CN110799526A

CN110799526A - 通过使用abc转运体序列来增加植物生长和产量

Info

Publication number: CN110799526A
Application number: CN201880043122.5A
Authority: CN
Inventors: M·贝其曼
Original assignee: Benson Hill Inc
Current assignee: Benson Hill Inc
Priority date: 2017-05-22
Filing date: 2018-05-21
Publication date: 2020-02-14
Also published as: BR112019024479A2; WO2018215915A1; EP3630801A1; US20200208168A1; US11535857B2; CA3064511A1

Abstract

本文提供用于改善植物生长的组合物和方法。也提供编码ABC转运体蛋白的多核苷酸、包含ABC转运体蛋白的多肽、用于表达感兴趣的基因的表达构建体，包含所述多核苷酸、多肽和表达构建体的植物，以及制备转基因植物的方法；所述感兴趣的基因的表达可以改善农艺性质，包括但不限于作物产量、生物胁迫和非生物胁迫耐受性、早期萌发势。

Description

通过使用ABC转运体序列来增加植物生长和产量

技术领域

本发明涉及通过在植物中表达ABC转运体基因来增加植物生长和产量的组合物和方法。

背景技术

世界人口的持续增长和农业上可用的可耕种土地的供应萎缩激励着人们研究开发具有更高的生物质和产量的植物。作物和园艺改良的常规手段是利用选择性育种技术来鉴定具有所需特性的植物。然而，这种选择性育种技术具有多种缺点，即，这些技术通常要耗费大量劳动力，且所得植物往往含有异源遗传组分，其不总是能从亲本植物带来所需的性状。分子生物学的发展提供了精确地改良植物种质的手段。植物的基因工程使得人们可以分离和操作遗传物质(通常为DNA或RNA的形式)，随后将该遗传物质导入植物中。这种技术能够产生出具有各种改良的经济、农艺或园艺性状的作物或植物。

感兴趣的性状包括植物生物质和产量。产量一般定义为来自作物的经济价值的可测量产出。这可以就数量和/或品质方面进行定义。产量直接取决于多种因素，例如器官的数量和尺寸、植物构筑型(例如枝条的数量)、种子生产、叶衰老等。根发育、养分摄取、胁迫耐受性、光合碳同化速率和早期萌发势也可以是决定产量的重要因素。因此，优化上述因素可以有助于增加作物产量。

种子产量的增加是特别重要的性状，因为许多植物的种子对于人和动物的消费而言很重要。玉米、水稻、小麦、油菜(canola)和大豆等作物占人类总热量摄入的一半多，无论是通过直接消费种子本身或是通过消费建立在加工种子基础上的肉产品。它们也是糖、油及工业加工中所用的多种代谢物的来源。种子含有胚(新苗和新根的起源)和胚乳(萌发期间及幼苗早期生长期间用于胚生长的养分来源)。种子的发育牵涉到许多基因，并且需要代谢物从根、叶和茎转移至正在生长的种子中。尤其，胚乳对碳水化合物、油和蛋白质的代谢前体进行同化，并将它们合成为贮藏大分子以灌满籽粒。植物生物质的增加对于苜蓿、青贮玉米和干草之类的饲料作物很重要。许多基因参与到有助于植物生长和发育的代谢途径中。调节植物中一个或多个这类基因的表达可产生相对于对照植物具有更好的生长和发育的植物，但往往也会产生相对于对照植物具有较差的生长和发育。因此，需要改善植物生长和发育的方法。

发明内容

本申请提供用于调节植物中的基因表达的组合物和方法。该方法增加植物生长，带来更高的作物产量。该方法包括增加感兴趣的植物中至少一个ABC转运体基因的表达。本发明也包括构建体，该构建体包含与ABC转运体编码序列操作性连接、驱动植物细胞中的表达的启动子。组合物还包含具有增加的ABC转运体序列表达的植物、植物种子、植物器官、植物细胞及其它植物部分。本发明包括可用于增加植物中ABC转运体基因的表达的方法。该ABC转运体基因可以是天然序列，或者可以是对于感兴趣的植物而言是异源的序列。

本发明的实施方式包括：

1.一种用于增加作物产量的方法，包括用至少一个ABC转运体蛋白编码序列转化植物。

2.实施方式1的方法，其中，所述ABC转运体蛋白编码序列包含SEQ ID NO:1，或者编码选自SEQ ID NO:2和15-103的蛋白质。

3.实施方式1的方法，其中，所述ABC转运体蛋白编码序列编码与选自SEQ ID NO:2和15-103的序列具有至少80％、90％、95％、96％、97％、98％或99％的序列相同性、且具有ABC转运体功能的蛋白质。

4.实施方式1的方法，其中，所述ABC转运体蛋白编码序列编码相对于选自SEQ IDNO:2和15-103的序列具有至少80％、85％、90％、95％、96％、97％、98％或99％的序列正值率、且具有ABC转运体功能的蛋白质。

5.一种植物，在其基因组中稳定地引入了与ABC转运体蛋白编码序列操作性连接、驱动植物中的表达的启动子，其中，所述启动子对于所述ABC转运体蛋白编码序列而言是异源的。

6.实施方式5的植物，其中，所述ABC转运体蛋白编码序列包含SEQ ID NO:1，或者编码选自SEQ ID NO:2和15-103的蛋白质。

7.实施方式5的植物，其中，所述ABC转运体蛋白编码序列编码与选自SEQ ID NO:2和15-103的序列具有至少80％、85％、90％、95％、96％、97％、98％或99％的序列相同性、且具有ABC转运体功能的蛋白质。

8.实施方式5的植物，其中，所述ABC转运体蛋白编码序列编码相对于选自SEQ IDNO:2和15-103的序列具有至少80％、85％、90％、95％、96％、97％、98％或99％的序列正值率、且具有ABC转运体功能的蛋白质。

9.一种转化的种子，其是实施方式5～8中任一项的植物的转化的种子。

10.实施方式5～8中任一项的植物，其中，所述植物是单子叶植物。

11.实施方式10的植物，其中，所述植物来自玉米属、稻属、小麦属、高粱属、黑麦属、穇属、狗尾草属、甘蔗属、芒属、黍属、狼尾草属、大黍属、椰子属、凤梨属、芭蕉属、油棕属、燕麦属或大麦属。

12.实施方式5～8中任一项的植物，其中，所述植物是双子叶植物。

13.实施方式12的植物，其中，所述植物来自大豆属、芸薹属、苜蓿属、向日葵属、红花属、烟草属、茄属、棉属、番薯属、木薯属、咖啡属、柑橘属、可可属、山茶属、鳄梨属、榕属、番石榴属、杧果属、木犀榄属、番木瓜属、腰果属、澳洲坚果属、李属、甜菜属、杨属或桉属。

14.实施方式5～8中任一项的植物，其中，所述植物相对于对照植物表现出生长增加。

15.实施方式5～8中任一项的植物，其中，所述植物相对于对照植物表现出生物质产量增加。

16.实施方式5～8中任一项的植物，其中，所述植物相对于对照植物表现出种子产量增加。

17.实施方式1～4中任一项的方法，其中，所述ABC转运体蛋白编码序列自发育调控启动子表达。

18.实施方式17的方法，其中，所述发育调控启动子包含SEQ ID NO:3或SEQ IDNO:5。

19.实施方式1～18中任一项的方法，其还包括用至少一个其它蛋白编码序列转化植物。

20.实施方式19的方法，其中，所述至少一个其它蛋白编码序列选自SEQ ID NO:7和9，或者编码与选自SEQ ID NO:8和10的序列具有至少90％相同性的蛋白质。

21.实施方式19或20的方法，其中，所述至少一个其它蛋白编码序列编码选自SEQID NO:8和10的蛋白质。

22.实施方式5～8中任一项的植物，其中，所述驱动植物中的表达的启动子是发育调控启动子。

23.实施方式22的植物，其中，所述发育调控启动子包含SEQ ID NO:3或SEQ IDNO:5。

24.实施方式5的植物，在其基因组中稳定地引入了与第二蛋白编码序列操作性连接、驱动植物中的表达的第二启动子，其中，所述第二启动子对于所述第二蛋白编码序列而言是异源的。

25.实施方式24的植物，其中，所述第二蛋白编码序列选自SEQ ID NO:7和9，或者编码与选自SEQ ID NO:8和10的序列具有至少90％相同性的蛋白质。

26.实施方式24或25的植物，其中，所述第二蛋白编码序列编码选自SEQ ID NO:8和10的蛋白质。

27.一种DNA构建体，以操作性连接的方式包括：

a.在植物细胞中起作用的启动子，以及

b.编码ABC转运体蛋白的核酸序列。

28.实施方式27的DNA构建体，其中，所述编码ABC转运体蛋白的核酸序列包含SEQID NO:1，或者编码选自SEQ ID NO:2和15-103的蛋白质。

29.实施方式27或28的DNA构建体，其中，所述编码ABC转运体蛋白的核酸序列编码与选自SEQ ID NO:2和15-103的序列具有至少80％、85％、90％、95％、96％、97％、98％或99％的序列相同性、且具有ABC转运体功能的蛋白质。

30.实施方式27或28的DNA构建体，其中，所述编码ABC转运体蛋白的核酸序列编码相对于选自SEQ ID NO:2和15-103的序列具有至少80％、85％、90％、95％、96％、97％、98％或99％的序列正值率、且具有ABC转运体功能的蛋白质。

31.实施方式27或28的DNA构建体，其中，所述在植物细胞中起作用的启动子选自SEQ ID NO:3和5。

32.实施方式27～31中任一项的DNA构建体，其中，所述启动子对于所述编码ABC转运体蛋白的核酸序列而言是异源的。

33.一种用于增加作物产量的方法，包括调节植物中至少一个ABC转运体蛋白编码序列的表达。

34.实施方式33的方法，其中，对表达的所述调节包括增加植物中至少一个ABC转运体蛋白编码序列的表达。

35.实施方式34的方法，其中，对表达的所述增加包括增加所述植物中的天然ABC转运体序列的活性或增加所述植物中的天然ABC转运体蛋白编码序列的活性。

36.实施方式5～8中任一项的植物，其中，驱动植物中的表达的所述启动子在叶组织中有活性。

37.实施方式27～32中任一项的DNA构建体，其中，在植物细胞中起作用的所述启动子在叶组织中有活性。

具体实施方式

本申请提供用于增加作物生物质和产量的组合物和方法。该方法包括增加感兴趣的植物中至少一个ABC转运体基因的表达。作物产量是一种极为复杂的性状，是由作物植物在其所有发育阶段中的生长以及分配给植物可收获部分的植物资源而产生的结果。在包括但不限于玉米和大豆的一些作物中，主要可收获部分可以包括种子，而其余生物质(例如叶和茎)用于次要应用。在包括但不限于甘蔗和紫花苜蓿的其它作物中，植物的主要可收获部分由植物的茎或整个地上部分组成。在包括但不限于马铃薯和胡萝卜的其它作物中，植物的主要可收获部分位于地下。不论作物植物的收获部分如何，可收获生物质的积累都是由植物的生长以及分配给植物的收获部分的光合固定碳而产生的结果。植物生长可以通过调节一个或多个植物基因的表达来控制。这种调节可以改变一条或多条代谢途径的功能，从而有助于植物生长和可收获生物质的积累。

