Leu
725 730 735
<210>129
<211>735
<212>PRT
<213>vp1,克隆hu.13
<400>129
Met Ala Ala Asp Gly Tyr Leu Pro Asp Trp Leu Glu Asp Thr Leu Ser
1 5 10 15
Glu Gly Ile Arg Gln Trp Trp Lys Leu Lys Pro Gly Pro Pro Pro Pro
20 25 30
Lys Pro Ala Glu Arg His Lys Asp Asp Ser Arg Gly Leu Val Leu Pro
35 40 45
Gly Tyr Lys Tyr Leu Gly Pro Phe Asn Gly Leu Asp Lys Gly Glu Pro
50 55 60
Val Asn Glu Ala Asp Ala Ala Ala Leu Glu His Asp Lys Ala Tyr Asp
65 70 75 80
Arg Gln Leu Asp Ser Gly Asp Asn Pro Tyr Leu Lys Tyr Asn His Ala
85 90 95
Asp Ala Glu Phe Gln Glu Arg Leu Lys Glu Asp Thr Ser Phe Gly Gly
100 105 110
Asn Leu Gly Arg Ala Val Phe Gln Ala Lys Lys Arg Val Leu Glu Pro
115 120 125
Leu Gly Leu Val Glu Glu Pro Val Lys Thr Ala Pro Gly Lys Lys Arg
130 135 140
Pro Val Glu His Ser Pro Ala Glu Pro Asp Ser Ser Ser Gly Thr Gly
145 150 155 160
Lys Ala Gly Gln Gln Pro Ala Arg Lys Arg Leu Asn Phe Gly Gln Thr
165 170 175
Gly Asp Ala Asp Ser Val Pro Asp Pro Gln Pro Leu Gly Gln Pro Pro
180 185 190
Ala Ala Pro Ser Gly Leu Gly Thr Asn Thr Met Ala Ser Gly Ser Gly
195 200 205
Ala Pro Met Ala Asp Asn Asn Glu Gly Ala Asp Gly Val Gly Asn Ser
210 215 220
Ser Gly Asn Trp His Cys Asp Ser Thr Trp Met Gly Asp Arg Val Ile
225 230 235 240
Thr Thr Ser Thr Arg Thr Trp Ala Leu Pro Thr Tyr Asn Asn His Leu
245 250 255
Tyr Lys Gln Ile Ser Ser Gln Ser Gly Ala Ser Asn Asp Asn His Tyr
260 265 270
Phe Gly Tyr Ser Thr Pro Trp Gly Tyr Phe Asp Phe Asn Arg Phe His
275 280 285
Cys His Phe Ser Pro Arg Asp Trp Gln Arg Leu Ile Asn Asn Asn Trp
290 295 300
Gly Phe Arg Pro Lys Arg Leu Asn Phe Lys Leu Phe Asn Ile Gln Val
305 310 315 320
Lys Glu Val Thr Gln Asn Asp Gly Thr Thr Thr Ile Ala Asn Asn Leu
325 330 335
Thr Ser Thr Val Gln Val Phe Thr Asp Ser Glu Tyr Gln Leu Pro Tyr
340 345 350
Val Leu Gly Ser Ala His Gln Gly Cys Leu Pro Pro Phe Pro Ala Asp
355 360 365
Val Phe Met Val Pro Gln Tyr Gly Tyr Leu Thr Leu Asn Asn Gly Ser
370 375 380
Gln Ala Val Gly Arg Ser Ser Phe Tyr Cys Leu Glu Tyr Phe Pro Ser
385 390 395 400
Gln Met Leu Arg Thr Gly Asn Asn Phe Thr Phe Ser Tyr Thr Phe Glu
405 410 415
Asp Val Pro Phe His Ser Ser Tyr Ala His Ser Gln Ser Leu Asp Arg
420 425 430
Leu Met Asn Pro Leu Ile Asp Gln Tyr Leu Tyr Tyr Leu Ser Arg Thr
435 440 445
Asn Thr Pro Ser Gly Thr Thr Thr Gln Ser Arg Leu Gln Phe Ser Gln
450 455 460
Ala Gly Ala Ser Asp Ile Arg Asp Gln Ser Arg Asn Trp Leu Pro Gly
465 470 475 480
Pro Cys Tyr Arg Gln Gln Arg Val Ser Lys Thr Ser Ala Asp Asn Asn
485 490 495
Asn Ser Glu Tyr Ser Trp Thr Gly Ala Thr Lys Tyr His Leu Asn Gly
500 505 510
Arg Asp Ser Leu Val Asn Pro Gly Pro Ala Met Ala Ser His Lys Asp
515 520 525
Asp Glu Glu Lys Phe Phe Pro Gln Ser Gly Val Leu Ile Phe Gly Lys
530 535 540
Gln Gly Ser Glu Lys Thr Asn Val Asp Ile Glu Lys Val Met Ile Thr
545 550 555 560
Asp Glu Glu Glu Ile Arg Thr Thr Asn Pro Val Ala Thr Glu Gln Tyr
565 570 575
Gly Ser Val Ser Thr Asn Leu Gln Gly Gly Asn Thr Gln Ala Ala Thr
580 585 590
Ala Asp Val Asn Thr Gln Gly Val Leu Pro Gly Met Val Trp Gln Asp
595 600 605
Arg Asp Val Tyr Leu Gln Gly Pro Ile Trp Ala Lys Ile Pro His Thr
610 615 620
Asp Gly His Phe His Pro Ser Pro Leu Met Gly Gly Phe Gly Leu Lys
625 630 635 640
His Pro Pro Pro Gln Ile Leu Ile Lys Asn Thr Pro Val Pro Ala Asn
645 650 655
Pro Ser Thr Thr Phe Ser Ala Ala Lys Phe Ala Ser Phe Ile Thr Gln
660 665 670
Tyr Ser Thr Gly Gln Val Ser Val Glu Ile Glu Trp Glu Leu Gln Lys
675 680 685
Glu Asn Ser Lys Arg Trp Asn Pro Glu Ile Gln Tyr Thr Ser Asn Tyr
690 695 700
Asn Lys Ser Val Asn Val Asp Phe Thr Val Asp Thr Asn Gly Val Tyr
705 710 715 720
Ser Glu Pro Arg Pro Ile Gly Thr Arg Tyr Leu Thr Arg Asn Leu
725 730 735
<210>130
<211>735
<212>PRT
<213>vp1,克隆hu.28
<400>130
Met Ala Ala Asp Gly Tyr Leu Pro Asp Trp Leu Glu Asp Thr Leu Ser
1 5 10 15
Glu Gly Ile Arg Gln Trp Trp Lys Leu Lys Pro Gly Pro Pro Pro Pro
20 25 30
Lys Pro Ala Glu Arg His Lys Asp Asp Ser Arg Gly Leu Val Leu Pro
35 40 45
Gly Tyr Lys Tyr Leu Gly Pro Phe Asn Gly Leu Asp Lys Gly Glu Pro
50 55 60
Val Asn Glu Ala Asp Ala Ala Ala Leu Glu His Asp Lys Ala Tyr Asp
65 70 75 80
Arg Gln Leu Asp Ser Gly Asp Asn Pro Tyr Leu Lys Tyr Asn His Ala
85 90 95
Asp Ala Glu Phe Gln Glu Arg Leu Lys Glu Asp Thr Ser Phe Gly Gly
100 105 110
Asn Leu Gly Arg Ala Val Phe Gln Ala Lys Lys Arg Val Leu Glu Pro
115 120 125
Leu Ser Leu Val Glu Glu Pro Val Lys Thr Ala Pro Gly Lys Lys Arg
130 135 140
Pro Val Glu His Ser Pro Ala Glu Pro Asp Ser Ser Ser Gly Thr Gly
145 150 155 160
Lys Ser Gly Asn Gln Pro Ala Arg Lys Arg Leu Asn Phe Gly Gln Thr
165 170 175
Gly Asp Ser Asp Ser Val Pro Asp Pro Gln Pro Leu Gly Gln Pro Pro
180 185 190
Ala Ala Pro Ser Gly Leu Gly Thr Asn Thr Met Ala Thr Gly Ser Gly
195 200 205
Ala Pro Met Ala Asp Asn Asn Glu Gly Ala Asp Gly Val Gly Asn Ser
210 215 220
Ser Gly Asn Trp His Cys Asp Ser Thr Trp Met Gly Asp Arg Val Ile
225 230 235 240
Thr Thr Ser Thr Arg Thr Trp Ala Leu Pro Thr Tyr Asn Asn His Leu
245 250 255
Tyr Lys Gln Ile Ser Ser Gln Ser Gly Ala Ser Asn Asp Asn His Tyr
260 265 270
Phe Gly Tyr Ser Thr Pro Trp Gly Tyr Phe Asp Phe Asn Arg Phe His
275 280 285
Cys His Phe Ser Pro Arg Asp Trp Gln Arg Leu Ile Asn Asn Asn Trp
290 295 300
Gly Phe Arg Pro Lys Arg Leu Asn Phe Lys Leu Phe Asn Ile Gln Val
305 310 315 320
Lys Glu Val Thr Gln Asn Asp Gly Thr Thr Thr Ile Ala Asn Asn Leu
325 330 335
Thr Ser Thr Val Gln Val Phe Thr Asp Ser Glu Tyr Gln Leu Pro Tyr
340 345 350
Val Leu Gly Ser Ala His Gln Gly Cys Leu Pro Pro Phe Pro Ala Asp
355 360 365
Val Phe Met Val Pro Gln Tyr Gly Tyr Leu Thr Leu Asn Asn Gly Ser
370 375 380
Gln Ala Val Gly Arg Ser Ser Phe Tyr Cys Leu Glu Tyr Phe Pro Ser
385 390 395 400
Gln Met Leu Arg Thr Gly Asn Asn Phe Thr Phe Ser Tyr Thr Phe Glu
405 410 415
Asp Val Pro Phe His Ser Ser Tyr Ala His Ser Gln Ser Leu Asp Arg
420 425 430
Leu Met Asn Pro Leu Ile Asp Gln Tyr Leu Tyr Tyr Leu Ser Arg Thr
435 440 445
Asn Thr Pro Ser Gly Thr Thr Thr Gln Ser Arg Leu Gln Phe Ser Gln
450 455 460
Ala Gly Ala Ser Asp Ile Gln Asp Gln Ser Arg Asn Trp Leu Pro Gly
465 470 475 480
Pro Cys Tyr Arg Gln Gln Arg Val Ser Lys Thr Ser Ala Asp Asn Asn
485 490 495
Asn Ser Glu Tyr Ser Trp Thr Gly Ala Thr Lys Tyr His Leu Asn Gly
500 505 510
Arg Asp Ser Leu Val Asn Pro Gly Pro Ala Met Ala Ser His Lys Asp
515 520 525
Asp Glu Glu Lys Phe Phe Pro Gln Ser Gly Val Leu Ile Phe Gly Lys
530 535 540
Gln Gly Ser Glu Lys Thr Asn Val Asp Ile Glu Lys Val Met Ile Thr
545 550 555 560
Asp Glu Glu Glu Ile Arg Thr Thr Asn Pro Val Ala Thr Glu Gln Tyr
565 570 575
Gly Ser Val Ser Thr Asn Leu Gln Ser Gly Asn Thr Gln Ala Ala Thr
580 585 590
Ala Asp Val Asn Thr Gln Gly Val Leu Pro Gly Met Val Gly Gln Asp
595 600 605
Arg Asp Val Tyr Leu Gln Gly Pro Thr Trp Ala Lys Ile Pro His Thr
610 615 620
Asp Gly His Phe His Pro Ser Pro Leu Met Gly Gly Phe Gly Leu Lys
625 630 635 640
His Pro Pro Pro Gln Ile Leu Ile Lys Asn Thr Pro Val Pro Ala Asn
645 650 655
Pro Ser Thr Thr Phe Ser Ala Ala Lys Phe Ala Ser Phe Ile Thr Gln
660 665 670
Tyr Ser Thr Gly Gln Val Ser Val Glu Ile Glu Trp Glu Leu Gln Lys
675 680 685
Glu Asn Ser Lys Arg Trp Asn Pro Glu Ile Gln Tyr Thr Ser Asn Tyr
690 695 700
Asn Lys Ser Val Asn Val Asp Phe Thr Val Asp Thr Asn Gly Val Tyr
705 710 715 720
Ser Glu Pro Arg Pro Ile Gly Thr Arg Tyr Leu Thr Arg Asn Leu
725 730 735
<210>131
<211>735
<212>PRT
<213>vp1,克隆hu.30
<400>131
Met Ala Ala Asp Gly Tyr Leu Pro Asp Trp Leu Glu Asp Thr Leu Ser
1 5 10 15
Glu Gly Ile Arg Gln Trp Trp Lys Leu Lys Pro Gly Pro Pro Pro Pro
20 25 30
Lys Pro Ala Glu Arg His Lys Asp Asp Ser Arg Gly Leu Val Leu Pro
35 40 45
Gly Tyr Lys Tyr Leu Gly Pro Phe Asn Gly Leu Asp Lys Gly Glu Pro
50 55 60
Val Asn Glu Ala Asp Ala Ala Ala Leu Glu His Asp Lys Ala Tyr Asp
65 70 75 80
Arg Gln Leu Asp Ser Gly Asp Asn Pro Tyr Leu Lys Tyr Asn His Ala
85 90 95
Asp Ala Glu Phe Gln Glu Arg Leu Lys Glu Asp Thr Ser Phe Gly Gly
100 105 110
Asn Leu Gly Arg Ala Val Phe Gln Ala Lys Lys Arg Val Leu Glu Pro
115 120 125
Leu Gly Leu Val Glu Glu Pro Val Lys Thr Ala Pro Gly Lys Lys Arg
130 135 140
Pro Val Glu His Ser Pro Ala Glu Pro Asp Ser Ser Ser Gly Thr Gly
145 150 155 160
Lys Ser Gly Asn Gln Pro Ala Arg Lys Arg Leu Asn Phe Gly Gln Thr
165 170 175
Gly Asp Ser Asp Ser Val Pro Asp Pro Gln Pro Leu Gly Gln Pro Pro
180 185 190
Ala Ala Pro Ser Gly Leu Gly Thr Asn Thr Met Ala Thr Gly Ser Gly
195 200 205
Ala Pro Met Ala Asp Asn Asn Glu Gly Ala Asp Gly Val Gly Asn Ser
210 215 220
Ser Gly Asn Trp His Cys Asp Ser Thr Trp Met Gly Asp Arg Val Ile
225 230 235 240
Thr Thr Ser Thr Arg Thr Trp Ala Leu Pro Thr Tyr Asn Asn His Leu
245 250 255
Tyr Lys Gln Ile Ser Ser Gln Ser Gly Ala Ser Asn Asp Asn His Tyr
260 265 270
Phe Gly Tyr Ser Thr Pro Trp Gly Tyr Phe Asp Phe Asn Arg Phe His
275 280 285
Cys His Phe Ser Pro Arg Asp Trp Gln Arg Leu Ile Asn Asn Asn Trp
290 295 300
Gly Phe Arg Pro Lys Arg Leu Asn Phe Lys Leu Phe Asn Ile Gln Val
305 310 315 320
Lys Glu Val Thr Gln Asn Asp Gly Thr Thr Thr Ile Ala Asn Asn Leu
325 330 335
Thr Ser Thr Val Gln Val Phe Thr Asp Ser Glu Tyr Gln Leu Pro Tyr
340 345 350
Val Leu Gly Ser Ala His Gln Gly Cys Leu Pro Pro Phe Pro Ala Asp
355 360 365
Val Phe Met Val Pro Gln Tyr Gly Tyr Leu Thr Leu Asn Asn Gly Ser
370 375 380
Gln Ala Val Gly Arg Ser Ser Phe Tyr Cys Leu Glu Tyr Phe Pro Ser
385 390 395 400
Gln Met Leu Arg Thr Gly Asn Ser Phe Thr Phe Ser Tyr Thr Phe Glu
405 410 415
Asp Val Pro Phe His Ser Ser Tyr Ala His Ser Gln Ser Leu Asp Arg
420 425 430
Leu Met Asn Pro Leu Ile Asp Gln Tyr Leu Tyr Tyr Leu Ser Arg Thr
435 440 445
Asn Thr Pro Ser Gly Thr Thr Thr Gln Ser Arg Leu Gln Phe Ser Gln
450 455 460
Ala Gly Ala Ser Asp Ile Arg Asp Gln Ser Arg Asn Trp Leu Pro Gly
465 470 475 480
Pro Cys Tyr Arg Gln Gln Arg Val Ser Lys Thr Ser Ala Asp Asn Asn
485 490 495
Asn Ser Glu Tyr Ser Trp Thr Gly Ala Thr Lys Tyr His Leu Asn Gly
500 505 510
Arg Asp Ser Leu Val Asn Pro Gly Pro Ala Met Ala Ser His Lys Asp
515 520 525
Asp Glu Glu Lys Phe Phe Pro Gln Ser Gly Val Leu Ile Phe Gly Lys
530 535 540
Gln Gly Ser Glu Lys Thr Asn Val Asp Ile Glu Lys Val Met Ile Thr
545 550 555 560
Asp Glu Glu Glu Ile Arg Thr Thr Asn Pro Val Ala Thr Glu Gln Tyr
565 570 575
Gly Ser Val Ser Thr Asn Leu Gln Ser Gly Asn Thr Gln Ala Ala Thr
580 585 590
Ala Asp Val Asn Thr Gln Gly Val Leu Pro Gly Met Val Trp Gln Asp
595 600 605
Arg Asp Val Tyr Leu Gln Gly Pro Ile Trp Ala Lys Ile Pro His Thr
610 615 620
Asp Gly His Phe His Pro Ser Pro Leu Met Gly Gly Phe Gly Leu Lys
625 630 635 640
His Pro Pro Pro Gln Ile Leu Ile Lys Asn Thr Pro Val Pro Ala Asn
645 650 655
Pro Ser Thr Thr Phe Ser Ala Ala Lys Phe Ala Ser Phe Ile Thr Gln
660 665 670
Tyr Ser Thr Gly Gln Val Ser Val Glu Ile Glu Trp Glu Leu Gln Lys
675 680 685
Glu Asn Ser Lys Arg Trp Asn Pro Glu Ile Gln Tyr Thr Ser Asn Tyr
690 695 700
Asn Lys Ser Val Asn Val Asp Phe Thr Val Asp Thr Asn Gly Val Tyr
705 710 715 720
Ser Glu Pro Arg Pro Ile Gly Thr Arg Tyr Leu Thr Arg Asn Leu
725 730 735
<210>132
<211>735
<212>PRT
<213>vp1,克隆hu.29
<400>132
Met Ala Ala Asp Gly Tyr Leu Pro Asp Trp Leu Glu Asp Thr Leu Ser
1 5 10 15
Glu Gly Ile Arg Gln Trp Trp Lys Leu Lys Pro Gly Pro Pro Pro Pro
20 25 30
Lys Pro Ala Glu Arg His Lys Asp Asp Ser Arg Gly Leu Val Leu Pro
35 40 45
Gly Tyr Lys Tyr Leu Gly Pro Phe Asn Gly Leu Asp Lys Gly Glu Pro
50 55 60
Val Asn Glu Ala Asp Ala Ala Ala Leu Glu His Asp Lys Ala Tyr Asp
65 70 75 80
Arg Gln Leu Asp Ser Gly Asp Asn Pro Tyr Leu Lys Tyr Asn His Ala
85 90 95
Asp Ala Glu Phe Gln Glu Arg Leu Lys Glu Asp Thr Ser Phe Gly Gly
100 105 110
Asn Leu Gly Arg Ala Val Phe Gln Ala Lys Lys Arg Val Leu Glu Pro
115 120 125
Leu Gly Leu Val Glu Glu Pro Val Lys Thr Ala Pro Gly Lys Lys Arg
130 135 140
Pro Val Glu His Ser Pro Ala Glu Pro Asp Ser Ser Ser Gly Thr Gly
145 150 155 160
Lys Ser Gly Asn Gln Pro Ala Arg Lys Arg Leu Asn Phe Gly Gln Thr
165 170 175
Gly Asp Ser Asp Ser Val Pro Asp Pro Gln Pro Leu Gly Gln Pro Pro
180 185 190
Ala Ala Pro Ser Gly Leu Gly Thr Asn Thr Met Ala Thr Gly Ser Gly
195 200 205
Ala Pro Met Ala Asp Asn Asn Glu Gly Ala Asp Gly Val Gly Asn Ser
210 215 220
Ser Gly Asn Trp His Cys Asp Ser Thr Trp Met Gly Asp Arg Val Ile
225 230 235 240
Thr Thr Ser Thr Arg Thr Trp Ala Leu Pro Thr Tyr Asn Asn His Leu
245 250 255
Tyr Lys Gln Ile Ser Ser Gln Ser Gly Ala Ser Asn Asp Asn His Tyr
260 265 270
Phe Gly Tyr Ser Thr Pro Trp Gly Tyr Phe Asp Phe Asn Arg Phe His
275 280 285
Cys His Phe Ser Pro Arg Asp Trp Gln Arg Leu Ile Asn Asn Asn Trp
290 295 300
Gly Phe Arg Pro Lys Arg Leu Asn Phe Lys Leu Phe Asn Ile Gln Val
305 310 315 320
Lys Glu Val Thr Gln Asn Asp Gly Thr Thr Thr Ile Ala Asn Asn Leu
325 330 335
Thr Ser Thr Val Gln Val Phe Thr Asp Ser Glu Tyr Gln Leu Pro Tyr
340 345 350
Val Leu Gly Ser Ala His Gln Gly Cys Leu Pro Pro Phe Pro Ala Asp
355 360 365
Val Phe Met Val Pro Gln Tyr Gly Tyr Leu Thr Leu Asn Asn Gly Ser
370 375 380
Gln Ala Val Gly Arg Ser Ser Phe Tyr Cys Leu Gly Tyr Phe Pro Ser
385 390 395 400
Gln Met Leu Arg Thr Gly Asn Asn Phe Thr Phe Ser Tyr Thr Phe Glu
405 410 415
Asp Val Pro Phe His Ser Ser Tyr Ala His Ser Gln Ser Leu Asp Arg
420 425 430
Leu Met Asn Pro Leu Ile Asp Gln Tyr Leu Tyr Tyr Leu Ser Arg Thr
435 440 445
Asn Thr Pro Ser Gly Thr Thr Thr Gln Ser Arg Leu Gln Phe Ser Gln
450 455 460
Ala Gly Ala Ser Asp Ile Arg Asp Gln Ser Arg Asn Trp Leu Pro Gly
465 470 475 480
Pro Cys Tyr Arg Gln Gln Arg Val Ser Lys Thr Ser Ala Asp Asn Asn
485 490 495
Asn Ser Glu Tyr Ser Trp Thr Gly Ala Thr Lys Tyr His Leu Asn Gly
500 505 510
Arg Asp Ser Leu Val Asn Pro Gly Pro Ala Met Ala Ser His Lys Asp
515 520 525
Asp Glu Glu Lys Phe Phe Pro Gln Ser Gly Val Leu Ile Phe Gly Lys
530 535 540
Gln Gly Pro Glu Lys Thr Asn Val Asp Ile Glu Lys Val Met Ile Thr
545 550 555 560
Asp Glu Glu Glu Ile Arg Thr Thr Asn Pro Val Ala Thr Glu Gln Tyr
565 570 575
Gly Ser Val Ser Thr Asn Leu Gln Ser Gly Asn Thr Gln Ala Ala Thr
580 585 590
Ala Asp Val Asn Thr Gln Gly Val Leu Pro Gly Met Val Trp Gln Asp
595 600 605
Arg Asp Val Tyr Leu Gln Gly Pro Ile Trp Ala Lys Ile Pro His Thr
610 615 620
Asp Gly His Phe His Pro Ser Pro Leu Met Gly Gly Phe Gly Leu Lys
625 630 635 640
His Pro Pro Pro Gln Ile Leu Ile Lys Asn Thr Pro Val Pro Ala Asn
645 650 655
Pro Ser Thr Thr Phe Ser Ala Ala Lys Phe Ala Ser Phe Ile Thr Gln
660 665 670
Tyr Ser Thr Gly Gln Val Ser Val Glu Ile Glu Trp Glu Leu Gln Lys
675 680 685
Glu Asn Ser Lys Arg Trp Asn Pro Glu Ile Gln Tyr Thr Ser Asn Tyr
690 695 700
Asn Lys Ser Val Asn Val Asp Phe Thr Val Asp Thr Asn Gly Val Tyr
705 710 715 720
Ser Glu Pro Arg Pro Ile Gly Thr Arg Tyr Leu Thr Arg Asn Leu
725 730 735
<210>133
<211>735
<212>PRT
<213>vp1,克隆hu.19
<400>133
Met Ala Ala Asp Gly Tyr Leu Pro Asp Trp Leu Glu Asp Thr Leu Ser
1 5 10 15
Glu Gly Ile Arg Gln Trp Trp Lys Leu Lys Pro Gly Pro Pro Pro Pro
20 25 30
Lys Pro Ala Glu Arg His Lys Asp Asp Ser Arg Gly Leu Val Leu Pro
35 40 45
Gly Tyr Lys Tyr Leu Gly Pro Phe Asn Gly Leu Asp Lys Gly Glu Pro
50 55 60
Val Asn Glu Ala Asp Ala Ala Ala Leu Glu His Asp Lys Ala Tyr Asp
65 70 75 80
Arg Gln Leu Asp Ser Gly Asp Asn Pro Tyr Leu Lys Tyr Asn His Ala
85 90 95
Asp Ala Glu Phe Gln Glu Arg Leu Lys Glu Asp Thr Ser Phe Gly Gly
100 105 110
Asn Leu Gly Arg Ala Val Phe Gln Ala Lys Lys Arg Ile Leu Glu Pro
115 120 125
Leu Gly Leu Val Glu Glu Pro Val Lys Thr Ala Pro Gly Glu Lys Arg
130 135 140
Pro Val Glu His Ser Pro Ala Glu Pro Asp Ser Ser Ser Gly Thr Gly
145 150 155 160
Lys Ala Gly Gln Gln Pro Ala Arg Lys Arg Leu Asn Phe Gly Gln Thr
165 170 175
Gly Asp Ala Asp Ser Val Pro Asp Pro Gln Pro Leu Gly Gln Pro Pro
180 185 190
Ala Ala Pro Ser Gly Leu Gly Thr Asn Thr Met Ala Ser Gly Ser Gly
195 200 205
Ala Pro Met Ala Asp Asn Asn Glu Gly Ala Asp Gly Val Gly Asn Ser
210 215 220
Ser Gly Asn Trp Tyr Cys Asp Ser Thr Trp Met Gly Asp Arg Val Ile
225 230 235 240
Thr Thr Ser Thr Arg Thr Trp Ala Leu Pro Thr Tyr Asn Asn His Leu
245 250 255
Tyr Lys Gln Ile Ser Ser Gln Ser Gly Ala Ser Asn Asp Asn His Tyr
260 265 270
Phe Gly Tyr Ser Thr Pro Trp Gly Tyr Phe Asp Phe Asn Arg Phe His
275 280 285
Cys His Phe Ser Pro Arg Asp Trp Gln Arg Leu Ile Asn Asn Asn Trp
290 295 300
Gly Phe Arg Pro Lys Arg Leu Ser Phe Lys Leu Phe Asn Ile Gln Val
305 310 315 320
Lys Glu Val Thr Gln Asn Asp Gly Thr Thr Thr Ile Ala Asn Asn Leu
325 330 335
Thr Ser Thr Val Gln Val Phe Thr Asp Ser Glu Tyr Gln Leu Pro Tyr
340 345 350
Val Leu Gly Ser Ala His Gln Gly Cys Leu Pro Pro Phe Pro Ala Asp
355 360 365
Val Phe Met Val Pro Gln Tyr Gly Tyr Leu Thr Leu Asn Asn Gly Ser
370 375 380
Gln Ala Val Gly Arg Ser Ser Phe Tyr Cys Leu Glu Tyr Phe Pro Ser
385 390 395 400
Gln Met Leu Arg Thr Gly Asn Asn Phe Thr Phe Ser Tyr Thr Phe Glu
405 410 415
Asp Val Pro Phe His Ser Ser Tyr Ala His Ser Gln Ser Leu Asp Arg
420 425 430
Leu Met Asn Pro Leu Ile Asp Gln Tyr Leu Tyr Tyr Leu Ser Arg Thr
435 440 445
Asn Thr Pro Ser Gly Thr Thr Thr Met Ser Arg Leu Gln Phe Ser Gln
450 455 460
Ala Gly Ala Ser Asp Ile Arg Asp Gln Ser Arg Asn Trp Leu Pro Gly
465 470 475 480
Pro Cys Tyr Arg Gln Gln Arg Val Ser Lys Thr Ala Ala Asp Asn Asn
485 490 495
Asn Ser Asp Tyr Ser Trp Thr Gly Ala Thr Lys Tyr His Leu Asn Gly
500 505 510
Arg Asp Ser Leu Val Asn Pro Gly Pro Ala Met Ala Ser His Lys Asp
515 520 525
Asp Glu Glu Lys Tyr Phe Pro Gln Ser Gly Val Leu Ile Phe Gly Lys
530 535 540
Gln Asp Ser Gly Lys Thr Asn Val Asp Ile Glu Lys Val Met Ile Thr
545 550 555 560
Asp Glu Glu Glu Ile Arg Thr Thr Asn Pro Val Ala Thr Glu Gln Tyr
565 570 575
Gly Ser Val Ser Thr Asn Leu Gln Ser Gly Asn Thr Gln Ala Ala Thr
580 585 590
Ser Asp Val Asn Thr Gln Gly Val Leu Pro Gly Met Val Trp Gln Asp
595 600 605
Arg Asp Val Tyr Leu Gln Gly Pro Ile Trp Ala Lys Ile Pro His Thr
610 615 620
Asp Gly His Phe His Pro Ser Pro Leu Val Gly Gly Phe Gly Leu Lys
625 630 635 640
His Pro Pro Pro Gln Ile Leu Ile Lys Asn Thr Pro Val Pro Ala Asn
645 650 655
Pro Ser Thr Thr Phe Ser Ala Ala Lys Phe Ala Ser Phe Ile Thr Gln
660 665 670
Tyr Ser Thr Gly Gln Val Ser Val Glu Ile Glu Trp Glu Leu Gln Lys
675 680 685
Glu Asn Ser Lys Arg Trp Asn Pro Glu Ile Gln Tyr Thr Ser Asn Tyr
690 695 700
Asn Lys Ser Val Asn Val Asp Phe Thr Val Asp Thr Asn Gly Val Tyr
705 710 715 720
Ser Glu Pro Arg Pro Ile Gly Thr Arg Tyr Leu Thr Arg Asn Leu
725 730 735
<210>134
<211>735
<212>PRT
<213>vp1,克隆hu.20
<400>134
Met Ala Ala Asp Gly Tyr Leu Pro Asp Trp Leu Glu Asp Thr Leu Ser
1 5 10 15
Glu Gly Ile Arg Gln Trp Trp Lys Leu Lys Pro Gly Pro Pro Pro Pro
20 25 30
Lys Pro Ala Glu Arg His Lys Asp Asp Ser Arg Gly Leu Val Leu Pro
35 40 45
Gly Tyr Arg Tyr Leu Gly Pro Phe Asn Gly Leu Asp Lys Gly Glu Pro
50 55 60
Val Asn Glu Ala Asp Ala Ala Ala Leu Glu His Asp Lys Ala Tyr Asp
65 70 75 80
Arg Gln Leu Asp Ser Gly Asp Asn Pro Tyr Leu Lys Tyr Asn His Val
85 90 95
Asp Ala Glu Phe Gln Glu Arg Leu Lys Glu Asp Thr Ser Phe Gly Gly
100 105 110
Asn Leu Gly Arg Ala Val Phe Gln Ala Lys Lys Arg Ile Leu Glu Pro
115 120 125
Leu Gly Leu Val Glu Glu Pro Val Lys Ala Ala Pro Gly Glu Lys Arg
130 135 140
Pro Val Glu His Ser Pro Ala Glu Pro Asp Ser Ser Ser Gly Thr Gly
145 150 155 160
Lys Ala Gly Gln Gln Pro Ala Arg Lys Arg Leu Asn Phe Gly Gln Thr
165 170 175
Gly Asp Ala Asp Ser Val Pro Asp Pro Gln Pro Leu Gly Gln Pro Pro
180 185 190
Ala Ala Pro Ser Gly Leu Gly Thr Asn Thr Met Ala Ser Gly Ser Gly
195 200 205
Ala Pro Met Ala Asp Asn Asn Glu Gly Ala Asp Gly Val Gly Asn Ser
210 215 220
Ser Gly Asn Trp His Cys Asp Ser Thr Trp Met Gly Asp Arg Val Ile
225 230 235 240
Thr Thr Ser Thr Arg Thr Trp Ala Leu Pro Thr Tyr Asn Asn His Leu
245 250 255
Tyr Lys Gln Ile Ser Ser Gln Ser Gly Ala Ser Asn Asp Asn His Tyr
260 265 270
Phe Gly Tyr Ser Thr Pro Trp Gly His Phe Asp Phe Asn Arg Phe His
275 280 285
Cys His Phe Ser Pro Arg Asp Trp Gln Arg Leu Ile Asn Asn Asn Trp
290 295 300
Gly Phe Arg Pro Lys Arg Leu Ser Phe Lys Leu Phe Asn Ile Gln Val
305 310 315 320
Lys Glu Val Thr Gln Asn Asp Gly Thr Thr Thr Ile Ala Asn Asn Leu
325 330 335
Thr Ser Thr Val Gln Val Phe Thr Asp Ser Glu Tyr Gln Leu Pro Tyr
340 345 350
Val Leu Gly Ser Ala His Gln Gly Cys Leu Pro Pro Phe Pro Ala Asp
355 360 365
Val Phe Met Val Pro Gln Tyr Gly Tyr Leu Thr Leu Asn Asn Gly Ser
370 375 380
Gln Ala Val Gly Arg Ser Ser Phe Tyr Cys Leu Glu Tyr Phe Pro Ser
385 390 395 400
Gln Met Leu Arg Thr Gly Asn Asn Phe Thr Phe Ser Tyr Thr Phe Glu
405 410 415
Asp Val Pro Phe His Ser Ser Tyr Ala His Ser Gln Ser Leu Asp Arg
420 425 430
Leu Met Asn Pro Leu Ile Asp Gln Tyr Leu Tyr Tyr Leu Ser Arg Thr
435 440 445
Asn Thr Pro Ser Gly Thr Thr Thr Met Ser Arg Leu Gln Phe Ser Gln
450 455 460
Ala Gly Ala Ser Asp Ile Arg Asp Gln Ser Arg Asn Trp Leu Pro Gly
465 470 475 480
Pro Cys Tyr Arg Gln Gln Arg Val Ser Lys Thr Ala Ala Asp Asn Asn
485 490 495
Asn Ser Asp Tyr Ser Trp Thr Gly Ala Thr Lys Tyr His Leu Asn Gly
500 505 510
Arg Asp Ser Leu Val Asn Pro Gly Pro Ala Met Ala Ser His Lys Asp
515 520 525
Asp Glu Glu Lys Tyr Phe Pro Gln Ser Gly Val Leu Ile Phe Gly Lys
530 535 540
Gln Asp Ser Gly Lys Thr Asn Val Asp Ile Glu Lys Val Met Ile Thr
545 550 555 560
Asp Glu Glu Glu Ile Arg Thr Thr Asn Pro Val Ala Thr Glu Gln Tyr
565 570 575
Gly Ser Val Ser Thr Asn Leu Gln Ser Gly Asn Thr Gln Ala Ala Thr
580 585 590
Ser Asp Val Asn Thr Gln Gly Val Leu Pro Gly Met Val Trp Gln Asp
595 600 605
Arg Asp Val Tyr Leu Gln Gly Pro Ile Trp Ala Lys Ile Pro His Thr
610 615 620
Asp Gly His Phe His Pro Ser Pro Pro Met Gly Gly Phe Gly Leu Lys
625 630 635 640
His Pro Pro Pro Gln Ile Leu Ile Lys Asn Thr Pro Val Pro Ala Asn
645 650 655
Pro Ser Thr Thr Phe Ser Ala Ala Lys Phe Ala Ser Phe Ile Thr Gln
660 665 670
Tyr Ser Thr Gly Gln Val Ser Val Glu Ile Glu Trp Glu Leu Gln Lys
675 680 685
Glu Asn Ser Lys Arg Trp Asn Pro Glu Ile Gln Tyr Thr Ser Asn Tyr
690 695 700
Asn Lys Ser Val Asn Val Asp Phe Thr Val Asp Thr Asn Gly Val Tyr
705 710 715 720
Ser Glu Pro Arg Pro Ile Gly Ala Arg Tyr Leu Thr Arg Asn Leu
725 730 735
<210>135
<211>735
<212>PRT
<213>vp1,克隆hu.21
<400>135
Met Ala Ala Asp Gly Tyr Leu Pro Asp Trp Leu Glu Asp Thr Leu Ser
1 5 10 15
Glu Gly Ile Arg Gln Trp Trp Lys Leu Lys Pro Gly Pro Pro Pro Pro
20 25 30
Lys Pro Ala Glu Arg His Lys Asp Asp Ser Arg Gly Leu Val Leu Pro
35 40 45
Gly Tyr Lys Tyr Leu Gly Pro Phe Asn Gly Leu Asp Lys Gly Glu Pro
50 55 60
Val Asn Glu Ala Asp Ala Ala Ala Leu Glu His Asp Lys Ala Tyr Asp
65 70 75 80
Arg Gln Leu Asp Ser Gly Asp Asn Pro Tyr Leu Lys Tyr Asn His Ala
85 90 95
Asp Ala Glu Phe Gln Glu Arg Leu Lys Glu Asp Thr Ser Phe Gly Gly
100 105 110
Asn Leu Gly Arg Ala Val Phe Gln Ala Lys Lys Arg Ile Leu Glu Pro
115 120 125
Leu Gly Leu Val Glu Glu Pro Val Lys Thr Ala Pro Gly Lys Lys Arg
130 135 140
Pro Val Glu His Ser Pro Ala Glu Pro Asp Ser Ser Ser Gly Thr Gly
145 150 155 160
Lys Ala Gly Gln Gln Pro Ala Arg Lys Arg Leu Asn Phe Gly Gln Thr
165 170 175
Gly Asp Ala Asp Ser Val Pro Asp Pro Arg Pro Leu Gly Gln Pro Pro
180 185 190
Ala Ala Pro Ser Gly Leu Gly Thr Asn Thr Met Ala Ser Gly Ser Gly
195 200 205
Ala Pro Met Ala Asp Asn Asn Glu Gly Ala Asp Gly Val Gly Asn Ser
210 215 220
Ser Gly Asn Trp His Cys Asp Ser Thr Trp Met Gly Asp Arg Val Ile
225 230 235 240
Thr Thr Ser Thr Arg Thr Trp Ala Leu Pro Thr Tyr Asn Asn His Leu
245 250 255
Tyr Lys Gln Ile Ser Ser Gln Ser Gly Ala Ser Asn Asp Asn His Tyr
260 265 270
Phe Gly Tyr Ser Thr Pro Trp Gly Tyr Phe Asp Phe Asn Arg Phe His
275 280 285
Cys His Phe Ser Pro Arg Asp Trp Gln Arg Leu Ile Asn Asn Asn Trp
290 295 300
Gly Phe Arg Pro Lys Arg Leu Ser Phe Lys Leu Phe Asn Ile Gln Val
305 310 315 320
Lys Glu Val Thr Gln Asn Asp Gly Thr Thr Thr Ile Ala Asn Asn Leu
325 330 335
Thr Ser Thr Val Gln Val Phe Thr Asp Ser Glu Tyr Gln Leu Pro Tyr
340 345 350
Val Leu Gly Ser Ala His Gln Gly Cys Leu Pro Pro Phe Pro Ala Asp
355 360 365
Val Phe Met Val Pro Gln Tyr Gly Tyr Leu Thr Leu Asn Asn Gly Ser
370 375 380
Gln Ala Val Gly Arg Ser Ser Phe Tyr Cys Leu Glu Tyr Phe Pro Ser
385 390 395 400
Gln Met Leu Arg Thr Gly Asn Asn Phe Thr Phe Ser Tyr Thr Phe Glu
405 410 415
Asp Val Pro Phe His Ser Ser Tyr Ala His Ser Gln Ser Leu Asp Arg
420 425 430
Leu Met Asn Pro Leu Ile Asp Gln Tyr Leu Tyr Tyr Leu Ser Arg Thr
435 440 445
Asn Thr Pro Ser Gly Thr Thr Thr Met Ser Arg Leu Gln Phe Ser Gln
450 455 460
Ala Gly Ala Ser Asp Ile Arg Asp Gln Ser Arg Asn Trp Leu Pro Gly
465 470 475 480
Pro Cys Tyr Arg Gln Gln Arg Val Ser Lys Thr Ala Ala Asp Asn Asn
485 490 495
Asn Ser Asp Tyr Ser Trp Thr Gly Ala Thr Lys Tyr His Leu Asn Gly
500 505 510
Arg Asp Ser Leu Val Asn Pro Gly Pro Ala Met Ala Ser His Lys Asp
515 520 525
Asp Glu Glu Lys Tyr Phe Pro Gln Ser Gly Val Leu Ile Phe Gly Lys
530 535 540
Gln Asp Ser Gly Lys Thr Asn Val Asp Ile Glu Lys Val Met Ile Thr
545 550 555 560
Asp Glu Glu Glu Ile Arg Thr Thr Asn Pro Val Ala Thr Glu Gln Tyr
565 570 575
Gly Ser Val Ser Thr Asn Leu Gln Ser Gly Asn Thr Gln Ala Ala Thr
580 585 590
Ser Asp Val Asn Thr Gln Gly Val Leu Pro Gly Met Val Trp Gln Asp
595 600 605
Arg Asp Val Tyr Leu Gln Gly Pro Ile Trp Ala Lys Ile Pro His Thr
610 615 620
Asp Gly His Phe His Pro Ser Pro Leu Met Gly Gly Phe Gly Leu Lys
625 630 635 640
His Pro Pro Pro Gln Ile Leu Ile Lys Asn Thr Pro Val Pro Ala Asn
645 650 655
Pro Ser Thr Thr Phe Ser Ala Ala Lys Phe Ala Ser Phe Ile Thr Gln
660 665 670
Tyr Ser Thr Gly Gln Val Ser Val Glu Ile Glu Trp Glu Leu Gln Lys
675 680 685
Glu Asn Ser Lys Arg Trp Asn Pro Glu Ile Gln Tyr Thr Ser Asn Tyr
690 695 700
Asn Lys Ser Val Asn Val Asp Phe Thr Val Asp Thr Asn Gly Val Tyr
705 710 715 720
Ser Glu Pro Arg Pro Ile Gly Thr Arg Tyr Leu Thr Arg Asn Leu
725 730 735
<210>136
<211>735
<212>PRT
<213>vp1,克隆hu.24
<400>136
Met Ala Ala Asp Gly Tyr Leu Pro Asp Trp Leu Glu Asp Thr Leu Ser
1 5 10 15
Glu Gly Ile Arg Gln Trp Trp Lys Leu Lys Pro Gly Pro Pro Pro Pro
20 25 30
Lys Pro Ala Glu Arg His Lys Asp Asp Ser Arg Gly Leu Val Leu Pro
35 40 45
Gly Tyr Lys Tyr Leu Gly Pro Phe Asn Gly Leu Asp Lys Gly Glu Pro
50 55 60
Val Asn Glu Ala Asp Ala Ala Ala Leu Glu His Asp Lys Ala Tyr Asp
65 70 75 80
Arg Gln Leu Asp Ser Gly Asp Asn Pro Tyr Leu Lys Tyr Asn His Ala
85 90 95
Asp Ala Glu Phe Gln Glu Arg Leu Lys Glu Asp Thr Ser Phe Gly Gly
100 105 110
Asn Leu Gly Arg Ala Val Phe Gln Ala Lys Lys Arg Ile Leu Glu Pro
115 120 125
Leu Gly Leu Val Glu Glu Pro Val Lys Thr Ala Pro Gly Lys Lys Arg
130 135 140
Pro Val Glu His Ser Pro Ala Glu Pro Asp Ser Ser Ser Gly Thr Gly
145 150 155 160
Lys Ala Gly Gln Gln Pro Ala Arg Lys Arg Leu Asn Phe Gly Gln Thr
165 170 175
Gly Asp Ala Asp Ser Val Pro Asp Pro Arg Pro Leu Gly Gln Pro Pro
180 185 190
Ala Ala Pro Ser Gly Leu Gly Thr Asn Thr Met Ala Ser Gly Ser Gly
195 200 205
Ala Pro Met Ala Asp Asn Asn Glu Gly Ala Asp Gly Val Gly Asn Ser
210 215 220
Ser Gly Asn Trp His Cys Asp Ser Thr Trp Met Gly Asp Arg Val Ile
225 230 235 240
Thr Thr Ser Thr Arg Thr Trp Ala Leu Pro Thr Tyr Asn Asn His Leu
245 250 255
Tyr Lys Gln Ile Ser Ser Gln Ser Gly Ala Ser Asn Asp Asn His Tyr
260 265 270
Phe Gly Tyr Ser Thr Pro Trp Gly Tyr Phe Asp Phe Asn Arg Phe His
275 280 285
Cys His Phe Ser Pro Arg Asp Trp Gln Arg Leu Ile Asn Asn Asn Trp
290 295 300
Gly Phe Arg Pro Lys Arg Leu Ser Phe Lys Leu Phe Asn Ile Gln Val
305 310 315 320
Lys Glu Val Thr Gln Asn Asp Gly Thr Thr Thr Ile Ala Asn Asn Leu
325 330 335
Thr Ser Thr Val Gln Val Phe Thr Asp Ser Glu Tyr Gln Leu Pro Tyr
340 345 350
Val Leu Gly Ser Ala His Gln Gly Cys Leu Pro Pro Phe Pro Ala Asp
355 360 365
Val Phe Met Val Pro Gln Tyr Gly Tyr Leu Thr Leu Asn Asn Gly Ser
370 375 380
Gln Ala Val Gly Arg Ser Ser Phe Tyr Cys Leu Glu Tyr Phe Pro Ser
385 390 395 400
Gln Met Leu Arg Thr Gly Asn Asn Phe Thr Phe Ser Tyr Thr Phe Glu
405 410 415
Asp Val Pro Phe His Ser Ser Tyr Ala His Ser Gln Ser Leu Asp Arg
420 425 430
Leu Met Asn Pro Leu Ile Asp Gln Tyr Leu Tyr Tyr Leu Ser Arg Thr
435 440 445
Asn Thr Pro Ser Gly Thr Thr Thr Met Ser Arg Leu Gln Phe Ser Gln
450 455 460
Ala Gly Ala Ser Asp Ile Arg Asp Gln Ser Arg Asn Trp Leu Pro Gly
465 470 475 480
Pro Cys Tyr Arg Gln Gln Arg Val Ser Lys Thr Ala Ala Asp Asn Asn
485 490 495
Asn Ser Asp Tyr Ser Trp Thr Gly Ala Thr Lys Tyr His Leu Asn Gly
500 505 510
Arg Asp Ser Leu Val Asn Pro Gly Pro Ala Met Ala Ser His Lys Asp
515 520 525
Asp Glu Glu Lys Tyr Phe Pro Gln Ser Gly Val Leu Ile Phe Gly Lys
530 535 540
Gln Asp Ser Gly Lys Thr Asn Val Asp Ile Glu Lys Val Met Ile Thr
545 550 555 560
Asp Glu Glu Glu Ile Arg Thr Thr Asn Pro Val Ala Thr Glu Gln Tyr
565 570 575
Gly Ser Val Ser Thr Asn Leu Gln Ser Gly Asn Thr Gln Ala Ala Thr
580 585 590
Ser Asp Val Asn Thr Gln Gly Val Leu Pro Gly Met Val Trp Gln Asp
595 600 605
Arg Asp Val Tyr Leu Gln Gly Pro Ile Trp Ala Lys Ile Pro His Thr
610 615 620
Asp Gly His Phe His Pro Ser Pro Leu Met Gly Gly Phe Gly Leu Lys
625 630 635 640
His Pro Pro Pro Gln Ile Leu Ile Lys Asn Thr Pro Val Pro Ala Asn
645 650 655
Pro Ser Thr Thr Phe Ser Ala Ala Lys Phe Ala Ser Phe Ile Thr Gln
660 665 670
Tyr Ser Thr Gly Gln Val Ser Val Glu Ile Glu Trp Glu Leu Gln Lys
675 680 685
Glu Asn Ser Lys Arg Trp Asn Pro Glu Ile Gln Tyr Thr Ser Asn Tyr
690 695 700
Asn Lys Ser Val Asn Val Asp Phe Thr Val Asp Thr Asn Gly Val Tyr
705 710 715 720
Ser Glu Pro Arg Pro Ile Gly Thr Arg Tyr Leu Thr Arg Asn Leu
725 730 735
<210>137
<211>735
<212>PRT
<213>vp1,克隆hu.23-2
<400>137
Met Ala Ala Asp Gly Tyr Leu Pro Asp Trp Leu Glu Asp Thr Leu Ser
1 5 10 15
Glu Gly Ile Arg Gln Trp Trp Lys Leu Lys Pro Gly Pro Pro Pro Pro
20 25 30
Lys Pro Ala Glu Arg His Lys Asp Asp Ser Arg Gly Leu Val Leu Pro
35 40 45
Gly Tyr Lys Tyr Leu Gly Pro Phe Asn Gly Leu Asp Lys Gly Glu Pro
50 55 60
Val Asn Glu Ala Asp Ala Ala Ala Leu Glu His Asp Lys Ala Tyr Asp
65 70 75 80
Arg Gln Leu Asp Ser Gly Asp Asn Pro Tyr Leu Lys Tyr Asn His Ala
85 90 95
Asp Ala Glu Phe Gln Glu Arg Leu Lys Glu Asp Thr Ser Phe Gly Gly
100 105 110
Asn Leu Gly Arg Ala Val Phe Gln Ala Lys Lys Arg Ile Leu Glu Pro
115 120 125
Leu Gly Leu Val Glu Glu Pro Val Lys Thr Ala Pro Gly Lys Lys Arg
130 135 140
Pro Val Glu His Ser Pro Ala Glu Pro Asp Ser Ser Ser Gly Thr Gly
145 150 155 160
Lys Ala Gly Gln Gln Pro Ala Arg Lys Arg Leu Asn Phe Gly Gln Thr
165 170 175
Gly Asp Ala Asp Ser Val Pro Asp Pro Gln Pro Leu Gly Gln Pro Pro
180 185 190
Ala Ala Pro Ser Gly Leu Gly Thr Asn Thr Met Ala Ser Gly Ser Gly
195 200 205
Ala Pro Met Ala Asp Asn Asn Glu Gly Ala Asp Gly Val Gly Asn Ser
210 215 220
Ser Gly Asn Trp His Cys Asp Ser Thr Trp Met Gly Asp Arg Val Ile
225 230 235 240
Thr Thr Ser Thr Arg Thr Trp Ala Leu Pro Thr Cys Asn Asn His Leu
245 250 255
Tyr Lys Gln Ile Ser Ser Gln Ser Gly Ala Ser Asn Asp Asn His Tyr
260 265 270
Phe Gly Tyr Ser Thr Pro Trp Gly Tyr Phe Asp Phe Asn Arg Phe His
275 280 285
Cys His Phe Ser Pro Arg Asp Trp Gln Arg Leu Ile Asn Asn Asn Trp
290 295 300
Gly Phe Arg Pro Lys Arg Leu Ser Phe Lys Leu Phe Asn Ile Gln Val
305 310 315 320
Lys Glu Val Thr Gln Asn Asp Gly Thr Thr Thr Ile Ala Asn Asn Leu
325 330 335
Thr Ser Thr Val Gln Val Phe Thr Asp Ser Glu Tyr Gln Leu Pro Tyr
340 345 350
Val Leu Gly Ser Ala His Gln Gly Cys Leu Pro Pro Phe Pro Ala Asp
355 360 365
Val Phe Met Val Pro Gln Tyr Gly Tyr Leu Thr Leu Asn Asn Gly Ser
370 375 380
Gln Ala Val Gly Arg Ser Ser Phe Tyr Cys Leu Glu Tyr Phe Pro Ser
385 390 395 400
Gln Met Leu Arg Thr Gly Asn Asn Phe Thr Phe Ser Tyr Thr Phe Glu
405 410 415
Asp Val Pro Phe His Ser Ser Tyr Ala His Ser Gln Ser Leu Asp Arg
420 425 430
Leu Met Asn Pro Leu Ile Asp Gln Tyr Leu Tyr Tyr Leu Ser Arg Thr
435 440 445
Asn Thr Pro Ser Gly Thr Thr Thr Met Ser Arg Leu Gln Phe Ser Gln
450 455 460
Ala Gly Ala Ser Asp Ile Arg Asp Gln Ser Arg Asn Trp Leu Pro Gly
465 470 475 480
Pro Cys Tyr Arg Gln Gln Arg Val Ser Lys Thr Ala Ala Asp Asn Asn
485 490 495
Asn Ser Asp Tyr Ser Trp Thr Gly Ala Thr Lys Tyr His Leu Asn Gly
500 505 510
Arg Asp Ser Leu Val Asn Pro Gly Pro Ala Met Ala Ser His Lys Asp
515 520 525
Asp Glu Glu Lys Tyr Phe Pro Gln Ser Gly Val Leu Ile Phe Gly Lys
530 535 540
Gln Asp Ser Gly Lys Thr Asn Val Asp Ile Glu Lys Val Met Ile Thr
545 550 555 560
Asp Glu Glu Glu Ile Arg Thr Thr Asn Pro Val Ala Thr Glu Gln Tyr
565 570 575
Gly Ser Val Ser Thr Tyr Leu Gln Ser Gly Asn Thr Gln Ala Ala Thr
580 585 590
Ser Asp Val Asn Thr Gln Gly Val Leu Pro Gly Met Val Trp Gln Asp
595 600 605
Arg Asp Val Tyr Leu Arg Gly Pro Ile Trp Ala Lys Ile Pro His Thr
610 615 620
Asp Gly His Phe His Pro Ser Pro Leu Met Gly Gly Phe Gly Leu Lys
625 630 635 640
His Pro Pro Pro Gln Ile Leu Ile Lys Asn Thr Pro Val Pro Ala Asn
645 650 655
Pro Ser Thr Thr Phe Ser Ala Ala Lys Phe Ala Ser Phe Ile Thr Gln
660 665 670
Tyr Ser Thr Gly Gln Val Ser Val Glu Ile Glu Trp Glu Leu Gln Lys
675 680 685
Glu Asn Ser Lys Arg Trp Asn Pro Glu Ile Gln Tyr Thr Ser Asn Tyr
690 695 700
Asn Lys Ser Val Asn Val Asp Phe Thr Val Asp Thr Asn Gly Val Tyr
705 710 715 720
Ser Glu Pro Arg Pro Ile Gly Thr Arg Tyr Leu Thr Arg Asn Leu
725 730 735
<210>138
<211>735
<212>PRT
<213>vp1,克隆hu.22
<400>138
Met Ala Ala Asp Gly Tyr Leu Pro Asp Trp Leu Glu Asp Thr Leu Ser
1 5 10 15
Glu Gly Ile Arg Gln Trp Trp Lys Leu Lys Pro Gly Pro Pro Pro Pro
20 25 30
Lys Pro Ala Glu Arg His Lys Asp Asp Ser Arg Gly Leu Val Leu Pro
35 40 45
Gly Tyr Lys Tyr Leu Gly Pro Phe Asn Gly Leu Asp Lys Gly Glu Pro
50 55 60
Val Asn Glu Ala Asp Ala Ala Ala Leu Glu His Asp Lys Ala Tyr Asp
65 70 75 80
Arg Gln Leu Asp Ser Gly Asp Asn Pro Tyr Leu Lys Tyr Asn His Ala
85 90 95
Asp Ala Glu Phe Gln Glu Arg Leu Lys Gly Asp Thr Ser Phe Gly Gly
100 105 110
Asn Leu Gly Arg Ala Val Phe Gln Ala Lys Lys Arg Ile Leu Glu Pro
115 120 125
Leu Gly Leu Val Glu Glu Pro Val Lys Thr Ala Pro Gly Lys Lys Arg
130 135 140
Pro Val Glu His Ser Pro Ala Glu Pro Asp Ser Ser Ser Gly Thr Gly
145 150 155 160
Lys Ala Gly Gln Gln Pro Ala Arg Lys Arg Leu Asn Phe Gly Gln Thr
165 170 175
Gly Asp Ala Asp Ser Val Pro Asp Pro Gln Pro Leu Gly Gln Pro Pro
180 185 190
Ala Ala Pro Ser Gly Leu Gly Thr Asn Thr Met Ala Ser Gly Ser Gly
195 200 205
Ala Pro Met Ala Asp Asn Asn Glu Gly Ala Asp Gly Val Gly Asn Ser
210 215 220
Ser Gly Asn Trp His Cys Asp Ser Thr Trp Met Gly Gly Arg Val Ile
225 230 235 240
Thr Thr Ser Thr Arg Thr Trp Ala Leu Pro Thr Tyr Asn Asn His Leu
245 250 255
Tyr Lys Gln Ile Ser Ser Gln Ser Gly Ala Ser Asn Asp Asn His Tyr
260 265 270
Phe Gly Tyr Ser Thr Pro Trp Gly Tyr Phe Asp Phe Asn Arg Phe His
275 280 285
Cys His Phe Ser Pro Arg Asp Trp Gln Arg Leu Ile Asn Asn Asn Trp
290 295 300
Gly Phe Arg Pro Lys Arg Leu Ser Phe Lys Leu Phe Asn Ile Gln Val
305 310 315 320
Lys Glu Val Thr Gln Asn Asp Gly Thr Thr Thr Ile Ala Asn Asn Leu
325 330 335
Thr Ser Thr Val Gln Val Phe Thr Asp Ser Glu Tyr Gln Leu Pro Tyr
340 345 350
Val Leu Gly Ser Ala His Gln Gly Cys Leu Pro Pro Phe Pro Ala Asp
355 360 365
Val Phe Met Val Pro Gln Tyr Gly Tyr Leu Thr Leu Asn Asn Gly Ser
370 375 380
Gln Ala Val Gly Arg Ser Ser Phe Tyr Cys Leu Glu Tyr Phe Pro Ser
385 390 395 400
Gln Thr Leu Arg Thr Gly Asn Asn Phe Thr Phe Ser Tyr Thr Phe Glu
405 410 415
Asp Val Pro Phe His Ser Ser Tyr Ala His Ser Gln Ser Leu Asp Arg
420 425 430
Leu Met Asn Pro Leu Ile Asp Gln Tyr Leu Tyr Tyr Leu Ser Arg Thr
435 440 445
Asn Thr Pro Ser Gly Thr Thr Thr Met Ser Arg Leu Gln Phe Ser Gln
450 455 460
Ala Gly Ala Ser Asp Ile Arg Asp Gln Ser Arg Asn Trp Leu Pro Gly
465 470 475 480
Pro Cys Tyr Arg Gln Gln Arg Val Ser Lys Thr Ala Ala Asp Asn Asn
485 490 495
Asn Ser Asp Tyr Ser Trp Thr Gly Ala Thr Lys Tyr His Leu Asn Gly
500 505 510
Arg Asp Ser Leu Val Asn Pro Gly Pro Ala Met Ala Ser His Lys Asp
515 520 525
Asp Glu Glu Lys Tyr Phe Pro Gln Ser Gly Val Leu Ile Phe Gly Lys
530 535 540
Gln Asp Ser Gly Lys Thr Asn Val Asp Ile Glu Lys Val Met Ile Thr
545 550 555 560
Asp Glu Glu Glu Ile Arg Thr Thr Asn Pro Val Ala Thr Glu Gln Tyr
565 570 575
Gly Ser Val Ser Thr Asn Leu Gln Ser Gly Asn Thr Gln Ala Ala Thr
580 585 590
Ser Asp Val Asn Thr Gln Gly Val Leu Pro Gly Met Val Trp Gln Asp
595 600 605
Arg Asp Val Tyr Leu Gln Gly Pro Ile Trp Ala Lys Ile Pro His Thr
610 615 620
Asp Gly His Phe His Pro Ser Pro Leu Met Gly Gly Phe Gly Leu Lys
625 630 635 640
His Pro Pro Pro Gln Ile Leu Ile Lys Asn Thr Pro Val Pro Ala Asn
645 650 655
Pro Ser Thr Thr Phe Ser Ala Ala Lys Phe Ala Ser Phe Ile Thr Gln
660 665 670
Tyr Ser Thr Gly Gln Val Ser Val Glu Ile Glu Trp Glu Leu Gln Lys
675 680 685
Glu Asn Ser Lys Arg Trp Asn Pro Glu Ile Gln Tyr Thr Ser Asn Tyr
690 695 700
Asn Lys Ser Val Asn Val Asp Phe Thr Val Asp Thr Asn Gly Val Tyr
705 710 715 720
Ser Glu Pro Arg Pro Ile Gly Thr Arg Tyr Leu Thr Arg Asn Leu
725 730 735
<210>139
<211>735
<212>PRT
<213>vp1,克隆hu.26
<400>139
Met Ala Ala Asp Gly Tyr Leu Pro Asp Trp Leu Glu Asp Thr Leu Ser
1 5 10 15
Glu Gly Ile Arg Gln Trp Trp Lys Leu Lys Pro Gly Pro Pro Pro Pro
20 25 30
Lys Pro Ala Glu Arg His Lys Asp Asp Ser Arg Gly Leu Val Leu Pro
35 40 45
Gly Tyr Lys Tyr Leu Gly Pro Phe Asn Gly Leu Asp Lys Gly Glu Pro
50 55 60
Val Asn Glu Ala Asp Ala Ala Ala Leu Glu His Asp Lys Ala Tyr Asp
65 70 75 80
Arg Gln Leu Asp Ser Gly Asp Asn Pro Tyr Leu Lys Tyr Asn His Ala
85 90 95
Asp Ala Glu Phe Gln Glu Arg Leu Lys Glu Asp Thr Ser Phe Gly Gly
100 105 110
Asn Leu Gly Arg Ala Val Phe Gln Ala Lys Lys Arg Ile Leu Glu Pro
115 120 125
Leu Gly Leu Val Glu Glu Pro Val Lys Thr Ala Pro Gly Lys Lys Arg
130 135 140
Pro Val Glu His Ser Pro Ala Glu Pro Asp Ser Ser Ser Gly Thr Gly
145 150 155 160
Lys Ala Gly Gln Gln Pro Ala Arg Lys Arg Leu Asn Phe Gly Gln Thr
165 170 175
Gly Asp Ala Asp Ser Val Pro Asp Pro Gln Pro Leu Gly Gln Pro Pro
180 185 190
Ala Ala Pro Ser Gly Leu Gly Thr Asn Thr Met Ala Ser Gly Ser Gly
195 200 205
Ala Pro Met Ala Asp Asn Asn Glu Gly Ala Asp Gly Val Gly Asn Ser
210 215 220
Ser Gly Asn Trp His Cys Asp Ser Thr Trp Met Gly Asp Arg Val Ile
225 230 235 240
Thr Thr Ser Thr Arg Thr Trp Ala Leu Pro Thr Tyr Asn Asn His Leu
245 250 255
Tyr Lys Gln Ile Ser Ser Gln Ser Gly Ala Ser Asn Asp Asn His Tyr
260 265 270
Phe Gly Tyr Ser Thr Pro Trp Gly Tyr Phe Asp Phe Asn Arg Phe His
275 280 285
Cys His Phe Ser Pro Arg Asp Trp Gln Arg Leu Ile Asn Asn Asn Trp
290 295 300
Gly Phe Arg Pro Lys Arg Leu Ser Phe Lys Leu Phe Asn Ile Gln Val
305 310 315 320
Lys Glu Val Thr Gln Asn Asp Gly Thr Thr Thr Ile Ala Asn Asn Leu
325 330 335
Thr Ser Thr Val Gln Val Phe Thr Asp Ser Glu Tyr Gln Leu Pro Tyr
340 345 350
Val Leu Gly Ser Ala His Gln Gly Cys Leu Pro Pro Phe Pro Ala Asp
355 360 365
Val Phe Met Val Pro Gln Tyr Gly Tyr Leu Thr Leu Asn Asn Gly Ser
370 375 380
Gln Ala Val Gly Arg Ser Ser Phe Tyr Cys Leu Glu Tyr Phe Pro Ser
385 390 395 400
Gln Met Leu Arg Thr Gly Asn Asn Phe Thr Phe Ser Tyr Thr Phe Glu
405 410 415
Asp Val Pro Phe His Ser Ser Tyr Ala His Ser Gln Ser Leu Asp Arg
420 425 430
Leu Met Asn Pro Leu Ile Asp Gln Tyr Leu Tyr Tyr Leu Ser Arg Thr
435 440 445
Asn Thr Pro Ser Gly Thr Thr Thr Met Ser Arg Leu Gln Phe Ser Gln
450 455 460
Ala Gly Ala Ser Asp Ile Arg Asp Gln Ser Arg Asn Trp Leu Pro Gly
465 470 475 480
Pro Cys Tyr Arg Gln Gln Arg Val Ser Lys Thr Ala Ala Asp Asn Asn
485 490 495
Asn Ser Asp Tyr Ser Trp Thr Gly Ala Thr Lys Tyr His Leu Asn Gly
500 505 510
Arg Asp Ser Leu Val Asn Pro Gly Pro Ala Met Ala Ser His Lys Asp
515 520 525
Asp Glu Glu Lys Tyr Phe Pro Gln Ser Gly Val Leu Ile Phe Gly Lys
530 535 540
Gln Asp Ser Gly Lys Thr Asn Val Asp Ile Glu Lys Val Met Ile Thr
545 550 555 560
Asp Glu Glu Glu Ile Arg Thr Thr Asn Pro Val Ala Thr Glu Gln Tyr
565 570 575
Gly Ser Val Ser Thr Asn Leu Gln Ser Gly Asn Thr Gln Ala Ala Thr
580 585 590
Ser Asp Val Asn Thr Gln Gly Val Leu Pro Gly Met Val Trp Gln Asp
595 600 605
Arg Asp Val Tyr Leu Gln Gly Pro Ile Trp Ala Lys Ile Pro His Thr
610 615 620
Asp Gly His Phe His Pro Ser Pro Leu Met Gly Gly Phe Gly Leu Lys
625 630 635 640
His Pro Pro Pro Gln Ile Leu Ile Lys Asn Thr Pro Val Pro Ala Asn
645 650 655
Pro Ser Thr Thr Phe Ser Ala Ala Lys Phe Ala Ser Phe Ile Thr Gln
660 665 670
Tyr Ser Thr Gly Gln Val Ser Val Glu Ile Glu Trp Glu Leu Gln Lys
675 680 685
Glu Asn Ser Lys Arg Trp Asn Pro Glu Ile Gln Tyr Thr Ser Asn Tyr
690 695 700
Asn Lys Ser Val Asn Val Asp Phe Thr Val Asp Thr Asn Gly Val Tyr
705 710 715 720
Ser Glu Pro Arg Pro Ile Gly Thr Arg Tyr Leu Thr Arg Asn Leu
725 730 735
<210>140
<211>735
<212>PRT
<213>vp1,克隆hu.27
<400>140
Met Ala Ala Asp Gly Tyr Leu Pro Asp Trp Leu Glu Asp Thr Leu Ser
1 5 10 15
Glu Gly Ile Arg Gln Trp Trp Lys Leu Lys Pro Gly Pro Pro Pro Pro
20 25 30
Lys Pro Ala Glu Arg His Lys Asp Asp Ser Arg Gly Leu Val Leu Pro
35 40 45
Gly Tyr Lys Tyr Leu Gly Pro Phe Asn Gly Leu Asp Lys Gly Glu Pro
50 55 60
Val Asn Glu Ala Asp Ala Ala Ala Leu Glu His Asp Lys Ala Tyr Asp
65 70 75 80
Arg Gln Leu Asp Ser Gly Asp Asn Pro Tyr Leu Lys Tyr Asn His Ala
85 90 95
Asp Ala Glu Phe Gln Glu Arg Leu Lys Glu Asp Thr Ser Phe Gly Gly
100 105 110
Asn Leu Gly Arg Ala Val Phe Gln Ala Lys Lys Arg Ile Leu Glu Pro
115 120 125
Leu Gly Leu Val Glu Glu Pro Val Lys Thr Ala Pro Gly Lys Lys Arg
130 135 140
Pro Val Glu His Ser Pro Ala Glu Pro Asp Ser Ser Ser Gly Thr Gly
145 150 155 160
Lys Ala Gly Gln Gln Pro Ala Arg Lys Arg Leu Asn Phe Gly Gln Thr
165 170 175
Gly Asp Ala Asp Ser Val Pro Asp Pro Gln Pro Leu Gly Gln Pro Pro
180 185 190
Ala Ala Pro Ser Gly Leu Gly Thr Asn Thr Met Ala Ser Gly Ser Gly
195 200 205
Ala Pro Met Ala Asp Asn Asn Glu Gly Ala Asp Gly Val Gly Asn Ser
210 215 220
Ser Gly Asn Trp His Cys Asp Ser Thr Trp Met Gly Asp Arg Val Ile
225 230 235 240
Thr Thr Ser Thr Arg Thr Trp Ala Leu Pro Thr Tyr Asn Asn His Leu
245 250 255
Tyr Lys Gln Ile Ser Ser Gln Ser Gly Ala Ser Asn Asp Asn His Tyr
260 265 270
Phe Gly Tyr Ser Thr Pro Trp Gly Tyr Phe Asp Phe Asn Arg Phe His
275 280 285
Cys His Phe Ser Pro Arg Asp Trp Gln Arg Leu Ile Asn Asn Asn Trp
290 295 300
Gly Phe Arg Pro Lys Arg Leu Ser Phe Lys Leu Phe Asn Ile Gln Val
305 310 315 320
Lys Glu Val Thr Gln Asn Asp Gly Thr Thr Thr Ile Ala Asn Asn Leu
325 330 335
Thr Ser Thr Val Gln Val Phe Thr Asp Ser Gly Tyr Gln Leu Pro Tyr
340 345 350
Val Leu Gly Ser Ala His Gln Gly Cys Leu Pro Pro Phe Pro Ala Asp
355 360 365
Val Phe Met Val Pro Gln Tyr Gly Tyr Leu Thr Leu Asn Asn Gly Ser
370 375 380
Gln Ala Val Gly Arg Ser Ser Phe Tyr Cys Leu Glu Tyr Phe Pro Ser
385 390 395 400
Gln Met Leu Arg Thr Gly Asn Asn Phe Thr Phe Ser Tyr Thr Phe Glu
405 410 415
Asp Val Pro Phe His Ser Ser Tyr Ala His Gly Gln Ser Leu Asp Arg
420 425 430
Leu Met Asn Pro Leu Ile Asp Gln Tyr Leu Tyr Tyr Leu Ser Arg Thr
435 440 445
Asn Thr Pro Ser Gly Thr Thr Thr Met Ser Arg Leu Gln Phe Ser Gln
450 455 460
Ala Gly Ala Ser Asp Val Arg Asp Gln Ser Arg Asn Trp Leu Pro Gly
465 470 475 480
Pro Cys Tyr Arg Gln Gln Arg Val Ser Lys Thr Ala Ala Asp Asn Asn
485 490 495
Asn Ser Asp Tyr Ser Trp Thr Gly Ala Thr Lys Tyr His Leu Asn Gly
500 505 510
Arg Asp Ser Leu Val Asn Pro Gly Pro Ala Met Ala Ser His Lys Asp
515 520 525
Asp Glu Glu Lys Tyr Phe Pro Gln Ser Gly Val Leu Val Phe Gly Lys
530 535 540
Gln Asp Ser Gly Lys Thr Asn Val Asp Ile Glu Lys Val Met Ile Thr
545 550 555 560
Asp Glu Glu Glu Ile Arg Thr Thr Asn Pro Ala Ala Thr Glu Gln Tyr
565 570 575
Gly Ser Val Ser Thr Asn Leu Gln Ser Gly Asn Thr Gln Ala Ala Thr
580 585 590
Ser Asp Val Asn Thr Gln Gly Val Leu Pro Gly Met Val Trp Gln Asp
595 600 605
Arg Asp Val Tyr Leu Gln Gly Pro Ile Trp Ala Lys Ile Pro His Thr
610 615 620
Asp Gly His Phe His Pro Ser Pro Leu Met Gly Gly Phe Gly Leu Lys
625 630 635 640
His Pro Pro Pro Gln Ile Leu Ile Lys Asn Thr Pro Val Pro Ala Asn
645 650 655
Pro Ser Thr Thr Phe Ser Ala Ala Lys Phe Val Ser Phe Ile Thr Gln
660 665 670
Tyr Ser Thr Gly Gln Val Ser Val Glu Ile Glu Trp Glu Leu Gln Lys
675 680 685
Glu Asn Ser Lys Arg Trp Asn Pro Glu Ile Gln Tyr Thr Ser Asn Tyr
690 695 700
Asn Lys Ser Val Asn Val Asp Phe Thr Val Asp Thr Asn Gly Val Tyr
705 710 715 720
Ser Glu Pro Arg Pro Ile Gly Thr Arg Tyr Leu Thr Arg Asn Leu
725 730 735
<210>141
<211>735
<212>PRT
<213>vp1,克隆hu.4
<400>141
Met Ala Ala Asp Gly Tyr Leu Pro Asp Trp Leu Glu Asp Thr Leu Ser
1 5 10 15
Glu Gly Ile Arg Gln Trp Trp Lys Leu Lys Pro Gly Pro Pro Pro Pro
20 25 30
Lys Pro Ala Glu Arg His Lys Asp Asp Ser Arg Gly Leu Val Leu Pro
35 40 45
Gly Tyr Lys Tyr Leu Gly Pro Phe Asn Gly Leu Asp Lys Gly Glu Pro
50 55 60
Val Asn Glu Ala Asp Ala Ala Ala Leu Glu His Asp Lys Ala Tyr Asp
65 70 75 80
Arg Gln Leu Asp Ser Gly Asp Asn Pro Tyr Leu Lys Tyr Asn His Ala
85 90 95
Asp Ala Glu Phe Gln Glu Arg Leu Lys Glu Asp Thr Ser Phe Gly Gly
100 105 110
Asn Leu Gly Arg Ala Val Phe Gln Ala Lys Lys Arg Val Leu Glu Pro
115 120 125
Leu Gly Leu Val Glu Glu Pro Val Lys Thr Ala Pro Gly Lys Lys Arg
130 135 140
Pro Val Glu His Ser Pro Val Glu Pro Asp Ser Ser Ser Gly Thr Gly
145 150 155 160
Lys Ala Gly Gln Gln Pro Ala Arg Lys Arg Leu Asn Phe Gly Gln Thr
165 170 175
Gly Asp Ala Asp Ser Val Pro Asp Pro Gln Pro Leu Gly Gln Pro Pro
180 185 190
Ala Ala Pro Ser Gly Leu Gly Ser Thr Thr Met Ala Thr Gly Ser Gly
195 200 205
Ala Pro Met Ala Asp Asn Asn Glu Gly Ala Asp Gly Val Gly Asn Ser
210 215 220
Ser Gly Asn Trp His Cys Asp Ser Gln Trp Leu Gly Asp Arg Val Ile
225 230 235 240
Thr Thr Ser Thr Arg Thr Trp Ala Leu Pro Thr Tyr Asn Asn His Leu
245 250 255
Tyr Lys Gln Ile Ser Ser Gln Ser Gly Ala Ser Asn Asp Asn His Tyr
260 265 270
Phe Gly Tyr Ser Thr Pro Trp Gly Tyr Phe Asp Phe Asn Arg Phe His
275 280 285
Cys His Phe Ser Pro Arg Asp Trp Gln Arg Leu Val Asn Asn Asn Arg
290 295 300
Gly Phe Arg Pro Lys Arg Leu Asn Phe Lys Leu Phe Asn Ile Gln Val
305 310 315 320
Lys Glu Val Thr Gln Asn Asp Gly Thr Thr Thr Ile Ala Asn Asn Leu
325 330 335
Thr Ser Thr Val Gln Val Phe Thr Asp Ser Glu Tyr Gln Leu Pro Tyr
340 345 350
Val Leu Gly Ser Ala His Gln Gly Cys Leu Pro Pro Phe Pro Ala Asp
355 360 365
Val Phe Met Val Pro Gln Tyr Gly Tyr Leu Thr Leu Asn Asn Gly Ser
370 375 380
Gln Ala Val Gly Arg Ser Ser Phe Tyr Cys Leu Glu Tyr Phe Pro Ser
385 390 395 400
Gln Met Leu Arg Thr Gly Asn Asn Phe Gln Phe Ser Tyr Thr Phe Glu
405 410 415
Asp Val Pro Phe His Ser Ser Tyr Ala His Ser Gln Ser Leu Asp Arg
420 425 430
Leu Met Asn Pro Leu Ile Asp Gln Tyr Leu Tyr Tyr Leu Asn Lys Thr
435 440 445
Gln Thr Asn Ser Gly Thr Leu Gln Gln Ser Arg Leu Leu Phe Ser Gln
450 455 460
Ala Gly Pro Thr Asn Met Ser Leu Gln Ala Lys Asn Trp Leu Pro Gly
465 470 475 480
Pro Cys Tyr Arg Gln Gln Arg Leu Ser Lys Gln Ala Asn Asp Asn Asn
485 490 495
Asn Ser Asn Phe Pro Trp Thr Ala Ala Thr Lys Tyr His Leu Asn Gly
500 505 510
Arg Asp Ser Leu Val Asn Pro Gly Pro Ala Met Ala Ser His Lys Asp
515 520 525
Asp Glu Glu Lys Phe Phe Pro Met His Gly Thr Leu Ile Phe Gly Lys
530 535 540
Gln Gly Thr Asn Ala Asn Asp Ala Asp Leu Glu Asn Val Met Ile Thr
545 550 555 560
Asp Glu Glu Glu Ile Arg Ala Thr Asn Pro Val Ala Thr Glu Gln Tyr
565 570 575
Gly Thr Val Ser Asn Asn Leu Gln Asn Ser Asn Thr Gly Pro Thr Thr
580 585 590
Gly Thr Val Asn His Gln Gly Ala Leu Pro Gly Met Val Trp Gln Asp
595 600 605
Arg Asp Val Tyr Leu Gln Gly Pro Ile Trp Ala Lys Ile Pro His Thr
610 615 620
Asp Gly His Phe His Pro Ser Pro Leu Met Gly Gly Phe Gly Leu Lys
625 630 635 640
His Pro Pro Pro Gln Ile Met Ile Lys Asn Thr Pro Val Pro Ala Asn
645 650 655
Pro Pro Thr Asn Phe Ser Ser Ala Lys Phe Ala Ser Phe Ile Thr Gln
660 665 670
Tyr Ser Thr Gly Gln Val Ser Val Glu Ile Glu Trp Glu Leu Gln Lys
675 680 685
Glu Asn Ser Lys Arg Trp Asn Pro Glu Ile Gln Tyr Thr Ser Asn Tyr
690 695 700
Asn Lys Ser Val Asn Val Asp Phe Thr Val Asp Thr Asn Gly Val Tyr
705 710 715 720
Ser Glu Pro Arg Pro Ile Gly Thr Arg Tyr Leu Thr Arg Asn Leu
725 730 735
<210>142
<211>735
<212>PRT
<213>vp1,克隆hu.5
<400>142
Met Ala Ala Asp Gly Tyr Leu Pro Asp Trp Leu Glu Asp Thr Leu Ser
1 5 10 15
Glu Gly Ile Arg Gln Trp Trp Lys Leu Lys Pro Gly Pro Pro Pro Pro
20 25 30
Lys Pro Ala Glu Arg His Lys Asp Asp Ser Arg Gly Leu Val Leu Pro
35 40 45
Gly Tyr Lys Tyr Leu Gly Pro Phe Asn Gly Leu Asp Lys Gly Glu Pro
50 55 60
Val Asn Glu Ala Asp Ala Ala Ala Leu Glu His Asp Lys Ala Tyr Asp
65 70 75 80
Arg Gln Leu Asp Ser Gly Asp Asn Pro Tyr Leu Lys Tyr Asn His Ala
85 90 95
Asp Ala Glu Phe Gln Glu Arg Leu Lys Glu Asp Thr Ser Phe Gly Gly
100 105 110
Asn Leu Gly Arg Ala Val Phe Gln Ala Lys Lys Arg Val Leu Glu Pro
115 120 125
Leu Gly Leu Val Glu Glu Pro Val Lys Thr Ala Pro GIy Lys Lys Arg
130 135 140
Pro Val Glu His Ser Pro Val Glu Pro Asp Ser Ser Ser Gly Thr Gly
145 150 155 160
Lys Ala Gly Gln Gln Pro Ala Arg Lys Arg Leu Asn Phe Gly Gln Thr
165 170 175
Gly Asp Ala Asp Ser Val Pro Asp Pro Gln Pro Leu Gly Gln Pro Pro
180 185 190
Ala Ala Pro Ser Gly Leu Gly Ser Thr Thr Met Ala Thr Gly Ser Gly
195 200 205
Ala Pro Met Ala Asp Asn Asn Glu Gly Ala Asp Gly Val Gly Asn Ser
210 215 220
Ser Gly Asn Trp His Cys Asp Ser Gln Trp Leu Gly Asp Arg Val Ile
225 230 235 240
Thr Thr Ser Thr Arg Thr Trp Ala Leu Pro Thr Tyr Asn Asn His Leu
245 250 255
Tyr Lys Gln Ile Ser Ser Gln Ser Gly Ala Ser Asn Asp Asn His Tyr
260 265 270
Phe Gly Tyr Ser Thr Pro Trp Gly Tyr Phe Asp Phe Asn Arg Phe His
275 280 285
Cys His Phe Ser Pro Arg Asp Trp Gln Arg Leu Ile Asn Asn Asn Trp
290 295 300
Gly Phe Arg Pro Lys Arg Leu Asn Phe Lys Leu Phe Asn Ile Gln Val
305 310 315 320
Lys Glu Val Thr Gln Asn Asp Gly Thr Thr Thr Ile Ala Asn Asn Leu
325 330 335
Thr Ser Thr Val Gln Val Phe Thr Asp Ser Glu Tyr Gln Leu Pro Tyr
340 345 350
Val Leu Gly Ser Ala His Gln Gly Cys Leu Pro Pro Phe Pro Ala Asp
355 360 365
Val Phe Met Val Pro Gln Tyr Gly Tyr Leu Thr Leu Asn Asn Gly Ser
370 375 380
Gln Ala Val Gly Arg Ser Ser Phe Tyr Cys Leu Glu Tyr Phe Pro Ser
385 390 395 400
Gln Met Leu Arg Thr Gly Asn Asn Phe Gln Phe Ser Tyr Thr Phe Glu
405 410 415
Asp Val Pro Phe His Ser Ser Tyr Ala His Ser Gln Ser Leu Asp Arg
420 425 430
Leu Met Asn Pro Leu Ile Asp Gln Tyr Leu Tyr Tyr Leu Asn Lys Thr
435 440 445
Gln Thr Asn Ser Gly Thr Leu Gln Gln Ser Arg Leu Leu Phe Ser Gln
450 455 460
Ala Gly Pro Thr Asn Met Ser Leu Gln Ala Lys Asn Trp Leu Pro Gly
465 470 475 480
Pro Cys Tyr Arg Gln Gln Arg Leu Ser Lys Gln Ala Asn Asp Asn Asn
485 490 495
Asn Ser Asn Phe Pro Trp Thr Ala Ala Thr Lys Tyr His Leu Asn Gly
500 505 510
Arg Asp Ser Leu Val Asn Pro Gly Pro Ala Met Ala Ser Arg Lys Asp
515 520 525
Asp Glu Glu Lys Phe Phe Pro Met His Gly Thr Leu Ile Phe Gly Lys
530 535 540
Gln Gly Thr Asn Ala Asn Asp Ala Asp Leu Glu Asn Val Met Ile Thr
