AR016179A1 - Metodo para producir semilla hibrida. - Google Patents

Metodo para producir semilla hibrida.

Info

Publication number
AR016179A1
AR016179A1 ARP990100725A ARP990100725A AR016179A1 AR 016179 A1 AR016179 A1 AR 016179A1 AR P990100725 A ARP990100725 A AR P990100725A AR P990100725 A ARP990100725 A AR P990100725A AR 016179 A1 AR016179 A1 AR 016179A1
Authority
AR
Argentina
Prior art keywords
plant
parent
female
male
pollination
Prior art date
Application number
ARP990100725A
Other languages
English (en)
Original Assignee
Syngenta Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GBGB9803659.3A external-priority patent/GB9803659D0/en
Priority claimed from GBGB9805669.0A external-priority patent/GB9805669D0/en
Application filed by Syngenta Ltd filed Critical Syngenta Ltd
Publication of AR016179A1 publication Critical patent/AR016179A1/es

Links

Classifications

    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01HNEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00Processes for modifying genotypes ; Plants characterised by associated natural traits
    • A01H1/02Methods or apparatus for hybridisation; Artificial pollination ; Fertility
    • A01H1/022Genic fertility modification, e.g. apomixis
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y302/00Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01031Beta-glucuronidase (3.2.1.31)
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8222Developmentally regulated expression systems, tissue, organ specific, temporal or spatial regulation
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8237Externally regulated expression systems
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8287Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for fertility modification, e.g. apomixis
    • C12N15/8289Male sterility
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8287Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for fertility modification, e.g. apomixis
    • C12N15/829Female sterility
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10Transferases (2.)
    • C12N9/1048Glycosyltransferases (2.4)
    • C12N9/1051Hexosyltransferases (2.4.1)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/16Hydrolases (3) acting on ester bonds (3.1)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/24Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405Glucanases
    • C12N9/2434Glucanases acting on beta-1,4-glucosidic bonds
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y301/00Hydrolases acting on ester bonds (3.1)
    • C12Y301/01Carboxylic ester hydrolases (3.1.1)
    • C12Y301/01011Pectinesterase (3.1.1.11)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y301/00Hydrolases acting on ester bonds (3.1)
    • C12Y301/27Endoribonucleases producing 3'-phosphomonoesters (3.1.27)
    • C12Y301/27003Ribonuclease T1 (3.1.27.3)

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Botany (AREA)
  • Developmental Biology & Embryology (AREA)
  • Environmental Sciences (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)

Abstract

Se describen métodos para preparar semillas híbridas que resuelven el problema de preparar semilla híbrida donde la auto polinizacion de cada progenitor escontrolada. La restauracion de fertilidad en las plantas progenitoras permite el mantenimientode las líneas progenitoras. Una planta progenitora masculina,con fertilidad masculina y con esterilidad femenina homocigota recesiva y una planta progenitora femenina, con esterilidad masculina homocigota recesiva y confertilidad femenina, seintersi embran para permitir la polinizacion cruzada y se obtiene la semilla producida por el cruzamiento. El ADN génico de cada plantaprogenitora tiene integrado en sí mismo una construccion génica que comprende una secuencia promotora quecorresponde a la presencia o ausencia de un inductorquímico exogeno, opcionalmente operativamente ligado a uno o más potenciadores de la traduccion o secuencias de intrones, operativamente ligadas a un gen quecompletamente restaura la fertilidad decada planta progen itora. El gen es expresado por la aplicacion a la planta de un inductor químico externo permitiendoasí la auto polinizacion de cada progenitor. Los métodos hacen que no sea necesario la siembra de bloques separados para plantasmasculinas y plantasfe meninas, maximizando la oportunidad de polinizacion cruzada y evitando la necesidad de aplicar herbicidas para remover las plantas progenitoras masculinasluego de la fertilizacion de la planta progenitora femenina.
ARP990100725A 1998-02-20 1999-02-23 Metodo para producir semilla hibrida. AR016179A1 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GBGB9803659.3A GB9803659D0 (en) 1998-02-20 1998-02-20 Hybrid seed production
GBGB9805669.0A GB9805669D0 (en) 1998-03-17 1998-03-17 Hybrid seed production

Publications (1)

Publication Number Publication Date
AR016179A1 true AR016179A1 (es) 2001-06-20

Family

ID=26313162

Family Applications (1)

Application Number Title Priority Date Filing Date
ARP990100725A AR016179A1 (es) 1998-02-20 1999-02-23 Metodo para producir semilla hibrida.