本发明的方法包括用于通过调节编码ABC转运体蛋白的一个或多个基因的表达来增加产量的植物生长控制。在一个优选实施方式中，相对于对照植物中的ABC转运体表达水平，将ABC转运体蛋白编码基因的表达上调，以使得ABC转运体表达相对于对照植物有所增加的植物中的可收获生物质增加。用于增加植物中的ABC转运体蛋白编码序列的活性或表达的任何方法都由本发明所涵盖。

本发明的组合物包括构建体，该构建体包含与在植物细胞中起作用的启动子操作性连接的、SEQ ID NO:1所示的编码序列或编码选自SEQ ID NO:2和15-103的蛋白质的编码序列或其变体。"启动子"意在表示能够驱动植物或植物细胞中序列表达的DNA调控区。应认识到，在已经鉴定了本文公开的ABC转运体蛋白序列的情况下，例如通过BLAST搜索、PCR试验等来分离和鉴定其它ABC转运体蛋白序列和编码ABC转运体蛋白序列的核苷酸序列都在本领域的水平内。

当本发明的编码序列组装在DNA构建体中以使得启动子操作性连接至感兴趣的编码序列时，其使得ABC转运体蛋白能够在用该DNA构建体稳定地转化的植物细胞中表达和积累。"操作性连接"意在表示两个或多个元件之间的功能性连接。例如，本发明的启动子和感兴趣的异源核苷酸之间的操作性连接是允许感兴趣的异源核苷酸序列表达的功能性连接。操作性连接的元件可以毗连或不毗连。用于指代两个蛋白质编码区的接合时，操作性连接意指这些编码区位于同一阅读框内。盒可以额外含有欲共转化至植物中的至少一个其它基因。或者，可以在多个表达盒或DNA构建体中提供其它基因。表达盒可以额外含有选择性标记基因。

通过该方式，在表达盒或表达构建体中和感兴趣的启动子序列(通常是异源启动子序列)一起提供编码本发明的ABC转运体蛋白的核苷酸序列，使其在感兴趣的植物中表达。"异源启动子序列"意在表示不是与ABC转运体蛋白编码核苷酸序列天然地操作性连接的序列。当ABC转运体蛋白编码核苷酸序列与启动子序列彼此异源时，ABC转运体蛋白编码核苷酸序列或异源启动子序列对于植物宿主而言可以是同源的、天然的、异源的或外来的。应认识到，启动子也可以驱动其同源或天然核苷酸序列的表达。在该情况下，转化的植物将具有表型变化。

本发明的多核苷酸和氨基酸序列的片段和变体也可以通过在植物细胞中可操作的启动子来表达。"片段"意指多核苷酸的一部分或氨基酸序列的一部分。"变体"意在表示基本上相似的序列。对于多核苷酸而言，变体包括：在5'和/或3'端具有缺失(即截短)的多核苷酸；在天然多核苷酸中的一个或多个内部位点上具有一个或多个核苷酸的缺失和/或添加的多核苷酸；和/或在天然多核苷酸中的一个或多个位点上具有一个或多个核苷酸的取代的多核苷酸。如本文所用，"天然"多核苷酸或多肽分别包括天然存在的核苷酸序列或氨基酸序列。一般来说，本发明的特定多核苷酸的变体与该特定多核苷酸具有至少约75％、80％、85％、90％、91％、92％、93％、94％、95％、96％、97％、98％、99％或更高的序列相同性，该序列相同性通过本文他处所述的序列比对程序和参数测得。本文所述的多核苷酸的片段和变体可编码保留ABC转运体功能的蛋白质。

"变体"氨基酸或蛋白质意在表示通过以下方式由天然氨基酸或蛋白质衍生的氨基酸或蛋白质：天然蛋白质N-末端和/或C-末端处的一个或多个氨基酸的缺失(所谓的截短)；天然蛋白质中的一个或多个内部位点上的一个或多个氨基酸的缺失和/或添加；或者天然蛋白质中的一个或多个位点上的一个或多个氨基酸的取代。本发明所涵盖的变体蛋白质具有生物活性，即，其继续具有天然蛋白质的所需生物活性，例如ATP的水解和无机离子的转运。天然多肽的生物活性变体与该天然序列的氨基酸序列具有至少约80％、85％、90％、91％、92％、93％、94％、95％、96％、97％、98％、99％或更高的序列相同性，该序列相同性通过本文所述的序列比对程序和参数测得。在一些实施方式中，变体多肽序列包含保守性氨基酸取代。将这种保守性氨基酸取代的数目与氨基酸相同性的数目之和除以感兴趣的序列中的氨基酸总数时，可以用来计算序列正值率(sequence positives)。序列正值率计算在NCBI BLAST服务器上进行，该NCBI BLAST服务器可以在万维网上通过blast.ncbi.nlm.nih.gov/Blast.cgi访问。本发明的蛋白质的生物活性变体可以与该蛋白质相差少至1～15个氨基酸残基、少至1～10个、如6～10个、少至5个、少至4个、3个、2个或者甚至1个氨基酸残基。

氨基酸通常可分类为脂族、含羟基或含硫/硒、环状、芳族、碱性、酸性及其酰胺。不受到理论的限制，在一些情况下，对于变体蛋白质序列的产生来说，保守性氨基酸取代可以优于非保守性氨基酸取代，这是因为保守性取代相比于非保守性取代可以更容易地让变体蛋白质保留其生物活性。编码在序列中具有一个或多个氨基酸取代的多肽的多核苷酸落入本发明的范围内。下表1提供了属于各种类别的氨基酸的例子的列表。

表1：氨基酸的类别

<u>氨基酸类别</u>	<u>示例氨基酸</u>
		脂族	Gly,Ala,Val,Leu,Ile
含羟基或含硫/硒	Ser,Cys,Thr,Met,Sec
		环状	Pro
芳族	Phe,Tyr,Trp
		碱性	His,Lys,Arg
酸性及其酰胺	Asp,Glu,Asn,Gln

变体序列也可以通过现有的已测序基因组数据库的分析来鉴定。通过该方式，可鉴定相应序列并用于本发明的方法。

比对序列的比较方法是本领域熟知的。因此，可采用数学算法确定任意两个序列的序列相同性百分数。这种数学算法的非限制性例子是Myers和Miller(1988)CABIOS 4:11-17的算法；Smith等(1981)Adv.Appl.Math.2:482的局部比对算法；Needleman和Wunsch(1970)J.Mol.Biol.48:443-453的全局比对算法；Pearson和Lipman(1988)Proc.Natl.Acad.Sci.85:2444-2448的局部搜索比对方法；Karlin和Altschul(1990)Proc.Natl.Acad.Sci.USA 87:2264-2268的在Karlin和Altschul(1993)Proc.Natl.Acad.Sci.USA 90:5873-5877中经过改良的算法。

这些数学算法的计算机实现可用于序列的比较以确定序列相同性。这类实施手段包括但不限于：PC/Gene程序(可获自加利福尼亚州山景城的智慧遗传(Intelligenetics)公司)中的CLUSTAL；GCG威斯康辛遗传学软件包(GCG Wisconsin Genetics SoftwarePackage)10版本(可获自美国加利福尼亚州圣地亚哥斯克兰顿路9685号克赛勒里公司(Accelrys Inc.))中的ALIGN程序(2.0版本)以及GAP、BESTFIT、BLAST、FASTA和TFASTA。使用这些程序的比对可以用默认参数来进行。CLUSTAL程序已由Higgins等(1988)Gene 73:237-244(1988)；Higgins等(1989)CABIOS 5:151-153；Corpet等(1988)Nucleic AcidsRes.16:10881-90；Huang等(1992)CABIOS 8:155-65；和Pearson等(1994)Meth.Mol.Biol.24:307-331详细描述。ALIGN程序基于上述Myers和Miller(1988)的算法。比较氨基酸序列时，可采用ALIGN程序以及PAM120权重残基表、缺口长度罚分12、缺口罚分4。Altschul等(1990)J.Mol.Biol.215:403的BLAST程序基于上述Karlin和Altschul(1990)的算法。可利用BLASTN程序进行BLAST核苷酸搜索(评分＝100，字长＝12)，以获得与编码本发明蛋白质的核苷酸序列同源的核苷酸序列。可利用BLASTX程序进行BLAST蛋白质搜索(评分＝50，字长＝3)，以获得与本发明蛋白质或多肽同源的氨基酸序列。为了进行缺口比对以用于比较目的，可如Altschul等(1997)Nucleic Acids Res.25:3389-3402所述(在BLAST2.0中)利用缺口BLAST。或者，可(在BLAST 2.0中)利用PSI-BLAST进行迭代搜索，其用来检测分子之间的远近关系。参见Altschul等(1997)同上。利用BLAST、缺口BLAST、PSI-BLAST时，可使用各程序(如核苷酸序列的BLASTN、蛋白质的BLASTX)的默认参数。参见www.ncbi.nlna.nih.gov。比对还可手工检查进行。

这类基因和编码区可以进行密码子优化，以便在感兴趣的植物中表达。"密码子优化的基因"是具有设计成模拟宿主细胞的偏好密码子使用频率的密码子使用频率的基因。核酸分子可以整体或部分进行密码子优化。因为任何一个氨基酸(除甲硫氨酸和色氨酸外)都由多个密码子编码，所以可以改变核酸分子的序列而又不改变所编码的氨基酸。密码子优化是指当在核酸水平上有一个或多个密码子发生改变时，使得氨基酸不变，而特定宿主生物体中的表达增加。本领域技术人员会认识到，密码子表及提供各种生物体的偏好信息的其它参考物是本领域已知的(参见例如Zhang等(1991)Gene 105:61-72；Murray等(1989)Nucl.Acids Res.17:477-508)。例如在美国专利第6,015,891号及其中引用的参考文献、以及WO 2012/142,371及其中引用的参考文献中，提供了优化核苷酸序列以便在植物中表达的方法。