545 550 555 560
Asp Glu Glu Glu Ile Arg Ala Thr Asn Pro Val Ala Thr Glu Gln Tyr
565 570 575
Gly Thr Val Ser Asn Asn Leu Gln Asn Ser Asn Thr Gly Pro Thr Thr
580 585 590
Gly Thr Val Asn His Gln Gly Ala Leu Pro Gly Met Val Trp Gln Asp
595 600 605
Arg Asp Val Tyr Leu Gln Gly Pro Ile Trp Ala Lys Ile Pro His Thr
610 615 620
Asp Gly His Phe His Pro Ser Pro Leu Met Gly Gly Phe Gly Leu Lys
625 630 635 640
His Pro Pro Pro Gln Ile Met Ile Lys Asn Thr Pro Val Pro Ala Asn
645 650 655
Pro Pro Thr Asn Phe Ser Ser Ala Lys Phe Ala Ser Phe Ile Thr Gln
660 665 670
Tyr Ser Thr Gly Gln Val Ser Val Glu Ile Glu Trp Glu Leu Gln Lys
675 680 685
Glu Asn Ser Lys Arg Trp Asn Pro Glu Ile Gln Tyr Thr Ser Asn Tyr
690 695 700
Asn Lys Ser Val Asn Val Asp Phe Thr Val Asp Thr Asn Gly Val Tyr
705 710 715 720
Ser Glu Pro Arg Pro Ile Gly Thr Arg Tyr Leu Thr Arg Asn Leu
725 730 735
<210>143
<211>735
<212>PRT
<213>vp1,克隆hu.2
<400>143
Met Ala Ala Asp Gly Tyr Pro Pro Asp Trp Leu Glu Asp Thr Leu Ser
1 5 10 15
Glu Gly Ile Arg Gln Trp Trp Lys Leu Lys Pro Gly Pro Pro Pro Pro
20 25 30
Lys Pro Ala Glu Arg His Lys Asp Asp Ser Arg Gly Leu Val Leu Pro
35 40 45
Gly Tyr Lys Tyr Leu Gly Pro Phe Asn Gly Leu Asp Lys Gly Glu Pro
50 55 60
Val Asn Glu Ala Asp Ala Ala Ala Leu Glu His Asp Lys Ala Tyr Asp
65 70 75 80
Arg Gln Leu Asp Ser Gly Asp Asn Pro Tyr Leu Lys Tyr Asn His Ala
85 90 95
Asp Ala Glu Phe Gln Glu Arg Leu Lys Glu Asp Thr Ser Phe Gly Gly
100 105 110
Asn Leu Gly Arg Ala Val Phe Gln Ala Lys Lys Arg Val Leu Glu Pro
115 120 125
Leu Gly Leu Val Glu Glu Pro Val Lys Thr Ala Pro Gly Lys Lys Arg
130 135 140
Pro Val Glu His Ser Pro Val Glu Pro Asp Ser Ser Ser Gly Thr Gly
145 150 155 160
Lys Ala Gly Gln Arg Pro Ala Arg Lys Arg Leu Asn Phe Gly Gln Thr
165 170 175
Gly Asp Ala Asp Ser Val Pro Asp Pro Gln Pro Leu Gly Gln Pro Pro
180 185 190
Ala Ala Pro Ser Gly Leu Gly Ser Thr Thr Met Ala Thr Gly Ser Gly
195 200 205
Ala Pro Met Ala Asp Asn Asn Glu Gly Ala Asp Gly Val Gly Asn Ser
210 215 220
Ser Gly Asn Trp His Cys Asp Ser Gln Trp Leu Gly Asp Arg Val Ile
225 230 235 240
Thr Thr Ser Thr Arg Thr Trp Ala Leu Pro Thr Tyr Asn Asn His Leu
245 250 255
Tyr Lys Gln Ile Ser Ser Gln Ser Gly Ala Ser Asn Asp Asn His Tyr
260 265 270
Phe Gly Tyr Ser Thr Pro Trp Gly Tyr Phe Asp Phe Asn Arg Phe His
275 280 285
Cys His Phe Ser Pro Arg Asp Trp Gln Arg Leu Ile Asn Asn Asn Trp
290 295 300
Gly Phe Arg Pro Lys Arg Leu Asn Phe Lys Leu Phe Asn Ile Gln Val
305 310 315 320
Lys Glu Val Thr Gln Asn Asp Gly Thr Thr Thr Ile Ala Asn Asn Leu
325 330 335
Thr Ser Thr Val Gln Val Phe Thr Asp Ser Glu Tyr Gln Leu Pro Tyr
340 345 350
Val Leu Gly Ser Ala His Gln Gly Cys Leu Pro Pro Phe Pro Ala Asp
355 360 365
Val Phe Met Val Pro Gln Tyr Gly Tyr Leu Thr Leu Asn Asn Gly Ser
370 375 380
Gln Ala Val Gly Arg Ser Ser Phe Tyr Cys Leu Glu Tyr Phe Pro Ser
385 390 395 400
Gln Met Leu Arg Thr Gly Asn Asn Phe Gln Phe Ser Tyr Thr Phe Glu
405 410 415
Asp Val Pro Phe His Ser Ser Tyr Ala His Ser Gln Ser Leu Asp Arg
420 425 430
Leu Met Asn Pro Leu Ile Asp Gln Tyr Leu Tyr Tyr Leu Asn Lys Thr
435 440 445
Gln Thr Asn Ser Gly Thr Leu Gln Gln Ser Arg Leu Leu Phe Ser Gln
450 455 460
Ala Gly Pro Thr Asn Met Ser Leu Gln Ala Lys Asn Trp Leu Pro Gly
465 470 475 480
Pro Cys Tyr Arg Gln Gln Arg Leu Ser Lys Gln Ala Asn Asp Asn Asn
485 490 495
Asn Ser Asn Phe Pro Trp Thr Ala Ala Thr Lys Tyr His Leu Asn Gly
500 505 510
Arg Asp Ser Leu Val Asn Pro Gly Pro Ala Met Ala Ser His Lys Asp
515 520 525
Asp Glu Glu Lys Phe Phe Pro Met His Gly Thr Leu Ile Phe Gly Lys
530 535 540
Gln Gly Thr Asn Ala Asn Asp Ala Asp Leu Glu Asn Val Met Ile Thr
545 550 555 560
Asp Glu Glu Glu Ile Arg Ala Thr Asn Pro Val Ala Thr Glu Gln Tyr
565 570 575
Gly Thr Val Ser Asn Asn Leu Gln Asn Ser Asn Thr Gly Pro Thr Thr
580 585 590
Gly Thr Val Asn Arg Gln Gly Ala Leu Pro Gly Met Val Trp Gln Asp
595 600 605
Arg Asp Val Tyr Leu Gln Gly Pro Ile Trp Ala Lys Ile Pro His Thr
610 615 620
Asp Gly His Phe His Pro Ser Pro Leu Met Gly Gly Phe Gly Leu Lys
625 630 635 640
His Pro Pro Pro Gln Ile Met Ile Lys Asn Thr Pro Val Pro Ala Asn
645 650 655
Pro Pro Thr Asn Phe Ser Ser Ala Lys Phe Ala Ser Phe Ile Thr Gln
660 665 670
Tyr Ser Thr Gly Gln Val Ser Val Glu Ile Glu Trp Glu Leu Gln Lys
675 680 685
Glu Asn Ser Lys Arg Trp Asn Pro Glu Ile Gln Tyr Thr Ser Asn Tyr
690 695 700
Asn Lys Ser Val Asn Val Asp Phe Thr Val Asp Thr Asn Gly Val Tyr
705 710 715 720
Ser Glu Pro Arg Pro Ile Gly Thr Arg Tyr Leu Thr Arg Asn Leu
725 730 735
<210>144
<211>735
<212>PRT
<213>vp1,克隆hu.1
<400>144
Met Ala Ala Asp Gly Tyr Leu Pro Asp Trp Leu Glu Asp Thr Leu Ser
1 5 10 15
Glu Gly Ile Arg Gln Trp Trp Lys Leu Lys Pro Gly Pro Pro Pro Pro
20 25 30
Lys Pro Ala Glu Arg His Lys Asp Asp Ser Arg Gly Leu Val Leu Pro
35 40 45
Gly Tyr Lys Tyr Leu Gly Pro Phe Asn Gly Leu Asp Lys Gly Glu Pro
50 55 60
Val Asn Glu Ala Asp Ala Ala Ala Leu Glu His Asp Lys Ala Tyr Asp
65 70 75 80
Arg Gln Leu Asp Ser Gly Asp Asn Pro Tyr Leu Lys Tyr Asn His Ala
85 90 95
Asp Ala Glu Phe Gln Glu Arg Leu Lys Glu Asp Thr Ser Phe Gly Gly
100 105 110
Asn Leu Gly Arg Ala Val Phe Gln Ala Lys Lys Arg Val Leu Glu Pro
115 120 125
Leu Gly Leu Val Glu Glu Pro Val Lys Thr Ala Pro Gly Lys Lys Arg
130 135 140
Pro Val Glu His Ser Pro Val Glu Pro Asp Ser Ser Ser Gly Thr Gly
145 150 155 160
Lys Ala Gly Gln Gln Pro Ala Arg Lys Arg Leu Asn Phe Gly Gln Thr
165 170 175
Gly Asp Ala Asp Ser Val Pro Asp Pro Gln Pro Leu Gly Gln Pro Pro
180 185 190
Ala Ala Pro Ser Gly Leu Gly Ser Thr Thr Met Ala Thr Gly Ser Gly
195 200 205
Ala Pro Met Ala Asp Asn Asn Glu Gly Ala Asp Gly Val Gly Asn Ser
210 215 220
Ser Gly Asn Trp His Cys Asp Ser Gln Trp Leu Gly Asp Arg Val Ile
225 230 235 240
Thr Thr Ser Thr Arg Thr Trp Ala Leu Pro Thr Tyr Asn Asn His Leu
245 250 255
Tyr Lys Gln Ile Ser Ser Gln Ser Gly Ala Ser Asn Asp Asn His Tyr
260 265 270
Phe Gly Tyr Ser Thr Pro Trp Gly Tyr Phe Asp Phe Asn Arg Phe His
275 280 285
Cys His Phe Ser Pro Arg Asp Trp Gln Arg Leu Ile Asn Asn Asn Trp
290 295 300
Gly Phe Arg Pro Lys Arg Leu Asn Phe Lys Leu Phe Asn Ile Gln Val
305 310 315 320
Lys Glu Val Thr Gln Asn Gly Gly Thr Thr Thr Ile Ala Asn Asn Leu
325 330 335
Thr Ser Thr Val Gln Val Phe Thr Asp Ser Glu Tyr Gln Leu Pro Tyr
340 345 350
Val Leu Gly Ser Ala His Gln Gly Cys Leu Pro Pro Phe Pro Ala Asp
355 360 365
Val Phe Met Val Pro Gln Tyr Gly Tyr Leu Thr Leu Asn Asn Gly Ser
370 375 380
Gln Ala Val Gly Arg Ser Ser Phe Tyr Cys Leu Glu Tyr Phe Pro Ser
385 390 395 400
Gln Met Leu Arg Thr Gly Asn Asn Phe Gln Phe Ser Tyr Thr Phe Glu
405 410 415
Asp Val Pro Phe His Ser Ser Tyr Ala His Ser Gln Ser Leu Asp Arg
420 425 430
Leu Met Asn Pro Leu Ile Asp Gln Tyr Leu Tyr Tyr Leu Asn Lys Thr
435 440 445
Gln Thr Asn Ser Gly Thr Leu Gln Gln Ser Arg Leu Leu Phe Ser Gln
450 455 460
Ala Gly Pro Thr Asn Met Ser Leu Gln Ala Lys Asn Trp Leu Pro Gly
465 470 475 480
Pro Cys Tyr Arg Gln Gln Arg Leu Ser Lys Gln Ala Asn Gly Asn Asn
485 490 495
Asn Ser Asn Phe Pro Trp Thr Ala Ala Thr Lys Tyr His Leu Asn Gly
500 505 510
Arg Asp Ser Leu Val Asn Pro Gly Pro Ala Met Ala Ser His Lys Asp
515 520 525
Asp Glu Glu Lys Phe Phe Pro Met His Gly Thr Leu Ile Phe Gly Lys
530 535 540
Gln Gly Thr Asn Ala Asn Asp Ala Asp Leu Glu Asn Val Met Ile Thr
545 550 555 560
Asp Glu Glu Glu Ile Arg Ala Thr Asn Pro Val Ala Thr Glu Gln Tyr
565 570 575
Gly Thr Val Ser Asn Asn Leu Gln Asn Ser Asn Thr Gly Pro Thr Thr
580 585 590
Gly Thr Val Asn His Gln Gly Ala Leu Pro Gly Met Val Trp Gln Asp
595 600 605
Arg Asp Val Tyr Leu Gln Gly Pro Ile Trp Ala Lys Ile Pro His Thr
610 615 620
Asp Gly His Phe His Pro Ser Pro Leu Thr Gly Gly Phe Gly Leu Lys
625 630 635 640
His Pro Pro Pro Gln Ile Met Ile Lys Asn Thr Pro Val Pro Ala Asn
645 650 655
Pro Pro Thr Asn Phe Ser Ser Ala Lys Phe Ala Ser Phe Ile Thr Gln
660 665 670
Tyr Ser Thr Gly Gln Val Ser Val Glu Ile Glu Trp Glu Leu Gln Lys
675 680 685
Glu Asn Ser Lys Arg Trp Asn Pro Glu Ile Gln Tyr Thr Ser Asn Tyr
690 695 700
Asn Lys Ser Val Asn Val Asp Phe Thr Val Asp Thr Asn Gly Val Tyr
705 710 715 720
Ser Glu Pro Arg Pro Ile Gly Thr Arg Tyr Leu Thr Arg Asn Leu
725 730 735
<210>145
<211>736
<212>PRT
<213>vp1,克隆hu.3
<400>145
Met Ala Ala Asp Gly Tyr Leu Pro Asp Trp Leu Glu Asp Thr Leu Ser
1 5 10 15
Glu Gly Ile Arg Gln Trp Trp Lys Leu Lys Pro Gly Pro Pro Pro Pro
20 25 30
Lys Pro Ala Glu Arg His Lys Asp Asp Ser Arg Gly Leu Val Leu Pro
35 40 45
Gly Tyr Lys Tyr Leu Gly Pro Phe Asn Gly Leu Asp Lys Gly Glu Pro
50 55 60
Val Asn Glu Ala Asp Ala Ala Ala Leu Glu His Asp Lys Ala Tyr Asp
65 70 75 80
Arg Gln Leu Asp Ser Gly Asp Asn Pro Tyr Leu Lys Tyr Asn His Ala
85 90 95
Asp Ala Glu Phe Gln Glu Arg Leu Lys Glu Asp Thr Ser Phe Gly Gly
100 105 110
Asn Leu Gly Arg Ala Val Phe Gln Ala Lys Lys Arg Val Leu Glu Pro
115 120 125
Leu Arg Pro Gly Leu Arg Lys Pro Val Lys Thr Ala Pro Gly Lys Lys
130 135 140
Arg Pro Val Glu His Ser Pro Val Glu Pro Asp Ser Ser Ser Gly Thr
145 150 155 160
Gly Lys Ala Gly Gln Gln Pro Ala Arg Lys Arg Leu Asn Phe Gly Gln
165 170 175
Thr Gly Asp Ala Asp Ser Val Pro Asp Pro Gln Pro Leu Gly Gln Pro
180 185 190
Pro Ala Ala Pro Ser Gly Leu Gly Ser Thr Thr Met Ala Thr Gly Ser
195 200 205
Gly Ala Pro Met Ala Asp Asn Asn Glu Gly Ala Asp Gly Val Gly Asn
210 215 220
Ser Ser Gly Asn Trp His Cys Asp Ser Gln Trp Leu Asp Asp Arg Val
225 230 235 240
Ile Ala Thr Ser Thr Arg Thr Trp Ala Leu Pro Thr Tyr Asn Asn His
245 250 255
Leu Tyr Lys Gln Ile Ser Ser Gln Ser Gly Ala Cys Asn Asp Asn His
260 265 270
Tyr Phe Gly Tyr Ser Thr Pro Trp Gly Tyr Phe Asp Phe Asn Arg Phe
275 280 285
His Cys His Phe Ser Pro Arg Asp Trp Gln Arg Leu Ile Asn Ser Asn
290 295 300
Trp Gly Phe Arg Pro Lys Arg Leu Asn Phe Lys Leu Phe Asn Ile Gln
305 310 315 320
Val Lys Glu Val Thr Gln Asn Asp Gly Thr Thr Thr Ile Ala Asn Asn
325 330 335
Leu Thr Ser Thr Val Gln Val Phe Thr Asp Ser Glu Tyr Gln Leu Pro
340 345 350
Tyr Val Pro Gly Ser Ala His Gln Gly Cys Leu Pro Pro Phe Pro Ala
355 360 365
Asp Val Phe Met Val Pro Gln Tyr Gly Tyr Leu Thr Leu Asn Asn Gly
370 375 380
Ser Gln Ala Val Gly Arg Ser Ser Phe Tyr Cys Leu Glu Tyr Phe Pro
385 390 395 400
Ser Gln Met Leu Arg Thr Gly Asn Asn Phe Gln Phe Ser Tyr Thr Phe
405 410 415
Glu Asp Val Pro Phe His Ser Ser Tyr Ala His Cys Gln Ser Leu Asp
420 425 430
Arg Leu Met Asn Pro Leu Ile Asp Gln Tyr Leu Tyr Tyr Leu Asn Lys
435 440 445
Thr Gln Thr Asn Ser Gly Thr Leu Gln Gln Ser Arg Leu Leu Phe Ser
450 455 460
Gln Ala Gly Pro Thr Asn Met Ser Leu Gln Ala Lys Asn Trp Leu Pro
465 470 475 480
Gly Pro Cys Tyr Arg Gln Gln Arg Leu Ser Lys Gln Ala Asn Asp Asn
485 490 495
Asn Asn Cys Asn Phe Pro Trp Thr Ala Ala Thr Lys Tyr His Leu Asn
500 505 510
Gly Arg Asp Ser Leu Val Asn Pro Gly Pro Ala Met Ala Ser His Lys
515 520 525
Asp Asp Glu Glu Lys Phe Phe Pro Met His Gly Thr Leu Ile Phe Gly
530 535 540
Lys Gln Gly Thr Asn Ala Asn Asp Ala Asp Leu Glu Asn Val Met Ile
545 550 555 560
Thr Asp Glu Glu Glu Ile Arg Pro Thr Asn Pro Val Ala Thr Glu Gln
565 570 575
Tyr Gly Thr Val Ser Asn Asn Leu Gln Asn Ser Asn Thr Gly Pro Thr
580 585 590
Thr Gly Thr Val Asn His Gln Gly Ala Leu Pro Gly Met Val Trp Gln
595 600 605
Asp Arg Asp Val Tyr Leu Gln Gly Pro Ile Trp Ala Lys Ile Pro His
610 615 620
Thr Asp Gly His Phe His Pro Ser Pro Leu Met Gly Gly Phe Gly Leu
625 630 635 640
Lys His Pro Pro Pro Gln Ile Met Ile Lys Ser Thr Pro Val Pro Ala
645 650 655
Asn Pro Pro Thr Asn Phe Ser Ser Ala Lys Phe Ala Ser Ser Ile Thr
660 665 670
Gln Tyr Ser Thr Gly Gln Val Ser Val Glu Ile Glu Trp Glu Leu Gln
675 680 685
Lys Glu Asn Ser Lys Arg Trp Asn Pro Glu Ile Gln Tyr Thr Ser Asn
690 695 700
Tyr Asn Lys Ser Val Asn Val Asp Phe Thr Val Asp Thr Asn Gly Val
705 710 715 720
Tyr Ser Glu Pro Arg Pro Ile Gly Thr Arg Tyr Leu Thr Arg Asn Leu
725 730 735
<210>146
<211>735
<212>PRT
<213>vp1,克隆hu.25
<400>146
Met Ala Ala Asp Gly Tyr Leu Pro Asp Trp Leu Glu Asp Thr Leu Ser
1 5 10 15
Glu Gly Ile Arg Gln Trp Trp Lys Leu Lys Pro Gly Pro Pro Pro Pro
20 25 30
Lys Pro Ala Glu Arg His Lys Asp Gly Ser Arg Gly Leu Val Leu Pro
35 40 45
Gly Tyr Lys Tyr Leu Gly Pro Phe Asn Gly Leu Asp Lys Gly Glu Pro
50 55 60
Val Asn Glu Ala Asp Ala Ala Ala Leu Glu His Asp Lys Ala Tyr Asp
65 70 75 80
Arg Gln Leu Asn Ser Gly Asp Asn Pro Tyr Leu Lys Tyr Asn His Ala
85 90 95
Asp Ala Glu Phe Gln Glu Arg Leu Lys Glu Asp Thr Ser Phe Gly Gly
100 105 110
Asn Leu Gly Arg Ala Val Phe Gln Ala Lys Lys Arg Val Leu Glu Pro
115 120 125
Leu Gly Leu Val Glu Glu Pro Val Lys Thr Ala Pro Gly Lys Lys Arg
130 135 140
Pro Val Glu His Ser Pro Ala Glu Pro Asp Ser Ser Ser Gly Thr Gly
145 150 155 160
Lys Ala Gly Gln Gln Pro Ala Arg Lys Arg Leu Asn Phe Gly Gln Thr
165 170 175
Gly Asp Ala Asp Ser Val Pro Asp Pro Gln Pro Leu Gly Gln Pro Pro
180 185 190
Ala Ala Pro Ser Gly Leu Gly Ser Thr Thr Met Ala Thr Gly Ser Gly
195 200 205
Ala Pro Met Ala Asp Asn Asn Glu Gly Ala Asp Gly Val Gly Asn Ser
210 215 220
Ser Gly Asn Trp His Cys Asp Ser Gln Trp Leu Gly Asp Arg Val Ile
225 230 235 240
Thr Thr Ser Thr Arg Thr Trp Ala Leu Pro Thr Tyr Asn Asn His Leu
245 250 255
Tyr Lys Gln Ile Ser Ser Gln Ser Gly Ala Ser Asn Asp Asn His Tyr
260 265 270
Phe Gly Tyr Ser Thr Pro Trp Gly Tyr Phe Asp Phe Asn Arg Phe His
275 280 285
Cys His Phe Ser Pro Arg Asp Trp Gln Arg Leu Ile Asn Asn Asn Trp
290 295 300
Gly Phe Arg Pro Lys Arg Leu Asn Phe Lys Leu Phe Asn Ile Gln Val
305 310 315 320
Lys Glu Val Thr Gln Asn Asp Gly Thr Thr Thr Ile Ala Asn Asn Leu
325 330 335
Thr Ser Thr Val Gln Val Phe Thr Asp Ser Glu Tyr Gln Leu Pro Tyr
340 345 350
Val Leu Gly Ser Ala His Gln Gly Cys Leu Pro Pro Phe Pro Ala Asp
355 360 365
Val Phe Met Val Pro Gln Tyr Gly Tyr Leu Thr Leu Asn Asn Gly Ser
370 375 380
Gln Ala Val Gly Arg Ser Pro Phe Tyr Cys Leu Glu Tyr Phe Pro Ser
385 390 395 400
Gln Met Leu Arg Thr Gly Asn Asn Phe Gln Phe Ser Tyr Thr Phe Glu
405 410 415
Asp Val Pro Phe His Ser Ser Tyr Ala His Ser Gln Ser Leu Asp Arg
420 425 430
Leu Met Asn Pro Leu Ile Asp Gln Tyr Leu Tyr Tyr Leu Asn Lys Thr
435 440 445
Gln Thr Asn Ser Gly Thr Leu Gln Gln Ser Arg Leu Leu Phe Ser Gln
450 455 460
Ala Gly Pro Thr Asn Met Ser Leu Gln Ala Lys Asn Trp Leu Pro Gly
465 470 475 480
Pro Cys Tyr Arg Gln Gln Arg Leu Ser Lys Gln Ala Asn Asp Asn Asn
485 490 495
Asn Ser Asn Phe Pro Trp Thr Ala Ala Thr Lys Tyr His Leu Asn Gly
500 505 510
Arg Asp Ser Leu Val Asn Pro Gly Pro Ala Met Ala Ser His Lys Asp
515 520 525
Asp Glu Glu Lys Phe Phe Pro Met His Gly Thr Leu Ile Phe Gly Lys
530 535 540
Gln Gly Thr Asn Ala Asn Asp Ala Asp Leu Glu Asn Val Met Ile Thr
545 550 555 560
Asp Glu Glu Glu Ile Arg Thr Thr Asn Pro Val Ala Thr Glu Gln Tyr
565 570 575
Gly Thr Val Ser Asn Asn Leu Gln Asn Ser Asn Thr Gly Pro Thr Thr
580 585 590
Gly Thr Val Asn His Gln Gly Ala Leu Pro Gly Met Val Trp Gln Asp
595 600 605
Arg Asp Val Tyr Leu Gln Gly Pro Ile Trp Ala Lys Ile Pro His Thr
610 615 620
Asp Gly His Phe His Pro Ser Pro Leu Met Gly Gly Phe Gly Leu Lys
625 630 635 640
His Pro Pro Pro Gln Ile Met Ile Lys Asn Thr Pro Val Pro Ala Asn
645 650 655
Pro Pro Thr Asn Phe Ser Ser Ala Lys Phe Ala Ser Phe Ile Thr Gln
660 665 670
Tyr Ser Thr Gly Gln Val Ser Val Glu Ile Glu Trp Glu Leu Gln Lys
675 680 685
Glu Asn Ser Lys Arg Trp Asn Pro Glu Ile Gln Tyr Thr Ser Asn Tyr
690 695 700
Asn Lys Ser Val Asn Val Asp Phe Thr Val Asp Asn Asn Gly Val Tyr
705 710 715 720
Ser Glu Pro Arg Pro Ile Gly Thr Arg Tyr Leu Thr Arg Asn Leu
725 730 735
<210>147
<211>735
<212>PRT
<213>vp1,克隆hu.15
<400>147
Met Ala Ala Asp Gly Tyr Leu Pro Asp Trp Leu Glu Asp Thr Leu Ser
1 5 10 15
Glu Gly Ile Arg Gln Trp Trp Lys Leu Lys Pro Gly Pro Pro Pro Pro
20 25 30
Lys Pro Ala Glu Arg His Lys Asp Asp Ser Arg Gly Leu Val Leu Pro
35 40 45
Gly Tyr Lys Tyr Leu Gly Pro Phe Asn Gly Leu Tyr Lys Gly Glu Pro
50 55 60
Val Asp Glu Ala Asp Ala Ala Ala Leu Glu His Asp Lys Ala Tyr Asp
65 70 75 80
Arg Gln Leu Asp Ser Gly Asp Asn Pro Tyr Leu Lys Tyr Asn His Ala
85 90 95
Asp Ala Glu Phe Gln Glu Arg Leu Lys Glu Asp Thr Ser Phe Gly Gly
100 105 110
Asn Leu Gly Arg Ala Val Phe Gln Ala Lys Lys Arg Val Leu Glu Pro
115 120 125
Leu Gly Leu Val Gly Glu Pro Val Lys Thr Ala Pro Gly Lys Lys Arg
130 135 140
Pro Val Glu His Ser Pro Val Glu Pro Asp Ser Ser Ser Gly Thr Gly
145 150 155 160
Lys Ala Gly Asn Gln Pro Ala Arg Lys Arg Leu Asn Phe Gly Gln Thr
165 170 175
Gly Asp Ala Asp Ser Val Pro Asp Pro Gln Pro Leu Gly Gln Pro Pro
180 185 190
Ala Ala Pro Ser Gly Leu Gly Ser Thr Thr Met Ala Thr Gly Ser Gly
195 200 205
Ala Pro Val Ala Asp Asn Asn Glu Gly Ala Asp Gly Val Gly Asn Ser
210 215 220
Ser Gly Asn Trp His Cys Asp Ser Gln Trp Leu Gly Asp Arg Val Ile
225 230 235 240
Thr Thr Ser Thr Arg Thr Trp Ala Leu Pro Thr Tyr Asn Asn His Leu
245 250 255
Tyr Lys Gln Ile Ser Ser Gln Ser Gly Ala Ser Asn Asp Asn His Tyr
260 265 270
Phe Gly Tyr Ser Thr Pro Trp Gly Tyr Phe Asp Phe Asn Arg Phe His
275 280 285
Cys His Phe Ser Pro Arg Asp Arg Gln Arg Leu Ile Asn Asn Asn Trp
290 295 300
Gly Phe Arg Pro Lys Arg Leu Asn Phe Lys Leu Phe Asn Ile Gln Val
305 310 315 320
Lys Glu Val Thr Gln Asn Asp Gly Thr Thr Thr Ile Ala Asn Asn Leu
325 330 335
Thr Ser Thr Val Gln Val Phe Thr Asp Ser Gly Tyr Gln Leu Pro Tyr
340 345 350
Val Leu Gly Leu Ala His Gln Gly Cys Leu Pro Pro Phe Pro Ala Asp
355 360 365
Val Phe Met Val Pro Gln Tyr Gly Tyr Leu Thr Leu Asn Asn Gly Ser
370 375 380
Gln Ala Val Gly Arg Ser Ser Phe Tyr Cys Leu Glu Tyr Phe Pro Ser
385 390 395 400
Gln Met Leu Arg Thr Gly Asn Asn Phe Gln Phe Ser Tyr Thr Phe Glu
405 410 415
Asp Val Pro Phe His Ser Ser Tyr Ala His Ser Gln Ser Leu Asp Arg
420 425 430
Leu Met Asn Pro Leu Ile Asp Gln Tyr Leu Tyr Tyr Leu Asn Lys Thr
435 440 445
Gln Ser Asn Ser Gly Thr Leu Gln Gln Ser Arg Leu Leu Phe Ser Gln
450 455 460
Ala Gly Pro Thr Ser Met Ser Leu Gln Ala Lys Asn Trp Leu Pro Gly
465 470 475 480
Pro Cys Tyr Arg Gln Gln Arg Leu Ser Lys Gln Ala Asn Asp Asn Asn
485 490 495
Asn Ser Asn Phe Pro Trp Thr Ala Ala Thr Lys Tyr His Leu Asn Gly
500 505 510
Arg Asp Ser Leu Val Asn Pro Gly Pro Ala Met Ala Ser His Lys Asp
515 520 525
Asp Glu Glu Lys Phe Phe Pro Met His Gly Thr Leu Ile Phe Gly Lys
530 535 540
Gln Gly Thr Asn Ala Asn Asp Ala Asp Leu Asp Asn Val Met Ile Thr
545 550 555 560
Asp Glu Glu Glu Ile Arg Thr Thr Asn Pro Val Ala Thr Glu Gln Tyr
565 570 575
Gly Tyr Val Ser Asn Asn Leu Gln Asn Ser Asn Thr Gly Pro Thr Thr
580 585 590
Gly Thr Val Asn His Gln Gly Ala Leu Pro Gly Met Val Trp Gln Asp
595 600 605
Arg Asp Val Tyr Leu Gln Gly Pro Ile Trp Ala Lys Ile Pro His Thr
610 615 620
Asp Gly His Phe His Pro Ser Pro Leu Met Gly Gly Phe Gly Leu Lys
625 630 635 640
His Pro Pro Pro Gln Ile Met Ile Lys Asn Thr Pro Val Pro Ala Asn
645 650 655
Pro Pro Thr Asn Phe Ser Ser Ala Lys Phe Ala Ser Phe Ile Thr Gln
660 665 670
Tyr Ser Thr Gly Gln Val Ser Val Glu Ile Glu Trp Glu Leu Gln Lys
675 680 685
Glu Asp Ser Lys Arg Trp Asn Pro Glu Ile Gln Tyr Thr Ser Asn Tyr
690 695 700
Asn Lys Pro Val Asn Val Asp Phe Thr Val Asp Thr Asn Gly Val Tyr
705 710 715 720
Ser Glu Pro Arg Pro Ile Gly Thr Arg Tyr Leu Thr Arg Asn Leu
725 730 735
<210>148
<211>735
<212>PRT
<213>vp1,克隆hu.16
<400>148
Met Ala Ala Asp Gly Tyr Leu Pro Asp Trp Leu Glu Asp Thr Leu Ser
1 5 10 15
Glu Gly Ile Arg Gln Trp Trp Lys Leu Lys Pro Gly Pro Pro Pro Pro
20 25 30
Lys Pro Ala Glu Arg His Lys Asp Asp Ser Arg Gly Leu Val Leu Pro
35 40 45
Gly Tyr Lys Tyr Leu Gly Pro Phe Asn Gly Leu Tyr Lys Gly Glu Pro
50 55 60
Val Asn Glu Ala Asp Ala Ala Ala Leu Glu His Asp Lys Ala Tyr Asp
65 70 75 80
Arg Gln Leu Asp Ser Gly Asp Asn Pro Tyr Leu Lys Tyr Asn His Ala
85 90 95
Gly Ala Glu Phe Gln Glu Arg Leu Lys Glu Asp Thr Ser Phe Gly Gly
100 105 110
Asn Leu Gly Arg Ala Val Phe Gln Ala Lys Lys Arg Val Leu Glu Pro
115 120 125
Leu Gly Leu Val Glu Glu Pro Val Lys Thr Ala Pro Gly Lys Lys Arg
130 135 140
Pro Val Glu His Ser Pro Val Glu Pro Asp Ser Ser Ser Gly Thr Gly
145 150 155 160
Lys Ala Gly Asn Gln Pro Ala Arg Lys Arg Leu Asn Phe Gly Gln Thr
165 170 175
Gly Asp Ala Asp Ser Val Pro Asp Pro Gln Pro Leu Gly Gln Pro Pro
180 185 190
Ala Ala Pro Ser Gly Leu Gly Ser Thr Thr Met Ala Thr Gly Ser Gly
195 200 205
Ala Pro Val Ala Asp Asn Asn Glu Gly Ala Asp Gly Val Gly Asn Ser
210 215 220
Ser Gly Asn Trp His Cys Asp Ser Gln Trp Leu Gly Asp Arg Val Ile
225 230 235 240
Thr Thr Ser Thr Arg Thr Trp Ala Leu Pro Thr Tyr Asn Asn His Leu
245 250 255
Tyr Lys Gln Ile Ser Ser Gln Ser Gly Ala Ser Asn Asp Asn His Tyr
260 265 270
Phe Gly Tyr Ser Thr Pro Trp Gly Tyr Phe Asp Phe Asn Arg Phe His
275 280 285
Cys His Phe Ser Pro Arg Asp Trp Gln Arg Leu Ile Asn Asn Asn Trp
290 295 300
Gly Phe Arg Pro Lys Arg Leu Asn Phe Lys Leu Phe Asn Ile Gln Val
305 310 315 320
Lys Glu Val Thr Gln Asn Asp Gly Thr Thr Thr Ile Ala Asn Asn Leu
325 330 335
Thr Ser Thr Val Gln Val Phe Thr Asp Ser Glu Tyr Gln Leu Pro Tyr
340 345 350
Val Leu Gly Ser Ala His Gln Gly Cys Leu Pro Pro Phe Pro Ala Asp
355 360 365
Val Phe Met Val Pro Gln Tyr Gly Tyr Leu Thr Leu Asn Asn Gly Ser
370 375 380
Gln Ala Val Gly Arg Ser Ser Phe Tyr Cys Leu Glu Tyr Phe Pro Ser
385 390 395 400
Gln Met Leu Arg Thr Gly Asn Asn Phe Gln Phe Ser Tyr Thr Phe Glu
405 410 415
Asp Val Pro Phe His Ser Ser Tyr Ala His Ser Gln Ser Leu Asp Arg
420 425 430
Leu Met Asn Pro Leu Ile Asp Gln Tyr Leu Tyr Tyr Leu Asn Lys Thr
435 440 445
Gln Ser Asn Ser Gly Thr Leu Gln Gln Ser Arg Leu Leu Phe Ser Gln
450 455 460
Ala Gly Pro Thr Ser Met Ser Leu Gln Ala Lys Asn Trp Leu Pro Gly
465 470 475 480
Pro Cys Tyr Arg Gln Gln Arg Leu Ser Lys Gln Ala Asn Asp Asn Asn
485 490 495
Asn Ser Asn Phe Pro Trp Thr Ala Ala Thr Lys Tyr His Leu Asn Gly
500 505 510
Arg Asp Ser Leu Val Asn Pro Gly Pro Ala Met Ala Ser His Lys Asp
515 520 525
Asp Glu Glu Lys Phe Phe Pro Met His Gly Thr Leu Ile Phe Gly Lys
530 535 540
Gln Gly Thr Asn Ala Asn Asp Ala Asp Leu Asp Asn Val Met Ile Thr
545 550 555 560
Asp Glu Glu Glu Ile Arg Thr Thr Asn Pro Val Ala Thr Glu Gln Tyr
565 570 575
Gly Tyr Val Ser Asn Asn Leu Gln Asp Ser Asn Thr Gly Pro Thr Thr
580 585 590
Gly Thr Val Asn His Gln Gly Ala Leu Pro Gly Met Val Trp Gln Asp
595 600 605
Arg Asp Val Tyr Leu Gln Gly Pro Ile Trp Ala Lys Ile Pro His Thr
610 615 620
Asp Gly His Phe His Pro Ser Pro Leu Met Gly Gly Phe Gly Leu Lys
625 630 635 640
His Pro Pro Pro Gln Ile Met Ile Lys Asn Thr Pro Val Pro Ala Asn
645 650 655
Pro Pro Thr Asn Phe Ser Ser Ala Lys Phe Ala Ser Phe Ile Thr Gln
660 665 670
Tyr Ser Thr Gly Gln Val Ser Val Glu Ile Glu Trp Glu Leu Gln Lys
675 680 685
Glu Asn Ser Lys Arg Trp Asn Pro Glu Ile Gln Tyr Thr Ser Asn Tyr
690 695 700
Asn Lys Ser Val Asn Val Asp Phe Thr Val Asp Thr Asn Gly Val Tyr
705 710 715 720
Ser Glu Pro Arg Pro Ile Gly Thr Arg Tyr Leu Thr Arg Asn Leu
725 730 735
<210>149
<211>735
<212>PRT
<213>vp1,克隆hu.18
<400>149
Met Ala Ala Asp Gly Tyr Leu Pro Asp Trp Leu Glu Asp Thr Leu Ser
1 5 10 15
Glu Gly Ile Arg Gln Trp Trp Lys Leu Lys Pro Gly Pro Pro Pro Pro
20 25 30
Lys Pro Ala Glu Arg His Lys Asp Asp Ser Arg Gly Leu Val Leu Pro
35 40 45
Gly Tyr Lys Tyr Leu Gly Pro Phe Asn Gly Leu Asp Lys Gly Glu Pro
50 55 60
Val Asn Glu Ala Asp Ala Ala Ala Leu Glu His Asp Lys Ala Tyr Asp
65 70 75 80
Arg Gln Leu Glu Ser Gly Asp Asn Pro Tyr Leu Lys Tyr Asn His Ala
85 90 95
Asp Ala Glu Phe Gln Glu Arg Leu Lys Glu Asp Thr Ser Phe Gly Gly
100 105 110
Asn Leu Gly Arg Ala Val Phe Gln Ala Lys Lys Arg Val Leu Glu Pro
115 120 125
Leu Gly Leu Val Glu Glu Pro Val Lys Thr Ala Pro Gly Lys Lys Arg
130 135 140
Pro Val Glu His Ser Pro Val Glu Pro Asp Ser Ser Ser Gly Thr Gly
145 150 155 160
Lys Ala Gly Gln Gln Pro Ala Arg Lys Arg Leu Asn Phe Gly Gln Thr
165 170 175
Gly Asp Ala Asp Ser Val Pro Asp Pro Gln Pro Leu Gly Gln Pro Pro
180 185 190
Ala Ala Pro Ser Gly Leu Gly Ser Thr Thr Met Ala Ser Gly Ser Gly
195 200 205
Ala Pro Val Ala Asp Asn Asn Glu Gly Ala Asp Gly Val Gly Asn Ser
210 215 220
Ser Gly Asn Trp His Cys Asp Ser Gln Trp Leu Gly Asp Arg Val Ile
225 230 235 240
Thr Thr Ser Thr Arg Thr Trp Ala Leu Pro Thr Tyr Asn Asn His Leu
245 250 255
Tyr Lys Gln Ile Ser Ser Gln Ser Gly Ala Ser Asn Asp Asn His Tyr
260 265 270
Phe Gly Tyr Ser Thr Pro Trp Gly Tyr Phe Asp Phe Asn Arg Phe His
275 280 285
Cys His Phe Ser Pro Arg Asp Trp Gln Arg Leu Ile Asn Asn Ser Trp
290 295 300
Gly Phe Arg Pro Lys Arg Leu Asn Phe Lys Leu Phe Asn Ile Gln Val
305 310 315 320
Lys Glu Val Thr Gln Asn Asp Gly Thr Thr Thr Ile Ala Asn Asn Leu
325 330 335
Thr Ser Thr Val Gln Val Phe Thr Asp Ser Glu Tyr Gln Leu Pro Tyr
340 345 350
Val Leu Gly Ser Ala His Gln Gly Cys Leu Pro Pro Phe Pro Ala Asp
355 360 365
Val Phe Met Val Pro Gln Tyr Gly Tyr Leu Thr Leu Asn Asn Gly Ser
370 375 380
Gln Ala Val Gly Arg Ser Ser Phe Tyr Cys Leu Glu Tyr Phe Pro Ser
385 390 395 400
Gln Met Leu Arg Thr Gly Asn Asn Phe Gln Phe Ser Tyr Thr Phe Glu
405 410 415
Asp Val Pro Phe His Ser Ser Tyr Ala His Ser Gln Ser Leu Asp Arg
420 425 430
Leu Leu Asn Pro Leu Ile Asp Gln Tyr Leu Tyr Tyr Leu Asn Lys Thr
435 440 445
Gln Ser Asn Ser Gly Thr Leu Gln Gln Ser Arg Leu Leu Phe Ser Gln
450 455 460
Ala Gly Pro Thr Ser Met Ser Leu Gln Ala Lys Asn Trp Leu Pro Gly
465 470 475 480
Pro Cys Tyr Arg Gln Gln Arg Leu Ser Lys Gln Ala Asn Asp Asn Asn
485 490 495
Asn Ser Asn Phe Pro Trp Thr Ala Ala Thr Lys Tyr His Leu Asn Gly
500 505 510
Arg Asp Ser Leu Val Asn Pro Gly Pro Ala Met Ala Ser His Lys Asp
515 520 525
Asp Glu Glu Lys Phe Phe Pro Met His Gly Thr Leu Ile Phe Gly Lys
530 535 540
Gln Gly Thr Asn Ala Asn Asp Ala Asp Leu Asp Asn Val Met Ile Thr
545 550 555 560
Asp Glu Glu Glu Ile Arg Thr Thr Asn Pro Val Ala Thr Glu Gln Tyr
565 570 575
Gly Tyr Val Ser Asn Asn Leu Gln Asn Ser Asn Thr Gly Pro Thr Thr
580 585 590
Gly Thr Val Asn His Gln Gly Ala Leu Pro Gly Met Val Trp Gln Asp
595 600 605
Arg Asp Val Tyr Leu Gln Gly Pro Ile Trp Ala Lys Ile Pro His Thr
610 615 620
Asp Gly His Phe His Pro Ser Pro Leu Met Gly Gly Phe Gly Leu Lys
625 630 635 640
His Pro Pro Pro Gln Ile Met Ile Lys Asn Thr Pro Val Pro Ala Asn
645 650 655
Pro Pro Thr Asn Phe Ser Ser Ser Lys Phe Ala Ser Phe Ile Thr Gln
660 665 670
Tyr Ser Thr Gly Gln Val Ser Val Glu Ile Glu Trp Glu Leu Gln Lys
675 680 685
Glu Asn Ser Lys Arg Trp Asn Pro Glu Ile Gln Tyr Thr Ser Asn Tyr
690 695 700
Asn Lys Ser Val Asn Val Asp Phe Thr Val Asp Thr Asn Gly Val Tyr
705 710 715 720
Ser Glu Pro Arg Pro Ile Gly Thr Arg Tyr Pro Thr Arg Asn Leu
725 730 735
<210>150
<211>735
<212>PRT
<213>vp1,克隆hu.7
<400>150
Met Ala Ala Asp Gly Tyr Leu Pro Asp Trp Leu Glu Asp Thr Leu Ser
1 5 10 15
Glu Gly Ile Arg Gln Trp Trp Lys Leu Lys Pro Gly Pro Pro Pro Pro
20 25 30
Lys Pro Ala Glu Arg His Lys Asp Asp Ser Arg Gly Leu Val Leu Pro
35 40 45
Gly Tyr Lys Tyr Leu Gly Pro Phe Asn Gly Leu Asp Lys Gly Glu Pro
50 55 60
Val Asn Glu Ala Asp Ala Ala Ala Leu Glu His Asp Lys Ala Tyr Asp
65 70 75 80
Arg Gln Leu Asp Ser Gly Asp Asn Pro Tyr Leu Lys Tyr Asn His Ala
85 90 95
Asp Ala Glu Phe Gln Glu Arg Leu Lys Glu Asp Thr Ser Phe Gly Gly
100 105 110
Asn Leu Gly Arg Ala Val Phe Gln Ala Lys Lys Arg Val Leu Glu Pro
115 120 125
Leu Gly Leu Val Glu Gly Pro Val Lys Thr Ala Pro Gly Lys Lys Arg
130 135 140
Pro Val Glu His Ser Pro Ala Glu Pro Asp Ser Ser Ser Gly Thr Gly
145 150 155 160
Lys Ala Gly Gln Gln Pro Ala Arg Lys Arg Leu Asn Phe Gly Gln Thr
165 170 175
Gly Asp Ala Asp Ser Val Pro Asp Pro Gln Pro Leu Gly Gln Pro Pro
180 185 190
Ala Ala Pro Ser Gly Leu Gly Ser Thr Thr Met Ala Thr GIy Ser Gly
195 200 205
Ala Pro Met Ala Asp Asn Asn Glu Gly Ala Asp Gly Val Gly Asn Ser
210 215 220
Ser Gly Asn Trp His Cys Asp Ser Gln Trp Leu Gly Asp Arg Val Ile
225 230 235 240
Thr Thr Ser Thr Arg Thr Trp Ala Leu Pro Thr Tyr Asn Asn His Leu
245 250 255
Tyr Lys Gln Ile Ser Ser Gln Ser Gly Ala Ser Asn Asp Asn His Tyr
260 265 270
Phe Gly Tyr Ser Thr Pro Trp Gly Tyr Phe Asp Phe Asn Arg Phe His
275 280 285
Cys His Phe Ser Pro Arg Asp Trp Gln Arg Leu Ile Asn Asn Asn Trp
290 295 300
Gly Phe Arg Pro Lys Arg Leu Asn Phe Lys Leu Phe Asn Ile Gln Val
305 310 315 320
Lys Glu Val Thr Gln Asn Asp Gly Thr Thr Thr Ile Ala Asn Asn Leu
325 330 335
Thr Ser Thr Val Gln Val Phe Thr Asp Ser Glu Tyr Gln Leu Pro Tyr
340 345 350
Val Leu Gly Ser Ala His Gln Gly Cys Leu Pro Pro Phe Pro Ala Asp
355 360 365
Val Phe Met Val Pro Gln Tyr Gly Tyr Leu Thr Leu Asn Asn Gly Ser
370 375 380
Gln Ala Val Gly Arg Ser Ser Phe Tyr Cys Leu Glu Tyr Phe Pro Ser
385 390 395 400
Gln Met Leu Arg Thr Gly Asn Asn Phe Gln Phe Ser Tyr Thr Phe Glu
405 410 415
Asp Val Pro Phe His Ser Ser Tyr Ala His Ser Gln Ser Leu Asp Arg
420 425 430
Leu Met Asn Pro Leu Ile Asp Gln Tyr Leu Tyr Tyr Leu Asn Lys Thr
435 440 445
Gln Ser Asn Ser Gly Thr Leu Gln Gln Ser Arg Leu Leu Phe Ser Gln
450 455 460
Ala Gly Pro Thr Ser Met Ser Leu Gln Ala Lys Asn Trp Leu Pro Gly
465 470 475 480
Pro Cys Tyr Arg Gln Gln Arg Leu Ser Lys Gln Ala Asn Asp Asn Asn
485 490 495
Asn Ser Asn Phe Pro Trp Thr Ala Ala Thr Lys Tyr His Leu Asn Gly
500 505 510
Arg Asp Ser Leu Val Asn Pro Gly Pro Ala Met Ala Ser His Lys Asp
515 520 525
Asp Glu Glu Lys Phe Phe Pro Met His Gly Thr Leu Ile Phe Gly Lys
530 535 540
Gln Gly Thr Asn Ala Asn Asp Ala Asp Leu Asp Asn Val Met Ile Thr
545 550 555 560
Asp Glu Glu Glu Ile Arg Thr Thr Asn Pro Val Ala Thr Glu Gln Tyr
565 570 575
Gly Tyr Val Ser Asn Asn Leu Gln Asn Ser Asn Thr Gly Pro Thr Thr
580 585 590
Gly Thr Val Asn His Gln Gly Ala Leu Pro Gly Met Val Trp Gln Asp
595 600 605
Arg Asp Val Tyr Leu Gln Gly Pro Ile Trp Ala Lys Ile Pro His Thr
610 615 620
Asp Gly His Phe His Pro Ser Pro Leu Met Gly Gly Phe Gly Leu Lys
625 630 635 640
His Pro Pro Pro Gln Ile Met Ile Lys Asn Thr Pro Val Pro Ala Asn
645 650 655
Pro Pro Thr Asn Phe Ser Ser Ala Lys Phe Ala Ser Phe Ile Thr Gln
660 665 670
Tyr Ser Thr Gly Gln Val Ser Val Glu Ile Glu Trp Glu Leu Gln Lys
675 680 685
Glu Asn Ser Lys Arg Trp Asn Pro Glu Ile Gln Tyr Thr Ser Asn Tyr
690 695 700
Asn Lys Ser Val Asn Val Asp Phe Thr Val Asp Thr Asn Gly Val Tyr
705 710 715 720
Ser Glu Pro Arg Pro Ile Gly Thr Arg Tyr Leu Thr Arg Asn Leu
725 730 735
<210>151
<211>735
<212>PRT
<213>vp1,克隆hu.8
<400>151
Met Ala Ala Asp Gly Tyr Leu Pro Asp Trp Leu Glu Asp Thr Leu Ser
1 5 10 15
Glu Gly Thr Arg Gln Trp Trp Lys Leu Lys Pro Gly Pro Pro Pro Pro
20 25 30
Lys Pro Ala Glu Arg His Lys Asp Asp Ser Arg Gly Leu Val Leu Pro
35 40 45
Gly Tyr Lys Tyr Leu Gly Pro Phe Asn Gly Leu Asp Lys Gly Glu Pro
50 55 60
Val Asn Glu Ala Asp Ala Ala Ala Leu Glu His Asp Lys Ala Tyr Asp
65 70 75 80
Arg Gln Leu Asp Ser Gly Asp Asn Pro Tyr Leu Lys Tyr Asn His Ala
85 90 95
Asp Ala Glu Phe Gln Glu Arg Leu Lys Glu Asp Thr Ser Phe Gly Gly
100 105 110
Asn Leu Gly Arg Ala Val Phe Gln Ala Lys Lys Arg Val Leu Glu Pro
115 120 125
Leu Gly Leu Val Glu Glu Pro Val Lys Thr Ala Pro Gly Lys Lys Arg
130 135 140
Pro Val Glu His Ser Pro Ala Glu Pro Asp Ser Ser Ser Gly Thr Gly
145 150 155 160
Lys Ala Gly Gln Gln Pro Ala Arg Lys Arg Leu Asn Phe Gly Gln Thr
165 170 175
Gly Asp Ala Asp Ser Val Pro Asp Pro Gln Pro Leu Gly Gln Pro Pro
180 185 190
Ala Ala Pro Ser Gly Leu Gly Ser Thr Thr Met Ala Thr Gly Ser Gly
195 200 205
Ala Pro Met Ala Asp Asn Asn Glu Gly Ala Asp Gly Val Gly Asn Ser
210 215 220
Ser Gly Asn Trp His Cys Asp Ser Gln Trp Leu Gly Asp Arg Val Ile
225 230 235 240
Thr Thr Ser Thr Arg Thr Trp Ala Leu Pro Thr Tyr Asn Asn His Leu
245 250 255
Tyr Lys Gln Ile Ser Ser Gln Ser Gly Ala Ser Asn Asp Asn His Tyr
260 265 270
Phe Gly Tyr Ser Thr Pro Trp Gly Tyr Phe Asp Phe Asn Arg Phe His
275 280 285
Cys His Phe Ser Pro Arg Asp Trp Gln Arg Leu Ile Asn Asn Asn Trp
290 295 300
Gly Phe Arg Pro Lys Arg Leu Asn Phe Lys Leu Phe Asn Ile Gln Val
305 310 315 320
Lys Glu Val Thr Gln Asn Asp Gly Thr Thr Thr Ile Ala Asn Asn Leu
325 330 335
Thr Ser Thr Val Gln Val Phe Thr Asp Ser Glu Tyr Gln Leu Pro Tyr
340 345 350
Val Leu Gly Ser Ala His Gln Gly Cys Leu Pro Pro Phe Pro Ala Asp
355 360 365
Val Phe Met Val Pro Gln Tyr Gly Tyr Leu Thr Leu Asn Asn Gly Ser
370 375 380
Gln Ala Val Gly Arg Ser Ser Phe Tyr Cys Leu Glu Tyr Phe Pro Ser
385 390 395 400
Gln Met Leu Arg Thr Gly Asn Asn Phe Gln Phe Ser Tyr Thr Phe Glu
405 410 415
Asp Val Pro Phe His Ser Ser Tyr Ala His Ser Gln Ser Leu Asp Arg
420 425 430
Leu Met Asn Pro Leu Ile Asp Gln Tyr Leu Tyr Tyr Leu Asn Lys Thr
435 440 445
Gln Ser Asn Ser Gly Thr Leu Gln Gln Ser Arg Leu Leu Phe Ser Gln
450 455 460
Ala Gly Pro Thr Ser Met Ser Leu Gln Ala Lys Asn Trp Leu Pro Gly
465 470 475 480
Pro Cys Tyr Arg Gln Gln Arg Leu Ser Lys Gln Ala Asn Asp Asn Asn
485 490 495
Asn Ser Asn Phe Pro Trp Thr Ala Ala Thr Lys Tyr His Leu Asn Gly
500 505 510
Arg Asp Ser Leu Val Asn Pro Gly Pro Ala Met Ala Ser His Lys Asp
515 520 525
Asp Glu Glu Lys Phe Phe Pro Met His Gly Thr Leu Ile Phe Gly Lys
530 535 540
Gln Gly Thr Asn Ala Asn Asp Ala Asp Leu Asp Asn Val Met Ile Thr
545 550 555 560
Asp Glu Glu Glu Ile Arg Thr Thr Asn Pro Val Ala Thr Glu Gln Tyr
565 570 575
Gly Tyr Val Ser Asn Asn Leu Gln Asn Ser Asn Thr Gly Pro Thr Thr
580 585 590
Gly Thr Val Asn His Gln Gly Ala Leu Pro Gly Met Val Trp Gln Asp
595 600 605
Arg Asp Val Tyr Leu Gln Gly Pro Ile Trp Ala Lys Ile Pro His Thr
610 615 620
Asp Gly His Phe His Pro Ser Pro Leu Met Gly Gly Phe Gly Leu Lys
625 630 635 640
His Pro Pro Pro Gln Ile Met Ile Lys Asn Thr Pro Val Pro Ala Asn
645 650 655
Pro Pro Thr Asn Phe Ser Ser Ala Lys Phe Ala Ser Phe Ile Thr Gln
660 665 670
Tyr Ser Thr Gly Gln Val Ser Val Glu Ile Glu Trp Glu Leu Gln Lys
675 680 685
Glu Asn Ser Lys Arg Trp Asn Pro Glu Ile Gln Tyr Thr Ser Asn Tyr
690 695 700
Asn Lys Ser Val Asn Val Asp Phe Thr Val Asp Thr Asn Gly Val Tyr
705 710 715 720
Ser Glu Pro Arg Pro Ile Gly Thr Arg Tyr Leu Thr Arg Asn Leu
725 730 735
<210>152
<211>735
<212>PRT
<213>vpl,克隆rh.56
<400>152
Met Ala Ala Asp Gly Tyr Leu Pro Asp Trp Leu Glu Asp Thr Leu Ser
1 5 10 15
Glu Gly Ile Arg Gln Trp Trp Lys Leu Lys Pro Gly Pro Pro Pro Pro
20 25 30
Lys Pro Ala Glu Arg His Lys Asp Asp Ser Arg Gly Leu Val Leu Pro
35 40 45
Gly Tyr Lys Tyr Leu Gly Pro Phe Asn Gly Leu Asp Lys Gly Glu Pro
50 55 60
Val Asn Glu Ala Asp Ala Ala Ala Leu Glu His Asp Lys Ala Tyr Asp
65 70 75 80
Arg Gln Leu Asp Ser Gly Asp Asn Pro Tyr Leu Lys Tyr Asn His Ala
85 90 95
Asp Ala Glu Phe Gln Glu Arg Leu Lys Glu Asp Thr Ser Phe Gly Gly
100 105 110
Asn Leu Gly Arg Ala Val Phe Gln Ala Lys Lys Arg Val Leu Glu Pro
115 120 125
Leu Gly Leu Val Glu Glu Pro Val Lys Thr Ala Pro Gly Lys Lys Arg
130 135 140
Pro Val Glu His Ser Pro Ala Glu Pro Asp Ser Ser Ser Gly Thr Gly
145 150 155 160
Lys Ala Gly Gln Gln Pro Ala Arg Lys Arg Leu Asn Phe Gly Gln Thr
165 170 175
Gly Asp Ala Asp Ser Val Pro Asp Pro Gln Pro Leu Gly Gln Pro Pro
180 185 190
Ala Ala Pro Ser Gly Leu Gly Ser Thr Thr Met Ala Thr Gly Ser Gly
195 200 205
Ala Pro Met Ala Asp Asn Asn Glu Gly Ala Asp Gly Val Gly Asn Ser
210 215 220
Ser Gly Asn Trp His Cys Asp Ser Gln Trp Leu Gly Asp Arg Val Ile
225 230 235 240
Thr Thr Ser Thr Arg Thr Trp Ala Gln Pro Thr Tyr Asn Asn His Leu
245 250 255
Tyr Lys Gln Ile Ser Ser Gln Ser Gly Ala Ser Asn Asp Asn His Tyr
260 265 270
Phe Gly Tyr Ser Thr Pro Trp Gly Tyr Phe Asp Phe Asn Arg Phe His
275 280 285
Cys His Phe Ser Pro Arg Asp Trp Gln Arg Leu Ile Asn Asn Asn Trp
290 295 300
Gly Phe Arg Pro Lys Arg Leu Asn Phe Lys Leu Phe Asn Ile Gln Val
305 310 315 320
Lys Glu Val Thr Gln Asn Asp Gly Thr Thr Thr Ile Ala Asn Asn Leu
325 330 335
Thr Ser Thr Val Gln Val Phe Thr Asp Ser Glu Tyr Gln Leu Pro Tyr
340 345 350
Val Leu Gly Ser Ala His Gln Gly Cys Leu Pro Pro Phe Pro Ala Asp
355 360 365
Val Phe Met Val Pro Gln Tyr Gly Tyr Leu Thr Leu Asn Asn Gly Ser
370 375 380
Gln Ala Val Gly Arg Ser Ser Phe Tyr Cys Leu Glu Tyr Phe Pro Ser
385 390 395 400
Gln Met Leu Arg Thr Gly Asn Asn Phe Gln Phe Ser Tyr Thr Phe Glu
405 410 415
Asp Val Pro Phe His Ser Ser Tyr Ala His Ser Gln Ser Leu Asp Arg
420 425 430
Leu Met Asn Pro Leu Ile Asp Gln Tyr Leu Tyr Tyr Leu Asn Lys Thr
435 440 445
Gln Ser Asn Ser Gly Ala Leu Gln Gln Ser Arg Leu Leu Phe Ser Gln
450 455 460
Ala Gly Pro Thr Ser Met Ser Leu Gln Ala Lys Asn Trp Leu Pro Gly
465 470 475 480
Pro Cys Tyr Arg Gln Gln Arg Leu Ser Lys Gln Ala Asn Asp Asn Asn
485 490 495
Asn Ser Asn Phe Pro Trp Thr Ala Ala Thr Lys Tyr His Leu Asn Gly
500 505 510
Arg Asp Ser Leu Val Asn Pro Gly Pro Ala Met Ala Ser His Lys Asp
515 520 525
Asp Glu Glu Lys Phe Phe Pro Met His Gly Thr Leu Ile Phe Gly Lys
530 535 540
Gln Gly Thr Asn Ala Asn Asp Ala Asp Leu Asp Asn Val Met Ile Thr
545 550 555 560
Asp Glu Glu Glu Ile Arg Thr Thr Asn Pro Val Ala Thr Glu Gln Tyr
565 570 575
Gly Tyr Val Ser Asn Asn Leu Gln Asn Ser Asn Thr Gly Pro Thr Thr
580 585 590
Gly Thr Val Asn His Arg Gly Ala Leu Pro Gly Met Val Trp Gln Asp
595 600 605
Arg Asp Val Tyr Leu Gln Gly Pro Ile Trp Ala Lys Ile Pro His Thr
610 615 620
Asp Gly His Phe His Pro Ser Pro Leu Met Gly Gly Phe Gly Leu Lys
625 630 635 640
His Pro Pro Pro Gln Ile Met Ile Lys Asn Thr Pro Val Pro Ala Asn
645 650 655
Pro Pro Thr Asn Phe Ser Ser Ala Lys Phe Ala Ser Phe Ile Thr Gln
660 665 670
Tyr Ser Thr Gly Gln Val Ser Val Glu Ile Glu Trp Glu Leu Gln Lys
675 680 685
Glu Asn Ser Lys Arg Trp Asn Pro Glu Ile Gln Tyr Thr Ser Asn Tyr
690 695 700
Asn Lys Ser Val Asn Val Asp Phe Thr Val Asp Thr Asn Gly Val Tyr
705 710 715 720
Ser Glu Pro Arg Pro Ile Gly Thr Arg Tyr Leu Thr Arg Asn Leu
725 730 735
<210>153
<211>735
<212>PRT
<213>vp1,克隆hu.11
<400>153
Met Ala Ala Asp Gly Tyr Leu Pro Asp Trp Leu Glu Asp Thr Leu Ser
1 5 10 15
Glu Gly Ile Arg Gln Trp Trp Lys Leu Lys Pro Gly Pro Pro Pro Pro
20 25 30
Lys Pro Ala Glu Arg His Gln Asp Asp Ser Arg Gly Leu Val Leu Pro
35 40 45
Gly Tyr Lys Tyr Leu Gly Pro Phe Asn Gly Leu Asp Lys Gly Glu Pro
50 55 60
Val Asn Glu Ala Asp Ala Ala Ala Leu Glu His Asp Lys Ala Tyr Asp
65 70 75 80
Arg Gln Leu Asp Ser Gly Asp Asn Pro Tyr Leu Lys Tyr Asn His Ala
85 90 95
Asp Ala Glu Phe Gln Glu Arg Leu Lys Glu Asp Thr Ser Phe Gly Gly
100 105 110
Asn Leu Gly Arg Ala Val Phe Gln Ala Lys Lys Arg Val Leu Glu Pro
115 120 125
Leu Gly Leu Val Glu Glu Pro Val Lys Thr Ala Pro Gly Lys Lys Arg
130 135 140
Pro Val Glu His Ser Pro Val Glu Pro Asp Ser Ser Ser Gly Thr Gly
145 150 155 160
Lys Ala Gly His Gln Pro Ala Arg Lys Arg Leu Asn Phe Gly Gln Thr
165 170 175
Gly Asp Ala Asp Ser Val Pro Asp Pro Gln Pro Leu Gly Gln Pro Pro
180 185 190
Ala Ala Pro Thr Ser Leu Gly Ser Thr Thr Met Ala Thr Gly Ser Gly
195 200 205
Ala Pro Met Ala Asp Asn Asn Glu Gly Ala Asp Gly Val Gly Asn Ser
210 215 220
Ser Gly Asn Trp His Cys Asp Ser Gln Trp Leu Gly Asp Arg Val Ile
225 230 235 240
Thr Thr Ser Thr Arg Thr Trp Ala Leu Pro Thr Tyr Asn Asn His Leu
245 250 255
Tyr Lys Gln Ile Ser Ser Gln Ser Gly Ala Ser Asn Asp Asn His Tyr
260 265 270
Phe Gly Tyr Ser Thr Pro Trp Gly Tyr Phe Asp Phe Asn Arg Phe His
275 280 285
Cys His Phe Ser Pro Arg Asp Trp Gln Arg Leu Ile Asn Asn Asn Trp
290 295 300
Gly Phe Arg Pro Lys Arg Leu Asn Phe Lys Leu Phe Asn Ile Gln Val
305 310 315 320
Lys Glu Val Thr Gln Asn Asp Gly Thr Thr Thr Ile Ala Asn Asn Leu
325 330 335
Thr Ser Thr Val Gln Val Phe Thr Asp Ser Glu Tyr Gln Leu Pro Tyr
340 345 350
Val Leu Gly Ser Ala His Gln Gly Cys Leu Pro Pro Phe Pro Ala Asp
355 360 365
Val Phe Met Val Pro Gln Tyr Gly Tyr Leu Thr Leu Asn Asn Gly Ser
370 375 380
Gln Ala Val Gly Arg Ser Ser Phe Tyr Cys Leu Glu Tyr Phe Pro Ser
385 390 395 400
Gln Met Leu Arg Thr Gly Asn Asn Phe Thr Phe Ser Tyr Thr Phe Glu
405 410 415
Asp Val Pro Phe His Ser Ser Tyr Ala His Ser Gln Ser Leu Asp Arg
420 425 430
Leu Met Asn Pro Leu Ile Asp Gln Tyr Leu Tyr Tyr Leu Asn Arg Thr
435 440 445
Gln Ser Asn Ser Gly Thr Leu Gln Gln Ser Arg Leu Leu Phe Ser Gln
450 455 460
Ala Gly Pro Thr Ser Met Ser Leu Gln Ala Lys Asn Trp Leu Pro Gly
465 470 475 480
Pro Cys Tyr Arg Gln Gln Arg Leu Ser Lys Gln Ala Asn Asp Asn Asn
485 490 495
Asn Ser Asn Phe Pro Trp Thr Ala Ala Thr Lys Tyr Arg Leu Asn Gly
500 505 510
Arg Asp Ser Leu Val Asn Pro Gly Pro Ala Met Ala Ser His Lys Asp
515 520 525
Asp Glu Glu Lys Phe Phe Pro Met His Gly Thr Leu Ile Phe Gly Lys
530 535 540
Gln Gly Thr Asn Ala Asn Asp Ala Asp Leu Glu His Val Met Ile Thr
545 550 555 560
Asp Glu Glu Glu Ile Arg Thr Thr Asn Pro Val Ala Thr Glu Gln Tyr
565 570 575
Gly Asn Val Ser Asn Asn Leu Gln Asn Ser Asn Thr Gly Pro Thr Thr
580 585 590
Glu Asn Val Asn His Gln Gly Ala Leu Pro Gly Met Val Trp Gln Asp
595 600 605
Arg Asp Val Tyr Leu Gln Gly Pro Ile Trp Ala Lys Ile Pro His Thr
610 615 620
Asp Gly His Phe His Pro Ser Pro Leu Met Gly Gly Phe Gly Leu Lys
625 630 635 640
His Pro Pro Pro Gln Ile Met Ile Lys Asn Thr Pro Val Pro Ala Asn
645 650 655
Pro Pro Thr Asn Phe Ser Ser Ala Lys Phe Ala Ser Phe Ile Thr Gln
660 665 670
Tyr Ser Thr Gly Gln Val Ser Val Glu Ile Glu Trp Glu Leu Gln Lys
675 680 685
Glu Asn Ser Lys Arg Trp Asn Pro Glu Ile Gln Tyr Thr Ser Asn Tyr
690 695 700
Asn Lys Ser Val Asn Val Asp Phe Thr Val Asp Thr Asn Gly Val Tyr
705 710 715 720
Ser Glu Pro Arg Pro Ile Gly Thr Arg Tyr Leu Thr Arg Asn Leu
725 730 735
<210>154
<211>735
<212>PRT
<213>vp1,克隆hu.12
<400>154
Met Ala Ala Asp Gly Tyr Leu Pro Asp Trp Leu Glu Asp Thr Leu Ser
1 5 10 15
Glu Gly Ile Arg Gln Trp Trp Lys Leu Lys Pro Gly Pro Pro Pro Pro
20 25 30
Lys Pro Ala Glu Arg His Gln Asp Asp Ser Arg Gly Leu Val Leu Pro
35 40 45
Gly Tyr Lys Tyr Leu Gly Pro Phe Asn Gly Leu Asp Lys Gly Glu Pro
50 55 60
Val Asn Glu Ala Asp Ala Ala Ala Leu Glu His Asp Lys Ala Tyr Asp
65 70 75 80
Arg Gln Leu Asp Ser Gly Asp Asn Pro Tyr Leu Lys Tyr Asn His Ala
85 90 95
Asp Ala Glu Phe Gln Glu Arg Leu Lys Glu Asp Thr Ser Phe Gly Gly
100 105 110
Asn Leu Gly Arg Ala Val Phe Gln Ala Lys Lys Arg Val Leu Glu Pro
115 120 125
Leu Gly Leu Val Glu Glu Pro Val Lys Thr Ala Pro Gly Lys Lys Arg
130 135 140
Pro Val Glu His Ser Pro Val Glu Pro Asp Ser Ser Ser Gly Thr Gly
145 150 155 160
Lys Ala Gly His Gln Pro Ala Arg Lys Arg Leu Asn Phe Gly Gln Thr
165 170 175
Gly Asp Ala Asp Ser Val Pro Asp Pro Gln Pro Leu Gly Gln Pro Pro
180 185 190
Ala Ala Pro Thr Ser Leu Gly Ser Thr Thr Met Ala Thr Gly Ser Gly
195 200 205
Ala Pro Met Ala Asp Asn Asn Glu Gly Ala Asp Gly Val Gly Asn Ser
210 215 220
Ser Gly Asn Trp His Cys Asp Ser Gln Trp Leu Gly Asp Arg Val Ile
225 230 235 240
Thr Thr Ser Thr Arg Thr Trp Ala Leu Pro Thr Tyr Asn Asn His Leu
245 250 255
Tyr Lys Gln Ile Ser Ser Gln Ser Gly Ala Ser Asn Asp Asn His Tyr
260 265 270
Phe Gly Tyr Ser Thr Pro Trp Gly Tyr Phe Asp Phe Asn Arg Phe His
275 280 285
Cys His Phe Ser Pro Arg Asp Trp Gln Arg Leu Ile Asn Asn Asn Trp
290 295 300
Gly Phe Arg Pro Lys Arg Leu Asn Phe Lys Leu Phe Asn Ile Gln Val
305 310 315 320
Lys Glu Val Thr Gln Asn Asp Gly Thr Thr Thr Ile Ala Asn Asn Leu
325 330 335
Thr Ser Thr Val Gln Val Phe Thr Asp Ser Glu Tyr Gln Leu Pro Tyr
340 345 350
Val Leu Gly Ser Ala His Gln Gly Cys Leu Pro Pro Phe Pro Ala Asp
355 360 365
Val Phe Met Val Pro Gln Tyr Gly Tyr Leu Thr Leu Asn Asn Gly Ser
370 375 380
Gln Ala Val Gly Arg Pro Ser Phe Tyr Cys Leu Glu Tyr Phe Pro Ser
385 390 395 400
Gln Met Leu Arg Thr Gly Asn Asn Phe Thr Phe Ser Tyr Thr Phe Glu
405 410 415
Asp Val Pro Phe His Ser Ser Tyr Ala His Ser Gln Ser Leu Asp Arg
420 425 430
Leu Met Asn Pro Leu Ile Asp Gln Tyr Leu Tyr Tyr Leu Asn Arg Thr
435 440 445
Gln Ser Asn Ser Gly Thr Leu Gln Gln Ser Arg Leu Leu Phe Ser Gln
450 455 460
Ala Gly Pro Thr Ser Met Ser Leu Gln Ala Lys Asn Trp Leu Pro Gly
465 470 475 480
Pro Cys Tyr Arg Gln Gln Arg Leu Ser Lys Gln Ala Asn Asp Asn Asn
485 490 495
Asr Ser Asn Phe Pro Trp Thr Ala Ala Thr Lys Tyr His Leu Asn Gly
500 505 510
Arg Asp Ser Leu Val Asn Pro Gly Pro Ala Met Ala Ser His Lys Asp
515 520 525
Asp Glu Glu Lys Phe Phe Pro Met His Gly Thr Leu Ile Phe Gly Lys
530 535 540
Gln Gly Thr Asn Ala Asn Asp Ala Asp Leu Glu His Val Met Ile Thr
545 550 555 560
Asp Glu Glu Glu Ile Arg Thr Thr Asn Pro Val Ala Thr Glu Gln Tyr
565 570 575
Gly Asn Val Ser Asn Asn Leu Gln Asn Ser Asn Thr Gly Pro Thr Thr
580 585 590
Glu Asn Val Asn His Gln Gly Ala Leu Pro Gly Met Val Trp Gln Asp
595 600 605
Arg Asp Val Tyr Leu Gln Gly Pro Ile Trp Ala Lys Ile Pro His Thr
610 615 620
Asp Gly His Phe His Pro Ser Pro Leu Met Gly Gly Phe Gly Leu Lys
625 630 635 640
His Pro Pro Pro Gln Ile Met Ile Lys Asn Thr Pro Val Pro Ala Asn
645 650 655
Pro Pro Thr Asn Phe Ser Ser Ala Lys Phe Ala Ser Phe Ile Thr Gln
660 665 670
Tyr Ser Thr Gly Gln Val Ser Val Glu Ile Glu Trp Glu Leu Gln Lys
675 680 685
Glu Asn Ser Lys Arg Trp Asn Pro Glu Ile Gln Tyr Thr Ser Asn Tyr
690 695 700
Asn Lys Ser Val Asn Val Asp Phe Thr Val Asp Thr Asn Gly Val Tyr
705 710 715 720
Ser Glu Pro Arg Pro Ile Gly Thr Arg Tyr Leu Thr Arg Asn Leu
725 730 735
<210>155
<211>735
<212>PRT
<213>vp1,克隆hu.9
<400>155
Met Ala Ala Asp Gly Tyr Leu Pro Asp Trp Leu Glu Asp Thr Leu Ser
1 5 10 15
Glu Gly Ile Arg Gln Trp Trp Lys Leu Lys Pro Gly Pro Pro Pro Pro
20 25 30
Lys Pro Ala Glu Arg His Gln Asp Asn Ser Arg Gly Leu Val Leu Pro
35 40 45
Gly Tyr Lys Tyr Leu Gly Pro Ser Asn Gly Leu Asp Lys Gly Glu Pro
50 55 60
Val Asn Glu Ala Asp Ala Ala Ala Leu Glu His Asp Lys Ala Tyr Asp
65 70 75 80
Arg Gln Leu Asp Ser Gly Asp Asn Pro Tyr Leu Lys Tyr Asn His Ala
85 90 95
Asp Ala Glu Phe Gln Glu Arg Leu Lys Glu Asp Thr Ser Phe Gly Gly
100 105 110
Asn Leu Gly Arg Ala Val Phe Gln Ala Lys Lys Arg Val Leu Glu Pro
115 120 125
Leu Gly Leu Val Glu Glu Pro Val Lys Thr Ala Pro Gly Lys Lys Arg
130 135 140
Pro Val Glu His Ser Pro Val Glu Pro Asp Ser Ser Ser Gly Thr Gly
145 150 155 160
Lys Ala Gly His Gln Pro Ala Arg Lys Arg Leu Asn Phe Gly Gln Thr
165 170 175
Gly Asp Ala Asp Ser Val Pro Asp Pro Gln Pro Leu Gly Gln Pro Pro
180 185 190
Ala Ala Pro Thr Ser Leu Gly Ser Thr Thr Met Ala Thr Gly Ser Gly
195 200 205
Ala Pro Met Ala Asp Asn Asn Glu Gly Ala Asp Gly Val Gly Asn Ser
210 215 220
Ser Gly Asn Trp His Cys Asp Ser Gln Trp Leu Gly Asp Arg Val Ile
225 230 235 240
Thr Thr Ser Thr Arg Thr Trp Ala Leu Pro Thr Tyr Asn Asn His Leu
245 250 255
Tyr Lys Gln Ile Ser Ser Gln Ser Gly Ala Ser Asn Asp Asn His Tyr
260 265 270
Phe Gly Cys Ser Thr Pro Trp Gly Tyr Phe Asp Phe Asn Arg Phe His
275 280 285
Cys His Phe Ser Pro Arg Asp Trp Gln Arg Leu Ile Asn Asn Asn Trp
290 295 300
Gly Phe Arg Pro Lys Arg Leu Asn Phe Lys Leu Phe Asn Ile Gln Val
305 310 315 320
Lys Glu Val Thr Gln Asn Asp Gly Thr Thr Thr Ile Ala Asn Asn Leu
325 330 335
Thr Ser Thr Val Gln Val Phe Thr Asp Ser Glu Tyr Pro Leu Pro Tyr
340 345 350
Val Leu Gly Ser Ala His Gln Gly Cys Leu Pro Pro Phe Pro Ala Asp
355 360 365
Val Phe Met Val Pro Gln Tyr Gly Tyr Leu Thr Leu Asn Asn Gly Ser
370 375 380
Gln Ala Val Gly Arg Ser Ser Phe Tyr Cys Leu Glu Tyr Phe Pro Ser
385 390 395 400
Gln Met Leu Arg Thr Gly Asn Asn Phe Thr Phe Ser Tyr Thr Phe Glu
405 410 415
Asp Val Pro Phe His Ser Ser Tyr Ala His Ser Gln Ser Leu Asp Arg
420 425 430
Leu Met Asn Pro Leu Ile Asp Gln Tyr Leu Tyr Tyr Leu Asn Arg Thr
435 440 445
Gln Ser Asn Ser Gly Thr Leu Gln Gln Ser Arg Leu Leu Phe Ser Gln
450 455 460
Ala Gly Pro Thr Ser Met Ser Leu Gln Ala Lys Asn Trp Leu Pro Gly
465 470 475 480
Pro Cys Tyr Arg Gln Gln Arg Leu Ser Lys Gln Ala Asn Asp Asn Asn
485 490 495
Asn Ser Asn Phe Pro Trp Thr Ala Ala Thr Lys Tyr His Leu Asn Gly
500 505 510
Arg Asp Ser Leu Val Asn Pro Gly Pro Ala Met Ala Ser His Lys Asp
515 520 525
Asp Glu Glu Lys Phe Phe Pro Met His Gly Thr Leu Ile Phe Gly Lys
530 535 540
Gln Gly Thr Asn Ala Asn Asp Ala Asp Leu Glu His Val Met Ile Thr
545 550 555 560
Asp Glu Glu Glu Ile Arg Thr Thr Asn Pro Val Ala Thr Glu Gln Tyr
565 570 575
Gly Asn Val Ser Asn Asn Leu Gln Asn Ser Asn Thr Gly Pro Thr Thr
580 585 590
Glu Asn Val Asn His Gln Gly Ala Leu Pro Gly Met Val Trp Gln Asp
595 600 605
Arg Asp Val Tyr Leu Gln Gly Pro Ile Trp Ala Lys Ile Pro His Thr
610 615 620
Asp Gly His Phe His Pro Ser Pro Leu Met Gly Gly Phe Gly Leu Lys
625 630 635 640
His Pro Pro Pro Gln Ile Met Ile Lys Asn Thr Pro Val Pro Ala Asn
645 650 655
Pro Pro Thr Asn Phe Ser Ser Ala Lys Phe Ala Ser Phe Ile Thr Gln
660 665 670
Tyr Ser Thr Gly Gln Val Ser Val Glu Ile Glu Trp Glu Leu Gln Lys
675 680 685
Glu Asn Ser Lys Arg Trp Asn Pro Glu Ile Gln Tyr Thr Ser Asn Tyr
690 695 700
Asn Lys Ser Val Asn Val Asp Phe Thr Val Asp Thr Asn Gly Val Tyr
705 710 715 720
Ser Glu Pro Cys Pro Ile Gly Thr Arg Tyr Leu Thr Arg Asn Leu
725 730 735
<210>156
<211>735
<212>PRT
<213>vp1,克隆hu.10
<400>156
Met Ala Ala Asp Gly Tyr Leu Pro Asp Trp Leu Glu Asp Thr Leu Ser
1 5 10 15
Glu Gly Ile Arg Gln Trp Trp Lys Leu Lys Pro Gly Pro Pro Pro Pro
20 25 30
Lys Leu Ala Glu Arg His Gln Asp Asp Ser Arg Gly Leu Val Leu Pro
35 40 45
Gly Tyr Lys Tyr Leu Gly Pro Phe Asn Gly Leu Asp Lys Gly Glu Pro
50 55 60
Val Asn Glu Ala Asp Ala Ala Ala Leu Glu His Asp Lys Ala Tyr Asp
65 70 75 80
Arg Gln Leu Asp Ser Gly Asp Asn Pro Tyr Leu Lys Tyr Asn His Ala
85 90 95
Asp Ala Glu Phe Gln Glu Arg Leu Lys Glu Asp Thr Ser Phe Gly Gly
100 105 110
Asn Leu Gly Arg Ala Val Phe Gln Ala Lys Lys Arg Val Leu Glu Pro
115 120 125
Leu Gly Leu Val Glu Glu Pro Val Lys Thr Ala Pro Gly Lys Lys Arg
130 135 140
Pro Val Glu His Ser Pro Val Glu Pro Asp Ser Ser Ser Gly Thr Gly
145 150 155 160
Lys Ala Gly His Gln Pro Ala Arg Lys Arg Leu Asn Phe Gly Gln Thr
165 170 175
Gly Asp Ala Asp Ser Val Pro Asp Pro Gln Pro Leu Gly Gln Pro Pro
180 185 190
Ala Ala Pro Thr Ser Leu Gly Ser Thr Thr Met Ala Thr Gly Ser Gly
195 200 205
Ala Pro Met Ala Asp Asn Asn Glu Gly Ala Asp Gly Val Gly Asn Ser
210 215 220
Ser Gly Asn Trp His Cys Asp Ser Gln Trp Leu Gly Asp Arg Val Ile
225 230 235 240
Thr Thr Ser Thr Arg Thr Trp Ala Leu Pro Thr Tyr Asn Asn His Leu
245 250 255
Tyr Lys Gln Ile Ser Ser Gln Ser Gly Ala Ser Asn Asp Asn His Tyr
260 265 270
Phe Gly Tyr Ser Thr Pro Trp Gly Tyr Phe Asp Phe Asn Arg Phe His
275 280 285
Cys His Phe Ser Pro Arg Asp Trp Gln Arg Leu Ile Asn Asn Asn Trp
290 295 300
Gly Phe Arg Pro Lys Arg Leu Asn Phe Lys Leu Phe Asn Ile Gln Val
305 310 315 320
Lys Glu Val Thr Gln Asn Asp Gly Thr Thr Thr Ile Ala Asn Asn Leu
325 330 335
Thr Ser Thr Val Gln Val Phe Thr Asp Ser Glu Tyr Gln Leu Pro Tyr
340 345 350
Val Leu Gly Ser Ala His Gln Gly Cys Leu Pro Pro Phe Pro Ala Asp
355 360 365
Val Phe Thr Val Pro Gln Tyr Gly Tyr Leu Thr Leu Asn Asn Gly Ser