Country Status (20)

Country Link
US (1) US6683230B1 (es)
EP (2) EP1054985B1 (es)
JP (1) JP2002504343A (es)
KR (1) KR20010074432A (es)
CN (1) CN1206360C (es)
AR (1) AR016179A1 (es)
AT (1) ATE554173T1 (es)
AU (1) AU752892B2 (es)
BR (1) BR9908126A (es)
CA (1) CA2319110C (es)
CY (1) CY1113273T1 (es)
DK (1) DK1054985T3 (es)
DZ (1) DZ2726A1 (es)
EG (1) EG22268A (es)
ES (1) ES2385307T3 (es)
HU (1) HU228267B1 (es)
IL (1) IL137972A0 (es)
MY (1) MY133111A (es)
PT (1) PT1054985E (es)
WO (1) WO1999042598A2 (es)

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BR0307965A (pt) 2002-02-26 2005-02-01 Syngenta Ltd Processo para produzir seletivamente plantas masculinas ou femininas estéries
EP1485491A1 (en) * 2002-03-19 2004-12-15 Universite Libre De Bruxelles Poison/antidote genetic systems for the selection of genetically modified eucaryote cells or organisms
CN104313049A (zh) * 2003-12-16 2015-01-28 先锋高级育种国际公司 显性基因抑制性转基因及其使用方法
WO2008021397A1 (en) * 2006-08-15 2008-02-21 University Of Florida Research Foundation, Inc. Materials and methods for improving quality and characteristics of grasses
US8222388B2 (en) * 2006-11-22 2012-07-17 Ceres, Inc. Broadly expressing regulatory regions
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GB201015791D0 (en) 2010-09-20 2010-10-27 Syngenta Ltd Improved method and machine for use thereof
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CN104837334B (zh) * 2013-05-23 2017-06-23 深圳市作物分子设计育种研究院 植物新型保持系及不育系建立及其用途
WO2015101243A1 (zh) * 2014-01-02 2015-07-09 北京思创达科技有限公司 雌性不育系的繁殖及杂交制种技术
CN103733981B (zh) * 2014-01-15 2015-04-08 新疆农垦科学院 一种杂交小麦设施隔离制种方法
CN103947546B (zh) * 2014-05-15 2016-05-18 石河子农业科学研究院 一种甜菜单粒高抗成对不育系的育种方法
WO2016073559A1 (en) * 2014-11-05 2016-05-12 The Regents Of The University Of California Methods for autocatalytic genome editing and neutralizing autocatalytic genome editing
CN107306537B (zh) * 2017-07-27 2020-08-11 河北省农林科学院经济作物研究所 一种番茄取种的方法
CN110199803B (zh) * 2019-06-25 2022-10-25 华南农业大学 两系不育系水稻育性转换有效低温累积效应的鉴定方法
CN111139257B (zh) * 2020-01-15 2022-10-14 南京农业大学 一种梨pmei蛋白体外表达的方法及其应用
CN112715349B (zh) * 2020-12-31 2021-07-16 云南农业大学 一种多年生型水稻雌性核不育系的选育方法
CN113475385A (zh) * 2021-07-20 2021-10-08 贺州市农业科学院 一种具有叶片标记性状的三系杂交水稻不育系的选育方法
CN116018954B (zh) * 2022-10-19 2023-11-17 江苏省农业科学院 一种饲草高粱-巨大芽孢杆菌共生体良种繁育方法

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Also Published As

Publication number Publication date
US6683230B1 (en) 2004-01-27
HU228267B1 (en) 2013-02-28
CA2319110C (en) 2011-01-04
JP2002504343A (ja) 2002-02-12
HUP0100655A2 (hu) 2001-06-28
CA2319110A1 (en) 1999-08-26
PT1054985E (pt) 2012-07-03
DZ2726A1 (fr) 2003-09-01
DK1054985T3 (da) 2012-07-16
ATE554173T1 (de) 2012-05-15
WO1999042598A3 (en) 1999-10-21
ES2385307T3 (es) 2012-07-20
AU2179099A (en) 1999-09-06
MY133111A (en) 2007-10-31
CN1298450A (zh) 2001-06-06
EP2258856A1 (en) 2010-12-08
AU752892B2 (en) 2002-10-03
IL137972A0 (en) 2001-10-31
EP1054985A2 (en) 2000-11-29
CY1113273T1 (el) 2016-04-13
HUP0100655A3 (en) 2003-04-28
WO1999042598A2 (en) 1999-08-26
EP1054985B1 (en) 2012-04-18
KR20010074432A (ko) 2001-08-04
EG22268A (en) 2002-11-30
CN1206360C (zh) 2005-06-15
BR9908126A (pt) 2000-10-24

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