本发明的核苷酸序列可以在重组多核苷酸中使用。“重组多核苷酸”包含在自然界中不会直接接合的两个或多个化学连接的核酸区段的组合。“直接接合”意指两个核酸区段紧邻并通过化学键彼此接合。在特定的实施方式中，重组多核苷酸包含感兴趣的多核苷酸或者其活性变体或片段，以使得另一化学连接的核酸区段位于感兴趣的多核苷酸的5’、3’或内部。或者，重组多核苷酸的化学连接的核酸区段可以通过序列的缺失来形成。另一化学连接的核酸区段或为了接合所连接的核酸区段而缺失的序列可以是任意长度，包括例如1、2、3、4、5、6、7、8、9、10、15、20或更多个核苷酸。本文公开了用于制备这类重组多核苷酸的各种方法，包括例如通过化学合成或通过由基因工程技术来操作分离的多核苷酸区段。在特定的实施方式中，重组多核苷酸可以包含重组DNA序列或重组RNA序列。“重组多核苷酸的片段”包含在自然界中不会直接接合的两个或多个化学连接的氨基酸区段的至少一种组合。

“改变”或“调节”基因的表达水平意指基因的表达上调或下调。应认识到，在一些情况下，通过增加编码ABC转运体蛋白的一个或多个基因的表达水平、即上调表达来增加植物生长和产量。同样，在一些情况下，通过减少编码ABC转运体蛋白的一个或多个基因的表达水平、即下调表达来增加植物生长和产量。因此，本发明涵盖编码ABC转运体蛋白的一个或多个基因的上调或下调。另外，该方法包括在感兴趣的植物中上调编码ABC转运体蛋白的至少一个基因以及下调编码第二ABC转运体蛋白的至少一个基因。调节转基因植物中编码ABC转运体蛋白的至少一个基因的浓度和/或活性，意指该浓度和/或活性相对于未导入本发明序列的天然对照植物、植物部分或细胞增加或减少至少约1％、约5％、约10％、约20％、约30％、约40％、约50％、约60％、约70％、约80％、约90％或更多。

应认识到，本发明的编码ABC转运体蛋白的基因的表达水平可以通过使用在植物细胞中起作用的一个或多个启动子来控制。感兴趣的ABC转运体蛋白编码基因的表达水平可以直接测定，例如通过检测植物中的ABC转运体基因转录物或所编码的蛋白质的水平来测定。这类检测的方法是本领域熟知的。例如，可以使用Northern印迹或定量逆转录酶-PCR(qRT-PCR)来检测转录物水平，而可以使用Western印迹、ELISA试验或酶试验来检测蛋白质水平。ABC转运体功能可以通过例如公知的ATP酶试验(Glavinas等2008Expert Opinion onDrug Metabolism&Toxicology 4:721-732)来检测。

“对象植物或植物细胞”是受到感兴趣的ABC转运体蛋白编码基因的影响而发生了遗传改变(如转化)的植物或植物细胞，或者是源自如此改变的植物或细胞并包含该改变的植物或植物细胞。“对照”或“对照植物”或“对照植物细胞”提供用于测量对象植物或植物细胞的表型变化的参考点。因此，取决于本发明的方法，感兴趣的ABC转运体蛋白编码基因的表达水平高于或低于对照植物中的表达水平。

对照植物或植物细胞可以包括例如：(a)野生型植物或细胞，即，基因型与用于在对象植物或细胞中产生遗传改变的起始材料相同；(b)基因型与起始材料相同、但用空构建体(即对感兴趣的性状没有已知的影响的构建体，例如包含标记基因的构建体)转化的植物或植物细胞；(c)对象植物或植物细胞的后代中为非转化分离子的植物或植物细胞；(d)在遗传上与对象植物或植物细胞相同、但未暴露在诱导感兴趣的基因表达的条件或刺激下的植物或植物细胞；或者(e)处于感兴趣的基因不表达的条件下的对象植物或植物细胞本身。

尽管本发明用术语转化的植物来描述，但应认识到，本发明的转化的生物体也包括植物细胞、植物原生质体、来自可再生植物的植物细胞组织培养物、植物愈伤组织、植物丛、以及在植物或植物部分中的完好植物细胞，例如胚、花粉、胚珠、种子、叶、花、枝条、果实、仁、穗、穗轴、壳、茎、根、根尖、花药等。谷物意在表示由商业种植者们为了物种的生长或再生产以外的目的而生产的成熟种子。再生植物的后代、变体和突变体也包括在本发明的范围内，前提是这些部分包含导入的多核苷酸。

为了下调感兴趣的ABC转运体蛋白编码基因的表达，可以构建反义构建体，该反义构建体与感兴趣的基因、尤其是编码感兴趣的ABC转运体蛋白的基因的序列的信使RNA(mRNA)的至少一部分互补。反义核苷酸设计成与相应的mRNA杂交。可以进行反义序列的改良，只要该序列与相应的mRNA杂交并干扰其表达。通过该方式，可以使用与欲沉默的相应序列具有70％、较佳为80％、更佳为85％、90％、95％或更高的序列相同性的反义构建体。另外，可以使用反义核苷酸的一部分来干扰靶基因的表达。

本发明的多核苷酸可用于从其它植物分离相应序列。通过该方式，可采用诸如PCR、杂交等方法，基于其相对于本文所示序列的序列同源性或相同性来鉴定这类序列。基于其相对于本文所示的完整序列或其变体和片段的序列相同性而分离的序列由本发明所涵盖。这类序列包括本公开序列的直系同源物的序列。"直系同源物"意在表示衍生自共同的祖先基因、并且作为物种形成的结果而存在于不同物种中的基因。存在于不同物种中的基因在其核苷酸序列和/或其编码的蛋白质序列共有至少75％、80％、85％、90％、91％、92％、93％、94％、95％、96％、97％、98％、99％或更高的序列相同性时，被认为是直系同源物。直系同源物的功能通常在物种间高度保守。因此，具有转录活性或增强子活性、并且与本文所述序列共有至少75％的序列相同性的分离多核苷酸或其变体或片段由本发明所涵盖。

变体序列可通过PCR来分离。设计PCR引物和PCR克隆的方法为本领域公知且公开于Sambrook等(1989)Molecular Cloning:A Laboratory Manual(《分子克隆：实验室手册》)(第二版，冷泉港实验室出版社，纽约普莱恩维尤)。另见Innis等编著，(1990)PCRProtocols:A Guide to Methods and Applications(《PCR方案：方法和应用指南》)(美国学术出版社，纽约)；Innis和Gelfand编著，(1995)PCR Strategies(《PCR策略》)(美国学术出版社，纽约)；以及Innis和Gelfand编著，(1999)PCR Methods Manual(《PCR方法手册》)(美国学术出版社，纽约)。

变体序列也可以通过现有的已测序基因组数据库的分析来鉴定。通过该方式，可鉴定编码ABC转运体蛋白的相应序列并用于本发明的方法。变体序列保留ABC转运体蛋白的生物活性(即ATP的水解和无机离子的转运)。本发明显示，出乎意料的是，ABC转运体蛋白过表达的某些新型表达策略可导致生物质和种子产量增加。

表达盒在转录物的5'-3'方向上包含：转录和翻译起始区、编码本发明的ABC转运体蛋白的多核苷酸、以及在植物中起作用的转录和翻译终止区(即终止区)。

多种启动子可用于实施本发明。编码本发明的ABC转运体蛋白的多核苷酸可以自具有组成型表达谱的启动子表达。组成型启动子包括CaMV 35S启动子(Odell等(1985)Nature 313:810-812)；水稻肌动蛋白(McElroy等(1990)Plant Cell 2:163-171)；泛素(Christensen等(1989)Plant Mol.Biol.12:619-632和Christensen等(1992)PlantMol.Biol.18:675-689)；pEMU(Last等(1991)Theor.Appl.Genet.81:581-588)；MAS(Velten等(1984)EMBO J.3:2723-2730)；ALS启动子(美国专利第5,659,026号)等。

编码本发明的ABC转运体蛋白的本发明的多核苷酸可以由组织偏好性启动子表达。组织偏好性启动子包括Yamamoto等(1997)Plant J.12(2):255-265；Kawamata等(1997)Plant Cell Physiol.38(7):792-803；Hansen等(1997)Mol.Gen Genet.254(3):337-343；Russell等(1997)Transgenic Res.6(2):157-168；Rinehart等(1996)Plant Physiol.112(3):1331-1341；Van Camp等(1996)Plant Physiol.112(2):525-535；Canevascini等(1996)Plant Physiol.112(2):513-524；Yamamoto等(1994)Plant Cell Physiol.35(5):773-778；Lam(1994)Results Probl.Cell Differ.20:181-196；Orozco等(1993)Plant MolBiol.23(6):1129-1138；Matsuoka等(1993)Proc Natl.Acad.Sci.USA90(20):9586-9590；以及Guevara-Garcia等(1993)Plant J.4(3):495-505。叶偏好性启动子也是本领域已知的。参见例如Yamamoto等(1997)Plant J.12(2):255-265；Kwon等(1994)PlantPhysiol.105:357-67；Yamamoto等(1994)Plant Cell Physiol.35(5):773-778；Gotor等(1993)Plant J.3:509-18；Orozco等(1993)Plant Mol.Biol.23(6):1129-1138；以及Matsuoka等(1993)Proc.Natl.Acad.Sci.USA 90(20):9586-9590。

对于编码ABC转运体蛋白的多核苷酸的表达而言，发育调控启动子可能是理想的。这类启动子可以在特定的发育阶段显示出表达峰值。这类启动子已在本领域中描述，例如US 62/029,068；Gan和Amasino(1995)Science 270:1986-1988；Rinehart等(1996)PlantPhysiol 112:1331-1341；Gray-Mitsumune等(1999)Plant Mol Biol 39:657-669；Beaudoin和Rothstein(1997)Plant Mol Biol 33:835-846；Genschik等(1994)Gene 148:195-202等。