370 375 380
Gln Ala Val Gly Arg Ser Ser Phe Tyr Cys Leu Glu Tyr Phe Pro Ser
385 390 395 400
Gln Met Leu Arg Thr Gly Asn Asn Leu Thr Phe Ser Tyr Thr Phe Glu
405 410 415
Asp Val Pro Phe His Ser Ser Tyr Ala His Ser Gln Ser Leu Asp Arg
420 425 430
Leu Met Asn Pro Leu Ile Asp Gln Tyr Leu Tyr Tyr Leu Asn Arg Thr
435 440 445
Gln Ser Asn Ser Gly Thr Leu Gln Gln Ser Arg Leu Leu Phe Ser Gln
450 455 460
Ala Gly Pro Thr Ser Met Ser Leu Gln Ala Lys Asn Trp Leu Pro Gly
465 470 475 480
Pro Cys Tyr Arg Gln Gln Arg Leu Ser Lys Gln Ala Asn Asp Asn Asn
485 490 495
Asn Ser Asn Phe Pro Trp Thr Ala Ala Thr Lys Tyr His Leu Asn Gly
500 505 510
Arg Asp Ser Leu Val Asn Pro Gly Pro Ala Met Ala Ser His Lys Asp
515 520 525
Asp Glu Glu Lys Phe Phe Pro Met His Gly Thr Leu Ile Phe Gly Lys
530 535 540
Gln Gly Thr Asn Ala Asn Asp Ala Asp Leu Glu His Val Met Ile Thr
545 550 555 560
Asp Glu Glu Glu Ile Arg Thr Thr Asn Pro Val Ala Thr Glu Gln Tyr
565 570 575
Gly Asn Val Ser Asn Asn Leu Gln Asn Ser Asn Thr Gly Pro Thr Thr
580 585 590
Glu Asn Val Asn His Gln Gly Ala Leu Pro Gly Met Val Trp Gln Asp
595 600 605
Arg Asp Val Tyr Leu Gln Gly Pro Ile Trp Ala Lys Ile Pro His Thr
610 615 620
Asp Gly His Phe His Pro Ser Pro Leu Met Gly Gly Phe Gly Leu Lys
625 630 635 640
His Pro Pro Pro Gln Ile Met Ile Lys Asn Thr Pro Val Pro Ala Asn
645 650 655
Pro Pro Thr Asn Tyr Ser Ser Ala Lys Phe Ala Ser Phe Ile Thr Gln
660 665 670
Tyr Ser Thr Gly Gln Val Ser Val Glu Ile Glu Trp Glu Leu Arg Lys
675 680 685
Glu Asn Ser Lys Arg Trp Asn Pro Glu Ile Gln Tyr Thr Ser Asn Tyr
690 695 700
Asn Lys Ser Val Asn Val Asp Phe Thr Val Asp Thr Asn Gly Val Tyr
705 710 715 720
Ser Glu Pro Arg Pro Ile Gly Thr Arg Tyr Leu Thr Arg Asn Leu
725 730 735
<210>157
<211>736
<212>PRT
<213>vp1,克隆hu.48
<400>157
Met Ala Ala Asp Gly Tyr Leu Pro Asp Trp Leu Glu Asp Asn Leu Ser
1 5 10 15
Glu Gly Ile Arg Glu Trp Trp Asp Leu Lys Pro Gly Ala Pro Lys Pro
20 25 30
Lys Ala Asn Gln Gln Lys Gln Asp Asp Gly Arg Gly Leu Val Leu Pro
35 40 45
Gly Tyr Lys Tyr Leu Gly Pro Phe Asn Gly Leu Asp Lys Gly Glu Pro
50 55 60
Val Asn Ala Ala Asp Ala Ala Ala Leu Glu His Asp Lys Ala Tyr Asp
65 70 75 80
Gln Gln Leu Lys Ala Gly Asp Asn Pro Tyr Leu Arg Tyr Asn His Ala
85 90 95
Asp Ala Glu Phe Gln Glu Arg Leu Gln Glu Asp Thr Ser Phe Gly Gly
100 105 110
Asn Leu Gly Arg Ala Val Phe Gln Ala Lys Lys Arg Val Leu Glu Pro
115 120 125
Leu Gly Leu Val Glu Glu Gly Ala Lys Thr Ala Pro Gly Lys Lys Arg
130 135 140
Pro Val Glu Gln Ser Pro Gln Glu Pro Asp Ser Ser Ser Gly Ile Gly
145 150 155 160
Lys Thr Gly Gln Gln Pro Ala Lys Lys Arg Leu Asn Phe Gly Gln Thr
165 170 175
Gly Asp Ser Glu Ser Val Pro Asp Pro Gln Pro Leu Gly Glu Pro Pro
180 185 190
Ala Thr Pro Ala Ala Val Gly Pro Thr Thr Met Ala Ser Gly Gly Gly
195 200 205
Ala Pro Met Ala Asp Asn Asn Glu Gly Ala Asp Gly Val Gly Asn Ala
210 215 220
Ser Gly Asn Trp His Cys Asp Ser Thr Trp Leu Gly Asp Arg Val Ile
225 230 235 240
Thr Thr Ser Thr Arg Thr Trp Ala Leu Pro Thr Tyr Asn Asn His Leu
245 250 255
Tyr Lys Gln Ile Ser Ser Thr Ser Thr Gly Ala Ser Asn Asp Asn His
260 265 270
Tyr Phe Gly Tyr Gly Thr Pro Trp Gly Tyr Phe Asp Phe Asn Arg Phe
275 280 285
His Cys His Phe Ser Pro Arg Asp Trp Gln Arg Leu Ile Asn Asn Asn
290 295 300
Trp Gly Phe Arg Pro Lys Arg Leu Asn Phe Lys Leu Phe Asn Ile Gln
305 310 315 320
Val Glu Glu Val Thr Thr Asn Asp Gly Val Thr Thr Ile Ala Asn Asn
325 330 335
Leu Thr Ser Thr Val Gln Val Phe Ser Asp Ser Glu Tyr Gln Leu Pro
340 345 350
Tyr Val Leu Gly Ser Ala His Gln Gly Cys Leu Pro Pro Phe Pro Ala
355 360 365
Asp Val Phe Met Ile Pro Gln Tyr Gly Tyr Leu Thr Leu Asn Asn Gly
370 375 380
Ser Gln Ala Val Gly Arg Ser Ser Phe Tyr Cys Leu Glu Tyr Phe Pro
385 390 395 400
Ser Gln Met Leu Arg Thr Gly Asn Asn Phe Thr Phe Ser Tyr Thr Phe
405 410 415
Glu Glu Val Pro Phe His Ser Ser Tyr Ala His Ser Gln Ser Leu Asp
420 425 430
Arg Leu Mer Asn Pro Leu Ile Asp Gln Tyr Leu Tyr Tyr Leu Asn Arg
435 440 445
Thr Gln Asn Gln Ser Gly Ser Ala Gln Asn Lys Asp Leu Leu Phe Ser
450 455 460
Arg Gly Ser Pro Ala Gly Met Ser Val Gln Pro Lys Asn Trp Leu Pro
465 470 475 480
Gly Pro Cys Tyr Arg Gln Gln Arg Val Ser Lys Thr Lys Thr Asp Asn
485 490 495
Asn Asn Ser Asn Phe Thr Trp Thr Gly Ala Ser Lys Tyr Asn Leu Asn
500 505 510
Gly Arg Glu Ser Ile Ile Asn Pro Gly Thr Ala Val Ala Ser His Lys
515 520 525
Asp Asp Glu Asp Lys Phe Phe Pro Met Ser Gly Val Met Ile Phe Gly
530 535 540
Lys Glu Ser Ala Gly Ala Ser Ser Thr Ala Leu Asp Asn Val Met Ile
545 550 555 560
Thr Asp Glu Glu Glu Ile Lys Ala Thr Asn Pro Val Ala Thr Glu Arg
565 570 575
Phe Gly Thr Val Ala Val Asn Phe Gln Ser Ser Ser Thr Asp Pro Ala
580 585 590
Thr Gly Asp Val His Ala Met Gly Ala Leu Pro Gly Met Val Trp Gln
595 600 605
Asp Arg Asp Val Tyr Leu Gln Gly Pro Ile Trp Ala Lys Ile Pro His
610 615 620
Thr Asp Gly His Phe His Pro Ser Pro Leu Met Gly Gly Phe Gly Leu
625 630 635 640
Lys Asn Pro Pro Pro Gln Ile Leu Ile Lys Asn Thr Pro Val Pro Ala
645 650 655
Asn Pro Pro Ala Glu Phe Ser Ala Thr Lys Phe Ala Ser Phe Ile Thr
660 665 670
Gln Tyr Ser Thr Gly Gln Val Ser Val Glu Ile Glu Trp Glu Leu Gln
675 680 685
Lys Glu Asn Ser Lys Arg Trp Asn Pro Glu Val Gln Tyr Thr Ser Asn
690 695 700
Tyr Ala Lys Ser Ala Asn Val Asp Phe Thr Val Asp Asn Asn Gly Leu
705 710 715 720
Tyr Thr Glu Pro Arg Pro Ile Gly Thr Arg Tyr Leu Thr Arg Pro Leu
725 730 735
<210>158
<211>736
<212>PRT
<213>vp1,克隆hu.44
<400>158
Met Ala Ala Asp Gly Tyr Leu Pro Asp Trp Leu Glu Asp Thr Leu Ser
1 5 10 15
Glu Gly Ile Arg Gln Trp Trp Lys Leu Arg Pro Gly Pro Pro Pro Pro
20 25 30
Lys Pro Ala Glu Arg His Lys Asp Asp Ser Arg Gly Leu Val Leu Pro
35 40 45
Gly Tyr Lys Tyr Leu Gly Pro Phe Asn Gly Leu Asp Lys Gly Glu Pro
50 55 60
Val Asn Glu Ala Asp Ala Ala Ala Leu Glu His Asp Lys Ala Tyr Asp
65 70 75 80
Arg Gln Leu Asp Ser Gly Asp Asn Pro Tyr Leu Lys Tyr Asn His Ala
85 90 95
Asp Ala Glu Phe Gln Glu Arg Leu Lys Glu Asp Thr Ser Phe Gly Gly
100 105 110
Asn Leu Gly Arg Ala Val Phe Gln Ala Lys Lys Arg Val Leu Glu Pro
115 120 125
Leu Gly Leu Val Glu Glu Gly Ala Glu Thr Ala Pro Gly Lys Lys Arg
130 135 140
Pro Val Glu Gln Ser Pro Gln Gly Pro Asp Ser Ser Ser Gly Ile Gly
145 150 155 160
Lys Thr Gly Gln Gln Pro Ala Lys Lys Arg Leu Asn Phe Gly Gln Thr
165 170 175
Gly Asp Ser Glu Ser Val Pro Asp Pro Gln Pro Leu Gly Glu Pro Pro
180 185 190
Ala Thr Pro Ala Ala Val Gly Pro Thr Thr Met Ala Ser Gly Gly Gly
195 200 205
Ala Pro Met Ala Asp Asn Asn Glu Gly Ala Asp Gly Val Gly Asn Ala
210 215 220
Ser Gly Asn Trp His Cys Asp Ser Thr Trp Leu Gly Asp Arg Val Ile
225 230 235 240
Thr Thr Ser Thr Arg Thr Trp Ala Leu Pro Thr Tyr Asn Asn His Leu
245 250 255
Tyr Lys Gln Ile Ser Ser Ala Ser Thr Gly Ala Ser Asn Asp Asn His
260 265 270
Tyr Phe Gly Tyr Ser Thr Pro Trp Gly Tyr Phe Asp Phe Asn Arg Phe
275 280 285
His Cys His Phe Ser Pro Arg Asp Trp Gln Arg Leu Ile Asn Asn Asn
290 295 300
Trp Gly Phe Arg Pro Lys Arg Leu Asn Phe Lys Leu Phe Asn Ile Gln
305 310 315 320
Val Lys Glu Val Thr Thr Asn Asp Gly Val Thr Thr Ile Ala Asn Asn
325 330 335
Leu Thr Ser Thr Val Gln Val Phe Ser Asp Ser Glu Tyr Gln Leu Pro
340 345 350
Tyr Val Leu Gly Ser Ala His Gln Gly Cys Leu Pro Pro Phe Pro Ala
355 360 365
Asp Val Phe Met Ile Pro Gln Tyr Gly Tyr Leu Thr Leu Asn Asn Gly
370 375 380
Ser Gln Ala Val Gly Arg Ser Ser Phe Tyr Cys Leu Glu Tyr Phe Pro
385 390 395 400
Ser Gln Met Leu Arg Thr Gly Asn Asn Phe Thr Phe Ser Tyr Thr Phe
405 410 415
Glu Glu Val Pro Phe His Ser Ser Tyr Ala His Ser Gln Ser Leu Asp
420 425 430
Arg Leu Met Asn Pro Leu Ile Asp Gln Tyr Leu Tyr Tyr Pro Asn Arg
435 440 445
Thr Gln Asn Gln Ser Gly Ser Ala Gln Asn Lys Asp Leu Leu Phe Ser
450 455 460
Arg Gly Ser Pro Ala Gly Met Ser Val Gln Pro Lys Asn Trp Leu Pro
465 470 475 480
Gly Pro Cys Tyr Arg Gln Gln Arg Val Ser Lys Thr Lys Thr Asp Asn
485 490 495
Asn Asn Ser Asn Phe Thr Trp Thr Gly Ala Ser Lys Tyr Asn Leu Asn
500 505 510
Gly Arg Glu Ser Ile Ile Asn Pro Gly Thr Ala Met Ala Ser His Lys
515 520 525
Asp Asp Glu Asp Lys Phe Phe Pro Met Ser Gly Val Met Ile Phe Gly
530 535 540
Lys Glu Ser Ala Gly Ala Ser Asn Thr Ala Leu Asp Asn Val Met Ile
545 550 555 560
Thr Asp Glu Glu Glu Ile Lys Ala Thr Asn Pro Val Ala Thr Glu Arg
565 570 575
Phe Gly Thr Val Ala Val Asn Phe Gln Ser Ser Ser Thr Asp Pro Ala
580 585 590
Thr Gly Asp Val His Ala Met Gly Ala Leu Pro Gly Met Val Trp Gln
595 600 605
Gly Arg Asp Val Tyr Leu Gln Gly Pro Ile Trp Ala Lys Ile Pro His
610 615 620
Thr Asp Gly His Phe His Pro Ser Pro Leu Met Gly Gly Phe Gly Leu
625 630 635 640
Lys Asn Pro Pro Pro Gln Ile Leu Ile Lys Asn Thr Pro Val Pro Ala
645 650 655
Asn Pro Pro Ala Glu Phe Ser Ala Thr Lys Phe Ala Ser Phe Ile Thr
660 665 670
Gln Tyr Ser Thr Gly Gln Val Ser Val Glu Ile Glu Trp Glu Leu Gln
675 680 685
Lys Glu Asn Ser Lys Arg Trp Asn Pro Glu Val Gln Tyr Thr Ser Asn
690 695 700
Tyr Ala Lys Ser Ala Asn Val Asp Phe Thr Val Asp Asn Asn Gly Leu
705 710 715 720
Tyr Thr Glu Pro Arg Pro Ile Gly Thr Arg Tyr Leu Thr Arg Pro Leu
725 730 735
<210>159
<211>736
<212>PRT
<213>vp1,克隆hu.46
<400>159
Met Ala Ala Asp Gly Tyr Leu Pro Asp Trp Leu Glu Asp Thr Leu Ser
1 5 10 15
Glu Gly Ile Arg Gln Trp Trp Lys Leu Lys Pro Gly Pro Pro Pro Pro
20 25 30
Lys Pro Ala Glu Arg His Lys Asp Asp Ser Arg Gly Leu Val Leu Pro
35 40 45
Gly Tyr Lys Tyr Leu Gly Pro Phe Asn Gly Leu Asp Lys Gly Glu Pro
50 55 60
Val Asn Glu Ala Asp Ala Ala Ala Leu Glu His Asp Lys Ala Tyr Asp
65 70 75 80
Arg Gln Leu Asp Ser Gly Asp Asn Pro Tyr Leu Lys Tyr Asn His Ala
85 90 95
Asp Ala Glu Phe Gln Glu Arg Leu Lys Glu Asp Thr Ser Phe Gly Gly
100 105 110
Asn Leu Gly Arg Ala Val Phe Gln Ala Lys Lys Arg Val Leu Glu Pro
115 120 125
Leu Gly Leu Val Glu Glu Gly Ala Lys Thr Ala Pro Gly Lys Lys Arg
130 135 140
Pro Val Glu Gln Ser Pro Gln Glu Pro Asp Ser Pro Ser Gly Ile Gly
145 150 155 160
Lys Thr Gly Gln Gln Pro Ala Lys Lys Arg Leu Asn Phe Gly Gln Thr
165 170 175
Gly Asp Ser Glu Ser Val Pro Asp Pro Gln Pro Leu Gly Glu Pro Pro
180 185 190
Ala Thr Pro Ala Ala Val Gly Pro Thr Thr Met Ala Ser Gly Gly Gly
195 200 205
Ala Pro Met Ala Asp Asn Asn Glu Gly Ala Asp Gly Val Gly Asn Ala
210 215 220
Ser Gly Asn Trp His Cys Asp Ser Thr Trp Leu Gly Asp Arg Val Ile
225 230 235 240
Thr Thr Ser Thr Arg Thr Trp Ala Leu Pro Thr Tyr Asn Asn His Leu
245 250 255
Tyr Lys Gln Ile Ser Ser Ala Ser Thr Gly Ala Ser Asn Asp Asn His
260 265 270
Tyr Phe Gly Tyr Ser Thr Pro Trp Gly Tyr Phe Asp Phe Asn Arg Phe
275 280 285
His Cys His Phe Ser Pro Arg Asp Trp Gln Arg Leu Ile Asn Asn Asn
290 295 300
Trp Gly Phe Arg Pro Lys Arg Leu Asn Phe Lys Leu Phe Asn Ile Gln
305 310 315 320
Val Lys Glu Val Thr Thr Asn Asp Gly Val Thr Thr Ile Ala Asn Asn
325 330 335
Leu Thr Ser Thr Val Gln Val Phe Ser Asp Ser Glu Tyr Gln Leu Pro
340 345 350
Tyr Val Leu Gly Ser Ala His Gln Gly Arg Leu Pro Pro Phe Pro Ala
355 360 365
Asp Val Phe Met Ile Pro Gln Tyr Gly Tyr Leu Thr Leu Asn Asn Gly
370 375 380
Ser Gln Ala Val Gly Arg Ser Ser Ser Tyr Cys Leu Glu Tyr Phe Pro
385 390 395 400
Ser Gln Met Leu Arg Thr Gly Asn Asn Phe Thr Phe Ser Tyr Thr Phe
405 410 415
Glu Glu Val Pro Leu His Ser Ser Cys Ala His Ser Gln Ser Leu Asp
420 425 430
Arg Leu Met Asn Pro Leu Ile Asp Gln Tyr Leu Tyr Tyr Leu Asn Arg
435 440 445
Thr Gln Asn Gln Ser Gly Ser Ala Gln Asn Arg Asp Leu Leu Phe Ser
450 455 460
Arg Gly Ser Pro Ala Gly Met Ser Val Gln Pro Lys Asn Trp Leu Pro
465 470 475 480
Gly Pro Cys Tyr Arg Gln Gln Arg Val Ser Lys Thr Lys Thr Asp Asn
485 490 495
Asn Asn Ser Asn Phe Thr Trp Thr Gly Ala Ser Lys Tyr Asn Leu Asn
500 505 510
Gly Arg Glu Ser Ile Ile Asn Pro Gly Thr Ala Met Ala Ser His Lys
515 520 525
Asp Asp Glu Asp Lys Phe Phe Pro Met Ser Gly Val Met Ile Phe Gly
530 535 540
Lys Glu Ser Ala Gly Ala Ser Asn Thr Ala Leu Asp Asn Val Met Ile
545 550 555 560
Thr Asp Glu Glu Glu Ile Lys Ala Thr Asn Pro Val Ala Thr Glu Arg
565 570 575
Phe Gly Thr Val Ala Val Asn Phe Gln Ser Ser Ser Thr Asp Pro Ala
580 585 590
Thr Gly Asp Val His Ala Met Gly Ala Leu Pro Gly Met Val Trp Gln
595 600 605
Asp Arg Asp Val Tyr Leu Gln Gly Pro Ile Trp Ala Lys Ile Pro His
610 615 620
Thr Asp Gly His Phe His Pro Ser Pro Leu Met Gly Gly Phe Gly Leu
625 630 635 640
Lys Asn Pro Pro Pro Gln Ile Leu Ile Lys Asn Thr Pro Val Pro Ala
645 650 655
Asn Pro Pro Ala Glu Phe Ser Ala Thr Lys Phe Ala Ser Phe Ile Thr
660 665 670
Gln Tyr Ser Ala Gly Gln Val Ser Val Glu Ile Glu Trp Glu Leu Gln
675 680 685
Lys Glu Asn Ser Lys Arg Trp Asn Pro Glu Val Gln Tyr Thr Ser Asn
690 695 700
Tyr Ala Lys Ser Ala Asn Val Asp Phe Thr Val Asp Asn Asn Gly Leu
705 710 715 720
Tyr Thr Glu Pro Arg Pro Ile Gly Thr Arg Tyr Leu Thr Arg Pro Leu
725 730 735
<210>160
<211>737
<212>PRT
<213>vp1,克隆hu.43
<400>160
Met Ala Ala Asp Gly Tyr Leu Pro Asp Trp Leu Glu Asp Asn Leu Ser
1 5 10 15
Glu Gly Ile Arg Glu Trp Trp Asp Leu Lys Pro Gly Ala Pro Lys Pro
20 25 30
Lys Ala Asn Gln Gln Lys Gln Asp Asp Gly Arg Gly Leu Val Leu Pro
35 40 45
Gly Tyr Lys Tyr Leu Gly Pro Phe Asn Gly Leu Asp Lys Gly Glu Pro
50 55 60
Val Asn Ala Ala Asp Ala Ala Ala Leu Glu His Asp Lys Ala Tyr Asp
65 70 75 80
Gln Gln Leu Lys Ala Gly Asp Asn Pro Tyr Pro Arg Tyr Asn His Ala
85 90 95
Asp Ala Glu Phe Gln Glu Arg Leu Gln Glu Asp Thr Pro Phe Gly Gly
100 105 110
Asn Leu Gly Arg Ala Val Phe Gln Ala Lys Lys Arg Val Leu Glu Pro
115 120 125
Leu Gly Leu Val Glu Glu Ala Ala Lys Thr Ala Pro Gly Lys Lys Arg
130 135 140
Pro Val Glu Pro Ser Pro Gln Arg Ser Pro Asp Ser Ser Thr Gly Ile
145 150 155 160
Gly Lys Lys Gly Gln Gln Pro Ala Lys Lys Arg Leu Asn Phe Gly Gln
165 170 175
Thr Gly Asp Ser Glu Ser Val Pro Asp Pro Gln Pro Ile Gly Glu Pro
180 185 190
Pro Ala Gly Pro Ser Gly Leu Gly Ser Gly Thr Met Ala Ala Gly Gly
195 200 205
Gly Ala Pro Met Ala Asp Asn Asn Glu Gly Ala Asp Gly Val Gly Asn
210 215 220
Ala Ser Gly Asn Trp His Cys Asp Ser Thr Trp Leu Gly Asp Arg Val
225 230 235 240
Ile Thr Thr Ser Thr Arg Thr Trp Ala Leu Pro Thr Tyr Asn Asn His
245 250 255
Leu Tyr Lys Gln Ile Ser Ser Ala Ser Thr Gly Ala Ser Asn Asp Asn
260 265 270
His Tyr Phe Gly Tyr Ser Thr Pro Trp Gly Tyr Phe Asp Phe Asn Arg
275 280 285
Phe His Cys His Phe Ser Pro Arg Asp Trp Gln Arg Leu Ile Asn Asn
290 295 300
Asn Trp Gly Phe Arg Pro Lys Arg Leu Asn Phe Lys Leu Phe Asn Ile
305 310 315 320
Gln Val Lys Glu Val Thr Thr Asn Asp Gly Val Thr Thr Ile Ala Asn
325 330 335
Asn Leu Thr Ser Thr Val Gln Val Phe Ser Asp Ser Glu Tyr Gln Leu
340 345 350
Pro Tyr Val Leu Gly Ser Ala His Gln Gly Cys Leu Pro Pro Phe Pro
355 360 365
Ala Asp Val Phe Met Ile Pro Gln Tyr Gly Tyr Leu Thr Leu Asn Asn
370 375 380
Gly Ser Gln Ala Val Gly Arg Ser Ser Phe Tyr Cys Leu Glu Tyr Phe
385 390 395 400
Pro Ser Gln Met Leu Arg Thr Gly Asn Asn Phe Thr Phe Ser Tyr Thr
405 410 415
Phe Glu Glu Val Pro Leu His Ser Ser Tyr Ala His Ser Gln Ser Leu
420 425 430
Asp Arg Leu Met Asn Pro Leu Ile Val Gln Tyr Leu Tyr Tyr Leu Asn
435 440 445
Arg Thr Gln Asn Gln Ser Gly Ser Ala Gln Asn Lys Asp Leu Leu Phe
450 455 460
Ser Arg Gly Ser Pro Ala Gly Met Ser Val Gln Pro Lys Asn Trp Leu
465 470 475 480
Pro Gly Pro Cys Tyr Arg Gln Gln Arg Val Ser Lys Thr Lys Thr Asp
485 490 495
Asn Asn Asn Ser Asn Phe Thr Trp Thr Gly Ala Ser Lys Tyr Asn Leu
500 505 510
Asn Gly Arg Glu Ser Ile Ile Asn Pro Gly Thr Ala Met Ala Ser His
515 520 525
Lys Asp Asp Glu Asp Lys Phe Phe Pro Met Ser Gly Val Met Ile Phe
530 535 540
Gly Lys Glu Ser Ala Gly Ala Ser Asn Thr Ala Leu Asp Asn Val Met
545 550 555 560
Ile Thr Asp Glu Glu Glu Ile Lys Ala Thr Asn Pro Val Ala Thr Glu
565 570 575
Arg Phe Gly Thr Val Ala Val Asn Phe Gln Ser Ser Ser Thr Asp Pro
580 585 590
Ala Thr Gly Asp Val His Ala Met Gly Ala Leu Pro Gly Met Val Trp
595 600 605
Gln Asp Arg Asp Val Tyr Leu Gln Gly Pro Ile Trp Ala Lys Ile Pro
610 615 620
His Thr Asp Gly His Phe His Pro Ser Pro Leu Met Gly Gly Phe Gly
625 630 635 640
Leu Lys Asn Pro Pro Pro Gln Ile Leu Ile Lys Asn Thr Pro Val Pro
645 650 655
Ala Asn Pro Pro Ala Glu Phe Ser Ala Thr Lys Phe Ala Ser Phe Ile
660 665 670
Thr Gln Tyr Ser Thr Gly Gln Val Ser Val Glu Ile Glu Trp Glu Leu
675 680 685
Gln Lys Glu Asn Ser Lys Arg Trp Asn Pro Glu Val Gln Tyr Thr Ser
690 695 700
Asn Tyr Ala Lys Ser Ala Ser Val Asp Phe Thr Val Asp Asn Asn Gly
705 710 715 720
Leu Tyr Thr Glu Pro Arg Pro Ile Gly Thr Arg Tyr Leu Thr Arg Pro
725 730 735
Leu
<210>161
<211>738
<212>PRT
<213>vp1,克隆hu.38
<400>161
Met Ala Ala Asp Gly Tyr Leu Pro Asp Trp Leu Glu Asp Asn Leu Ser
1 5 10 15
Glu Gly Ile Arg Glu Trp Trp Asp Leu Lys Pro Gly Ala Pro Lys Pro
20 25 30
Lys Ala Asn Gln Gln Lys Gln Asp Asp Gly Arg Gly Leu Val Leu Pro
35 40 45
Gly Tyr Lys Tyr Leu Gly Pro Phe Asn Gly Leu Asp Lys Gly Glu Pro
50 55 60
Val Asn Ala Ala Asp Ala Ala Ala Leu Glu His Asp Lys Ala Tyr Asp
65 70 75 80
Gln Gln Leu Lys Ala Gly Asp Asn Pro Tyr Leu Arg Tyr Asn His Ala
85 90 95
Asp Ala Glu Phe Gln Glu Arg Leu Gln Glu Asp Thr Ser Phe Gly Gly
100 105 110
Asn Leu Gly Arg Ala Val Phe Gln Ala Lys Lys Arg Val Leu Glu Pro
115 120 125
Leu Gly Leu Val Glu Glu Ala Ala Lys Thr Ala Pro Gly Lys Lys Arg
130 135 140
Pro Val Glu Pro Ser Pro Gln Arg Ser Pro Asp Ser Ser Thr Gly Ile
145 150 155 160
Gly Lys Lys Gly Gln Gln Pro Ala Lys Lys Arg Leu Asn Phe Gly Gln
165 170 175
Thr Gly Asp Ser Glu Ser Val Pro Asp Pro Gln Pro Ile Gly Glu Pro
180 185 190
Pro Ala Gly Pro Ser Gly Leu Gly Ser Gly Thr Met Ala Ala Gly Gly
195 200 205
Gly Ala Pro Met Ala Asp Asn Asn Glu Gly Ala Asp Gly Val Gly Ser
210 215 220
Ser Ser Gly Asn Trp His Cys Asp Ser Thr Trp Leu Gly Asp Arg Val
225 230 235 240
Ile Thr Thr Ser Thr Arg Thr Trp Ala Leu Pro Thr Tyr Asn Asn His
245 250 255
Leu Tyr Lys Gln Ile Ser Asn Gly Thr Ser Gly Gly Ser Thr Asn Asp
260 265 270
Asn Thr Tyr Phe Gly Tyr Ser Thr Pro Trp Gly Tyr Phe Asp Phe Asn
275 280 285
Arg Phe His Cys His Phe Ser Pro Arg Asp Trp Gln Arg Leu Ile Asn
290 295 300
Asn Asn Trp Gly Phe Arg Pro Lys Arg Leu Ser Phe Lys Leu Phe Asn
305 310 315 320
Ile Gln Val Lys Glu Val Thr Gln Asn Glu Gly Thr Lys Thr Ile Ala
325 330 335
Asn Asn Leu Thr Ser Thr Ile Gln Val Phe Thr Asp Ser Glu Tyr Gln
340 345 350
Leu Pro Tyr Val Leu Gly Ser Ala His Gln Gly Cys Leu Pro Pro Phe
355 360 365
Pro Ala Asp Val Phe Met Ile Pro Gln Tyr Gly Tyr Leu Thr Leu Asn
370 375 380
Asn Gly Ser Gln Ala Val Gly Arg Ser Ser Phe Tyr Cys Leu Glu Tyr
385 390 395 400
Phe Pro Ser Gln Met Leu Arg Thr Gly Asn Asn Phe Glu Phe Ser Tyr
405 410 415
Thr Phe Glu Asp Val Pro Phe His Ser Ser Tyr Ala His Ser Gln Ser
420 425 430
Leu Asp Arg Leu Met Asn Pro Leu Ile Asp Gln Tyr Leu Tyr Tyr Leu
435 440 445
Ser Arg Thr Gln Ser Thr Gly Gly Thr Gln Gly Thr Gln Gln Leu Leu
450 455 460
Phe Ser Gln Ala Gly Pro Ala Asn Met Ser Ala Gln Ala Lys Asn Trp
465 470 475 480
Leu Pro Gly Pro Cys Tyr Arg Gln Gln Arg Val Ser Thr Thr Leu Ser
485 490 495
Gln Asn Asn Asn Ser Asn Phe Ala Trp Thr Gly Ala Thr Lys Tyr His
500 505 510
Leu Asn Gly Arg Asp Ser Leu Val Asn Pro Gly Val Ala Met Ala Thr
515 520 525
His Lys Asp Asp Glu Glu Arg Phe Phe Pro Ser Ser Gly Val Leu Met
530 535 540
Phe Gly Lys Gln Gly Ala Gly Arg Asp Asn Val Asp Tyr Ser Ser Val
545 550 555 560
Met Leu Thr Ser Glu Glu Glu Ile Lys Thr Thr Asn Pro Val Ala Thr
565 570 575
Glu Gln Tyr Gly Val Val Ala Asp Asn Leu Gln Gln Thr Asn Thr Gly
580 585 590
Pro Ile Val Gly Asn Val Asn Ser Gln Gly Ala Leu Pro Gly Met Val
595 600 605
Trp Gln Asn Arg Asp Val Tyr Leu Gln Gly Pro Ile Trp Ala Lys Ile
610 615 620
Pro His Thr Asp Gly Asn Cys His Pro Ser Pro Leu Met Gly Gly Phe
625 630 635 640
Gly Leu Lys His Pro Pro Pro Gln Ile Leu Ile Lys Asn Thr Pro Val
645 650 655
Pro Ala Asp Pro Pro Thr Thr Phe Ser Gln Ala Lys Leu Ala Ser Phe
660 665 670
Ile Thr Gln Tyr Ser Thr Gly Gln Val Ser Val Glu Ile Glu Trp Glu
675 680 685
Leu Gln Lys Glu Asn Ser Lys Arg Trp Asn Pro Glu Ile Gln Tyr Thr
690 695 700
Ser Asn Tyr Tyr Lys Ser Thr Asn Val Asp Phe Ala Val Asn Thr Glu
705 710 715 720
Gly Thr Tyr Ser Glu Pro Arg Pro Ile Gly Thr Arg Tyr Leu Thr Arg
725 730 735
Asn Leu
<210>162
<211>736
<212>PRT
<213>vp1,克隆rh.71
<400>162
Met Ala Ala Asp Gly Tyr Leu Pro Asp Trp Leu Glu Asp Asn Leu Ser
1 5 10 15
Glu Gly Ile Arg Glu Trp Trp Asp Leu Lys Pro Gly Ala Pro Lys Pro
20 25 30
Lys Ala Asn Gln Gln Lys Gln Asp Asp Gly Arg Gly Leu Val Leu Pro
35 40 45
Gly Tyr Lys Tyr Leu Gly Pro Phe Asn Gly Leu Asp Lys Gly Glu Pro
50 55 60
Val Asn Ala Ala Asp Ala Ala Ala Leu Glu His Asp Lys Ala Tyr Asp
65 70 75 80
Gln Gln Leu Lys Ala Gly Asp Asn Pro Tyr Leu Arg Tyr Asn His Ala
85 90 95
Asp Ala Glu Phe Gln Glu Arg Leu Gln Glu Asp Thr Ser Phe Gly Gly
100 105 110
Asn Leu Gly Arg Ala Val Phe Gln Ala Lys Lys Arg Val Leu Glu Pro
115 120 125
Leu Gly Leu Val Glu Glu Gly Ala Lys Thr Ala Pro Gly Lys Lys Arg
130 135 140
Pro Val Glu Gln Ser Pro Gln Glu Pro Asp Ser Ser Ser Gly Ile Gly
145 150 155 160
Lys Thr Gly Gln Gln Pro Ala Lys Lys Arg Leu Asn Phe Gly Gln Thr
165 170 175
Gly Asp Ser Glu Ser Val Pro Asp Pro Gln Pro Leu Gly Glu Pro Pro
180 185 190
Ala Thr Pro Ala Ala Val Gly Pro Thr Thr Met Ala Ser Gly Gly Gly
195 200 205
Ala Pro Met Ala Asp Asn Asn Glu Gly Ala Asp Gly Val Gly Asn Ala
210 215 220
Ser Gly Asn Trp His Cys Asp Ser Thr Trp Leu Gly Asp Arg Val Ile
225 230 235 240
Thr Thr Ser Thr Arg Thr Trp Ala Leu Pro Thr Tyr Asn Asn His Leu
245 250 255
Tyr Lys Gln Ile Ser Ser Ala Ser Thr Gly Ala Ser Asn Asp Asn His
260 265 270
Tyr Phe Gly Tyr Ser Thr Pro Trp Gly Tyr Phe Asp Phe Asn Arg Phe
275 280 285
His Cys His Phe Ser Pro Arg Asp Trp Gln Arg Leu Ile Asn Asn Asn
290 295 300
Trp Gly Phe Arg Pro Lys Arg Leu Asn Phe Lys Leu Phe Asn Ile Gln
305 310 315 320
Val Lys Glu Val Thr Thr Asn Asp Gly Val Thr Thr Ile Ala Asn Asn
325 330 335
Leu Thr Ser Thr Val Gln Val Phe Ser Asp Ser Glu Tyr Gln Leu Pro
340 345 350
Tyr Val Leu Gly Ser Ala His Gln Gly Cys Leu Pro Pro Phe Pro Ala
355 360 365
Asp Val Phe Met Ile Pro Gln Tyr Gly Tyr Leu Thr Leu Asn Asn Gly
370 375 380
Ser Gln Ala Val Gly Arg Ser Ser Phe Tyr Cys Leu Glu Tyr Phe Pro
385 390 395 400
Ser Gln Met Leu Arg Thr Gly Asn Asn Phe Thr Phe Ser Tyr Thr Phe
405 410 415
Glu Glu Val Pro Phe His Ser Ser Tyr Ala Hi s Ser Gln Ser Leu Asp
420 425 430
Arg Leu Met Asn Pro Leu Ile Asp Gln Tyr Leu Tyr Tyr Leu Asn Arg
435 440 445
Thr Gln Asn Gln Ser Gly Ser Ala Gln Asn Lys Asp Leu Leu Phe Ser
450 455 460
Arg Gly Ser Pro Ala Gly Met Ser Val Gln Pro Lys Asn Trp Leu Pro
465 470 475 480
Gly Pro Cys Tyr Arg Gln Gln Arg Val Ser Lys Thr Lys Thr Asp Asn
485 490 495
Asn Asn Ser Asn Phe Thr Trp Thr Gly Ala Ser Lys Tyr Asn Leu Asn
500 505 510
Gly Arg Glu Ser Ile Ile Asn Pro Gly Thr Ala Met Ala Ser His Lys
515 520 525
Asp Asp Glu Asp Lys Phe Phe Pro Met Ser Gly Val Met Ile Phe Gly
530 535 540
Lys Glu Ser Ala Gly Ala Ser Asn Thr Ala Leu Asp Asn Val Met Ile
545 550 555 560
Thr Asp Glu Glu Glu Ile Lys Ala Thr Asn Pro Val Ala Thr Glu Arg
565 570 575
Phe Gly Thr Val Ala Val Asn Phe Gln Ser Ser Ser Thr Asp Pro Ala
580 585 590
Thr Gly Asp Val His Ala Met Gly Ala Leu Pro Gly Met Val Trp Gln
595 600 605
Asp Arg Asp Val Tyr Leu Gln Gly Pro Ile Trp Ala Lys Ile Pro His
610 615 620
Thr Asp Gly His Phe His Pro Ser Pro Leu Met Gly Gly Phe Gly Leu
625 630 635 640
Lys Asn Pro Pro Pro Gln Ile Leu Ile Lys Asn Thr Pro Val Pro Ala
645 650 655
Asn Pro Pro Ala Glu Phe Ser Ala Thr Lys Phe Ala Ser Phe Ile Thr
660 665 670
Gln Tyr Ser Thr Gly Gln Val Ser Val Glu Ile Glu Trp Glu Leu Gln
675 680 685
Lys Glu Asn Ser Lys Arg Trp Asn Pro Glu Val Gln Tyr Thr Ser Asn
690 695 700
Tyr Ala Lys Ser Ala Asn Val Asp Phe Thr Val Asp Asn Asn Gly Leu
705 710 715 720
Tyr Thr Glu Pro Arg Pro Ile Gly Thr Arg Tyr Leu Thr Arg Pro Leu
725 730 735
<210>163
<211>736
<212>PRT
<213>vp1,克隆rh.43
<400>163
Met Ala Ala Asp Gly Tyr Leu Pro Asp Trp Leu Glu Asp Asn Leu Ser
1 5 10 15
Glu Gly Ile Arg Glu Trp Trp Asp Leu Lys Pro Gly Ala Pro Lys Pro
20 25 30
Lys Ala Asn Gln Gln Lys Gln Asp Asp Gly Arg Gly Leu Val Leu Pro
35 40 45
Gly Tyr Lys Tyr Leu Gly Pro Phe Asn Gly Leu Asp Lys Gly Glu Pro
50 55 60
Val Asn Ala Ala Asp Ala Ala Ala Leu Glu His Asp Lys Ala Tyr Asp
65 70 75 80
Gln Gln Leu Glu Ala Gly Asp Asn Pro Tyr Leu Arg Tyr Asn His Ala
85 90 95
Asp Ala Glu Phe Gln Glu Arg Leu Gln Glu Asp Thr Ser Phe Gly Gly
100 105 110
Asn Leu Gly Arg Ala Val Phe Gln Ala Lys Lys Arg Val Leu Glu Pro
115 120 125
Leu Gly Leu Val Glu Glu Gly Ala Lys Thr Ala Pro Gly Lys Lys Arg
130 135 140
Pro Val Glu Gln Ser Pro Gln Glu Pro Asp Ser Ser Ser Gly Ile Gly
145 150 155 160
Lys Lys Gly Gln Gln Pro Ala Arg Lys Arg Leu Asn Phe Gly Gln Thr
165 170 175
Gly Asp Ser Glu Ser Val Pro Asp Pro Gln Pro Leu Gly Glu Pro Pro
180 185 190
Ala Ala Pro Ser Gly Val Gly Pro Asn Thr Met Ala Ala Gly Gly Gly
195 200 205
Ala Pro Met Ala Asp Asn Asn Glu Gly Ala Asp Gly Val Gly Ser Ser
210 215 220
Ser Gly Asn Trp His Cys Asp Ser Thr Trp Leu Gly Asp Arg Val Ile
225 230 235 240
Thr Thr Ser Thr Arg Thr Trp Ala Leu Pro Thr Tyr Asn Asn His Leu
245 250 255
Tyr Lys Gln Ile Ser Asn Gly Thr Ser Gly Gly Ala Thr Asn Asp Asn
260 265 270
Thr Tyr Phe Gly Tyr Ser Thr Pro Trp Gly Tyr Phe Asp Phe Asn Arg
275 280 285
Phe His Cys His Phe Ser Pro Arg Asp Trp Gln Arg Leu Ile Asn Asn
290 295 300
Asn Trp Gly Phe Arg Pro Lys Arg Leu Ser Phe Lys Leu Phe Asn Ile
305 310 315 320
Gln Val Lys Glu Val Thr Gln Asn Glu Gly Thr Lys Thr Ile Ala Asn
325 330 335
Asn Leu Thr Ser Thr Ile Gln Val Phe Thr Asp Ser Glu Tyr Gln Leu
340 345 350
Pro Tyr Val Leu Gly Ser Ala His Gln Gly Cys Leu Pro Pro Phe Pro
355 360 365
Ala Asp Val Phe Met Ile Pro Gln Tyr Gly Tyr Leu Thr Leu Asn Asn
370 375 380
Gly Ser Gln Ala Val Gly Arg Ser Ser Phe Tyr Cys Leu Glu Tyr Phe
385 390 395 400
Pro Ser Gln Met Leu Arg Thr Gly Asn Asn Phe Gln Phe Thr Tyr Thr
405 410 415
Phe Glu Asp Val Pro Phe His Ser Ser Tyr Ala His Ser Gln Ser Leu
420 425 430
Asp Arg Leu Met Asn Pro Leu Ile Asp Gln Tyr Leu Tyr Tyr Leu Ser
435 440 445
Arg Thr Gln Thr Thr Gly Gly Thr Ala Asn Thr Gln Thr Leu Gly Phe
450 455 460
Ser Gln Gly Gly Pro Asn Thr Met Ala Asn Gln Ala Lys Asn Trp Leu
465 470 475 480
Pro Gly Pro Cys Tyr Arg Gln Gln Arg Val Ser Thr Thr Thr Gly Gln
485 490 495
Asn Asn Asn Ser Asn Phe Ala Trp Thr Ala Gly Thr Lys Tyr His Leu
500 505 510
Asn Gly Arg Asn Ser Leu Ala Asn Pro Gly Ile Ala Met Ala Thr His
515 520 525
Lys Asp Asp Glu Glu Arg Phe Phe Pro Val Thr Gly Ser Cys Phe Trp
530 535 540
Gln Gln Asn Ala Ala Arg Asp Asn Ala Asp Tyr Ser Asp Val Met Leu
545 550 555 560
Thr Ser Glu Glu Glu Ile Lys Thr Thr Asn Pro Val Ala Thr Glu Glu
565 570 575
Tyr Gly Ile Val Ala Asp Asn Leu Gln Gln Gln Asn Thr Ala Pro Gln
580 585 590
Ile Gly Thr Val Asn Ser Gln Gly Ala Leu Pro Gly Met Val Trp Gln
595 600 605
Asn Arg Asp Val Tyr Leu Gln Gly Pro Ile Trp Ala Lys Ile Pro His
610 615 620
Thr Asp Gly Asn Phe His Pro Ser Pro Leu Met Gly Gly Phe Gly Leu
625 630 635 640
Lys His Pro Pro Pro Gln Ile Leu Ile Lys Asn Thr Pro Val Pro Ala
645 650 655
Asp Pro Pro Thr Thr Phe Asn Gln Ser Lys Leu Asn Ser Phe Ile Thr
660 665 670
Gln Tyr Ser Thr Gly Gln Val Ser Val Glu Ile Glu Trp Glu Leu Gln
675 680 685
Lys Glu Asn Ser Lys Arg Trp Asn Pro Glu Ile Gln Tyr Thr Ser Asn
690 695 700
Tyr Tyr Lys Ser Thr Ser Val Asp Phe Ala Val Asn Thr Glu Gly Val
705 710 715 720
Tyr Ser Glu Pro Arg Pro Ile Gly Thr Arg Tyr Leu Thr Arg Asn Leu
725 730 735
<210>164
<211>735
<212>PRT
<213>vp1,克隆hu.35
<400>164
Met Ala Ala Asp Gly Tyr Leu Pro Asp Trp Leu Glu Asp Thr Leu Ser
1 5 10 15
Glu Gly Ile Arg Gln Arg Trp Lys Leu Lys Pro Gly Pro Pro Pro Pro
20 25 30
Glu Pro Ala Glu Arg His Lys Asp Asp Ser Arg Gly Leu Val Leu Pro
35 40 45
Gly Tyr Lys Tyr Leu Gly Pro Phe Asn Gly Leu Asp Lys Gly Glu Pro
50 55 60
Val Asn Glu Ala Asp Ala Ala Ala Leu Glu His Asp Lys Ala Tyr Asp
65 70 75 80
Arg Gln Leu Asp Ser Gly Asp Asn Pro Tyr Leu Lys Tyr Asn His Ala
85 90 95
Asp Ala Glu Phe Gln Glu Arg Leu Lys Glu Asp Thr Ser Phe Gly Gly
100 105 110
Asn Leu Gly Arg Ala Val Phe Gln Ala Lys Lys Arg Val Leu Glu Pro
115 120 125
Leu Gly Leu Val Glu Glu Pro Val Lys Thr Ala Pro Gly Lys Lys Arg
130 135 140
Pro Val Glu His Ser Pro Val Glu Pro Asp Ser Ser Ser Gly Thr Gly
145 150 155 160
Lys Ala Gly Gln Gln Pro Ala Arg Lys Arg Leu Asn Phe Gly Gln Thr
165 170 175
Gly Asp Ala Asp Ser Val Pro Asp Pro Gln Pro Leu Gly Gln Pro Pro
180 185 190
Ala Ala Pro Ser Gly Leu Gly Thr Asn Thr Met Ala Thr Gly Ser Gly
195 200 205
Ala Pro Met Ala Asp Asn Asn Glu Gly Ala Asp Gly Val Gly Asn Ser
210 215 220
Ser Gly Asn Trp His Cys Asp Ser Thr Trp Met Gly Asp Arg Val Ile
225 230 235 240
Thr Thr Ser Thr Arg Thr Trp Ala Leu Pro Thr Tyr Asn Asn His Leu
245 250 255
Tyr Lys Gln Ile Ser Ser Gin Ser Gly Ala Ser Asn Asp Asn His Tyr
260 265 270
Phe Gly Tyr Ser Thr Pro Trp Gly Tyr Phe Asp Phe Asn Arg Phe His
275 280 285
Cys His Phe Ser Pro Arg Asp Trp Gln Arg Leu Ile Asn Asn Asn Trp
290 295 300
Gly Phe Arg Pro Lys Arg Leu Asn Phe Lys Leu Phe Asn Ile Gln Val
305 310 315 320
Lys Glu Val Thr Gln Asn Asp Gly Thr Thr Thr Ile Ala Asn Asn Leu
325 330 335
Thr Ser Thr Val Gln Val Phe Thr Asp Ser Glu Tyr Gln Leu Pro Tyr
340 345 350
Val Leu Gly Ser Ala His Gln Gly Cys Leu Pro Pro Phe Pro Ala Asp
355 360 365
Val Phe Met Val Pro Gln Tyr Gly Tyr Leu Thr Leu Asn Asn Gly Ser
370 375 380
Gln Ala Val Gly Arg Ser Ser Phe Tyr Cys Leu Glu Tyr Phe Pro Ser
385 390 395 400
Gln Met Leu Arg Thr Gly Asn Asn Phe Thr Phe Ser Tyr Thr Phe Glu
405 410 415
Asp Val Pro Phe His Ser Ser Tyr Ala His Ser Gln Ser Leu Gly Arg
420 425 430
Leu Met Asn Pro Leu Ile Asp Gln Tyr Leu Tyr Tyr Leu Ser Arg Thr
435 440 445
Asn Thr Pro Ser Gly Thr Thr Thr Gln Ser Arg Leu Gln Phe Ser Gln
450 455 460
Ala Gly Ala Ser Asp Ile Arg Asp Gln Ser Arg Asn Trp Leu Pro Gly
465 470 475 480
Pro Cys Tyr Arg Gln Gln Arg Val Ser Lys Thr Ser Ala Asp Asn Asn
485 490 495
Asn Ser Glu Tyr Ser Trp Thr Gly Ala Thr Lys Tyr His Leu Asn Gly
500 505 510
Arg Asp Ser Leu Val Asn Pro Gly Pro Ala Met Ala Ser His Lys Asp
515 520 525
Asp Glu Glu Lys Phe Phe Pro Gln Ser Gly Val Leu Ile Phe Gly Lys
530 535 540
Gln Gly Ser Glu Lys Thr Asn Val Asp Ile Glu Lys Val Met Ile Thr
545 550 555 560
Asp Glu Glu Glu Ile Arg Thr Thr Asn Pro Val Ala Thr Glu Gln Tyr
565 570 575
Gly Ser Val Ser Thr Asn Leu Gln Arg Gly Asn Arg Gln Ala Ala Thr
580 585 590
Ala Asp Val Asn Thr Gln Gly Val Leu Pro Gly Met Val Trp Gln Asp
595 600 605
Arg Asp Val Tyr Leu Gln Gly Pro Ile Trp Ala Lys Ile Pro His Thr
610 615 620
Asp Gly His Phe His Pro Ser Pro Leu Met Gly Gly Phe Gly Leu Lys
625 630 635 640
His Pro Pro Pro Gln Ile Leu Ile Lys Asn Thr Pro Val Pro Ala Asn
645 650 655
Pro Ser Thr Thr Phe Ser Ala Ala Lys Phe Ala Ser Phe Ile Thr Gln
660 665 670
Tyr Ser Thr Gly Gln Val Ser Val Glu Ile Glu Trp Glu Leu Gln Lys
675 680 685
Glu Asn Ser Lys Arg Trp Asn Pro Glu Ile Gln Tyr Thr Ser Asn Tyr
690 695 700
Asn Lys Ser Val Asn Val Asp Phe Thr Val Asp Thr Asn Gly Val Tyr
705 710 715 720
Ser Glu Pro Arg Pro Ile Gly Thr Arg Tyr Leu Thr Arg Asn Leu
725 730 735
<210>165
<211>3161
<212>DNA
<213>新AAV血清型,克隆hu136.1
<400>165
gattgaattt agcggccgcg aattcgccct tgctgcgtca actggaccaa tgagaacttt 60
ccattcaatg attgcgtcga caagatggtg atctggtggg aggagggaaa gatgaccgcc 120
aaggtcatgg agtcggccaa agccattctc ggaggaagca aggtgcgcgt ggaccagaaa 180
tgtaagtcct cggcccagat agacccgact cccgtgattg tcacctccaa caccaacatg 240
tgcgccgtga ttgacgggaa ctcaacgacc ttcgagcacc agcagccgtt gcaagaccgg 300
atgttcaaat ttgaactcac ccgccgtctg gatcatgact ttgggaaggt caccaagcag 360
gaagtcaaag actttttccg gtgggcaaag gatcacgtgg ttgaggtgga gcatgaattc 420
tacgtcaaaa agggtggagc caagaaaaga cccgccccca gtgacgcaga tataagtgag 480
cccaaacggg cgcgcgggtc agttgcgcag ccatcgacgt cagacgcgga agcttcgatc 540
aactacgcgg acaggtacca aaacaaatgt tctcgtcacg tgggcatgaa tctgatgctg 600
tttccctgca gacaatgcga gagaatgaat caaaattcaa atatctgctt cactcacgga 660
cagaaggact gtttagagtg ctttcccgtg tcagaatctc aacccgtttc tgtcgtcaaa 720
aaggcgtatc agaaactttg ctacattcat catatcatgg gaaaggtgcc agacgcttgc 780
actgcctgcg atctggtcaa tgtggatttg gatgactgca tctctgaaca ataaatgatt 840
taaatcaggt atggctgccg atggttatct tccagattgg ctcaaggaca ctctctctga 900
aggaataaga cagtggtgga agctcaaacc tggcccacca ccaccaaagc ccgcagagcg 960
gcataaggac gacagcgggg gtcttgtgct tcctgggtac aagtacctcg gacccttcaa 1020
cggactcgac aagggagagc cggtcaacga ggcagacgcc gcggccctcg agtacgacaa 1080
ggcctacgac cggcagctcg acagcggaga caacccgtac ctcaagtaca accacgccga 1140
cgcggagttt caggagcgcc ttaaagaaga tacgtctttt gggggcaacc tcggacgagc 1200
agtcttccag gcgaaaaaga gggttcttga acctctgggc ctggttgagg aacctgttaa 1260
gacggctccg ggaaaaaaga ggccggtaga gcactctcct gtggagccag actcctcctc 1320
gggaacagga aaagcgggcc agcagcctgc gagaaagaga ttgaattttg gtcagactgg 1380
agacgcagac tccgtacctg acccccagcc tctcggacag ccaccagcag ccccctctgg 1440
tctgggaact aatacgatgg ctacaggcag tggcgcacca atggcagaca ataacgaggg 1500
cgccgacgga gtgggtaatt cctcgggaag ttggcattgc gattccacat ggatgggcga 1560
cagagtcatc accaccagca cccgaacctg ggctctgccc acctacaaca accatctgta 1620
caagcagatc tccagccaat caggagccag caacgacaac cactactttg gctacagcac 1680
cccttggggg tattttgact tcaacagatt ccactgccac ttttcaccac gtgactggca 1740
aagactcatc aacaacaact ggggattccg gcccaagaga ctcaacttca agctctttaa 1800
cattcaagtc aaggaggtca cgcagaatga cggtacgacg acgattgcca ataaccttac 1860
cagcacggtt caggtgttta ctgactcgga gtaccagctc ccgtacgtcc ccggctcggc 1920
gcatcaagga tgcctcccgc cgttcccagc agacgtcttc atggtgccac agtatggata 1980
cctcaccctg aacaacggga gtcaggcagt aggacgctct tcattttact gcctggagta 2040
ctttccttct cagatgcttc gtaccggaaa caactttacc ttcagctaca cctttgagga 2100
tgttcctttc cacagcagct acgctcacag ccagagtttg gaccgtctca tgaatcctct 2160
catcgaccag tacctgtatt acttgagcag aacaaacact ccaagcggaa ccaccacgca 2220
gtccaggctt cagttttctc aggccggagc aagtgacatt cgggaccagt ctaggaactg 2280
gcttcctgga ccctgttacc gccagcagcg agtatcaaag acagctgcgg acaacaacaa 2340
cagtgaatac tcgtggactg gagctaccaa gtaccacctc aatggcagag actctctggt 2400
gaatccgggc ccggccatgg ccagccacaa ggacgatgaa gaaaagtttt ttcctcaaag 2460
cggggttctc atctttggga agcaaggctc agagaaaaca aatgtggaca ttgaaaaggt 2520
catgattaca gacgaagagg aaatcagaac caccaatccc gtggccacgg agcagtatgg 2580
ttctgtatct accaacctcc agagcggcaa cacacaagca gctactgcag atgtcaacac 2640
acaaggcgtt cttccaggca tggtctggca ggacagagac gtgcacctgc aggggcctat 2700
ctgggcaaag attccacaca cggacggaca ttttcacccc tctcccctca tgggcggatt 2760
tggacttaaa caccctcctc cacagattct catcaagaac accccggtac ctgcaaatcc 2820
ttcgaccacc ttcagtgcgg caaagtttgc ttccttcatc acacagtatt ccacagggca 2880
ggtcagcgtg gagatcgagt gggagctgca gaaggagaac agcaaacgct ggaaccccga 2940
gatccagtac acttccaact acaacaaatc tgttaatgtg gactttactg tggacactaa 3000
tggtgtgtat tcagagcctc gccccattgg caccagatac ctgactcgta atctgtaatt 3060
gcttgttaat caataaaccg tttaattcgt ttcagttgaa ctttggtctc tgcgaagggc 3120
gaattcgttt aaaccctgca ggactagtcc ctttagtgag g 3161
<210>166
<211>3162
<212>DNA
<213>新AAV血清型,克隆hu140.1
<400>166
gaattgaatt tagcggccgc gaattcgccc ttcgcagaga ccaaagttca actgaaacga 60
attaaacggt ttattgatta acaagcaatt acagattacg agtcaggtat ctggtgccaa 120
tggggcgagg ctctgaatac acgccattag tgtccacagt aaagtccaca ttaacagact 180
tgttgtagtt ggaagtgtac tgaatttcgg gattccagcg tttgctgttt tccttctgca 240
gctcccactc gatctccacg ctgacctgtc ccgtggagta ctgtgtgatg aaggaagcaa 300
actttgccgc actgaaggtg gtcgaaggat tcgcaggtac cggggtgttc ttgatgagaa 360
tctgtggagg agggtgttta agtccgaatc cacccatgag gggagagggg tgaaaatgtc 420
cgtccgtgtg tggaatcttt gcccagatgg gcccctgaag gtacacatct ctgtcctgcc 480
agaccatgcc tggaagaacg ccttgtgtgt tgacatctgc ggtagctgct tgtctgttgc 540
ctctctggag gttggtagat acagaaccgt actgctccgt agccacggga ttggttgtcc 600
tgatttcctc ttcgtctgta atcatgacct tttcaatgtc cacatttgtt ttctctgagc 660
cttgcttccc aaagatgaga accccgctct gaggaaaaaa cttttcttca ttgtccttgt 720
ggcttgccat ggccgggccc ggattcacca gagagtctct gccattgagg tggtacttgg 780
tagctccagt ccacgagtat tcactgttgt tgttatccgc agatgtcttt gatactcgct 840
gctggcggta acagggtcca ggaagccagt tcctagactg gtcccgaatg tcactcgctc 900
cggcctgaga aaactgaagc cttgactgcg tggtggttcc acttggagtg tttgttgtgc 960
tcaagtaata caggtactgg tcgatgagag gattcatgag acggtccaga ctctggctgt 1020
gagcgtagcc gctgtggaaa ggaacgtcct caaaagtgta gctgaaggta aagttgtttc 1080
cggtacgcag catctgagaa ggaaagtact ccaggcagta aaatgaagag cgtcctactg 1140
cctgactccc gttgttcagg gtgaggtatc catactgtgg caccatgaag acgtctgctg 1200
ggaacggcgg gaggcatcct tgatgcgccg agccgaggac gtacgggagc tggtactccg 1260
agtcagtaaa cacctgaacc gtgctggtaa ggttattggc aatcgtcgtc gtaccgtcat 1320
tctgcgtgac ctctttgact tgaatgttaa agagcttgaa gttgagtctc ttgggtcgga 1380
atccccagtt gttgttgatg agtctttgcc agtcacgtgg tgaaaagtgg cagtggaatc 1440
tgttgaagtc aaaatacccc caaggggtgc tgtagccaaa gtagtgattg tcgttcgagg 1500
ctcctgattg gctggaaatt tgtttgtaga ggtggttgtt gtaggtgggc agggcccagg 1560
ttcgggtgct ggtggtgatg actctgtcgc ccatccatgt ggaatcgcaa tgccaatttc 1620
ccgaggaatt acccactccg tcggcgccct cgttattgtc tgccattggt gcgccactgc 1680
ctgtagccat cgtattagtt cccagaccag agggggctgc tggtggctgt ccgagaggct 1740
gggggtcagg tactgagtct gcgtctccag tctgaccaaa attcaatctt tttcttgcag 1800
gctgctggcc cgcctttccg gttcccgagg aggagtctgg ctccacagga gagtgctcta 1860
ccggcctctt ttttcctgga gccgtcttga caggttcccc aaccaggccc agaggttcaa 1920
gaaccctctt tttcgcctgg aagactgctc gtccgaggtt gcccccaaaa gacgtatctt 1980
ctttaaggcg ctcctgaaac tccgcgtcgg cgtggttgta cttgaggtac gggttgtctc 2040
cgctgtcgag ctgccggtcg taggctttgt cgtgctcgag ggccgcggcg tctgcctcgt 2100
tgaccggctc tcccttgtcg agtccgttga agggtccgag gtacttgtac ccaggaagca 2160
caagacccct gctgtcgtcc ttatgccgct ctgcgggctt tggtggtggt gggccaggtt 2220
tgagcttcca ccactgtctt attccttcag agagagtgtc ctcgagccaa tctggaagat 2280
aaccatcggc agccatacct gatttaaatc atttattgtt caaagatgca gtcatccaaa 2340
tccacattga ccagatcgca ggcagtgcaa gcgtctggca cctttcccat gatatgatga 2400
atgtagcaca gtttctgata cgcctttttg acgacagaaa cgggttgaga ttctgacacg 2460
ggaaagcact ctaaacagtc tttctgtccg tgagtgaagc agatatttga attctgattc 2520
attctctcgc attgtctgca gggaaacagc atcagattca tgcccacgtg acgagaacat 2580
ttgttttggt acctgtctgc gtagttgatc gaagcttccg cgtctgacgt cgatggctgc 2640
gcaactgact cgcgcacccg tttgggctca cttatatctg cgtcactggg ggcgggtctt 2700
ttcttggctc cacccttttt gacgtagaat tcatgctcca cctcaaccac gtgatccttt 2760
gcccaccgga aaaagtcttt gacttcctgc ttggtgacct tcccaaagtc atgatccaga 2820
cggcgggtga gttcaaattt gaacatccgg tcttgcaacg gctgctggtg ttcgaaggtc 2880
gttgagttcc cgtcaatcac ggcgcacatg ttggtgttgg aggtgacgat cacgggagtc 2940
gggtctatct gggccgagga cttgcatttc tggtccacgc gcaccttgct tcctccgaga 3000
atggctttgg ccgactccac gaccttggcg gtcatcttcc cctcctccca ccagatcacc 3060
atcttgtcga cacagtcgtt gaagggaaag ttctcattgg tccagttgac gcagcaaggg 3120
cgaattcgtt taaacctgca ggactagtcc ctttagtgag gg 3162
<210>167
<211>3164
<212>DNA
<213>新AAV血清型,克隆hu140.2
<400>167
gcgaattgaa tttagcggcc gcgaattcgc ccttcgcaga gaccaaagtt caactgaaac 60
gaattaaacg gtttattgat taacaagcaa ttacaaatta cgagtcaggt atctggtgcc 120
aatggggcga ggctctgaat acacgccatt agtgtccaca gtaaagtcca cattaacaga 180
cttgttgtag ttggaagtgt actgaatttc gggattccag cgtttgctgt tttccttctg 240
cagctcccac tcgatctcca cgctgacctg tcccgtggag tactgtgtga tgaaggaagc 300
aaactttgcc gcactgaagg tggtcgaagg attcgcaggt accggggtgt tcttgatgag 360
aatctgtgga ggagggtgtt taggtccgaa tccacccatg aggggagagg ggtgaaaatg 420
tccgtccgtg tgtggaatct ttgcccagat gggcccctga aggtacacat ctctgtcctg 480
ccagaccatg cctggaagaa cgccttgtgt gttgacatct gcggtagctg cttgtctgtt 540
gcctctctgg aggttggtag atacagaacc atactgctcc gtagccacgg gattggttgt 600
cctgatttcc tcttcgtctg taatcatgac cttttcaatg tccacatttg ttttctctga 660
gccttgcttc ccaaagatga gaaccccgct ctgaggaaaa aacttttctt cattgtcctt 720
gtggcttgcc atggccgggc ccggattcac cagagagtct ctgccattga ggtggtactt 780
ggtagctcca gtccacgagt attcactgtt gttgttatcc gcagatgtct ttgatactcg 840
ctgctggcgg taacagggtc caggaagcca gttcctagac tggtcccgaa tgtcactcgc 900
tccggcctga gaaaactgaa gccttgactg cgtggtggtt ccacttggag tgtttgttgt 960
gctcaagtaa tacaggtact ggtcgatgag aggattcatg agacggtcca gactctggct 1020
gtgagcgtag ctgctgtgga aaggaacgtc ctcaaaagtg tagctgaagg taaagttgtt 1080
tccggtacgc agcatctgag aaggaaagta ctccgggcag taaaatgaag agcgtcctac 1140
tgcctgactc ccgttgctca gggtgaggta tccatactgt ggcaccatga agacgtctgc 1200
tgggaacggc gggaggcatc cttgatgcgc cgagccgagg acgtacggga gctggtactc 1260
cgagtcagta aacacctgaa ccgtgctggt aaggttattg gcaatcgtcg tcgtaccgtc 1320
attctgcgtg acctctttga cttgaatgtt aaagagcttg aagttgagtc tcttgggtcg 1380
gaatccccag ttgttgttga tgagtctttg ccagtcacgt ggtgaaaagt ggcagtggaa 1440
tctgttgaag tcaaaatacc cccaaggggt gctgtagcca aagtagtgat tgtcgttcga 1500
ggctcctgat tggctggaaa tttgtctgta gaggtggttg ttgtaggtgg gcagggccca 1560
ggttcgggtg ctggtggtga tgactctgtc gcccatccat gtggaatcgc aatgccgatt 1620
tcccgaggaa ttacccactc cgtcggcgcc ctcgttattg tctgccattg gtgcgccact 1680
gcctgtagcc atcgtattag ttcccagacc agagggggct gctggtggct gtccgagagg 1740
ctgggggtca ggtactgagt ctgcgtctcc agtctgacca aaattcaatc tttttcttgc 1800
aggctgctgg cccgcctttc cggttcccga ggaggagtct ggctccacag gagagtgctc 1860
taccggcctc ttttttcccg gagccgtctt aacaggttcc ccaaccaggc ccagaggttc 1920
aagaaccctc tttttcgcct ggaagactgc tcgtccgagg ttgcccccaa aagacgtatc 1980
ttctttaagg cgctcctgaa actccgcgtc ggcgtggttg tacttgaggt acgggttgtc 2040
tccgctgtcg agctgccggt cgtaggcttt gtcgtgctcg agggccgcgg cgtctgcctc 2100
gttgaccggc tctcccttgt cgagtccgtt gaagggtccg aggtacttgt acccaggaag 2160
cacaagaccc ctgctgtcgt ccttatgccg ctctgcgggc tttggtggtg gtgggccagg 2220
tttgagcttc caccactgtc ttattccttc agagagagtg tcctcgagcc aatctggaag 2280
ataaccatcg gcagccatac ctgatttaaa tcatttattg ttcaaagatg cagtcatcca 2340
aatccacatt gaccagatcg caggcagtgc aagcgtctgg cacctttccc atgatatgat 2400
gaatgtagca cagtttctga tacgcctttt tgacgacaga aacgggttga gattctgaca 2460
cgggaaagca ctctaaacag tctttctgtc cgtgagtgaa gcagatattt gaattctgat 2520
tcattctctc gcattgtctg cagggaaaca gcatcagatt catgcccacg tgacgagaac 2580
atttgttttg gtacctgtct gcgtagttga tcgaagcttc cgcgtctgac gtcgatggct 2640
gcgcaactga ctcgcgcacc cgtttgggcc cacttatatc tgcgtcactg ggggcgggtc 2700
ttttcttggc tccacccttt ttgacgtaga attcatgctc cacctcaacc acgtgatcct 2760
ttgcccaccg gaaaaagtct ttgacttcct gcttggtgac cttcccaaag tcatgatcca 2820
gacggcgggt gagttcaaat ttgaacatcc ggtcttgcaa cggctgctgg tgttcgaagg 2880
tcgttgagtt cccgtcaatc acggcgcaca tgttggtgtt ggaggtgacg atcacgggag 2940
tcgggtctat ctgggccgag gacttgcatt tctggtccac gcgcaccttg cttcctccga 3000
gaatggcttt ggccgactcc acgaccttgg cggtcatctt cccctcctcc caccagatca 3060
ccatcttgtc gacacagtcg ttgaagggaa agttctcatt ggtccagttg acgcagcaag 3120
ggcgaattcg tttaaacctg caggactagt ccctttagtg aggg 3164
<210>168
<211>3159
<212>DNA
<213>新AAV血清型,克隆hu147.2
<400>168
gattgattta gcggccgcga attcgccctt gctgcgtcaa ctggaccaat gagaactttc 60
ccttcaacga ttgcgtcgac aagatggtga tctggtggga ggagggaaag atgaccgcca 120
aggtcgtgga gtcggccaaa gccattctcg gaggaagcaa ggtgcgtgtg gaccaaaagt 180
gcaagtcttc ggcccagatc gacccgactc ccgtgatcgt cacctccaac accaacatgt 240
gcgccgtgat tgatggaaac tcaacgacct tcgagcacca gcagccgttg caagaccgga 300
tgttcaaatt tgaacttacc cgccgtctgg atcatgactt tggaaaggtc accaagcagg 360
aagtgaaaga ctttttccgg tgggcaaagg atcacgtggt tgaggtggag catgagttct 420
acgtcaaaaa gggtggagcc aaaaaaagac ccgcccccag tgacgcagat ataagtgagc 480
ccaaacgggc gcgcgagtca gttgcgcagc catcgacgtc agacgcggaa gcttcgatca 540
actacgcgga caggtaccaa aacaaatgtt ctcgtcacgt gggcatgaat ctgatgctgt 600
ttccctgcag acaatgcgag agaatgaatc agaattcaaa tatctgcttc actcacggac 660
agaaagactg tccatagtgc tttcccgtgt cagaatctca acccgtttct gtcgtcaaaa 720
aggcgtatca gaaactgtgc tacattcatc acatcatggg aaaggtgcca gacgcttgca 780
ctgcttgcga cctggtcaat gtggatttgg atgactgcat ctctgaacaa taaatgattt 840
aaatcaggta tggctgccga tggttatctt ccagattggc tcgaggacac tctctctgaa 900
ggaataagac agtggtggaa gctcaaacct ggcccaccac caccaaagcc cgcagagcgg 960
cataaggacg acagcagggg tcttgtgctt cctggataca agtacctcgg acccttcaac 1020
ggactcgaca agggagagcc ggtcaacgag gcagacgccg cggccctcga gcacgacaag 1080
gcctacgacc ggcagctcga cagcggagac aacccgtacc tcaagtacaa ccacgccgac 1140
gcggagtttc aggagcgcct taaagaagat acgtcttttg ggggcaacct cggacgagca 1200
gtcttccagg cgaaaaagag ggttcttgaa cctctgggcc tggttgagga acctgttaag 1260
acggctccgg gaaaaaagag gccggtagag cactctcctg tggagccaga ctcctcctcg 1320
ggaaccggaa aagcgggcaa ccagcctgca agaaaaagat tgaatttcgg tcagactgga 1380
gacgcagact ccgtacctga cccccagcct ctcggacagc caccagcatc cccctctggt 1440
ctgggaacta atacgatggc tacaggcagt ggcgcaccaa tggcagacaa taacgagggc 1500
gccgacggag tgggtaattc ctcgggaaat tggcattgcg attccacatg gatgggcgac 1560
agagtcgtca ccaccagcac ccgcacctgg gccctgccca cctacaacaa ccacctctac 1620
aagcagattt ccagccaatc aggagccagc aacgacaacc actactttgg ctacagcacc 1680
ccttgggggt attttgactt caacagattc cactgccact tttcgccacg cgactggcag 1740
agactcatca acaacaactg gggattccgg cccaaaagac tcaacttcaa gctgtttaac 1800
attcaagtca aggaggtcac gcagaatgac ggtacgacga cgattgccaa taaccttacc 1860
agcacggttc aggtgtttac tgacttggag taccagctcc cgtacgtcct cggctcggcg 1920
catcaaggat gcctcccgcc gttcccagca gacgtcttca tggtgccaca gtatggatac 1980
ctcaccctga acaacgggag tcaggcggta ggacgctctt ccttttactg cctggagtac 2040
tttccttctc agatgcttcg caccggaaac aactttacct tcagctacac ttttgaagac 2100
gttcctttcc acagcagcta cgctcacagt caaagtctgg accgtctcat gaatcctctc 2160
atcgaccagt acctgtatta cttgagcaga acaaacactc caagcggaac cactacgcag 2220
tccaggcttc agttttctca ggccggagcg agtgacattc gggaccagtc taggaactgg 2280
cttcctggac cctgttaccg ccagcagcga gtatcaaaga cagctgcgga taacaacaac 2340
agtgaatact cgtggactgg agctaccaag taccacctca atggcagaga ctctctggtg 2400
aatccgggcc cggccatggc cagccacaag gacgatgaag aaaagttttt tcctcaaagc 2460
ggggttctca tctttgggaa gcaaggctca gagaaaacaa atgtggacat tgaaaaggtc 2520
atgattacag acgaagagga aatcaggacc accaatcccg tggctacgga gcagtatggt 2580
tctgtatcta ccaacctcca gagcggcaac acacaagcag ctacctcaga tgtcaacaca 2640
caaggcgttc ttccaggcat ggtctggcag gacagagacg tgtacctgca ggggcccatc 2700
tgggcaaaaa ttccacacac ggacggacat tttcacccct ctcccctcat gggcggattt 2760
ggacttaaac accctcctcc acagattctc attaagaata ccccggtacc tgcgaatcct 2820
tcgaccacct tcagcgcggc aaagtttgct tccttcatca cacagtattc cacggggcag 2880
gtcagcgtgg agatcgagtg ggagctgcag aaggagaaca gcaaacgctg gaatcccgaa 2940
attcagtaca cttccaacta caacaaatct gttaatgtgg actttactgt ggacactaat 3000
ggggtgtatt cagagcctcg ccctattggc accagatacc tgactcgtaa tctgtaattg 3060
cttgttaatc aataaaccgt ttaattcgtt tcagttgaac tttggtctct gcgaagggcg 3120
aattcgttta aacctgcagg actagtccct ttagtgagg 3159
<210>169
<211>3156
<212>DNA
<213>新AAV血清型,克隆hu147.3
<400>169
cgattgaatt tagcggccgc gaattcgccc ttgctgcgtc aacggaccaa tgagaacttt 60
cccttcaacg attgcgtcga caagatggtg atctggtggg aggagggaaa gatgaccgcc 120
aaggtcgtgg agtcggccaa agccattctc ggaggaagca aggtgcgtgt ggaccaaaag 180
tgcaagtctt cggcccagat cgacccgact cccgtgatcg tcacctccaa caccaacatg 240
tgcgccgtga ttgatggaaa ctcaacgacc ttcgagcacc agcagccgtt gcaagaccgg 300
atgttcaaat ttgaacttac ccgccgtctg gatcatgact ttggaaaggt caccaagcag 360
gaagtgaaag actttttccg gtgggcaaag gatcacgtgg ttgaggtgga gcatgagttc 420
tacgtcaaaa agggtggagc caaaaaaaga cccgccccca gtgacgcaga tataagtgag 480
cccaaacggg cgcgcgagtc agttgcgcag ccatcgacgt cagacgcgga agcttcgatc 540
aactacgcgg acaggtacca aaacaaatgt tctcgtcacg tgggcatgaa tctgatgctg 600
tttccctgca gacaatgcga gcgaatgaat cagaattcaa atatctgctt cactcacgga 660
cagaaagact gtttagagtg ctttcccgtg tcagaatctc aacccgtttc tgtcgtcaaa 720
aaggcgtatc agaaactgtg ctacattcat cacatcatgg gaaaggtgcc agacgcttgc 780
actgcttgcg acctggtcaa tgtggatttg gatgactgca tctctgaaca ataaatgatt 840
taaatcaggt atggctgccg atggttatct tccagattgg ctcgaggaca ctctctctga 900
aggaataaga cagtggtgga agctcaaacc tggcccacca ccaaagcccg cagagcggca 960
taaggacgac agcaggggtc ttgtgcttcc tggatacaag tacctcggac ccttcaacgg 1020
actcgacaag ggagagccgg tcaacgaggc agacgccgcg gccctcgagc acgacaaggc 1080
ctacgaccgg cagctcgaca gcggagacaa cccgtacctc aagtacaacc acgccgacgc 1140
ggagtttcag gagcgcctta aagaagatac gtcttttggg ggcaacctcg gacgagcagt 1200
cttccaggcg aaaaagaggg ttcttgaacc tctgggcctg gttgaggaac ctgttaagac 1260
ggctccggga aaaaagaggc ccgtagagca ctctcctgtg gagccagact cctcctcggg 1320
aaccggaaaa gcgggcaacc agcctgcaag aaaaagattg aatttcggtc agactggaga 1380
cgcagactcc gtacctgacc cccagcctct cggacagcca ccagcagccc cctctggtct 1440
gggaactaat acgatggcta caggcagtgg cgcaccaatg gcagacaata acgagggcgc 1500
cgacggagtg ggtaattcct cgggagattg gcattgcgat tccacatgga tgggcgacag 1560
agtcatcacc accagcaccc gcacctgggc cctgcccacc tacaacaacc acctctacaa 1620
gcagatttcc agccaatcag gagccagcaa tgacaaccac tactttggct acagcacccc 1680
ttgggggtat tttgacttca acagattcca ctgccacttt tcgccacgcg actggcagag 1740
actcatcaac aacaactggg gattccggcc caaaagactc aacctcaagc tgtttaacat 1800
tcaagtcaag gaggtcacgc agaatgacgg tacgacgacg attgccaata accttaccag 1860
cacggttcag gtgtttactg acttggagta ccagctcccg tacgtcctcg gctcggcgca 1920
tcaaggatgc ctcccgccgt tcccagcaga cgtcttcatg gtgccacagt atggatacct 1980
caccctgaac aacgggagtc aggcggtagg acgctcttcc ttttactgcc tggagtactt 2040
tccttctcag atgcttcgta ccggaaacaa ctttaccttc agctacactt ttgaagacgt 2100
tcctttccac agcagctacg ctcacagtca aagtctggac cgtctcatga atcctctcat 2160
cgaccagtac ctgtattact tgagcagaac aaacactcca agcggaacca ctacgcagtc 2220
caggcttcag ttttctcagg ccggagcgag tgacattcgg gaccagtcta ggaactggct 2280
tcctggaccc tgttaccgcc agcagcgagt atcaaagaca gctgcggata acaacaacgg 2340
tgaatactcg tggactggag ctaccaagta ccacctcaat ggcagagact ctctggtgaa 2400
tccgggcccg gccatggcca gccacaagga cgatgaagaa aagttttttc ctcaaagcgg 2460
ggttctcatc tttgggaagc aaggctcaga gaaaacaaat gtggacattg aaaaggtcat 2520
gattacagac gaagaggaaa tcaggaccac caatcccgtg gctacggagc agtatggttc 2580
tgtatctacc aacctccaga gcggcaacac acgagcagct acctcagatg tcaacacaca 2640
aggcgttctt ccaggcatgg tctggcagga cagagacgtg tacctgcagg ggcccatctg 2700
ggcaaaaatt ccacacacgg acggacattt tcacccctct cccctcatgg gcggatttgg 2760
acttaaacac cctcctccac agattctcat taagaatacc ccggtacctg cgaatccttc 2820
gaccaccttc agcgcggcaa agtttgcttc cttcatcaca cagtattcca cggggcaggt 2880
cagcgtggag atcgagtggg agctgcagaa ggagaacagc aaacgctgga atcccgaaat 2940
tcagtacact tccaactaca acaaatctgt taatgtggac tttactgtgg acactaatgg 3000
ggtgtattca gagcctcgcc ctattggcac cagatacctg actcgtaatc tgtaattgct 3060
