AR011432A1 - Metodos para una transformacion mediada por agrobacterium - Google Patents

Metodos para una transformacion mediada por agrobacterium

Info

Publication number
AR011432A1
AR011432A1 ARP980100307A ARP980100307A AR011432A1 AR 011432 A1 AR011432 A1 AR 011432A1 AR P980100307 A ARP980100307 A AR P980100307A AR P980100307 A ARP980100307 A AR P980100307A AR 011432 A1 AR011432 A1 AR 011432A1
Authority
AR
Argentina
Prior art keywords
agrobacterium
embryos
gene
methods
corn
Prior art date
Application number
ARP980100307A
Other languages
English (en)
Original Assignee
Pioneer Hi Bred Int
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Pioneer Hi Bred Int filed Critical Pioneer Hi Bred Int
Publication of AR011432A1 publication Critical patent/AR011432A1/es

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8202—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by biological means, e.g. cell mediated or natural vector
    • C12N15/8205—Agrobacterium mediated transformation

Abstract

Métodos para mejorar la frecuencia de transformacion mediada por Agrobacterium de embriones de maíz. El método preferido para transformar elmaíz utilizando Agrobacterium comprende los pasos de: poner por lo menos un embrion inmadurode una planta de maíz en contacto con Agrobacterium capaz detransferir por lo menos un gen a dicho embrion; co-cultivar los embriones con agrobacterium; cultivar los embriones en un medio que contiene sales N6, unantibiotico capaz de inhibir elcreci miento de Agrobacterium y un agente de seleccion para seleccinar los embriones que expresan el gen; y regenerarplantas que expresan el gen.
ARP980100307A 1997-01-24 1998-01-23 Metodos para una transformacion mediada por agrobacterium AR011432A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US08/788,018 US5981840A (en) 1997-01-24 1997-01-24 Methods for agrobacterium-mediated transformation

Publications (1)

Publication Number Publication Date
AR011432A1 true AR011432A1 (es) 2000-08-16

Family

ID=25143194

Family Applications (1)

Application Number Title Priority Date Filing Date
ARP980100307A AR011432A1 (es) 1997-01-24 1998-01-23 Metodos para una transformacion mediada por agrobacterium

Country Status (6)

Country Link
US (2) US5981840A (es)
EP (1) EP0971578A1 (es)
AR (1) AR011432A1 (es)
AU (1) AU727849B2 (es)
CA (1) CA2278618A1 (es)
WO (1) WO1998032326A2 (es)

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