对于编码ABC转运体蛋白的多核苷酸的表达而言，在施加特定的生物胁迫和/或非生物胁迫后被诱导的启动子可能是理想的。这类启动子已在本领域中描述，例如Yi等(2010)Planta 232:743-754；Yamaguchi-Shinozaki和Shinozaki(1993)Mol Gen Genet236:331-340；U.S.Patent No.7,674,952；Rerksiri等(2013)Sci World J 2013:ArticleID 397401；Khurana等(2013)PLoS One 8:e54418；Tao等(2015)Plant Mol Biol Rep 33:200-208等。

对于编码ABC转运体蛋白的多核苷酸的表达而言，细胞偏好性启动子可能是理想的。这类启动子可以在特定的细胞种类、例如叶肉细胞或维管束鞘细胞中偏好性地驱动下游基因的表达。这类细胞偏好性启动子已在本领域中描述，例如Viret等(1994)Proc NatlAcad USA 91:8577-8581；美国专利第8,455,718号；美国专利第7,642,347号；Sattarzadeh等(2010)Plant Biotechnol J 8:112-125；Engelmann等(2008)Plant Physiol 146:1773-1785；Matsuoka等(1994)Plant J 6:311-319等。

应认识到，相对于感兴趣的一个或多个基因的组成型表达，特定的非组成型表达谱可提供改善的植物表型。例如，许多植物基因是通过光照条件、施加特定胁迫、昼夜循环或植物的发育阶段来调节的。这些表达谱对于植物体中的基因或基因产物的功能而言可能是很重要的。可以用于提供所需表达谱的一种策略是使用含有顺式调节元件的合成启动子，其在植物中的所需时间和位置驱动所需的表达水平。可用于改变植物体中的基因表达的顺式调节元件已在科技文献(Vandepoele等(2009)Plant Physiol 150:535-546；Rushton等(2002)Plant Cell 14:749-762)中描述。顺式调节元件也可以用于改变启动子表达谱，如Venter(2007)Trends Plant Sci 12:118-124中所述。

植物终止子是本领域已知的，包括可获自根癌农杆菌(A.tumefaciens)的Ti质粒的植物终止子，例如章鱼碱合酶和胭脂碱合酶终止区。另见Guerineau等(1991)Mol.Gen.Genet.262:141-144；Proudfoot(1991)Cell 64:671-674；Sanfacon等(1991)Genes Dev.5:141-149；Mogen等(1990)Plant Cell 2:1261-1272；Munroe等(1990)Gene91:151-158；Ballas等(1989)Nucleic Acids Res.17:7891-7903；以及Joshi等(1987)Nucleic Acids Res.15:9627-9639。

如所示，编码本发明的ABC转运体蛋白的核苷酸可以用于表达盒中来转化感兴趣的植物。转化方案以及将多肽或多核苷酸序列导入植物中的方案可以变化，取决于作为转化靶标的植物或植物细胞的种类，即单子叶植物或双子叶植物。术语“进行转化”或“转化”是指用于将多肽或多核苷酸导入植物细胞中的任何方法。将多肽或多核苷酸导入植物细胞中的合适方法包括微注射(Crossway等(1986)Biotechniques 4:320-334)、电穿孔(Riggs等(1986)Proc.Natl.Acad.Sci.USA 83:5602-5606)、农杆菌介导的转化(美国专利第5,563,055号和美国专利第5,981,840号)、直接基因转移(Paszkowski等(1984)EMBO J.3:2717-2722)、以及射弹粒子加速(参见例如美国专利第4,945,050号；美国专利第5,879,918号；美国专利第5,886,244号；以及美国专利第5,932,782号；Tomes等(1995)在Plant Cell,Tissue,and Organ Culture:Fundamental Methods(《植物细胞、组织和器官培养：基础方法》)中，Gamborg和Phillips编著(施普林格出版社(Springer-Verlag)，柏林)；McCabe等(1988)Biotechnology 6:923-926)；以及Lec1转化(WO 00/28058)。另见Weissinger等(1988)Ann.Rev.Genet.22:421-477；Sanford等(1987)Particulate Science andTechnology 5:27-37(洋葱)；Christou等(1988)Plant Physiol.87:671-674(大豆)；McCabe等(1988)Bio/Technology 6:923-926(大豆)；Finer和McMullen(1991)In VitroCell Dev.Biol.27P:175-182(大豆)；Singh等(1998)Theor.Appl.Genet.96:319-324(大豆)；Datta等(1990)Biotechnology 8:736-740(水稻)；Klein等(1988)Proc.Natl.Acad.Sci.USA 85:4305-4309(玉米)；Klein等(1988)Biotechnology 6:559-563(玉米)；美国专利第5,240,855号；美国专利第5,322,783号；以及美国专利第5,324,646号；Klein等(1988)Plant Physiol.91:440-444(玉米)；Fromm等(1990)Biotechnology 8:833-839(玉米)；Hooykaas-Van Slogteren等(1984)Nature(伦敦)311:763-764；美国专利第5,736,369号(谷类)；Bytebier等(1987)Proc.Natl.Acad.Sci.USA 84:5345-5349(百合科)；De Wet等(1985)在The Experimental Manipulation of Ovule Tissues(《胚珠组织的实验操作》)中，Chapman等编著(朗文出版社(Longman)，纽约)，第197-209页(花粉)；Kaeppler等(1990)Plant Cell Reports 9:415-418和Kaeppler等(1992)Theor.Appl.Genet.84:560-566(触须介导的转化)；D'Halluin等(1992)Plant Cell 4:1495-1505(电穿孔)；Li等(1993)Plant Cell Reports 12:250-255及Christou和Ford(1995)Annals of Botany 75:407-413(水稻)；Osjoda等(1996)Nature Biotechnology14:745-750(玉米，通过根癌农杆菌(Agrobacterium tumefaciens))；所有这些文献通过引用纳入本文。"稳定转化"意在表示导入植物中的核苷酸构建体整合至植物的基因组中，并能够由其后代遗传。

经转化的细胞可根据常规方式生长成植物。参见例如McCormick等(1986)PlantCell Reports 5:81-84。通过该方式，本发明提供转化的种子(也称为“转基因种子”)，在其基因组中稳定地引入了本发明的多核苷酸，例如本发明的表达盒。

本发明可以用于转化任何植物种类，包括但不限于单子叶植物和双子叶植物。感兴趣的植物种类的例子包括但不限于：玉米(Zea mays)、芸薹属(例如甘蓝型油菜(B.napus)、白菜型油菜(B.rapa)、芥菜型油菜(B.juncea))、尤其是用作菜籽油来源的油菜种类、紫花苜蓿(Medicago sativa)、水稻(Oryza sativa)、黑麦(Secale cereale)、高粱(双色高粱(Sorghum bicolor)、红棕色高粱(Sorghum vulgare))、粟(例如珍珠粟(Pennisetum glaucum)、黍(Panicum miliaceum)、小米(Setaria italica)、龙爪稷(Eleusine coracana))、向日葵(Helianthus annuus)、红花(Carthamus tinctorius)、小麦(Triticum aestivum)、大豆(Glycine max)、烟草(Nicotiana tabacum)、马铃薯(Solanum tuberosum)、花生(Arachis hypogaea)、棉(海岛棉(Gossypium barbadense)、陆地棉(Gossypium hirsutum))、甘薯(Ipomoea batatus)、木薯(Manihot esculenta)、咖啡(Coffea spp.)、椰子(Cocos nucifera)、菠萝(Ananas comosus)、柑橘树(Citrus spp.)、可可(Theobroma cacao)、茶(Camellia sinensis)、橡胶(Musa spp.)、鳄梨(Perseaamericana)、无花果(Ficus casica)、番石榴(Psidium guajava)、芒果(Mangiferaindica)、油橄榄(Olea europaea)、木瓜(Carica papaya)、腰果(Anacardiumoccidentale)、澳洲坚果(Macadamia integrifolia)、扁桃(Prunus amygdalus)、甜菜(Beta vulgaris)、甘蔗(Saccharum spp.)、油棕(Elaeis guineensis)、杨(Populusspp.)、桉(Eucalyptus spp.)、燕麦(Avena sativa)、大麦(Hordeum vulgare)、蔬菜、观赏植物和针叶树。

在一个实施方式中，使用含有在植物细胞中可操作的启动子的构建体来转化植物细胞，该启动子与编码本发明的ABC转运体蛋白的编码序列操作性连接。令转化的植物细胞再生，以生产转化的植物。这些用含有驱动本发明的ABC转运体蛋白编码多核苷酸表达的功能性启动子的构建体转化的植物被证明具有增加的植物产量，即增加的地上生物质和/或增加的可收获生物质和/或增加的种子产量。

现已证明，ABC转运体的上调可增加植物产量，也可采用用于增加感兴趣的植物中的内源性ABC转运体序列表达的其它方法。植物基因组中存在的ABC转运体基因的表达可以通过在植物基因组中存在的ABC转运体基因的上游插入转录增强子来改变。该策略将允许ABC转运体基因的表达保持其正常发育状况，而又显示出升高的转录物水平。该策略将通过采用针对感兴趣的基因组序列设计的大范围核酸酶在感兴趣的ABC转运体基因的上游插入增强子元件而实现。或者，采用与针对感兴趣的基因组序列设计的导引RNA(gRNA)偶联的Cas9核酸内切酶、或与针对感兴趣的基因组序列设计的gRNA偶联的Cpf1核酸内切酶、或与针对感兴趣的基因组序列设计的gRNA偶联的Csm1核酸内切酶，在感兴趣的ABC转运体基因的上游插入增强子元件。或者，将与转录增强子元件融合的失活核酸内切酶(例如失活的Cas9、Cpf1或Csm1核酸内切酶)靶向感兴趣的ABC转运体基因的转录起始位点附近的基因组位置，从而调节所述感兴趣的ABC转运体基因的表达(Piatek等(2015)Plant Biotechnol J13:578-589)。