tgttaatcaa taaaccgttt aattcgtttc agttgaactt tggtctctgc gaagggcgaa 3120
ttcgtttaaa cctgcaggac tagtcccttt agtgag 3156
<210>170
<211>3158
<212>DNA
<213>新AAV血清型,克隆hu161.10
<400>170
gattgaattt agcggccgcg aattcgccct tgctgcgtca actggaccaa tgagaacttt 60
cctttcaatg attgcgtcga caagatggtg atctggtggg aggagggaaa gatgaccgcc 120
aaggtcgtgg agtcggccaa agccattctc ggaggaagca aggtgcgcgc ggaccagaaa 180
tgcaagtcct cggcccagat agacccgact cccgtgattg tcacctccaa caccaacatg 240
tgcgccgtga ttgacgggaa ctcaacgacc ttcgaacacc agcagccgtt gcaagaccgg 300
atgttcaaat ttgaactcac ccgccgtctg gatcatgact ttgggaaggt caccaagcag 360
gaagtcaaag actttttccg gtgggcaaag gatcacgtgg ttgaggtgga gcatgaattc 420
tacgtcaaaa agggtggagc taagaaaaga cccgccccca gtgacgcaga tataagtgag 480
cccaaacggg cgcgcgagtc agttgcgcag ccatcgacgt cagacgcgga agcttcgatc 540
aactacgcgg gcaggtacca aaacaaatgt tctcgtcacg tgggcatgaa tctgatgctg 600
tttccctgca gacaatgcga gagaatgaat cagaattcaa atatctgctt cactcacgga 660
cagaaagact gtttagagtg ctttcccgtg tcagaatctc aacccgtttc tgtcgtcaaa 720
aaggcgtatc agaaactttg ctacattcat catatcatgg gaaaggtgcc agacgcttgc 780
actgcctgcg atctggtcaa tgtggatttg gatgactgca tctctgaaca ataaatgatt 840
taaatcaggt atggctgccg atggttatct tccagattgg ctcgaggaca ctctctctga 900
aggaataaga cagtggtgga agctcaaacc tggcccacca ccaccaaagc ccgcagagcg 960
gcataaggac gacagcaggg gtcttgtgct tcctgggtac aagtacctcg gacccttcaa 1020
cggactcgac aagggggagc cggtcaacga ggcagacgcc gcggccctcg agcacgacaa 1080
ggcctacgac cggcagctcg acagcggaga caacccgtac ctcaagtaca accacgccga 1140
cgcggagttt caggagcgcc ttaaagaaga tacgtctttt gggggcaacc tcggacgagc 1200
agtcttccag gcaaaaaaga gggttcttga acctctgggc ctggttgagg aacctgttaa 1260
aacggctccg ggaaaaaaga ggccggtaga gcactctcct gtggagccag actcctcctc 1320
gggaaccgga aaagcgggcc agcagcctgc aagaaaaaga ttgaatttcg gtcagactgg 1380
agacgcagac tccgtacctg acccccagcc tctcggacag ccaccagcag ccccctctgg 1440
tctgggatct actacaatgg ctacaggcag tggcgcacca atggcagaca ataacgaggg 1500
tgccgatgga gtgggtaatt cctcgggaaa ttggcattgc gattcccaat ggctgggcga 1560
cagagtcatc accaccagca cccgcacctg ggccctgccc acctacaaca accacctcta 1620
caagcaaatc tccagccaat caggagcctc aaacgacaac cactactttg gctacagcac 1680
cccctggggg tattttgact tcaacagatt ccactgccac ttttcaccac gtgactggca 1740
aagactcatc aacaacaact ggggattccg acccaagaga ctcaacttca agctctttaa 1800
cattcaagtc aaagaggtca cgcagaatga cggtacgacg acgattgcca ataaccttac 1860
cagcacggtt caggtgttta ctgactcgga gtaccagctc ccgtacgtcc tcggctcggc 1920
gcatcaagga tgcctcccgc cgtttccagc ggacgtcttc atggtcccac agtatggata 1980
cctcaccctg aacaacggga gtcaggcagt aggacgctct tcattttact gcctggagta 2040
ctttccttct cagatgctgc gtaccggaaa caactttcaa ttcagctaca cttttgaaga 2100
cgtgcctttc cacagcagct acgctcacag ccagagtctg gatcggctga tgaatcctct 2160
ggtcgaccag tacctgtatt atctgaacaa gacacaaaca aatagtggaa ctcttcagca 2220
gtctcggcta ctgtttagcc aagctggacc caccaacatg tctcttcaag ctaaaaactg 2280
gctgcctgga ccttgctaca gacagcagcg tctgtcaaag caggcaaacg acaacaacaa 2340
cagcaacttt ccctggactg cagctacaaa gtatcatcta aatggccggg actcgttggt 2400
taatccagga ccagctatgg ccagtcacaa ggatgacgaa gaaaagtttt tccccatgca 2460
tggaaccctt atatttggta aacaaggaac aaatgccaac gacgcggatt tggaaaatgt 2520
catgattaca gatgaagaag aaatcaggac caccaatccc gtggctacgg agcagtacgg 2580
aactgtatca aataatttgc aaaactcaaa cactggtcca actactggaa ctgtcaatca 2640
ccaaggagcg ttacctggta tggtgtggca ggatcgagac gtgtacctgc agggacccat 2700
ttgggccaag attcctcaca ccgatggaca ctttcatcct tctccactga tgggaggttt 2760
tggactcaaa cacccacctc ctcaaatcat gatcaaaaac actcccgttc cagccaatcc 2820
tcccacaaac ttcagttctg ccaagtttgc ttctttcatc acacagtatt ccacggggca 2880
ggtcagcgtg gagatcgagt gggagctgca gaaggagaac agcaaacgct ggaatcccga 2940
aattcagtac acttccaact acaacaaatc tgttaatgtg gactttactg tggacactaa 3000
tggtgtgtat tcagagcctc gccccattgg caccagatac ctgactcgta atctgtaatt 3060
gcttgttaat caataaaccg tttaattcgt ttcagttgaa ctttggtctc tgcgaagggc 3120
gaattcgttt aaacctgcag gactagtccc tttagtga 3158
<210>171
<211>3167
<212>DNA
<213>新AAV血清型,克隆hu172.1
<400>171
attgaattta gcggccgcga attcgccctt cgcagagacc aaagttcaac tgaaacgaat 60
taaacggttt attgattaac aagcaattac agattacgag tcaggtacct ggtgccaatg 120
gggcgaggct ctgaatacac accattagtg tccacagtaa agtccacatt aacagatttg 180
ttgtagttgg aagtgtactg aatctcggga ttccagcgtt tgctgttctc cttctgcagc 240
tcccactcga tctccacgct gacctgcccc gtggagtact gtgtaatgaa ggaagcaaac 300
tttgccgcac tgaaggtagt cgaaggattc gcaggtaccg gggtgttctt gatgagaatc 360
tgcgggggag ggtgtttaag tccgaatccg cccatgaggg gagaggggtg aaaatgtccg 420
tccgtgtgtg gaatctttgc ccagatgggc ccctgcaggt acacgtctct gtcctgccac 480
accatgcctg gaagaacgcc ttgtgtgttg acatctgagg tagctgcttg tgtgttgccg 540
ctctggaggt tggtagatac agaaccatac tgctccgtag ccacgggatt ggtggtcctg 600
atttcctctt cgtctgtaat catgaccttt tcaatgtcca cattagtttt tcccgagtct 660
tgttttccaa agatgagaac cccgctctga ggaaaaaact tttcttcatc gtccttgtgg 720
ctggccatgg ccgggcccgg attcaccaga gagtctcttc cattaaggtg gtacttggta 780
gctccagtcc acgagtattc actgttgttg ttatccgcag atgtctttga tactcgctgc 840
tggcggtaac agggtccagg aagccagttc ctagactggt cccgaatgtc acttgctccg 900
gcctgagaaa actgaagcct tgactgcgtg gtggttccgc ttggagtgtt tgttctgctc 960
aagtaataca ggtactggtc gatgagagga ttcatgagac ggtccaaact ctggctgtgg 1020
gcgtagctgc tgtggaaagg aacgtcctca aaggtgtagc tgaaggtaaa gttgtttccg 1080
gtacgcagca tctgagaagg aaagtactcc aggcagtaaa atgaagagcg tcctactgcc 1140
tgactcccgt tgttcagggt gaggtatcca tactgtggca ccatgaagac gtctgctggg 1200
aacggcggga ggcatccttg atgcgccgag ccgaggacgt acgggagctg gtactccgag 1260
tcagtaaaca cctgaaccgt gctggtaagg ttattggcaa tcgtcgtcgt accgtcattc 1320
tgcgtgacct ccttgacttg aatgttaaag agcttgaagt tgagtctttt gggccggaat 1380
ccccaattgt tgttgatgag tctttgccag tcacgtggcg aaaagtggca gtggaatctg 1440
ttgaagtcaa aataccccca aggggtgctg tagccaaagt agtggttgtc gtttgaggct 1500
cctgattggc tggaaatctg cttgtagagg tggttgttgt aggtgggcag agcccaggtg 1560
cgggtgctgg tggtgatgac tctgtcgccc atccatgtgg aatcgcaatg ccaatttccc 1620
gaggaattac ccactccgtc ggcgccctcg ttattgtctg ccattggtgc gccactgcct 1680
gtagccatcg tattagttcc cagaccagag ggggctgctg gtggctgtcc gagaggctgg 1740
gggtcaggta cggagtctgc gtctccagtc tgaccgaaat tcaatctctt tcttgcaggc 1800
tgctggcccg cttttccggt tcccgaggag gagtctggct ccgcaggaga gtgctctacc 1860
ggcctctttt ttcccggagc cgtcttaaca ggttcctcaa ccaggcccag aggttcaaga 1920
accctctttt tcgcctggaa gactgctcgt ccgaggttgc ccccaaaaga cgtatcttct 1980
ttaagacgct cctggaactc cgcgtcggcg tggttgtact tggggtacgg gttgtctccg 2040
ctgtcgagct gccggtcgta ggccttgtcg tgctcgaggg ccgcggcgtc tgcctcgttg 2100
accggctctc ccttgtcgag tccgttgaag ggtccaaggt acttgtaccc aggaagcaca 2160
agacccctgc tgtcgtcctt atgccgctct gcgggctttg gtggtggtgg gccaggtttg 2220
agcttccacc actgtcttat tccttcagag agagtgtcct cgagccaatc tggaagataa 2280
ccatcggcag ccatacctga tttaaatcat ttattgttca gagatgcagt catccaaatc 2340
cacattgacc agatcgcaag cagtgcaagc gtctggcacc tttcccatga tatgatgaat 2400
gtagcacagt ttctgatacg cctttttgac gacagaaacg ggttgagatt ctgacacggg 2460
aaagcactct aaacagtctt tctgtccgtg agtgaagcag atatttgaat tctgattcat 2520
tctctcgcat tgtctgcagg gaaacagcat cagattcatg cccacgtgac gagaacattt 2580
gttttggtac ctgtccgcgt agttgattga agcttccgcg tctgacgtcg atggctgcgc 2640
aactgactcg cgcgcccgtt tgggctcact tatatctgcg tcactggggg cgggtctttt 2700
tttggctcca ccctttttga cgtagaattc atgctctacc tcaaccacgt gatcctttgc 2760
ccaccggaaa aagtctttga cttcctgctt ggtgaccttc ccaaagtcat gatccagacg 2820
gcgggtgagt tcaaatttga acatccggtc ttgcaacggc tgctggtgtt cgaaggtcgt 2880
tgagttcccg tcaatcactg cgcacatgtt ggtgttggag gtgacaatca cgggagtcgg 2940
gtctatctgg gccgaggact tgcatttctg gtccacgcgc accttgcttc ctccgagaat 3000
ggctttggcc gactccacga ccttggcggt catcttcccc tcctcccacc agatcaccat 3060
cttgtcgacg caatcattga aaggaaagtt ctcattggtc cagttgacgc agccgtagaa 3120
agggcgaatt cgtttaaacc tgcaggacta gtccctttag tgagggt 3167
<210>172
<211>3161
<212>DNA
<213>新AAV血清型,克隆hu172.2
<400>172
aattgaattt agcggccgcg aattcgccct tcgcagagac caaagttcaa ctgaaacgaa 60
ttaaacggtt tattgattaa caagcaatta cagattacga gtcaggtatc tggtgccaat 120
ggggcgaggc tctgaataca caccattagt gtccacagta aagtccacat taacagattt 180
gttgtagttg gaagtgtact gaatctcggg attccagcgt ttgctgttct ccttctgcag 240
ctcccactcg atctccacgc tgacctgccc cgtggagtac tgtgtaatga aggaagcaaa 300
ctttgccgca ctgaaggtag tcgaaggatt cgcaggtacc ggggtgttct tgatgagaat 360
ctgcggggga gggtgtttaa gtccgaatcc gcccatgagg ggagaggggt gaaaatgtcc 420
gtccgtgtgt ggaatctttg cccagatggg cccctgcagg tacacgtctc tgtcctgcca 480
caccatgcct ggaagaacgc cttgtgtgtt gacatctgag gtagctgctt gtgtgttgcc 540
gctctggagg ttggtagata cagaaccata ctgctccgta gccacgggat tggtggtcct 600
gatttcctct tcgtctgtaa tcatgacctt ttcaatgtcc acattagttt ttcccgagtc 660
ttgttttcca aagatgagaa ccccgctctg aggaaaaaac ttttcttcat cgtccttgtg 720
gctggccatg gccgggcccg gattcaccag agagtctctt ccattaaggt ggtacttggt 780
agctccagtc cacgagtatt cactgttgtt gttatccgca gatgtctttg atactcgctg 840
ctggcggtaa cagggtccag gaagccagtt cctagactgg tcccgaatgt cacttgctcc 900
ggcctgagaa aactgaagcc ttgactgcgt ggtggttccg cttggagtgt ttgttctgct 960
caagtaatac aggtactggt cgacgagagg attcatgaga cggtccaaac tctggctgtg 1020
ggcgtagctg ctgtggaaag gaacgtcctc aaaggtgtag ctgaaggtaa agttgtttcc 1080
ggtacgcagc atctgagaag gaaagtactc caggcagtaa aatgaagagc gtcctactgc 1140
ctgactcccg ttgttcaggg tgaggtatcc atactgtggc accatgaaga cgtctgctgg 1200
gaacggcggg aggcatcctt gatgcgccga gccgaggacg tacgggagct ggtactccga 1260
gtcagtaaac acctgaaccg tgctggtaag gttattggca atcgtcgtcg taccgtcatt 1320
ctgcgtgacc tccttgactt gaatgttaaa gagcttgaag ttgagtcttt tgggccggga 1380
tccccaattg ttgttgatga gtctttgcca gtcacgtggc gaaaagtggc agtggaatct 1440
gttgaagtca aaataccccc aaggggtgct gtagccaaag tagtggttgt cgtttgaggc 1500
tcctgattgg ctggaaatct gcctgtagag gtggttgttg taggtgggca gagcccaggt 1560
gcgggtgctg gtggtgatga ctctgtcgcc catccatgtg gaatcgcaat gccaatttcc 1620
cgaggaatta cccactccgt cggcgccctc gttattgtct gccattggtg cgccactgcc 1680
tgtagccatc gtattagttc ccagaccaga gggggctgct ggtggctgtc cgagaggctg 1740
ggggtcaggt acggagtctg cgtctccagt ctgaccgaaa ttcaatctcc ttcttgcagg 1800
ctgctggccc gcttttccgg ttcccgagga ggagtctggc tccgcaagag agtgctctac 1860
cggcctcttt tttcccggag ccgtcttaac aggttcctca accaggccca gaggttcaag 1920
aaccctcttt ttcgcctgga agactgctcg tccgaggttg cccccaaaag acgtatcttc 1980
tttaagacgc tcctggaact ccgcgtcggc gtggttgtac ttgaggtacg ggttgtctcc 2040
gccgtcgagc tgccggtcgt aggccttgtc gtgctcgagg gccgcggcgt ctgcctcgtt 2100
gaccggctct cccttgtcga gtccgttgaa gggtccaagg tacttgtacc caggaagcac 2160
aagacccctg ctgtcgtcct tatgccgctc tgcgggcttt ggtggtggtg ggccaggttt 2220
gagcttccac cactgtctta ttccttcaga gagagtgtcc tcgagccaat ctggaagata 2280
accatcggca gccatacctg atttaaatca tttattgttc agagatgcag tcatccaaat 2340
ccacattgac cagatcgcaa gcagtgcaag cgtctggcac ctttcccatg atatgatgaa 2400
tgtagcacag tttctgatac gcctttttga cgacagaaac gggttgagat tctgacacgg 2460
gaaagcactc taaacagtct ttctgtccgt gagtgaagca gatatttgaa ttctgattca 2520
ttctctcgca ttgtctgcag ggaaacagca tcagattcat gcccacgtga cgagaacatt 2580
tgttttggta cctgtccgcg tagttgattg aagcttccgc gtctgacgtc gatggctgcg 2640
caactgactc gcgcgcccgt ttgggctcac ttatatctgc gtcactgggg gcgggtcttt 2700
ttttggctcc accctttttg acgtagaatt catgctctac ctcaaccacg tgatcctttg 2760
cccaccggaa aaagtctttg acttcctgct tggtgacctt cccaaagtca tgatccagac 2820
ggcgggtgag ttcaaatttg aacatccggt cttgcaacgg ctgctggtgt tcgaaggtcg 2880
ttgagttccc gtcaatcact gcgcacatgt tggtgttgga ggtgacaatc acgggagtcg 2940
ggtctatctg ggccgaggac ttgcatttct ggtccacgcg caccttgctt cctccgagaa 3000
tggctttggc cgactccacg accttggcgg tcatcttccc ctcctcccac cagatcacca 3060
tcttgtcgac gcaatcattg aaaggaaagt tctcattggt ccagttgacg cagcaagggc 3120
gaattcgttt aaacctgcag gactagtccc tttagtgagg g 3161
<210>173
<211>3172
<212>DNA
<213>新AAV血清型,克隆hu173.4
<400>173
gattgaattt agcggccgcg aattcgccct tgctgcgtca actggaccaa tgagaacttt 60
cccttcaacg attgcgtcga caagatggtg atctggtggg aggagggcaa gatgaccgcc 120
aaggtcgtgg agtccgccaa ggccattctg ggtggaagca aggtgcgcgt ggaccaaaag 180
tgcaagtcat cggcccagat cgaccccacg cccgtgatcg tcacctccaa caccaacatg 240
tgcgccgtga tcgacgggaa cagcaccacc ttcgagcacc agcagcccct gcaggaccgc 300
atgttcaagt tcgagctcac ccgccgtctg gagcacgact ttggcaaggt gaccaagcag 360
gaagtcaaag agttcttccg ctgggctcag gatcacgtga ctgaggtggc gcatgagttc 420
tacgtcagaa agggcggagc caccaaaaga cccgccccca gtgacgcgga tataagcgag 480
cccaagcggg cctgcccctc agttgcggag ccatcgacgt cagacgcgga agcaccggtg 540
gactttgcgg acaggtacca aaacaaatgt tctcgtcacg cgggcatgct tcagatgctg 600
tttccctgca agacatgcga gagaatgaat cagaatttca acgtctgctt cacgcacggg 660
gtcagagact gctcagagtg cttccccggc gcgtcggaat ctcaacccgt cgtcagaaaa 720
aagacgtatc agaaactgtg cgcgattcat catctgctgg ggcgggcacc cgagattgcg 780
tgttcggcct gcgatctcgt caacgtggac ttggatgact gtgtttctga gcaataaatg 840
acttaaacca ggtatggctg ctgacggtta tcttccagat tggctcgagg acaacctctc 900
tgagggcatt cgcgagtggt gggacctgaa acctggagcc cccaagccca aggccaacca 960
gcagaagcag gacgacggcc ggggtctggt gcttcctggc tacaagtacc tcggaccctt 1020
caacggactc gacaaggggg agcccgtcaa cgcggcggac gcagcggccc tcgagcacga 1080
caaggcctac gaccagcagc tcaaagcggg tgacaatccg tacctgcggt ataaccacgc 1140
cgacgccgag tttcaggagc gtctgcaaga agatacgtct tttgggggca acctcgggcg 1200
agcagtcttc caggccaaga agcgggttct cgaacctctc ggtctggttg aggaagctgc 1260
taagacggct cctggaaaga agagaccggt agaaccgtca cctcagcgtt cccccgactc 1320
ctccgcgggc atcggcaaga aaggccagca gcccgctaaa aagagactga actttggtca 1380
gactggcgac tcagagtcag tccccgaccc tcaaccaatc ggagaaccac cagcaggccc 1440
ctctggtctg ggatctggta caatggctgc aggcggtggc gctccaatgg cagacaataa 1500
cgaaggcgcc gacggagtgg gtagttcctc aggaaattgg cattgcgatt ccacatggct 1560
gggcgacaga gtcatcacca ccagcacccg aacctgggcc ctgcccacct acaacaacca 1620
cctctacaag caaatatcca atgggacatc gggaggaagc accaacgaca acacctactt 1680
cggctacagc accccctggg ggtattttga cttcaacaga ttccactgcc acttctcacc 1740
acgtgactgg cagcgactca tcaacaacaa ctggggattc cggccaaaaa gactcagctt 1800
caagctcttc aacatccagg tcaaggaggt cacgcagaat gaaggcaccg agaccatcgc 1860
caataacctt accagcacga ttcaggtatt tacggactcg gaataccagc tgccgtacgt 1920
cctcggctcc gcgcaccagg gctgcctgcc tccgttcccg gcggacgtct tcatgattcc 1980
ccagtacggc taccttacac tgaacaatgg aagtcaagcc gtaggccgtt cctccttcta 2040
ctgcctggaa tattttccat ctcaaatgct gcgaactgga aacaattttg aattcagcta 2100
caccttcgag gacgtgcctt tccacagcag ctgcgcacac agccagagct cggaccgact 2160
gatgaatcct ctcatcgacc agtacctgta ctacttatcc agaactcggt ccacaggagg 2220
aactcaaggt acccagcaat tgttattttc tcaagctggg cctgcaaaca tgtcggctca 2280
ggctaagaac tggctacctg gaccttgcta ccggcagcag cgagtctcta cgacactgtc 2340
gcaaaacaac aacagcaact ttgcttggac tggtgccacc aaatatcacc tgaacggaag 2400
agactctttg gtaaatcccg gtgtcgccat ggcaacccac aaggacgacg aggaacgctt 2460
cttcccgtcg agtggagtcc tgatgtttgg aaaacagggt gctggaagag acaatgtgga 2520
ctacagcagc gttatgctaa ccagcgaaga agaaattaaa accactaacc ctgtagccac 2580
agaacaatac ggtgtggtgg ctgacaactt gcagcaaacc aatacagggc ctattgtggg 2640
aaatgtcaac agccaaggag ccttacctgg catggtctgg cagaaccgag acgtgtacct 2700
gcagggtccc atctgggcca agattcctca cacggacggc aacttccacc cttcaccgct 2760
aatgggagga tttggactga agcacccacc tcctcagatc ctgatcaaga acacgccggt 2820
acctgcggat cctccaacaa cgttcagcca ggcgaaattg gcttccttca ttacgcagta 2880
cagcaccgga caggtcagcg tggaaatcga gtgggagctg cagaaggaga acagcaaacg 2940
ctggaaccca gagattcagt acacttcaaa ctactacaaa tctacaaatg tggactttgc 3000
tgtcaataca gagggaactt attctgagcc tcgccccatt ggtactcgtt acctcacccg 3060
taatctgtaa ttgctggtta atcaataaac cgtttgattc gtttcagttg aactttggtc 3120
tctgcgaagg gcgaattcgt ttaaacctgc aggactagtc cctttagtga gg 3172
<210>174
<211>3159
<212>DNA
<213>新AAV血清型,克隆hu161.8
<400>174
gattgaattt agcggccgcg aattcgccct tgctgcgtca actggaccaa tgagaacttt 60
cctttcaatg attgcgtcga caagatggtg atctggtggg aggagggaaa gatgaccgcc 120
aaggtcgtgg agtcggccaa agccattctc ggaggaagca aggtgcgcgt ggaccagaaa 180
tgcaagtcct cggcccagat agacccgact cccgtgattg tcacctccaa caccgacatg 240
tgcgccgtga ttgacgggaa ctcaacgacc ttcgaacacc agcagccgtt gcaagaccgg 300
atgttcaaat ttgaactcac ccgccgtctg gatcatgact ttgggaaggt caccaagcag 360
gaagtcaaag actttttccg gtgggcaaag gatcacgtgg ttgaggtgga gcatgaattc 420
tacgtcaaaa agggtggagc taagaaaaga cccgccccca gtgacgcaga tataagtgag 480
cccaaacggg cgcgcgagtc agttgcgcag ccatcgacgt cagacgcgga agcttcgatc 540
aactacgcgg acaggtacca aaacaaatgt tctcgtcacg tgggcatgaa tctgatgctg 600
tttccctgca gacaatgcga gagaatgaat cagaattcaa atatctgctt cactcacgga 660
cagaaagact gtttagagtg ctttcccgtg tcagaatctc aacccgtttc tgtcgtcaaa 720
aaggcgtatc agaaactttg ctacattcat catatcatgg gaaaggtgcc agacgcttgc 780
actgcctgcg atctggtcaa tgtggatttg gatgactgca tctctgaaca ataaatgatt 840
taaatcaggt atggctgccg atggttatct tccagattgg ctcgaggaca ctctctctga 900
aggaataaga cagtggtgga agctcaaacc tggcccacca ccaccaaagc ccgcagagcg 960
gcataaggac gacagcaggg gtcttgtgct tcctgggtac aagtacctcg gacccttcaa 1020
cggactcgac aagggggagc cggtcaacga ggcagacgcc gcggccctcg agcacgacaa 1080
ggcctacgac cggcagctcg acagcggaga caacccgtac ctcaagtaca accacgccga 1140
cgcggagttt caggagcgcc ttaaagaaga tacgtctttt gggggcaacc tcggacgagc 1200
agtcttccag gcaaaaaaga gggttcttga acctctgggc ctggttgagg aacctgttaa 1260
aacggctccg ggaaaaaaga ggccggtaga gcaccctcct gtggagccag actcctcctc 1320
gggaaccgga aaagcgggcc agcagcctgc aagaaaaaga ttgaatttcg gtcagactgg 1380
agacgcagac tccgtacctg acccccagcc tctcggacag ccaccagcag ccccctctgg 1440
tctgggatct actacaatgg ctacaggcag tggcgcacca atggcagaca ataacgaggg 1500
tgccgatgga gtgggtaatt cctcgggaaa ttggcattgc gattcccaat ggctgggcga 1560
cagagtcatc accaccagca cccgcacctg ggccctgccc acctacaaca accacctcta 1620
caagcaaatc tccagccaat caggagcctc aaacgacaac cactactttg gctacagcac 1680
cccctggggg tattttgact tcaacagatt ccactgccac ttttcaccac gtgactggca 1740
aagactcatc aacaacaact ggggattccg acccaagaga ctcaacttca agctctttaa 1800
cattcaagtc aaagaggtca cgcagaatga cggtacgacg acgattgcca ataaccttac 1860
cagcacggtt caggtgttta ctgactcgga gtaccagctc ccgtacgtcc tcggctcggc 1920
gcatcaagga tgcctcccgc cgtttccagc ggacgtcttc atggtcccac agtatggata 1980
cctcaccctg aacaacggga gtcaggcagt aggacgctct tcattttact gcctggagta 2040
ctttccttct cagatgctgc gtaccggaaa caactttcag ttcagctaca cttttgaaga 2100
cgtgcctttc cacagcagct acgctcacag ccagagtctg gatcggctga tgaatcctct 2160
gatcgaccag tacctgtatt atctgaacaa gacacaaaca aatagtggaa ctcttcagca 2220
gtctcggcta ctgtttagcc aagctggacc caccaacatg tctcttcaag ctaaaaaccg 2280
gctgcctgga ccttgctaca gacagcagcg tctgtcaaag caggcaaacg acaacaacaa 2340
cagcaacttt ccctggaccg cagctacaaa gtatcatcta aatggccggg actcgttggt 2400
taatccagga ccagctatgg ccagtcacaa ggatgacgaa gaaaagtttt tccccatgca 2460
tggaaccctt atatttggta aacaaggaac aaatgccaac gacgcggatt tggaaaatgt 2520
catgattaca gatgaagaag aaatcaggac caccaatccc gtggctacgg agcagtacgg 2580
aactgtatca aataatttgc aaaactcaaa cactggtcca actactggaa ctgtcaatca 2640
ccaaggagcg ttacctggta tggtgtggca ggatcgagac gtgtacctgc agggacccat 2700
ttgggccaag attcctcaca ccgatggaca ctttcatcct tctccactgg tgggaggttt 2760
tggactcaaa cacccacctc ctcaaatcat gatcaaaaac actcccgttc cagccaatcc 2820
tcccacaaac ttcagttctg ccaagtttgc ttctttcatc acacagtatt ccacggggca 2880
ggtcagcgtg gagatcgagt gggagctgca gaaggagaac agcaaacgct ggaatcccga 2940
aattcagtac acttccaact acaacaaatc tgttaatgtg gactttactg tggacactaa 3000
tggtgtgtat tcagagcctc gccccattgg caccagatac ctgactcgta atctgtaatt 3060
gcttgttaat caataaaccg tttaattcgt ttcagttgaa ctttggtctc tgcgaagggc 3120
gaattcgttt aaacctgcag gactagtccc tttagtgag 3159
<210>175
<211>3172
<212>DNA
<213>新AAV血清型,克隆hu173.8
<400>175
gattgaattt agcggccgcg aattcgccct tgctgcgtca actggaccaa tgagaacttt 60
cccttcaacg attgcgtcga caagatggtg atctggtggg aggagggcaa gatgaccgcc 120
aaggtcgtgg agtccgccaa ggccattctg ggtggaagca aggtgcgcgt ggaccaaaag 180
tgcaagtcat cggcccagat cgaccccacg cccgtgatcg tcacctccaa caccaacatg 240
tgcgccgtga tcggcgggaa cagcaccacc ttcgagcacc agcagcccct gcaggaccgc 300
atgttcaagt tcgagctcac ccgccgtctg gagcacgact ttggcaaggt gaccaagcag 360
gaagtcaaag agttcttccg ctgggctcag gatcacgtga ctgaggtggc gcatgagttc 420
tacgtcagaa agggcggagc caccaaaaga cccgccccca gtgacgcgga tataagcgag 480
cccaagcggg cctgcccctc agttgcggag ccatcgacgt cagacgcgga agcaccggtg 540
gactttgcgg acaggtacca aaacaaatgt tctcgtcacg cgggcatgct tcagatgctg 600
tttccctgca agacatgcga gagaatgaat cagaatttca acgtctgctt cacgcacggg 660
gtcagagact gctcagagtg cctccccggc gcgtcagaat ctcaacccgt cgtcagaaaa 720
aagacgtatc agaaactgtg cgcgattcat catctgctgg ggcgggcacc cgagattgcg 780
tgttcggcct gcgatctcgt caacgtggac ttggatgact gtgtttctga gcaataaatg 840
acttaaacca ggtatggctg ctgacggtta tcttccagat tggctcgagg acaacctctc 900
tgagggcatt cgcgagtggt gggacctgaa acctggagcc cccaagccca aggccaacca 960
gcagaagcag gacgacggcc ggggtctggt gcttcttggc tacaagtacc tcggaccctt 1020
caacggactc gacaaggggg agcccgtcaa tgcggcggac gcagcggccc tcgagcacga 1080
caaggcctac gaccagcagc tcaaagcggg tgacaatccg tacctgcggt ataaccacgc 1140
cgacgccgag tttcaggagc gtctgcaaga agatacgtct tttgggggca acctcgggcg 1200
agcagtcttc caggccaaga agcgggttct cgaacctctc ggtctggttg aggaagctgc 1260
taagacggct cctggaaaga agagaccggt agaaccgtca cctcagcgtt cccccgactc 1320
ctccacgggc atcggcaaga aaggccagca gcccgctaaa aagagactga actttggtca 1380
gactggcgac tcagagtcag tccccgaccc tcaaccaatc ggagaaccac cagcaggccc 1440
ctctggtctg ggatctggta caatggctgc aggcggtggc gctccaatgg cagacaataa 1500
cgaaggcgcc gacggagtgg gtagttcctc aggaaattgg cattgcgatt ccacatggct 1560
gggcgacaga gtcatcacca ccagcacccg aacctgggcc ctgcccacct acaacaacca 1620
cctctacaag caaatatcca atgggacatc gggaggaagc accaacgaca acacctactt 1680
cggctacagc accccctggg ggtattttga cttcaacaga ttccactgcc acttctcacc 1740
acgtgactgg cagcgactca tcaacaacaa ctggggattc cggccaaaaa gactcagctt 1800
caagctcttc aacatccagg tcaaggaggt cacgcagaat gaaggcacca agaccatcgc 1860
caataacctt accagcacga ttcaggtatt tacggactcg gaataccagc tgccgtacgt 1920
cctcggctcc gcgcaccagg gctgcctgcc tccgttcccg gcggacgtct tcatgattcc 1980
ccagtacggc taccttacac tgaacaatgg aagtcaagcc gtaggccgtt cctccttcta 2040
ctgcctggaa tattttccat ctcaaatgct gcgaactgga aacaattttg aattcagcta 2100
caccttcgag gacgtgcctt tccacagcgg ctacgcacac agccagagct tggaccgact 2160
gatgaatcct ctcatcgacc agtacctgta ctacttatcc agaactcagt ccacaggagg 2220
aactcaaggt acccagcaat tgttattttc tcaagctggg cctgcaaaca tgtcggctca 2280
ggctaagaac tggctacctg gaccttgcta ccggcagcag cgagtctcta cgacactgtc 2340
gcaaaacaac aacagcaact ttgcttggac tggtgccacc aaatatcacc tgaacggaag 2400
agactctttg gtaaatcccg gtgtcgccat ggcaacccac aaggacgacg aggaacgctt 2460
cttcccgtcg agtggagtcc tgatgtttgg aaaacagggt gctggaagag acaatgtgga 2520
ctacagcagc gttatgctaa ccagcgaaga agaaattaaa accactaacc ctgtagccac 2580
agaacaatac ggtgtggtgg ctgacaactt gcagcaaacc aatacagggc ctattgtggg 2640
aaatgtcaac agccaaggag ccttacctgg catggtctgg cagaaccgag acgtgtacct 2700
gcagggtccc atctgggcca agattcctca cacggacggc aacttccacc cttcaccgct 2760
aatgggagga tttggactga agcacccacc tcctcagatc ctgatcaaga acacgccggt 2820
acctgcggat cctccaacga cgttcagcca ggcgaaattg gcttccttca ttacgcagta 2880
cagcaccgga caggtcagcg tggaaatcga gtgggagctg cagaaggaga acagcaaacg 2940
ctggaaccca gagattcagt acacttcaaa ctactacaaa tctacaaatg tggactttgc 3000
tgtcaataca gagggaactt attctgagcc tcgccccatt ggtactcgtt acctcacccg 3060
taatctgtaa ttgctggtta atcaataaac cgtttgattc gtttcagttg aactttggtc 3120
tctgcgaagg gcgaattcgt ttaaacctgc aggactagtc cctttagtga gg 3172
<210>176
<211>3160
<212>DNA
<213>新AAV血清型,克隆hu145.1
<400>176
acccttcact aaagggacta gtcctgcagg tttaaacgaa ttcgcccttg ctgcgtcaac 60
tggaccaatg agaactttcc cttcaacgac tgtgtcgaca agatggtgat ttggtgggag 120
gaggggaaga tgaccgccaa ggtcgtggag tcggccaaag ccattctcgg aggaagcaag 180
gtgcgcgtgg accagaaatg caagtcctcg gcccagatag atccgactcc cgtgatcgtc 240
acctccaaca ccaacatgtg cgccgtgatt gacgggaact caacgacctt cgaacaccag 300
cagccgttgc aagaccggat gttcaaattt gaactcaccc gccgtctgga tcatgacttt 360
gggaaggtca ccaagcggga agtcaaagac tttttccggt gggcaaagga tcacgtggtt 420
gaggtggagc atgaattcta cgtcaaaaag ggtggagcca agaaaagacc cgcccctagt 480
gacgcagata taagtgagcc caaacgggtg cgcgagtcag ttgcgcagcc atcgacgtca 540
gacgcggaag cttcgatcaa ctacgcggac aggtaccaaa acaaatgttc tcgtcacgtg 600
ggcatgaatc tgatgctgtt tccctgcaga caatgcgaga gaatgaatca aaattcaaat 660
atctgcttca ctcacggaca gaaagactgt ttagagtgct ttcccgtgtc agaatctcaa 720
cctgtttctg tcgtcaaaaa ggcgtatcag aaactgtgct acattcatca tatcatggga 780
aaggtgccag acgcttgcac tgcctgcgat ctggtcaatg tggatttgga tgactgcatc 840
tctgaacaat aaatgattta aatcaggtat ggctgccgat ggttatcttc cagattggct 900
cgaggacact ctctctgaag gaataagaca gtggtggaag ctcaaacctg gcccaccacc 960
accaaagccc gcagagcggc ataaggacga cagcaggggt cttgtgcttc ctgggtacaa 1020
gtacctcgga cccttcaacg gactcgacaa gggagagccg gtcaacgagg cagacgccgc 1080
ggccctcgag cacgacaagg cctacgaccg gcagctcgac agcggagaca acccgtacct 1140
caagtacaac cacgccgacg cggagtttca ggagcgtctt aaagaagata cgtcttttgg 1200
gggcaacctc ggacgagcag tcttccaggc gaaaaagagg gttcttgaac ctctgggcct 1260
ggttgaggaa cctgttaaga cggctccggg aaaaaagagg ccggtagagc actctcctgc 1320
ggagccagac tcctcctcgg gaaccggaaa agcgggccag cagcctgcaa gaaaaagact 1380
gaatttcggt cagactggag acgcagactc cgtacctgac ccccagcctc tcagacagcc 1440
accagcagcc cccacaagtt tgggatctac tacaatggct acaggcagtg gcgcaccaat 1500
ggcagacaat aacgagggtg ccgatggagt gggtaattcc tcaggaaatt ggcattgcga 1560
ttcccaatgg ctgggcgaca gagtcatcac caccagcacc cgaacctggg ccctgcccac 1620
ctacaacaac cacctttaca agcaaatctc cagccaatca ggagcctcaa acgacaacca 1680
ctactttggc tacagcaccc cctgggggta ttttgacttc aacagattcc actgccactt 1740
ttcaccacgt gactggcaaa gactcatcaa caacaactgg ggattccgac ccaagagact 1800
caacttcaag ctctttaaca ttcaagtcaa agaggtcacg cagaatgacg gtacgacgac 1860
gattgccaat aaccttacca gcacggttca ggtgtttact gactcggagt accagctccc 1920
gtacgtcctc ggctcggcgc atcaaggatg cctcccgccg tttccagcgg acgtcttcat 1980
ggtcccacag tatggatacc tcaccctgaa caacgggagt caggcggtag gacgctcttc 2040
cttttactgc ctggagtact ttccttctca gatgctgcgt actggaaaca actttcagtt 2100
cagctacact tttgaagacg tgcctttcca cagcagctac gctcacagcc agagtttgga 2160
tcggctgatg aatcctctga tcgaccagta cctgtattat ctaaacagaa cacaaacagc 2220
tagtggaact cagcagtctc ggctactgtt tagccaagct ggacccacaa gcatgtctct 2280
tcaagctaaa aactggctgc ctggaccgtg ttatcgccag cagcgtttgt caaagcaggc 2340
aaacgacaac aacaacagca actttccctg gactggagct accaagtact acctcaatgg 2400
cagagactct ttggtgaacc cgggcccggc catggccagc cacaaggacg atgaagaaaa 2460
gtttttcccc atgcatggaa ccctaatatt tggtaaagaa ggaacaaatg ctaccaacgc 2520
ggaattggaa aatgtcatga ttacagatga agaggaaatc aggaccacca atcccgtggc 2580
tacagagcag tacggatatg tgtcaaataa tttgcaaaac tcaaatactg ctgcaagtac 2640
tgaaactgtg aatcaccaag gagcattacc tggtatggtg tggcaggatc gagacgtgta 2700
cctgcaggga cccatttggg ccaagattcc tcacaccgat ggacactttc atccttctcc 2760
actgatggga ggttttggac tcaaacaccc gcctcctcag attatgatca aaaacactcc 2820
cgttccagcc aatcctccca caaacttcag ttctgccaag tttgcttcct tcatcacaca 2880
gtattccacg ggacaggtca gcgtggagat cgagtgggag ctgcagaagg agaacagcaa 2940
acgctggaat cccgaaattc agtacacttc caactacaac aaatctgtta atgtggactt 3000
tactgtggac actaatggtg tgtattcaga gcctcgcccc attggcacca gatacctgac 3060
tcgtaatctg taattgcttg ttaatcaata aaccgtttaa ttcgtttcag ttgaactttg 3120
gtctctgcga agggcgaatt cgcggccgct aaatcaatcg 3160
<210>177
<211>3157
<212>DNA
<213>新AAV血清型,克隆hu145.5
<400>177
ctcactaagg gactagtcct gcaggtttaa acgaattcgc ccttgctgcg tcaactggac 60
caatgagaac tttcccttca acgactgtgt cgacaagatg gtgatttggt gggaggaggg 120
gaagatgacc gccaaggtcg tggagtcggc caaagccact ctcggaggaa gcaaggtgcg 180
cgtggaccag aaacgcaagt cctcggccca gatagatccg actcccgtga tcgtcacctc 240
caacaccaac atgtgcgccg tgattgacgg gaactcaacg accttcgaac accagcagcc 300
gttgcaagac cgaatgttca aatttgaact cacccgccgt ctggatcatg acttcgggaa 360
ggtcaccaag caggaagtca aagacttttt ccggtgggca aaggatcacg tggttgaggt 420
ggagcatgaa ttctacgtca aaaagggtgg agccaagaaa agacccgccc ctagtgacgc 480
agatataagt gagcccaaac gggtgcgcga gtcagttgcg cagccatcga cgtcagacgc 540
ggaagcttcg atcaactacg cggacaggta ccaaaacaaa tgttctcgtc acgtgggcat 600
gaatctgatg ctgtttccct gcagacaatg cgagagaatg aatcaaaatt caaatatctg 660
cttcactcac ggacagaaag actgtttaga gtgctttccc gtgtcagaat ctcaacctgt 720
ttctgtcgtc aaaaaggcgt atcagaaact gtgctacatt catcatatca tgggaaaggt 780
gccagacgct tgcactgcct gcgatctggt caatgtggat ttggatgact gcatctctga 840
acaataaatg atttaaatca ggtatggctg ccgatggtta tcttccagat tggctcgagg 900
acactctctc tgaaggaata agacagtggt ggaagctcaa acctggccca ccaccaccaa 960
agcccgcaga gcggcataag gacgacagca ggggtcttgt gcttcctggg tacaagtacc 1020
tcggaccctt caacggactc gacaagggag agccggtcaa cgaggcagac gccgcggctc 1080
tcgagcacga caaggcctac gaccggcagc tcgacagcgg agacaacccg tacctcaagt 1140
acaaccacgc cgacgcggag tttcaggagc gtcttaaaga agatacgtct tttgggggca 1200
acctcggacg agcagtcttc caggcgaaaa agagggttct tgaacctctg ggcctggttg 1260
aggaacctgt taagacggct ccgggaaaaa agaggccggt agagcactct cctgcggagc 1320
cagactcctc ctcgggaacc ggaaaagcgg gccagcagcc tgcaagaaaa agactgaatt 1380
tcggtcagac tggagacgca gactccgtac ctgaccccca gcctctcgga cagccaccag 1440
cagcccccac aagtttggga tctactacaa tggctacagg cagtggcgca ccaatggcag 1500
acaataacga gggtgccgat ggagtgggta attcctcagg aaattggcat tgcgattccc 1560
aatggctggg cgacagagtc atcaccacca gcacccgaac ctgggccctg cccacctaca 1620
acaaccacct ttacaagcaa atctccagcc aatcaggagc ctcaaacgac aaccactact 1680
ttggctacag caccccctgg gggtattttg acttcaacag attccactgc cgcttttcac 1740
cacgtgactg gcaaagactc atcaacaaca actggggatt ccgacccaag agactcaact 1800
tcaagctctt taacattcaa gtcaaagagg tcacgcagaa tgacggtacg acgacgattg 1860
ccaataacct taccagcacg gttcaggtgt ttactgactc ggagtaccag ctcccgtacg 1920
tcctcggctc ggcgcatcaa ggatgcctcc cgccgtttcc agcggacgtc ttcatggtcc 1980
cacagtatgg atacctcacc ctgaacaacg ggagtcaggc ggtaggacgc tcttcctttt 2040
actgcctgga gtactttcct tctcagatgc tgcgtactgg aaacaacttt cagttcagct 2100
acacttttga agacgtgcct ttccacagca gctacgctca cagccagggt ttggatcggc 2160
tgatgaatcc tctgatcgac cagtacctgt attatctaaa cagaacacaa acagctagtg 2220
gaactcagca gtctcggcta ctgtttagcc aagctggacc cacaagcatg tctcttcaag 2280
ctaaaaactg gctgcctgga ccgtgttatc gccagcagcg tttgtcaaag caggcaaacg 2340
acaacaacaa cagcaacttt ccctggactg gagctaccaa gtaccacctc aatggcggag 2400
actctttggt gaacccgggc ccggccatgg ccagccacaa ggacgatgaa gaaaagtttt 2460
tccccatgca tggaacccta atatttggta aagaaggaac aaatgctacc aacgcggaat 2520
tggaaaatgt catgattaca gatgaagagg aaatcaggac caccaatccc gtggctacag 2580
agcagtacgg atatgtgtca aataatttgc aaaactcaaa tactgctgca agtactgaaa 2640
ctgtgaatca ccaaggagca ttacctggta tggtgtggca ggatcgagac gtgtacctgc 2700
ggggacccat ttgggccaag attcctcacg ccgatggaca ctttcatcct tctccactga 2760
tgggaggttt tggactcaaa cacccgcctc ctcagattat gatcaaaaac actcccgttc 2820
cagccaatcc tcccacaaac ttcagttctg ccaagtttgc ttccttcatc acacagtatt 2880
ccacgggaca ggtcagcgtg gagatcgagt gggagctgca gaaggagaac agcaaacgct 2940
ggaatcccga aattcagtac acttccaact acaacaaatc tgttaatgtg gactttactg 3000
tggacactaa tggtgtgtat tcagagcctc gccccattgg caccagatac ctgactcgta 3060
atctgtaatt gcttgttaat caataaaccg tttaattcgt ttcagttgaa ctttggtctc 3120
tgcgaagggc gaattcgcgg ccgctaaatt caattcg 3157
<210>178
<211>3163
<212>DNA
<213>新AAV血清型,克隆hu145.6
<400>178
accctcacta aagggactag tcctgcaggt ttaaacgaat tcgcccttgc tgcgtcaact 60
ggagcaatga gaactttccc ttcaacgact gtgtcgacaa gatggtgatt tggtgggagg 120
aggggaagat gaccgccaag gtcgtggagt cggccaaagc cattctcgga ggaagcaagg 180
tgcgcgtgga ccagaaatgc aagtcctcgg cccagataga tccgactccc gtgatcgtca 240
cctccaacac caacatgtgc gccgtgattg acgggaactc aacgaccttc gaacaccagc 300
agccgttgca agaccggatg ttcaaatttg aactcacccg ccgtctggat catgactttg 360
ggaaggtcac caagcaggaa gtcaaagact ttttccggtg ggcaaaggat cacgtggttg 420
aggtggagca tggattctac gtcaaaaagg gtggagccaa gaaaagaccc gcccctagtg 480
acgcagatat aagtgagccc aaacgggtgc gcgagtcagt tgcgcagcca tcgacgtcgg 540
acgcggaagc ttcgatcaac tacgcggaca ggtaccaaaa caaatgttct cgtcacgtgg 600
gcatgaatct gatgctgttt ccctgcagac aatgcgagag aatgaatcaa aattcaaata 660
tctgcttcac tcacggacag aaagactgtt tagagtgctt tcccgtgtca gaatctcaac 720
ctgtttctgt cgtcaaaaag gcgtatcaga aactgcgcta cattcatcat atcatgggaa 780
aggtgccaga cgcttgcact gcctgcgatc tggtcaatgt ggatttggat gactgcatct 840
ctgaacaata aatgatttaa atcaggtatg gctgccgatg gttatcttcc agattggctc 900
gaggacactc tctctgaagg aataagacag tggtggaagc tcaaacctgg cccaccacca 960
ccaaagcccg cagagcggca taaggacgac agcaggggtc ttgtgcttcc tgggtacaag 1020
tacctcggac ccttcaacgg actcgacaag ggagagccgg tcaacgaggc agacgccgcg 1080
gccctcgagc acgacaaggc ctacgaccgg cagctcgaca gcggagacaa cccgtacctc 1140
aagtacaacc acgccgacgc ggagtttcag gagcgtctta aagaagatac gtcttttggg 1200
ggcaacctcg gacgagcagt cttccaggcg aaaaagaggg ttcttgaacc tctgggcctg 1260
gttgaggaac ctgttaagac ggctccggga aaaaagaggc cggtagagca ctctcctgcg 1320
gagccagact cctcctcggg aaccggaaaa gcgggccagc agcctgcaag aaaaagactg 1380
aatttcggtc agactggaga cgcagactcc gtacctgacc cccagcctct cggacagcca 1440
ccagcagccc ccacaagttt gggatctact acaatggcta caggcagtgg cgcaccaatg 1500
gcagacaata acgagggtgc cgatggagtg ggtaattcct caggaaattg gcattgcgat 1560
tcccaatggc tgggcgacag agtcatcacc accagcaccc gaacctgggc cctgcccacc 1620
tacaacaacc acctttacaa gcaaatctcc agccaatcag gagcctcaaa cgacaaccac 1680
tactttggct acagcacccc ctgggggtat tttgacttca acagattcca ctgccacttt 1740
tcaccacgtg actggcaaag actcatcaac aacaactggg gattccgacc caagagactc 1800
aacttcaagc tctttaacat tcaagtcaaa gaggtcacgc agaatgacgg tacgacgacg 1860
attgccaata accttaccag cacggttcag gtgtttactg actcggagta ccagctcccg 1920
tacgtcctcg gctcggcgca tcaaggatgc ctcccgccgt ttccagcgga cgtcttcatg 1980
gtcccacagt atggatacct caccctgaac aacgggagtc aggcggtagg acgctcttcc 2040
ttttactgcc tggagtgctt tccttctcag atgctgcgta ctggaaacaa ctttcagttc 2100
agctacactt ttgaagacgt gcctttccac agcagctacg ctcacagcca gagtttggat 2160
cggctgatga atcctctgat cgaccagtac ctgtattatc taaacagaac acaaacagct 2220
agtggaactc agcagtctcg gctactgttt agccaagctg gacccacaag catgtctctt 2280
caagctaaaa actggctgcc tggaccgtgt tatcgccagc agcgtttgtc aaagcaggca 2340
aacgacaaca acaacagcaa ctttccctgg actggagcta ccaagtacca cctcaatggc 2400
agagactctt tggtgaaccc gggcccggcc atggccagcc acaaggacga tgaagaaaag 2460
tttttcccca tgcatggaac cctaatattt ggtaaagaag gaacaaatgc taccaacgcg 2520
gaattggaaa atgtcatgat tacagatgaa gaggaaatca ggaccaccaa tcccgtggct 2580
acagagcagt acggatatgt gtcaaataat ttgcaaaact caaatactgc tgcaagtact 2640
gaaactgtga atcaccaagg agcattacct ggtatggtgt ggcaggatcg agacgtgtac 2700
ctgcagggac ccatttgggc caagattcct cacaccgatg gacactttca tccttctcca 2760
ctgatgggag gttttggtct caaacacccg cctcctcaga ttatgatcaa aaacactccc 2820
gttccagcca atcctcccac aaacttcagt tctgccaagt ttgcttcctt catcacacag 2880
tattccacgg gacaggtcag cgtggagatc gagtgggagc tgcagaagga gaacagcaaa 2940
cgctggaatc ccgaaattca gtacacttcc aactacaaca aatctgttaa tgtggacttt 3000
actgtggaca ctaatggtgt gtattcagag cctcgcccca ttggcaccag atacctgact 3060
cgtaatctgt aattgcttgt taatcaataa accgtttaat tcgtttcagt tgaactttgg 3120
tctctgcgaa gggcgaattc gcggccgcta aattcaattc gcc 3163
<210>179
<211>3161
<212>DNA
<213>新AAV血清型,克隆hu156.1
<400>179
cgaattgatt tagcggccgc gaattcgccc ttcgcagaga ccaaagttca actgaaacga 60
attaaacggt ttattgatta acaagcaatt acagattacg agtcaggtat ctggtgccaa 120
tggggcgagg ctctgaatac acaccattag tgtccacagt aaagtccaca ttaacagatt 180
tgttgtagtt ggaagtgtac tggatctcgg gattccagcg tttgctgttc tccttctgta 240
gctcccactc gatctccacg ctgacccgcc ccgtggaata ctgtgtgatg aaggaagcaa 300
actttgccgc actgaaggtg gtcgaaggat tcgcaggtac cggggtgttt ttgatgagaa 360
tctgtggagg agggtgttta agtccgaatc cgcccatgag gggagagggg tgaaaatgtc 420
cgtccgtgtg cggaatcttt gcccagatag gcccctgcag gtacacgtct ctgtcctgcc 480
agaccatgcc tggaagaacg ccttgtgtgt tgacatctgc agtagatgct tgtgtgttgc 540
cgctctggag gttggtagat acagaaccat actgctccgt ggccacggga ttggtggttc 600
tgatttcctc ttcgtctgta atcatgacct tttcaatgtc cacatttgtt ttctctgatc 660
cttgttttcc aaagatgaga accccgctct gaggaaaaaa cttttcttca tcgtccttgt 720
ggctggccat tgccgggccc ggattcacca gagagtctct gccattgagg tggtacttgg 780
tagctccaat ccacgagtat tcactgttgt tgttgtccgc agatgtcttt gatactcgct 840
gctggcggta acagggtcca ggaagccagt tcctagactg atcccgaatg tcactcgctc 900
cggcctgaga aaactgaagc ctggactgcg tggtggttcc gcttggagtg tttgttctgc 960
tcaagtaata caggtactgg tcgatgagag gattcatgag acggtccaaa ctctggctgt 1020
gagcgtagct gctgtggaaa ggaacatcct caaaggtgta gctgaaggta aagttgtttc 1080
cggtacgcag catctgagaa gggaagtact ccaggcagta aaatgaagag cgtcctactg 1140
cctgactccc gttgttcagg gtgaggtatc catactgtgg caccatgaag acgtctgctg 1200
ggaacggcgg gaggcatcct tgatgcgccg agccgaggac gtacgggagc tggtactccg 1260
agtcagtaaa cacctgaacc gtgctggtaa ggttattggc aatcgtcgtc gtaccatcat 1320
tctgcgtgac ctctctgact tgaatgttaa agagcttgaa gttgagtctc ttgggccgga 1380
atccccagtt gttgttgatg agtctttgcc agtcacgtgg tgaaaagtgg cagtggaatc 1440
tgttgaagtc aaaatacccc caaggggtgc tgtagccaaa gtagtggttg tcgttgctgg 1500
ctcctgattg gctggaaatc tgcttgtaca gatggttgtt gtaggtgggc agagcccagg 1560
ttcgggtgct ggtggtgatg actctgtcgc ccatccatgt ggaatcgcaa tgccaatttc 1620
ccgaggaatt acccactccg tcggcgccat cgttattgtc tgccattggt gcgccactgc 1680
ctgtagccat cgtattagtt cccagaccag agggggctgc tggtggctgt ccgagaggct 1740
gggggtcagg tacggagtct gcgtctccag tctgaccgaa attcaatctc tttcttgcag 1800
gctggttgcc cgcttttccg gttcccgagg aggagtctgg ctccacagga gagtgctcta 1860
ccggcctctt ttttcccgga gccgtcttaa caggctcctc aaccaggccc agaggttcaa 1920
gaaccctctt ttttgcctgg aagactgctc gtccgaggtt gcccccaaaa gacgtatctt 1980
ctttaaggcg ctcctgaaac tccgcgtcgg cgtggtcgta cttgaggtac gggttgtctc 2040
cgctgtcgag ctgccggtcg taggccttgt cgtgctcgag ggccgcggcg tctgcctcgt 2100
tgaccggctc tcccttgtcg agtccgttga agggtccgag gtacttgtac ccaggaagca 2160
caagacccct gctgtcgtcc ttatgccgct ctgcgggctt tggtggtggt gggccaggtt 2220
tgagcttcca ccactgtctt attccttcag agagagtgtc ctcgagccaa tctggaagat 2280
aaccatcggc agccatacct gatttaaatc atttattgtt cagagatgca gtcatccaaa 2340
tccacattga ccagatcgca ggcagtgcaa gcgtctggca cctttcccat gatatgatga 2400
atgtagcaaa gtttctgata cgcctttttg acgacagaaa cgggttgaga ttctgacacg 2460
ggaaagcact ctaaacagtc tttctgtccg tgagtgaagc agatatttga attctgattc 2520
attctctcgc attgtctgca gggaaacagc atcagattca tgcccacgtg acgagaacat 2580
ttgttttggt acctgtccgc gtagttgatc gaagcttccg cgtctgacgt cgatggctgc 2640
gcaactgact cgcgcgcccg tttgggctca cttatatctg cgtcactggg ggcgggtctt 2700
ttcttggctc cacccttttt gacgtagaat tcatgctcca cctcaaccac gtgatccttt 2760
gcccaccgga aaaagtcttt cacttcctgc ttggtgacct tcccaaagtc atgatccaga 2820
cggcgggtta gttcaaattt gaacatccgg tcttgcaacg gctgctggtg ttcgaaggtc 2880
gttgagttcc cgtcaatcac ggcgcacatg ttggtgttgg aggtggcgat cacgggagtc 2940
gggtctatct gggccgagga cttgcacttt tggtccacgc gcaccttgct tcctccgaga 3000
atggcttcgg ccgattccac gaccttggcg gtcatctttc cctcctccca ccagatcacc 3060
atcttgtcga cacagtcgtt gaagggaaag ttctcattgg tccagttgac gcagcaaggg 3120
cgaattcgtt taaacctgca ggaactagtc ccttagtgag g 3161
<210>180
<211>4721
<212>DNA
<213>腺伴随病毒血清型7
<400>180
ttggccactc cctctatgcg cgctcgctcg ctcggtgggg cctgcggacc aaaggtccgc 60
agacggcaga gctctgctct gccggcccca ccgagcgagc gagcgcgcat agagggagtg 120
gccaactcca tcactagggg taccgcgaag cgcctcccac gctgccgcgt cagcgctgac 180
gtaaatcacg tcatagggga gtggtcctgt attagctgtc acgtgagtgc ttttgcgaca 240
ttttgcgaca ccacgtggcc atttgaggta tatatggccg agtgagcgag caggatctcc 300
attttgaccg cgaaatttga acgagcagca gccatgccgg gtttctacga gatcgtgatc 360
aaggtgccga gcgacctgga cgagcacctg ccgggcattt ctgactcgtt tgtgaactgg 420
gtggccgaga aggaatggga gctgcccccg gattctgaca tggatctgaa tctgatcgag 480
caggcacccc tgaccgtggc cgagaagctg cagcgcgact tcctggtcca atggcgccgc 540
gtgagtaagg ccccggaggc cctgttcttt gttcagttcg agaagggcga gagctacttc 600
caccttcacg ttctggtgga gaccacgggg gtcaagtcca tggtgctagg ccgcttcctg 660
agtcagattc gggagaagct ggtccagacc atctaccgcg gggtcgagcc cacgctgccc 720
aactggttcg cggtgaccaa gacgcgtaat ggcgccggcg gggggaacaa ggtggtggac 780
gagtgctaca tccccaacta cctcctgccc aagacccagc ccgagctgca gtgggcgtgg 840
actaacatgg aggagtatat aagcgcgtgt ttgaacctgg ccgaacgcaa acggctcgtg 900
gcgcagcacc tgacccacgt cagccagacg caggagcaga acaaggagaa tctgaacccc 960
aattctgacg cgcccgtgat caggtcaaaa acctccgcgc gctacatgga gctggtcggg 1020
tggctggtgg accggggcat cacctccgag aagcagtgga tccaggagga ccaggcctcg 1080
tacatctcct tcaacgccgc ctccaactcg cggtcccaga tcaaggccgc gctggacaat 1140
gccggcaaga tcatggcgct gaccaaatcc gcgcccgact acctggtggg gccctcgctg 1200
cccgcggaca ttaaaaccaa ccgcatctac cgcatcctgg agctgaacgg gtacgatcct 1260
gcctacgccg gctccgtctt tctcggctgg gcccagaaaa agttcgggaa gcgcaacacc 1320
atctggctgt ttgggcccgc caccaccggc aagaccaaca ttgcggaagc catcgcccac 1380
gccgtgccct tctacggctg cgtcaactgg accaatgaga actttccctt caacgattgc 1440
gtcgacaaga tggtgatctg gtgggaggag ggcaagatga cggccaaggt cgtggagtcc 1500
gccaaggcca ttctcggcgg cagcaaggtg cgcgtggacc aaaagtgcaa gtcgtccgcc 1560
cagatcgacc ccacccccgt gatcgtcacc tccaacacca acatgtgcgc cgtgattgac 1620
gggaacagca ccaccttcga gcaccagcag ccgttgcagg accggatgtt caaatttgaa 1680
ctcacccgcc gtctggagca cgactttggc aaggtgacga agcaggaagt caaagagttc 1740
ttccgctggg ccagtgatca cgtgaccgag gtggcgcatg agttctacgt cagaaagggc 1800
ggagccagca aaagacccgc ccccgatgac gcggatataa gcgagcccaa gcgggcctgc 1860
ccctcagtcg cggatccatc gacgtcagac gcggaaggag ctccggtgga ctttgccgac 1920
aggtaccaaa acaaatgttc tcgtcacgcg ggcatgattc agatgctgtt tccctgcaaa 1980
acgtgcgaga gaatgaatca gaatttcaac atttgcttca cacacggggt cagagactgt 2040
ttagagtgtt tccccggcgt gtcagaatct caaccggtcg tcagaaaaaa gacgtatcgg 2100
aaactctgcg cgattcatca tctgctgggg cgggcgcccg agattgcttg ctcggcctgc 2160
gacctggtca acgtggacct ggacgactgc gtttctgagc aataaatgac ttaaaccagg 2220
tatggctgcc gatggttatc ttccagattg gctcgaggac aacctctctg agggcattcg 2280
cgagtggtgg gacctgaaac ctggagcccc gaaacccaaa gccaaccagc aaaagcagga 2340
caacggccgg ggtctggtgc ttcctggcta caagtacctc ggacccttca acggactcga 2400
caagggggag cccgtcaacg cggcggacgc agcggccctc gagcacgaca aggcctacga 2460
ccagcagctc aaagcgggtg acaatccgta cctgcggtat aaccacgccg acgccgagtt 2520
tcaggagcgt ctgcaagaag atacgtcatt tgggggcaac ctcgggcgag cagtcttcca 2580
ggccaagaag cgggttctcg aacctctcgg tctggttgag gaaggcgcta agacggctcc 2640
tgcaaagaag agaccggtag agccgtcacc tcagcgttcc cccgactcct ccacgggcat 2700
cggcaagaaa ggccagcagc ccgccagaaa gagactcaat ttcggtcaga ctggcgactc 2760
agagtcagtc cccgaccctc aacctctcgg agaacctcca gcagcgccct ctagtgtggg 2820
atctggtaca gtggctgcag gcggtggcgc accaatggca gacaataacg aaggtgccga 2880
cggagtgggt aatgcctcag gaaattggca ttgcgattcc acatggctgg gcgacagagt 2940
cattaccacc agcacccgaa cctgggccct gcccacctac aacaaccacc tctacaagca 3000
aatctccagt gaaactgcag gtagtaccaa cgacaacacc tacttcggct acagcacccc 3060
ctgggggtat tttgacttta acagattcca ctgccacttc tcaccacgtg actggcagcg 3120
actcatcaac aacaactggg gattccggcc caagaagctg cggttcaagc tcttcaacat 3180
ccaggtcaag gaggtcacga cgaatgacgg cgttacgacc atcgctaata accttaccag 3240
cacgattcag gtattctcgg actcggaata ccagctgccg tacgtcctcg gctctgcgca 3300
ccagggctgc ctgcctccgt tcccggcgga cgtcttcatg attcctcagt acggctacct 3360
gactctcaac aatggcagtc agtctgtggg acgttcctcc ttctactgcc tggagtactt 3420
cccctctcag atgctgagaa cgggcaacaa ctttgagttc agctacagct tcgaggacgt 3480
gcctttccac agcagctacg cacacagcca gagcctggac cggctgatga atcccctcat 3540
cgaccagtac ttgtactacc tggccagaac acagagtaac ccaggaggca cagctggcaa 3600
tcgggaactg cagttttacc agggcgggcc ttcaactatg gccgaacaag ccaagaattg 3660
gttacctgga ccttgcttcc ggcaacaaag agtctccaaa acgctggatc aaaacaacaa 3720
cagcaacttt gcttggactg gtgccaccaa atatcacctg aacggcagaa actcgttggt 3780
taatcccggc gtcgccatgg caactcacaa ggacgacgag gaccgctttt tcccatccag 3840
cggagtcctg atttttggaa aaactggagc aactaacaaa actacattgg aaaatgtgtt 3900
aatgacaaat gaagaagaaa ttcgtcctac taatcctgta gccacggaag aatacgggat 3960
agtcagcagc aacttacaag cggctaatac tgcagcccag acacaagttg tcaacaacca 4020
gggagcctta cctggcatgg tctggcagaa ccgggacgtg tacctgcagg gtcccatctg 4080
ggccaagatt cctcacacgg atggcaactt tcacccgtct cctttgatgg gcggctttgg 4140
acttaaacat ccgcctcctc agatcctgat caagaacact cccgttcccg ctaatcctcc 4200
ggaggtgttt actcctgcca agtttgcttc gttcatcaca cagtacagca ccggacaagt 4260
cagcgtggaa atcgagtggg agctgcagaa ggaaaacagc aagcgctgga acccggagat 4320
tcagtacacc tccaactttg aaaagcagac tggtgtggac tttgccgttg acagccaggg 4380
tgtttactct gagcctcgcc ctattggcac tcgttacctc acccgtaatc tgtaattgca 4440
tgttaatcaa taaaccggtt gattcgtttc agttgaactt tggtctcctg tgcttcttat 4500
cttatcggtt tccatagcaa ctggttacac attaactgct tgggtgcgct tcacgataag 4560
aacactgacg tcaccgcggt acccctagtg atggagttgg ccactccctc tatgcgcgct 4620
cgctcgctcg gtggggcctg cggaccaaag gtccgcagac ggcagagctc tgctctgccg 4680
gccccaccga gcgagcgagc gcgcatagag ggagtggcca a 4721
<210>181
<211>737
<212>PRT
<213>腺伴随病毒血清型7的衣壳蛋白
<400>181
Met Ala Ala Asp Gly Tyr Leu Pro Asp Trp Leu Glu Asp Asn Leu Ser
1 5 10 15
Glu Gly Ile Arg Glu Trp Trp Asp Leu Lys Pro Gly Ala Pro Lys Pro
20 25 30
Lys Ala Asn Gln Gln Lys Gln Asp Asn Gly Arg Gly Leu Val Leu Pro
35 40 45
Gly Tyr Lys Tyr Leu Gly Pro Phe Asn Gly Leu Asp Lys Gly Glu Pro
50 55 60
Val Asn Ala Ala Asp Ala Ala Ala Leu Glu His Asp Lys Ala Tyr Asp
65 70 75 80
Gln Gln Leu Lys Ala Gly Asp Asn Pro Tyr Leu Arg Tyr Asn His Ala
85 90 95
Asp Ala Glu Phe Gln Glu Arg Leu Gln Glu Asp Thr Ser Phe Gly Gly
100 105 110
Asn Leu Gly Arg Ala Val Phe Gln Ala Lys Lys Arg Val Leu Glu Pro
115 120 125
Leu Gly Leu Val Glu Glu Gly Ala Lys Thr Ala Pro Ala Lys Lys Arg
130 135 140
Pro Val Glu Pro Ser Pro Gln Arg Ser Pro Asp Ser Ser Thr Gly Ile
145 150 155 160
Gly Lys Lys Gly Gln Gln Pro Ala Arg Lys Arg Leu Asn Phe Gly Gln
165 170 175
Thr Gly Asp Ser Glu Ser Val Pro Asp Pro Gln Pro Leu Gly Glu Pro
180 185 190
Pro Ala Ala Pro Ser Ser Val Gly Ser Gly Thr Val Ala Ala Gly Gly
195 200 205
Gly Ala Pro Met Ala Asp Asn Asn Glu Gly Ala Asp Gly Val Gly Asn
210 215 220
Ala Ser Gly Asn Trp His Cys Asp Ser Thr Trp Leu Gly Asp Arg Val
225 230 235 240
Ile Thr Thr Ser Thr Arg Thr Trp Ala Leu Pro Thr Tyr Asn Asn His
245 250 255
Leu Tyr Lys Gln Ile Ser Ser Glu Thr Ala Gly Ser Thr Asn Asp Asn
260 265 270
Thr Tyr Phe Gly Tyr Ser Thr Pro Trp Gly Tyr Phe Asp Phe Asn Arg
275 280 285
Phe His Cys His Phe Ser Pro Arg Asp Trp Gln Arg Leu Ile Asn Asn
290 295 300
Asn Trp Gly Phe Arg Pro Lys Lys Leu Arg Phe Lys Leu Phe Asn Ile
305 310 315 320
Gln Val Lys Glu Val Thr Thr Asn Asp Gly Val Thr Thr Ile Ala Asn
325 330 335
Asn Leu Thr Ser Thr Ile Gln Val Phe Ser Asp Ser Glu Tyr Gln Leu
340 345 350
Pro Tyr Val Leu Gly Ser Ala His Gln Gly Cys Leu Pro Pro Phe Pro
355 360 365
Ala Asp Val Phe Met Ile Pro Gln Tyr Gly Tyr Leu Thr Leu Asn Asn
370 375 380
Gly Ser Gln Ser Val Gly Arg Ser Ser Phe Tyr Cys Leu Glu Tyr Phe
385 390 395 400
Pro Ser Gln Met Leu Arg Thr Gly Asn Asn Phe Glu Phe Ser Tyr Ser
405 410 415
Phe Glu Asp Val Pro Phe His Ser Ser Tyr Ala His Ser Gln Ser Leu
420 425 430
Asp Arg Leu Met Asn Pro Leu Ile Asp Gln Tyr Leu Tyr Tyr Leu Ala
435 440 445
Arg Thr Gln Ser Asn Pro Gly Gly Thr Ala Gly Asn Arg Glu Leu Gln
450 455 460
Phe Tyr Gln Gly Gly Pro Ser Thr Met Ala Glu Gln Ala Lys Asn Trp
465 470 475 480
Leu Pro Gly Pro Cys Phe Arg Gln Gln Arg Val Ser Lys Thr Leu Asp
485 490 495
Gln Asn Asn Asn Ser Asn Phe Ala Trp Thr Gly Ala Thr Lys Tyr His
500 505 510
Leu Asn Gly Arg Asn Ser Leu Val Asn Pro Gly Val Ala Met Ala Thr
515 520 525
His Lys Asp Asp Glu Asp Arg Phe Phe Pro Ser Ser Gly Val Leu Ile
530 535 540
Phe Gly Lys Thr Gly Ala Thr Asn Lys Thr Thr Leu Glu Asn Val Leu
545 550 555 560
Met Thr Asn Glu Glu Glu Ile Arg Pro Thr Asn Pro Val Ala Thr Glu
565 570 575
Glu Tyr Gly Ile Val Ser Ser Asn Leu Gln Ala Ala Asn Thr Ala Ala
580 585 590
Gln Thr Gln Val Val Asn Asn Gln Gly Ala Leu Pro Gly Met Val Trp
595 600 605
Gln Asn Arg Asp Val Tyr Leu Gln Gly Pro Ile Trp Ala Lys Ile Pro
610 615 620
His Thr Asp Gly Asn Phe His Pro Ser Pro Leu Met Gly Gly Phe Gly
625 630 635 640
Leu Lys His Pro Pro Pro Gln Ile Leu Ile Lys Asn Thr Pro Val Pro
645 650 655
Ala Asn Pro Pro Glu Val Phe Thr Pro Ala Lys Phe Ala Ser Phe Ile
660 665 670
Thr Gln Tyr Ser Thr Gly Gln Val Ser Val Glu Ile Glu Trp Glu Leu
675 680 685
Gln Lys Glu Asn Ser Lys Arg Trp Asn Pro Glu Ile Gln Tyr Thr Ser
690 695 700
Asn Phe Glu Lys Gln Thr Gly Val Asp Phe Ala Val Asp Ser Gln Gly
705 710 715 720
Val Tyr Ser Glu Pro Arg Pro Ile Gly Thr Arg Tyr Leu Thr Arg Asn
725 730 735
Leu
<210>182
<211>4393
<212>DNA
<213>腺伴随病毒血清型8
<400>182
cagagaggga gtggccaact ccatcactag gggtagcgcg aagcgcctcc cacgctgccg 60
cgtcagcgct gacgtaaatt acgtcatagg ggagtggtcc tgtattagct gtcacgtgag 120
tgcttttgcg gcattttgcg acaccacgtg gccatttgag gtatatatgg ccgagtgagc 180
gagcaggatc tccattttga ccgcgaaatt tgaacgagca gcagccatgc cgggcttcta 240
cgagatcgtg atcaaggtgc cgagcgacct ggacgagcac ctgccgggca tttctgactc 300
gtttgtgaac tgggtggccg agaaggaatg ggagctgccc ccggattctg acatggatcg 360
gaatctgatc gagcaggcac ccctgaccgt ggccgagaag ctgcagcgcg acttcctggt 420
ccaatggcgc cgcgtgagta aggccccgga ggccctcttc tttgttcagt tcgagaaggg 480
cgagagctac tttcacctgc acgttctggt cgagaccacg ggggtcaagt ccatggtgct 540
aggccgcttc ctgagtcaga ttcgggaaaa gcttggtcca gaccatctac ccgcggggtc 600
gagccccacc ttgcccaact ggttcgcggt gaccaaagac gcggtaatgg cgccggcggg 660
ggggaacaag gtggtggacg agtgctacat ccccaactac ctcctgccca agactcagcc 720
cgagctgcag tgggcgtgga ctaacatgga ggagtatata agcgcgtgct tgaacctggc 780
cgagcgcaaa cggctcgtgg cgcagcacct gacccacgtc agccagacgc aggagcagaa 840
caaggagaat ctgaacccca attctgacgc gcccgtgatc aggtcaaaaa cctccgcgcg 900
ctatatggag ctggtcgggt ggctggtgga ccggggcatc acctccgaga agcagtggat 960
ccaggaggac caggcctcgt acatctcctt caacgccgcc tccaactcgc ggtcccagat 1020
caaggccgcg ctggacaatg ccggcaagat catggcgctg accaaatccg cgcccgacta 1080
cctggtgggg ccctcgctgc ccgcggacat tacccagaac cgcatctacc gcatcctcgc 1140
tctcaacggc tacgaccctg cctacgccgg ctccgtcttt ctcggctggg ctcagaaaaa 1200
gttcgggaaa cgcaacacca tctggctgtt tggacccgcc accaccggca agaccaacat 1260
tgcggaagcc atcgcccacg ccgtgccctt ctacggctgc gtcaactgga ccaatgagaa 1320
ctttcccttc aatgattgcg tcgacaagat ggtgatctgg tgggaggagg gcaagatgac 1380
ggccaaggtc gtggagtccg ccaaggccat tctcggcggc agcaaggtgc gcgtggacca 1440
aaagtgcaag tcgtccgccc agatcgaccc cacccccgtg atcgtcacct ccaacaccaa 1500