ABC转运体蛋白编码基因表达的调节可以通过使用精确基因组编辑技术调节内源性序列的表达来实现。通过该方式，采用本领域已知的方法将核酸序列插入至编码ABC转运体的天然植物序列附近。这类方法包括但不限于：针对感兴趣的植物基因组序列设计的大范围核酸酶(D’Halluin等(2013)Plant Biotechnol J 11:933-941)；CRISPR-Cas9、CRISPR-Cpf1、TALENs及其它用于精确编辑基因组的技术(Feng等(2013)Cell Research23:1229-1232，Podevin等(2013)Trends Biotechnology 31:375-383，Wei等(2013)J GenGenomics 40:281-289，Zhang等(2013)WO 2013/026740，Zetsche等(2015)Cell 163:759-771，美国临时专利申请62/295,325)；格氏嗜盐碱杆菌Argonaute蛋白介导的DNA插入(Gao等(2016)Nat Biotechnol doi:10.1038/nbt.3547)；Cre-lox位点特异性重组(Dale等(1995)Plant J 7:649-659；Lyznik等(2007)Transgenic Plant J 1:1-9；FLP-FRT重组(Li等(2009)Plant Physiol 151:1087-1095)；Bxb1介导的整合(Yau等(2011)Plant J 701:147-166)；锌指蛋白介导的整合(Wright等(2005)Plant J 44:693-705)；Cai等(2009)Plant Mol Biol 69:699-709)；以及同源重组(Lieberman-Lazarovich和Levy(2011)Methods Mol Biol 701:51-65；Puchta(2002)Plant Mol Biol 48:173-182)。所述核酸序列的插入将用于实现过表达、减少表达和/或改变ABC转运体基因表达谱的所需效果。

增强子包括在插入植物的基因组中时能够增强基因表达的任何分子。因此，增强子可被插入感兴趣的ABC转运体序列的基因组上游或下游区域以增强表达。增强子可以是顺式作用的，并且相对于欲增强其表达的基因可位于基因组中的任何位置。例如，增强子可以位于其增强表达的编码序列的约1Mbp、约100kbp、约50kbp、约30kbp、约20kbp、约10kbp、约5kbp、约3kbp或约1kbp范围内。增强子也可以位于其增强表达的基因的约1500bp范围内，或者可以直接毗邻其增强表达的基因的内含子或位于其增强表达的基因的内含子内。用于调节编码本发明的ABC转运体蛋白或同源物的内源基因表达的增强子包括经典的增强子元件，如CaMV 35S增强子元件、巨细胞病毒(CMV)早期启动子增强子元件、SV40增强子元件、以及增强基因表达的内含子介导增强子元件如玉米shrunken-1增强子元件(Clancy和Hannah(2002)Plant Physiol.130(2):918-29)。可以导入植物基因组中来调节表达的增强子的例子还包括PetE增强子(Chua等(2003)Plant Cell 15:11468-1479)或水稻α-淀粉酶增强子(Chen等(2002)J.Biol.Chem.277:13641-13649)或本领域已知的任何增强子(Chudalayandi(2011)Methods Mol.Biol.701:285-300)。在一些实施方式中，本发明包括亚结构域、片段或重复增强子元件(Benfrey等(1990)EMBO J 9:1677-1684)。

ABC转运体基因表达的改变也可以通过以不会改变DNA序列的方式修饰DNA来实现。这类变化可以包括修饰感兴趣的ABC转运体基因和/或围绕ABC转运体基因的DNA的染色质成分或结构。众所周知，这类染色质成分或结构的变化能影响基因转录(Hirschhorn等(1992)Genes and Dev 6:2288-2298；Narlikar等(2002)Cell 108:475-487)。这类变化也可以包括改变感兴趣的ABC转运体基因和/或围绕感兴趣的ABC转运体基因的DNA的甲基化状态。众所周知，这类DNA甲基化的变化能改变转录(Hsieh(1994)Mol Cell Biol 14:5487-5494)。已证明靶向表观基因组编辑以可预期方式影响基因的转录(Hilton等(2015)33:510-517)。对本领域技术人员来说显而易见的是，为了实现改变ABC转运体基因表达谱的所需效果，可以对调节感兴趣的ABC转运体基因转录的DNA施加其它类似的改变(统称为“表观遗传学改变”)。

ABC转运体基因表达的改变也可以通过使用转座因子技术改变基因表达来实现。应理解，转座因子可改变邻近DNA的表达(McGinnis等(1983)Cell 34:75-84)。编码ABC转运体的基因表达的改变可以通过在感兴趣的ABC转运体基因的上游插入转座因子、导致所述基因的表达发生改变来实现。

ABC转运体基因表达的改变也可以通过对调节感兴趣的ABC转运体基因表达的一个或多个转录因子进行表达来实现。应理解，转录因子表达的改变可进而改变所述转录因子的靶基因的表达(Hiratsu等(2003)Plant J 34:733-739)。ABC转运体基因表达的改变也可以通过改变已知与感兴趣的ABC转运体基因相互作用的转录因子的表达来实现(例如OCL1；Javelle等(2010)Plant Physiol 154:273-286)。

ABC转运体基因表达的改变也可以通过在感兴趣的植物种类中编码天然ABC转运体的开放阅读框的上游插入启动子来实现。这将通过采用针对感兴趣的基因组序列设计的大范围核酸酶、在编码ABC转运体蛋白的开放阅读框的上游插入感兴趣的启动子来实现。该策略是众所周知的，并且先前已经证明在棉花基因组中的预定位置插入转基因(D’Halluin等(2013)Plant Biotechnol J 11:933-941)。对本领域技术人员来说显而易见的是，可以采用其它技术，通过在预定的基因组基因座处引起双链断裂并提供适合于插入的DNA模板，来实现在所述预定的基因组基因座处插入遗传元件的类似结果(例如CRISPR-Cas9、CRISPR-cpf1、CRISPR-Csm1、TALENs及其它用于精确编辑基因组的技术)。

通过说明的方式，而非限制性方式提供以下实施例。本说明书中涉及的所有出版物和专利申请表明本发明涉及领域技术人员的水平。所有出版物和专利申请通过引用纳入本文，就好像将各篇单独的出版物或专利申请具体和单独地通过引用纳入本文那样。

虽然出于方便理解的目的，通过阐述和举例的方式详细描述了上述发明，但可明显看出，某些改变和修改应属于所附权利要求书的范围。

实验部分

实施例1–ABC转运体植物转化载体的构建

合成编码玉米ABC转运体蛋白的开放阅读框。该开放阅读框包含SEQ ID NO:1，编码SEQ ID NO:2的蛋白质序列。在编码序列的5’和3’端包括合适的限制性位点，以允许该DNA被克隆至含有适合于控制基因表达的遗传元件的植物转化载体中。各植物转化构建体中，ABC转运体开放阅读框位于植物启动子和5’非翻译区(5’UTR)的下游、3’UTR的上游。表2汇总了构建成含有ABC转运体开放阅读框的植物转化构建体。

表2：ABC转运体植物转化构建体

除了表2所列的单基因ABC转运体植物转化构建体外，也构建了含有ABC转运体基因盒和连接的第二盒的多基因植物转化构建体。表3汇总了多基因ABC转运体植物转化构建体。

表3：ABC转运体多基因植物转化构建体

除了表2和3所示的基因盒外，表2和3所列的各植物转化构建体还含有选择性标记盒，其适合于选择转化的植物细胞并在导入植物转化载体后使植物再生，如下所述。各转化载体构建在含有适合于在大肠杆菌和根癌农杆菌中保持质粒的序列的质粒中。验证表2和3所列的植物转化构建体含有所需序列，然后将它们转化至植物转化用的根癌农杆菌中。

实施例2–狗尾草(Setaria viridis)的转化

根据以上所述的方法(PCT/US2015/43989，通过引用纳入本文)，用携带ABC转运体植物转化载体的根癌农杆菌细胞转化狗尾草细胞。用相关植物转化载体转化狗尾草细胞，并再生狗尾草植物，然后进行PCR分析以确认狗尾草基因组中存在感兴趣的基因。表4汇总了用于转化狗尾草的转化构建体，以及经PCR验证的用各构建体转化而得的转基因植物的数量。

表4：用ABC转运体植物转化载体转化狗尾草的汇总

<u>构建体</u>	<u>#植株</u>
		131223	25
131220	31

实施例3–玉米(Zea mays)的转化

用携带132141构建体(表2)的根癌农杆菌细胞转化适合于在组织培养基中再生的玉米(Zea mays cv.B104)细胞。转化玉米细胞，并再生玉米植物，然后进行PCR分析以确认玉米基因组中存在感兴趣的基因。生产了45个转化的植株，并通过分子试验确认包含感兴趣的基因。

实施例4–水稻(Oryza sativa)的转化

用携带ABC转运体植物转化载体的根癌农杆菌细胞转化适合于在组织培养基中再生的水稻(Oryza sativa cv.Kitaake)细胞。用相关植物转化载体转化水稻细胞，并再生水稻植物，然后进行PCR分析以确认水稻基因组中存在感兴趣的基因。

实施例5–转基因狗尾草的表征

用ABC转运体植物转化载体转化狗尾草并再生，然后培养T0代植物至成熟，以生产T1代种子。携带感兴趣的ABC转运体基因盒的T1代狗尾草在温室中生长，以评估ABC转运体基因表达对植物生长、最终地上生物质和种子产量的影响。采用随机区组设计，用野生型狗尾草边行来消除分析中的边缘效应。空分离子植物在相同的环境条件下生长在转基因狗尾草植物边上。表5汇总了用携带ABC转运体基因盒的T1代狗尾草植物进行的实验中得到的生物质和种子产量测定的结果，作为转化的结果(实验S60和S68)。实验U16显示用通过所示T1植株的自花授粉生产的T2代狗尾草进行的实验的生物质和种子产量的结果。该表所示为用于转化的构建体，如表2所述，然后是从中收获了T1种子的T0植株数量。

表5：采用T1代植物的狗尾草温室观察的汇总

表5中，地上生物质的干重以克数示于DW栏中。类似地，收获种子的干重以克数示于种子产量栏中。DW变化和种子变化栏分别所示为地上生物质和种子产量相对于来自131220构建体的空分离子的变化百分数。如该表所示，实验S68中经测试的所有五个131220植株相对于空分离子都具有增加的地上生物质积累，经测试的五个131220植株中有三个相对于空分离子具有增加的种子产量。实验S60中，经测试的五个131223植株中有一个相对于空分离子对照产生了增加的生物质和种子产量。实验U16中，测试了通过自花授粉生产的T2代植株的生物质产量和种子产量。这些测试中，植株14A相对于空分离子再一次显示出生物质产量的增加和种子产量的减少；植株19B显示出增加的生物质产量和种子产量；植株26相对于空分离子显示出增加的种子产量。