catgtgcgcc gtgattgacg ggaacagcac caccttcgag caccagcagc ctctccagga 1560
ccggatgttt aagttcgaac tcacccgccg tctggagcac gactttggca aggtgacaaa 1620
gcaggaagtc aaagagttct tccgctgggc cagtgatcac gtgaccgagg tggcgcatga 1680
gttttacgtc agaaagggcg gagccagcaa aagacccgcc cccgatgacg cggataaaag 1740
cgagcccaag cgggcctgcc cctcagtcgc ggatccatcg acgtcagacg cggaaggagc 1800
tccggtggac tttgccgaca ggtaccaaaa caaatgttct cgtcacgcgg gcatgcttca 1860
gatgctgttt ccctgcaaaa cgtgcgagag aatgaatcag aatttcaaca tttgcttcac 1920
acacggggtc agagactgct cagagtgttt ccccggcgtg tcagaatctc aaccggtcgt 1980
cagaaagagg acgtatcgga aactctgtgc gattcatcat ctgctggggc gggctcccga 2040
gattgcttgc tcggcctgcg atctggtcaa cgtggacctg gatgactgtg tttctgagca 2100
ataaatgact taaaccaggt atggctgccg atggttatct tccagattgg ctcgaggaca 2160
acctctctga gggcattcgc gagtggtggg cgctgaaacc tggagccccg aagcccaaag 2220
ccaaccagca aaagcaggac gacggccggg gtctggtgct tcctggctac aagtacctcg 2280
gacccttcaa cggactcgac aagggggagc ccgtcaacgc ggcggacgca gcggccctcg 2340
agcacgacaa ggcctacgac cagcagctgc aggcgggtga caatccgtac ctgcggtata 2400
accacgccga cgccgagttt caggagcgtc tgcaagaaga tacgtctttt gggggcaacc 2460
tcgggcgagc agtcttccag gccaagaagc gggttctcga acctctcggt ctggttgagg 2520
aaggcgctaa gacggctcct ggaaagaaga gaccggtaga gccatcaccc cagcgttctc 2580
cagactcctc tacgggcatc ggcaagaaag gccaacagcc cgccagaaaa agactcaatt 2640
ttggtcagac tggcgactca gagtcagttc cagaccctca acctctcgga gaacctccag 2700
cagcgccctc tggtgtggga cctaatacaa tggctgcagg cggtggcgca ccaatggcag 2760
acaataacga aggcgccgac ggagtgggta gttcctcggg aaattggcat tgcgattcca 2820
catggctggg cgacagagtc atcaccacca gcacccgaac ctgggccctg cccacctaca 2880
acaaccacct ctacaagcaa atctccaacg ggacatcggg aggagccacc aacgacaaca 2940
cctacttcgg ctacagcacc ccctgggggt attttgactt taacagattc cactgccact 3000
tttcaccacg tgactggcag cgactcatca acaacaactg gggattccgg cccaagagac 3060
tcagcttcaa gctcttcaac atccaggtca aggaggtcac gcagaatgaa ggcaccaaga 3120
ccatcgccaa taacctcacc agcaccatcc aggtgtttac ggactcggag taccagctgc 3180
cgtacgttct cggctctgcc caccagggct gcctgcctcc gttcccggcg gacgtgttca 3240
tgattcccca gtacggctac ctaacactca acaacggtag tcaggccgtg ggacgctcct 3300
ccttctactg cctggaatac tttccttcgc agatgctgag aaccggcaac aacttccagt 3360
ttacttacac cttcgaggac gtgcctttcc acagcagcta cgcccacagc cagagcttgg 3420
accggctgat gaatcctctg attgaccagt acctgtacta cttgtctcgg actcaaacaa 3480
caggaggcac ggcaaatacg cagactctgg gcttcagcca aggtgggcct aatacaatgg 3540
ccaatcaggc aaagaactgg ctgccaggac cctgttaccg ccaacaacgc gtctcaacga 3600
caaccgggca aaacaacaat agcaactttg cctggactgc tgggaccaaa taccatctga 3660
atggaagaaa ttcattggct aatcctggca tcgctatggc aacacacaaa gacgacgagg 3720
agcgtttttt tcccagtaac gggatcctga tttttggcaa acaaaatgct gccagagaca 3780
atgcggatta cagcgatgtc atgctcacca gcgaggaaga aatcaaaacc actaaccctg 3840
tggctacaga ggaatacggt atcgtggcag ataacttgca gcagcaaaac acggctcctc 3900
aaattggaac tgtcaacagc cagggggcct tacccggtat ggtctggcag aaccgggacg 3960
tgtacctgca gggtcccatc tgggccaaga ttcctcacac ggacggcaac ttccacccgt 4020
ctccgctgat gggcggcttt ggcctgaaac atcctccgcc tcagatcctg atcaagaaca 4080
cgcctgtacc tgcggatcct ccgaccacct tcaaccagtc aaagctgaac tctttcatca 4140
cgcaatacag caccggacag gtcagcgtgg aaattgaatg ggagctgcag aaggaaaaca 4200
gcaagcgctg gaaccccgag atccagtaca cctccaacta ctacaaatct acaagtgtgg 4260
actttgctgt taatacagaa ggcgtgtact ctgaaccccg ccccattggc acccgttacc 4320
tcacccgtaa tctgtaattg cctgttaatc aataaaccgg ttgattcgtt tcagttgaac 4380
tttggtctct gcg 4393
<210>183
<211>738
<212>PRT
<213>腺伴随病毒血清型8的衣壳蛋白
<400>183
Met Ala Ala Asp Gly Tyr Leu Pro Asp Trp Leu Glu Asp Asn Leu Ser
1 5 10 15
Glu Gly Ile Arg Glu Trp Trp Ala Leu Lys Pro Gly Ala Pro Lys Pro
20 25 30
Lys Ala Asn Gln Gln Lys Gln Asp Asp Gly Arg Gly Leu Val Leu Pro
35 40 45
Gly Tyr Lys Tyr Leu Gly Pro Phe Asn Gly Leu Asp Lys Gly Glu Pro
50 55 60
Val Asn Ala Ala Asp Ala Ala Ala Leu Glu His Asp Lys Ala Tyr Asp
65 70 75 80
Gln Gln Leu Gln Ala Gly Asp Asn Pro Tyr Leu Arg Tyr Asn His Ala
85 90 95
Asp Ala Glu Phe Gln Glu Arg Leu Gln Glu Asp Thr Ser Phe Gly Gly
100 105 110
Asn Leu Gly Arg Ala Val Phe Gln Ala Lys Lys Arg Val Leu Glu Pro
115 120 125
Leu Gly Leu Val Glu Glu Gly Ala Lys Thr Ala Pro Gly Lys Lys Arg
130 135 140
Pro Val Glu Pro Ser Pro Gln Arg Ser Pro Asp Ser Ser Thr Gly Ile
145 150 155 160
Gly Lys Lys Gly Gln Gln Pro Ala Arg Lys Arg Leu Asn Phe Gly Gln
165 170 175
Thr Gly Asp Ser Glu Ser Val Pro Asp Pro Gln Pro Leu Gly Glu Pro
180 185 190
Pro Ala Ala Pro Ser Gly Val Gly Pro Asn Thr Met Ala Ala Gly Gly
195 200 205
Gly Ala Pro Met Ala Asp Asn Asn Glu Gly Ala Asp Gly Val Gly Ser
210 215 220
Ser Ser Gly Asn Trp His Cys Asp Ser Thr Trp Leu Gly Asp Arg Val
225 230 235 240
Ile Thr Thr Ser Thr Arg Thr Trp Ala Leu Pro Thr Tyr Asn Asn His
245 250 255
Leu Tyr Lys Gln Ile Ser Asn Gly Thr Ser Gly Gly Ala Thr Asn Asp
260 265 270
Asn Thr Tyr Phe Gly Tyr Ser Thr Pro Trp Gly Tyr Phe Asp Phe Asn
275 280 285
Arg Phe His Cys His Phe Ser Pro Arg Asp Trp Gln Arg Leu Ile Asn
290 295 300
Asn Asn Trp Gly Phe Arg Pro Lys Arg Leu Ser Phe Lys Leu Phe Asn
305 310 315 320
Ile Gln Val Lys Glu Val Thr Gln Asn Glu Gly Thr Lys Thr Ile Ala
325 330 335
Asn Asn Leu Thr Ser Thr Ile Gln Val Phe Thr Asp Ser Glu Tyr Gln
340 345 350
Leu Pro Tyr Val Leu Gly Ser Ala His Gln Gly Cys Leu Pro Pro Phe
355 360 365
Pro Ala Asp Val Phe Met Ile Pro Gln Tyr Gly Tyr Leu Thr Leu Asn
370 375 380
Asn Gly Ser Gln Ala Val Gly Arg Ser Ser Phe Tyr Cys Leu Glu Tyr
385 390 395 400
Phe Pro Ser Gln Met Leu Arg Thr Gly Asn Asn Phe Gln Phe Thr Tyr
405 410 415
Thr Phe Glu Asp Val Pro Phe His Ser Ser Tyr Ala His Ser Gln Ser
420 425 430
Leu Asp Arg Leu Met Asn Pro Leu Ile Asp Gln Tyr Leu Tyr Tyr Leu
435 440 445
Ser Arg Thr Gln Thr Thr Gly Gly Thr Ala Asn Thr Gln Thr Leu Gly
450 455 460
Phe Ser Gln Gly Gly Pro Asn Thr Met Ala Asn Gln Ala Lys Asn Trp
465 470 475 480
Leu Pro Gly Pro Cys Tyr Arg Gln Gln Arg Val Ser Thr Thr Thr Gly
485 490 495
Gln Asn Asn Asn Ser Asn Phe Ala Trp Thr Ala Gly Thr Lys Tyr His
500 505 510
Leu Asn Gly Arg Asn Ser Leu Ala Asn Pro Gly Ile Ala Met Ala Thr
515 520 525
His Lys Asp Asp Glu Glu Arg Phe Phe Pro Ser Asn Gly Ile Leu Ile
530 535 540
Phe Gly Lys Gln Asn Ala Ala Arg Asp Asn Ala Asp Tyr Ser Asp Val
545 550 555 560
Met Leu Thr Ser Glu Glu Glu Ile Lys Thr Thr Asn Pro Val Ala Thr
565 570 575
Glu Glu Tyr Gly Ile Val Ala Asp Asn Leu Gln Gln Gln Asn Thr Ala
580 585 590
Pro Gln Ile Gly Thr Val Asn Ser Gln Gly Ala Leu Pro Gly Met Val
595 600 605
Trp Gln Asn Arg Asp Val Tyr Leu Gln Gly Pro Ile Trp Ala Lys Ile
610 615 620
Pro His Thr Asp Gly Asn Phe His Pro Ser Pro Leu Met Gly Gly Phe
625 630 635 640
Gly Leu Lys His Pro Pro Pro Gln Ile Leu Ile Lys Asn Thr Pro Val
645 650 655
Pro Ala Asp Pro Pro Thr Thr Phe Asn Gln Ser Lys Leu Asn Ser Phe
660 665 670
Ile Thr Gln Tyr Ser Thr Gly Gln Val Ser Val Glu Ile Glu Trp Glu
675 680 685
Leu Gln Lys Glu Asn Ser Lys Arg Trp Asn Pro Glu Ile Gln Tyr Thr
690 695 700
Ser Asn Tyr Tyr Lys Ser Thr Ser Val Asp Phe Ala Val Asn Thr Glu
705 710 715 720
Gly Val Tyr Ser Glu Pro Arg Pro Ile Gly Thr Arg Tyr Leu Thr Arg
725 730 735
Asn Leu
<210>184
<211>735
<212>PRT
<213>vp1,克隆hu.60
<400>184
Met Ala Ala Asp Gly Tyr Leu Pro Asp Trp Leu Glu Asp Thr Leu Ser
1 5 10 15
Glu Gly Ile Arg Gln Trp Trp Lys Leu Lys Pro Gly Pro Pro Pro Pro
20 25 30
Lys Pro Ala Glu Arg His Lys Asp Asp Ser Arg Gly Leu Val Leu Pro
35 40 45
Gly Tyr Lys Tyr Leu Gly Pro Phe Asn Gly Leu Asp Lys Gly Glu Pro
50 55 60
Val asn Glu Ala Asp Ala Ala Ala Leu Glu His Asp Lys Ala Tyr Asp
65 70 75 80
Arg Gln Leu Asp Ser Gly Asp Asn Pro Tyr Leu Lys Tyr Asn His Ala
85 90 95
Asp Ala Glu Phe Gln Glu Arg Leu Lys Glu Asp Thr Ser Phe Gly Gly
100 105 110
Asn Leu Gly Arg Ala Val Phe Gln Ala Lys Lys Arg Val Leu Glu Pro
115 120 125
Leu Gly Leu Val Glu Glu Pro Val Lys Thr Ala Pro Gly Lys Lys Arg
130 135 140
Pro Val Glu His Ser Pro Val Glu Pro Asp Ser Ser Ser Gly Thr Gly
145 150 155 160
Lys Ala Gly Gln Gln Pro Ala Arg Lys Arg Leu Asn Phe Gly Gln Thr
165 170 175
Gly Asp Ala Asp Ser Val Pro Asp Pro Gln Pro Leu Gly Gln Pro Pro
180 185 190
Ala Ala Pro Ser Gly Leu Gly Ser Thr Thr Met Ala Thr Gly Ser Gly
195 200 205
Ala Pro Met Ala Asp Asn Asn Glu Gly Ala Asp Gly Val Gly Asn Ser
210 215 220
Ser Gly Asn Trp His Cys Asp Ser Gln Trp Leu Gly Asp Arg Val Ile
225 230 235 240
Thr Thr Ser Thr Arg Thr Trp Ala Leu Pro Thr Tyr Asn Asn His Leu
245 250 255
Tyr Lys Gln Ile Ser Ser Gln Ser Gly Ala Ser Asn Asp Asn His Tyr
260 265 270
Phe Gly Tyr Ser Thr Pro Trp Gly Tyr Phe Asp Phe Asn Arg Phe His
275 280 285
Cys His Phe Ser Pro Arg Asp Trp Gln Arg Leu Ile Asn Asn Asn Trp
290 295 300
Gly Phe Arg Pro Lys Arg Leu Asn Phe Lys Leu Phe Asn Ile Gln Val
305 310 315 320
Lys Glu Val Thr Gln Asn Asp Gly Thr Thr Thr Ile Ala Asn Asn Leu
325 330 335
Thr Ser Thr Val Gln Val Phe Thr Asp Ser Glu Tyr Gln Leu Pro Tyr
340 345 350
Val Leu Gly Ser Ala His Gln Gly Cys Leu Pro Pro Phe Pro Ala Asp
355 360 365
Val Phe Met Val Pro Gln Tyr Gly Tyr Leu Thr Leu Asn Asn Gly Ser
370 375 380
Gln Ala Val Gly Arg Ser Ser Phe Tyr Cys Leu Glu Tyr Phe Pro Ser
385 390 395 400
Gln Met Leu Arg Thr Gly Asn Asn Phe Gln Phe Ser Tyr Thr Phe Glu
405 410 415
Asp Val Pro Phe His Ser Ser Tyr Ala His Ser Gln Ser Leu Asp Arg
420 425 430
Leu Met Asn Pro Leu Val Asp Gln Tyr Leu Tyr Tyr Leu Asn Lys Thr
435 440 445
Gln Thr Asn Ser Gly Thr Leu Gln Gln Ser Arg Leu Leu Phe Ser Gln
450 455 460
Ala Gly Pro Thr Asn Met Ser Leu Gln Ala Lys Asn Trp Leu Pro Gly
465 470 475 480
Pro Cys Tyr Arg Gln Gln Arg Leu Ser Lys Gln Ala Asn Asp Asn Asn
485 490 495
Asn Ser Asn Phe Pro Trp Thr Ala Ala Thr Lys Tyr His Leu Asn Gly
500 505 510
Arg Asp Ser Leu Val Asn Pro Gly Pro Ala Met Ala Ser His Lys Asp
515 520 525
Asp Glu Glu Lys Phe Phe Pro Met His Gly Thr Leu Ile Phe Gly Lys
530 535 540
Gln Gly Thr Asn Ala Asn Asp Ala Asp Leu Glu Asn Val Met Ile Thr
545 550 555 560
Asp Glu Glu Glu Ile Arg Thr Thr Asn Pro Val Ala Thr Glu Gln Tyr
565 570 575
Gly Thr Val Ser Asn Asn Leu Gln Asn Ser Asn Thr Gly Pro Thr Thr
580 585 590
Gly Thr Val Asn His Gln Gly Ala Leu Pro Gly Met Val Trp Gln Asp
595 600 605
Arg Asp Val Tyr Leu Gln Gly Pro Ile Trp Ala Lys Ile Pro His Thr
610 615 620
Asp Gly His Phe His Pro Ser Pro Leu Met Gly Gly Phe Gly Leu Lys
625 630 635 640
His Pro Pro Pro Gln Ile Met Ile Lys Asn Thr Pro Val Pro Ala Asn
645 650 655
Pro Pro Thr Asn Phe Ser Ser Ala Lys Phe Ala Ser Phe Ile Thr Gln
660 665 670
Tyr Ser Thr Gly Gln Val Ser Val Glu Ile Glu Trp Glu Leu Gln Lys
675 680 685
Glu Asn Ser Lys Arg Trp asn Pro Glu Ile Gln Tyr Thr Ser Asn Tyr
690 695 700
Asn Lys Ser Val Asn Val Asp Phe Thr Val Asp Thr Asn Gly Val Tyr
705 710 715 720
Ser Glu Pro Arg Pro Ile Gly Thr Arg Tyr Leu Thr Arg Asn Leu
725 730 735
<210>185
<211>735
<212>PRT
<213>vp1,克隆hu.61
<400>185
Met Ala Ala Asp Gly Tyr Leu Pro Asp Trp Leu Glu Asp Thr Leu Ser
1 5 10 15
Glu Gly Ile Arg Gln Trp Trp Lys Leu Lys Pro Gly Pro Pro Pro Pro
20 25 30
Lys Pro Ala Glu Arg His Lys Asp Asp Ser Arg Gly Leu Val Leu Pro
35 40 45
Gly Tyr Lys Tyr Leu Gly Pro Phe Asn Gly Leu Asp Lys Gly Glu Pro
50 55 60
Val Asn Glu Ala Asp Ala Ala Ala Leu Glu His Asp Lys Ala Tyr Asp
65 70 75 80
Arg Gln Leu Asp Ser Gly Asp Asn Pro Tyr Leu Lys Tyr Asn His Ala
85 90 95
Asp Ala Glu Phe Gln Glu Arg Leu Lys Glu Asp Thr Ser Phe Gly Gly
100 105 110
Asn Leu Gly Arg ala Val Phe Gln Ala Lys Lys Arg Val Leu Glu Pro
115 120 125
Leu Gly Leu Val Glu Glu Pro Val Lys Thr Ala Pro Gly Lys Lys Arg
130 135 140
Pro Val Glu His Pro Pro Val Glu Pro Asp Ser Ser Ser Gly Thr Gly
145 150 155 160
Lys Ala Gly Gln Gln Pro Ala Arg Lys Arg Leu asn Phe Gly Gln Thr
165 170 175
Gly Asp Ala Asp Ser Val Pro Asp Pro Gln Pro Leu Gly Gln Pro Pro
180 185 190
Ala Ala Pro Ser Gly Leu Gly Ser Thr Thr Met Ala Thr Gly Ser Gly
195 200 205
Ala Pro Met Ala Asp Asn Asn Glu Gly Ala Asp Gly Val Gly Asn Ser
210 215 220
Ser Gly Asn Trp His Cys Asp Ser Gln Trp Leu Gly Asp Arg Val Ile
225 230 235 240
Thr Thr Ser Thr Arg Thr Trp Ala Leu Pro Thr Tyr Asn Asn His Leu
245 250 255
Tyr Lys Gln Ile Ser Ser Gln Ser Gly Ala Ser Asn Asp Asn His Tyr
260 265 270
Phe Gly Tyr Ser Thr Pro Trp Gly Tyr Phe Asp Phe Asn Arg Phe His
275 280 285
Cys His Phe Ser Pro Arg Asp Trp Gln Arg Leu Ile Asn Asn Asn Trp
290 295 300
Gly Phe Arg Pro Lys Arg Leu Asn Phe Lys Leu Phe Asn Ile Gln Val
305 310 315 320
Lys Glu Val Thr Gln Asn Asp Gly Thr Thr Thr Ile Ala Asn Asn Leu
325 330 335
Thr Ser Thr Val Gln Val Phe Thr Asp Ser Glu Tyr Gln Leu Pro Tyr
340 345 350
Val Leu Gly Ser Ala His Gln Gly Cys Leu Pro Pro Phe Pro Ala Asp
355 360 365
Val Phe Met Val Pro Gln Tyr Gly Tyr Leu Thr Leu Asn Asn Gly Ser
370 375 380
Gln Ala Val Gly Arg Ser Ser Phe Tyr Cys Leu Glu Tyr Phe Pro Ser
385 390 395 400
Gln Met Leu Arg Thr Gly Asn Asn Phe Gln Phe Ser Tyr Thr Phe Glu
405 410 415
Asp Val Pro Phe His Ser Ser Tyr Ala His Ser Gln Ser Leu Asp Arg
420 425 430
Leu Met Asn Pro Leu Ile Asp Gln Tyr Leu Tyr Tyr Leu Asn Lys Thr
435 440 445
Gln Thr Asn Ser Gly Thr Leu Gln Gln Ser Arg Leu Leu Phe Ser Gln
450 455 460
Ala Gly Pro Thr Asn Met Ser Leu Gln Ala Lys Asn Arg Leu Pro Gly
465 470 475 480
Pro Cys Tyr Arg Gln Gln Arg Leu Ser Lys Gln Ala Asn Asp Asn Asn
485 490 495
Asn Ser Asn Phe Pro Trp Thr Ala Ala Thr Lys Tyr His Leu Asn Gly
500 505 510
Arg Asp Ser Leu Val Asn Pro Gly Pro Ala Met Ala Ser His Lys Asp
515 520 525
Asp Glu Glu Lys Phe Phe Pro Met His Gly Thr Leu Ile Phe Gly Lys
530 535 540
Gln Gly Thr Asn Ala Asn Asp Ala Asp Leu Glu Asn Val Met Ile Thr
545 550 555 560
Asp Glu Glu Glu Ile Arg Thr Thr Asn Pro Val Ala Thr Glu Gln Tyr
565 570 575
Gly Thr Val Ser Asn Asn Leu Gln Asn Ser Asn Thr Gly Pro Thr Thr
580 585 590
Gly Thr Val Asn His Gln Gly Ala Leu Pro Gly Met Val Trp Gln Asp
595 600 605
Arg Asp Val Tyr Leu Gln Gly Pro Ile Trp Ala Lys Ile Pro His Thr
610 615 620
Asp Gly His Phe His Pro Ser Pro Leu Val Gly Gly Phe Gly Leu Lys
625 630 635 640
His Pro Pro Pro Gln Ile Met Ile Lys Asn Thr Pro Val Pro Ala Asn
645 650 655
Pro Pro Thr Asn Phe Ser Ser Ala Lys Phe Ala Ser Phe Ile Thr Gln
660 665 670
Tyr Ser Thr Gly Gln Val Ser Val Glu Ile Glu Trp Glu Leu Gln Lys
675 680 685
Glu Asn Ser Lys Arg Trp Asn Pro Glu Ile Gln Tyr Thr Ser Asn Tyr
690 695 700
Asn Lys Ser Val Asn Val Asp Phe Thr Val Asp Thr Asn Gly Val Tyr
705 710 715 720
Ser Glu Pro Arg Pro Ile Gly Thr Arg Tyr Leu Thr Arg Asn Leu
725 730 735
<210>186
<211>734
<212>PRT
<213>vp1,克隆hu.53
<400>186
Met Ala Ala Asp Gly Tyr Leu Pro Asp Trp Leu Glu Asp Thr Leu Ser
1 5 10 15
Glu Gly Ile Arg Gln Trp Trp Lys Leu Lys Pro Gly Pro Pro Pro Pro
20 25 30
Lys Pro Ala Glu Arg His Lys Asp Asp Ser Arg Gly Leu Val Leu Pro
35 40 45
Gly Tyr Lys Tyr Leu Gly Pro Phe Asn Gly Leu Asp Lys Gly Glu Pro
50 55 60
Val Asn Glu Ala Asp Ala Ala Ala Leu Glu His Asp Lys Ala Tyr Asp
65 70 75 80
Arg Gln Leu Asp Ser Gly Asp Asn Pro Tyr Leu Lys Tyr Asn His Ala
85 90 95
Asp Ala Glu Phe Gln Glu Arg Leu Lys Glu Asp Thr Ser Phe Gly Gly
100 105 110
Asn Leu Gly Arg Ala Val Phe Gln Ala Lys Lys Arg Val Leu Glu Pro
115 120 125
Leu Gly Leu Val Glu Glu Pro Val Lys Thr Ala Pro Gly Lys Lys Arg
130 135 140
Pro Val Glu His Ser Pro Ala Glu Pro Asp Ser Ser Ser Gly Thr Gly
145 150 155 160
Lys Ala Gly Gln Gln Pro Ala Arg Lys Arg Leu Asn Phe Gly Gln Thr
165 170 175
Gly Asp Ala Asp Ser Val Pro Asp Pro Gln Pro Leu Arg Gln Pro Pro
180 185 190
Ala Ala Pro Thr Ser Leu Gly Ser Thr Thr Met Ala Thr Gly Ser Gly
195 200 205
Ala Pro Met Ala Asp Asn Asn Glu Gly Ala Asp Gly Val Gly Asn Ser
210 215 220
Ser Gly Asn Trp His Cys Asp Ser Gln Trp Leu Gly Asp Arg Val Ile
225 230 235 240
Thr Thr Ser Thr Arg Thr Trp Ala Leu Pro Thr Tyr Asn Asn His Leu
245 250 255
Tyr Lys Gln Ile Ser Ser Gln Ser Gly Ala Ser Asn Asp Asn His Tyr
260 265 270
Phe Gly Tyr Ser Thr Pro Trp Gly Tyr Phe Asp Phe Asn Arg Phe His
275 280 285
Cys His Phe Ser Pro Arg Asp Trp Gln Arg Leu Ile Asn Asn Asn Trp
290 295 300
Gly Phe Arg Pro Lys Arg Leu Asn Phe Lys Leu Phe Asn Ile Gln Val
305 310 315 320
Lys Glu Val Thr Gln Asn Asp Gly Thr Thr Thr Ile Ala Asn Asn Leu
325 330 335
Thr Ser Thr Val Gln Val Phe Thr Asp Ser Glu Tyr Gln Leu Pro Tyr
340 345 350
Val Leu Gly Ser Ala His Gln Gly Cys Leu Pro Pro Phe Pro Ala Asp
355 360 365
Val Phe Met Val Pro Gln Tyr Gly Tyr Leu Thr Leu Asn Asn Gly Ser
370 375 380
Gln Ala Val Gly Arg Ser Ser Phe Tyr Cys Leu Glu Tyr Phe Pro Ser
385 390 395 400
Gln Met Leu Arg Thr Gly Asn Asn Phe Gln Phe Ser Tyr Thr Phe Glu
405 410 415
Asp Val Pro Phe His Ser Ser Tyr Ala His Ser Gln Ser Leu Asp Arg
420 425 430
Leu Met Asn Pro Leu Ile Asp Gln Tyr Leu Tyr Tyr Leu Asn Arg Thr
435 440 445
Gln Thr Ala Ser Gly Thr Gln Gln Ser Arg Leu Leu Phe Ser Gln Ala
450 455 460
Gly Pro Thr Ser Met Ser Leu Gln Ala Lys Asn Trp Leu Pro Gly Pro
465 470 475 480
Cys Tyr Arg Gln Gln Arg Leu Ser Lys Gln Ala Asn Asp Asn Asn Asn
485 490 495
Ser Asn Phe Pro Trp Thr Gly Ala Thr Lys Tyr Tyr Leu Asn Gly Arg
500 505 510
Asp Ser Leu Val Asn Pro Gly Pro Ala Met Ala Ser His Lys Asp Asp
515 520 525
Glu Glu Lys Phe Phe Pro Met His Gly Thr Leu Ile Phe Gly Lys Glu
530 535 540
Gly Thr Asn Ala Thr Asn Ala Glu Leu Glu Asn Val Met Ile Thr Asp
545 550 555 560
Glu Glu Glu Ile Arg Thr Thr Asn Pro Val Ala Thr Glu Gln Tyr Gly
565 570 575
Tyr Val Ser Asn Asn Leu Gln Asn Ser Asn Thr Ala Ala Ser Thr Glu
580 585 590
Thr Val Asn His Gln Gly Ala Leu Pro Gly Met Val Trp Gln Asp Arg
595 600 605
Asp Val Tyr Leu Gln Gly Pro Ile Trp Ala Lys Ile Pro His Thr Asp
610 615 620
Gly His Phe His Pro Ser Pro Leu Met Gly Gly Phe Gly Leu Lys His
625 630 635 640
Pro Pro Pro Gln Ile Met Ile Lys Asn Thr Pro Val Pro Ala Asn Pro
645 650 655
Pro Thr Asn Phe Ser Ser Ala Lys Phe Ala Ser Phe Ile Thr Gln Tyr
660 665 670
Ser Thr Gly Gln Val Ser Val Glu Ile Glu Trp Glu Leu Gln Lys Glu
675 680 685
Asn Ser Lys Arg Trp Asn Pro Glu Ile Gln Tyr Thr Ser Asn Tyr Asn
690 695 700
Lys Ser Val Asn Val Asp Phe Thr Val Asp Thr Asn Gly Val Tyr Ser
705 710 715 720
Glu Pro Arg Pro Ile Gly Thr Arg Tyr Leu Thr Arg Asn Leu
725 730
<210>187
<211>734
<212>PRT
<213>vp1,克隆hu.55
<400>187
Met Ala Ala Asp Gly Tyr Leu Pro Asp Trp Leu Glu Asp Thr Leu Ser
1 5 10 15
Glu Gly Ile Arg Gln Trp Trp Lys Leu Lys Pro Gly Pro Pro Pro Pro
20 25 30
Lys Pro Ala Glu Arg His Lys Asp Asp Ser Arg Gly Leu Val Leu Pro
35 40 45
Gly Tyr Lys Tyr Leu Gly Pro Phe Asn Gly Leu Asp Lys Gly Glu Pro
50 55 60
Val Asn Glu Ala Asp Ala Ala Ala Leu Glu His Asp Lys Ala Tyr Asp
65 70 75 80
Arg Gln Leu Asp Ser Gly Asp Asn Pro Tyr Leu Lys Tyr Asn His Ala
85 90 95
Asp Ala Glu Phe Gln Glu Arg Leu Lys Glu Asp Thr Ser Phe Gly Gly
100 105 110
Asn Leu Gly Arg Ala Val Phe Gln Ala Lys Lys Arg Val Leu Glu Pro
115 120 125
Leu Gly Leu Val Glu Glu Pro Val Lys Thr Ala Pro Gly Lys Lys Arg
130 135 140
Pro Val Glu His Ser Pro Ala Glu Pro Asp Ser Ser Ser Gly Thr Gly
145 150 155 160
Lys Ala Gly Gln Gln Pro Ala Arg Lys Arg Leu Asn Phe Gly Gln Thr
165 170 175
Gly Asp Ala Asp Ser Val Pro Asp Pro Gln Pro Leu Gly Gln Pro Pro
180 185 190
Ala Ala Pro Thr Ser Leu Gly Ser Thr Thr Met Ala Thr Gly Ser Gly
195 200 205
Ala Pro Met Ala Asp Asn Asn Glu Gly Ala Asp Gly Val Gly Asn Ser
210 215 220
Ser Gly Asn Trp His Cys Asp Ser Gln Trp Leu Gly Asp Arg Val Ile
225 230 235 240
Thr Thr Ser Thr Arg Thr Trp Ala Leu Pro Thr Tyr Asn Asn His Leu
245 250 255
Tyr Lys Gln Ile Ser Ser Gln Ser Gly Ala Ser Asn Asp Asn His Tyr
260 265 270
Phe Gly Tyr Ser Thr Pro Trp Gly Tyr Phe Asp Phe Asn Arg Phe His
275 280 285
Cys His Phe Ser Pro Arg Asp Trp Gln Arg Leu Ile Asn Asn Asn Trp
290 295 300
Gly Phe Arg Pro Lys Arg Leu Asn Phe Lys Leu Phe Asn Ile Gln Val
305 310 315 320
Lys Glu Val Thr Gln Asn Asp Gly Thr Thr Thr Ile Ala Asn Asn Leu
325 330 335
Thr Ser Thr Val Gln Val Phe Thr Asp Ser Glu Tyr Gln Leu Pro Tyr
340 345 350
Val Leu Gly Ser Ala His Gln Gly Cys Leu Pro Pro Phe Pro Ala Asp
355 360 365
Val Phe Met Val Pro Gln Tyr Gly Tyr Leu Thr Leu Asn Asn Gly Ser
370 375 380
Gln Ala Val Gly Arg Ser Ser Phe Tyr Cys Leu Glu Cys Phe Pro Ser
385 390 395 400
Gln Met Leu Arg Thr Gly Asn Asn Phe Gln Phe Ser Tyr Thr Phe Glu
405 410 415
Asp Val Pro Phe His Ser Ser Tyr Ala His Ser Gln Ser Leu Asp Arg
420 425 430
Leu Met Asn Pro Leu Ile Asp Gln Tyr Leu Tyr Tyr Leu Asn Arg Thr
435 440 445
Gln Thr Ala Ser Gly Thr Gln Gln Ser Arg Leu Leu Phe Ser Gln Ala
450 455 460
Gly Pro Thr Ser Met Ser Leu Gln Ala Lys Asn Trp Leu Pro Gly Pro
465 470 475 480
Cys Tyr Arg Gln Gln Arg Leu Ser Lys Gln Ala Asn Asp Asn Asn Asn
485 490 495
Ser Asn Phe Pro Trp Thr Gly Ala Thr Lys Tyr His Leu Asn Gly Arg
500 505 510
Asp Ser Leu Val Asn Pro Gly Pro Ala Met Ala Ser His Lys Asp Asp
515 520 525
Glu Glu Lys Phe Phe Pro Met His Gly Thr Leu Ile Phe Gly Lys Glu
530 535 540
Gly Thr Asn Ala Thr Asn Ala Glu Leu Glu Asn Val Met Ile Thr Asp
545 550 555 560
Glu Glu Glu Ile Arg Thr Thr Asn Pro Val Ala Thr Glu Gln Tyr Gly
565 570 575
Tyr Val Ser Asn Asn Leu Gln Asn Ser Asn Thr Ala Ala Ser Thr Glu
580 585 590
Thr Val Asn His Gln Gly Ala Leu Pro Gly Met Val Trp Gln Asp Arg
595 600 605
Asp Val Tyr Leu Gln Gly Pro Ile Trp Ala Lys Ile Pro His Thr Asp
610 615 620
Gly His Phe His Pro Ser Pro Leu Met Gly Gly Phe Gly Leu Lys His
625 630 635 640
Pro Pro Pro Gln Ile Met Ile Lys Asn Thr Pro Val Pro Ala Asn Pro
645 650 655
Pro Thr Asn Phe Ser Ser Ala Lys Phe Ala Ser Phe Ile Thr Gln Tyr
660 665 670
Ser Thr Gly Gln Val Ser Val Glu Ile Glu Trp Glu Leu Gln Lys Glu
675 680 685
Asn Ser Lys Arg Trp Asn Pro Glu Ile Gln Tyr Thr Ser Asn Tyr Asn
690 695 700
Lys Ser Val Asn Val Asp Phe Thr Val Asp Thr Asn Gly Val Tyr Ser
705 710 715 720
Glu Pro Arg Pro Ile Gly Thr Arg Tyr Leu Thr Arg Asn Leu
725 730
<210>188
<211>734
<212>PRT
<213>vp1,克隆hu.54
<400>188
Met Ala Ala Asp Gly Tyr Leu Pro Asp Trp Leu Glu Asp Thr Leu Ser
1 5 10 15
Glu Gly Ile Arg Gln Trp Trp Lys Leu Lys Pro Gly Pro Pro Pro Pro
20 25 30
Lys Pro Ala Glu Arg His Lys Asp Asp Ser Arg Gly Leu Val Leu Pro
35 40 45
Gly Tyr Lys Tyr Leu Gly Pro Phe Asn Gly Leu Asp Lys Gly Glu Pro
50 55 60
Val Asn Glu Ala Asp Ala Ala Ala Leu Glu His Asp Lys Ala Tyr Asp
65 70 75 80
Arg Gln Leu Asp Ser Gly Asp Asn Pro Tyr Leu Lys Tyr Asn His Ala
85 90 95
Asp Ala Glu Phe Gln Glu Arg Leu Lys Glu Asp Thr Ser Phe Gly Gly
100 105 110
Asn Leu Gly Arg Ala Val Phe Gln Ala Lys Lys Arg Val Leu Glu Pro
115 120 125
Leu Gly Leu Val Glu Glu Pro Val Lys Thr Ala Pro Gly Lys Lys Arg
130 135 140
Pro Val Glu His Ser Pro Ala Glu Pro Asp Ser Ser Ser Gly Thr Gly
145 150 155 160
Lys Ala Gly Gln Gln Pro Ala Arg Lys Arg Leu Asn Phe Gly Gln Thr
165 170 175
Gly Asp Ala Asp Ser Val Pro Asp Pro Gln Pro Leu Gly Gln Pro Pro
180 185 190
Ala Ala Pro Thr Ser Leu Gly Ser Thr Thr Met Ala Thr Gly Ser Gly
195 200 205
Ala Pro Met Ala Asp Asn Asn Glu Gly Ala Asp Gly Val Gly Asn Ser
210 215 220
Ser Gly Asn Trp His Cys Asp Ser Gln Trp Leu Gly Asp Arg Val Ile
225 230 235 240
Thr Thr Ser Thr Arg Thr Trp Ala Leu Pro Thr Tyr Asn Asn His Leu
245 250 255
Tyr Lys Gln Ile Ser Ser Gln Ser Gly Ala Ser Asn Asp Asn His Tyr
260 265 270
Phe Gly Tyr Ser Thr Pro Trp Gly Tyr Phe Asp Phe Asn Arg Phe His
275 280 285
Cys Arg Phe Ser Pro Arg Asp Trp Gln Arg Leu Ile Asn Asn Asn Trp
290 295 300
Gly Phe Arg Pro Lys Arg Leu Asn Phe Lys Leu Phe Asn Ile Gln Val
305 310 315 320
Lys Glu Val Thr Gln Asn Asp Gly Thr Thr Thr Ile Ala Asn Asn Leu
325 330 335
Thr Ser Thr Val Gln Val Phe Thr Asp Ser Glu Tyr Gln Leu Pro Tyr
340 345 350
Val Leu Gly Ser Ala His Gln Gly Cys Leu Pro Pro Phe Pro Ala Asp
355 360 365
Val Phe Met Val Pro Gln Tyr Gly Tyr Leu Thr Leu Asn Asn Gly Ser
370 375 380
Gln Ala Val Gly Arg Ser Ser Phe Tyr Cys Leu Glu Tyr Phe Pro Ser
385 390 395 400
Gln Met Leu Arg Thr Gly Asn Asn Phe Gln Phe Ser Tyr Thr Phe Glu
405 410 415
Asp Val Pro Phe His Ser Ser Tyr Ala His Ser Gln Gly Leu Asp Arg
420 425 430
Leu Met Asn Pro Leu Ile Asp Gln Tyr Leu Tyr Tyr Leu Asn Arg Thr
435 440 445
Gln Thr Ala Ser Gly Thr Gln Gln Ser Arg Leu Leu Phe Ser Gln Ala
450 455 460
Gly Pro Thr Ser Met Ser Leu Gln Ala Lys Asn Trp Leu Pro Gly Pro
465 470 475 480
Cys Tyr Arg Gln Gln Arg Leu Ser Lys Gln Ala Asn Asp Asn Asn Asn
485 490 495
Ser Asn Phe Pro Trp Thr Gly Ala Thr Lys Tyr His Leu Asn Gly Gly
500 505 510
Asp Ser Leu Val Asn Pro Gly Pro Ala Met Ala Ser His Lys Asp Asp
515 520 525
Glu Glu Lys Phe Phe Pro Met His Gly Thr Leu Ile Phe Gly Lys Glu
530 535 540
Gly Thr Asn Ala Thr Asn Ala Glu Leu Glu Asn Val Met Ile Thr Asp
545 550 555 560
Glu Glu Glu Ile Arg Thr Thr Asn Pro Val Ala Thr Glu Gln Tyr Gly
565 570 575
Tyr Val Ser Asn Asn Leu Gln Asn Ser Asn Thr Ala Ala Ser Thr Glu
580 585 590
Thr Val Asn His Gln Gly Ala Leu Pro Gly Met Val Trp Gln Asp Arg
595 600 605
Asp Val Tyr Leu Arg Gly Pro Ile Trp Ala Lys Ile Pro His Ala Asp
610 615 620
Gly His Phe His Pro Ser Pro Leu Met Gly Gly Phe Gly Leu Lys His
625 630 635 640
Pro Pro Pro Gln Ile Met Ile Lys Asn Thr Pro Val Pro Ala Asn Pro
645 650 655
Pro Thr Asn Phe Ser Ser Ala Lys Phe Ala Ser Phe Ile Thr Gln Tyr
660 665 670
Ser Thr Gly Gln Val Ser Val Glu Ile Glu Trp Glu Leu Gln Lys Glu
675 680 685
Asn Ser Lys Arg Trp Asn Pro Glu Ile Gln Tyr Thr Ser Asn Tyr Asn
690 695 700
Lys Ser Val Asn Val Asp Phe Thr Val Asp Thr Asn Gly Val Tyr Ser
705 710 715 720
Glu Pro Arg Pro Ile Gly Thr Arg Tyr Leu Thr Arg Asn Leu
725 730
<210>189
<211>735
<212>PRT
<213>vp1,克隆hu.49
<400>189
Met Ala Ala Asp Gly Tyr Leu Pro Asp Trp Leu Lys Asp Thr Leu Ser
1 5 10 15
Glu Gly Ile Arg Gln Trp Trp Lys Leu Lys Pro Gly Pro Pro Pro Pro
20 25 30
Lys Pro Ala Glu Arg His Lys Asp Asp Ser Gly Gly Leu Val Leu Pro
35 40 45
Gly Tyr Lys Tyr Leu Gly Pro Phe Asn Gly Leu Asp Lys Gly Glu Pro
50 55 60
Val Asn Glu Ala Asp Ala Ala Ala Leu Glu Tyr Asp Lys Ala Tyr Asp
65 70 75 80
Arg Gln Leu Asp Ser Gly Asp Asn Pro Tyr Leu Lys Tyr Asn His Ala
85 90 95
Asp Ala Glu Phe Gln Glu Arg Leu Lys Glu Asp Thr Ser Phe Gly Gly
100 105 110
Asn Leu Gly Arg Ala Val Phe Gln Ala Lys Lys Arg Val Leu Glu Pro
115 120 125
Leu Gly Leu Val Glu Glu Pro Val Lys Thr Ala Pro Gly Lys Lys Arg
130 135 140
Pro Val Glu His Ser Pro Val Glu Pro Asp Ser Ser Ser Gly Thr Gly
145 150 155 160
Lys Ala Gly Gln Gln Pro Ala Arg Lys Arg Leu Asn Phe Gly Gln Thr
165 170 175
Gly Asp Ala Asp Ser Val Pro Asp Pro Gln Pro Leu Gly Gln Pro Pro
180 185 190
Ala Ala Pro Ser Gly Leu Gly Thr Asn Thr Met Ala Thr Gly Ser Gly
195 200 205
Ala Pro Met Ala Asp Asn Asn Glu Gly Ala Asp Gly Val Gly Asn Ser
210 215 220
Ser Gly Ser Trp His Cys Asp Ser Thr Trp Met Gly Asp Arg Val Ile
225 230 235 240
Thr Thr Ser Thr Arg Thr Trp Ala Leu Pro Thr Tyr Asn Asn His Leu
245 250 255
Tyr Lys Gln Ile Ser Ser Gln Ser Gly Ala Ser Asn Asp Asn His Tyr
260 265 270
Phe Gly Tyr Ser Thr Pro Trp Gly Tyr Phe Asp Phe Asn Arg Phe His
275 280 285
Cys His Phe Ser Pro Arg Asp Trp Gln Arg Leu Ile Asn Asn Asn Trp
290 295 300
Gly Phe Arg Pro Lys Arg Leu Asn Phe Lys Leu Phe Asn Ile Gln Val
305 310 315 320
Lys Glu Val Thr Gln Asn Asp Gly Thr Thr Thr Ile Ala Asn Asn Leu
325 330 335
Thr Ser Thr Val Gln Val Phe Thr Asp Ser Glu Tyr Gln Leu Pro Tyr
340 345 350
Val Pro Gly Ser Ala His Gln Gly Cys Leu Pro Pro Phe Pro Ala Asp
355 360 365
Val Phe Met Val Pro Gln Tyr Gly Tyr Leu Thr Leu Asn Asn Gly Ser
370 375 380
Gln Ala Val Gly Arg Ser Ser Phe Tyr Cys Leu Glu Tyr Phe Pro Ser
385 390 395 400
Gln Met Leu Arg Thr Gly Asn Asn Phe Thr Phe Ser Tyr Thr Phe Glu
405 410 415
Asp Val Pro Phe His Ser Ser Tyr Ala His Ser Gln Ser Leu Asp Arg
420 425 430
Leu Met Asn Pro Leu Ile Asp Gln Tyr Leu Tyr Tyr Leu Ser Arg Thr
435 440 445
Asn Thr Pro Ser Gly Thr Thr Thr Gln Ser Arg Leu Gln Phe Ser Gln
450 455 460
Ala Gly Ala Ser Asp Ile Arg Asp Gln Ser Arg Asn Trp Leu Pro Gly
465 470 475 480
Pro Cys Tyr Arg Gln Gln Arg Val Ser Lys Thr Ala Ala Asp Asn Asn
485 490 495
Asn Ser Glu Tyr Ser Trp Thr Gly Ala Thr Lys Tyr His Leu Asn Gly
500 505 510
Arg Asp Ser Leu Val Asn Pro Gly Pro Ala Met Ala Ser His Lys Asp
515 520 525
Asp Glu Glu Lys Phe Phe Pro Gln Ser Gly Val Leu Ile Phe Gly Lys
530 535 540
Gln Gly Ser Glu Lys Thr Asn Val Asp Ile Glu Lys Val Met Ile Thr
545 550 555 560
Asp Glu Glu Glu Ile Arg Thr Thr Asn Pro Val Ala Thr Glu Gln Tyr
565 570 575
Gly Ser Val Ser Thr Asn Leu Gln Ser Gly Asn Thr Gln Ala Ala Thr
580 585 590
Ala Asp Val Asn Thr Gln Gly Val Leu Pro Gly Met Val Trp Gln Asp
595 600 605
Arg Asp Val His Leu Gln Gly Pro Ile Trp Ala Lys Ile Pro His Thr
610 615 620
Asp Gly His Phe His Pro Ser Pro Leu Met Gly Gly Phe Gly Leu Lys
625 630 635 640
His Pro Pro Pro Gln Ile Leu Ile Lys Asn Thr Pro Val Pro Ala Asn
645 650 655
Pro Ser Thr Thr Phe Ser Ala Ala Lys Phe Ala Ser Phe Ile Thr Gln
660 665 670
Tyr Ser Thr Gly Gln Val Ser Val Glu Ile Glu Trp Glu Leu Gln Lys
675 680 685
Glu Asn Ser Lys Arg Trp Asn Pro Glu Ile Gln Tyr Thr Ser Asn Tyr
690 695 700
Asn Lys Ser Val Asn Val Asp Phe Thr Val Asp Thr Asn Gly Val Tyr
705 710 715 720
Ser Glu Pro Arg Pro Ile Gly Thr Arg Tyr Leu Thr Arg Asn Leu
725 730 735
<210>190
<211>735
<212>PRT
<213>vp1,克隆hu.51
<400>190
Met Ala Ala Asp Gly Tyr Leu Pro Asp Trp Leu Glu Asp Thr Leu Ser
1 5 10 15
Glu Gly Ile Arg Gln Trp Trp Lys Leu Lys Pro Gly Pro Pro Pro Pro
20 25 30
Lys Pro Ala Glu Arg His Lys Asp Asp Ser Arg Gly Leu Val Leu Pro
35 40 45
Gly Tyr Lys Tyr Leu Gly Pro Phe Asn Gly Leu Asp Lys Gly Glu Pro
50 55 60
Val Asn Glu Ala Asp Ala Ala Ala Leu Glu His Asp Lys Ala Tyr Asp
65 70 75 80
Arg Gln Leu Asp Ser Gly Asp Asn Pro Tyr Leu Lys Tyr Asn His Ala
85 90 95
Asp Ala Glu Phe Gln Glu Arg Leu Lys Glu Asp Thr Ser Phe Gly Gly
100 105 110
Asn Leu Gly Arg Ala Val Phe Gln Ala Lys Lys Arg Val Leu Glu Pro
115 120 125
Leu Gly Leu Val Gly Glu Pro Val Lys Thr Ala Pro Gly Lys Lys Arg
130 135 140
Pro Val Glu His Ser Pro Val Glu Pro Asp Ser Ser Ser Gly Thr Gly
145 150 155 160
Lys Ala Gly Gln Gln Pro Ala Arg Lys Arg Leu Asn Phe Gly Gln Thr
165 170 175
Gly Asp Ala Asp Ser Val Pro Asp Pro Gln Pro Leu Gly Gln Pro Pro
180 185 190
Ala Ala Pro Ser Gly Leu Gly Thr Asn Thr Met Ala Thr Gly Ser Gly
195 200 205
Ala Pro Met Ala Asp Asn Asn Glu Gly Ala Asp Gly Val Gly Asn Ser
210 215 220
Ser Gly Asn Trp His Cys Asp Ser Thr Trp Met Gly Asp Arg Val Ile
225 230 235 240
Thr Thr Ser Thr Arg Thr Trp Ala Leu Pro Thr Tyr Asn Asn His Leu
245 250 255
Tyr Lys Gln Ile Ser Ser Gln Ser Gly Ala Ser Asn Asp Asn His Tyr
260 265 270
Phe Gly Tyr Ser Thr Pro Trp Gly Tyr Phe Asp Phe Asn Arg Phe His
275 280 285
Cys His Phe Ser Pro Arg Asp Trp Gln Arg Leu Ile Asn Asn Asn Trp
290 295 300
Gly Phe Arg Pro Lys Arg Leu Asn Phe Lys Leu Phe Asn Ile Gln Val
305 310 315 320
Lys Glu Val Thr Gln Asn Asp Gly Thr Thr Thr Ile Ala Asn Asn Leu
325 330 335
Thr Ser Thr Val Gln Val Phe Thr Asp Ser Glu Tyr Gln Leu Pro Tyr
340 345 350
Val Leu Gly Ser Ala His Gln Gly Cys Leu Pro Pro Phe Pro Ala Asp
355 360 365
Val Phe Met Val Pro Gln Tyr Gly Tyr Leu Thr Leu Asn Asn Gly Ser
370 375 380
Gln Ala Val Gly Arg Ser Ser Phe Tyr Cys Leu Glu Tyr Phe Pro Ser
385 390 395 400
Gln Met Leu Arg Thr Gly Asn Asn Phe Thr Phe Ser Tyr Thr Phe Glu
405 410 415
Asp Val Pro Phe His Ser Gly Tyr Ala His Ser Gln Ser Leu Asp Arg
420 425 430
Leu Met Asn Pro Leu Ile Asp Gln Tyr Leu Tyr Tyr Leu Ser Thr Thr
435 440 445
Asn Thr Pro Ser Gly Thr Thr Thr Gln Ser Arg Leu Gln Phe Ser Gln
450 455 460
Ala Gly Ala Ser Asp Ile Arg Asp Gln Ser Arg Asn Trp Leu Pro Gly
465 470 475 480
Pro Cys Tyr Arg Gln Gln Arg Val Ser Lys Thr Ser Ala Asp Asn Asn
485 490 495
Asn Ser Glu Tyr Ser Trp Thr Gly Ala Thr Lys Tyr His Leu Asn Gly
500 505 510
Arg Asp Ser Leu Val Asn Pro Gly Pro Ala Met Ala Ser His Lys Asp
515 520 525
Asn Glu Glu Lys Phe Phe Pro Gln Ser Gly Val Leu Ile Phe Gly Lys
530 535 540
Gln Gly Ser Glu Lys Thr Asn Val Asp Ile Glu Lys Val Met Ile Thr
545 550 555 560
Asp Glu Glu Glu Ile Arg Thr Thr Asn Pro Val Ala Thr Glu Gln Tyr
565 570 575
Gly Ser Val Ser Thr Asn Leu Gln Arg Gly Asn Arg Gln Ala Ala Thr
580 585 590
Ala Asp Val Asn Thr Gln Gly Val Leu Pro Gly Met Val Trp Gln Asp
595 600 605
Arg Asp Val Tyr Leu Gln Gly Pro Ile Trp Ala Lys Ile Pro His Thr
610 615 620
Asp Gly His Phe His Pro Ser Pro Leu Met Gly Gly Phe Gly Leu Lys
625 630 635 640
His Pro Pro Pro Gln Ile Leu Ile Lys Asn Thr Pro Val Pro Ala Asn
645 650 655
Pro Ser Thr Thr Phe Ser Ala Ala Lys Phe Ala Ser Phe Ile Thr Gln
660 665 670
Tyr Ser Thr Gly Gln Val Ser Val Glu Ile Glu Trp Glu Leu Gln Lys
675 680 685
Glu Asn Ser Lys Arg Trp Asn Pro Glu Ile Gln Tyr Thr Ser Asn Tyr
690 695 700
Asn Lys Ser Val Asn Val Asp Phe Thr Val Asp Thr Asn Gly Val Tyr
705 710 715 720
Ser Glu Pro Arg Pro Ile Gly Thr Arg Tyr Leu Thr Arg Asn Leu
725 730 735
<210>191
<211>735
<212>PRT
<213>vp1,克隆hu.52
<400>191
Met Ala Ala Asp Gly Tyr Leu Pro Asp Trp Leu Glu Asp Thr Leu Ser
1 5 10 15
Glu Gly Ile Arg Gln Trp Trp Lys Leu Lys Pro Gly Pro Pro Pro Pro
20 25 30
Lys Pro Ala Glu Arg His Lys Asp Asp Ser Arg Gly Leu Val Leu Pro
35 40 45
Gly Tyr Lys Tyr Leu Gly Pro Phe Asn Gly Leu Asp Lys Gly Glu Pro
50 55 60
Val Asn Glu Ala Asp Ala Ala Ala Leu Glu His Asp Lys Ala Tyr Asp
65 70 75 80
Arg Gln Leu Asp Ser Gly Asp Asn Pro Tyr Leu Lys Tyr Asn His Ala
85 90 95
Asp Ala Glu Phe Gln Glu Arg Leu Lys Glu Asp Thr Ser Phe Gly Gly
100 105 110
Asn Leu Gly Arg Ala Val Phe Gln Ala Lys Lys Arg Val Leu Glu Pro
115 120 125
Leu Gly Leu Val Gly Glu Pro Val Lys Thr Ala Pro Gly Lys Lys Arg
130 135 140
Pro Val Glu His Ser Pro Val Glu Pro Asp Ser Ser Ser Gly Thr Gly
145 150 155 160
Lys Ala Gly Gln Gln Pro Ala Arg Lys Arg Leu Asn Phe Gly Gln Thr
165 170 175
Gly Asp Ala Asp Ser Val Pro Asp Pro Gln Pro Leu Gly Gln Pro Pro
180 185 190
Ala Ala Pro Ser Gly Leu Gly Thr Asn Thr Met Ala Thr Gly Ser Gly
195 200 205
Ala Pro Met Ala Asp Asn Asn Glu Gly Ala Asp Gly Val Gly Asn Ser
210 215 220
Ser Gly Asn Arg His Cys Asp Ser Thr Trp Met Gly Asp Arg Val Ile
225 230 235 240
Thr Thr Ser Thr Arg Thr Trp Ala Leu Pro Thr Tyr Asn Asn His Leu
245 250 255
Tyr Arg Gln Ile Ser Ser Gln Ser Gly Ala Ser Asn Asp Asn His Tyr
260 265 270
Phe Gly Tyr Ser Thr Pro Trp Gly Tyr Phe Asp Phe Asn Arg Phe His
275 280 285
Cys His Phe Ser Pro Arg Asp Trp Gln Arg Leu Ile Asn Asn Asn Trp
290 295 300
Gly Phe Arg Pro Lys Arg Leu Asn Phe Lys Leu Phe Asn Ile Gln Val
305 310 315 320
Lys Glu Val Thr Gln Asn Asp Gly Thr Thr Thr Ile Ala Asn Asn Leu
325 330 335
Thr Ser Thr Val Gln Val Phe Thr Asp Ser Glu Tyr Gln Leu Pro Tyr
340 345 350
Val Leu Gly Ser Ala His Gln Gly Cys Leu Pro Pro Phe Pro Ala Asp
355 360 365
Val Phe Met Val Pro Gln Tyr Gly Tyr Leu Thr Leu Ser Asn Gly Ser
370 375 380
Gln Ala Val Gly Arg Ser Ser Phe Tyr Cys Pro Glu Tyr Phe Pro Ser
385 390 395 400
Gln Met Leu Arg Thr Gly Asn Asn Phe Thr Phe Ser Tyr Thr Phe Glu
405 410 415
Asp Val Pro Phe His Ser Ser Tyr Ala His Ser Gln Ser Leu Asp Arg
420 425 430
Leu Met Asn Pro Leu Ile Asp Gln Tyr Leu Tyr Tyr Leu Ser Thr Thr
435 440 445
Asn Thr Pro Ser Gly Thr Thr Thr Gln Ser Arg Leu Gln Phe Ser Gln
450 455 460
Ala Gly Ala Ser Asp Ile Arg Asp Gln Ser Arg Asn Trp Leu Pro Gly
465 470 475 480
Pro Cys Tyr Arg Gln Gln Arg Val Ser Lys Thr Ser Ala Asp Asn Asn
485 490 495
Asn Ser Glu Tyr Ser Trp Thr Gly Ala Thr Lys Tyr His Leu Asn Gly
500 505 510
Arg Asp Ser Leu Val Asn Pro Gly Pro Ala Met Ala Ser His Lys Asp
515 520 525
Asn Glu Glu Lys Phe Phe Pro Gln Ser Gly Val Leu Ile Phe Gly Lys
530 535 540
Gln Gly Ser Glu Lys Thr Asn Val Asp Ile Glu Lys Val Met Ile Thr
545 550 555 560
Asp Glu Glu Glu Ile Arg Thr Thr Asn Pro Val Ala Thr Glu Gln Tyr
565 570 575
Gly Ser Val Ser Thr Asn Leu Gln Arg Gly Asn Arg Gln Ala Ala Thr
580 585 590
Ala Asp Val Asn Thr Gln Gly Val Leu Pro Gly Met Val Trp Gln Asp
595 600 605
Arg Asp Val Tyr Leu Gln Gly Pro Ile Trp Ala Lys Ile Pro His Thr
610 615 620
Asp Gly His Phe His Pro Ser Pro Leu Met Gly Gly Phe Gly Pro Lys
625 630 635 640
His Pro Pro Pro Gln Ile Leu Ile Lys Asn Thr Pro Val Pro Ala Asn
645 650 655
Pro Ser Thr Thr Phe Ser Ala Ala Lys Phe Ala Ser Phe Ile Thr Gln
660 665 670
Tyr Ser Thr Gly Gln Val Ser Val Glu Ile Glu Trp Glu Leu Gln Lys
675 680 685
Glu Asn Ser Lys Arg Trp Asn Pro Glu Ile Gln Tyr Thr Ser Asn Tyr
690 695 700
Asn Lys Ser Val Asn Val Asp Phe Thr Val Asp Thr Asn Gly Val Tyr
705 710 715 720
Ser Glu Pro Arg Pro Ile Gly Thr Arg Tyr Leu Thr Arg Asn Leu
725 730 735
<210>192
<211>735
<212>PRT
<213>vp1,克隆hu.56
<400>192
Met Ala Ala Asp Gly Tyr Leu Pro Asp Trp Leu Glu Asp Thr Leu Ser
1 5 10 15
Glu Gly Ile Arg Gln Trp Trp Lys Leu Lys Pro Gly Pro Pro Pro Pro
20 25 30
Lys Pro Ala Glu Arg His Lys Asp Asp Ser Arg Gly Leu Val Leu Pro
35 40 45
Gly Tyr Lys Tyr Leu Gly Pro Phe Asn Gly Leu Asp Lys Gly Glu Pro
50 55 60
Val Asn Glu Ala Asp Ala Ala Ala Leu Glu His Asp Lys Ala Tyr Asp
65 70 75 80
Arg Gln Leu Asp Ser Gly Asp Asn Pro Tyr Leu Lys Tyr Asn His Ala
85 90 95
Asp Ala Glu Phe Gln Glu Arg Leu Lys Glu Asp Thr Ser Phe Gly Gly
100 105 110
Asn Leu Gly Arg Ala Val Phe Gln Ala Lys Lys Arg Val Leu Glu Pro
115 120 125
Leu Gly Leu Val Glu Glu Pro Val Lys Thr Ala Pro Gly Lys Lys Arg
130 135 140
Pro Val Glu His Ser Pro Val Glu Pro Asp Ser Ser Ser Gly Thr Gly
145 150 155 160
Lys Ala Gly Asn Gln Pro Ala Arg Lys Arg Leu Asn Phe Gly Gln Thr
165 170 175
Gly Asp Ala Asp Ser Val Pro Asp Pro Gln Pro Leu Gly Gln Pro Pro
180 185 190
Ala Ser Pro Ser Gly Leu Gly Thr Asn Thr Met Ala Thr Gly Ser Gly
195 200 205
Ala Pro Met Ala Asp Asn Asn Glu Gly Ala Asp Gly Val Gly Asn Ser
210 215 220
Ser Gly Asn Trp His Cys Asp Ser Thr Trp Met Gly Asp Arg Val Val
225 230 235 240
Thr Thr Ser Thr Arg Thr Trp Ala Leu Pro Thr Tyr Asn Asn His Leu
245 250 255
Tyr Lys Gln Ile Ser Ser Gln Ser Gly Ala Ser Asn Asp Asn His Tyr
260 265 270
Phe Gly Tyr Ser Thr Pro Trp Gly Tyr Phe Asp Phe Asn Arg Phe His
275 280 285
Cys His Phe Ser Pro Arg Asp Trp Gln Arg Leu Ile Asn Asn Asn Trp
290 295 300
Gly Phe Arg Pro Lys Arg Leu Asn Phe Lys Leu Phe Asn Ile Gln Val
305 310 315 320
Lys Glu Val Thr Gln Asn Asp Gly Thr Thr Thr Ile Ala Asn Asn Leu
325 330 335
Thr Ser Thr Val Gln Val Phe Thr Asp Leu Glu Tyr Gln Leu Pro Tyr
340 345 350
Val Leu Gly Ser Ala His Gln Gly Cys Leu Pro Pro Phe Pro Ala Asp
355 360 365
Val Phe Met Val Pro Gln Tyr Gly Tyr Leu Thr Leu Asn Asn Gly Ser
370 375 380
Gln Ala Val Gly Arg Ser Ser Phe Tyr Cys Leu Glu Tyr Phe Pro Ser
385 390 395 400
Gln Met Leu Arg Thr Gly Asn Asn Phe Thr Phe Ser Tyr Thr Phe Glu
405 410 415
Asp Val Pro Phe His Ser Ser Tyr Ala His Ser Gln Ser Leu Asp Arg
420 425 430
Leu Met Asn Pro Leu Ile Asp Gln Tyr Leu Tyr Tyr Leu Ser Arg Thr
435 440 445
Asn Thr Pro Ser Gly Thr Thr Thr Gln Ser Arg Leu Gln Phe Ser Gln
450 455 460
Ala Gly Ala Ser Asp Ile Arg Asp Gln Ser Arg Asn Trp Leu Pro Gly
465 470 475 480
Pro Cys Tyr Arg Gln Gln Arg Val Ser Lys Thr Ala Ala Asp Asn Asn
485 490 495
Asn Ser Glu Tyr Ser Trp Thr Gly Ala Thr Lys Tyr His Leu Asn Gly
500 505 510
Arg Asp Ser Leu Val Asn Pro Gly Pro Ala Met Ala Ser His Lys Asp
515 520 525
Asp Glu Glu Lys Phe Phe Pro Gln Ser Gly Val Leu Ile Phe Gly Lys
530 535 540
Gln Gly Ser Glu Lys Thr Asn Val Asp Ile Glu Lys Val Met Ile Thr
545 550 555 560
Asp Glu Glu Glu Ile Arg Thr Thr Asn Pro Val Ala Thr Glu Gln Tyr
565 570 575
Gly Ser Val Ser Thr Asn Leu Gln Ser Gly Asn Thr Gln Ala Ala Thr
580 585 590
Ser Asp Val Asn Thr Gln Gly Val Leu Pro Gly Met Val Trp Gln Asp
595 600 605
Arg Asp Val Tyr Leu Gln Gly Pro Ile Trp Ala Lys Ile Pro His Thr
610 615 620
Asp Gly His Phe His Pro Ser Pro Leu Met Gly Gly Phe Gly Leu Lys
625 630 635 640
His Pro Pro Pro Gln Ile Leu Ile Lys Asn Thr Pro Val Pro Ala Asn
645 650 655
Pro Ser Thr Thr Phe Ser Ala Ala Lys Phe Ala Ser Phe Ile Thr Gln
660 665 670
Tyr Ser Thr Gly Gln Val Ser Val Glu Ile Glu Trp Glu Leu Gln Lys
675 680 685
Glu Asn Ser Lys Arg Trp Asn Pro Glu Ile Gln Tyr Thr Ser Asn Tyr
690 695 700
Asn Lys Ser Val Asn Val Asp Phe Thr Val Asp Thr Asn Gly Val Tyr
705 710 715 720
Ser Glu Pro Arg Pro Ile Gly Thr Arg Tyr Leu Thr Arg Asn Leu
725 730 735
<210>193
<211>734
<212>PRT
<213>vp1,克隆hu.57
<400>193
Met Ala Ala Asp Gly Tyr Leu Pro Asp Trp Leu Glu Asp Thr Leu Ser
1 5 10 15
Glu Gly Ile Arg Gln Trp Trp Lys Leu Lys Pro Gly Pro Pro Pro Lys
20 25 30
Pro Ala Glu Arg His Lys Asp Asp Ser Arg Gly Leu Val Leu Pro Gly
35 40 45
Tyr Lys Tyr Leu Gly Pro Phe Asn Gly Leu Asp Lys Gly Glu Pro Val
50 55 60
Asn Glu Ala Asp Ala Ala Ala Leu Glu His Asp Lys Ala Tyr Asp Arg
65 70 75 80
Gln Leu Asp Ser Gly Asp Asn Pro Tyr Leu Lys Tyr Asn His Ala Asp
85 90 95
Ala Glu Phe Gln Glu Arg Leu Lys Glu Asp Thr Ser Phe Gly Gly Asn
100 105 110
Leu Gly Arg Ala Val Phe Gln Ala Lys Lys Arg Val Leu Glu Pro Leu
115 120 125
Gly Leu Val Glu Glu Pro Val Lys Thr Ala Pro Gly Lys Lys Arg Pro
130 135 140
Val Glu His Ser Pro Val Glu Pro Asp Ser Ser Ser Gly Thr Gly Lys
145 150 155 160
Ala Gly Asn Gln Pro Ala Arg Lys Arg Leu Asn Phe Gly Gln Thr Gly
165 170 175
Asp Ala Asp Ser Val Pro Asp Pro Gln Pro Leu Gly Gln Pro Pro Ala
180 185 190
Ala Pro Ser Gly Leu Gly Thr Asn Thr Met Ala Thr Gly Ser Gly Ala
195 200 205
Pro Met Ala Asp Asn Asn Glu Gly Ala Asp Gly Val Gly Asn Ser Ser
210 215 220
Gly Asp Trp His Cys Asp Ser Thr Trp Met Gly Asp Arg Val Ile Thr
225 230 235 240
Thr Ser Thr Arg Thr Trp Ala Leu Pro Thr Tyr Asn Asn His Leu Tyr
245 250 255
Lys Gln Ile Ser Ser Gln Ser Gly Ala Ser Asn Asp Asn His Tyr Phe
260 265 270
Gly Tyr Ser Thr Pro Trp Gly Tyr Phe Asp Phe Asn Arg Phe His Cys
275 280 285
His Phe Ser Pro Arg Asp Trp Gln Arg Leu Ile Asn Asn Asn Trp Gly
290 295 300
Phe Arg Pro Lys Arg Leu Asn Leu Lys Leu Phe Asn Ile Gln Val Lys
305 310 315 320
Glu Val Thr Gln Asn Asp Gly Thr Thr Thr Ile Ala Asn Asn Leu Thr
325 330 335
Ser Thr Val Gln Val Phe Thr Asp Leu Glu Tyr Gln Leu Pro Tyr Val
340 345 350
Leu Gly Ser Ala His Gln Gly Cys Leu Pro Pro Phe Pro Ala Asp Val
355 360 365
Phe Met Val Pro Gln Tyr Gly Tyr Leu Thr Leu Asn Asn Gly Ser Gln
370 375 380
Ala Val Gly Arg Ser Ser Phe Tyr Cys Leu Glu Tyr Phe Pro Ser Gln
385 390 395 400
Met Leu Arg Thr Gly Asn Asn Phe Thr Phe Ser Tyr Thr Phe Glu Asp
405 410 415
Val Pro Phe His Ser Ser Tyr Ala His Ser Gln Ser Leu Asp Arg Leu
420 425 430
Met Asn Pro Leu Ile Asp Gln Tyr Leu Tyr Tyr Leu Ser Arg Thr Asn
435 440 445
Thr Pro Ser Gly Thr Thr Thr Gln Ser Arg Leu Gln Phe Ser Gln Ala
450 455 460
Gly Ala Ser Asp Ile Arg Asp Gln Ser Arg Asn Trp Leu Pro Gly Pro
465 470 475 480
Cys Tyr Arg Gln Gln Arg Val Ser Lys Thr Ala Ala Asp Asn Asn Asn
485 490 495
Gly Glu Tyr Ser Trp Thr Gly Ala Thr Lys Tyr His Leu Asn Gly Arg
500 505 510
Asp Ser Leu Val Asn Pro Gly Pro Ala Met Ala Ser His Lys Asp Asp
515 520 525
Glu Glu Lys Phe Phe Pro Gln Ser Gly Val Leu Ile Phe Gly Lys Gln
530 535 540
Gly Ser Glu Lys Thr Asn Val Asp Ile Glu Lys Val Met Ile Thr Asp
545 550 555 560
Glu Glu Glu Ile Arg Thr Thr Asn Pro Val Ala Thr Glu Gln Tyr Gly
565 570 575
Ser Val Ser Thr Asn Leu Gln Ser Gly Asn Thr Arg Ala Ala Thr Ser
580 585 590
Asp Val Asn Thr Gln Gly Val Leu Pro Gly Met Val Trp Gln Asp Arg
595 600 605
Asp Val Tyr Leu Gln Gly Pro Ile Trp Ala Lys Ile Pro His Thr Asp
610 615 620
Gly His Phe His Pro Ser Pro Leu Met Gly Gly Phe Gly Leu Lys His
625 630 635 640
Pro Pro Pro Gln Ile Leu Ile Lys Asn Thr Pro Val Pro Ala Asn Pro
645 650 655
Ser Thr Thr Phe Ser Ala Ala Lys Phe Ala Ser Phe Ile Thr Gln Tyr
660 665 670
Ser Thr Gly Gln Val Ser Val Glu Ile Glu Trp Glu Leu Gln Lys Glu
675 680 685
Asn Ser Lys Arg Trp Asn Pro Glu Ile Gln Tyr Thr Ser Asn Tyr Asn
690 695 700
Lys Ser Val Asn Val Asp Phe Thr Val Asp Thr Asn Gly Val Tyr Ser
705 710 715 720
Glu Pro Arg Pro Ile Gly Thr Arg Tyr Leu Thr Arg Asn Leu
725 730
<210>194
<211>735
<212>PRT
<213>vp1,克隆hu.58
<400>194
Met Ala Ala Asp Gly Tyr Leu Pro Asp Trp Leu Glu Asp Thr Leu Ser
1 5 10 15
Glu Gly Ile Arg Gln Trp Trp Lys Leu Lys Pro Gly Pro Pro Pro Pro
20 25 30
Lys Pro Ala Glu Arg His Lys Asp Asp Ser Arg Gly Leu Val Leu Pro
35 40 45
Gly Tyr Lys Tyr Leu Gly Pro Phe Asn Gly Leu Asp Lys Gly Glu Pro
50 55 60
Val Asn Glu Ala Asp Ala Ala Ala Leu Glu His Asp Lys Ala Tyr Asp
65 70 75 80
Arg Gln Leu Asp Ser Gly Asp Asn Pro Tyr Leu Lys Tyr Asp His Ala
85 90 95
Asp Ala Glu Phe Gln Glu Arg Leu Lys Glu Asp Thr Ser Phe Gly Gly
100 105 110
Asn Leu Gly Arg Ala Val Phe Gln Ala Lys Lys Arg Val Leu Glu Pro
115 120 125
Leu Gly Leu Val Glu Glu Pro Val Lys Thr Ala Pro Gly Lys Lys Arg
130 135 140
Pro Val Glu His Ser Pro Val Glu Pro Asp Ser Ser Ser Gly Thr Gly
145 150 155 160
Lys Ala Gly Asn Gln Pro Ala Arg Lys Arg Leu Asn Phe Gly Gln Thr
165 170 175
Gly Asp Ala Asp Ser Val Pro Asp Pro Gln Pro Leu Gly Gln Pro Pro
180 185 190
Ala Ala Pro Ser Gly Leu Gly Thr Asn Thr Met Ala Thr Gly Ser Gly
195 200 205
Ala Pro Met Ala Asp Asn Asn Asp Gly Ala Asp Gly Val Gly Asn Ser
210 215 220
Ser Gly Asn Trp His Cys Asp Ser Thr Trp Met Gly Asp Arg Val Ile
225 230 235 240
Thr Thr Ser Thr Arg Thr Trp Ala Leu Pro Thr Tyr Asn Asn His Leu
245 250 255
Tyr Lys Gln Ile Ser Ser Gln Ser Gly Ala Ser Asn Asp Asn His Tyr
260 265 270
Phe Gly Tyr Ser Thr Pro Trp Gly Tyr Phe Asp Phe Asn Arg Phe His
275 280 285
Cys His Phe Ser Pro Arg Asp Trp Gln Arg Leu Ile Asn Asn Asn Trp
290 295 300
Gly Phe Arg Pro Lys Arg Leu Asn Phe Lys Leu Phe Asn Ile Gln Val
305 310 315 320
Arg Glu Val Thr Gln Asn Asp Gly Thr Thr Thr Ile Ala Asn Asn Leu
325 330 335
Thr Ser Thr Val Gln Val Phe Thr Asp Ser Glu Tyr Gln Leu Pro Tyr
340 345 350
Val Leu Gly Ser Ala His Gln Gly Cys Leu Pro Pro Phe Pro Ala Asp
355 360 365
Val Phe Met Val Pro Gln Tyr Gly Tyr Leu Thr Leu Asn Asn Gly Ser
370 375 380
Gln Ala Val Gly Arg Ser Ser Phe Tyr Cys Leu Glu Tyr Phe Pro Ser
385 390 395 400
Gln Met Leu Arg Thr Gly Asn Asn Phe Thr Phe Ser Tyr Thr Phe Glu
405 410 415
Asp Val Pro Phe His Ser Ser Tyr Ala His Ser Gln Ser Leu Asp Arg
420 425 430
Leu Met Asn Pro Leu Ile Asp Gln Tyr Leu Tyr Tyr Leu Ser Arg Thr
435 440 445
Asn Thr Pro Ser Gly Thr Thr Thr Gln Ser Arg Leu Gln Phe Ser Gln
450 455 460
Ala Gly Ala Ser Asp Ile Arg Asp Gln Ser Arg Asn Trp Leu Pro Gly
465 470 475 480
Pro Cys Tyr Arg Gln Gln Arg Val Ser Lys Thr Ser Ala Asp Asn Asn
485 490 495
Asn Ser Glu Tyr Ser Trp Ile Gly Ala Thr Lys Tyr His Leu Asn Gly
500 505 510
Arg Asp Ser Leu Val Asn Pro Gly Pro Ala Met Ala Ser His Lys Asp
515 520 525
Asp Glu Glu Lys Phe Phe Pro Gln Ser Gly Val Leu Ile Phe Gly Lys
530 535 540
Gln Gly Ser Glu Lys Thr Asn Val Asp Ile Glu Lys Val Met Ile Thr
545 550 555 560
Asp Glu Glu Glu Ile Arg Thr Thr Asn Pro Val Ala Thr Glu Gln Tyr
565 570 575
Gly Ser Val Ser Thr Asn Leu Gln Ser Gly Asn Thr Gln Ala Ser Thr
580 585 590
Ala Asp Val Asn Thr Gln Gly Val Leu Pro Gly Met Val Trp Gln Asp
595 600 605
Arg Asp Val Tyr Leu Gln Gly Pro Ile Trp Ala Lys Ile Pro His Thr
610 615 620
Asp Gly His Phe His Pro Ser Pro Leu Met Gly Gly Phe Gly Leu Lys
625 630 635 640
His Pro Pro Pro Gln Ile Leu Ile Lys Asn Thr Pro Val Pro Ala Asn
645 650 655
Pro Ser Thr Thr Phe Ser Ala Ala Lys Phe Ala Ser Phe Ile Thr Gln
660 665 670
Tyr Ser Thr Gly Arg Val Ser Val Glu Ile Glu Trp Glu Leu Gln Lys
675 680 685
Glu Asn Ser Lys Arg Trp Asn Pro Glu Ile Gln Tyr Thr Ser Asn Tyr
690 695 700
Asn Lys Ser Val Asn Val Asp Phe Thr Val Asp Thr Asn Gly Val Tyr
705 710 715 720
Ser Glu Pro Arg Pro Ile Gly Thr Arg Tyr Leu Thr Arg Asn Leu
725 730 735
<210>195
<211>735
<212>PRT
<213>vp1,克隆hu.63
<400>195
Met Ala Ala Asp Gly Tyr Leu Pro Asp Trp Leu Glu Asp Thr Leu Ser
1 5 10 15
Glu Gly Ile Arg Gln Trp Trp Lys Leu Lys Pro Gly Pro Pro Pro Pro
20 25 30
Lys Pro Ala Glu Arg His Lys Asp Asp Ser Arg Gly Leu Val Leu Pro
35 40 45
Gly Tyr Lys Tyr Leu Gly Pro Phe Asn Gly Leu Asp Lys Gly Glu Pro
50 55 60
Val Asn Glu Ala Asp Ala Ala Ala Leu Glu His Asp Lys Ala Tyr Asp
65 70 75 80
Arg Gln Leu Asp Ser Gly Asp Asn Pro Tyr Pro Lys Tyr Asn His Ala
85 90 95
Asp Ala Glu Phe Gln Glu Arg Leu Lys Glu Asp Thr Ser Phe Gly Gly
100 105 110
Asn Leu Gly Arg Ala Val Phe Gln Ala Lys Lys Arg Val Leu Glu Pro
115 120 125
Leu Gly Leu Val Glu Glu Pro Val Lys Thr Ala Pro Gly Lys Lys Arg
130 135 140
Pro Val Glu His Ser Pro Ala Glu Pro Asp Ser Ser Ser Gly Thr Gly
145 150 155 160
Lys Ala Gly Gln Gln Pro Ala Arg Lys Arg Leu Asn Phe Gly Gln Thr
165 170 175
Gly Asp Ala Asp Ser Val Pro Asp Pro Gln Pro Leu Gly Gln Pro Pro
180 185 190
Ala Ala Pro Ser Gly Leu Gly Thr Asn Thr Met Ala Thr Gly Ser Gly
195 200 205
Ala Pro Met Ala Asp Asn Asn Glu Gly Ala Asp Gly Val Gly Asn Ser
210 215 220
Ser Gly Asn Trp His Cys Asp Ser Thr Trp Met Gly Asp Arg Val Ile
225 230 235 240
Thr Thr Ser Thr Arg Thr Trp Ala Leu Pro Thr Tyr Asn Asn His Leu
245 250 255
Tyr Lys Gln Ile Ser Ser Gln Ser Gly Ala Ser Asn Asp Asn His Tyr
260 265 270
Phe Gly Tyr Ser Thr Pro Trp Gly Tyr Phe Asp Phe Asn Arg Phe His
275 280 285
Cys His Phe Ser Pro Arg Asp Trp Gln Arg Leu Ile Asn Asn Asn Trp
290 295 300
Gly Phe Arg Pro Lys Arg Leu Asn Phe Lys Leu Phe Asn Ile Gln Val
305 310 315 320
Lys Glu Val Thr Gln Asn Asp Gly Thr Thr Thr Ile Ala Asn Asn Leu
325 330 335
Thr Ser Thr Val Gln Val Phe Thr Asp Ser Glu Tyr Gln Leu Pro Tyr
340 345 350
Val Leu Gly Ser Ala His Gln Gly Cys Leu Pro Pro Phe Pro Ala Asp
355 360 365
Val Phe Met Val Pro Gln Tyr Gly Tyr Leu Thr Leu Asn Asn Gly Ser
370 375 380
Gln Ala Val Gly Arg Ser Ser Phe Tyr Cys Leu Glu Tyr Phe Pro Ser
385 390 395 400
Gln Met Leu Arg Thr Gly Asn Asn Phe Thr Phe Ser Tyr Thr Phe Glu
405 410 415
Asp Val Pro Phe His Ser Ser Tyr Ala His Ser Gln Ser Leu Asp Arg
420 425 430
Leu Met Asn Pro Leu Ile Asp Gln Tyr Leu Tyr Tyr Leu Ser Arg Thr
435 440 445
Asn Thr Pro Ser Gly Thr Thr Thr Gln Ser Arg Leu Gln Phe Ser Gln
450 455 460
Ala Gly Ala Ser Asp Ile Arg Asp Gln Ser Arg Asn Trp Leu Pro Gly
465 470 475 480
Pro Cys Tyr Arg Gln Gln Arg Val Ser Lys Thr Ser Ala Asp Asn Asn
485 490 495
Asn Ser Glu Tyr Ser Trp Thr Gly Ala Thr Lys Tyr His Leu Asn Gly
500 505 510
Arg Asp Ser Leu Val Asn Pro Gly Pro Ala Met Ala Ser His Lys Asp
515 520 525
Asp Glu Glu Lys Phe Phe Pro Gln Ser Gly Val Leu Ile Phe Gly Lys
530 535 540
Gln Asp Ser Gly Lys Thr Asn Val Asp Ile Glu Lys Val Met Ile Thr
545 550 555 560
Asp Glu Glu Glu Ile Arg Thr Thr Asn Pro Val Ala Thr Glu Gln Tyr
565 570 575
Gly Ser Val Ser Thr Asn Leu Gln Ser Gly Asn Thr Gln Ala Ala Thr
580 585 590
Ser Asp Val Asn Thr Gln Gly Val Leu Pro Gly Met Val Trp Gln Asp
595 600 605
Arg Asp Val Tyr Leu Gln Gly Pro Ile Trp Ala Lys Ile Pro His Thr
610 615 620
Asp Gly His Phe His Pro Ser Pro Leu Met Gly Gly Phe Gly Leu Lys
625 630 635 640
His Pro Pro Pro Gln Ile Leu Ile Lys Asn Thr Pro Val Pro Ala Asn
645 650 655
Pro Ser Thr Thr Phe Ser Ala Ala Lys Phe Ala Ser Phe Ile Thr Gln
660 665 670
Tyr Ser Thr Gly Gln Val Ser Val Glu Ile Glu Trp Glu Leu Gln Lys
675 680 685
Glu Asn Ser Lys Arg Trp Asn Pro Glu Ile Gln Tyr Thr Ser Asn Tyr
690 695 700
Asn Lys Ser Val Asn Val Asp Phe Thr Val Asp Thr Asn Gly Val Tyr
705 710 715 720
Ser Glu Pro Arg Pro Ile Gly Thr Arg Tyr Leu Thr Arg Asn Leu
725 730 735
<210>196
<211>735
<212>PRT
<213>vp1,克隆hu.64
<400>196
Met Ala Ala Asp Gly Tyr Leu Pro Asp Trp Leu Glu Asp Thr Leu Ser
1 5 10 15
Glu Gly Ile Arg Gln Trp Trp Lys Leu Lys Pro Gly Pro Pro Pro Pro
20 25 30
Lys Pro Ala Glu Arg His Lys Asp Asp Ser Arg Gly Leu Val Leu Pro
35 40 45
Gly Tyr Lys Tyr Leu Gly Pro Phe Asn Gly Leu Asp Lys Gly Glu Pro
50 55 60
Val Asn Glu Ala Asp Ala Ala Ala Leu Glu His Asp Lys Ala Tyr Asp
65 70 75 80
Arg Gln Leu Asp Gly Gly Asp Asn Pro Tyr Leu Lys Tyr Asn His Ala
85 90 95
Asp Ala Glu Phe Gln Glu Arg Leu Lys Glu Asp Thr Ser Phe Gly Gly
100 105 110
Asn Leu Gly Arg Ala Val Phe Gln Ala Lys Lys Arg Val Leu Glu Pro
115 120 125
Leu Gly Leu Val Glu Glu Pro Val Lys Thr Ala Pro Gly Lys Lys Arg
130 135 140
Pro Val Glu His Ser Leu Ala Glu Pro Asp Ser Ser Ser Gly Thr Gly
145 150 155 160
Lys Ala Gly Gln Gln Pro Ala Arg Arg Arg Leu Asn Phe Gly Gln Thr
165 170 175
Gly Asp Ala Asp Ser Val Pro Asp Pro Gln Pro Leu Gly Gln Pro Pro
180 185 190
Ala Ala Pro Ser Gly Leu Gly Thr Asn Thr Met Ala Thr Gly Ser Gly
195 200 205
Ala Pro Met Ala Asp Asn Asn Glu Gly Ala Asp Gly Val Gly Asn Ser
210 215 220
Ser Gly Asn Trp His Cys Asp Ser Thr Trp Met Gly Asp Arg Val Ile
225 230 235 240
Thr Thr Ser Thr Arg Thr Trp Ala Leu Pro Thr Tyr Asn Asn His Leu
245 250 255
Tyr Arg Gln Ile Ser Ser Gln Ser Gly Ala Ser Asn Asp Asn His Tyr
260 265 270
Phe Gly Tyr Ser Thr Pro Trp Gly Tyr Phe Asp Phe Asn Arg Phe His
275 280 285
Cys His Phe Ser Pro Arg Asp Trp Gln Arg Leu Ile Asn Asn Asn Trp
290 295 300
Gly Ser Arg Pro Lys Arg Leu Asn Phe Lys Leu Phe Asn Ile Gln Val
305 310 315 320
Lys Glu Val Thr Gln Asn Asp Gly Thr Thr Thr Ile Ala Asn Asn Leu
325 330 335
Thr Ser Thr Val Gln Val Phe Thr Asp Ser Glu Tyr Gln Leu Pro Tyr
340 345 350
Val Leu Gly Ser Ala His Gln Gly Cys Leu Pro Pro Phe Pro Ala Asp
355 360 365
Val Phe Met Val Pro Gln Tyr Gly Tyr Leu Thr Leu Asn Asn Gly Ser
370 375 380
Gln Ala Val Gly Arg Ser Ser Phe Tyr Cys Leu Glu Tyr Phe Pro Ser
385 390 395 400
Gln Met Leu Arg Thr Gly Asn Asn Phe Thr Phe Ser Tyr Thr Phe Glu
405 410 415
Asp Val Pro Phe His Ser Ser Tyr Ala His Ser Gln Ser Leu Asp Arg
420 425 430
Leu Met Asn Pro Leu Val Asp Gln Tyr Leu Tyr Tyr Leu Ser Arg Thr
435 440 445
Asn Thr Pro Ser Gly Thr Thr Thr Gln Ser Arg Leu Gln Phe Ser Gln
450 455 460
Ala Gly Ala Ser Asp Ile Arg Asp Gln Ser Arg Asn Trp Leu Pro Gly
465 470 475 480
Pro Cys Tyr Arg Gln Gln Arg Val Ser Lys Thr Ser Ala Asp Asn Asn
485 490 495
Asn Ser Glu Tyr Ser Trp Thr Gly Ala Thr Lys Tyr His Leu Asn Gly
500 505 510
Arg Asp Ser Leu Val Asn Pro Gly Pro Ala Met Ala Ser His Lys Asp
515 520 525
Asp Glu Glu Lys Phe Phe Pro Gln Ser Gly Val Leu Ile Phe Gly Lys
530 535 540
Gln Asp Ser Gly Lys Thr Asn Val Asp Ile Glu Lys Val Met Ile Thr
545 550 555 560
Asp Glu Glu Glu Ile Arg Thr Thr Asn Pro Val Ala Thr Glu Gln Tyr
565 570 575
Gly Ser Val Ser Thr Asn Leu Gln Ser Gly Asn Thr Gln Ala Ala Thr
580 585 590
Ser Asp Val Asn Thr Gln Gly Val Leu Pro Gly Met Val Trp Gln Asp
595 600 605
Arg Asp Val Tyr Leu Gln Gly Pro Ile Trp Ala Lys Ile Pro His Thr
610 615 620
Asp Gly His Phe His Pro Ser Pro Leu Met Gly Gly Phe Gly Leu Lys
625 630 635 640
His Pro Pro Pro Gln Ile Leu Ile Lys Asn Thr Pro Val Pro Ala Asn
645 650 655
Pro Ser Thr Thr Phe Ser Ala Ala Lys Phe Ala Ser Phe Ile Thr Gln
660 665 670
Tyr Ser Thr Gly Gln Val Ser Val Glu Ile Glu Trp Glu Leu Gln Lys
675 680 685
Glu Asn Ser Lys Arg Trp Asn Pro Glu Ile Gln Tyr Thr Ser Asn Tyr
690 695 700
Asn Lys Ser Val Asn Val Asp Phe Thr Val Asp Thr Asn Gly Val Tyr
705 710 715 720
Ser Glu Pro Arg Pro Ile Gly Thr Arg Tyr Leu Thr Arg Asn Leu
725 730 735
<210>197
<211>738
<212>PRT
<213>vp1,克隆hu.66
<400>197
Met Ala Ala Asp Gly Tyr Leu Pro Asp Trp Leu Glu Asp Asn Leu Ser
1 5 10 15
Glu Gly Ile Arg Glu Trp Trp Asp Leu Lys Pro Gly Ala Pro Lys Pro
20 25 30
Lys Ala Asn Gln Gln Lys Gln Asp Asp Gly Arg Gly Leu Val Leu Pro
35 40 45
Gly Tyr Lys Tyr Leu Gly Pro Phe Asn Gly Leu Asp Lys Gly Glu Pro
50 55 60
Val Asn Ala Ala Asp Ala Ala Ala Leu Glu His Asp Lys Ala Tyr Asp
65 70 75 80
Gln Gln Leu Lys Ala Gly Asp Asn Pro Tyr Leu Arg Tyr Asn His Ala
85 90 95
Asp Ala Glu Phe Gln Glu Arg Leu Gln Glu Asp Thr Ser Phe Gly Gly
100 105 110
Asn Leu Gly Arg Ala Val Phe Gln Ala Lys Lys Arg Val Leu Glu Pro
115 120 125
Leu Gly Leu Val Glu Glu Ala Ala Lys Thr Ala Pro Gly Lys Lys Arg
130 135 140
Pro Val Glu Pro Ser Pro Gln Arg Ser Pro Asp Ser Ser Ala Gly Ile
145 150 155 160
Gly Lys Lys Gly Gln Gln Pro Ala Lys Lys Arg Leu Asn Phe Gly Gln
165 170 175
Thr Gly Asp Ser Glu Ser Val Pro Asp Pro Gln Pro Ile Gly Glu Pro
180 185 190
Pro Ala Gly Pro Ser Gly Leu Gly Ser Gly Thr Met Ala Ala Gly Gly
195 200 205
Gly Ala Pro Met Ala Asp Asn Asn Glu Gly Ala Asp Gly Val Gly Ser
210 215 220
Ser Ser Gly Asn Trp His Cys Asp Ser Thr Trp Leu Gly Asp Arg Val
225 230 235 240
Ile Thr Thr Ser Thr Arg Thr Trp Ala Leu Pro Thr Tyr Asn Asn His
245 250 255
Leu Tyr Lys Gln Ile Ser Asn Gly Thr Ser Gly Gly Ser Thr Asn Asp
260 265 270
Asn Thr Tyr Phe Gly Tyr Ser Thr Pro Trp Gly Tyr Phe Asp Phe Asn
275 280 285
Arg Phe His Cys His Phe Ser Pro Arg Asp Trp Gln Arg Leu Ile Asn
290 295 300
Asn Asn Trp Gly Phe Arg Pro Lys Arg Leu Ser Phe Lys Leu Phe Asn
305 310 315 320
Ile Gln Val Lys Glu Val Thr Gln Asn Glu Gly Thr Glu Thr Ile Ala
325 330 335
Asn Asn Leu Thr Ser Thr Ile Gln Val Phe Thr Asp Ser Glu Tyr Gln
340 345 350
Leu Pro Tyr Val Leu Gly Ser Ala His Gln Gly Cys Leu Pro Pro Phe
355 360 365
Pro Ala Asp Val Phe Met Ile Pro Gln Tyr Gly Tyr Leu Thr Leu Asn
370 375 380
Asn Gly Ser Gln Ala Val Gly Arg Ser Ser Phe Tyr Cys Leu Glu Tyr
385 390 395 400
Phe Pro Ser Gln Met Leu Arg Thr Gly Asn Asn Phe Glu Phe Ser Tyr
405 410 415
Thr Phe Glu Asp Val Pro Phe His Ser Ser Cys Ala His Ser Gln Ser
420 425 430
Ser Asp Arg Leu Met Asn Pro Leu Ile Asp Gln Tyr Leu Tyr Tyr Leu
435 440 445
Ser Arg Thr Arg Ser Thr Gly Gly Thr Gln Gly Thr Gln Gln Leu Leu
450 455 460
Phe Ser Gln Ala Gly Pro Ala Asn Met Ser Ala Gln Ala Lys Asn Trp
465 470 475 480
Leu Pro Gly Pro Cys Tyr Arg Gln Gln Arg Val Ser Thr Thr Leu Ser
485 490 495
Gln Asn Asn Asn Ser Asn Phe Ala Trp Thr Gly Ala Thr Lys Tyr His
500 505 510
Leu Asn Gly Arg Asp Ser Leu Val Asn Pro Gly Val Ala Met Ala Thr
515 520 525
His Lys Asp Asp Glu Glu Arg Phe Phe Pro Ser Ser Gly Val Leu Met
530 535 540
Phe Gly Lys Gln Gly Ala Gly Arg Asp Asn Val Asp Tyr Ser Ser Val
545 550 555 560
Met Leu Thr Ser Glu Glu Glu Ile Lys Thr Thr Asn Pro Val Ala Thr
565 570 575
Glu Gln Tyr Gly Val Val Ala Asp Asn Leu Gln Gln Thr Asn Thr Gly
580 585 590
Pro Ile Val Gly Asn Val Asn Ser Gln Gly Ala Leu Pro Gly Met Val
595 600 605
Trp Gln Asn Arg Asp Val Tyr Leu Gln Gly Pro Ile Trp Ala Lys Ile
610 615 620
Pro His Thr Asp Gly Asn Phe His Pro Ser Pro Leu Met Gly Gly Phe
625 630 635 640
Gly Leu Lys His Pro Pro Pro Gln Ile Leu Ile Lys Asn Thr Pro Val
645 650 655
Pro Ala Asp Pro Pro Thr Thr Phe Ser Gln Ala Lys Leu Ala Ser Phe
660 665 670
Ile Thr Gln Tyr Set Thr Gly Gln Val Ser Val Glu Ile Glu Trp Glu
675 680 685
Leu Gln Lys Glu Asn Ser Lys Arg Trp Asn Pro Glu Ile Gln Tyr Thr
690 695 700
Ser Asn Tyr Tyr Lys Ser Thr Asn Val Asp Phe Ala Val Asn Thr Glu
705 710 715 720
Gly Thr Tyr Ser Glu Pro Arg Pro Ile Gly Thr Arg Tyr Leu Thr Arg
725 730 735
Asn Leu
<210>198
<211>738
<212>PRT
<213>vp1,克隆hu.67
Met Ala Ala Asp Gly Tyr Leu Pro Asp Trp Leu Glu Asp Asn Leu Ser
1 5 10 15
Glu Gly Ile Arg Glu Trp Trp Asp Leu Lys Pro Gly Ala Pro Lys Pro
20 25 30
Lys Ala Asn Gln Gln Lys Gln Asp Asp Gly Arg Gly Leu Val Leu Leu
35 40 45
Gly Tyr Lys Tyr Leu Gly Pro Phe Asn Gly Leu Asp Lys Gly Glu Pro
50 55 60
Val Asn Ala Ala Asp Ala Ala Ala Leu Glu His Asp Lys Ala Tyr Asp
65 70 75 80
Gln Gln Leu Lys Ala Gly Asp Asn Pro Tyr Leu Arg Tyr Asn His Ala
85 90 95
Asp Ala Glu Phe Gln Glu Arg Leu Gln Glu Asp Thr Ser Phe Gly Gly
100 105 110
Asn Leu Gly Arg Ala Val Phe Gln Ala Lys Lys Arg Val Leu Glu Pro
115 120 125
Leu Gly Leu Val Glu Glu Ala Ala Lys Thr Ala Pro Gly Lys Lys Arg
130 135 140
Pro Val Glu Pro Ser Pro Gln Arg Ser Pro Asp Ser Ser Thr Gly Ile
145 150 155 160
Gly Lys Lys Gly Gln Gln Pro Ala Lys Lys Arg Leu Asn Phe Gly Gln
165 170 175
Thr Gly Asp Ser Glu Ser Val Pro Asp Pro Gln Pro Ile Gly Glu Pro
180 185 190
Pro Ala Gly Pro Ser Gly Leu Gly Ser Gly Thr Met Ala Ala Gly Gly
195 200 205
Gly Ala Pro Met Ala Asp Asn Asn Glu Gly Ala Asp Gly Val Gly Ser
210 215 220
Ser Ser Gly Asn Trp His Cys Asp Ser Thr Trp Leu Gly Asp Arg Val
225 230 235 240
Ile Thr Thr Ser Thr Arg Thr Trp Ala Leu Pro Thr Tyr Asn Asn His
245 250 255
Leu Tyr Lys Gln Ile Ser Asn Gly Thr Ser Gly Gly Ser Thr Asn Asp
260 265 270
Asn Thr Tyr Phe Gly Tyr Ser Thr Pro Trp Gly Tyr Phe Asp Phe Asn
275 280 285
Arg Phe His Cys His Phe Ser Pro Arg Asp Trp Gln Arg Leu Ile Asn
290 295 300
Asn Asn Trp Gly Phe Arg Pro Lys Arg Leu Ser Phe Lys Leu Phe Asn
305 310 315 320
Ile Gln Val Lys Glu Val Thr Gln Asn Glu Gly Thr Lys Thr Ile Ala
325 330 335
Asn Asn Leu Thr Ser Thr Ile Gln Val Phe Thr Asp Ser Glu Tyr Gln
340 345 350
Leu Pro Tyr Val Leu Gly Ser Ala His Gln Gly Cys Leu Pro Pro Phe
355 360 365
Pro Ala Asp Val Phe Met Ile Pro Gln Tyr Gly Tyr Leu Thr Leu Asn
370 375 380
Asn Gly Ser Gln Ala Val Gly Arg Ser Ser Phe Tyr Cys Leu Glu Tyr
385 390 395 400
Phe Pro Ser Gln Met Leu Arg Thr Gly Asn Asn Phe Glu Phe Ser Tyr
405 410 415
Thr Phe Glu Asp Val Pro Phe His Ser Gly Tyr Ala His Ser Gln Ser
420 425 430
Leu Asp Arg Leu Met Asn Pro Leu Ile Asp Gln Tyr Leu Tyr Tyr Leu
435 440 445
Ser Arg Thr Gln Ser Thr Gly Gly Thr Gln Gly Thr Gln Gln Leu Leu
450 455 460
Phe Ser Gln Ala Gly Pro Ala Asn Met Ser Ala Gln Ala Lys Asn Trp
465 470 475 480
Leu Pro Gly Pro Cys Tyr Arg Gln Gln Arg Val Ser Thr Thr Leu Ser
485 490 495
Gln Asn Asn Asn Ser Asn Phe Ala Trp Thr Gly Ala Thr Lys Tyr His
500 505 510
Leu Asn Gly Arg Asp Ser Leu Val Asn Pro Gly Val Ala Met Ala Thr
515 520 525
His Lys Asp Asp Glu Glu Arg Phe Phe Pro Ser Ser Gly Val Leu Met
530 535 540
Phe Gly Lys Gln Gly Ala Gly Arg Asp Asn Val Asp Tyr Ser Ser Val
545 550 555 560
Met Leu Thr Ser Glu Glu Glu Ile Lys Thr Thr Asn Pro Val Ala Thr
565 570 575
Glu Gln Tyr Gly Val Val Ala Asp Asn Leu Gln Gln Thr Asn Thr Gly
580 585 590
Pro Ile Val Gly Asn Val Asn Ser Gln Gly Ala Leu Pro Gly Met Val
595 600 605
Trp Gln Asn Arg Asp Val Tyr Leu Gln Gly Pro Ile Trp Ala Lys Ile
610 615 620
Pro His Thr Asp Gly Asn Phe His Pro Ser Pro Leu Met Gly Gly Phe
625 630 635 640
Gly Leu Lys His Pro Pro Pro Gln Ile Leu Ile Lys Asn Thr Pro Val
645 650 655
Pro Ala Asp Pro Pro Thr Thr Phe Ser Gln Ala Lys Leu Ala Ser Phe
660 665 670
Ile Thr Gln Tyr Ser Thr Gly Gln Val Ser Val Glu Ile Glu Trp Glu
675 680 685
Leu Gln Lys Glu Asn Ser Lys Arg Trp Asn Pro Glu Ile Gln Tyr Thr
690 695 700
Ser Asn Tyr Tyr Lys Ser Thr Asn Val Asp Phe Ala Val Asn Thr Glu
705 710 715 720
Gly Thr Tyr Ser Glu Pro Arg Pro Ile Gly Thr Arg Tyr Leu Thr Arg
725 730 735
Asn Leu
<210>199
<211>2175
<212>DNA
<213>腺伴随病毒血清型5
<400>199
atgtcttttg ttgatcaccc tccagattgg ttggaagaag ttggtgaagg tcttcgcgag 60
tttttgggcc ttgaagcggg cccaccgaaa ccaaaaccca atcagcagca tcaagatcaa 120
gcccgtggtc ttgtgctgcc tggttataac tatctcggac ccggaaacgg tctcgatcga 180
ggagagcctg tcaacagggc agacgaggtc gcgcgagagc acgacatctc gtacaacgag 240
cagcttgagg cgggagacaa cccctacctc aagtacaacc acgcggacgc cgagtttcag 300
gagaagctcg ccgacgacac atccttcggg ggaaacctcg gaaaggcagt ctttcaggcc 360
aagaaaaggg ttctcgaacc ttttggcctg gttgaagagg gtgctaagac ggcccctacc 420
ggaaagcgga tagacgacca ctttccaaaa agaaagaagg ctcggaccga agaggactcc 480
aagccttcca cctcgtcaga cgccgaagct ggacccagcg gatcccagca gctgcaaatc 540
ccagcccaac cagcctcaag tttgggagct gatacaatgt ctgcgggagg tggcggccca 600
ttgggcgaca ataaccaagg tgccgatgga gtgggcaatg cctcgggaga ttggcattgc 660
gattccacgt ggatggggga cagagtcgtc accaagtcca cccgaacctg ggtgctgccc 720
agctacaaca accaccagta ccgagagatc aaaagcggct ccgtcgacgg aagcaacgcc 780
aacgcctact ttggatacag caccccctgg gggtactttg actttaaccg cttccacagc 840
cactggagcc cccgagactg gcaaagactc atcaacaact actggggctt cagaccccgg 900
tccctcagag tcaaaatctt caacattcaa gtcaaagagg tcacggtgca ggactccacc 960
accaccatcg ccaacaacct cacctccacc gtccaagtgt ttacggacga cgactaccag 1020
ctgccctacg tcgtcggcaa cgggaccgag ggatgcctgc cggccttccc tccgcaggtc 1080
tttacgctgc cgcagtacgg ttacgcgacg ctgaaccgcg acaacacaga aaatcccacc 1140
gagaggagca gcttcttctg cctagagtac tttcccagca agatgctgag aacgggcaac 1200
aactttgagt ttacctacaa ctttgaggag gtgcccttcc actccagctt cgctcccagt 1260
cagaacctgt tcaagctggc caacccgctg gtggaccagt acttgtaccg cttcgtgagc 1320
acaaataaca ctggcggagt ccagttcaac aagaacctgg ccgggagata cgccaacacc 1380
tacaaaaact ggttcccggg gcccatgggc cgaacccagg gctggaacct gggctccggg 1440
gtcaaccgcg ccagtgtcag cgccttcgcc acgaccaata ggatggagct cgagggcgcg 1500
agttaccagg tgcccccgca gccgaacggc atgaccaaca acctccaggg cagcaacacc 1560
tatgccctgg agaacactat gatcttcaac agccagccgg cgaacccggg caccaccgcc 1620
acgtacctcg agggcaacat gctcatcacc agcgagagcg agacgcagcc ggtgaaccgc 1680
gtggcgtaca acgtcggcgg gcagatggcc accaacaacc agagctccac cactgccccc 1740
gcgaccggca cgtacaacct ccaggaaatc gtgcccggca gcgtgtggat ggagagggac 1800
gtgtacctcc aaggacccat ctgggccaag atcccagaga cgggggcgca ctttcacccc 1860
tctccggcca tgggcggatt cggactcaaa cacccaccgc ccatgatgct catcaagaac 1920
acgcctgtgc ccggaaatat caccagcttc tcggacgtgc ccgtcagcag cttcatcacc 1980
cagtacagca ccgggcaggt caccgtggag atggagtggg agctcaagaa ggaaaactcc 2040
aagaggtgga acccagagat ccagtacaca aacaactaca acgaccccca gtttgtggac 2100
tttgccccgg acagcaccgg ggaatacaga accaccagac ctatcggaac ccgatacctt 2160
acccgacccc tttaa 2175
<210>200
<211>2211
<212>DNA
<213>腺伴随病毒,血清型3-3
<400>200
atggctgctg acggttatct tccagattgg ctcgaggaca acctttctga aggcattcgt 60
gagtggtggg ctctgaaacc tggagtccct caacccaaag cgaaccaaca acaccaggac 120
aaccgtcggg gtcttgtgct tccgggttac aaatacctcg gacccggtaa cggactcgac 180
aaaggagagc cggtcaacga ggcggacgcg gcagccctcg aacacgacaa agcttacgac 240
cagcagctca aggccggtga caacccgtac ctcaagtaca accacgccga cgccgagttt 300
caggagcgtc ttcaagaaga tacgtctttt gggggcaacc ttggcagagc agtcttccag 360
gccaaaaaga ggatccttga gcctcttggt ctggttgagg aagcagctaa aacggctcct 420
ggaaagaagg gggctgtaga tcagtctcct caggaaccgg actcatcatc tggtgttggc 480
aaatcgggca aacagcctgc cagaaaaaga ctaaatttcg gtcagactgg agactcagag 540
tcagtcccag accctcaacc tctcggagaa ccaccagcag cccccacaag tttgggatct 600
aatacaatgg cttcaggcgg tggcgcacca atggcagaca ataacgaggg tgccgatgga 660
gtgggtaatt cctcaggaaa ttggcattgc gattcccaat ggctgggcga cagagtcatc 720
accaccagca ccagaacctg ggccctgccc acttacaaca accatctcta caagcaaatc 780
tccagccaat caggagcttc aaacgacaac cactactttg gctacagcac cccttggggg 840
tattttgact ttaacagatt ccactgccac ttctcaccac gtgactggca gcgactcatt 900
aacaacaact ggggattccg gcccaagaaa ctcagcttca agctcttcaa catccaagtt 960
agaggggtca cgcagaacga tggcacgacg actattgcca ataaccttac cagcacggtt 1020
caagtgttta cggactcgga gtatcagctc ccgtacgtgc tcgggtcggc gcaccaaggc 1080
tgtctcccgc cgtttccagc ggacgtcttc atggtccctc agtatggata cctcaccctg 1140
aacaacggaa gtcaagcggt gggacgctca tccttttact gcctggagta cttcccttcg 1200
cagatgctaa ggactggaaa taacttccaa ttcagctata ccttcgagga tgtacctttt 1260
cacagcagct acgctcacag ccagagtttg gatcgcttga tgaatcctct tattgatcag 1320
tatctgtact acctgaacag aacgcaagga acaacctctg gaacaaccaa ccaatcacgg 1380
ctgcttttta gccaggctgg gcctcagtct atgtctttgc aggccagaaa ttggctacct 1440
gggccctgct accggcaaca gagactttca aagactgcta acgacaacaa caacagtaac 1500
tttccttgga cagcggccag caaatatcat ctcaatggcc gcgactcgct ggtgaatcca 1560
ggaccagcta tggccagtca caaggacgat gaagaaaaat ttttccctat gcacggcaat 1620
ctaatatttg gcaaagaagg gacaacggca agtaacgcag aattagataa tgtaatgatt 1680
acggatgaag aagagattcg taccaccaat cctgtggcaa cagagcagta tggaactgtg 1740
gcaaataact tgcagagctc aaatacagct cccacgactg gaactgtcaa tcatcagggg 1800
gccttacctg gcatggtgtg gcaagatcgt gacgtgtacc ttcaaggacc tatctgggca 1860
aagattcctc acacggatgg acactttcat ccttctcctc tgatgggagg ctttggactg 1920
aaacatccgc ctcctcaaat catgatcaaa aatactccgg taccggcaaa tcctccgacg 1980
actttcagcc cggccaagtt tgcttcattt atcactcagt actccactgg acaggtcagc 2040
gtggaaattg agtgggagct acagaaagaa aacagcaaac gttggaatcc agagattcag 2100
tacacttcca actacaacaa gtctgttaat gtggacttta ctgtagacac taatggtgtt 2160
tatagtgaac ctcgccctat tggaacccgg tatctcacac gaaacttgta a 2211
<210>201
<211>2205
<212>DNA
<213>腺伴随病毒,血清型4-4
<400>201
atgactgacg gttaccttcc agattggcta gaggacaacc tctctgaagg cgttcgagag 60
tggtgggcgc tgcaacctgg agcccctaaa cccaaggcaa atcaacaaca tcaggacaac 120
gctcggggtc ttgtgcttcc gggttacaaa tacctcggac ccggcaacgg actcgacaag 180
ggggaacccg tcaacgcagc ggacgcggca gccctcgagc acgacaaggc ctacgaccag 240
cagctcaagg ccggtgacaa cccctacctc aagtacaacc acgccgacgc ggagttccag 300
cagcggcttc agggcgacac atcgtttggg ggcaacctcg gcagagcagt cttccaggcc 360
aaaaagaggg ttcttgaacc tcttggtctg gttgagcaag cgggtgagac ggctcctgga 420
aagaagagac cgttgattga atccccccag cagcccgact cctccacggg tatcggcaaa 480
aaaggcaagc agccggctaa aaagaagctc gttttcgaag acgaaactgg agcaggcgac 540
ggaccccctg agggatcaac ttccggagcc atgtctgatg acagtgagat gcgtgcagca 600
gctggcggag ctgcagtcga gggcggacaa ggtgccgatg gagtgggtaa tgcctcgggt 660
gattggcatt gcgattccac ctggtctgag ggccacgtca cgaccaccag caccagaacc 720
tgggtcttgc ccacctacaa caaccacctc tacaagcgac tcggagagag cctgcagtcc 780
aacacctaca acggattctc caccccctgg ggatactttg acttcaaccg cttccactgc 840
cacttctcac cacgtgactg gcagcgactc atcaacaaca actggggcat gcgacccaaa 900
gccatgcggg tcaaaatctt caacatccag gtcaaggagg tcacgacgtc gaacggcgag 960
acaacggtgg ctaataacct taccagcacg gttcagatct ttgcggactc gtcgtacgaa 1020
ctgccgtacg tgatggatgc gggtcaagag ggcagcctgc ctccttttcc caacgacgtc 1080
tttatggtgc cccagtacgg ctactgtgga ctggtgaccg gcaacacttc gcagcaacag 1140
actgacagaa atgccttcta ctgcctggag tactttcctt cgcagatgct gcggactggc 1200
aacaactttg aaattacgta cagttttgag aaggtgcctt tccactcgat gtacgcgcac 1260
agccagagcc tggaccggct gatgaaccct ctcatcgacc agtacctgtg gggactgcaa 1320
tcgaccacca ccggaaccac cctgaatgcc gggactgcca ccaccaactt taccaagctg 1380
cggcctacca acttttccaa ctttaaaaag aactggctgc ccgggccttc aatcaagcag 1440
cagggcttct caaagactgc caatcaaaac tacaagatcc ctgccaccgg gtcagacagt 1500
ctcatcaaat acgagacgca cagcactctg gacggaagat ggagtgccct gacccccgga 1560
cctccaatgg ccacggctgg acctgcggac agcaagttca gcaacagcca gctcatcttt 1620
gcggggccta aacagaacgg caacacggcc accgtacccg ggactctgat cttcacctct 1680
gaggaggagc tggcagccac caacgccacc gatacggaca tgtggggcaa cctacctggc 1740
ggtgaccaga gcaacagcaa cctgccgacc gtggacagac tgacagcctt gggagccgtg 1800
cctggaatgg tctggcaaaa cagagacatt tactaccagg gtcccatttg ggccaagatt 1860
cctcataccg atggacactt tcacccctca ccgctgattg gtgggtttgg gctgaaacac 1920
ccgcctcctc aaatttttat caagaacacc ccggtacctg cgaatcctgc aacgaccttc 1980
agctctactc cggtaaactc cttcattact cagtacagca ctggccaggt gtcggtgcag 2040
attgactggg agatccagaa ggagcggtcc aaacgctgga accccgaggt ccagtttacc 2100
tccaactacg gacagcaaaa ctctctgttg tgggctcccg atgcggctgg gaaatacact 2160
gagcctaggg ctatcggtac ccgctacctc acccaccacc tgtaa 2205
<210>202
<211>2211
<212>DNA
<213>腺伴随病毒,血清型1
<400>202
atggctgccg atggttatct tccagattgg ctcgaggaca acctctctga gggcattcgc 60
gagtggtggg acttgaaacc tggagccccg aagcccaaag ccaaccagca aaagcaggac 120
gacggccggg gtctggtgct tcctggctac aagtacctcg gacccttcaa cggactcgac 180
aagggggagc ccgtcaacgc ggcggacgca gcggccctcg agcacgacaa ggcctacgac 240
cagcagctca aagcgggtga caatccgtac ctgcggtata accacgccga cgccgagttt 300
caggagcgtc tgcaagaaga tacgtctttt gggggcaacc tcgggcgagc agtcttccag 360
gccaagaagc gggttctcga acctctcggt ctggttgagg aaggcgctaa gacggctcct 420
ggaaagaaac gtccggtaga gcagtcgcca caagagccag actcctcctc gggcatcggc 480
aagacaggcc agcagcccgc taaaaagaga ctcaattttg gtcagactgg cgactcagag 540
tcagtccccg atccacaacc tctcggagaa cctccagcaa cccccgctgc tgtgggacct 600
actacaatgg cttcaggcgg tggcgcacca atggcagaca ataacgaagg cgccgacgga 660
gtgggtaatg cctcaggaaa ttggcattgc gattccacat ggctgggcga cagagtcatc 720
accaccagca cccgcacctg ggccttgccc acctacaata accacctcta caagcaaatc 780
tccagtgctt caacgggggc cagcaacgac aaccactact tcggctacag caccccctgg 840
gggtattttg atttcaacag attccactgc cacttttcac cacgtgactg gcagcgactc 900
atcaacaaca attggggatt ccggcccaag agactcaact tcaaactctt caacatccaa 960
gtcaaggagg tcacgacgaa tgatggcgtc acaaccatcg ctaataacct taccagcacg 1020
gttcaagtct tctcggactc ggagtaccag cttccgtacg tcctcggctc tgcgcaccag 1080
ggctgcctcc ctccgttccc ggcggacgtg ttcatgattc cgcaatacgg ctacctgacg 1140
ctcaacaatg gcagccaagc cgtgggacgt tcatcctttt actgcctgga atatttccct 1200
tctcagatgc tgagaacggg caacaacttt accttcagct acacctttga ggaagtgcct 1260
ttccacagca gctacgcgca cagccagagc ctggaccggc tgatgaatcc tctcatcgac 1320
caatacctgt attacctgaa cagaactcaa aatcagtccg gaagtgccca aaacaaggac 1380
ttgctgttta gccgtgggtc tccagctggc atgtctgttc agcccaaaaa ctggctacct 1440
ggaccctgtt atcggcagca gcgcgtttct aaaacaaaaa cagacaacaa caacagcaat 1500
tttacctgga ctggtgcttc aaaatataac ctcaatgggc gtgaatccat catcaaccct 1560
ggcactgcta tggcctcaca caaagacgac gaagacaagt tctttcccat gagcggtgtc 1620
atgatttttg gaaaagagag cgccggagct tcaaacactg cattggacaa tgtcatgatt 1680
acagacgaag aggaaattaa agccactaac cctgtggcca ccgaaagatt tgggaccgtg 1740
gcagtcaatt tccagagcag cagcacagac cctgcgaccg gagatgtgca tgctatggga 1800
gcattacctg gcatggtgtg gcaagataga gacgtgtacc tgcagggtcc catttgggcc 1860
aaaattcctc acacagatgg acactttcac ccgtctcctc ttatgggcgg ctttggactc 1920
aagaacccgc ctcctcagat cctcatcaaa aacacgcctg ttcctgcgaa tcctccggcg 1980
gagttttcag ctacaaagtt tgcttcattc atcacccaat actccacagg acaagtgagt 2040
gtggaaattg aatgggagct gcagaaagaa aacagcaagc gctggaatcc cgaagtgcag 2100
tacacatcca attatgcaaa atctgccaac gttgatttta ctgtggacaa caatggactt 2160
tatactgagc ctcgccccat tggcacccgt taccttaccc gtcccctgta a 2211
<210>203
<211>2211
<212>DNA
<213>腺伴随病毒,血清型6
<400>203
atggctgccg atggttatct tccagattgg ctcgaggaca acctctctga gggcattcgc 60
gagtggtggg acttgaaacc tggagccccg aaacccaaag ccaaccagca aaagcaggac 120
gacggccggg gtctggtgct tcctggctac aagtacctcg gacccttcaa cggactcgac 180
aagggggagc ccgtcaacgc ggcggatgca gcggccctcg agcacgacaa ggcctacgac 240
cagcagctca aagcgggtga caatccgtac ctgcggtata accacgccga cgccgagttt 300
caggagcgtc tgcaagaaga tacgtctttt gggggcaacc tcgggcgagc agtcttccag 360
gccaagaaga gggttctcga accttttggt ctggttgagg aaggtgctaa gacggctcct 420
ggaaagaaac gtccggtaga gcagtcgcca caagagccag actcctcctc gggcattggc 480
aagacaggcc agcagcccgc taaaaagaga ctcaattttg gtcagactgg cgactcagag 540
tcagtccccg acccacaacc tctcggagaa cctccagcaa cccccgctgc tgtgggacct 600
actacaatgg cttcaggcgg tggcgcacca atggcagaca ataacgaagg cgccgacgga 660
gtgggtaatg cctcaggaaa ttggcattgc gattccacat ggctgggcga cagagtcatc 720
accaccagca cccgaacatg ggccttgccc acctataaca accacctcta caagcaaatc 780
tccagtgctt caacgggggc cagcaacgac aaccactact tcggctacag caccccctgg 840
gggtattttg atttcaacag attccactgc catttctcac cacgtgactg gcagcgactc 900
atcaacaaca attggggatt ccggcccaag agactcaact tcaagctctt caacatccaa 960
gtcaaggagg tcacgacgaa tgatggcgtc acgaccatcg ctaataacct taccagcacg 1020
gttcaagtct tctcggactc ggagtaccag ttgccgtacg tcctcggctc tgcgcaccag 1080
ggctgcctcc ctccgttccc ggcggacgtg ttcatgattc cgcagtacgg ctacctaacg 1140
ctcaacaatg gcagccaggc agtgggacgg tcatcctttt actgcctgga atatttccca 1200
tcgcagatgc tgagaacggg caataacttt accttcagct acaccttcga ggacgtgcct 1260
ttccacagca gctacgcgca cagccagagc ctggaccggc tgatgaatcc tctcatcgac 1320
cagtacctgt attacctgaa cagaactcag aatcagtccg gaagtgccca aaacaaggac 1380
ttgctgttta gccgggggtc tccagctggc atgtctgttc agcccaaaaa ctggctacct 1440
ggaccctgtt accggcagca gcgcgtttct aaaacaaaaa cagacaacaa caacagcaac 1500
tttacctgga ctggtgcttc aaaatataac cttaatgggc gtgaatctat aatcaaccct 1560
ggcactgcta tggcctcaca caaagacgac aaagacaagt tctttcccat gagcggtgtc 1620
atgatttttg gaaaggagag cgccggagct tcaaacactg cattggacaa tgtcatgatc 1680
acagacgaag aggaaatcaa agccactaac cccgtggcca ccgaaagatt tgggactgtg 1740
gcagtcaatc tccagagcag cagcacagac cctgcgaccg gagatgtgca tgttatggga 1800
gccttacctg gaatggtgtg gcaagacaga gacgtatacc tgcagggtcc tatttgggcc 1860
aaaattcctc acacggatgg acactttcac ccgtctcctc tcatgggcgg ctttggactt 1920
aagcacccgc ctcctcagat cctcatcaaa aacacgcctg ttcctgcgaa tcctccggca 1980
gagttttcgg ctacaaagtt tgcttcattc atcacccagt attccacagg acaagtgagc 2040
gtggagattg aatgggagct gcagaaagaa aacagcaaac gctggaatcc cgaagtgcag 2100
tatacatcta actatgcaaa atctgccaac gttgatttca ctgtggacaa caatggactt 2160
tatactgagc ctcgccccat tggcacccgt tacctcaccc gtcccctgta a 2211
<210>204
<211>2208
<212>DNA
<213>新AAV血清型,克隆hu.63
<400>204
atggctgccg atggttatct tccagattgg ctcgaggaca ctctctctga aggaataaga 60
cagtggtgga agctcaaacc tggcccacca ccaccaaagc ccgcagagcg gcataaggac 120
gacagcaggg gtcttgtgct tcctgggtac aagtaccttg gacccttcaa cggactcgac 180
aagggagagc cggtcaacga ggcagacgcc gcggccctcg agcacgacaa ggcctacgac 240
cggcagctcg acagcggaga caacccgtac cccaagtaca accacgccga cgcggagttc 300
caggagcgtc ttaaagaaga tacgtctttt gggggcaacc tcggacgagc agtcttccag 360
gcgaaaaaga gggttcttga acctctgggc ctggttgagg aacctgttaa gacggctccg 420
ggaaaaaaga ggccggtaga gcactctcct gcggagccag actcctcctc gggaaccgga 480
aaagcgggcc agcagcctgc aagaaagaga ttgaatttcg gtcagactgg agacgcagac 540
tccgtacctg acccccagcc tctcggacag ccaccagcag ccccctctgg tctgggaact 600
aatacgatgg ctacaggcag tggcgcacca atggcagaca ataacgaggg cgccgacgga 660
gtgggtaatt cctcgggaaa ttggcattgc gattccacat ggatgggcga cagagtcatc 720
accaccagca cccgcacctg ggctctgccc acctacaaca accacctcta caagcagatt 780
tccagccaat caggagcctc aaacgacaac cactactttg gctacagcac cccttggggg 840
tattttgact tcaacagatt ccactgccac ttttcgccac gtgactggca aagactcatc 900
aacaacaatt ggggattccg gcccaaaaga ctcaacttca agctctttaa cattcaagtc 960
aaggaggtca cgcagaatga cggtacgacg acgattgcca ataaccttac cagcacggtt 1020
caggtgttta ctgactcgga gtaccagctc ccgtacgtcc tcggctcggc gcatcaagga 1080
tgcctcccgc cgttcccagc agacgtcttc atggtgccac agtatggata cctcaccctg 1140
aacaacggga gtcaggcagt aggacgctct tcattttact gcctggagta ctttccttct 1200
cagatgctgc gtaccggaaa caactttacc ttcagctaca cctttgagga cgttcctttc 1260
cacagcagct acgcccacag ccagagtttg gaccgtctca tgaatcctct catcgaccag 1320
tacctgtatt acttgagcag aacaaacact ccaagcggaa ccaccacgca gtcaaggctt 1380
cagttttctc aggccggagc aagtgacatt cgggaccagt ctaggaactg gcttcctgga 1440
ccctgttacc gccagcagcg agtatcaaag acatctgcgg ataacaacaa cagtgaatac 1500
tcgtggactg gagctaccaa gtaccacctt aatggaagag actctctggt gaatccgggc 1560
ccggccatgg ccagccacaa ggacgatgaa gaaaagtttt ttcctcagag cggggttctc 1620
atctttggaa aacaagactc gggaaaaact aatgtggaca ttgaaaaggt catgattaca 1680
gacgaagagg aaatcaggac caccaatccc gtggctacgg agcagtatgg ttctgtatct 1740
accaacctcc agagcggcaa cacacaagca gctacctcag atgtcaacac acaaggcgtt 1800
cttccaggca tggtgtggca ggacagagac gtgtacctgc aggggcccat ctgggcaaag 1860
attccacaca cggacggaca ttttcacccc tctcccctca tgggcggatt cggacttaaa 1920
caccctcccc cgcagattct catcaagaac accccggtac ctgcgaatcc ttcgactacc 1980
ttcagtgcgg caaagtttgc ttccttcatt acacagtact ccacggggca ggtcagcgtg 2040
gagatcgagt gggagctgca gaaggagaac agcaaacgct ggaatcccga gattcagtac 2100
acttccaact acaacaaatc tgttaatgtg gactttactg tggacactaa tggtgtgtat 2160
tcagagcctc gccccattgg caccaggtac ctgactcgta atctgtaa 2208
<210>205
<211>2208
<212>DNA
<213>新AAV血清型,克隆hu.56
<400>205
atggctgccg atggttatct tccagattgg ctcgaggaca ctctctctga aggaataaga 60
cagtggtgga agctcaaacc tggcccacca ccaccaaagc ccgcagagcg gcataaggac 120
gacagcaggg gtcttgtgct tcctggatac aagtacctcg gacccttcaa cggactcgac 180
aagggagagc cggtcaacga ggcagacgcc gcggccctcg agcacgacaa ggcctacgac 240
cggcagctcg acagcggaga caacccgtac ctcaagtaca accacgccga cgcggagttt 300
caggagcgcc ttaaagaaga tacgtctttt gggggcaacc tcggacgagc agtcttccag 360
gcgaaaaaga gggttcttga acctctgggc ctggttgagg aacctgttaa gacggctccg 420
ggaaaaaaga ggccggtaga gcactctcct gtggagccag actcctcctc gggaaccgga 480
aaagcgggca accagcctgc aagaaaaaga ttgaatttcg gtcagactgg agacgcagac 540
tccgtacctg acccccagcc tctcggacag ccaccagcat ccccctctgg tctgggaact 600
aatacgatgg ctacaggcag tggcgcacca atggcagaca ataacgaggg cgccgacgga 660
gtgggtaatt cctcgggaaa ttggcattgc gattccacat ggatgggcga cagagtcgtc 720
accaccagca cccgcacctg ggccctgccc acctacaaca accacctcta caagcagatt 780
tccagccaat caggagccag caacgacaac cactactttg gctacagcac cccttggggg 840
tattttgact tcaacagatt ccactgccac ttttcgccac gcgactggca gagactcatc 900
aacaacaact ggggattccg gcccaaaaga ctcaacttca agctgtttaa cattcaagtc 960
aaggaggtca cgcagaatga cggtacgacg acgattgcca ataaccttac cagcacggtt 1020
caggtgttta ctgacttgga gtaccagctc ccgtacgtcc tcggctcggc gcatcaagga 1080
tgcctcccgc cgttcccagc agacgtcttc atggtgccac agtatggata cctcaccctg 1140
aacaacggga gtcaggcggt aggacgctct tccttttact gcctggagta ctttccttct 1200
cagatgcttc gcaccggaaa caactttacc ttcagctaca cttttgaaga cgttcctttc 1260
cacagcagct acgctcacag tcaaagtctg gaccgtctca tgaatcctct catcgaccag 1320
tacctgtatt acttgagcag aacaaacact ccaagcggaa ccactacgca gtccaggctt 1380
cagttttctc aggccggagc gagtgacatt cgggaccagt ctaggaactg gcttcctgga 1440
ccctgttacc gccagcagcg agtatcaaag acagctgcgg ataacaacaa cagtgaatac 1500
tcgtggactg gagctaccaa gtaccacctc aatggcagag actctctggt gaatccgggc 1560
ccggccatgg ccagccacaa ggacgatgaa gaaaagtttt ttcctcaaag cggggttctc 1620
atctttggga agcaaggctc agagaaaaca aatgtggaca ttgaaaaggt catgattaca 1680
gacgaagagg aaatcaggac caccaatccc gtggctacgg agcagtatgg ttctgtatct 1740
accaacctcc agagcggcaa cacacaagca gctacctcag atgtcaacac acaaggcgtt 1800
cttccaggca tggtctggca ggacagagac gtgtacctgc aggggcccat ctgggcaaaa 1860
attccacaca cggacggaca ttttcacccc tctcccctca tgggcggatt tggacttaaa 1920
caccctcctc cacagattct cattaagaat accccggtac ctgcgaatcc ttcgaccacc 1980
ttcagcgcgg caaagtttgc ttccttcatc acacagtatt ccacggggca ggtcagcgtg 2040
gagatcgagt gggagctgca gaaggagaac agcaaacgct ggaatcccga aattcagtac 2100
acttccaact acaacaaatc tgttaatgtg gactttactg tggacactaa tggggtgtat 2160
tcagagcctc gccctattgg caccagatac ctgactcgta atctgtaa 2208
<210>206
<211>2205
<212>DNA
<213>新AAV血清型,克隆hu.57
<400>206
atggctgccg atggttatct tccagattgg ctcgaggaca ctctctctga aggaataaga 60
cagtggtgga agctcaaacc tggcccacca ccaaagcccg cagagcggca taaggacgac 120
agcaggggtc ttgtgcttcc tggatacaag tacctcggac ccttcaacgg actcgacaag 180
ggagagccgg tcaacgaggc agacgccgcg gccctcgagc acgacaaggc ctacgaccgg 240
cagctcgaca gcggagacaa cccgtacctc aagtacaacc acgccgacgc ggagtttcag 300
gagcgcctta aagaagatac gtcttttggg ggcaacctcg gacgagcagt cttccaggcg 360
aaaaagaggg ttcttgaacc tctgggcctg gttgaggaac ctgttaagac ggctccggga 420
aaaaagaggc ccgtagagca ctctcctgtg gagccagact cctcctcggg aaccggaaaa 480
gcgggcaacc agcctgcaag aaaaagattg aatttcggtc agactggaga cgcagactcc 540
gtacctgacc cccagcctct cggacagcca ccagcagccc cctctggtct gggaactaat 600
acgatggcta caggcagtgg cgcaccaatg gcagacaata acgagggcgc cgacggagtg 660
ggtaattcct cgggagattg gcattgcgat tccacatgga tgggcgacag agtcatcacc 720
accagcaccc gcacctgggc cctgcccacc tacaacaacc acctctacaa gcagatttcc 780
agccaatcag gagccagcaa tgacaaccac tactttggct acagcacccc ttgggggtat 840
tttgacttca acagattcca ctgccacttt tcgccacgcg actggcagag actcatcaac 900
aacaactggg gattccggcc caaaagactc aacctcaagc tgtttaacat tcaagtcaag 960
gaggtcacgc agaatgacgg tacgacgacg attgccaata accttaccag cacggttcag 1020
gtgtttactg acttggagta ccagctcccg tacgtcctcg gctcggcgca tcaaggatgc 1080
ctcccgccgt tcccagcaga cgtcttcatg gtgccacagt atggatacct caccctgaac 1140
aacgggagtc aggcggtagg acgctcttcc ttttactgcc tggagtactt tccttctcag 1200
atgcttcgta ccggaaacaa ctttaccttc agctacactt ttgaagacgt tcctttccac 1260
agcagctacg ctcacagtca aagtctggac cgtctcatga atcctctcat cgaccagtac 1320
ctgtattact tgagcagaac aaacactcca agcggaacca ctacgcagtc caggcttcag 1380
ttttctcagg ccggagcgag tgacattcgg gaccagtcta ggaactggct tcctggaccc 1440
tgttaccgcc agcagcgagt atcaaagaca gctgcggata acaacaacgg tgaatactcg 1500
tggactggag ctaccaagta ccacctcaat ggcagagact ctctggtgaa tccgggcccg 1560
gccatggcca gccacaagga cgatgaagaa aagttttttc ctcaaagcgg ggttctcatc 1620
tttgggaagc aaggctcaga gaaaacaaat gtggacattg aaaaggtcat gattacagac 1680
gaagaggaaa tcaggaccac caatcccgtg gctacggagc agtatggttc tgtatctacc 1740
aacctccaga gcggcaacac acgagcagct acctcagatg tcaacacaca aggcgttctt 1800
ccaggcatgg tctggcagga cagagacgtg tacctgcagg ggcccatctg ggcaaaaatt 1860
ccacacacgg acggacattt tcacccctct cccctcatgg gcggatttgg acttaaacac 1920
cctcctccac agattctcat taagaatacc ccggtacctg cgaatccttc gaccaccttc 1980
agcgcggcaa agtttgcttc cttcatcaca cagtattcca cggggcaggt cagcgtggag 2040
atcgagtggg agctgcagaa ggagaacagc aaacgctgga atcccgaaat tcagtacact 2100
tccaactaca acaaatctgt taatgtggac tttactgtgg acactaatgg ggtgtattca 2160
gagcctcgcc ctattggcac cagatacctg actcgtaatc tgtaa 2205
<210>207
<211>2208
<212>DNA
<213>新AAV血清型,克隆hu.58
<400>207
atggctgccg atggttatct tccagattgg ctcgaggaca ctctctctga aggaataaga 60
cagtggtgga agctcaaacc tggcccacca ccaccaaagc ccgcagagcg gcataaggac 120
gacagcaggg gtcttgtgct tcctgggtac aagtacctcg gacccttcaa cggactcgac 180
aagggagagc cggtcaacga ggcagacgcc gcggccctcg agcacgacaa ggcctacgac 240
cggcagctcg acagcggaga caacccgtac ctcaagtacg accacgccga cgcggagttt 300
caggagcgcc ttaaagaaga tacgtctttt gggggcaacc tcggacgagc agtcttccag 360
gcaaaaaaga gggttcttga acctctgggc ctggttgagg agcctgttaa gacggctccg 420
ggaaaaaaga ggccggtaga gcactctcct gtggagccag actcctcctc gggaaccgga 480
aaagcgggca accagcctgc aagaaagaga ttgaatttcg gtcagactgg agacgcagac 540
tccgtacctg acccccagcc tctcggacag ccaccagcag ccccctctgg tctgggaact 600
aatacgatgg ctacaggcag tggcgcacca atggcagaca ataacgatgg cgccgacgga 660
gtgggtaatt cctcgggaaa ttggcattgc gattccacat ggatgggcga cagagtcatc 720
accaccagca cccgaacctg ggctctgccc acctacaaca accatctgta caagcagatt 780
tccagccaat caggagccag caacgacaac cactactttg gctacagcac cccttggggg 840
tattttgact tcaacagatt ccactgccac ttttcaccac gtgactggca aagactcatc 900
aacaacaact ggggattccg gcccaagaga ctcaacttca agctctttaa cattcaagtc 960
agagaggtca cgcagaatga tggtacgacg acgattgcca ataaccttac cagcacggtt 1020
caggtgttta ctgactcgga gtaccagctc ccgtacgtcc tcggctcggc gcatcaagga 1080
tgcctcccgc cgttcccagc agacgtcttc atggtgccac agtatggata cctcaccctg 1140
aacaacggga gtcaggcagt aggacgctct tcattttact gcctggagta cttcccttct 1200
cagatgctgc gtaccggaaa caactttacc ttcagctaca cctttgagga tgttcctttc 1260
cacagcagct acgctcacag ccagagtttg gaccgtctca tgaatcctct catcgaccag 1320
tacctgtatt acttgagcag aacaaacact ccaagcggaa ccaccacgca gtccaggctt 1380
cagttttctc aggccggagc gagtgacatt cgggatcagt ctaggaactg gcttcctgga 1440
ccctgttacc gccagcagcg agtatcaaag acatctgcgg acaacaacaa cagtgaatac 1500
tcgtggattg gagctaccaa gtaccacctc aatggcagag actctctggt gaatccgggc 1560
ccggcaatgg ccagccacaa ggacgatgaa gaaaagtttt ttcctcagag cggggttctc 1620
atctttggaa aacaaggatc agagaaaaca aatgtggaca ttgaaaaggt catgattaca 1680
gacgaagagg aaatcagaac caccaatccc gtggccacgg agcagtatgg ttctgtatct 1740
accaacctcc agagcggcaa cacacaagca tctactgcag atgtcaacac acaaggcgtt 1800
cttccaggca tggtctggca ggacagagac gtgtacctgc aggggcctat ctgggcaaag 1860
attccgcaca cggacggaca ttttcacccc tctcccctca tgggcggatt cggacttaaa 1920
caccctcctc cacagattct catcaaaaac accccggtac ctgcgaatcc ttcgaccacc 1980
ttcagtgcgg caaagtttgc ttccttcatc acacagtatt ccacggggcg ggtcagcgtg 2040
gagatcgagt gggagctaca gaaggagaac agcaaacgct ggaatcccga gatccagtac 2100
acttccaact acaacaaatc tgttaatgtg gactttactg tggacactaa tggtgtgtat 2160
tcagagcctc gccccattgg caccagatac ctgactcgta atctgtaa 2208
<210>208
<211>2208
<212>DNA
<213>新AAV血清型,克隆hu.51
<400>208
atggctgccg atggttatct tccagattgg ctcgaggaca ctctctctga aggaataaga 60
cagtggtgga agctcaaacc tggcccacca ccaccaaagc ccgcagagcg gcataaggac 120
gacagcaggg gtcttgtgct tcctgggtac aagtacctcg gacccttcaa cggactcgac 180
aagggagagc cggtcaacga ggcagacgcc gcggccctcg agcacgacaa agcctacgac 240
cggcagctcg acagcggaga caacccgtac ctcaagtaca accacgccga cgcggagttt 300
caggagcgcc ttaaagaaga tacgtctttt gggggcaacc tcggacgagc agtcttccag 360
gcgaaaaaga gggttcttga acctctgggc ctggttgggg aacctgtcaa gacggctcca 420
ggaaaaaaga ggccggtaga gcactctcct gtggagccag actcctcctc gggaaccgga 480
aaggcgggcc agcagcctgc aagaaaaaga ttgaattttg gtcagactgg agacgcagac 540
tcagtacctg acccccagcc tctcggacag ccaccagcag ccccctctgg tctgggaact 600
aatacgatgg ctacaggcag tggcgcacca atggcagaca ataacgaggg cgccgacgga 660
gtgggtaatt cctcgggaaa ttggcattgc gattccacat ggatgggcga cagagtcatc 720
accaccagca cccgaacctg ggccctgccc acctacaaca accacctcta caaacaaatt 780
tccagccaat caggagcctc gaacgacaat cactactttg gctacagcac cccttggggg 840
tattttgact tcaacagatt ccactgccac ttttcaccac gtgactggca aagactcatc 900
aacaacaact ggggattccg acccaagaga ctcaacttca agctctttaa cattcaagtc 960
aaagaggtca cgcagaatga cggtacgacg acgattgcca ataaccttac cagcacggtt 1020
caggtgttta ctgactcgga gtaccagctc ccgtacgtcc tcggctcggc gcatcaagga 1080
tgcctcccgc cgttcccagc agacgtcttc atggtgccac agtatggata cctcaccctg 1140
aacaacggga gtcaggcagt aggacgctct tcattttact gcctggagta ctttccttct 1200
cagatgctgc gtaccggaaa caactttacc ttcagctaca cttttgagga cgttcctttc 1260
cacagcggct acgctcacag ccagagtctg gaccgtctca tgaatcctct catcgaccag 1320
tacctgtatt acttgagcac aacaaacact ccaagtggaa ccaccacgca gtcaaggctt 1380
cagttttctc aggccggagc gagtgacatt cgggaccagt ctaggaactg gcttcctgga 1440
ccctgttacc gccagcagcg agtatcaaag acatctgcgg ataacaacaa cagtgaatac 1500
tcgtggactg gagctaccaa gtaccacctc aatggcagag actctctggt gaatccgggc 1560
ccggccatgg caagccacaa ggacaatgaa gaaaagtttt ttcctcagag cggggttctc 1620
atctttggga agcaaggctc agagaaaaca aatgtggaca ttgaaaaggt catgattaca 1680
gacgaagagg aaatcaggac aaccaatccc gtggctacgg agcagtacgg ttctgtatct 1740
accaacctcc agagaggcaa cagacaagca gctaccgcag atgtcaacac acaaggcgtt 1800
cttccaggca tggtctggca ggacagagat gtgtaccttc aggggcccat ctgggcaaag 1860
attccacaca cggacggaca ttttcacccc tctcccctca tgggtggatt cggacttaaa 1920
caccctcctc cacagattct catcaagaac accccggtac ctgcgaatcc ttcgaccacc 1980
ttcagtgcgg caaagtttgc ttccttcatc acacagtact ccacgggaca ggtcagcgtg 2040
gagatcgagt gggagctgca gaaggaaaac agcaaacgct ggaatcccga aattcagtac 2100
acttccaact acaacaagtc tgttaatgtg gactttactg tggacactaa tggcgtgtat 2160
tcagagcctc gccccattgg caccagatac ctgactcgta atctgtaa 2208
<210>209
<211>2208
<212>DNA
<213>新AAV血清型,克隆hu.49
<400>209
atggctgccg atggttatct tccagattgg ctcaaggaca ctctctctga aggaataaga 60
cagtggtgga agctcaaacc tggcccacca ccaccaaagc ccgcagagcg gcataaggac 120
gacagcgggg gtcttgtgct tcctgggtac aagtacctcg gacccttcaa cggactcgac 180
aagggagagc cggtcaacga ggcagacgcc gcggccctcg agtacgacaa ggcctacgac 240
cggcagctcg acagcggaga caacccgtac ctcaagtaca accacgccga cgcggagttt 300
caggagcgcc ttaaagaaga tacgtctttt gggggcaacc tcggacgagc agtcttccag 360
gcgaaaaaga gggttcttga acctctgggc ctggttgagg aacctgttaa gacggctccg 420
ggaaaaaaga ggccggtaga gcactctcct gtggagccag actcctcctc gggaacagga 480
aaagcgggcc agcagcctgc gagaaagaga ttgaattttg gtcagactgg agacgcagac 540
tccgtacctg acccccagcc tctcggacag ccaccagcag ccccctctgg tctgggaact 600
aatacgatgg ctacaggcag tggcgcacca atggcagaca ataacgaggg cgccgacgga 660
gtgggtaatt cctcgggaag ttggcattgc gattccacat ggatgggcga cagagtcatc 720
accaccagca cccgaacctg ggctctgccc acctacaaca accatctgta caagcagatc 780
tccagccaat caggagccag caacgacaac cactactttg gctacagcac cccttggggg 840
tattttgact tcaacagatt ccactgccac ttttcaccac gtgactggca aagactcatc 900
aacaacaact ggggattccg gcccaagaga ctcaacttca agctctttaa cattcaagtc 960
aaggaggtca cgcagaatga cggtacgacg acgattgcca ataaccttac cagcacggtt 1020
caggtgttta ctgactcgga gtaccagctc ccgtacgtcc ccggctcggc gcatcaagga 1080
tgcctcccgc cgttcccagc agacgtcttc atggtgccac agtatggata cctcaccctg 1140
aacaacggga gtcaggcagt aggacgctct tcattttact gcctggagta ctttccttct 1200
cagatgcttc gtaccggaaa caactttacc ttcagctaca cctttgagga tgttcctttc 1260
cacagcagct acgctcacag ccagagtttg gaccgtctca tgaatcctct catcgaccag 1320
tacctgtatt acttgagcag aacaaacact ccaagcggaa ccaccacgca gtccaggctt 1380
cagttttctc aggccggagc aagtgacatt cgggaccagt ctaggaactg gcttcctgga 1440
ccctgttacc gccagcagcg agtatcaaag acagctgcgg acaacaacaa cagtgaatac 1500
tcgtggactg gagctaccaa gtaccacctc aatggcagag actctctggt gaatccgggc 1560
ccggccatgg ccagccacaa ggacgatgaa gaaaagtttt ttcctcaaag cggggttctc 1620
atctttggga agcaaggctc agagaaaaca aatgtggaca ttgaaaaggt catgattaca 1680
gacgaagagg aaatcagaac caccaatccc gtggccacgg agcagtatgg ttctgtatct 1740
accaacctcc agagcggcaa cacacaagca gctactgcag atgtcaacac acaaggcgtt 1800
cttccaggca tggtctggca ggacagagac gtgcacctgc aggggcctat ctgggcaaag 1860
attccacaca cggacggaca ttttcacccc tctcccctca tgggcggatt tggacttaaa 1920
caccctcctc cacagattct catcaagaac accccggtac ctgcaaatcc ttcgaccacc 1980
ttcagtgcgg caaagtttgc ttccttcatc acacagtatt ccacagggca ggtcagcgtg 2040
gagatcgagt gggagctgca gaaggagaac agcaaacgct ggaaccccga gatccagtac 2100
acttccaact acaacaaatc tgttaatgtg gactttactg tggacactaa tggtgtgtat 2160
tcagagcctc gccccattgg caccagatac ctgactcgta atctgtaa 2208
<210>210
<211>2208
<212>DNA
<213>新AAV血清型,克隆hu.52
<400>210
atggctgccg atggttatct tccagattgg ctcgaggaca ctctctctga aggaataaga 60
cagtggtgga agctcaaacc tggcccacca ccaccaaagc ccgcagagcg gcataaggac 120
gacagcaggg gtcttgtgct tcctgggtac aagtacctcg gacccttcaa cggactcgac 180
aagggagagc cggtcaacga ggcagacgcc gcggccctcg agcacgacaa agcctacgac 240
cggcagctcg acagcggaga caacccgtac ctcaagtaca accacgccga cgcggagttt 300
caggagcgcc ttaaagaaga tacgtctttt gggggcaacc tcggacgagc agtcttccag 360
gcgaaaaaga gggttcttga acctctgggc ctggttgggg aacctgttaa gacggctccg 420
ggaaaaaaga ggccggtaga gcactctcct gtggagccag actcctcctc gggaaccgga 480
aaggcgggcc agcagcctgc aagaaaaaga ttgaattttg gtcagactgg agacgcagac 540
tcagtacctg acccccagcc tctcggacag ccaccagcag ccccctctgg tctgggaact 600
aatacgatgg ctacaggcag tggcgcacca atggcagaca ataacgaggg cgccgacgga 660
gtgggtaatt cctcgggaaa tcggcattgc gattccacat ggatgggcga cagagtcatc 720
accaccagca cccgaacctg ggccctgccc acctacaaca accacctcta cagacaaatt 780
tccagccaat caggagcctc gaacgacaat cactactttg gctacagcac cccttggggg 840
tattttgact tcaacagatt ccactgccac ttttcaccac gtgactggca aagactcatc 900
aacaacaact ggggattccg acccaagaga ctcaacttca agctctttaa cattcaagtc 960
aaagaggtca cgcagaatga cggtacgacg acgattgcca ataaccttac cagcacggtt 1020
caggtgttta ctgactcgga gtaccagctc ccgtacgtcc tcggctcggc gcatcaagga 1080
tgcctcccgc cgttcccagc agacgtcttc atggtgccac agtatggata cctcaccctg 1140
agcaacggga gtcaggcagt aggacgctct tcattttact gcccggagta ctttccttct 1200
cagatgctgc gtaccggaaa caactttacc ttcagctaca cttttgagga cgttcctttc 1260
cacagcagct acgctcacag ccagagtctg gaccgtctca tgaatcctct catcgaccag 1320
tacctgtatt acttgagcac aacaaacact ccaagtggaa ccaccacgca gtcaaggctt 1380
cagttttctc aggccggagc gagtgacatt cgggaccagt ctaggaactg gcttcctgga 1440
ccctgttacc gccagcagcg agtatcaaag acatctgcgg ataacaacaa cagtgaatac 1500
tcgtggactg gagctaccaa gtaccacctc aatggcagag actctctggt gaatccgggc 1560
ccggccatgg caagccacaa ggacaatgaa gaaaagtttt ttcctcagag cggggttctc 1620
atctttggga agcaaggctc agagaaaaca aatgtggaca ttgaaaaggt catgattaca 1680
gacgaagagg aaatcaggac aaccaatccc gtggctacgg agcagtatgg ttctgtatct 1740
accaacctcc agagaggcaa cagacaagca gctaccgcag atgtcaacac acaaggcgtt 1800
cttccaggca tggtctggca ggacagagat gtgtaccttc aggggcccat ctgggcaaag 1860
attccacaca cggacggaca ttttcacccc tctcccctca tgggtggatt cggacctaaa 1920
caccctcctc cacagattct catcaagaac accccggtac ctgcgaatcc ttcgaccacc 1980
ttcagtgcgg caaagtttgc ttccttcatc acacagtact ccacgggaca ggtcagcgtg 2040
gagatcgagt gggagctgca gaaggaaaac agcaaacgct ggaatcccga aattcagtac 2100
acttccaact acaacaagtc tgttaatgtg gactttactg tggacactaa tggcgtgtat 2160
tcagagcctc gccccattgg caccagatac ctgactcgta atttgtaa 2208
<210>211
<211>2208
<212>DNA
<213>腺伴随病毒,血清型2
<400>211
atggctgccg atggttatct tccagattgg ctcgaggaca ctctctctga aggaataaga 60
cagtggtgga agctcaaacc tggcccacca ccaccaaagc ccgcagagcg gcataaggac 120
gacagcaggg gtcttgtgct tcctgggtac aagtacctcg gacccttcaa cggactcgac 180
aagggagagc cggtcaacga ggcagacgcc gcggccctcg agcacgacaa agcctacgac 240
cggcagctcg acagcggaga caacccgtac ctcaagtaca accacgccga cgcggagttt 300
caggagcgcc ttaaagaaga tacgtctttt gggggcaacc tcggacgagc agtcttccag 360
gcgaaaaaga gggttcttga acctctgggc ctggttgagg aacctgttaa gacggctccg 420
ggaaaaaaga ggccggtaga gcactctcct gtggagccag actcctcctc gggaaccgga 480
aaggcgggcc agcagcctgc aagaaaaaga ttgaattttg gtcagactgg agacgcagac 540
tcagtacctg acccccagcc tctcggacag ccaccagcag ccccctctgg tctgggaact 600
aatacgatgg ctacaggcag tggcgcacca atggcagaca ataacgaggg cgccgacgga 660
gtgggtaatt cctcgggaaa ttggcattgc gattccacat ggatgggcga cagagtcatc 720
accaccagca cccgaacctg ggccctgccc acctacaaca accacctcta caaacaaatt 780
tccagccaat caggagcctc gaacgacaat cactactttg gctacagcac cccttggggg 840
tattttgact tcaacagatt ccactgccac ttttcaccac gtgactggca aagactcatc 900
aacaacaact ggggattccg acccaagaga ctcaacttca agctctttaa cattcaagtc 960
aaagaggtca cgcagaatga cggtacgacg acgattgcca ataaccttac cagcacggtt 1020
caggtgttta ctgactcgga gtaccagctc ccgtacgtcc tcggctcggc gcatcaagga 1080
tgcctcccgc cgttcccagc agacgtcttc atggtgccac agtatggata cctcaccctg 1140
aacaacggga gtcaggcagt aggacgctct tcattttact gcctggagta ctttccttct 1200
cagatgctgc gtaccggaaa caactttacc ttcagctaca cttttgagga cgttcctttc 1260
cacagcagct acgctcacag ccagagtctg gaccgtctca tgaatcctct catcgaccag 1320
tacctgtatt acttgagcag aacaaacact ccaagtggaa ccaccacgca gtcaaggctt 1380
cagttttctc aggccggagc gagtgacatt cgggaccagt ctaggaactg gcttcctgga 1440
ccctgttacc gccagcagcg agtatcaaag acatctgcgg ataacaacaa cagtgaatac 1500
tcgtggactg gagctaccaa gtaccacctc aatggcagag actctctggt gaatccgggc 1560
ccggccatgg caagccacaa ggacgatgaa gaaaagtttt ttcctcagag cggggttctc 1620
atctttggga agcaaggctc agagaaaaca aatgtggaca ttgaaaaggt catgattaca 1680
gacgaagagg aaatcaggac aaccaatccc gtggctacgg agcagtatgg ttctgtatct 1740
accaacctcc agagaggcaa cagacaagca gctaccgcag atgtcaacac acaaggcgtt 1800
cttccaggca tggtctggca ggacagagat gtgtaccttc aggggcccat ctgggcaaag 1860
attccacaca cggacggaca ttttcacccc tctcccctca tgggtggatt cggacttaaa 1920
caccctcctc cacagattct catcaagaac accccggtac ctgcgaatcc ttcgaccacc 1980
ttcagtgcgg caaagtttgc ttccttcatc acacagtact ccacgggaca ggtcagcgtg 2040
gagatcgagt gggagctgca gaaggaaaac agcaaacgct ggaatcccga aattcagtac 2100
acttccaact acaacaagtc tgttaatgtg gactttactg tggacactaa tggcgtgtat 2160
tcagagcctc gccccattgg caccagatac ctgactcgta atctgtaa 2208
<210>212
<211>2208
<212>DNA
<213>新AAV血清型,克隆hu.64
<400>212
atggctgccg atggttatct tccagattgg ctcgaggaca ctctctctga aggaataaga 60
cagtggtgga agctcaaacc tggcccacca ccaccaaagc ccgcagagcg gcataaggac 120
gacagcaggg gtcttgtgct tcctgggtac aagtaccttg gacccttcaa cggactcgac 180
aagggagagc cggtcaacga ggcagacgcc gcggccctcg agcacgacaa ggcctacgac 240
cggcagctcg acggcggaga caacccgtac ctcaagtaca accacgccga cgcggagttc 300
caggagcgtc ttaaagaaga tacgtctttt gggggcaacc tcggacgagc agtcttccag 360
gcgaaaaaga gggttcttga acctctgggc ctggttgagg aacctgttaa gacggctccg 420
ggaaaaaaga ggccggtaga gcactctctt gcggagccag actcctcctc gggaaccgga 480
aaagcgggcc agcagcctgc aagaaggaga ttgaatttcg gtcagactgg agacgcagac 540
tccgtacctg acccccagcc tctcggacag ccaccagcag ccccctctgg tctgggaact 600
aatacgatgg ctacaggcag tggcgcacca atggcagaca ataacgaggg cgccgacgga 660
gtgggtaatt cctcgggaaa ttggcattgc gattccacat ggatgggcga cagagtcatc 720
accaccagca cccgcacctg ggctctgccc acctacaaca accacctcta caggcagatt 780
tccagccaat caggagcctc aaacgacaac cactactttg gctacagcac cccttggggg g40
tattttgact tcaacagatt ccactgccac ttttcgccac gtgactggca aagactcatc 900
aacaacaatt ggggatcccg gcccaaaaga ctcaacttca agctctttaa cattcaagtc 960
aaggaggtca cgcagaatga cggtacgacg acgattgcca ataaccttac cagcacggtt 1020
caggtgttta ctgactcgga gtaccagctc ccgtacgtcc tcggctcggc gcatcaagga 1080
tgcctcccgc cgttcccagc agacgtcttc atggtgccac agtatggata cctcaccctg 1140
aacaacggga gtcaggcagt aggacgctct tcattttact gcctggagta ctttccttct 1200
cagatgctgc gtaccggaaa caactttacc ttcagctaca cctttgagga cgttcctttc 1260
cacagcagct acgcccacag ccagagtttg gaccgtctca tgaatcctct cgtcgaccag 1320
tacctgtatt acttgagcag aacaaacact ccaagcggaa ccaccacgca gtcaaggctt 1380
cagttttctc aggccggagc aagtgacatt cgggaccagt ctaggaactg gcttcctgga 1440
ccctgttacc gccagcagcg agtatcaaag acatctgcgg ataacaacaa cagtgaatac 1500
tcgtggactg gagctaccaa gtaccacctt aatggaagag actctctggt gaatccgggc 1560
ccggccatgg ccagccacaa ggacgatgaa gaaaagtttt ttcctcagag cggggttctc 1620
atctttggaa aacaagactc gggaaaaact aatgtggaca ttgaaaaggt catgattaca 1680
gacgaagagg aaatcaggac caccaatccc gtggctacgg agcagtatgg ttctgtatct 1740
accaacctcc agagcggcaa cacacaagca gctacctcag atgtcaacac acaaggcgtt 1800
cttccaggca tggtgtggca ggacagagac gtgtacctgc aggggcccat ctgggcaaag 1860
attccacaca cggacggaca ttttcacccc tctcccctca tgggcggatt cggacttaaa 1920
caccctcccc cgcagattct catcaagaac accccggtac ctgcgaatcc ttcgactacc 1980
ttcagtgcgg caaagtttgc ttccttcatt acacagtact ccacggggca ggtcagcgtg 2040
gagatcgagt gggagctgca gaaggagaac agcaaacgct ggaatcccga gattcagtac 2100
acttccaact acaacaaatc tgttaatgtg gactttactg tggacactaa tggtgtgtat 2160
tcagagcctc gccccattgg caccagatac ctgactcgta atctgtaa 2208
<210>213
<211>2214
<212>DNA
<213>腺伴随病毒,血清型7
<400>213
atggctgccg atggttatct tccagattgg ctcgaggaca acctctctga gggcattcgc 60
gagtggtggg acctgaaacc tggagccccg aaacccaaag ccaaccagca aaagcaggac 120
aacggccggg gtctggtgct tcctggctac aagtacctcg gacccttcaa cggactcgac 180
aagggggagc ccgtcaacgc ggcggacgca gcggccctcg agcacgacaa ggcctacgac 240
cagcagctca aagcgggtga caatccgtac ctgcggtata accacgccga cgccgagttt 300
caggagcgtc tgcaagaaga tacgtcattt gggggcaacc tcgggcgagc agtcttccag 360
gccaagaagc gggttctcga acctctcggt ctggttgagg aaggcgctaa gacggctcct 420