实施例6–转基因玉米的表征

用感兴趣的ABC转运体植物转化载体转化、并确认含有感兴趣的基因的T0代玉米植物在温室中生长至成熟。当T0植物进入繁殖期时，用合适的近交玉米品系对它们授粉，以产生杂交玉米种子。或者，除了T0代转基因玉米植物的授粉外，还用来自T0的花粉对一个或多个近交玉米品系授粉，以产生杂交玉米种子。将通过这些授粉而得的F1代杂交种子种植在田地里，设置为两行或四行的小块土地，用标准农艺实践培养。检测植物的基因型，以确定哪些植物含有、哪些植物不含有ABC转运体基因盒以及包含在ABC转运体植物转化载体中的其它任何相关基因盒(例如选择性标记基因盒)。玉米植物成熟后，收获种子。收集来自含有ABC转运体基因盒的植物的种子，同样也收集来自缺少ABC转运体基因盒的空分离子植物的种子。对种子称重，并计算含有ABC转运体基因盒的植物以及缺少ABC转运体基因盒的空分离子植物的种子产量。进行合适的统计学分析来确定含有ABC转运体基因盒的植物是否具有比缺少ABC转运体基因盒的植物更高的产量。

或者，用感兴趣的ABC转运体植物转化载体转化、并确认含有感兴趣的基因的T0代玉米植物在温室中生长至成熟，然后自花授粉。将所得的T1种子种植在温室中，并培养T1植物。检测T1植物的基因型，以鉴定纯合子、杂合子和空分离子植物。用来自纯合子T1植物的花粉对一个或多个近交玉米品系授粉，以产生杂交玉米种子。也用来自空分离子植物的花粉对一个或多个近交玉米品系授粉，以产生杂交玉米种子。将所得的杂交种子种植在田地里，设置为两行或四行的小块土地，用标准农艺实践培养。玉米植物成熟后，收获种子。收集来自含有ABC转运体基因盒的植物的种子，同样也收集来自缺少ABC转运体基因盒的空分离子植物的种子。对种子称重，并计算含有ABC转运体基因盒的植物以及缺少ABC转运体基因盒的空分离子植物的种子产量。进行合适的统计学分析来确定含有ABC转运体基因盒的植物是否具有比缺少ABC转运体基因盒的植物更高的产量。

实施例7–转基因水稻的表征

用感兴趣的ABC转运体植物转化载体转化、并确认含有感兴趣的基因的T0代水稻植物在温室中生长至成熟，然后自花授粉。将所得的T1种子种植在温室中，并培养T1植物。检测T1植物的基因型，以鉴定纯合子、杂合子和空分离子植物。让来自各组的植物生长至成熟，并自花授粉以产生T2种子。将由该自花授粉得到的T2种子收获并称重，计算来自纯合子、杂合子和空分离子植物的种子产量。进行合适的统计学分析来确定含有ABC转运体基因盒的植物是否具有比缺少ABC转运体基因盒的植物更高的产量。

由T0代植物的自花授粉得到的种子长成的T1代植物、或者由纯合子T1代植物的自花授粉得到的种子长成的T2代植物在田地里生长。对于T2代植物，对空分离子T1代植物也进行自花授粉，以产生T2代空植物作为阴性对照。用标准农艺实践培养这些植物，令其达到成熟。达到成熟后，让这些植物自花授粉。将由这些自花授粉得到的种子收获并称重，计算来自纯合子、杂合子和空分离子植物的种子产量。进行合适的统计学分析来确定含有ABC转运体基因盒的植物是否具有比缺少ABC转运体基因盒的植物更高的产量。

序列表

<110> 本森希尔股份有限公司（Benson Hill Biosystems, Inc.）

<120> 通过使用ABC转运体序列来增加植物生长和产量

<130> BHP014P

<160> 103

<170> PatentIn version 3.5

<210> 1

<211> 888

<212> DNA

<213> 玉米(Zea mays)

<220>

<221> misc_feature

<222> (1)..(888)

<223> ABC转运体

<400> 1

atggccggcg aagggaatgg ggatgaaggg tggaggcgga gcggcatcga ggtcagcgcc 60

ctgcagttcg gctacgacgg gcagccgccg ctcttcgcgc gcttcaacct ctgcgtcgca 120

cccggctccc gctgcctcct cgtcggcgcc aacggatcag gcaagaccac actcttgaag 180

attcttgcgg gaaagcatat ggttggagga agagatgtgg tccgtgtcct caatggttcc 240

gcttttcatg atacacagct agtgtgcaat ggtgaccttt cgtacttggg tggttcttgg 300

agccgtacta ttggttcagc tggggatgtt ccactgcaag gcgacttctc tgctgagcac 360

atgatttttg gagttgatgg ggttgatcct gtcaggcgag agaagctggt tgatctgcta 420

gacattgatc tgcagtggcg catgcataaa gtttcagatg ggcagcgccg cagggtgcaa 480

atctgcatgg gtcttcttca tccatacaag gtgcttttgc tcgatgagat cacggttgat 540

ctggacgtgg tgaccaggat ggacctgctt ggtttcttca aggaagagtg cgagcagagg 600

gaagctacca tcgtgtacgc cacccatata tttgacggac tcgagacgtg ggctaccgac 660

tttgcgtaca tccaagaagg cgagctgaga aggtccggga gatactccga catcgaggag 720

ctaaggagcg ccaagaactt gctgtcggta gtcgagtcgt ggctgaggtc agagaccaaa 780

cttccgaaga aggaaccccc accacgtcct gagacccagg ccaggcgttc ctcgccgttc 840

gatgcttctc ctttccgttc gtcacgccac atggcctact accgttga 888

<210> 2

<211> 295

<212> PRT

<213> 玉米(Zea mays)

<220>

<221> MISC_FEATURE

<222> (1)..(295)

<223> ABC转运体

<400> 2

Met Ala Gly Glu Gly Asn Gly Asp Glu Gly Trp Arg Arg Ser Gly Ile

1 5 10 15

Glu Val Ser Ala Leu Gln Phe Gly Tyr Asp Gly Gln Pro Pro Leu Phe

20 25 30

Ala Arg Phe Asn Leu Cys Val Ala Pro Gly Ser Arg Cys Leu Leu Val

35 40 45

Gly Ala Asn Gly Ser Gly Lys Thr Thr Leu Leu Lys Ile Leu Ala Gly

50 55 60

Lys His Met Val Gly Gly Arg Asp Val Val Arg Val Leu Asn Gly Ser

65 70 75 80

Ala Phe His Asp Thr Gln Leu Val Cys Asn Gly Asp Leu Ser Tyr Leu

85 90 95

Gly Gly Ser Trp Ser Arg Thr Ile Gly Ser Ala Gly Asp Val Pro Leu

100 105 110

Gln Gly Asp Phe Ser Ala Glu His Met Ile Phe Gly Val Asp Gly Val

115 120 125

Asp Pro Val Arg Arg Glu Lys Leu Val Asp Leu Leu Asp Ile Asp Leu

130 135 140

Gln Trp Arg Met His Lys Val Ser Asp Gly Gln Arg Arg Arg Val Gln

145 150 155 160

Ile Cys Met Gly Leu Leu His Pro Tyr Lys Val Leu Leu Leu Asp Glu

165 170 175

Ile Thr Val Asp Leu Asp Val Val Thr Arg Met Asp Leu Leu Gly Phe

180 185 190

Phe Lys Glu Glu Cys Glu Gln Arg Glu Ala Thr Ile Val Tyr Ala Thr

195 200 205

His Ile Phe Asp Gly Leu Glu Thr Trp Ala Thr Asp Phe Ala Tyr Ile

210 215 220

Gln Glu Gly Glu Leu Arg Arg Ser Gly Arg Tyr Ser Asp Ile Glu Glu

225 230 235 240

Leu Arg Ser Ala Lys Asn Leu Leu Ser Val Val Glu Ser Trp Leu Arg

245 250 255

Ser Glu Thr Lys Leu Pro Lys Lys Glu Pro Pro Pro Arg Pro Glu Thr

260 265 270

Gln Ala Arg Arg Ser Ser Pro Phe Asp Ala Ser Pro Phe Arg Ser Ser

275 280 285

Arg His Met Ala Tyr Tyr Arg

290 295

<210> 3

<211> 1416

<212> DNA

<213> 水稻(Oryza sativa)

<220>

<221> 启动子

<222> (1)..(1416)

<223> OsCA启动子

<400> 3

atcttggcta tttcccatgg cttctccgct ctactcttgt ccctgtttgg atgacgccgt 60

ccagaccaag acatcaaaac ggggcgacac tgtgaggtta cggtgtcgcc attcgccaca 120

atgtgctcac catgtcacct tgtcgacatt tcgccgcata caagtcgctg tgcgaccgcc 180

aggtgggccc cactgtgagc agcacgagtg tggcgcgtat ataaatttgt cggatggaga 240

ggcagctgaa ggtttttcgc catggcaact gcgtccttcg acatctgcgc gaaggttggg 300

ctcggaattt cgacaggata ctcacagcga aatcaatact ctgctatggc aaatggtacg 360

cactcgtttc gattgttctg ccttcttctg ttttattttt tttcccgtat gtagctgtag 420

ctgctgataa acgtgccatg tattccatct cgttcttgca agcagtttct tagatgagtt 480

aaaatatgta ttccatgtca actgttcact ttagttaggt agaactttct tacattatga 540

ttagttatct acttactctc tctgtccgat aataattatc gcattgattt tttttataat 600

gtttgatcat tcgtcttatt aaaaaaaatt atagaattat tttttttatt ttgtttgtga 660

cttgctttat tatcaaaaaa ataatttaaa tatggcatat ctttttttat atttacaata 720

atttttcaaa aaagatgaat ggtcaaacgt tacacgaaaa aatcaaagcg accactattt 780

tggaatggaa gtagtacctg tagagaaaaa ttaaatatta gttcttaagt gagtgtggac 840

cgaaaaaatt ccttatttat cataaacacg ttttccaaac ttttaaatga tatgtttttt 900

taaatatata aatgaacatg ttctttcaaa aatcaaataa atcacttttt caagtttgta 960

ctgattaata ctagactaat catttgctaa ttgtttatat tgttttactt gccatcataa 1020

ctcatgccaa attgcttttc caaacccacc attagccgct gtggcaagct cagttgctag 1080

cttgaggagg actatacaaa gttgcacaca cgccatggta ctaacgagaa ctggaaaata 1140

tgttgactgg aaaaattgta tcagttcata ttagaaacaa attactgtca gaatgaggaa 1200

aaactcagtc catgccacta aaggcatcag atgcgaattg gcgctccttt ctcctttcaa 1260

ggagtaggca taaacatagg ctctgcagta gtttcatctg agacagtcgg cacgcggggg 1320

cgcgggcgtc tatttgttgc gcgcgcgggc gcggcggcga gacgcgtgtg tagctactgc 1380

tataaggagc gcgccgtgca ccgcctctca catcga 1416

<210> 4

<211> 315

<212> DNA

<213> 水稻(Oryza sativa)