gcaaagaaga gaccggtaga gccgtcacct cagcgttccc ccgactcctc cacgggcatc 480
ggcaagaaag gccagcagcc cgccagaaag agactcaatt tcggtcagac tggcgactca 540
gagtcagtcc ccgaccctca acctctcgga gaacctccag cagcgccctc tagtgtggga 600
tctggtacag tggctgcagg cggtggcgca ccaatggcag acaataacga aggtgccgac 660
ggagtgggta atgcctcagg aaattggcat tgcgattcca catggctggg cgacagagtc 720
attaccacca gcacccgaac ctgggccctg cccacctaca acaaccacct ctacaagcaa 780
atctccagtg aaactgcagg tagtaccaac gacaacacct acttcggcta cagcaccccc 840
tgggggtatt ttgactttaa cagattccac tgccacttct caccacgtga ctggcagcga 900
ctcatcaaca acaactgggg attccggccc aagaagctgc ggttcaagct cttcaacatc 960
caggtcaagg aggtcacgac gaatgacggc gttacgacca tcgctaataa ccttaccagc 1020
acgattcagg tattctcgga ctcggaatac cagctgccgt acgtcctcgg ctctgcgcac 1080
cagggctgcc tgcctccgtt cccggcggac gtcttcatga ttcctcagta cggctacctg 1140
actctcaaca atggcagtca gtctgtggga cgttcctcct tctactgcct ggagtacttc 1200
ccctctcaga tgctgagaac gggcaacaac tttgagttca gctacagctt cgaggacgtg 1260
cctttccaca gcagctacgc acacagccag agcctggacc ggctgatgaa tcccctcatc 1320
gaccagtact tgtactacct ggccagaaca cagagtaacc caggaggcac agctggcaat 1380
cgggaactgc agttttacca gggcgggcct tcaactatgg ccgaacaagc caagaattgg 1440
ttacctggac cttgcttccg gcaacaaaga gtctccaaaa cgctggatca aaacaacaac 1500
agcaactttg cttggactgg tgccaccaaa tatcacctga acggcagaaa ctcgttggtt 1560
aatcccggcg tcgccatggc aactcacaag gacgacgagg accgcttttt cccatccagc 1620
ggagtcctga tttttggaaa aactggagca actaacaaaa ctacattgga aaatgtgtta 1680
atgacaaatg aagaagaaat tcgtcctact aatcctgtag ccacggaaga atacgggata 1740
gtcagcagca acttacaagc ggctaatact gcagcccaga cacaagttgt caacaaccag 1800
ggagccttac ctggcatggt ctggcagaac cgggacgtgt acctgcaggg tcccatctgg 1860
gccaagattc ctcacacgga tggcaacttt cacccgtctc ctttgatggg cggctttgga 1920
cttaaacatc cgcctcctca gatcctgatc aagaacactc ccgttcccgc taatcctccg 1980
gaggtgttta ctcctgccaa gtttgcttcg ttcatcacac agtacagcac cggacaagtc 2040
agcgtggaaa tcgagtggga gctgcagaag gaaaacagca agcgctggaa cccggagatt 2100
cagtacacct ccaactttga aaagcagact ggtgtggact ttgccgttga cagccagggt 2160
gtttactctg agcctcgccc tattggcact cgttacctca cccgtaatct gtaa 2214
<210>214
<211>2217
<212>DNA
<213>腺伴随病毒,血清型8
<400>214
atggctgccg atggttatct tccagattgg ctcgaggaca acctctctga gggcattcgc 60
gagtggtggg cgctgaaacc tggagccccg aagcccaaag ccaaccagca aaagcaggac 120
gacggccggg gtctggtgct tcctggctac aagtacctcg gacccttcaa cggactcgac 180
aagggggagc ccgtcaacgc ggcggacgca gcggccctcg agcacgacaa ggcctacgac 240
cagcagctgc aggcgggtga caatccgtac ctgcggtata accacgccga cgccgagttt 300
caggagcgtc tgcaagaaga tacgtctttt gggggcaacc tcgggcgagc agtcttccag 360
gccaagaagc gggttctcga acctctcggt ctggttgagg aaggcgctaa gacggctcct 420
ggaaagaaga gaccggtaga gccatcaccc cagcgttctc cagactcctc tacgggcatc 480
ggcaagaaag gccaacagcc cgccagaaaa agactcaatt ttggtcagac tggcgactca 540
gagtcagttc cagaccctca acctctcgga gaacctccag cagcgccctc tggtgtggga 600
cctaatacaa tggctgcagg cggtggcgca ccaatggcag acaataacga aggcgccgac 660
ggagtgggta gttcctcggg aaattggcat tgcgattcca catggctggg cgacagagtc 720
atcaccacca gcacccgaac ctgggccctg cccacctaca acaaccacct ctacaagcaa 780
atctccaacg ggacatcggg aggagccacc aacgacaaca cctacttcgg ctacagcacc 840
ccctgggggt attttgactt taacagattc cactgccact tttcaccacg tgactggcag 900
cgactcatca acaacaactg gggattccgg cccaagagac tcagcttcaa gctcttcaac 960
atccaggtca aggaggtcac gcagaatgaa ggcaccaaga ccatcgccaa taacctcacc 1020
agcaccatcc aggtgtttac ggactcggag taccagctgc cgtacgttct cggctctgcc 1080
caccagggct gcctgcctcc gttcccggcg gacgtgttca tgattcccca gtacggctac 1140
ctaacactca acaacggtag tcaggccgtg ggacgctcct ccttctactg cctggaatac 1200
tttccttcgc agatgctgag aaccggcaac aacttccagt ttacttacac cttcgaggac 1260
gtgcctttcc acagcagcta cgcccacagc cagagcttgg accggctgat gaatcctctg 1320
attgaccagt acctgtacta cttgtctcgg actcaaacaa caggaggcac ggcaaatacg 1380
cagactctgg gcttcagcca aggtgggcct aatacaatgg ccaatcaggc aaagaactgg 1440
ctgccaggac cctgttaccg ccaacaacgc gtctcaacga caaccgggca aaacaacaat 1500
agcaactttg cctggactgc tgggaccaaa taccatctga atggaagaaa ttcattggct 1560
aatcctggca tcgctatggc aacacacaaa gacgacgagg agcgtttttt tcccagtaac 1620
gggatcctga tttttggcaa acaaaatgct gccagagaca atgcggatta cagcgatgtc 1680
atgctcacca gcgaggaaga aatcaaaacc actaaccctg tggctacaga ggaatacggt 1740
atcgtggcag ataacttgca gcagcaaaac acggctcctc aaattggaac tgtcaacagc 1800
cagggggcct tacccggtat ggtctggcag aaccgggacg tgtacctgca gggtcccatc 1860
tgggccaaga ttcctcacac ggacggcaac ttccacccgt ctccgctgat gggcggcttt 1920
ggcctgaaac atcctccgcc tcagatcctg atcaagaaca cgcctgtacc tgcggatcct 1980
ccgaccacct tcaaccagtc aaagctgaac tctttcatca cgcaatacag caccggacag 2040
gtcagcgtgg aaattgaatg ggagctgcag aaggaaaaca gcaagcgctg gaaccccgag 2100
atccagtaca cctccaacta ctacaaatct acaagtgtgg actttgctgt taatacagaa 2160
ggcgtgtact ctgaaccccg ccccattggc acccgttacc tcacccgtaa tctgtaa 2217
<210>215
<211>2217
<212>DNA
<213>新AAV血清型,克隆hu.67
<400>215
atggctgctg acggttatct tccagattgg ctcgaggaca acctctctga gggcattcgc 60
gagtggtggg acctgaaacc tggagccccc aagcccaagg ccaaccagca gaagcaggac 120
gacggccggg gtctggtgct tcttggctac aagtacctcg gacccttcaa cggactcgac 180
aagggggagc ccgtcaatgc ggcggacgca gcggccctcg agcacgacaa ggcctacgac 240
cagcagctca aagcgggtga caatccgtac ctgcggtata accacgccga cgccgagttt 300
caggagcgtc tgcaagaaga tacgtctttt gggggcaacc tcgggcgagc agtcttccag 360
gccaagaagc gggttctcga acctctcggt ctggttgagg aagctgctaa gacggctcct 420
ggaaagaaga gaccggtaga accgtcacct cagcgttccc ccgactcctc cacgggcatc 480
ggcaagaaag gccagcagcc cgctaaaaag agactgaact ttggtcagac tggcgactca 540
gagtcagtcc ccgaccctca accaatcgga gaaccaccag caggcccctc tggtctggga 600
tctggtacaa tggctgcagg cggtggcgct ccaatggcag acaataacga aggcgccgac 660
ggagtgggta gttcctcagg aaattggcat tgcgattcca catggctggg cgacagagtc 720
atcaccacca gcacccgaac ctgggccctg cccacctaca acaaccacct ctacaagcaa 780
atatccaatg ggacatcggg aggaagcacc aacgacaaca cctacttcgg ctacagcacc 840
ccctgggggt attttgactt caacagattc cactgccact tctcaccacg tgactggcag 900
cgactcatca acaacaactg gggattccgg ccaaaaagac tcagcttcaa gctcttcaac 960
atccaggtca aggaggtcac gcagaatgaa ggcaccaaga ccatcgccaa taaccttacc 1020
agcacgattc aggtatttac ggactcggaa taccagctgc cgtacgtcct cggctccgcg 1080
caccagggct gcctgcctcc gttcccggcg gacgtcttca tgattcccca gtacggctac 1140
cttacactga acaatggaag tcaagccgta ggccgttcct ccttctactg cctggaatat 1200
tttccatctc aaatgctgcg aactggaaac aattttgaat tcagctacac cttcgaggac 1260
gtgcctttcc acagcggcta cgcacacagc cagagcttgg accgactgat gaatcctctc 1320
atcgaccagt acctgtacta cttatccaga actcagtcca caggaggaac tcaaggtacc 1380
cagcaattgt tattttctca agctgggcct gcaaacatgt cggctcaggc taagaactgg 1440
ctacctggac cttgctaccg gcagcagcga gtctctacga cactgtcgca aaacaacaac 1500
agcaactttg cttggactgg tgccaccaaa tatcacctga acggaagaga ctctttggta 1560
aatcccggtg tcgccatggc aacccacaag gacgacgagg aacgcttctt cccgtcgagt 1620
ggagtcctga tgtttggaaa acagggtgct ggaagagaca atgtggacta cagcagcgtt 1680
atgctaacca gcgaagaaga aattaaaacc actaaccctg tagccacaga acaatacggt 1740
gtggtggctg acaacttgca gcaaaccaat acagggccta ttgtgggaaa tgtcaacagc 1800
caaggagcct tacctggcat ggtctggcag aaccgagacg tgtacctgca gggtcccatc 1860
tgggccaaga ttcctcacac ggacggcaac ttccaccctt caccgctaat gggaggattt 1920
ggactgaagc acccacctcc tcagatcctg atcaagaaca cgccggtacc tgcggatcct 1980
ccaacgacgt tcagccaggc gaaattggct tccttcatta cgcagtacag caccggacag 2040
gtcagcgtgg aaatcgagtg ggagctgcag aaggagaaca gcaaacgctg gaacccagag 2100
attcagtaca cttcaaacta ctacaaatct acaaatgtgg actttgctgt caatacagag 2160
ggaacttatt ctgagcctcg ccccattggt actcgttacc tcacccgtaa tctgtaa 2217
<210>216
<211>724
<212>PRT
<213>vp1,血清型5
<400>216
Met Ser Phe Val Asp His Pro Pro Asp Trp Leu Glu Glu Val Gly Glu
1 5 10 15
Gly Leu Arg Glu Phe Leu Gly Leu Glu Ala Gly Pro Pro Lys Pro Lys
20 25 30
Pro Asn Gln Gln His Gln Asp Gln Ala Arg Gly Leu Val Leu Pro Gly
35 40 45
Tyr Asn Tyr Leu Gly Pro Gly Asn Gly Leu Asp Arg Gly Glu Pro Val
50 55 60
Asn Arg Ala Asp Glu Val Ala Arg Glu His Asp Ile Ser Tyr Asn Glu
65 70 75 80
Gln Leu Glu Ala Gly Asp Asn Pro Tyr Leu Lys Tyr Asn His Ala Asp
85 90 95
Ala Glu Phe Gln Glu Lys Leu Ala Asp Asp Thr Ser Phe Gly Gly Asn
100 105 110
Leu Gly Lys Ala Val Phe Gln Ala Lys Lys Arg Val Leu Glu Pro Phe
115 120 125
Gly Leu Val Glu Glu Gly Ala Lys Thr Ala Pro Thr Gly Lys Arg Ile
130 135 140
Asp Asp His Phe Pro Lys Arg Lys Lys Ala Arg Thr Glu Glu Asp Ser
145 150 155 160
Lys Pro Ser Thr Ser Ser Asp Ala Glu Ala Gly Pro Ser Gly Ser Gln
165 170 175
Gln Leu Gln Ile Pro Ala Gln Pro Ala Ser Ser Leu Gly Ala Asp Thr
180 185 190
Met Ser Ala Gly Gly Gly Gly Pro Leu Gly Asp Asn Asn Gln Gly Ala
195 200 205
Asp Gly Val Gly Asn Ala Ser Gly Asp Trp His Cys Asp Ser Thr Trp
210 215 220
Met Gly Asp Arg Val Val Thr Lys Ser Thr Arg Thr Trp Val Leu Pro
225 230 235 240
Ser Tyr Asn Asn His Gln Tyr Arg Glu Ile Lys Ser Gly Ser Val Asp
245 250 255
Gly Ser Asn Ala Asn Ala Tyr Phe Gly Tyr Ser Thr Pro Trp Gly Tyr
260 265 270
Phe Asp Phe Asn Arg Phe His Ser His Trp Ser Pro Arg Asp Trp Gln
275 280 285
Arg Leu Ile Asn Asn Tyr Trp Gly Phe Arg Pro Arg Ser Leu Arg Val
290 295 300
Lys Ile Phe Asn Ile Gln Val Lys Glu Val Thr Val Gln Asp Ser Thr
305 310 315 320
Thr Thr Ile Ala Asn Asn Leu Thr Ser Thr Val Gln Val Phe Thr Asp
325 330 335
Asp Asp Tyr Gln Leu Pro Tyr Val Val Gly Asn Gly Thr Glu Gly Cys
340 345 350
Leu Pro Ala Phe Pro Pro Gln Val Phe Thr Leu Pro Gln Tyr Gly Tyr
355 360 365
Ala Thr Leu Asn Arg Asp Asn Thr Glu Asn Pro Thr Glu Arg Ser Ser
370 375 380
Phe Phe Cys Leu Glu Tyr Phe Pro Ser Lys Met Leu Arg Thr Gly Asn
385 390 395 400
Asn Phe Glu Phe Thr Tyr Asn Phe Glu Glu Val Pro Phe His Ser Ser
405 410 415
Phe Ala Pro Ser Gln Asn Leu Phe Lys Leu Ala Asn Pro Leu Val Asp
420 425 430
Gln Tyr Leu Tyr Arg Phe Val Ser Thr Asn Asn Thr Gly Gly Val Gln
435 440 445
Phe Asn Lys Asn Leu Ala Gly Arg Tyr Ala Asn Thr Tyr Lys Asn Trp
450 455 460
Phe Pro Gly Pro Met Gly Arg Thr Gln Gly Trp Asn Leu Gly Ser Gly
465 470 475 480
Val Asn Arg Ala Ser Val Ser Ala Phe Ala Thr Thr Asn Arg Met Glu
485 490 495
Leu Glu Gly Ala Ser Tyr Gln Val Pro Pro Gln Pro Asn Gly Met Thr
500 505 510
Asn Asn Leu Gln Gly Ser Asn Thr Tyr Ala Leu Glu Asn Thr Met Ile
515 520 525
Phe Asn Ser Gln Pro Ala Asn Pro Gly Thr Thr Ala Thr Tyr Leu Glu
530 535 540
Gly Asn Met Leu Ile Thr Ser Glu Ser Glu Thr Gln Pro Val Asn Arg
545 550 555 560
Val Ala Tyr Asn Val Gly Gly Gln Met Ala Thr Asn Asn Gln Ser Ser
565 570 575
Thr Thr Ala Pro Ala Thr Gly Thr Tyr Asn Leu Gln Glu Ile Val Pro
580 585 590
Gly Ser Val Trp Met Glu Arg Asp Val Tyr Leu Gln Gly Pro Ile Trp
595 600 605
Ala Lys Ile Pro Glu Thr Gly Ala His Phe His Pro Ser Pro Ala Met
610 615 620
Gly Gly Phe Gly Leu Lys His Pro Pro Pro Met Met Leu Ile Lys Asn
625 630 635 640
Thr Pro Val Pro Gly Asn Ile Thr Ser Phe Ser Asp Val Pro Val Ser
645 650 655
Ser Phe Ile Thr Gln Tyr Ser Thr Gly Gln Val Thr Val Glu Met Glu
660 665 670
Trp Glu Leu Lys Lys Glu Asn Ser Lys Arg Trp Asn Pro Glu Ile Gln
675 680 685
Tyr Thr Asn Asn Tyr Asn Asp Pro Gln Phe Val Asp Phe Ala Pro Asp
690 695 700
Ser Thr Gly Glu Tyr Arg Thr Thr Arg Pro Ile Gly Thr Arg Tyr Leu
705 710 715 720
Thr Arg Pro Leu
<210>217
<211>736
<212>PRT
<213>vp1,血清型3-3
<400>217
Met Ala Ala Asp Gly Tyr Leu Pro Asp Trp Leu Glu Asp Asn Leu Ser
1 5 10 15
Glu Gly Ile Arg Glu Trp Trp Ala Leu Lys Pro Gly Val Pro Gln Pro
20 25 30
Lys Ala Asn Gln Gln His Gln Asp Asn Arg Arg Gly Leu Val Leu Pro
35 40 45
Gly Tyr Lys Tyr Leu Gly Pro Gly Asn Gly Leu Asp Lys Gly Glu Pro
50 55 60
Val Asn Glu Ala Asp Ala Ala Ala Leu Glu His Asp Lys Ala Tyr Asp
65 70 75 80
Gln Gln Leu Lys Ala Gly Asp Asn Pro Tyr Leu Lys Tyr Asn His Ala
85 90 95
Asp Ala Glu Phe Gln Glu Arg Leu Gln Glu Asp Thr Ser Phe Gly Gly
100 105 110
Asn Leu Gly Arg Ala Val Phe Gln Ala Lys Lys Arg Ile Leu Glu Pro
115 120 125
Leu Gly Leu Val Glu Glu Ala Ala Lys Thr Ala Pro Gly Lys Lys Gly
130 135 140
Ala Val Asp Gln Ser Pro Gln Glu Pro Asp Ser Ser Ser Gly Val Gly
145 150 155 160
Lys Ser Gly Lys Gln Pro Ala Arg Lys Arg Leu Asn Phe Gly Gln Thr
165 170 175
Gly Asp Ser Glu Ser Val Pro Asp Pro Gln Pro Leu Gly Glu Pro Pro
180 185 190
Ala Ala Pro Thr Ser Leu Gly Ser Asn Thr Met Ala Ser Gly Gly Gly
195 200 205
Ala Pro Met Ala Asp Asn Asn Glu Gly Ala Asp Gly Val Gly Asn Ser
210 215 220
Ser Gly Asn Trp His Cys Asp Ser Gln Trp Leu Gly Asp Arg Val Ile
225 230 235 240
Thr Thr Ser Thr Arg Thr Trp Ala Leu Pro Thr Tyr Asn Asn His Leu
245 250 255
Tyr Lys Gln Ile Ser Ser Gln Ser Gly Ala Ser Asn Asp Asn His Tyr
260 265 270
Phe Gly Tyr Ser Thr Pro Trp Gly Tyr Phe Asp Phe Asn Arg Phe His
275 280 285
Cys His Phe Ser Pro Arg Asp Trp Gln Arg Leu Ile Asn Asn Asn Trp
290 295 300
Gly Phe Arg Pro Lys Lys Leu Ser Phe Lys Leu Phe Asn Ile Gln Val
305 310 315 320
Arg Gly Val Thr Gln Asn Asp Gly Thr Thr Thr Ile Ala Asn Asn Leu
325 330 335
Thr Ser Thr Val Gln Val Phe Thr Asp Ser Glu Tyr Gln Leu Pro Tyr
340 345 350
Val Leu Gly Ser Ala His Gln Gly Cys Leu Pro Pro Phe Pro Ala Asp
355 360 365
Val Phe Met Val Pro Gln Tyr Gly Tyr Leu Thr Leu Asn Asn Gly Ser
370 375 380
Gln Ala Val Gly Arg Ser Ser Phe Tyr Cys Leu Glu Tyr Phe Pro Ser
385 390 395 400
Gln Met Leu Arg Thr Gly Asn Asn Phe Gln Phe Ser Tyr Thr Phe Glu
405 410 415
Asp Val Pro Phe His Ser Ser Tyr Ala His Ser Gln Ser Leu Asp Arg
420 425 430
Leu Met Asn Pro Leu Ile Asp Gln Tyr Leu Tyr Tyr Leu Asn Arg Thr
435 440 445
Gln Gly Thr Thr Ser Gly Thr Thr Asn Gln Ser Arg Leu Leu Phe Ser
450 455 460
Gln Ala Gly Pro Gln Ser Met Ser Leu Gln Ala Arg Asn Trp Leu Pro
465 470 475 480
Gly Pro Cys Tyr Arg Gln Gln Arg Leu Ser Lys Thr Ala Asn Asp Asn
485 490 495
Asn Asn Ser Asn Phe Pro Trp Thr Ala Ala Ser Lys Tyr His Leu Asn
500 505 510
Gly Arg Asp Ser Leu Val Asn Pro Gly Pro Ala Met Ala Ser His Lys
515 520 525
Asp Asp Glu Glu Lys Phe Phe Pro Met His Gly Asn Leu Ile Phe Gly
530 535 540
Lys Glu Gly Thr Thr Ala Ser Asn Ala Glu Leu Asp Asn Val Met Ile
545 550 555 560
Thr Asp Glu Glu Glu Ile Arg Thr Thr Asn Pro Val Ala Thr Glu Gln
565 570 575
Tyr Gly Thr Val Ala Asn Asn Leu Gln Ser Ser Asn Thr Ala Pro Thr
580 585 590
Thr Gly Thr Val Asn His Gln Gly Ala Leu Pro Gly Met Val Trp Gln
595 600 605
Asp Arg Asp Val Tyr Leu Gln Gly Pro Ile Trp Ala Lys Ile Pro His
610 615 620
Thr Asp Gly His Phe His Pro Ser Pro Leu Met Gly Gly Phe Gly Leu
625 630 635 640
Lys His Pro Pro Pro Gln Ile Met Ile Lys Asn Thr Pro Val Pro Ala
645 650 655
Asn Pro Pro Thr Thr Phe Ser Pro Ala Lys Phe Ala Ser Phe Ile Thr
660 665 670
Gln Tyr Ser Thr Gly Gln Val Ser Val Glu Ile Glu Trp Glu Leu Gln
675 680 685
Lys Glu Asn Ser Lys Arg Trp Asn Pro Glu Ile Gln Tyr Thr Ser Asn
690 695 700
Tyr Asn Lys Ser Val Asn Val Asp Phe Thr Val Asp Thr Asn Gly Val
705 710 715 720
Tyr Ser Glu Pro Arg Pro Ile Gly Thr Arg Tyr Leu Thr Arg Asn Leu
725 730 735
<210>218
<211>734
<212>PRT
<213>vp1,血清型4-4
<400>218
Met Thr Asp Gly Tyr Leu Pro Asp Trp Leu Glu Asp Asn Leu Ser Glu
1 5 10 15
Gly Val Arg Glu Trp Trp Ala Leu Gln Pro Gly Ala Pro Lys Pro Lys
20 25 30
Ala Asn Gln Gln His Gln Asp Asn Ala Arg Gly Leu Val Leu Pro Gly
35 40 45
Tyr Lys Tyr Leu Gly Pro Gly Asn Gly Leu Asp Lys Gly Glu Pro Val
50 55 60
Asn Ala Ala Asp Ala Ala Ala Leu Glu His Asp Lys Ala Tyr Asp Gln
65 70 75 80
Gln Leu Lys Ala Gly Asp Asn Pro Tyr Leu Lys Tyr Asn His Ala Asp
85 90 95
Ala Glu Phe Gln Gln Arg Leu Gln Gly Asp Thr Ser Phe Gly Gly Asn
100 105 110
Leu Gly Arg Ala Val Phe Gln Ala Lys Lys Arg Val Leu Glu Pro Leu
115 120 125
Gly Leu Val Glu Gln Ala Gly Glu Thr Ala Pro Gly Lys Lys Arg Pro
130 135 140
Leu Ile Glu Ser Pro Gln Gln Pro Asp Ser Ser Thr Gly Ile Gly Lys
145 150 155 160
Lys Gly Lys Gln Pro Ala Lys Lys Lys Leu Val Phe Glu Asp Glu Thr
165 170 175
Gly Ala Gly Asp Gly Pro Pro Glu Gly Ser Thr Ser Gly Ala Met Ser
180 185 190
Asp Asp Ser Glu Met Arg Ala Ala Ala Gly Gly Ala Ala Val Glu Gly
195 200 205
Gly Gln Gly Ala Asp Gly Val Gly Asn Ala Ser Gly Asp Trp His Cys
210 215 220
Asp Ser Thr Trp Ser Glu Gly His Val Thr Thr Thr Ser Thr Arg Thr
225 230 235 240
Trp Val Leu Pro Thr Tyr Asn Asn His Leu Tyr Lys Arg Leu Gly Glu
245 250 255
Ser Leu Gln Ser Asn Thr Tyr Asn Gly Phe Ser Thr Pro Trp Gly Tyr
260 265 270
Phe Asp Phe Asn Arg Phe His Cys His Phe Ser Pro Arg Asp Trp Gln
275 280 285
Arg Leu Ile Asn Asn Asn Trp Gly Met Arg Pro Lys Ala Met Arg Val
290 295 300
Lys Ile Phe Asn Ile Gln Val Lys Glu Val Thr Thr Ser Asn Gly Glu
305 310 315 320
Thr Thr Val Ala Asn Asn Leu Thr Ser Thr Val Gln Ile Phe Ala Asp
325 330 335
Ser Ser Tyr Glu Leu Pro Tyr Val Met Asp Ala Gly Gln Glu Gly Ser
340 345 350
Leu Pro Pro Phe Pro Asn Asp Val Phe Met Val Pro Gln Tyr Gly Tyr
355 360 365
Cys Gly Leu Val Thr Gly Asn Thr Ser Gln Gln Gln Thr Asp Arg Asn
370 375 380
Ala Phe Tyr Cys Leu Glu Tyr Phe Pro Ser Gln Met Leu Arg Thr Gly
385 390 395 400
Asn Asn Phe Glu Ile Thr Tyr Ser Phe Glu Lys Val Pro Phe His Ser
405 410 415
Met Tyr Ala His Ser Gln Ser Leu Asp Arg Leu Met Asn Pro Leu Ile
420 425 430
Asp Gln Tyr Leu Trp Gly Leu Gln Ser Thr Thr Thr Gly Thr Thr Leu
435 440 445
Asn Ala Gly Thr Ala Thr Thr Asn Phe Thr Lys Leu Arg Pro Thr Asn
450 455 460
Phe Ser Asn Phe Lys Lys Asn Trp Leu Pro Gly Pro Ser Ile Lys Gln
465 470 475 480
Gln Gly Phe Ser Lys Thr Ala Asn Gln Asn Tyr Lys Ile Pro Ala Thr
485 490 495
Gly Ser Asp Ser Leu Ile Lys Tyr Glu Thr His Ser Thr Leu Asp Gly
500 505 510
Arg Trp Ser Ala Leu Thr Pro Gly Pro Pro Met Ala Thr Ala Gly Pro
515 520 525
Ala Asp Ser Lys Phe Ser Asn Ser Gln Leu Ile Phe Ala Gly Pro Lys
530 535 540
Gln Asn Gly Asn Thr Ala Thr Val Pro Gly Thr Leu Ile Phe Thr Ser
545 550 555 560
Glu Glu Glu Leu Ala Ala Thr Asn Ala Thr Asp Thr Asp Met Trp Gly
565 570 575
Asn Leu Pro Gly Gly Asp Gln Ser Asn Ser Asn Leu Pro Thr Val Asp
580 585 590
Arg Leu Thr Ala Leu Gly Ala Val Pro Gly Met Val Trp Gln Asn Arg
595 600 605
Asp Ile Tyr Tyr Gln Gly Pro Ile Trp Ala Lys Ile Pro His Thr Asp
610 615 620
Gly His Phe His Pro Ser Pro Leu Ile Gly Gly Phe Gly Leu Lys His
625 630 635 640
Pro Pro Pro Gln Ile Phe Ile Lys Asn Thr Pro Val Pro Ala Asn Pro
645 650 655
Ala Thr Thr Phe Ser Ser Thr Pro Val Asn Ser Phe Ile Thr Gln Tyr
660 665 670
Ser Thr Gly Gln Val Ser Val Gln Ile Asp Trp Glu Ile Gln Lys Glu
675 680 685
Arg Ser Lys Arg Trp Asn Pro Glu Val Gln Phe Thr Ser Asn Tyr Gly
690 695 700
Gln Gln Asn Ser Leu Leu Trp Ala Pro Asp Ala Ala Gly Lys Tyr Thr
705 710 715 720
Glu Pro Arg Ala Ile Gly Thr Arg Tyr Leu Thr His His Leu
725 730
<210>219
<211>736
<212>PRT
<213>vp1,血清型1
<400>219
Met Ala Ala Asp Gly Tyr Leu Pro Asp Trp Leu Glu Asp Asn Leu Ser
1 5 10 15
Glu Gly Ile Arg Glu Trp Trp Asp Leu Lys Pro Gly Ala Pro Lys Pro
20 25 30
Lys Ala Asn Gln Gln Lys Gln Asp Asp Gly Arg Gly Leu Val Leu Pro
35 40 45
Gly Tyr Lys Tyr Leu Gly Pro Phe Asn Gly Leu Asp Lys Gly Glu Pro
50 55 60
Val Asn Ala Ala Asp Ala Ala Ala Leu Glu His Asp Lys Ala Tyr Asp
65 70 75 80
Gln Gln Leu Lys Ala Gly Asp Asn Pro Tyr Leu Arg Tyr Asn His Ala
85 90 95
Asp Ala Glu Phe Gln Glu Arg Leu Gln Glu Asp Thr Ser Phe Gly Gly
100 105 110
Asn Leu Gly Arg Ala Val Phe Gln Ala Lys Lys Arg Val Leu Glu Pro
115 120 125
Leu Gly Leu Val Glu Glu Gly Ala Lys Thr Ala Pro Gly Lys Lys Arg
130 135 140
Pro Val Glu Gln Ser Pro Gln Glu Pro Asp Ser Ser Ser Gly Ile Gly
145 150 155 160
Lys Thr Gly Gln Gln Pro Ala Lys Lys Arg Leu Asn Phe Gly Gln Thr
165 170 175
Gly Asp Ser Glu Ser Val Pro Asp Pro Gln Pro Leu Gly Glu Pro Pro
180 185 190
Ala Thr Pro Ala Ala Val Gly Pro Thr Thr Met Ala Ser Gly Gly Gly
195 200 205
Ala Pro Met Ala Asp Asn Asn Glu Gly Ala Asp Gly Val Gly Asn Ala
210 215 220
Ser Gly Asn Trp His Cys Asp Ser Thr Trp Leu Gly Asp Arg Val Ile
225 230 235 240
Thr Thr Ser Thr Arg Thr Trp Ala Leu Pro Thr Tyr Asn Asn His Leu
245 250 255
Tyr Lys Gln Ile Ser Ser Ala Ser Thr Gly Ala Ser Asn Asp Asn His
260 265 270
Tyr Phe Gly Tyr Ser Thr Pro Trp Gly Tyr Phe Asp Phe Asn Arg Phe
275 280 285
His Cys His Phe Ser Pro Arg Asp Trp Gln Arg Leu Ile Asn Asn Asn
290 295 300
Trp Gly Phe Arg Pro Lys Arg Leu Asn Phe Lys Leu Phe Asn Ile Gln
305 310 315 320
Val Lys Glu Val Thr Thr Asn Asp Gly Val Thr Thr Ile Ala Asn Asn
325 330 335
Leu Thr Ser Thr Val Gln Val Phe Ser Asp Ser Glu Tyr Gln Leu Pro
340 345 350
Tyr Val Leu Gly Ser Ala His Gln Gly Cys Leu Pro Pro Phe Pro Ala
355 360 365
Asp Val Phe Met Ile Pro Gln Tyr Gly Tyr Leu Thr Leu Asn Asn Gly
370 375 380
Ser Gln Ala Val Gly Arg Ser Ser Phe Tyr Cys Leu Glu Tyr Phe Pro
385 390 395 400
Ser Gln Met Leu Arg Thr Gly Asn Asn Phe Thr Phe Ser Tyr Thr Phe
405 410 415
Glu Glu Val Pro Phe His Ser Ser Tyr Ala His Ser Gln Ser Leu Asp
420 425 430
Arg Leu Met Asn Pro Leu Ile Asp Gln Tyr Leu Tyr Tyr Leu Asn Arg
435 440 445
Thr Gln Asn Gln Ser Gly Ser Ala Gln Asn Lys Asp Leu Leu Phe Ser
450 455 460
Arg Gly Ser Pro Ala Gly Met Ser Val Gln Pro Lys Asn Trp Leu Pro
465 470 475 480
Gly Pro Cys Tyr Arg Gln Gln Arg Val Ser Lys Thr Lys Thr Asp Asn
485 490 495
Asn Asn Ser Asn Phe Thr Trp Thr Gly Ala Ser Lys Tyr Asn Leu Asn
500 505 510
Gly Arg Glu Ser Ile Ile Asn Pro Gly Thr Ala Met Ala Ser His Lys
515 520 525
Asp Asp Glu Asp Lys Phe Phe Pro Met Ser Gly Val Met Ile Phe Gly
530 535 540
Lys Glu Ser Ala Gly Ala Ser Asn Thr Ala Leu Asp Asn Val Met Ile
545 550 555 560
Thr Asp Glu Glu Glu Ile Lys Ala Thr Asn Pro Val Ala Thr Glu Arg
565 570 575
Phe Gly Thr Val Ala Val Asn Phe Gln Ser Ser Ser Thr Asp Pro Ala
580 585 590
Thr Gly Asp Val His Ala Met Gly Ala Leu Pro Gly Met Val Trp Gln
595 600 605
Asp Arg Asp Val Tyr Leu Gln Gly Pro Ile Trp Ala Lys Ile Pro His
610 615 620
Thr Asp Gly His Phe His Pro Ser Pro Leu Met Gly Gly Phe Gly Leu
625 630 635 640
Lys Asn Pro Pro Pro Gln Ile Leu Ile Lys Asn Thr Pro Val Pro Ala
645 650 655
Asn Pro Pro Ala Glu Phe Ser Ala Thr Lys Phe Ala Ser Phe Ile Thr
660 665 670
Gln Tyr Ser Thr Gly Gln Val Ser Val Glu Ile Glu Trp Glu Leu Gln
675 680 685
Lys Glu Asn Ser Lys Arg Trp Asn Pro Glu Val Gln Tyr Thr Ser Asn
690 695 700
Tyr Ala Lys Ser Ala Asn Val Asp Phe Thr Val Asp Asn Asn Gly Leu
705 710 715 720
Tyr Thr Glu Pro Arg Pro Ile Gly Thr Arg Tyr Leu Thr Arg Pro Leu
725 730 735
<210>220
<211>736
<212>PRT
<213>vp1,血清型6
<400>220
Met Ala Ala Asp Gly Tyr Leu Pro Asp Trp Leu Glu Asp Asn Leu Ser
1 5 10 15
Glu Gly Ile Arg Glu Trp Trp Asp Leu Lys Pro Gly Ala Pro Lys Pro
20 25 30
Lys Ala Asn Gln Gln Lys Gln Asp Asp Gly Arg Gly Leu Val Leu Pro
35 40 45
Gly Tyr Lys Tyr Leu Gly Pro Phe Asn Gly Leu Asp Lys Gly Glu Pro
50 55 60
Val Asn Ala Ala Asp Ala Ala Ala Leu Glu His Asp Lys Ala Tyr Asp
65 70 75 80
Gln Gln Leu Lys Ala Gly Asp Asn Pro Tyr Leu Arg Tyr Asn His Ala
85 90 95
Asp Ala Glu Phe Gln Glu Arg Leu Gln Glu Asp Thr Ser Phe Gly Gly
100 105 110
Asn Leu Gly Arg Ala Val Phe Gln Ala Lys Lys Arg Val Leu Glu Pro
115 120 125
Phe Gly Leu Val Glu Glu Gly Ala Lys Thr Ala Pro Gly Lys Lys Arg
130 135 140
Pro Val Glu Gln Ser Pro Gln Glu Pro Asp Ser Ser Ser Gly Ile Gly
145 150 155 160
Lys Thr Gly Gln Gln Pro Ala Lys Lys Arg Leu Asn Phe Gly Gln Thr
165 170 175
Gly Asp Ser Glu Ser Val Pro Asp Pro Gln Pro Leu Gly Glu Pro Pro
180 185 190
Ala Thr Pro Ala Ala Val Gly Pro Thr Thr Met Ala Ser Gly Gly Gly
195 200 205
Ala Pro Met Ala Asp Asn Asn Glu Gly Ala Asp Gly Val Gly Asn Ala
210 215 220
Ser Gly Asn Trp His Cys Asp Ser Thr Trp Leu Gly Asp Arg Val Ile
225 230 235 240
Thr Thr Ser Thr Arg Thr Trp Ala Leu Pro Thr Tyr Asn Asn His Leu
245 250 255
Tyr Lys Gln Ile Ser Ser Ala Ser Thr Gly Ala Ser Asn Asp Asn His
260 265 270
Tyr Phe Gly Tyr Ser Thr Pro Trp Gly Tyr Phe Asp Phe Asn Arg Phe
275 280 285
His Cys His Phe Ser Pro Arg Asp Trp Gln Arg Leu Ile Asn Asn Asn
290 295 300
Trp Gly Phe Arg Pro Lys Arg Leu Asn Phe Lys Leu Phe Asn Ile Gln
305 310 315 320
Val Lys Glu Val Thr Thr Asn Asp Gly Val Thr Thr Ile Ala Asn Asn
325 330 335
Leu Thr Ser Thr Val Gln Val Phe Ser Asp Ser Glu Tyr Gln Leu Pro
340 345 350
Tyr Val Leu Gly Ser Ala His Gln Gly Cys Leu Pro Pro Phe Pro Ala
355 360 365
Asp Val Phe Met Ile Pro Gln Tyr Gly Tyr Leu Thr Leu Asn Asn Gly
370 375 380
Ser Gln Ala Val Gly Arg Ser Ser Phe Tyr Cys Leu Glu Tyr Phe Pro
385 390 395 400
Ser Gln Met Leu Arg Thr Gly Asn Asn Phe Thr Phe Ser Tyr Thr Phe
405 410 415
Glu Asp Val Pro Phe His Ser Ser Tyr Ala His Ser Gln Ser Leu Asp
420 425 430
Arg Leu Met Asn Pro Leu Ile Asp Gln Tyr Leu Tyr Tyr Leu Asn Arg
435 440 445
Thr Gln Asn Gln Ser Gly Ser Ala Gln Asn Lys Asp Leu Leu Phe Ser
450 455 460
Arg Gly Ser Pro Ala Gly Met Ser Val Gln Pro Lys Asn Trp Leu Pro
465 470 475 480
Gly Pro Cys Tyr Arg Gln Gln Arg Val Ser Lys Thr Lys Thr Asp Asn
485 490 495
Asn Asn Ser Asn Phe Thr Trp Thr Gly Ala Ser Lys Tyr Asn Leu Asn
500 505 510
Gly Arg Glu Ser Ile Ile Asn Pro Gly Thr Ala Met Ala Ser His Lys
515 520 525
Asp Asp Lys Asp Lys Phe Phe Pro Met Ser Gly Val Met Ile Phe Gly
530 535 540
Lys Glu Ser Ala Gly Ala Ser Asn Thr Ala Leu Asp Asn Val Met Ile
545 550 555 560
Thr Asp Glu Glu Glu Ile Lys Ala Thr Asn Pro Val Ala Thr Glu Arg
565 570 575
Phe Gly Thr Val Ala Val Asn Leu Gln Ser Ser Ser Thr Asp Pro Ala
580 585 590
Thr Gly Asp Val His Val Met Gly Ala Leu Pro Gly Met Val Trp Gln
595 600 605
Asp Arg Asp Val Tyr Leu Gln Gly Pro Ile Trp Ala Lys Ile Pro His
610 615 620
Thr Asp Gly His Phe His Pro Ser Pro Leu Met Gly Gly Phe Gly Leu
625 630 635 640
Lys His Pro Pro Pro Gln Ile Leu Ile Lys Asn Thr Pro Val Pro Ala
645 650 655
Asn Pro Pro Ala Glu Phe Ser Ala Thr Lys Phe Ala Ser Phe Ile Thr
660 665 670
Gln Tyr Ser Thr Gly Gln Val Ser Val Glu Ile Glu Trp Glu Leu Gln
675 680 685
Lys Glu Asn Ser Lys Arg Trp Asn Pro Glu Val Gln Tyr Thr Ser Asn
690 695 700
Tyr Ala Lys Ser Ala Asn Val Asp Phe Thr Val Asp Asn Asn Gly Leu
705 710 715 720
Tyr Thr Glu Pro Arg Pro Ile Gly Thr Arg Tyr Leu Thr Arg Pro Leu
725 730 735<210>221<211>735<212>PRT<213>vp1,血清型2<400>221
Met Ala Ala Asp Gly Tyr Leu Pro Asp Trp Leu Glu Asp Thr Leu Ser
1 5 10 15
Glu Gly Ile Arg Gln Trp Trp Lys Leu Lys Pro Gly Pro Pro Pro Pro
20 25 30
Lys Pro Ala Glu Arg His Lys Asp Asp Ser Arg Gly Leu Val Leu Pro
35 40 45
Gly Tyr Lys Tyr Leu Gly Pro Phe Asn Gly Leu Asp Lys Gly Glu Pro
50 55 60
Val Asn Glu Ala Asp Ala Ala Ala Leu Glu His Asp Lys Ala Tyr Asp
65 70 75 80
Arg Gln Leu Asp Ser Gly Asp Asn Pro Tyr Leu Lys Tyr Asn His Ala
85 90 95
Asp Ala Glu Phe Gln Glu Arg Leu Lys Glu Asp Thr Ser Phe Gly Gly
100 105 110
Asn Leu Gly Arg Ala Val Phe Gln Ala Lys Lys Arg Val Leu Glu Pro
115 120 125
Leu Gly Leu Val Glu Glu Pro Val Lys Thr Ala Pro Gly Lys Lys Arg
130 135 140
Pro Val Glu His Ser Pro Val Glu Pro Asp Ser Ser Ser Gly Thr Gly
145 150 155 160
Lys Ala Gly Gln Gln Pro Ala Arg Lys Arg Leu Asn Phe Gly Gln Thr
165 170 175
Gly Asp Ala Asp Ser Val Pro Asp Pro Gln Pro Leu Gly Gln Pro Pro
180 185 190
Ala Ala Pro Ser Gly Leu Gly Thr Asn Thr Met Ala Thr Gly Ser Gly
195 200 205
Ala Pro Met Ala Asp Asn Asn Glu Gly Ala Asp Gly Val Gly Asn Ser
210 215 220
Ser Gly Asn Trp His Cys Asp Ser Thr Trp Met Gly Asp Arg Val Ile
225 230 235 240
Thr Thr Ser Thr Arg Thr Trp Ala Leu Pro Thr Tyr Asn Asn His Leu
245 250 255
Tyr Lys Gln Ile Ser Ser Gln Ser Gly Ala Ser Asn Asp Asn His Tyr
260 265 270
Phe Gly Tyr Ser Thr Pro Trp Gly Tyr Phe Asp Phe Asn Arg Phe His
275 280 285
Cys His Phe Ser Pro Arg Asp Trp Gln Arg Leu Ile Asn Asn Asn Trp
290 295 300
Gly Phe Arg Pro Lys Arg Leu Asn Phe Lys Leu Phe Asn Ile Gln Val
305 310 315 320
Lys Glu Val Thr Gln Asn Asp Gly Thr Thr Thr Ile Ala Asn Asn Leu
325 330 335
Thr Ser Thr Val Gln Val Phe Thr Asp Ser Glu Tyr Gln Leu Pro Tyr
340 345 350
Val Leu Gly Ser Ala His Gln Gly Cys Leu Pro Pro Phe Pro Ala Asp
355 360 365
Val Phe Met Val Pro Gln Tyr Gly Tyr Leu Thr Leu Asn Asn Gly Ser
370 375 380
Gln Ala Val Gly Arg Ser Ser Phe Tyr Cys Leu Glu Tyr Phe Pro Ser
385 390 395 400
Gln Met Leu Arg Thr Gly Asn Asn Phe Thr Phe Ser Tyr Thr Phe Glu
405 410 415
Asp Val Pro Phe His Ser Ser Tyr Ala His Ser Gln Ser Leu Asp Arg
420 425 430
Leu Met Asn Pro Leu Ile Asp Gln Tyr Leu Tyr Tyr Leu Ser Arg Thr
435 440 445
Asn Thr Pro Ser Gly Thr Thr Thr Gln Ser Arg Leu Gln Phe Ser Gln
450 455 460
Ala Gly Ala Ser Asp Ile Arg Asp Gln Ser Arg Asn Trp Leu Pro Gly
465 470 475 480
Pro Cys Tyr Arg Gln Gln Arg Val Ser Lys Thr Ser Ala Asp Asn Asn
485 490 495
Asn Ser Glu Tyr Ser Trp Thr Gly Ala Thr Lys Tyr His Leu Asn Gly
500 505 510
Arg Asp Ser Leu Val Asn Pro Gly Pro Ala Met Ala Ser His Lys Asp
515 520 525
Asp Glu Glu Lys Phe Phe Pro Gln Ser Gly Val Leu Ile Phe Gly Lys
530 535 540
Gln Gly Ser Glu Lys Thr Asn Val Asp Ile Glu Lys Val Met Ile Thr
545 550 555 560
Asp Glu Glu Glu Ile Arg Thr Thr Asn Pro Val Ala Thr Glu Gln Tyr
565 570 575
Gly Ser Val Ser Thr Asn Leu Gln Arg Gly Asn Arg Gln Ala Ala Thr
580 585 590
Ala Asp Val Asn Thr Gln Gly Val Leu Pro Gly Met Val Trp Gln Asp
595 600 605
Arg Asp Val Tyr Leu Gln Gly Pro Ile Trp Ala Lys Ile Pro His Thr
610 615 620
Asp Gly His Phe His Pro Ser Pro Leu Met Gly Gly Phe Gly Leu Lys
625 630 635 640
His Pro Pro Pro Gln Ile Leu Ile Lys Asn Thr Pro Val Pro Ala Asn
645 650 655
Pro Ser Thr Thr Phe Ser Ala Ala Lys Phe Ala Ser Phe Ile Thr Gln
660 665 670
Tyr Ser Thr Gly Gln Val Ser Val Glu Ile Glu Trp Glu Leu Gln Lys
675 680 685
Glu Asn Ser Lys Arg Trp Asn Pro Glu Ile Gln Tyr Thr Ser Asn Tyr
690 695 700
Asn Lys Ser Val Asn Val Asp Phe Thr Val Asp Thr Asn Gly Val Tyr
705 710 715 720
Ser Glu Pro Arg Pro Ile Gly Thr Arg Tyr Leu Thr Arg Asn Leu
725 730 735
<210>222
<211>737
<212>PRT
<213>vp1,血清型7
<400>222
Met Ala Ala Asp Gly Tyr Leu Pro Asp Trp Leu Glu Asp Asn Leu Ser
1 5 10 15
Glu Gly Ile Arg Glu Trp Trp Asp Leu Lys Pro Gly Ala Pro Lys Pro
20 25 30
Lys Ala Asn Gln Gln Lys Gln Asp Asn Gly Arg Gly Leu Val Leu Pro
35 40 45
Gly Tyr Lys Tyr Leu Gly Pro Phe Asn Gly Leu Asp Lys Gly Glu Pro
50 55 60
Val Asn Ala Ala Asp Ala Ala Ala Leu Glu His Asp Lys Ala Tyr Asp
65 70 75 80
Gln Gln Leu Lys Ala Gly Asp Asn Pro Tyr Leu Arg Tyr Asn His Ala
85 90 95
Asp Ala Glu Phe Gln Glu Arg Leu Gln Glu Asp Thr Ser Phe Gly Gly
100 105 110
Asn Leu Gly Arg Ala Val Phe Gln Ala Lys Lys Arg Val Leu Glu Pro
115 120 125
Leu Gly Leu Val Glu Glu Gly Ala Lys Thr Ala Pro Ala Lys Lys Arg
130 135 140
Pro Val Glu Pro Ser Pro Gln Arg Ser Pro Asp Ser Ser Thr Gly Ile
145 150 155 160
Gly Lys Lys Gly Gln Gln Pro Ala Arg Lys Arg Leu Asn Phe Gly Gln
165 170 175
Thr Gly Asp Ser Glu Ser Val Pro Asp Pro Gln Pro Leu Gly Glu Pro
180 185 190
Pro Ala Ala Pro Ser Ser Val Gly Ser Gly Thr Val Ala Ala Gly Gly
195 200 205
Gly Ala Pro Met Ala Asp Asn Asn Glu Gly Ala Asp Gly Val Gly Asn
210 215 220
Ala Ser Gly Asn Trp His Cys Asp Ser Thr Trp Leu Gly Asp Arg Val
225 230 235 240
Ile Thr Thr Ser Thr Arg Thr Trp Ala Leu Pro Thr Tyr Asn Asn His
245 250 255
Leu Tyr Lys Gln Ile Ser Ser Glu Thr Ala Gly Ser Thr Asn Asp Asn
260 265 270
Thr Tyr Phe Gly Tyr Ser Thr Pro Trp Gly Tyr Phe Asp Phe Asn Arg
275 280 285
Phe His Cys His Phe Ser Pro Arg Asp Trp Gln Arg Leu Ile Asn Asn
290 295 300
Asn Trp Gly Phe Arg Pro Lys Lys Leu Arg Phe Lys Leu Phe Asn Ile
305 310 315 320
Gln Val Lys Glu Val Thr Thr Asn Asp Gly Val Thr Thr Ile Ala Asn
325 330 335
Asn Leu Thr Ser Thr Ile Gln Val Phe Ser Asp Ser Glu Tyr Gln Leu
340 345 350
Pro Tyr Val Leu Gly Ser Ala His Gln Gly Cys Leu Pro Pro Phe Pro
355 360 365
Ala Asp Val Phe Met Ile Pro Gln Tyr Gly Tyr Leu Thr Leu Asn Asn
370 375 380
Gly Ser Gln Ser Val Gly Arg Ser Ser Phe Tyr Cys Leu Glu Tyr Phe
385 390 395 400
Pro Ser Gln Met Leu Arg Thr Gly Asn Asn Phe Glu Phe Ser Tyr Ser
405 410 415
Phe Glu Asp Val Pro Phe His Ser Ser Tyr Ala His Ser Gln Ser Leu
420 425 430
Asp Arg Leu Met Asn Pro Leu Ile Asp Gln Tyr Leu Tyr Tyr Leu Ala
435 440 445
Arg Thr Gln Ser Asn Pro Gly Gly Thr Ala Gly Asn Arg Glu Leu Gln
450 455 460
Phe Tyr Gln Gly Gly Pro Ser Thr Met Ala Glu Gln Ala Lys Asn Trp
465 470 475 480
Leu Pro Gly Pro Cys Phe Arg Gln Gln Arg Val Ser Lys Thr Leu Asp
485 490 495
Gln Asn Asn Asn Ser Asn Phe Ala Trp Thr Gly Ala Thr Lys Tyr His
500 505 510
Leu Asn Gly Arg Asn Ser Leu Val Asn Pro Gly Val Ala Met Ala Thr
515 520 525
His Lys Asp Asp Glu Asp Arg Phe Phe Pro Ser Ser Gly Val Leu Ile
530 535 540
Phe Gly Lys Thr Gly Ala Thr Asn Lys Thr Thr Leu Glu Asn Val Leu
545 550 555 560
Met Thr Asn Glu Glu Glu Ile Arg Pro Thr Asn Pro Val Ala Thr Glu
565 570 575
Glu Tyr Gly Ile Val Ser Ser Asn Leu Gln Ala Ala Asn Thr Ala Ala
580 585 590
Gln Thr Gln Val Val Asn Asn Gln Gly Ala Leu Pro Gly Met Val Trp
595 600 605
Gln Asn Arg Asp Val Tyr Leu Gln Gly Pro Ile Trp Ala Lys Ile Pro
610 615 620
His Thr Asp Gly Asn Phe His Pro Ser Pro Leu Met Gly Gly Phe Gly
625 630 635 640
Leu Lys His Pro Pro Pro Gln Ile Leu Ile Lys Asn Thr Pro Val Pro
645 650 655
Ala Asn Pro Pro Glu Val Phe Thr Pro Ala Lys Phe Ala Ser Phe Ile
660 665 670
Thr Gln Tyr Ser Thr Gly Gln Val Ser Val Glu Ile Glu Trp Glu Leu
675 680 685
Gln Lys Glu Asn Ser Lys Arg Trp Asn Pro Glu Ile Gln Tyr Thr Ser
690 695 700
Asn Phe Glu Lys Gln Thr Gly Val Asp Phe Ala Val Asp Ser Gln Gly
705 710 715 720
Val Tyr Ser Glu Pro Arg Pro Ile Gly Thr Arg Tyr Leu Thr Arg Asn
725 730 735
Leu
<210>223
<211>738
<212>PRT
<213>vp1,血清型8
<400>223
Met Ala Ala Asp Gly Tyr Leu Pro Asp Trp Leu Glu Asp Asn Leu Ser
1 5 10 15
Glu Gly Ile Arg Glu Trp Trp Ala Leu Lys Pro Gly Ala Pro Lys Pro
20 25 30
Lys Ala Asn Gln Gln Lys Gln Asp Asp Gly Arg Gly Leu Val Leu Pro
35 40 45
Gly Tyr Lys Tyr Leu Gly Pro Phe Asn Gly Leu Asp Lys Gly Glu Pro
50 55 60
Val Asn Ala Ala Asp Ala Ala Ala Leu Glu His Asp Lys Ala Tyr Asp
65 70 75 80
Gln Gln Leu Gln Ala Gly Asp Asn Pro Tyr Leu Arg Tyr Asn His Ala
85 90 95
Asp Ala Glu Phe Gln Glu Arg Leu Gln Glu Asp Thr Ser Phe Gly Gly
100 105 110
Asn Leu Gly Arg Ala Val Phe Gln Ala Lys Lys Arg Val Leu Glu Pro
115 120 125
Leu Gly Leu Val Glu Glu Gly Ala Lys Thr Ala Pro Gly Lys Lys Arg
130 135 140
Pro Val Glu Pro Ser Pro Gln Arg Ser Pro Asp Ser Ser Thr Gly Ile
145 150 155 160
Gly Lys Lys Gly Gln Gln Pro Ala Arg Lys Arg Leu Asn Phe Gly Gln
165 170 175
Thr Gly Asp Ser Glu Ser Val Pro Asp Pro Gln Pro Leu Gly Glu Pro
180 185 190
Pro Ala Ala Pro Ser Gly Val Gly Pro Asn Thr Met Ala Ala Gly Gly
195 200 205
Gly Ala Pro Met Ala Asp Asn Asn Glu Gly Ala Asp Gly Val Gly Ser
210 215 220
Ser Ser Gly Asn Trp His Cys Asp Ser Thr Trp Leu Gly Asp Arg Val
225 230 235 240
Ile Thr Thr Ser Thr Arg Thr Trp Ala Leu Pro Thr Tyr Asn Asn His
245 250 255
Leu Tyr Lys Gln Ile Ser Asn Gly Thr Ser Gly Gly Ala Thr Asn Asp
260 265 270
Asn Thr Tyr Phe Gly Tyr Ser Thr Pro Trp Gly Tyr Phe Asp Phe Asn
275 280 285
Arg Phe His Cys His Phe Ser Pro Arg Asp Trp Gln Arg Leu Ile Asn
290 295 300
Asn Asn Trp Gly Phe Arg Pro Lys Arg Leu Ser Phe Lys Leu Phe Asn
305 310 315 320
Ile Gln Val Lys Glu Val Thr Gln Asn Glu Gly Thr Lys Thr Ile Ala
325 330 335
Asn Asn Leu Thr Ser Thr Ile Gln Val Phe Thr Asp Ser Glu Tyr Gln
340 345 350
Leu Pro Tyr Val Leu Gly Ser Ala His Gln Gly Cys Leu Pro Pro Phe
355 360 365
Pro Ala Asp Val Phe Met Ile Pro Gln Tyr Gly Tyr Leu Thr Leu Asn
370 375 380
Asn Gly Ser Gln Ala Val Gly Arg Ser Ser Phe Tyr Cys Leu Glu Tyr
385 390 395 400
Phe Pro Ser Gln Met Leu Arg Thr Gly Asn Asn Phe Gln Phe Thr Tyr
405 410 415
Thr Phe Glu Asp Val Pro Phe His Ser Ser Tyr Ala His Ser Gln Ser
420 425 430
Leu Asp Arg Leu Met Asn Pro Leu Ile Asp Gln Tyr Leu Tyr Tyr Leu
435 440 445
Ser Arg Thr Gln Thr Thr Gly Gly Thr Ala Asn Thr Gln Thr Leu Gly
450 455 460
Phe Ser Gln Gly Gly Pro Asn Thr Met Ala Asn Gln Ala Lys Asn Trp
465 470 475 480
Leu Pro Gly Pro Cys Tyr Arg Gln Gln Arg Val Ser Thr Thr Thr Gly
485 490 495
Gln Asn Asn Asn Ser Asn Phe Ala Trp Thr Ala Gly Thr Lys Tyr His
500 505 510
Leu Asn Gly Arg Asn Ser Leu Ala Asn Pro Gly Ile Ala Met Ala Thr
515 520 525
His Lys Asp Asp Glu Glu Arg Phe Phe Pro Ser Asn Gly Ile Leu Ile
530 535 540
Phe Gly Lys Gln Asn Ala Ala Arg Asp Asn Ala Asp Tyr Ser Asp Val
545 550 555 560
Met Leu Thr Ser Glu Glu Glu Ile Lys Thr Thr Asn Pro Val Ala Thr
565 570 575
Glu Glu Tyr Gly Ile Val Ala Asp Asn Leu Gln Gln Gln Asn Thr Ala
580 585 590
Pro Gln Ile Gly Thr Val Asn Ser Gln Gly Ala Leu Pro Gly Met Val
595 600 605
Trp Gln Asn Arg Asp Val Tyr Leu Gln Gly Pro Ile Trp Ala Lys Ile
610 615 620
Pro His Thr Asp Gly Asn Phe His Pro Ser Pro Leu Met Gly Gly Phe
625 630 635 640
Gly Leu Lys His Pro Pro Pro Gln Ile Leu Ile Lys Asn Thr Pro Val
645 650 655
Pro Ala Asp Pro Pro Thr Thr Phe Asn Gln Ser Lys Leu Asn Ser Phe
660 665 670
Ile Thr Gln Tyr Ser Thr Gly Gln Val Ser Val Glu Ile Glu Trp Glu
675 680 685
Leu Gln Lys Glu Asn Ser Lys Arg Trp Asn Pro Glu Ile Gln Tyr Thr
690 695 700
Ser Asn Tyr Tyr Lys Ser Thr Ser Val Asp Phe Ala Val Asn Thr Glu
705 710 715 720
Gly Val Tyr Ser Glu Pro Arg Pro Ile Gly Thr Arg Tyr Leu Thr Arg
725 730 735
Asn Leu
<210>224
<211>736
<212>PRT
<213>vp1,修饰的hu.46
<400>224
Met Ala Ala Asp Gly Tyr Leu Pro Asp Trp Leu Glu Asp Thr Leu Ser
1 5 10 15
Glu Gly Ile Arg Gln Trp Trp Lys Leu Lys Pro Gly Pro Pro Pro Pro
20 25 30
Lys Pro Ala Glu Arg His Lys Asp Asp Ser Arg Gly Leu Val Leu Pro
35 40 45
Gly Tyr Lys Tyr Leu Gly Pro Phe Asn Gly Leu Asp Lys Gly Glu Pro
50 55 60
Val Asn Glu Ala Asp Ala Ala Ala Leu Glu His Asp Lys Ala Tyr Asp
65 70 75 80
Arg Gln Leu Asp Ser Gly Asp Asn Pro Tyr Leu Lys Tyr Asn His Ala
85 90 95
Asp Ala Glu Phe Gln Glu Arg Leu Lys Glu Asp Thr Ser Phe Gly Gly
100 105 110
Asn Leu Gly Arg Ala Val Phe Gln Ala Lys Lys Arg Val Leu Glu Pro
115 120 125
Leu Gly Leu Val Glu Glu Gly Ala Lys Thr Ala Pro Gly Lys Lys Arg
130 135 140
Pro Val Glu Gln Ser Pro Gln Glu Pro Asp Ser Ser Ser Gly Ile Gly
145 150 155 160
Lys Thr Gly Gln Gln Pro Ala Lys Lys Arg Leu Asn Phe Gly Gln Thr
165 170 175
Gly Asp Ser Glu Ser Val Pro Asp Pro Gln Pro Leu Gly Glu Pro Pro
180 185 190
Ala Thr Pro Ala Ala Val Gly Pro Thr Thr Met Ala Ser Gly Gly Gly
195 200 205
Ala Pro Met Ala Asp Asn Asn Glu Gly Ala Asp Gly Val Gly Asn Ala
210 215 220
Ser Gly Asn Trp His Cys Asp Ser Thr Trp Leu Gly Asp Arg Val Ile
225 230 235 240
Thr Thr Ser Thr Arg Thr Trp Ala Leu Pro Thr Tyr Asn Asn His Leu
245 250 255
Tyr Lys Gln Ile Ser Ser Ala Ser Thr Gly Ala Ser Asn Asp Asn His
260 265 270
Tyr Phe Gly Tyr Ser Thr Pro Trp Gly Tyr Phe Asp Phe Asn Arg Phe
275 280 285
His Cys His Phe Ser Pro Arg Asp Trp Gln Arg Leu Ile Asn Asn Asn
290 295 300
Trp Gly Phe Arg Pro Lys Arg Leu Asn Phe Lys Leu Phe Asn Ile Gln
305 310 315 320
Val Lys Glu Val Thr Thr Asn Asp Gly Val Thr Thr Ile Ala Asn Asn
325 330 335
Leu Thr Ser Thr Val Gln Val Phe Ser Asp Ser Glu Tyr Gln Leu Pro
340 345 350
Tyr Val Leu Gly Ser Ala His Gln Gly Cys Leu Pro Pro Phe Pro Ala
355 360 365
Asp Val Phe Met Ile Pro Gln Tyr Gly Tyr Leu Thr Leu Asn Asn Gly
370 375 380
Ser Gln Ala Val Gly Arg Ser Ser Phe Tyr Cys Leu Glu Tyr Phe Pro
385 390 395 400
Ser Gln Met Leu Arg Thr Gly Asn Asn Phe Thr Phe Ser Tyr Thr Phe
405 410 415
Glu Glu Val Pro Leu His Ser Ser Cys Ala His Ser Gln Ser Leu Asp
420 425 430
Arg Leu Met Asn Pro Leu Ile Asp Gln Tyr Leu Tyr Tyr Leu Asn Arg
435 440 445
Thr Gln Asn Gln Ser Gly Ser Ala Gln Asn Arg Asp Leu Leu Phe Ser
450 455 460
Arg Gly Ser Pro Ala Gly Met Ser Val Gln Pro Lys Asn Trp Leu Pro
465 470 475 480
Gly Pro Cys Tyr Arg Gln Gln Arg Val Ser Lys Thr Lys Thr Asp Asn
485 490 495
Asn Asn Ser Asn Phe Thr Trp Thr Gly Ala Ser Lys Tyr Asn Leu Asn
500 505 510
Gly Arg Glu Ser Ile Ile Asn Pro Gly Thr Ala Met Ala Ser His Lys
515 520 525
Asp Asp Glu Asp Lys Phe Phe Pro Met Ser Gly Val Met Ile Phe Gly
530 535 540
Lys Glu Ser Ala Gly Ala Ser Asn Thr Ala Leu Asp Asn Val Met Ile
545 550 555 560
Thr Asp Glu Glu Glu Ile Lys Ala Thr Asn Pro Val Ala Thr Glu Arg
565 570 575
Phe Gly Thr Val Ala Val Asn Phe Gln Ser Ser Ser Thr Asp Pro Ala
580 585 590
Thr Gly Asp Val His Ala Met Gly Ala Leu Pro Gly Met Val Trp Gln
595 600 605
Asp Arg Asp Val Tyr Leu Gln Gly Pro Ile Trp Ala Lys Ile Pro His
610 615 620
Thr Asp Gly His Phe His Pro Ser Pro Leu Met Gly Gly Phe Gly Leu
625 630 635 640
Lys Asn Pro Pro Pro Gln Ile Leu Ile Lys Asn Thr Pro Val Pro Ala
645 650 655
Asn Pro Pro Ala Glu Phe Ser Ala Thr Lys Phe Ala Ser Phe Ile Thr
660 665 670
Gln Tyr Ser Thr Gly Gln Val Ser Val Glu Ile Glu Trp Glu Leu Gln
675 680 685
Lys Glu Asn Ser Lys Arg Trp Asn Pro Glu Val Gln Tyr Thr Ser Asn
690 695 700
Tyr Ala Lys Ser Ala Asn Val Asp Phe Thr Val Asp Asn Asn Gly Leu
705 710 715 720
Tyr Thr Glu Pro Arg Pro Ile Gly Thr Arg Tyr Leu Thr Arg Pro Leu
725 730 735
<210>225
<211>735
<212>PRT
<213>vp1,修饰的hu.29
<400>225
Met Ala Ala Asp Gly Tyr Leu Pro Asp Trp Leu Glu Asp Thr Leu Ser
1 5 10 15
Glu Gly Ile Arg Gln Trp Trp Lys Leu Lys Pro Gly Pro Pro Pro Pro
20 25 30
Lys Pro Ala Glu Arg His Lys Asp Asp Ser Arg Gly Leu Val Leu Pro
35 40 45
Gly Tyr Lys Tyr Leu Gly Pro Phe Asn Gly Leu Asp Lys Gly Glu Pro
50 55 60
Val Asn Glu Ala Asp Ala Ala Ala Leu Glu His Asp Lys Ala Tyr Asp
65 70 75 80
Arg Gln Leu Asp Ser Gly Asp Asn Pro Tyr Leu Lys Tyr Asn His Ala
85 90 95
Asp Ala Glu Phe Gln Glu Arg Leu Lys Glu Asp Thr Ser Phe Gly Gly
100 105 110
Asn Leu Gly Arg Ala Val Phe Gln Ala Lys Lys Arg Val Leu Glu Pro
115 120 125
Leu Gly Leu Val Glu Glu Pro Val Lys Thr Ala Pro Gly Lys Lys Arg
130 135 140
Pro Val Glu His Ser Pro Ala Glu Pro Asp Ser Ser Ser Gly Thr Gly
145 150 155 160
Lys Ser Gly Asn Gln Pro Ala Arg Lys Arg Leu Asn Phe Gly Gln Thr
165 170 175
Gly Asp Ser Asp Ser Val Pro Asp Pro Gln Pro Leu Gly Gln Pro Pro
180 185 190
Ala Ala Pro Ser Gly Leu Gly Thr Asn Thr Met Ala Thr Gly Ser Gly
195 200 205
Ala Pro Met Ala Asp Asn Asn Glu Gly Ala Asp Gly Val Gly Asn Ser
210 215 220
Ser Gly Asn Trp His Cys Asp Ser Thr Trp Met Gly Asp Arg Val Ile
225 230 235 240
Thr Thr Ser Thr Arg Thr Trp Ala Leu Pro Thr Tyr Asn Asn His Leu
245 250 255
Tyr Lys Gln Ile Ser Ser Gln Ser Gly Ala Ser Asn Asp Asn His Tyr
260 265 270
Phe Gly Tyr Ser Thr Pro Trp Gly Tyr Phe Asp Phe Asn Arg Phe His
275 280 285
Cys His Phe Ser Pro Arg Asp Trp Gln Arg Leu Ile Asn Asn Asn Trp
290 295 300
Gly Phe Arg Pro Lys Arg Leu Asn Phe Lys Leu Phe Asn Ile Gln Val
305 310 315 320
Lys Glu Val Thr Gln Asn Asp Gly Thr Thr Thr Ile Ala Asn Asn Leu
325 330 335
Thr Ser Thr Val Gln Val Phe Thr Asp Ser Glu Tyr Gln Leu Pro Tyr
340 345 350
Val Leu Gly Ser Ala His Gln Gly Cys Leu Pro Pro Phe Pro Ala Asp
355 360 365
Val Phe Met Val Pro Gln Tyr Gly Tyr Leu Thr Leu Asn Asn Gly Ser
370 375 380
Gln Ala Val Gly Arg Ser Ser Phe Tyr Cys Leu Glu Tyr Phe Pro Ser
385 390 395 400
Gln Met Leu Arg Thr Gly Asn Asn Phe Thr Phe Ser Tyr Thr Phe Glu
405 410 415
Asp Val Pro Phe His Ser Ser Tyr Ala His Ser Gln Ser Leu Asp Arg
420 425 430
Leu Met Asn Pro Leu Ile Asp Gln Tyr Leu Tyr Tyr Leu Ser Arg Thr
435 440 445
Asn Thr Pro Ser Gly Thr Thr Thr Gln Ser Arg Leu Gln Phe Ser Gln
450 455 460
Ala Gly Ala Ser Asp Ile Arg Asp Gln Ser Arg Asn Trp Leu Pro Gly
465 470 475 480
Pro Cys Tyr Arg Gln Gln Arg Val Ser Lys Thr Ser Ala Asp Asn Asn
485 490 495
Asn Ser Glu Tyr Ser Trp Thr Gly Ala Thr Lys Tyr His Leu Asn Gly
500 505 510
Arg Asp Ser Leu Val Asn Pro Gly Pro Ala Met Ala Ser His Lys Asp
515 520 525
Asp Glu Glu Lys Phe Phe Pro Gln Ser Gly Val Leu Ile Phe Gly Lys
530 535 540
Gln Gly Pro Glu Lys Thr Asn Val Asp Ile Glu Lys Val Met Ile Thr
545 550 555 560
Asp Glu Glu Glu Ile Arg Thr Thr Asn Pro Val Ala Thr Glu Gln Tyr
565 570 575
Gly Ser Val Ser Thr Asn Leu Gln Ser Gly Asn Thr Gln Ala Ala Thr
580 585 590
Ala Asp Val Asn Thr Gln Gly Val Leu Pro Gly Met Val Trp Gln Asp
595 600 605
Arg Asp Val Tyr Leu Gln Gly Pro Ile Trp Ala Lys Ile Pro His Thr
610 615 620
Asp Gly His Phe His Pro Ser Pro Leu Met Gly Gly Phe Gly Leu Lys
625 630 635 640
His Pro Pro Pro Gln Ile Leu Ile Lys Asn Thr Pro Val Pro Ala Asn
645 650 655
Pro Ser Thr Thr Phe Ser Ala Ala Lys Phe Ala Ser Phe Ile Thr Gln
660 665 670
Tyr Ser Thr Gly Gln Val Ser Val Glu Ile Glu Trp Glu Leu Gln Lys
675 680 685
Glu Asn Ser Lys Arg Trp Asn Pro Glu Ile Gln Tyr Thr Ser Asn Tyr
690 695 700
Asn Lys Ser Val Asn Val Asp Phe Thr Val Asp Thr Asn Gly Val Tyr
705 710 715 720
Ser Glu Pro Arg Pro Ile Gly Thr Arg Tyr Leu Thr Arg Asn Leu
725 730 735
<210>226
<211>735
<212>PRT
<213>vpl,修饰的hu.7
<400>226
Met Ala Ala Asp Gly Tyr Leu Pro Asp Trp Leu Glu Asp Thr Leu Ser
1 5 10 15
Glu Gly Ile Arg Gln Trp Trp Lys Leu Lys Pro Gly Pro Pro Pro Pro
20 25 30
Lys Pro Ala Glu Arg His Lys Asp Asp Ser Arg Gly Leu Val Leu Pro
35 40 45
Gly Tyr Lys Tyr Leu Gly Pro Phe Asn Gly Leu Asp Lys Gly Glu Pro
50 55 60
Val Asn Glu Ala Asp Ala Ala Ala Leu Glu His Asp Lys Ala Tyr Asp
65 70 75 80
Arg Gln Leu Asp Ser Gly Asp Asn Pro Tyr Leu Lys Tyr Asn His Ala
85 90 95
Asp Ala Glu Phe Gln Glu Arg Leu Lys Glu Asp Thr Ser Phe Gly Gly
100 105 110
Asn Leu Gly Arg Ala Val Phe Gln Ala Lys Lys Arg Val Leu Glu Pro
115 120 125
Leu Gly Leu Val Glu Pro Pro Val Lys Thr Ala Pro Gly Lys Lys Arg
130 135 140
Pro Val Glu His Ser Pro Ala Glu Pro Asp Ser Ser Ser Gly Thr Gly
145 150 155 160
Lys Ala Gly Gln Gln Pro Ala Arg Lys Arg Leu Asn Phe Gly Gln Thr
165 170 175
Gly Asp Ala Asp Ser Val Pro Asp Pro Gln Pro Leu Gly Gln Pro Pro
180 185 190
Ala Ala Pro Ser Gly Leu Gly Ser Thr Thr Met Ala Thr Gly Ser Gly
195 200 205
Ala Pro Met Ala Asp Asn Asn Glu Gly Ala Asp Gly Val Gly Asn Ser
210 215 220
Ser Gly Asn Trp His Cys Asp Ser Gln Trp Leu Gly Asp Arg Val Ile
225 230 235 240
Thr Thr Ser Thr Arg Thr Trp Ala Leu Pro Thr Tyr Asn Asn His Leu
245 250 255
Tyr Lys Gln Ile Ser Ser Gln Ser Gly Ala Ser Asn Asp Asn His Tyr
260 265 270
Phe Gly Tyr Ser Thr Pro Trp Gly Tyr Phe Asp Phe Asn Arg Phe His
275 280 285
Cys His Phe Ser Pro Arg Asp Trp Gln Arg Leu Ile Asn Asn Asn Trp
290 295 300
Gly Phe Arg Pro Lys Arg Leu Asn Phe Lys Leu Phe Asn Ile Gln Val
305 310 315 320
Lys Glu Val Thr Gln Asn Asp Gly Thr Thr Thr Ile Ala Asn Asn Leu
325 330 335
Thr Ser Thr Val Gln Val Phe Thr Asp Ser Glu Tyr Gln Leu Pro Tyr
340 345 350
Val Leu Gly Ser Ala His Gln Gly Cys Leu Pro Pro Phe Pro Ala Asp
355 360 365
Val Phe Met Val Pro Gln Tyr Gly Tyr Leu Thr Leu Asn Asn Gly Ser
370 375 380
Gln Ala Val Gly Arg Ser Ser Phe Tyr Cys Leu Glu Tyr Phe Pro Ser
385 390 395 400
Gln Met Leu Arg Thr Gly Asn Asn Phe Gln Phe Ser Tyr Thr Phe Glu
405 410 415
Asp Val Pro Phe His Ser Ser Tyr Ala His Ser Gln Ser Leu Asp Arg
420 425 430
Leu Met Asn Pro Leu Ile Asp Gln Tyr Leu Tyr Tyr Leu Asn Lys Thr
435 440 445
Gln Ser Asn Ser Gly Thr Leu Gln Gln Ser Arg Leu Leu Phe Ser Gln
450 455 460
Ala Gly Pro Thr Ser Met Ser Leu Gln Ala Lys Asn Trp Leu Pro Gly
465 470 475 480
Pro Cys Tyr Arg Gln Gln Arg Leu Ser Lys Gln Ala Asn Asp Asn Asn
485 490 495
Asn Ser Asn Phe Pro Trp Thr Ala Ala Thr Lys Tyr His Leu Asn Gly
500 505 510
Arg Asp Ser Leu Val Asn Pro Gly Pro Ala Met Ala Ser His Lys Asp
515 520 525
Asp Glu Glu Lys Phe Phe Pro Met His Gly Thr Leu Ile Phe Gly Lys
530 535 540
Gln Gly Thr Asn Ala Asn Asp Ala Asp Leu Asp Asn Val Met Ile Thr
545 550 555 560
Asp Glu Glu Glu Ile Arg Thr Thr Asn Pro Val Ala Thr Glu Gln Tyr
565 570 575
Gly Tyr Val Ser Asn Asn Leu Gln Asn Ser Asn Thr Gly Pro Thr Thr
580 585 590
Gly Thr Val Asn His Gln Gly Ala Leu Pro Gly Met Val Trp Gln Asp
595 600 605
Arg Asp Val Tyr Leu Gln Gly Pro Ile Trp Ala Lys Ile Pro His Thr
610 615 620
Asp Gly His Phe His Pro Ser Pro Leu Met Gly Gly Phe Gly Leu Lys
625 630 635 640
His Pro Pro Pro Gln Ile Met Ile Lys Asn Thr Pro Val Pro Ala Asn
645 650 655
Pro Pro Thr Asn Phe Ser Ser Ala Lys Phe Ala Ser Phe Ile Thr Gln
660 665 670
Tyr Ser Thr Gly Gln Val Ser Val Glu Ile Glu Trp Glu Leu Gln Lys
675 680 685
Glu Asn Ser Lys Arg Trp Asn Pro Glu Ile Gln Tyr Thr Ser Asn Tyr
690 695 700
Asn Lys Ser Val Asn Val Asp Phe Thr Val Asp Thr Asn Gly Val Tyr
705 710 715 720
Ser Glu Pro Arg Pro Ile Gly Thr Arg Tyr Leu Thr Arg Asn Leu
725 730 735
<210>227
<211>728
<212>PRT
<213>vp1,修饰的cy.5
<400>227
Met Ala Ala Asp Gly Tyr Leu Pro Asp Trp Leu Glu Asp Asn Leu Ser
1 5 10 15
Glu Gly Ile Arg Glu Trp Trp Asp Leu Lys Pro Gly Ala Pro Lys Pro
20 25 30
Lys Ala Asn Gln Gln Lys Gln Asp Asp Gly Arg Gly Leu Val Leu Pro
35 40 45
Gly Tyr Arg Tyr Leu Gly Pro Phe Asn Gly Leu Asp Lys Gly Glu Pro
50 55 60
Val Asn Glu Ala Asp Ala Ala Ala Leu Glu His Asp Lys Ala Tyr Asp
65 70 75 80
Lys Gln Leu Glu Gln Gly Asp Asn Pro Tyr Leu Lys Tyr Asn His Ala
85 90 95
Asp Ala Glu Phe Gln Glu Arg Leu Gln Glu Asp Thr Ser Phe Gly Gly
100 105 110
Asn Leu Gly Arg Ala Val Phe Gln Ala Lys Lys Arg Val Leu Glu Pro
115 120 125
Leu Gly Leu Val Glu Glu Gly Ala Lys Thr Ala Pro Gly Lys Lys Arg
130 135 140
Pro Ile Glu Ser Pro Asp Ser Ser Thr Gly Ile Gly Lys Asn Gly Gln
145 150 155 160
Pro Pro Ala Lys Lys Lys Leu Asn Phe Gly Gln Thr Gly Asp Ser Glu
165 170 175
Ser Val Pro Asp Pro Gln Pro Leu Gly Glu Pro Pro Ala Ala Pro Ser
180 185 190
Gly Leu Gly Ser Gly Thr Met Ala Ala Gly Gly Gly Ala Pro Met Ala
195 200 205
Asp Asn Asn Glu Gly Ala Asp Gly Val Gly Asn Ala Ser Gly Asn Trp
210 215 220
His Cys Asp Ser Thr Trp Leu Gly Asp Arg Val Ile Thr Thr Ser Thr
225 230 235 240
Arg Thr Trp Ala Leu Pro Thr Tyr Asn Asn His Leu Tyr Lys Gln Ile
245 250 255
Ser Ser Gln Ser Gly Ala Thr Asn Asp Asn His Phe Phe Gly Tyr Ser
260 265 270
Thr Pro Trp Gly Tyr Phe Asp Phe Asn Arg Phe His Cys His Phe Ser
275 280 285
Pro Arg Asp Trp Gln Arg Leu Ile Asn Asn Asn Trp Gly Phe Arg Pro
290 295 300
Arg Lys Leu Arg Phe Lys Leu Phe Asn Ile Gln Val Lys Glu Val Thr
305 310 315 320
Thr Asn Asp Gly Val Thr Thr Ile Ala Asn Asn Leu Thr Ser Thr Ile
325 330 335
Gln Val Phe Ser Asp Ser Glu Tyr Gln Leu Pro Tyr Val Leu Gly Ser
340 345 350
Ala His Gln Gly Cys Leu Pro Pro Phe Pro Ala Asp Val Phe Met Ile
355 360 365
Pro Gln Tyr Gly Tyr Leu Thr Leu Asn Asn Gly Ser Gln Ser Val Gly
370 375 380
Arg Ser Ser Phe Tyr Cys Leu Glu Tyr Phe Pro Ser Gln Met Leu Arg
385 390 395 400
Thr Gly Asn Asn Phe Glu Phe Ser Tyr Thr Phe Glu Glu Val Pro Phe
405 410 415
His Ser Ser Tyr Ala His Ser Gln Ser Leu Asp Arg Leu Met Asn Pro
420 425 430
Leu Ile Asp Gln Tyr Leu Tyr Tyr Leu Ala Arg Thr Gln Ser Thr Thr
435 440 445
Gly Ser Thr Arg Glu Leu Gln Phe His Gln Ala Gly Pro Asn Thr Met
450 455 460
Ala Glu Gln Ser Lys Asn Trp Leu Pro Gly Pro Cys Tyr Arg Gln Gln
465 470 475 480
Arg Leu Ser Lys Asn Ile Asp Ser Asn Asn Asn Ser Asn Phe Ala Trp
485 490 495