<220>

<221> misc_feature

<222> (1)..(315)

<223> OsCA 3'UTR

<400> 4

gttcatccga ccgtccgtcc gttcagttcg tcagtttacg ccaacgcttt tgcataagta 60

ctacctgagg atatcgtccc cgatcatcga tgtgaacgcg tggagtacta ctacgtacgt 120

accggatggt tcgatatatg tgaatgctgt attaagtaat aacaagaaat atatctcctc 180

tactttttcc tgacgcggag ttgtactgcc tatgatgcat aatttgatcg cagtgtgatc 240

aaaagacatc agctataatg tcttaataat attattatga agagtttacc tttttactac 300

cttttactct ggtaa 315

<210> 5

<211> 1497

<212> DNA

<213> 水稻(Oryza sativa)

<220>

<221> 启动子

<222> (1)..(1497)

<223> OsRbcS启动子

<400> 5

gaaccatacg gaattgacgg accaattgtg catacggact tagctaaaat aattgttgat 60

ttttggcaat aagaaaagcg agtagcacat aaaatctaaa gtggatgagt aaagggacaa 120

aattttatac atgttcaggc cttctcgatg agaagtaata ctatactcct gttttgggga 180

ttatatttgt cagatgttgt atcaatctga cgatcgagtt atggttattg ttggcggctg 240

ttaaatatcg attttatgcc atcaatacct gtataattta tacagaaata ataaaacatt 300

caacatagtg gtaggcttta attctaacat attccataag tgttggtgta tatttggatg 360

caggtaataa accaccgaat taggaggaaa tctagactaa gttgaaggaa attttcatcc 420

atacaagtgt tgggcttttt aactccattt taacaccaaa atgcaagccc aaaaacctgc 480

gaaatggata aggcagactg agaaggaggc ccaggccaaa acttgggcca gttgggccaa 540

gccaggtttc ggccaaatcc tgatcatcgc tgttgatctc agggtttggc atggacgctc 600

ttgatttact cctgatggca gttgcagggc atttccgatc attcgcatgc tctacaacca 660

tcatacctac ttatttaagg agctctcatc ctcacttcat atcacacact ccaatcttga 720

gctgaattat aagaggctct attgtatttt attgtatact agaattaggg aaagattaag 780

gtcgtagaag aaatcggagg aattccggag ttatcggtga tccttttcta tttcttatac 840

tttgttattt gctttaatag aaatatcatt tcaagtaatt aagatttgtt tagtgagaac 900

tattattggc tagttcctaa ttagcgtatg agatcactgt tcactataat ccgttaaaat 960

atagtgattg ctttagtgag ttacaaacac tacagtagtt attgattgct taaacgtggt 1020

gtttagatag ttaatttcta gtggttgctg cgtatcccat agtacgttag aggcgggtgt 1080

agaggtggtg accgccctca agagcactta attcctcctt gtttgtgtac gtggtagagc 1140

gacatctggg aacagtgggt taccagtgcc tgaagtacca tgttaggatt aaaattgtaa 1200

cattgtttct cattagtaaa tcttctctac cctctaccca catttgcttt gtatccttgg 1260

tgaacctgaa gaggaactga acacacacgt tccatgagga agacactcag tactcaagcc 1320

ggaggcagca cactgcaact taagtttttc tatagctcct agcaagctag caatggctcc 1380

ctcggtgatg gcttcgtcgg ccacctccgt ggctcccttc caggggctca agtccactgc 1440

gggcctcccg gtgaaccgcc gctccagcag ctcgagcttt ggcaacgtca gcatcga 1497

<210> 6

<211> 1429

<212> DNA

<213> 水稻(Oryza sativa)

<220>

<221> misc_feature

<222> (1)..(1429)

<223> OsRbcS 3'UTR

<400> 6

gttcgcgctt tcgttccttc gtgcatgttc tttctttttc tttttttttt gtgtgtccgt 60

gttaagctgc acgtaattgt tctctcgcgc tccgacctgc cgttgttgca agagtactac 120

tacaactatc ggtctatcgt tcggtgacgg tgagacaggg cacgtgaatg caagatctcc 180

ggctatacac acgtactcat gtaatatgat gcctagagca tatctgaatc cgtcgacaat 240

gaaattttgg ttttgcaaaa tgctggtatt tgtttatcat cctggcacgt gatatttgcc 300

tagagcatct aaatcacttt tacgaaatgt gcgcgtcaac aaactgatac ggcccaaatg 360

ccagaaatta ccagcatata tagccatatc aacttttgat tcgtatatat gaaggttgat 420

ttagttagag aaattcggtt gtgagagaag gaggctagca aagattcggt tgatcaagct 480

gtaccgccag gccaggacgt gctgtgcgcg cggctgtgcc gcttgaccgc agaaccatac 540

ggaattgacg gaccaattgt gcatacggac ttagctaaaa taattgttga tttttggcaa 600

taagaaaagc gagtagcaca taaaatctaa agtggatgag taaagggaca aaattttata 660

catgttcagg ccttctcgat gagaagtaat actatactcc tgttttgggg attatatttg 720

tcagatgttg tatcaatctg acgatcgagt tatggttatt gttggcggct gttaaatatc 780

gattttatgc catcaatacc tgtataattt atacagaaat aataaaacat tcaacatagt 840

ggtaggcttt aattctaaca tattccataa gtgttggtgt atatttggat gcaggtaata 900

aaccaccgaa ttaggaggaa atctagacta agttgaagga aattttcatc catacaagtg 960

ttgggctttt taactccatt ttaacaccaa aatgcaagcc caaaaacctg cgaaatggat 1020

aaggcagact gagaaggagg cccaggccaa aacttgggcc agttgggcca agccaggttt 1080

cggccaaatc ctgatcatcg ctgttgatct cagggtttgg catggacgct cttgatttac 1140

tcctgatggc agttgcaggg catttccgat cattcgcatg ctctacaacc atcataccta 1200

cttatttaag gagctctcat cctcacttca tatcacacac tccaatcttg agctgaatta 1260

taagaggctc tattgtattt tattgtatac tagaattagg gaaagattaa ggtcgtagaa 1320

gaaatcggag gaattccgga gttatcggtg atccttttct atttcttata ctttgttatt 1380

tgctttaata gaaatatcat ttcaagtaat taagatttgt ttagtgaga 1429

<210> 7

<211> 525

<212> DNA

<213> 玉米(Zea mays)

<220>

<221> misc_feature

<222> (1)..(525)

<223> m型硫氧还蛋白

<400> 7

atggccctcg agacatgctt tagggcctgg gccctccacg ccgcgccagc cgggtccaag 60

gaccgcctcc tcgtgtgttc ctccgggggg aacctcgtcc tgccgtccaa gagggtcgcg 120

gccgcgccac tctccgtcgg cagggtcgcg acccgccgcg cccgccatgt gtgccagtcc 180

aaaaatgcgg tcgatgaagt gctcgtcgcg gatgaaaaaa actgggatgg catggtcatg 240

gcgtgtgaga ccccagtgct ggtcgaattc tgggccccat ggtgtgggcc gtgccgcatg 300

attgcgccgg tcatcgacga gctggcgaag gactatgcgg gcaaaattat gtgttgcaaa 360

gtcaatacag acgacagccc gaatgtcgcg tccacctacg gcattaggtc catcccaaca 420

gtgctcatct ttaaaggcgg cgagaagaaa gagagcgtca ttggcgcggt gccaaaaagc 480

acactcacaa ccctcatcga caaatatatc gggtccagct cctga 525

<210> 8

<211> 174

<212> PRT

<213> 玉米(Zea mays)

<220>

<221> MISC_FEATURE

<222> (1)..(174)

<223> m型硫氧还蛋白

<400> 8

Met Ala Leu Glu Thr Cys Phe Arg Ala Trp Ala Leu His Ala Ala Pro

1 5 10 15

Ala Gly Ser Lys Asp Arg Leu Leu Val Cys Ser Ser Gly Gly Asn Leu

20 25 30

Val Leu Pro Ser Lys Arg Val Ala Ala Ala Pro Leu Ser Val Gly Arg

35 40 45

Val Ala Thr Arg Arg Ala Arg His Val Cys Gln Ser Lys Asn Ala Val

50 55 60

Asp Glu Val Leu Val Ala Asp Glu Lys Asn Trp Asp Gly Met Val Met

65 70 75 80

Ala Cys Glu Thr Pro Val Leu Val Glu Phe Trp Ala Pro Trp Cys Gly

85 90 95

Pro Cys Arg Met Ile Ala Pro Val Ile Asp Glu Leu Ala Lys Asp Tyr

100 105 110

Ala Gly Lys Ile Met Cys Cys Lys Val Asn Thr Asp Asp Ser Pro Asn

115 120 125

Val Ala Ser Thr Tyr Gly Ile Arg Ser Ile Pro Thr Val Leu Ile Phe

130 135 140

Lys Gly Gly Glu Lys Lys Glu Ser Val Ile Gly Ala Val Pro Lys Ser

145 150 155 160

Thr Leu Thr Thr Leu Ile Asp Lys Tyr Ile Gly Ser Ser Ser

165 170

<210> 9

<211> 1647

<212> DNA

<213> 聚球蓝细菌(Synechococcus sp.)