Thr Gly Ala Thr Lys Tyr His Leu Asn Gly Arg Asn Ser Leu Thr Asn
500 505 510
Pro Gly Val Ala Met Ala Thr Asn Lys Asp Asp Glu Asp Gln Phe Phe
515 520 525
Pro Ile Asn Gly Val Leu Val Phe Gly Lys Thr Gly Ala Ala Asn Lys
530 535 540
Thr Thr Leu Glu Asn Val Leu Met Thr Ser Glu Glu Glu Ile Lys Thr
545 550 555 560
Thr Asn Pro Val Ala Thr Glu Glu Tyr Gly Val Val Ser Ser Asn Leu
565 570 575
Gln Ser Ser Thr Ala Gly Pro Gln Thr Gln Thr Val Asn Ser Gln Gly
580 585 590
Ala Leu Pro Gly Met Val Trp Gln Asn Arg Asp Val Tyr Leu Gln Gly
595 600 605
Pro Ile Trp Ala Lys Ile Pro His Thr Asp Gly Asn Phe His Pro Ser
610 615 620
Pro Leu Met Gly Gly Phe Gly Leu Lys His Pro Pro Pro Gln Ile Leu
625 630 635 640
Ile Lys Asn Thr Pro Val Pro Ala Asn Pro Pro Glu Val Phe Thr Pro
645 650 655
Ala Lys Phe Ala Ser Phe Ile Thr Gln Tyr Ser Thr Gly Gln Val Ser
660 665 670
Val Glu Ile Glu Trp Glu Leu Gln Lys Glu Asn Ser Lys Arg Trp Asn
675 680 685
Pro Glu Ile Gln Tyr Thr Ser Asn Tyr Ala Lys Ser Asn Asn Val Glu
690 695 700
Phe Ala Val Asn Asn Glu Gly Val Tyr Thr Glu Pro Arg Pro Ile Gly
705 710 715 720
Thr Arg Tyr Leu Thr Arg Asn Leu
725
<210>228
<211>728
<212>PRT
<213>vp1,修饰的rh.13
<400>228
Met Ala Ala Asp Gly Tyr Leu Pro Asp Trp Leu Glu Asp Asn Leu Ser
1 5 10 15
Glu Gly Ile Arg Glu Trp Trp Asp Leu Lys Pro Gly Ala Pro Lys Pro
20 25 30
Lys Ala Asn Gln Gln Lys Gln Asp Asp Gly Arg Gly Leu Val Leu Pro
35 40 45
Gly Tyr Lys Tyr Leu Gly Pro Phe Asn Gly Leu Asp Lys Gly Glu Pro
50 55 60
Val Asn Glu Ala Asp Ala Ala Ala Leu Glu His Asp Lys Ala Tyr Asp
65 70 75 80
Lys Gln Leu Glu Gln Gly Asp Asn Pro Tyr Leu Lys Tyr Asn His Ala
85 90 95
Asp Ala Glu Phe Gln Glu Arg Leu Gln Glu Asp Thr Ser Phe Gly Gly
100 105 110
Asn Leu Gly Arg Ala Val Phe Gln Ala Lys Lys Arg Val Leu Glu Pro
115 120 125
Leu Gly Leu Val Glu Glu Gly Ala Lys Thr Ala Pro Gly Lys Lys Arg
130 135 140
Pro Ile Glu Ser Pro Asp Ser Ser Thr Gly Ile Gly Lys Lys Gly Gln
145 150 155 160
Gln Pro Ala Lys Lys Lys Leu Asn Phe Gly Gln Thr Gly Asp Ser Glu
165 170 175
Ser Val Pro Asp Pro Gln Pro Leu Gly Glu Pro Pro Ala Ala Pro Ser
180 185 190
Gly Leu Gly Ser Gly Thr Met Ala Ala Gly Gly Gly Ala Pro Met Ala
195 200 205
Asp Asn Asn Glu Gly Ala Asp Gly Val Gly Asn Ala Ser Gly Asn Trp
210 215 220
His Cys Asp Ser Thr Trp Leu Gly Asp Arg Val Ile Thr Thr Ser Thr
225 230 235 240
Arg Thr Trp Ala Leu Pro Thr Tyr Asn Asn His Leu Tyr Lys Gln Ile
245 250 255
Ser Ser Gln Ser Gly Ala Thr Asn Asp Asn His Phe Phe Gly Tyr Ser
260 265 270
Thr Pro Trp Gly Tyr Phe Asp Phe Asn Arg Phe His Cys His Phe Ser
275 280 285
Pro Arg Asp Trp Gln Arg Leu Ile Asn Asn Asn Trp Gly Phe Arg Pro
290 295 300
Arg Lys Leu Arg Phe Lys Leu Phe Asn Ile Gln Val Lys Glu Val Thr
305 310 315 320
Thr Asn Asp Gly Val Thr Thr Ile Ala Asn Asn Leu Thr Ser Thr Ile
325 330 335
Gln Val Phe Ser Asp Ser Glu Tyr Gln Leu Pro Tyr Val Leu Gly Ser
340 345 350
Ala His Gln Gly Cys Leu Pro Pro Phe Pro Ala Asp Val Phe Met Ile
355 360 365
Pro Gln Tyr Gly Tyr Leu Thr Leu Asn Asn Gly Ser Gln Ser Val Gly
370 375 380
Arg Ser Ser Phe Tyr Cys Leu Glu Tyr Phe Pro Ser Gln Met Leu Arg
385 390 395 400
Thr Gly Asn Asn Phe Glu Phe Ser Tyr Thr Phe Glu Glu Val Pro Phe
405 410 415
His Ser Ser Tyr Ala His Ser Gln Ser Leu Asp Arg Leu Met Asn Pro
420 425 430
Leu Ile Asp Gln Tyr Leu Tyr Tyr Leu Ala Arg Thr Gln Ser Thr Thr
435 440 445
Gly Ser Thr Arg Glu Leu Gln Phe His Gln Ala Gly Pro Asn Thr Met
450 455 460
Ala Glu Gln Ser Lys Asn Trp Leu Pro Gly Pro Cys Tyr Arg Gln Gln
465 470 475 480
Arg Leu Ser Lys Asn Ile Asp Ser Asn Asn Asn Ser Asn Phe Ala Trp
485 490 495
Thr Gly Ala Thr Lys Tyr His Leu Asn Gly Arg Asn Ser Leu Thr Asn
500 505 510
Pro Gly Val Ala Met Ala Thr Asn Lys Asp Asp Glu Asp Gln Phe Phe
515 520 525
Pro Ile Asn Gly Val Leu Val Phe Gly Lys Thr Gly Ala Ala Asn Lys
530 535 540
Thr Thr Leu Glu Asn Val Leu Met Thr Ser Glu Glu Glu Ile Lys Thr
545 550 555 560
Thr Asn Pro Val Ala Thr Glu Glu Tyr Gly Val Val Ser Ser Asn Leu
565 570 575
Gln Ser Ser Thr Ala Gly Pro Gln Thr Gln Thr Val Asn Ser Gln Gly
580 585 590
Ala Leu Pro Gly Met Val Trp Gln Asn Arg Asp Val Tyr Leu Gln Gly
595 600 605
Pro Ile Trp Ala Lys Ile Pro His Thr Asp Gly Asn Phe His Pro Ser
610 615 620
Pro Leu Met Gly Gly Phe Gly Leu Lys His Pro Pro Pro Gln Ile Leu
625 630 635 640
Ile Lys Asn Thr Pro Val Pro Ala Asn Pro Pro Glu Val Phe Thr Pro
645 650 655
Ala Lys Phe Ala Ser Phe Ile Thr Gln Tyr Ser Thr Gly Gln Val Ser
660 665 670
Val Glu Ile Glu Trp Glu Leu Gln Lys Glu Asn Ser Lys Arg Trp Asn
675 680 685
Pro Glu Ile Gln Tyr Thr Ser Asn Tyr Ala Lys Ser Asn Asn Val Glu
690 695 700
Phe Ala Val Asn Asn Glu Gly Val Tyr Thr Glu Pro Arg Pro Ile Gly
705 710 715 720
Thr Arg Tyr Leu Thr Arg Asn Leu
725
<210>229
<211>729
<212>PRT
<213>vp1,修饰的rh.37
<400>229
Met Ala Ala Asp Gly Tyr Leu Pro Asp Trp Leu Glu Asp Asn Leu Ser
1 5 10 15
Glu Gly Ile Arg Glu Trp Trp Asp Leu Lys Pro Gly Ala Pro Lys Pro
20 25 30
Lys Ala Asn Gln Gln Lys Gln Asp Asp Gly Arg Gly Leu Val Leu Pro
35 40 45
Gly Tyr Lys Tyr Leu Gly Pro Phe Asn Gly Leu Asp Lys Gly Glu Pro
50 55 60
Val Asn Ala Ala Asp Ala Ala Ala Leu Glu His Asp Lys Ala Tyr Asp
65 70 75 80
Gln Gln Leu Lys Ala Gly Asp Asn Pro Tyr Leu Arg Tyr Asn His Ala
85 90 95
Asp Ala Glu Phe Gln Glu Arg Leu Gln Glu Asp Thr Ser Phe Gly Gly
100 105 110
Asn Leu Gly Arg Ala Val Phe Gln Ala Lys Lys Arg Val Leu Glu Pro
115 120 125
Leu Gly Leu Val Glu Glu Gly Ala Lys Thr Ala Pro Gly Lys Lys Arg
130 135 140
Pro Ile Asp Ser Pro Asp Ser Ser Thr Gly Ile Gly Lys Lys Gly Gln
145 150 155 160
Gln Pro Ala Lys Lys Lys Leu Asn Phe Gly Gln Thr Gly Asp Ser Glu
165 170 175
Ser Val Pro Asp Pro Gln Pro Leu Gly Glu Pro Pro Ala Ala Pro Ser
180 185 190
Ser Val Gly Ser Gly Thr Met Ala Ala Gly Gly Gly Ala Pro Thr Ala
195 200 205
Asp Asn Asn Glu Gly Ala Asp Gly Val Gly Asn Ala Ser Gly Asn Trp
210 215 220
His Cys Asp Ser Thr Trp Leu Gly Asp Arg Val Ile Thr Thr Ser Thr
225 230 235 240
Arg Thr Trp Ala Leu Pro Thr Tyr Asn Asn His Leu Tyr Lys Gln Ile
245 250 255
Ser Ser Ser Ser Ser Gly Ala Thr Asn Asp Asn His Tyr Phe Gly Tyr
260 265 270
Ser Thr Pro Trp Gly Tyr Phe Asp Phe Asn Arg Phe His Cys His Phe
275 280 285
Ser Pro Arg Asp Trp Gln Arg Leu Ile Asn Asn Asn Trp Gly Phe Arg
290 295 300
Pro Lys Lys Leu Arg Phe Lys Leu Phe Asn Ile Gln Val Lys Glu Val
305 310 315 320
Thr Thr Asn Asp Gly Val Thr Thr Ile Ala Asn Asn Leu Thr Ser Thr
325 330 335
Val Gln Val Phe Ser Asp Ser Glu Tyr Gln Leu Pro Tyr Val Leu Gly
340 345 350
Ser Ala His Gln Gly Cys Leu Pro Pro Phe Pro Ala Asp Val Phe Met
355 360 365
Ile Pro Gln Tyr Gly Tyr Leu Thr Leu Asn Asn Gly Ser Gln Ser Val
370 375 380
Gly Arg Ser Ser Phe Tyr Cys Leu Glu Tyr Phe Pro Ser Gln Met Leu
385 390 395 400
Arg Thr Gly Asn Asn Phe Glu Phe Ser Tyr Ser Phe Glu Asp Val Pro
405 410 415
Phe His Ser Ser Tyr Ala His Ser Gln Ser Leu Asp Arg Leu Met Asn
420 425 430
Pro Leu Ile Asp Gln Tyr Leu Tyr Tyr Leu Ala Arg Thr Gln Ser Thr
435 440 445
Thr Gly Ser Thr Arg Glu Leu Gln Phe His Gln Ala Gly Pro Asn Thr
450 455 460
Met Ala Glu Gln Ser Lys Asn Trp Leu Pro Gly Pro Cys Tyr Arg Gln
465 470 475 480
Gln Arg Leu Ser Lys Asn Leu Asp Phe Asn Asn Asn Ser Asn Phe Ala
485 490 495
Trp Thr Ala Ala Thr Lys Tyr His Leu Asn Gly Arg Asn Ser Leu Thr
500 505 510
Asn Pro Gly Ile Pro Met Ala Thr Asn Lys Asp Asp Glu Asp Gln Phe
515 520 525
Phe Pro Ile Asn Gly Val Leu Val Phe Gly Lys Thr Gly Ala Ala Asn
530 535 540
Lys Thr Thr Leu Glu Asn Val Leu Met Thr Ser Glu Glu Glu Ile Lys
545 550 555 560
Thr Thr Asn Pro Val Ala Thr Glu Glu Tyr Gly Val Val Ser Ser Asn
565 570 575
Leu Gln Ser Ser Thr Ala Gly Pro Gln Ser Gln Thr Ile Asn Ser Gln
580 585 590
Gly Ala Leu Pro Gly Met Val Trp Gln Asn Arg Asp Val Tyr Leu Gln
595 600 605
Gly Pro Ile Trp Ala Lys Ile Pro His Thr Asp Gly Asn Phe His Pro
610 615 620
Ser Pro Leu Met Gly Gly Phe Gly Leu Lys His Pro Pro Pro Gln Ile
625 630 635 640
Leu Ile Lys Asn Thr Pro Val Pro Ala Asn Pro Pro Glu Val Phe Thr
645 650 655
Pro Ala Lys Phe Ala Ser Phe Ile Thr Gln Tyr Ser Thr Gly Gln Val
660 665 670
Ser Val Glu Ile Glu Trp Glu Leu Gln Lys Glu Asn Ser Lys Arg Trp
675 680 685
Asn Pro Glu Ile Gln Tyr Thr Ser Asn Tyr Ala Lys Ser Asn Asn Val
690 695 700
Glu Phe Ala Val Asn Pro Asp Gly Val Tyr Thr Glu Pro Arg Pro Ile
705 710 715 720
Gly Thr Arg Tyr Leu Thr Arg Asn Leu
725
<210>230
<211>737
<212>PRT
<213>vp1,修饰的rh.67
<400>230
Met Ala Ala Asp Gly Tyr Leu Pro Asp Trp Leu Glu Asp Asn Leu Ser
1 5 10 15
Glu Gly Ile Arg Glu Trp Trp Asp Leu Lys Pro Gly Ala Pro Lys Pro
20 25 30
Lys Ala Asn Gln Gln Lys Gln Asp Asp Gly Arg Gly Leu Val Leu Pro
35 40 45
Gly Tyr Lys Tyr Leu Gly Pro Phe Asn Gly Leu Asp Lys Gly Glu Pro
50 55 60
Val Asn Ala Ala Asp Ala Ala Ala Leu Glu His Asp Lys Ala Tyr Asp
65 70 75 80
Gln Gln Leu Lys Ala Gly Asp Asn Pro Tyr Leu Arg Tyr Asn His Ala
85 90 95
Asp Ala Glu Phe Gln Glu Arg Leu Gln Glu Asp Thr Ser Phe Gly Gly
100 105 110
Asn Leu Gly Arg Ala Val Phe Gln Ala Lys Lys Arg Val Leu Glu Pro
115 120 125
Leu Gly Leu Val Glu Glu Ala Ala Lys Thr Ala Pro Gly Lys Lys Arg
130 135 140
Pro Val Glu Pro Ser Pro Gln Arg Ser Pro Asp Ser Ser Thr Gly Ile
145 150 155 160
Gly Lys Lys Gly Gln Gln Pro Ala Arg Lys Arg Leu Asn Phe Gly Gln
165 170 175
Thr Gly Asp Ser Glu Ser Val Pro Asp Pro Gln Pro Ile Gly Glu Pro
180 185 190
Pro Ala Gly Pro Ser Gly Leu Gly Ser Gly Thr Met Ala Ala Gly Gly
195 200 205
Gly Ala Pro Met Ala Asp Asn Asn Glu Gly Ala Asp Gly Val Gly Asn
210 215 220
Ala Ser Gly Asn Trp His Cys Asp Ser Thr Trp Leu Gly Asp Arg Val
225 230 235 240
Ile Thr Thr Ser Thr Arg Thr Trp Ala Leu Pro Thr Tyr Asn Asn His
245 250 255
Leu Tyr Lys Gln Ile Ser Ser Gln Ser Ala Gly Ser Thr Asn Asp Asn
260 265 270
Val Tyr Phe Gly Tyr Ser Thr Pro Trp Gly Tyr Phe Asp Phe Asn Arg
275 280 285
Phe His Cys His Phe Ser Pro Arg Asp Trp Gln Arg Leu Ile Asn Asn
290 295 300
Asn Trp Gly Phe Arg Pro Lys Lys Leu Asn Phe Lys Leu Phe Asn Ile
305 310 315 320
Gln Val Lys Glu Val Thr Thr Asn Asp Gly Val Thr Thr Ile Ala Asn
325 330 335
Asn Leu Thr Ser Thr Val Gln Val Phe Ser Asp Ser Glu Tyr Gln Leu
340 345 350
Pro Tyr Val Leu Gly Ser Ala His Gln Gly Cys Leu Pro Pro Phe Pro
355 360 365
Ala Asp Val Phe Met Ile Pro Gln Tyr Gly Tyr Leu Thr Leu Asn Asn
370 375 380
Gly Ser Gln Ser Val Gly Arg Ser Ser Phe Tyr Cys Leu Glu Tyr Phe
385 390 395 400
Pro Ser Gln Met Leu Arg Thr Gly Asn Asn Phe Thr Phe Ser Tyr Thr
405 410 415
Phe Glu Asp Val Pro Phe His Ser Ser Tyr Ala His Ser Gln Ser Leu
420 425 430
Asp Arg Leu Met Asn Pro Leu Ile Asp Gln Tyr Leu Tyr Tyr Leu Ala
435 440 445
Arg Thr Gln Ser Asn Ala Gly Gly Thr Ala Gly Asn Arg Glu Leu Gln
450 455 460
Phe Tyr Gln Gly Gly Pro Thr Thr Met Ala Glu Gln Ala Lys Asn Trp
465 470 475 480
Leu Pro Gly Pro Cys Phe Arg Gln Gln Arg Val Ser Lys Thr Leu Asp
485 490 495
Gln Asn Asn Asn Ser Asn Phe Ala Trp Thr Gly Ala Thr Lys Tyr His
500 505 510
Leu Asn Gly Arg Asn Ser Leu Val Asn Pro Gly Val Ala Met Ala Thr
515 520 525
His Lys Asp Asp Glu Glu Arg Phe Phe Pro Ser Ser Gly Val Leu Ile
530 535 540
Phe Gly Lys Thr Gly Ala Ala Asn Lys Thr Thr Leu Glu Asn Val Leu
545 550 555 560
Met Thr Asn Glu Glu Glu Ile Arg Pro Thr Asn Pro Val Ala Thr Glu
565 570 575
Glu Tyr Gly Thr Val Ser Ser Asn Leu Gln Ala Ala Asn Thr Ala Ala
580 585 590
Gln Thr Gln Val Val Asn Asn Gln Gly Ala Leu Pro Gly Met Val Trp
595 600 605
Gln Asn Arg Asp Val Tyr Leu Gln Gly Pro Ile Trp Ala Lys Ile Pro
610 615 620
His Thr Asp Gly Asn Phe His Pro Ser Pro Leu Met Gly Gly Phe Gly
625 630 635 640
Leu Lys His Pro Pro Pro Gln Ile Leu Ile Lys Asn Thr Pro Val Pro
645 650 655
Ala Asn Pro Pro Glu Val Phe Thr Pro Ala Lys Phe Ala Ser Phe Ile
660 665 670
Thr Gln Tyr Ser Thr Gly Gln Val Ser Val Glu Ile Glu Trp Glu Leu
675 680 685
Gln Lys Glu Asn Ser Lys Arg Trp Asn Pro Glu Ile Gln Tyr Thr Ser
690 695 700
Asn Phe Asp Lys Gln Thr Gly Val Asp Phe Ala Val Asp Ser Gln Gly
705 710 715 720
Val Tyr Ser Glu Pro Arg Pro Ile Gly Thr Arg Tyr Leu Thr Arg Asn
725 730 735
Leu
<210>231
<211>738
<212>PRT
<213>vp1,修饰的rh.2
<400>231
Met Ala Ala Asp Gly Tyr Leu Pro Asp Trp Leu Glu Asp Asn Leu Ser
1 5 10 15
Glu Gly Ile Arg Glu Trp Trp Asp Leu Lys Pro Gly Ala Pro Lys Pro
20 25 30
Lys Ala Asn Gln Gln Lys Gln Asp Asp Gly Arg Gly Leu Val Leu Pro
35 40 45
Gly Tyr Lys Tyr Leu Gly Pro Phe Asn Gly Leu Asp Lys Gly Glu Pro
50 55 60
Val Asn Ala Ala Asp Ala Ala Ala Leu Glu His Asp Lys Ala Tyr Asp
65 70 75 80
Gln Gln Leu Lys Ala Gly Asp Asn Pro Tyr Leu Arg Tyr Asn His Ala
85 90 95
Asp Ala Glu Phe Gln Glu Arg Leu Gln Glu Asp Thr Ser Phe Gly Gly
100 105 110
Asn Leu Gly Arg Ala Val Phe Gln Ala Lys Lys Arg Val Leu Glu Pro
115 120 125
Leu Gly Leu Val Glu Glu Gly Ala Lys Thr Ala Pro Gly Lys Lys Arg
130 135 140
Pro Val Glu Pro Ser Pro Gln Arg Ser Pro Asp Ser Ser Thr Gly Ile
145 150 155 160
Gly Lys Lys Gly His Gln Pro Ala Arg Lys Arg Leu Asn Phe Gly Gln
165 170 175
Thr Gly Asp Ser Glu Ser Val Pro Asp Pro Gln Pro Ile Gly Glu Pro
180 185 190
Pro Ala Gly Pro Ser Gly Leu Gly Ser Gly Thr Met Ala Ala Gly Gly
195 200 205
Gly Ala Pro Met Ala Asp Asn Asn Glu Gly Ala Asp Gly Val Gly Ser
210 215 220
Ser Ser Gly Asn Trp His Cys Asp Ser Thr Trp Leu Gly Asp Arg Val
225 230 235 240
Ile Thr Thr Ser Thr Arg Thr Trp Ala Leu Pro Thr Tyr Asn Asn His
245 250 255
Leu Tyr Lys Gln Ile Ser Asn Gly Thr Ser Gly Gly Ser Thr Asn Asp
260 265 270
Asn Thr Tyr Phe Gly Tyr Ser Thr Pro Trp Gly Tyr Phe Asp Phe Asn
275 280 285
Arg Phe His Cys His Phe Ser Pro Arg Asp Trp Gln Arg Leu Ile Asn
290 295 300
Asn Asn Trp Gly Phe Arg Pro Lys Arg Leu Asn Phe Lys Leu Phe Asn
305 310 315 320
Ile Gln Val Lys Glu Val Thr Gln Asn Glu Gly Thr Lys Thr Ile Ala
325 330 335
Asn Asn Leu Thr Ser Thr Ile Gln Val Phe Thr Asp Ser Glu Tyr Gln
340 345 350
Leu Pro Tyr Val Pro Gly Ser Ala His Gln Gly Cys Leu Pro Pro Phe
355 360 365
Pro Ala Asp Val Phe Met Ile Pro Gln Tyr Gly Tyr Leu Thr Leu Asn
370 375 380
Asn Gly Ser Gln Ala Val Gly Arg Ser Ser Phe Tyr Cys Leu Glu Tyr
385 390 395 400
Phe Pro Ser Gln Met Leu Arg Thr Gly Asn Asn Phe Glu Phe Ser Tyr
405 410 415
Thr Phe Glu Asp Val Pro Phe His Ser Ser Tyr Ala His Ser Gln Ser
420 425 430
Leu Asp Arg Leu Met Asn Pro Leu Ile Asp Gln Tyr Leu Tyr Tyr Leu
435 440 445
Ser Arg Thr Gln Ser Thr Gly Gly Thr Gln Gly Thr Gln Gln Leu Leu
450 455 460
Phe Ser Gln Ala Gly Pro Ala Asn Met Ser Ala Gln Ala Lys Asn Trp
465 470 475 480
Leu Pro Gly Pro Cys Tyr Arg Gln Gln Arg Val Ser Thr Thr Leu Ser
485 490 495
Gln Asn Asn Asn Ser Asn Phe Ala Trp Thr Gly Ala Thr Lys Tyr His
500 505 510
Leu Asn Gly Arg Asp Ser Leu Val Asn Pro Gly Val Ala Met Ala Thr
515 520 525
His Lys Asp Asp Glu Glu Arg Phe Phe Pro Ser Ser Gly Val Leu Met
530 535 540
Phe Gly Lys Gln Gly Ala Gly Lys Asp Asn Val Asp Tyr Ser Ser Val
545 550 555 560
Met Leu Thr Ser Glu Glu Glu Ile Lys Thr Thr Asn Pro Val Ala Thr
565 570 575
Glu Gln Tyr Gly Val Val Ala Asp Asn Leu Gln Gln Thr Asn Gly Ala
580 585 590
Pro Ile Val Gly Thr Val Asn Ser Gln Gly Ala Leu Pro Gly Met Val
595 600 605
Trp Gln Asn Arg Asp Val Tyr Leu Gln Gly Pro Ile Trp Ala Lys Ile
610 615 620
Pro His Thr Asp Gly Asn Phe His Pro Ser Pro Leu Met Gly Gly Phe
625 630 635 640
Gly Leu Lys His Pro Pro Pro Gln Ile Leu Ile Lys Asn Thr Pro Val
645 650 655
Pro Ala Asp Pro Pro Thr Thr Phe Ser Gln Ala Lys Leu Ala Ser Phe
660 665 670
Ile Thr Gln Tyr Ser Thr Gly Gln Val Ser Val Glu Ile Glu Trp Glu
675 680 685
Leu Gln Lys Glu Asn Ser Lys Arg Trp Asn Pro Glu Ile Gln Tyr Thr
690 695 700
Ser Asn Tyr Tyr Lys Ser Thr Asn Val Asp Phe Ala Val Asn Thr Glu
705 710 715 720
Gly Thr Tyr Ser Glu Pro Arg Pro Ile Gly Thr Arg Tyr Leu Thr Arg
725 730 735
Asn Leu
<210>232
<211>738
<212>PRT
<213>vp1,修饰的rh.58
<400>232
Met Ala Ala Asp Gly Tyr Leu Pro Asp Trp Leu Glu Asp Asn Leu Ser
1 5 10 15
Glu Gly Ile Arg Glu Trp Trp Asp Leu Lys Pro Gly Ala Pro Lys Pro
20 25 30
Lys Ala Asn Gln Gln Lys Gln Asp Asp Gly Arg Gly Leu Val Leu Pro
35 40 45
Gly Tyr Lys Tyr Leu Gly Pro Phe Asn Gly Leu Asp Lys Gly Glu Pro
50 55 60
Val Asn Ala Ala Asp Ala Ala Ala Leu Glu His Asp Lys Ala Tyr Asp
65 70 75 80
Gln Gln Leu Lys Ala Gly Asp Asn Pro Tyr Leu Arg Tyr Asn His Ala
85 90 95
Asp Ala Glu Phe Gln Glu Arg Leu Gln Glu Asp Thr Ser Phe Gly Gly
100 105 110
Asn Leu Gly Arg Ala Val Phe Gln Ala Lys Lys Arg Val Leu Glu Pro
115 120 125
Leu Gly Leu Val Glu Glu Ala Ala Lys Thr Ala Pro Gly Lys Lys Arg
130 135 140
Pro Val Glu Pro Ser Pro Gln Arg Ser Pro Asp Ser Ser Thr Gly Ile
145 150 155 160
Gly Lys Lys Gly Gln Gln Pro Ala Arg Lys Arg Leu Asn Phe Gly Gln
165 170 175
Thr Gly Asp Ser Glu Ser Val Pro Asp Pro Gln Pro Ile Gly Glu Pro
180 185 190
Pro Ala Ala Pro Ser Ser Val Gly Ser Gly Thr Met Ala Ala Gly Gly
195 200 205
Gly Ala Pro Met Ala Asp Asn Asn Glu Gly Ala Asp Gly Val Gly Ser
210 215 220
Ser Ser Gly Asn Trp His Cys Asp Ser Thr Trp Leu Gly Asp Arg Val
225 230 235 240
Ile Thr Thr Ser Thr Arg Thr Trp Ala Leu Pro Thr Tyr Asn Asn His
245 250 255
Leu Tyr Lys Gln Ile Ser Asn Gly Thr Ser Gly Gly Ser Thr Asn Asp
260 265 270
Asn Thr Tyr Phe Gly Tyr Ser Thr Pro Trp Gly Tyr Phe Asp Phe Asn
275 280 285
Arg Phe His Cys His Phe Ser Pro Arg Asp Trp Gln Arg Leu Ile Asn
290 295 300
Asn Asn Trp Gly Phe Arg Pro Lys Arg Leu Ser Phe Lys Leu Phe Asn
305 310 315 320
Ile Gln Val Lys Glu Val Thr Gln Asn Glu Gly Thr Lys Thr Ile Ala
325 330 335
Asn Asn Leu Thr Ser Thr Ile Gln Val Phe Thr Asp Ser Glu Tyr Gln
340 345 350
Leu Pro Tyr Val Leu Gly Ser Ala His Gln Gly Cys Leu Pro Pro Phe
355 360 365
Pro Ala Asp Val Phe Met Ile Pro Gln Tyr Gly Tyr Leu Thr Leu Asn
370 375 380
Asn Gly Ser Gln Ala Val Gly Arg Ser Ser Phe Tyr Cys Leu Glu Tyr
385 390 395 400
Phe Pro Ser Gln Met Leu Arg Thr Gly Asn Asn Phe Ser Phe Ser Tyr
405 410 415
Thr Phe Glu Asp Val Pro Phe His Ser Ser Tyr Ala His Ser Gln Ser
420 425 430
Leu Asp Arg Leu Met Asn Pro Leu Ile Asp Gln Tyr Leu Tyr Tyr Leu
435 440 445
Ser Arg Thr Gln Ser Thr Gly Gly Thr Ala Gly Thr Gln Gln Leu Leu
450 455 460
Phe Ser Gln Ala Gly Pro Ser Asn Met Ser Ala Gln Ala Arg Asn Trp
465 470 475 480
Leu Pro Gly Pro Cys Tyr Arg Gln Gln Arg Val Ser Thr Thr Leu Ser
485 490 495
Gln Asn Asn Asn Ser Asn Phe Ala Trp Thr Gly Ala Thr Lys Tyr His
500 505 510
Leu Asn Gly Arg Asp Ser Leu Val Asn Pro Gly Val Ala Met Ala Thr
515 520 525
Asn Lys Asp Asp Glu Asp Arg Phe Phe Pro Ser Ser Gly Ile Leu Met
530 535 540
Phe Gly Lys Gln Gly Ala Gly Lys Asp Asn Val Asp Tyr Ser Asn Val
545 550 555 560
Met Leu Thr Ser Glu Glu Glu Ile Lys Thr Thr Asn Pro Val Ala Thr
565 570 575
Glu Gln Tyr Gly Val Val Ala Asp Asn Leu Gln Gln Gln Asn Thr Ala
580 585 590
Pro Ile Val Gly Ala Val Asn Ser Gln Gly Ala Leu Pro Gly Met Val
595 600 605
Trp Gln Asn Arg Asp Val Tyr Leu Gln Gly Pro Ile Trp Ala Lys Ile
610 615 620
Pro His Thr Asp Gly Asn Phe His Pro Ser Pro Leu Met Gly Gly Phe
625 630 635 640
Gly Leu Lys His Pro Pro Pro Gln Ile Leu Ile Lys Ser Thr Pro Val
645 650 655
Pro Ala Asp Pro Pro Thr Ala Phe Asn Gln Ala Lys Leu Asn Ser Phe
660 665 670
Ile Thr Gln Tyr Ser Thr Gly Gln Val Ser Val Glu Ile Glu Trp Glu
675 680 685
Leu Gln Lys Glu Asn Ser Lys Arg Trp Asn Pro Glu Ile Gln Tyr Thr
690 695 700
Ser Asn Tyr Tyr Lys Ser Thr Asn Val Asp Phe Ala Val Asn Thr Glu
705 710 715 720
Gly Val Tyr Ser Glu Pro Arg Pro Ile Gly Thr Arg Tyr Leu Thr Arg
725 730 735
Asn Leu
<210>233
<211>738
<212>PRT
<213>vpl,修饰的rh.64
<400>233
Met Ala Ala Asp Gly Tyr Leu Pro Asp Trp Leu Glu Asp Asn Leu Ser
1 5 10 15
Glu Gly Ile Arg Glu Trp Trp Asp Leu Lys Pro Gly Ala Pro Lys Pro
20 25 30
Lys Ala Asn Gln Gln Lys Gln Asp Asp Gly Arg Gly Leu Val Leu Pro
35 40 45
Gly Tyr Lys Tyr Leu Gly Pro Phe Asn Gly Leu Asp Lys Gly Glu Pro
50 55 60
Val Asn Ala Ala Asp Ala Ala Ala Leu Glu His Asp Lys Ala Tyr Asp
65 70 75 80
Gln Gln Leu Lys Ala Gly Asp Asn Pro Tyr Leu Arg Tyr Asn His Ala
85 90 95
Asp Ala Glu Phe Gln Glu Arg Leu Gln Glu Asp Thr Ser Phe Gly Gly
100 105 110
Asn Leu Gly Arg Ala Val Phe Gln Ala Lys Lys Arg Val Leu Glu Pro
115 120 125
Leu Gly Leu Val Glu Glu Gly Ala Lys Thr Ala Pro Gly Lys Lys Arg
130 135 140
Pro Val Glu Pro Ser Pro Gln Arg Ser Pro Asp Ser Ser Thr Gly Ile
145 150 155 160
Gly Lys Lys Gly Gln Gln Pro Ala Arg Lys Arg Leu Asn Phe Gly Gln
165 170 175
Thr Gly Asp Ser Glu Ser Val Pro Asp Pro Gln Pro Ile Gly Glu Pro
180 185 190
Pro Ala Ala Pro Ser Ser Val Gly Ser Gly Thr Met Ala Ala Gly Gly
195 200 205
Gly Ala Pro Met Ala Asp Asn Asn Glu Gly Ala Asp Gly Val Gly Ser
210 215 220
Ser Ser Gly Asn Trp His Cys Asp Ser Thr Trp Leu Gly Asp Arg Val
225 230 235 240
Ile Thr Thr Ser Thr Arg Thr Trp Ala Leu Pro Thr Tyr Asn Asn His
245 250 255
Leu Tyr Lys Gln Ile Ser Asn Gly Thr Ser Gly Gly Ser Thr Asn Asp
260 265 270
Asn Thr Tyr Phe Gly Tyr Ser Thr Pro Trp Gly Tyr Phe Asp Phe Asn
275 280 285
Arg Phe His Cys His Phe Ser Pro Arg Asp Trp Gln Arg Leu Ile Asn
290 295 300
Asn Asn Trp Gly Phe Arg Pro Lys Arg Leu Ser Phe Lys Leu Phe Asn
305 310 315 320
Ile Gln Val Lys Glu Val Thr Gln Asn Glu Gly Thr Lys Thr Ile Ala
325 330 335
Asn Asn Leu Thr Ser Thr Ile Gln Val Phe Thr Asp Ser Glu Tyr Gln
340 345 350
Leu Pro Tyr Val Leu Gly Ser Ala His Gln Gly Cys Leu Pro Pro Phe
355 360 365
Pro Ala Asp Val Phe Met Ile Pro Gln Tyr Gly Tyr Leu Thr Leu Asn
370 375 380
Asn Gly Ser Gln Ala Val Gly Arg Ser Ser Phe Tyr Cys Leu Glu Tyr
385 390 395 400
Phe Pro Ser Gln Met Leu Arg Thr Gly Asn Asn Phe Ser Phe Ser Tyr
405 410 415
Thr Phe Glu Asp Val Pro Phe His Ser Ser Tyr Ala His Ser Gln Ser
420 425 430
Leu Asp Arg Leu Met Asn Pro Leu Ile Asp Gln Tyr Leu Tyr Tyr Leu
435 440 445
Ser Arg Thr Gln Ser Thr Gly Gly Thr Ala Gly Thr Gln Gln Leu Leu
450 455 460
Phe Ser Gln Ala Gly Pro Ser Asn Met Ser Ala Gln Ala Arg Asn Trp
465 470 475 480
Leu Pro Gly Pro Cys Tyr Arg Gln Gln Arg Val Ser Thr Thr Leu Ser
485 490 495
Gln Asn Asn Asn Ser Asn Phe Ala Trp Thr Gly Ala Thr Lys Tyr His
500 505 510
Leu Asn Gly Arg Asp Ser Leu Val Asn Pro Gly Val Ala Met Ala Thr
515 520 525
Asn Lys Asp Asp Glu Asp Arg Phe Phe Pro Ser Ser Gly Ile Leu Met
530 535 540
Phe Gly Lys Gln Gly Ala Gly Lys Asp Asn Val Asp Tyr Ser Asn Val
545 550 555 560
Met Leu Thr Ser Glu Glu Glu Ile Lys Thr Thr Asn Pro Val Ala Thr
565 570 575
Glu Gln Tyr Gly Val Val Ala Asp Asn Leu Gln Gln Gln Asn Thr Ala
580 585 590
Pro Ile Val Gly Ala Val Asn Ser Gln Gly Ala Leu Pro Gly Met Val
595 600 605
Trp Gln Asn Arg Asp Val Tyr Leu Gln Gly Pro Ile Trp Ala Lys Ile
610 615 620
Pro His Thr Asp Gly Asn Phe His Pro Ser Pro Leu Met Gly Gly Phe
625 630 635 640
Gly Leu Lys His Pro Pro Pro Gln Ile Leu Ile Lys Asn Thr Pro Val
645 650 655
Pro Ala Asp Pro Pro Thr Ala Phe Asn Gln Ala Lys Leu Asn Ser Phe
660 665 670
Ile Thr Gln Tyr Ser Thr Gly Gln Val Ser Val Glu Ile Glu Trp Glu
675 680 685
Leu Gln Lys Glu Asn Ser Lys Arg Trp Asn Pro Glu Ile Gln Tyr Thr
690 695 700
Ser Asn Tyr Tyr Lys Ser Thr Asn Val Asp Phe Ala Val Asn Thr Glu
705 710 715 720
Gly Val Tyr Ser Glu Pro Arg Pro Ile Gly Thr Arg Tyr Leu Thr Arg
725 730 735
Asn Leu
<210>234
<211>735
<212>PRT
<213>vp1,修饰的ch.5
<400>234
Met Ala Ala Asp Gly Tyr Leu Pro Asp Trp Leu Glu Asp Thr Leu Ser
1 5 10 15
Glu Gly Ile Arg Gln Trp Trp Lys Leu Lys Pro Gly Pro Pro Pro Pro
20 25 30
Lys Pro Asn Gln Gln His Arg Asp Asp Ser Arg Gly Leu Val Leu Pro
35 40 45
Gly Tyr Lys Tyr Leu Gly Pro Phe Asn Gly Leu Asp Lys Gly Glu Pro
50 55 60
Val Asn Glu Ala Asp Ala Ala Ala Leu Glu His Asp Lys Ala Tyr Asp
65 70 75 80
His Gln Leu Lys Gln Gly Asp Asn Pro Tyr Leu Lys Tyr Asn His Ala
85 90 95
Asp Ala Glu Phe Gln Glu Arg Leu Gln Glu Asp Thr Ser Phe Gly Gly
100 105 110
Asn Leu Gly Arg Ala Val Phe Gln Ala Lys Lys Arg Val Leu Glu Pro
115 120 125
Leu Gly Leu Val Glu Glu Ala Val Lys Thr Ala Pro Gly Lys Lys Arg
130 135 140
Pro Ile Glu Gln Ser Pro Ala Glu Pro Asp Ser Ser Ser Gly Ile Gly
145 150 155 160
Lys Ser Gly Gln Gln Pro Ala Lys Lys Arg Leu Asn Phe Gly Gln Thr
165 170 175
Gly Asp Thr Glu Ser Val Pro Asp Pro Gln Pro Ile Gly Glu Pro Pro
180 185 190
Ala Ala Pro Ser Gly Val Gly Ser Asn Thr Met Ala Ser Gly Gly Gly
195 200 205
Ala Pro Met Ala Asp Asn Asn Glu Gly Ala Asp Gly Val Gly Asn Ser
210 215 220
Ser Gly Asn Trp His Cys Asp Ser Thr Trp Met Gly Asp Arg Val Ile
225 230 235 240
Thr Thr Ser Thr Arg Thr Trp Ala Leu Pro Thr Tyr Asn Asn His Leu
245 250 255
Tyr Lys Gln Ile Ser Ser Glu Ser Gly Ala Thr Asn Asp Asn His Tyr
260 265 270
Phe Gly Tyr Ser Thr Pro Trp Gly Tyr Phe Asp Phe Asn Arg Phe His
275 280 285
Cys His Phe Ser Pro Arg Asp Trp Gln Arg Leu Ile Asn Asn Asn Trp
290 295 300
Gly Phe Arg Pro Lys Lys Leu Asn Phe Lys Leu Phe Asn Ile Gln Val
305 310 315 320
Lys Glu Val Thr Gln Asn Asp Gly Thr Thr Thr Ile Ala Asn Asn Leu
325 330 335
Thr Ser Thr Val Gln Val Phe Thr Asp Ser Glu Tyr Gln Leu Pro Tyr
340 345 350
Val Leu Gly Ser Ala His Gln Gly Cys Leu Pro Pro Phe Pro Ala Asp
355 360 365
Val Phe Met Ile Pro Gln Tyr Gly Tyr Leu Thr Leu Asn Asn Gly Ser
370 375 380
Gln Ala Val Gly Arg Ser Ser Phe Tyr Cys Leu Glu Tyr Phe Pro Ser
385 390 395 400
Gln Met Leu Arg Thr Gly Asn Asn Phe Thr Phe Ser Tyr Thr Phe Glu
405 410 415
Asp Val Pro Phe His Ser Ser Tyr Ala His Ser Gln Ser Leu Asp Arg
420 425 430
Leu Met Asn Pro Leu Ile Asp Gln Tyr Leu Tyr Tyr Leu Ser Lys Thr
435 440 445
Gln Gly Thr Ser Gly Thr Thr Gln Gln Ser Arg Leu Gln Phe Ser Gln
450 455 460
Ala Gly Pro Ser Ser Met Ala Gln Gln Ala Lys Asn Trp Leu Pro Gly
465 470 475 480
Pro Ser Tyr Arg Gln Gln Arg Met Ser Lys Thr Ala Asn Asp Asn Asn
485 490 495
Asn Ser Glu Phe Ala Trp Thr Ala Ala Thr Lys Tyr Tyr Leu Asn Gly
500 505 510
Arg Asn Ser Leu Val Asn Pro Gly Pro Pro Met Ala Ser His Lys Asp
515 520 525
Asp Glu Glu Lys Tyr Phe Pro Met His Gly Asn Leu Ile Phe Gly Lys
530 535 540
Gln Gly Thr Gly Thr Thr Asn Val Asp Ile Glu Ser Val Leu Ile Thr
545 550 555 560
Asp Glu Glu Glu Ile Arg Thr Thr Asn Pro Val Ala Thr Glu Gln Tyr
565 570 575
Gly Gln Val Ala Thr Asn His Gln Ser Gln Asn Thr Thr Ala Ser Tyr
580 585 590
Gly Ser Val Asp Ser Gln Gly Ile Leu Pro Gly Met Val Trp Gln Asp
595 600 605
Arg Asp Val Tyr Leu Gln Gly Pro Ile Trp Ala Lys Ile Pro His Thr
610 615 620
Asp Gly His Phe His Pro Ser Pro Leu Met Gly Gly Phe Gly Leu Lys
625 630 635 640
His Pro Pro Pro Gln Ile Leu Ile Lys Asn Thr Pro Val Pro Ala Asn
645 650 655
Pro Ala Thr Thr Phe Thr Pro Gly Lys Phe Ala Ser Phe Ile Thr Gln
660 665 670
Tyr Ser Thr Gly Gln Val Ser Val Glu Ile Glu Trp Glu Leu Gln Lys
675 680 685
Glu Asn Ser Lys Arg Trp Asn Pro Glu Ile Gln Tyr Thr Ser Asn Tyr
690 695 700
Asn Lys Ser Val Asn Val Glu Phe Thr Val Asp Ala Asn Gly Val Tyr
705 710 715 720
Ser Glu Pro Arg Pro Ile Gly Thr Arg Tyr Leu Thr Arg Asn Leu
725 730 735
<210>235
<21l>736
<212>PRT
<213>vp1,修饰的rh.8
<400>235
Met Ala Ala Asp Gly Tyr Leu Pro Asp Trp Leu Glu Asp Asn Leu Ser
1 5 10 15
Glu Gly Ile Arg Glu Trp Trp Asp Leu Lys Pro Gly Ala Pro Lys Pro
20 25 30
Lys Ala Asn Gln Gln Lys Gln Asp Asp Gly Arg Gly Leu Val Leu Pro
35 40 45
Gly Tyr Lys Tyr Leu Gly Pro Phe Asn Gly Leu Asp Lys Gly Glu Pro
50 55 60
Val Asn Ala Ala Asp Ala Ala Ala Leu Glu His Asp Lys Ala Tyr Asp
65 70 75 80
Gln Gln Leu Lys Ala Gly Asp Asn Pro Tyr Leu Arg Tyr Asn His Ala
85 90 95
Asp Ala Glu Phe Gln Glu Arg Leu Gln Glu Asp Thr Ser Phe Gly Gly
100 105 110
Asn Leu Gly Arg Ala Val Phe Gln Ala Lys Lys Arg Val Leu Glu Pro
115 120 125
Leu Gly Leu Val Glu Glu Gly Ala Lys Thr Ala Pro Gly Lys Lys Arg
130 135 140
Pro Val Glu Gln Ser Pro Gln Glu Pro Asp Ser Ser Ser Gly Ile Gly
145 150 155 160
Lys Thr Gly Gln Gln Pro Ala Lys Lys Arg Leu Asn Phe Gly Gln Thr
165 170 175
Gly Asp Ser Glu Ser Val Pro Asp Pro Gln Pro Leu Gly Glu Pro Pro
180 185 190
Ala Ala Pro Ser Gly Leu Gly Pro Asn Thr Met Ala Ser Gly Gly Gly
195 200 205
Ala Pro Met Ala Asp Asn Asn Glu Gly Ala Asp Gly Val Gly Asn Ser
210 215 220
Ser Gly Asn Trp His Cys Asp Ser Thr Trp Leu Gly Asp Arg Val Ile
225 230 235 240
Thr Thr Ser Thr Arg Thr Trp Ala Leu Pro Thr Tyr Asn Asn His Leu
245 250 255
Tyr Lys Gln Ile Ser Asn Gly Thr Ser Gly Gly Ser Thr Asn Asp Asn
260 265 270
Thr Tyr Phe Gly Tyr Ser Thr Pro Trp Gly Tyr Phe Asp Phe Asn Arg
275 280 285
Phe His Cys His Phe Ser Pro Arg Asp Trp Gln Arg Leu Ile Asn Asn
290 295 300
Asn Trp Gly Phe Arg Pro Lys Arg Leu Asn Phe Lys Leu Phe Asn Ile
305 310 315 320
Gln Val Lys Glu Val Thr Thr Asn Glu Gly Thr Lys Thr Ile Ala Asn
325 330 335
Asn Leu Thr Ser Thr Val Gln Val Phe Thr Asp Ser Glu Tyr Gln Leu
340 345 350
Pro Tyr Val Leu Gly Ser Ala His Gln Gly Cys Leu Pro Pro Phe Pro
355 360 365
Ala Asp Val Phe Met Val Pro Gln Tyr Gly Tyr Leu Thr Leu Asn Asn
370 375 380
Gly Ser Gln Ala Leu Gly Arg Ser Ser Phe Tyr Cys Leu Glu Tyr Phe
385 390 395 400
Pro Ser Gln Met Leu Arg Thr Gly Asn Asn Phe Gln Phe Ser Tyr Thr
405 410 415
Phe Glu Asp Val Pro Phe His Ser Ser Tyr Ala His Ser Gln Ser Leu
420 425 430
Asp Arg Leu Met Asn Pro Leu Ile Asp Gln Tyr Leu Tyr Tyr Leu Val
435 440 445
Arg Thr Gln Thr Thr Gly Thr Gly Gly Thr Gln Thr Leu Ala Phe Ser
450 455 460
Gln Ala Gly Pro Ser Ser Met Ala Asn Gln Ala Arg Asn Trp Val Pro
465 470 475 480
Gly Pro Cys Tyr Arg Gln Gln Arg Val Ser Thr Thr Thr Asn Gln Asn
485 490 495
Asn Asn Ser Asn Phe Ala Trp Thr Gly Ala Ala Lys Phe Lys Leu Asn
500 505 510
Gly Arg Asp Ser Leu Met Asn Pro Gly Val Ala Met Ala Ser His Lys
515 520 525
Asp Asp Glu Asp Arg Phe Phe Pro Ser Ser Gly Val Leu Ile Phe Gly
530 535 540
Lys Gln Gly Ala Gly Asn Asp Gly Val Asp Tyr Ser Gln Val Leu Ile
545 550 555 560
Thr Asp Glu Glu Glu Ile Lys Ala Thr Asn Pro Val Ala Thr Glu Glu
565 570 575
Tyr Gly Ala Val Ala Ile Asn Asn Gln Ala Ala Asn Thr Gln Ala Gln
580 585 590
Thr Gly Leu Val His Asn Gln Gly Val Ile Pro Gly Met Val Trp Gln
595 600 605
Asn Arg Asp Val Tyr Leu Gln Gly Pro Ile Trp Ala Lys Ile Pro His
610 615 620
Thr Asp Gly Asn Phe His Pro Ser Pro Leu Met Gly Gly Phe Gly Leu
625 630 635 640
Lys His Pro Pro Pro Gln Ile Leu Ile Lys Asn Thr Pro Val Pro Ala
645 650 655
Asp Pro Pro Leu Thr Phe Asn Gln Ala Lys Leu Asn Ser Phe Ile Thr
660 665 670
Gln Tyr Ser Thr Gly Gln Val Ser Val Glu Ile Glu Trp Glu Leu Gln
675 680 685
Lys Glu Asn Ser Lys Arg Trp Asn Pro Glu Ile Gln Tyr Thr Ser Asn
690 695 700
Tyr Tyr Lys Ser Thr Asn Val Asp Phe Ala Val Asn Thr Glu Gly Val
705 710 715 720
Tyr Ser Glu Pro Arg Pro Ile Gly Thr Arg Tyr Leu Thr Arg Asn Leu
725 730 735
<210>236
<211>737
<212>PRT
<213>vpl,修饰的hu.43
<400>236
Met Ala Ala Asp Gly Tyr Leu Pro Asp Trp Leu Glu Asp Asn Leu Ser
1 5 10 15
Glu Gly Ile Arg Glu Trp Trp Asp Leu Lys Pro Gly Ala Pro Lys Pro
20 25 30
Lys Ala Asn Gln Gln Lys Gln Asp Asp Gly Arg Gly Leu Val Leu Pro
35 40 45
Gly Tyr Lys Tyr Leu Gly Pro Phe Asn Gly Leu Asp Lys Gly Glu Pro
50 55 60
Val Asn Ala Ala Asp Ala Ala Ala Leu Glu His Asp Lys Ala Tyr Asp
65 70 75 80
Gln Gln Leu Lys Ala Gly Asp Asn Pro Tyr Pro Arg Tyr Asn His Ala
85 90 95
Asp Ala Glu Phe Gln Glu Arg Leu Gln Glu Asp Thr Ser Phe Gly Gly
100 105 110
Asn Leu Gly Arg Ala Val Phe Gln Ala Lys Lys Arg Val Leu Glu Pro
115 120 125
Leu Gly Leu Val Glu Glu Ala Ala Lys Thr Ala Pro Gly Lys Lys Arg
130 135 140
Pro Val Glu Pro Ser Pro Gln Arg Ser Pro Asp Ser Ser Thr Gly Ile
145 150 155 160
Gly Lys Lys Gly Gln Gln Pro Ala Lys Lys Arg Leu Asn Phe Gly Gln
165 170 175
Thr Gly Asp Ser Glu Ser Val Pro Asp Pro Gln Pro Ile Gly Glu Pro
180 185 190
Pro Ala Gly Pro Ser Gly Leu Gly Ser Gly Thr Met Ala Ala Gly Gly
195 200 205
Gly Ala Pro Met Ala Asp Asn Asn Glu Gly Ala Asp Gly Val Gly Asn
210 215 220
Ala Ser Gly Asn Trp His Cys Asp Ser Thr Trp Leu Gly Asp Arg Val
225 230 235 240
Ile Thr Thr Ser Thr Arg Thr Trp Ala Leu Pro Thr Tyr Asn Asn His
245 250 255
Leu Tyr Lys Gln Ile Ser Ser Ala Ser Thr Gly Ala Ser Asn Asp Asn
260 265 270
His Tyr Phe Gly Tyr Ser Thr Pro Trp Gly Tyr Phe Asp Phe Asn Arg
275 280 285
Phe His Cys His Phe Ser Pro Arg Asp Trp Gln Arg Leu Ile Asn Asn
290 295 300
Asn Trp Gly Phe Arg Pro Lys Arg Leu Asn Phe Lys Leu Phe Asn Ile
305 310 315 320
Gln Val Lys Glu Val Thr Thr Asn Asp Gly Val Thr Thr Ile Ala Asn
325 330 335
Asn Leu Thr Ser Thr Val Gln Val Phe Ser Asp Ser Glu Tyr Gln Leu
340 345 350
Pro Tyr Val Leu Gly Ser Ala His Gln Gly Cys Leu Pro Pro Phe Pro
355 360 365
Ala Asp Val Phe Met Ile Pro Gln Tyr Gly Tyr Leu Thr Leu Asn Asn
370 375 380
Gly Ser Gln Ala Val Gly Arg Ser Ser Phe Tyr Cys Leu Glu Tyr Phe
385 390 395 400
Pro Ser Gln Met Leu Arg Thr Gly Asn Asn Phe Thr Phe Ser Tyr Thr
405 410 415
Phe Glu Glu Val Pro Leu His Ser Ser Tyr Ala His Ser Gln Ser Leu
420 425 430
Asp Arg Leu Met Asn Pro Leu Ile Asp Gln Tyr Leu Tyr Tyr Leu Asn
435 440 445
Arg Thr Gln Asn Gln Ser Gly Ser Ala Gln Asn Lys Asp Leu Leu Phe
450 455 460
Ser Arg Gly Ser Pro Ala Gly Met Ser Val Gln Pro Lys Asn Trp Leu
465 470 475 480
Pro Gly Pro Cys Tyr Arg Gln Gln Arg Val Ser Lys Thr Lys Thr Asp
485 490 495
Asn Asn Asn Ser Asn Phe Thr Trp Thr Gly Ala Ser Lys Tyr Asn Leu
500 505 510
Asn Gly Arg Glu Ser Ile Ile Asn Pro Gly Thr Ala Met Ala Ser His
515 520 525
Lys Asp Asp Glu Asp Lys Phe Phe Pro Met Ser Gly Val Met Ile Phe
530 535 540
Gly Lys Glu Ser Ala Gly Ala Ser Asn Thr Ala Leu Asp Asn Val Met
545 550 555 560
Ile Thr Asp Glu Glu Glu Ile Lys Ala Thr Asn Pro Val Ala Thr Glu
565 570 575
Arg Phe Gly Thr Val Ala Val Asn Phe Gln Ser Ser Ser Thr Asp Pro
580 585 590
Ala Thr Gly Asp Val His Ala Met Gly Ala Leu Pro Gly Met Val Trp
595 600 605
Gln Asp Arg Asp Val Tyr Leu Gln Gly Pro Ile Trp Ala Lys Ile Pro
610 615 620
His Thr Asp Gly His Phe His Pro Ser Pro Leu Met Gly Gly Phe Gly
625 630 635 640
Leu Lys Asn Pro Pro Pro Gln Ile Leu Ile Lys Asn Thr Pro Val Pro
645 650 655
Ala Asn Pro Pro Ala Glu Phe Ser Ala Thr Lys Phe Ala Ser Phe Ile
660 665 670
Thr Gln Tyr Ser Thr Gly Gln Val Ser Val Glu Ile Glu Trp Glu Leu
675 680 685
Gln Lys Glu Asn Ser Lys Arg Trp Asn Pro Glu Val Gln Tyr Thr Ser
690 695 700
Asn Tyr Ala Lys Ser Ala Ser Val Asp Phe Thr Val Asp Asn Asn Gly
705 710 715 720
Leu Tyr Thr Glu Pro Arg Pro Ile Gly Thr Arg Tyr Leu Thr Arg Pro
725 730 735
Leu
Claims (58)
1.一种包括至少3个AAV成员的腺伴随病毒(AAV)进化支,其中所述AAV进化支的每个成员用自引值至少为75%每1000个分离物的邻接启发式算法与不大于0.05的泊松校正距离测量值确定为系统发生相关。
2.如权利要求1所述的AAV进化支,其特征在于,所述进化支选自以下进化支:包括成员AAV8的进化支E、包括成员AAV7的进化支D、包括成员AAV9的进化支F、包括成员AAV2的进化支B与包括成员H2的进化支C。
3.如权利要求1所述的AAV进化支,其特征在于,一个或多个所述AAV成员是非天然AAV。
4.如权利要求1所述的AAV进化支,其特征在于,一个或多个所述AAV成员是天然AAV。
5.一种包括AAV8与至少2个其它AAV成员的腺伴随病毒(AAV)进化支E,其中所述AAV进化支的每个成员用自引值至少为75%每1000个分离物的邻接启发式算法与不大于0.05的泊松校正距离测量值确定为系统发生相关。
6.如权利要求5所述的AAV进化支E,其特征在于,一个或多个所述AAV成员具有与AAV8,SEQ ID NO:183的衣壳至少具有85%氨基酸同一性的衣壳。
7.如权利要求5所述的AAV进化支E,其特征在于,所述进化支至少包括:AAV8 SEQ ID NO:183、43.1/rh.2、rh.4、rh.8、44.2/rh.10、rh.25、29.3/bb.1、29.5/bb.2、30.10/pi.1(SEQ ID NO:28和93)、30.12/pi.2(SEQ ID NO:30和95)、30.19/pi.3(SEQ ID NO:29和94)、LG-4/rh.38(SEQ ID NO:7和86)、LG-10/rh.40(SEQ ID NO:14和92)、N721-8/rh.43(SEQ ID NO:43和163)、1-8/rh.49(SEQ IDNO:25和103)、2-4/rh.50(SEQ ID NO:23和108)、2-5/rh.51(SEQ ID NO:22和104)、3-9/rh.52(SEQ ID NO:18和96)、3-11/rh.53(SEQ ID NO:17和97)、5-3/rh.57(SEQID NO:26和105)、5-22/rh.58(SEQ ID NO:27和58)、修饰的rh.58(SEQ IDNO:232)、2-3/rh.61(SEQ ID NO:21和107)、4-8/rh.64(SEQ ID NO:15和99)、修饰的rh.64(SEQ ID NO:233)、3.1/hu.6(SEQ ID NO:5和84)、33.12/hu.17(SEQ IDNO:4和83)、106.1/hu.37(SEQ ID NO:10和88)、LG-9/hu.39(SEQ ID NO:24和102)、114.3/hu.40(SEQ ID NO:11和87)、127.2/hu.41(SEQ ID NO:6和91)、127.5/hu.42(SEQ ID NO:8和85)、hu.66(SEQ ID NO:173和197)、修饰的rh.2(SEQID NO:231)和hu.67(SEQ ID NO:174和198)。
8.如权利要求5所述进化支E的一种AAV,前提是所述进化支的AAV均不具有AAV8、43.1/rh.2、rh.4、rh.8、44.2/rh.10、rh.25、29.3/bb.1或29.5/bb.2的衣壳。
9.如权利要求8所述的AAV,其特征在于,所述AAV选自:30.10/pi.1(SEQ IDNO:28和93)、30.12/pi.2(SEQ ID NO:30和95)、30.19/pi.3(SEQ ID NO:29和94)、LG-4/rh.38(SEQ ID NO:7和86)、LG-10/rh.40(SEQ ID NO:14和92)、N721-8/rh.43(SEQ ID NO:43和163)、1-8/rh.49(SEQ ID NO:25和103)、2-4/rh.50(SEQ IDNO:23和108)、2-5/rh.51(SEQ ID NO:22和104)、3-9/rh.52(SEQ ID NO:18和96)、3-11/rh.53(SEQ ID NO:17和97)、5-3/rh.57(SEQ ID NO:26和105)、5-22/rh.58(SEQ ID NO:27和58)、修饰的rh.58(SEQ ID NO:232)、2-3/rh.61(SEQ ID NO:21和107)、4-8/rh.64(SEQ ID NO:15和99)、修饰的rh.64(SEQ ID NO:233)、3.1/hu.6(SEQ ID NO:5和84)、33.12/hu.17(SEQ ID NO:4和83)、106.1/hu.37(SEQ IDNO:10和88)、LG-9/hu.39(SEQ ID NO:24和102)、114.3/hu.40(SEQ ID NO:11和87)、127.2/hu.41(SEQ ID NO:6和91)、127.5/hu.42(SEQ ID NO:8和85)、hu.66(SEQ ID NO:173和197)、修饰的rh.2(SEQ ID NO:231)和hu.67(SEQ ID NO:174和198)。
10.一种包括AAV7与至少2个其它AAV成员的腺伴随病毒(AAV)进化支D,其中所述AAV进化支的每个成员用自引值至少为75%每1000个分离物的邻接启发式算法与不大于0.05的泊松校正距离测量值确定为系统发生相关。
11.如权利要求10所述的AAV进化支D,其特征在于,所述进化支至少包括以下AAV来源:AAV7、cy.2、cy.3、cy.4、cy.5、cy.6、rh.13、rh.37、rh.36、rh.35、2-15/rh.62(SEQ ID NO:33和114)、1-7/rh.48(SEQ ID NO:32和115)、4-9/rh.54(SEQ ID NO:40和116)、4-19/rh.55(SEQ ID NO:37和117)修饰的cy.5(SEQ ID NO:227)、修饰的rh.13(SEQ ID NO:228)和修饰的rh.37(SEQ IDNO:229)。
12.如权利要求10所述AAV进化支D的一种AAV,前提是所述进化支的AAV均不具有AAV7 SEQ ID NO:180和181、AAV7、cy.2、cy.3、cy.4、cy.5、cy.6、rh.13、rh.37、rh.36、rh.35的衣壳。
13.如权利要求12所述的AAV,其特征在于,所述AAV选自:2-15/rh.62(SEQID NO:33和114)、1-7/rh.48(SEQ ID NO:32和115)、4-9/rh.54(SEQ ID NO:40和116)、4-19/rh.55(SEQ ID NO:37和117)、修饰的cy.5(SEQ ID NO:227)、修饰的rh.13(SEQ ID NO:228)和修饰的rh.37(SEQ ID NO:229)。
14.一种包括AAV2与至少2个其它AAV成员的腺伴随病毒(AAV)进化支B,其中所述AAV进化支的每个成员用自引值至少为75%每1000个分离物的邻接启发式算法与不大于0.05的泊松校正距离测量值确定为系统发生相关。
15.如权利要求14所述的AAV进化支B,其特征在于,所述进化支至少包括以下AAV:AAV2、52/hu.19(SEQ ID NO:62和133)、52.1/hu.20(SEQ ID NO:63和134)、54.5/hu.23(SEQ ID NO:60和137)、54.2/hu.22(SEQ ID NO:67和138)、54.7/hu.24(SEQ ID NO:66和136)、54.1/hu.21(SEQ ID NO:65和135)、54.4R/hu.27(SEQ ID NO:64和140)、46.2/hu.28(SEQ ID NO:68和130)、46.6/hu.29(SEQ IDNO:69和132)、修饰的hu.29(SEQ ID NO:225)、172.1/hu.63(SEQ ID NO:171和195)、172.2/hu.64(SEQ ID NO:172和196)、24.5/hu.13(SEQ ID NO:71和129)、145.6/hu.56(SEQ ID NO:168和192)、hu.57(SEQ ID NO:169和193)、136.1/hu.49(SEQ ID NO:165和189)、156.1/hu.58(SEQ ID NO:179和194)、72.2/hu.34(SEQID NO:72和125)、72.3/hu.35(SEQ ID NO:73和164)、129.1/hu.45(SEQ ID NO:76和127)、130.1/hu.47(SEQ ID NO:77和128)、140.1/hu.51(SEQ ID NO:161和190)和140.2/hu.52(SEQ ID NO:167和191)。
16.如权利要求14所述AAV进化支B的一种成员AAV,前提是所述进化支的AAV均不具有AAV2的衣壳。
17.如权利要求16所述的成员AAV,其特征在于,所述AAV选自:52/hu.19(SEQ ID NO:62和133)、52.1/hu.20(SEQ ID NO:63和134)、54.5/hu.23(SEQ ID NO:60和137)、54.2/hu.22(SEQ ID NO:67和138)、54.7/hu.24(SEQ IDNO:66和136)、54.1/hu.21(SEQ ID NO:65和135)、54.4R/hu.27(SEQ ID NO:64和140)、46.2/hu.28(SEQ ID NO:68和130)、46.6/hu.29(SEQ ID NO:69和132)、修饰的hu.29(SEQ ID NO:225)、172.1/hu.63(SEQ ID NO:171和195)、172.2/hu.64(SEQ ID NO:172和196)、24.5/hu.13(SEQ ID NO:71和129)、145.6/hu.56(SEQ IDNO:168和192)、hu.57(SEQ ID NO:169和193)、136.1/hu.49(SEQ ID NO:165和189)、156.1/hu.58(SEQ ID NO:179和194)、72.2/hu.34(SEQ ID NO:72和125)、72.3/hu.35(SEQ ID NO:73和164)、129.1/hu.45(SEQ ID NO:76和127)、130.1/hu.47(SEQ ID NO:77和128)、140.1/hu.51(SEQ ID NO:161和190)和140.2/hu.52(SEQ ID NO:167和191)。
18.一种包括AAV1、AAV6与至少1个其它AAV成员的腺伴随病毒(AAV)进化支A,其中所述AAV进化支的每个成员用自引值至少为75%每1000个分离物的邻接启发式算法与不大于0.05的泊松校正距离测量值确定为系统发生相关。
19.如权利要求18所述的AAV进化支A,其特征在于,所述进化支至少包括以下AAV:AAV1、AAV6、128.1/hu.43、修饰的hu.43(SEQ ID NO:236)、128.3/hu.44(SEQ ID NO:81和158)、hu.46(SEQ ID NO:82和159)、修饰的hu.46(SEQ ID NO:224)和130.4/hu.48(SEQ ID NO:78和157)。
20.如权利要求19所述AAV进化支A的一种成员AAV,前提是所述进化支的AAV均不具有AAV1或AAV6的衣壳。
21.如权利要求19所述的成员AAV,其特征在于,所述成员AAV选自:128.1/hu.43、修饰的hu.43(SEQ ID NO:236)、128.3/hu.44(SEQ ID NO:81和158)、hu.46(SEQ ID NO:82和159)、修饰的hu.46(SEQ ID NO:224)和130.4/hu.48(SEQID NO:78和157)。
22.一种包括A3.1/ch.5、H-6/hu.4、H-2/hu.2与至少1个其它成员AAV的腺伴随病毒(AAV)进化支C,其中所述AAV进化支的每个成员用自引值至少为75%每1000个分离物的邻接启发式算法与不大于0.05的泊松校正距离测量值确定为系统发生相关。
23.如权利要求21所述的AAV进化支C,其特征在于,所述进化支至少包括以下AAV:H-6/hu.4、H-2/hu.2、3.1/hu.9(SEQ ID NO:58和155)、16.8/hu.10(SEQID NO:56和156)、16.12/hu.11(SEQ ID NO:57和153)、145.1/hu.53(SEQ IDNO:176和186)、145.6/hu.55(SEQ ID NO:178和187)、145.5/hu.54(SEQ IDNO:177和188)、7.3/hu.7(SEQ ID NO:55和150)、修饰的hu.7(SEQ ID NO:226)、33.4/hu.15(SEQ ID NO:50和147)、33.8/hu.16(SEQ ID NO:51和148)、58.2/hu.25(SEQ ID NO:49和146)、161.10/hu.60(SEQ ID NO:170和184)、H-5/hu.3(SEQ IDNO:44和145)、hu.18(SEQ ID NO:52和149)、H-1/hu.1(SEQ ID NO:46和144)和161.6/hu.61(SEQ ID NO:174和185)。
24.如权利要求21所述进化支C的成员AAV,前提是所述进化支的AAV均不具有A3.1/ch.5、H-6/hu.4或H-2/hu.2的衣壳。
25.如权利要求24所述的成员AAV,其特征在于,所述AAV选自:3.1/hu.9(SEQ ID NO:58和155)、16.8/hu.10(SEQ ID NO:56和156)、16.12/hu.11(SEQ IDNO:57和153)、145.1/hu.53(SEQ ID NO:176和186)、145.6/hu.55(SEQ ID NO:178和187)、145.5/hu.54(SEQ ID NO:177和188)、7.3/hu.7(SEQ ID NO:55和150)、修饰的hu.7(SEQ ID NO:226)、33.4/hu.15(SEQ ID NO:50和147)、33.8/hu.16(SEQID NO:51和148)、58.2/hu.25(SEQ ID NO:49和146)、161.10/hu.60(SEQ IDNO:170和184)、T1-5/hu.3(SEQ ID NO:44和145)、H-1/hu.1(SEQ ID NO:46和144)、hu.18(SEQ ID NO:52和149)和161.6/hu.61(SEQ ID NO:174和185)。
26.一种包括AAV9与至少2个其它成员AAV的腺伴随病毒(AAV)进化支F,其中所述AAV进化支的每个成员用自引值至少为75%每1000个分离物的邻接启发式算法与不大于0.05的泊松校正距离测量值确定为系统发生相关。
27.如权利要求26所述的AAV进化支F,其特征在于,所述进化支至少包括以下AAV:hu.14/AAV9(SEQ ID NO:3和123)、hu.31(SEQ ID NO:1和121)与hu.32(SEQ ID NO:2和122)。
28.一种如权利要求26所述AAV进化支F的成员AAV。
29.如权利要求28所述的成员AAV,其特征在于,所述AAV选自:hu.14/AAV9(SEQ ID NO:3和123)、hu.31(SEQ ID NO:1和121)与hu.32(SEQ IDNO:2和122)。
30一种血清型9的腺伴随病毒(AAV),所述AAV包含AAV衣壳和用于递送至细胞的异源分子,其中所述AAV衣壳在血清学上与SEQ ID NO:123所示氨基酸1-736序列的衣壳相关并且在血清学上不同于AAV1、AAV2、AAV3、AAV4、AAV5、AAV6、AAV7或AAV8中任一种的衣壳蛋白。
31.如权利要求30所述的AAV,其特征在于,所述病毒具有SEQ ID NO:3所示的核酸序列。
32.如权利要求30所述的AAV,其特征在于,所述AAV还包含具有AAV末端反向重复的小基因和操作性连接于调节序列的异源基因,所述调节序列引导该异源基因在宿主细胞中表达。
33.一种含有AAV9/HU.14蛋白或其片段的蛋白,所述AAV9/HU.14蛋白或其片段选自:
(a)选自下列的AAV9/HU.14衣壳蛋白或其片段:
vp1衣壳蛋白,SEQ ID NO:123的氨基酸(aa)1-736;
vp2衣壳蛋白,SEQ ID NO:123的aa 138-736;
vp3衣壳蛋白,SEQ ID NO:123的aa 203-736;
选自下列的含有超变区(HVR)1-12的片段或其较小的片段:aa146-152、aa182-187、aa262-264、aa263-266、aa263-266、aa381-383、383-385、aa450-474、aa451-475、aa490-495、aa491-496、aa500-504、aa501-505、aa514-522、aa533-554、aa534-555、aa581-594、aa583-596、aa658-667、aa660-669、aa705-719、aa707-723;
aa24-42,aa25-28、aa81-85、aa133-165、aa134-165、aa137-143、aa154-156、aa194-208、aa261-274、aa262-274、aa171-173、aa185-198、aa413-417、aa449-478、aa494-525、aa534-571、aa581-601、aa660-671、aa709-723;和
aa1-184、aa199-259、aa274-446、aa603-659、aa670-706、aa724-736、aa185-198、aa260-273、aa447-477、aa495-602、aa603-659、aa660-669与aa707-723,其中,氨基酸数是SEQ ID NO:4所示AAV2衣壳和SEQ ID NO:123所示AAV9/HU.14衣壳中相应区域的氨基酸数。
34.一种含有如权利要求33a所述的一种或多种AAV9/HU.14衣壳蛋白片段的人工腺伴随病毒(AAV)衣壳蛋白。
35.一种含有如权利要求34所述人工衣壳蛋白的重组腺伴随病毒(AAV)。
36.一种含有编码如权利要求34所述蛋白的核酸序列的分子。
37.如权利要求36所述的分子,其特征在于,所述核酸序列选自:
vp1,核苷酸1-2211;
vp2,核苷酸2532-2211;和
vp3,核苷酸2730-2211;
其中所述核苷酸数是SEQ ID NO:3所示AAV9/HU.14的核苷酸数。
38.如权利要求37所述的分子,其特征在于,所述分子含有编码AAV衣壳蛋白和功能性AAV rep蛋白的AAV序列。
39.如权利要求37所述的分子,其特征在于,所述分子是质粒。
40.一种产生含有AAV衣壳的重组腺伴随病毒(AAV)的方法,所述方法包括培养宿主细胞的步骤,所述宿主细胞含有:(a)编码AAV衣壳蛋白的分子;(b)功能性rep基因;(c)含有AAV末端反向重复(ITR)的小基因和转基因;和(d)允许将所述小基因包装进AAV衣壳蛋白的足够辅助功能物,其中所述宿主细胞含有如权利要求37所述的分子。
41.一种用如权利要求30所述的腺伴随病毒转染的宿主细胞。
42.一种用如权利要求33所述分子感染的宿主细胞。
43.一种含有如权利要求30所述AAV和生理相容运载体的组合物。
44.一种含有如权利要求36所述分子和生理相容运载体的组合物。
45.一种将转基因递送至细胞的方法,所述方法包括将该细胞与如权利要求30所述AAV接触的步骤,其中所述rAAV含有转基因。
46.一种非天然腺伴随病毒(AAV),所述AAV包含具有选自下列的氨基酸序列的AAV衣壳:
进化支E的30.10/pi.1(SEQ ID NO:93)、30.12/pi.2(SEQ ID NO:95)、30.19/pi.3(SEQ ID NO:94)、LG-4/rh.38(SEQ ID NO:86)、LG-10/rh.40(SEQ ID NO:92)、N721-8/rh.43(SEQ ID NO:163)、1-8/rh.49(SEQ ID NO:103)、2-4/rh.50(SEQ IDNO:108)、2-5/rh.51(SEQ ID NO:104)、3-9/rh.52(SEQ ID NO:96)、3-11/rh.53(SEQID NO:97)、5-3/rh.57(SEQ ID NO:105)、5-22/rh.58(SEQ ID NO:58)、2-3/rh.61(SEQID NO:107)、4-8/rh.64(SEQ ID NO:99)、3.1/hu.6(SEQ ID NO:84)、33.12/hu.17(SEQID NO:83)、106.1/hu.37(SEQ ID NO:88)、LG-9/hu.39(SEQ ID NO:102)、114.3/hu.40(SEQ ID NO:87)、127.2/hu.41(SEQ ID NO:91)、127.5/hu.42(SEQ IDNO:85)、hu.66(SEQ ID NO:197)、修饰的rh.2(SEQ ID NO:231)、和hu.67(SEQ IDNO:198);
进化支B的2-15/rh.62(SEQ ID NO:114)、1-7/rh.48(SEQ ID NO:115)、4-9/rh.54(SEQ ID NO:116)、4-19/rh.55(SEQ ID NO:117)、修饰的cy.5(SEQ IDNO:227)、修饰的rh.13(SEQ ID NO:228)和修饰的rh.37(SEQ ID NO:229);进化支B的52/hu.19(SEQ ID NO:133)、52.1/hu.20(SEQ ID NO:134)、54.5/hu.23(SEQID NO:137)、54.2/hu.22(SEQ ID NO:138)、54.7/hu.24(SEQ ID NO:136)、54.1/hu.21(SEQ ID NO:135)、54.4R/hu.27(SEQ ID NO:140)、46.2/hu.28(SEQ ID NO:130)、46.6/hu.29(SEQ ID NO:132)、修饰的hu.29(SEQ ID NO:225)、172.1/hu.63(SEQ IDNO:195)、172.2/hu.64(SEQ ID NO:196)、24.5/hu.13(SEQ ID NO:129)、145.6/hu.56(SEQ ID NO:192)、hu.57(SEQ ID NO:193)、136.1/hu.49(SEQ ID NO:189)、156.1/hu.58(SEQ ID NO:194)、72.2/hu.34(SEQ ID NO:125)、72.3/hu.35(SEQ IDNO:164)、129.1/hu.45(SEQ ID NO:127)、130.1/hu.47(SEQ ID NO:128)、140.1/hu.51(SEQ ID NO:190)和140.2/hu.52(SEQ ID NO:191);
进化支A的128.1/hu.43(SEQ ID NO:160)、修饰的hu.43(SEQ ID NO:236)、128.3/hu.44(SEQ ID NO:158)、hu.46(SEQ ID NO:159)、修饰的hu.46(SEQ IDNO:224)和130.4/hu.48(SEQ ID NO:157);
进化支C的3.1/hu.9(SEQ ID NO:155)、16.8/hu.10(SEQ ID NO:156)、16.12/hu.11(SEQ ID NO:153)、145.1/hu.53(SEQ ID NO:186)、145.6/hu.55(SEQ IDNO:187)、145.5/hu.54(SEQ ID NO:188)、7.3/hu.7(SEQ ID NO:150)、修饰的hu.7(SEQ ID NO:226)、33.4/hu.15(SEQ ID NO:147)、33.8/hu.16(SEQ ID NO:148)、58.2/hu.25(SEQ ID NO:146)、161.10/hu.60(SEQ ID NO:184)、H-5/hu.3(SEQ IDNO:145)、H-1/hu.1(SEQ ID NO:144)、hu.18(SEQ ID NO:52和149)和161.6/hu.61(SEQ ID NO:185);
进化支F的hu.31(SEQ ID NO:121)和hu.32(SEQ ID NO:122);和
rh.59(SEQ ID NO:110)和rh.60(SEQ ID NO:120);
或其独特的功能性片段。
47.如权利要求46所述的AAV,其特征在于,所述片段选自:
选自下列的含有超变区(HVR)1-12的片段或其较小的片段:aa146-152、aa182-187、aa262-264、aa263-266、aa263-266、aa381-383、383-385、aa450-474、aa451-475、aa490-495、aa491-496、aa500-504、aa501-505、aa514-522、aa533-554、aa534-555、aa581-594、aa583-596、aa658-667、aa660-669、aa705-719、aa707-723;
aa24-42、aa25-28、aa81-85、aa133-165、aa134-165、aa137-143、aa154-156、aa194-208、aa261-274、aa262-274、aa171-173、aa185-198、aa413-417、aa449-478、aa494-525、aa534-571、aa581-601、aa660-671、aa709-723;和
aa1-184、aa199-259、aa274-446、aa603-659、aa670-706、aa724-736、aa185-198、aa260-273、aa447-477、aa495-602、aa603-659、aa660-669和aa707-723,
其中氨基酸数是SEQ ID NO:4所示AAV2衣壳和以下衣壳中相应区域的氨基酸数:进化支E的30.10/pi.1(SEQ ID NO:93)、30.12/pi.2(SEQ ID NO:95)、30.19/pi.3(SEQ ID NO:94)、LG-4/rh.38(SEQ ID NO:86)、LG-10/rh.40(SEQ IDNO:92)、N721-8/rh.43(SEQ ID NO:163)、1-8/rh.49(SEQ ID NO:103)、2-4/rh.50(SEQ ID NO:108)、2-5/rh.51(SEQ ID NO:104)、3-9/rh.52(SEQ ID NO:96)、3-11/rh.53(SEQ ID NO:97)、5-3/rh.57(SEQ ID NO:105)、5-22/rh.58(SEQ IDNO:58)、修饰的rh.58(SEQ ID NO:232)、2-3/rh.61(SEQ ID NO:107)、4-8/rh.64(SEQ ID NO:99)、修饰的rh.64(SEQ ID NO:233)、3.1/hu.6(SEQ ID NO:84)、33.12/hu.17(SEQ ID NO:83)、106.1/hu.37(SEQ ID NO:88)、LG-9/hu.39(SEQ IDNO:102)、114.3/hu.40(SEQ ID NO:87)、127.2/hu.41(SEQ ID NO:91)、127.5/hu.42(SEQ ID NO:85)、hu.66(SEQ ID NO:197)、修饰的rh.2(SEQ ID NO:231)和hu.67(SEQ ID NO:198);
进化支B的2-15/rh.62(SEQ ID NO:114)、1-7/rh.48(SEQ ID NO:115)、4-9/rh.54(SEQ ID NO:116)、4-19/rh.55(SEQ ID NO:117)、修饰的cy.5(SEQ IDNO:227)、修饰的rh.13(SEQ ID NO:228)、修饰的rh.37(SEQ ID NO:229);进化支B的52/hu.19(SEQ ID NO:133)、52.1/hu.20(SEQ ID NO:134)、54.5/hu.23(SEQID NO:137)、54.2/hu.22(SEQ ID NO:138)、54.7/hu.24(SEQ ID NO:136)、54.1/hu.21(SEQ ID NO:135)、54.4R/hu.27(SEQ ID NO:140)、46.2/hu.28(SEQ ID NO:130)、46.6/hu.29(SEQ ID NO:132)、修饰的hu.29(SEQ ID NO:225)、172.1/hu.63(SEQ IDNO:195)、172.2/hu.64(SEQ ID NO:196)、24.5/hu.13(SEQ ID NO:129)、145.6/hu.56(SEQ ID NO:192)、hu.57(SEQ ID NO:193)、136.1/hu.49(SEQ ID NO:189)、156.1/hu.58(SEQ ID NO:194)、72.2/hu.34(SEQ ID NO:125)、72.3/hu.35(SEQ IDNO:164)、129.1/hu.45(SEQ ID NO:127)、130.1/hu.47(SEQ ID NO:128)、140.1/hu.51(SEQ ID NO:190)和140.2/hu.52(SEQIDNO:191);
进化支A的128.1/hu.43(SEQ ID NO:160)、修饰的hu.43(SEQ ID NO:236)、128.3/hu.44(SEQ ID NO:158)、hu.46(SEQ ID NO:159)、修饰的hu.46(SEQ IDNO:224)和130.4/hu.48(SEQ ID NO:157);
进化支C的3.1/hu.9(SEQ ID NO:155)、16.8/hu.10(SEQ ID NO:156)、16.12/hu.11(SEQ ID NO:153)、145.1/hu.53(SEQ ID NO:186)、145.6/hu.55(SEQ IDNO:187)、145.5/hu.54(SEQ ID NO:188)、7.3/hu.7(SEQ ID NO:150)、修饰的hu.7([SEQ ID NO:226]、33.4/hu.15(SEQ ID NO:147)、33.8/hu.16(SEQ ID NO:148)、58.2/hu.25(SEQ ID NO:146)、161.10/hu.60(SEQ ID NO:184)、H-5/hu.3(SEQ IDNO:145)、H-1/hu.1(SEQ ID NO:144)、hu.18(SEQ ID NO:149)和161.6/hu.61(SEQID NO:185);
进化支F的hu.31(SEQ ID NO:121)和hu.32(SEQ ID NO:122);和
rh.59(SEQ ID NO:110)和rh.60(SEQ ID NO:120)。
48.一种含有如权利要求47a所述的一种或多种AAV衣壳蛋白片段的人工腺伴随病毒(AAV)衣壳蛋白。
49.一种含有如权利要求48所述人工衣壳的重组腺伴随病毒(AAV)。
50.一种含有编码AAV衣壳蛋白的异源核酸序列的分子,其中所述核酸序列选自:
进化支E的30.10/pi.1(SEQ ID NO:28)、30.12/pi.2(SEQ ID NO:30)、30.19/pi.3(SEQ ID NO:29)、LG-4/rh.38(SEQ ID NO:7)、LG-10/rh.40(SEQ IDNO:14)、N721-8/rh.43(SEQ ID NO:43、1-8/rh.49(SEQ ID NO:25)、2-4/rh.50(SEQID NO:23)、2-5/rh.51(SEQ ID NO:22)、3-9/rh.52(SEQ ID NO:18)、3-11/rh.53(SEQID NO:17)、5-3/rh.57(SEQ ID NO:26)、5-22/rh.58(SEQ ID NO:27)、修饰的rh.58(SEQ ID NO:232)、2-3/rh.61(SEQ ID NO:21)、4-8/rh.64(SEQ ID NO:15)、3.1/hu.6(SEQ ID NO:5)、33.12/hu.17(SEQ ID N0:4)、106.1/hu.37(SEQ ID NO:10)、LG-9/hu.39(SEQ ID NO:24)、114.3/hu.40(SEQ ID NO:11)、127.2/hu.41(SEQ IDNO:6)、127.5/hu.42(SEQ ID NO:8)、hu.66(SEQ ID NO:173)、修饰的rh.2(SEQ IDNO:231)和hu.67(SEQ ID NO:174);
进化支D的2-15/rh.62(SEQ ID NO:33)、1-7/rh.48(SEQ ID NO:32)、4-9/rh.54(SEQ ID NO:40)、4-19/rh.55(SEQ ID NO:37和117)、修饰的cy.5(SEQ IDNO:227)、修饰的rh.13(SEQ ID NO:228)和修饰的rh.37(SEQ ID NO:229);
进化支B的52/hu.19(SEQ ID NO:62和133)、52.1/hu.20(SEQ ID NO:63和134)、54.5/hu.23(SEQ ID NO:60)、54.2/hu22(SEQ ID NO:67)、54.7/hu.24(SEQ IDNO:66)、54.1/hu.21(SEQ ID NO:65)、54.4R/hu.27(SEQ ID NO:64)、46.2/hu.28(SEQ ID NO:68)、46.6/hu.29(SEQ ID NO:69)、修饰的hu.29(SEQ ID NO:225);
进化支A的128.1/hu.43(SEQ ID NO:80)、128.3/hu.44(SEQ ID NO:81)、hu.46(SEQ ID NO:82)和130.4/hu.48(SEQ ID NO:78);
进化支C的3.1/hu.9(SEQ ID NO:58)、16.8/hu.10(SEQ ID NO:56)、16.12/hu.11(SEQ ID NO:57)、145.1/hu.53(SEQ ID NO:176)、145.6/hu.55(SEQ IDNO:178)、145.5/hu.54(SEQ ID NO:177)、7.3/hu.7(SEQ ID NO:55)、修饰的hu.7(SEQ ID NO:226)、33.4/hu.15(SEQ ID NO:50)、33.8/hu.16(SEQ ID NO:51)、58.2/hu.25(SEQ ID NO:49)、161.10/hu.60(SEQ ID NO:170)、H-5/hu.3(SEQ IDNO:44)、hu.18(SEQ ID NO:149)、H-1/hu.1(SEQ ID NO:46)和161.6/hu.61(SEQ IDNO:174);
进化支F的hu.14/AAV9(SEQ ID NO:3)、hu.31(SEQ ID NO:1)和hu.32(SEQID NO:2);
rh.59(SEQ ID NO:49)和rh.60(SEQ ID NO:31);
或其独特的功能性片段。
51.如权利要求50所述的分子,其特征在于,所述分子含有编码AAV衣壳蛋白的AAV序列和功能性AAV rep蛋白。
52.如权利要求50所述的分子,其特征在于,所述分子是质粒。
53.一种产生含有AAV衣壳的重组腺伴随病毒(AAV)的方法,所述方法包括培养宿主细胞的步骤,所述宿主细胞含有:(a)编码AAV衣壳蛋白的分子;(b)功能性rep基因;(c)含有AAV末端反向重复(ITR)的小基因和转基因;和(d)允许将所述小基因包装进AAV衣壳蛋白的足够辅助功能物,其中所述宿主细胞含有如权利要求50所述的分子。
54.一种用权利要求30所述腺伴随病毒转染的宿主细胞。
55.一种用权利要求50所述分子转染的宿主细胞。
56.一种含有如权利要求47所述AAV和生理相容运载体的组合物。
57.一种含有如权利要求50所述分子和生理相容运载体的组合物。
58.一种将转基因递送至细胞的方法,所述方法包括将该细胞与权利要求47所述AAV接触的步骤,其中所述rAAV含有转基因。
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CN106062200A (zh) * | 2014-02-17 | 2016-10-26 | 伦敦国王学院 | 腺相关病毒载体 |
CN106062200B (zh) * | 2014-02-17 | 2020-06-23 | 伦敦国王学院 | 腺相关病毒载体 |
US11802293B2 (en) | 2014-02-17 | 2023-10-31 | King's College London | Adeno-associated virus vector |
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