<220>

<221> misc_feature

<222> (1)..(1647)

<223> RbcS-ictB

<220>

<221> 信号肽(sig_peptide)

<222> (1)..(339)

<223> 豌豆(P. sativum)RbcS信号肽

<400> 9

atggcttcta tgatttcttc ttctgctgtg acaacagtgt ctagggcttc taggggccag 60

tctgctgctg tggctccttt cggcggcctc aagtctatga caggcttccc tgtgaagaag 120

gtgaatacag atattacatc tattacatct aatggcggca gggtgaagtg catgcaggtg 180

tggcctccta ttggcaagaa gaagttcgag acactctctt acctccctcc tctcacaagg 240

gatatgacag tgtggcagac actcacattc gctcattacc agcctcagca gtggggccat 300

tcttctttcc tccataggct cttcggctct ctcagggctt ggagggcttc ttctcagctc 360

ctcgtgtggt ctgaggctct cggcggcttc ctcctcgctg tggtgtacgg ctctgctcct 420

ttcgtgcctt cttctgctct cggcctcggc ctcgctgcta ttgctgctta ctgggctctc 480

ctctctctca cagatattga tctcaggcag gctacaccta ttcattggct cgtgctcctc 540

tactggggcg tggatgctct cgctacaggc ctctctcctg tgagggctgc tgctctcgtg 600

ggcctcgcta agctcacact ctacctcctc gtgttcgctc tcgctgctag ggtgctcagg 660

aatcctaggc tcaggtctct cctcttctct gtggtggtga ttacatctct cttcgtgtct 720

gtgtacggcc tcaatcagtg gatttacggc gtggaggagc tcgctacatg ggtggatagg 780

aattctgtgg ctgatttcac atctagggtg tactcttacc tcggcaatcc taatctcctc 840

gctgcttacc tcgtgcctac aacagctttc tctgctgctg ctattggcgt gtggaggggc 900

tggctcccta agctcctcgc tattgctgct acaggcgctt cttctctctg cctcattctc 960

acatactcta ggggcggctg gctcggcttc gtggctatga ttttcgtgtg ggctctcctc 1020

ggcctctact ggttccagcc taggctccct gctccttgga ggaggtggct cttccctgtg 1080

gtgctcggcg gcctcgtggc tgtgctcctc gtggctgtgc tcggcctcga gcctctcagg 1140

gtgagggtgc tctctatttt cgtgggcagg gaggattctt ctaataattt caggattaat 1200

gtgtggctcg ctgtgctcca gatgattcag gataggcctt ggctcggcat tggccctggc 1260

aatacagctt tcaatctcgt gtaccctctc taccagcagg ctaggttcac agctctctct 1320

gcttactctg tgcctctcga ggtggctgtg gagggcggcc tcctcggcct cacagctttc 1380

gcttggctcc tcctcgtgac agctgtgaca gctgtgcgcc aggtgtctag gctcaggagg 1440

gataggaatc ctcaggcttt ctggctcatg gcttctctcg ctggcctcgc tggcatgctc 1500

ggccatggcc tcttcgatac agtgctctac aggcctgagg cttctacact ctggtggctc 1560

tgcattggcg ctattgcttc tttctggcag cctcagcctt ctaagcagct ccctcctgag 1620

gctgagcatt ctgatgagaa gatgtga 1647

<210> 10

<211> 580

<212> PRT

<213> 聚球蓝细菌(Synechococcus sp.)

<220>

<221> MISC_FEATURE

<222> (1)..(580)

<223> RbcS-ictB

<220>

<221> MISC_FEATURE

<222> (1)..(113)

<223> 豌豆(P. sativum)RbcS信号肽

<400> 10

Met Pro Ser Val Trp Gly Ser Leu Leu Gln Asp Asp Pro Ser Lys Gln

1 5 10 15

Ala Ser Ser Glu Tyr Ile His Thr Arg Gln Pro Gly Ser Arg Leu Asn

20 25 30

Met Ala Ser Met Ile Ser Ser Ser Ala Val Thr Thr Val Ser Arg Ala

35 40 45

Ser Arg Gly Gln Ser Ala Ala Val Ala Pro Phe Gly Gly Leu Lys Ser

50 55 60

Met Thr Gly Phe Pro Val Lys Lys Val Asn Thr Asp Ile Thr Ser Ile

65 70 75 80

Thr Ser Asn Gly Gly Arg Val Lys Cys Met Gln Val Trp Pro Pro Ile

85 90 95

Gly Lys Lys Lys Phe Glu Thr Leu Ser Tyr Leu Pro Pro Leu Thr Arg

100 105 110

Asp Met Thr Val Trp Gln Thr Leu Thr Phe Ala His Tyr Gln Pro Gln

115 120 125

Gln Trp Gly His Ser Ser Phe Leu His Arg Leu Phe Gly Ser Leu Arg

130 135 140

Ala Trp Arg Ala Ser Ser Gln Leu Leu Val Trp Ser Glu Ala Leu Gly

145 150 155 160

Gly Phe Leu Leu Ala Val Val Tyr Gly Ser Ala Pro Phe Val Pro Ser

165 170 175

Ser Ala Leu Gly Leu Gly Leu Ala Ala Ile Ala Ala Tyr Trp Ala Leu

180 185 190

Leu Ser Leu Thr Asp Ile Asp Leu Arg Gln Ala Thr Pro Ile His Trp

195 200 205

Leu Val Leu Leu Tyr Trp Gly Val Asp Ala Leu Ala Thr Gly Leu Ser

210 215 220

Pro Val Arg Ala Ala Ala Leu Val Gly Leu Ala Lys Leu Thr Leu Tyr

225 230 235 240

Leu Leu Val Phe Ala Leu Ala Ala Arg Val Leu Arg Asn Pro Arg Leu

245 250 255

Arg Ser Leu Leu Phe Ser Val Val Val Ile Thr Ser Leu Phe Val Ser

260 265 270

Val Tyr Gly Leu Asn Gln Trp Ile Tyr Gly Val Glu Glu Leu Ala Thr

275 280 285

Trp Val Asp Arg Asn Ser Val Ala Asp Phe Thr Ser Arg Val Tyr Ser

290 295 300

Tyr Leu Gly Asn Pro Asn Leu Leu Ala Ala Tyr Leu Val Pro Thr Thr

305 310 315 320

Ala Phe Ser Ala Ala Ala Ile Gly Val Trp Arg Gly Trp Leu Pro Lys

325 330 335

Leu Leu Ala Ile Ala Ala Thr Gly Ala Ser Ser Leu Cys Leu Ile Leu

340 345 350

Thr Tyr Ser Arg Gly Gly Trp Leu Gly Phe Val Ala Met Ile Phe Val

355 360 365

Trp Ala Leu Leu Gly Leu Tyr Trp Phe Gln Pro Arg Leu Pro Ala Pro

370 375 380

Trp Arg Arg Trp Leu Phe Pro Val Val Leu Gly Gly Leu Val Ala Val

385 390 395 400

Leu Leu Val Ala Val Leu Gly Leu Glu Pro Leu Arg Val Arg Val Leu

405 410 415

Ser Ile Phe Val Gly Arg Glu Asp Ser Ser Asn Asn Phe Arg Ile Asn

420 425 430

Val Trp Leu Ala Val Leu Gln Met Ile Gln Asp Arg Pro Trp Leu Gly

435 440 445

Ile Gly Pro Gly Asn Thr Ala Phe Asn Leu Val Tyr Pro Leu Tyr Gln

450 455 460

Gln Ala Arg Phe Thr Ala Leu Ser Ala Tyr Ser Val Pro Leu Glu Val

465 470 475 480

Ala Val Glu Gly Gly Leu Leu Gly Leu Thr Ala Phe Ala Trp Leu Leu

485 490 495

Leu Val Thr Ala Val Thr Ala Val Arg Gln Val Ser Arg Leu Arg Arg

500 505 510

Asp Arg Asn Pro Gln Ala Phe Trp Leu Met Ala Ser Leu Ala Gly Leu

515 520 525

Ala Gly Met Leu Gly His Gly Leu Phe Asp Thr Val Leu Tyr Arg Pro

530 535 540

Glu Ala Ser Thr Leu Trp Trp Leu Cys Ile Gly Ala Ile Ala Ser Phe

545 550 555 560

Trp Gln Pro Gln Pro Ser Lys Gln Leu Pro Pro Glu Ala Glu His Ser

565 570 575

Asp Glu Lys Met

580

<210> 11

<211> 974

<212> DNA

<213> 玉米(Zea mays)

<220>

<221> 启动子

<222> (1)..(974)

<223> ZmRbcS启动子

<400> 11

gagctccctt taatctggcg ctagatctgc atccgcggct tgcaaagata aatggcacat 60

ttagtgtgtt attttgcaat acctttcata gtagatatcc ttaaatgcag ttttaggcat 120

gtttgggtaa ttaaataaca tttttaggag gagttttaga tttacctttc tttcgtgatg 180

actgatgaca gacgtgggga attcaaatgc aactctagcg aaagttcata tatttttcat 240

aaatagctga ggctggggta attatttttt ttgtagaaaa atagaatagg tggaatggtt 300

ggggaaggcg taggcgctcg tggacgacgc ccgataaaag acaagaggcg gaattgccat 360

gaattcgagg tagctaagta aggcgcatat atatgccaaa aaattctact gtcactttcc 420

aatttcaatg cgctgccaaa caagccatcc tggaaactga cttgaattca gcccaattct 480

gtagatccaa acagggccgg cgtcagtgcc tcaggtgaga gagcagcaga cgatgcaaag 540

agccaaaact gcaagcagac gcagccgaag ccgaagccga agcccaagcc caaaactgtt 600

ttgtctttgc ccagaaccgc gacgagccta aactgcgctt cctcctatct acaagtccct 660

ggcacatcac gcatagtcca accatggcgc gcaggcgata aggcgcgcca cggggacgcg 720

acatgtggtg gcggacgcga tcaggatagg gccaggctgg ccgggcgcgg ccacgggaga 780

acggtggcca ctcgtcccac atccgcttcg tcctgtcctg tactgcgtcc tgcccccaac 840

gagagccgga gccggccatc ccgtcgcaca ctctccccct ctatatatgc cgtcggtgtg 900

ggggagccta ctacaggacg acccaagcaa gcaagcaagc agcgagtaca tacatactag 960

gcagccaggc agcc 974

<210> 12

<211> 439

<212> DNA

<213> 玉米(Zea mays)

<220>

<221> misc_feature

<222> (1)..(439)

<223> ZmRbcS 3'UTR

<400> 12

accgcgcccg ccggccgccc cccgccggct agctagctag ctagctcctg cgtgagctag 60

tagctagtgc catgcgtcgt ctctgtcgtt cggttttgct tcgggtcacc gtgtaccctt 120

tgcttgcttg gtttcttctt tccttttttc cttttttttt cttcttttcc ccggccatgg 180

ttcctttgct ttccagcagt tctctgctgg atgtgatgta tccattgttg caatcatggc 240

cttgcattgg ctacctctat acctgctaca aaactactgc aacgcctata tatacttggg 300

gtgaggaaca tgtgaatgca agctccggct atcatataca tgtaatatgg atacaaacta 360

tatatataaa tccgccgagg cgccgacaat actatacgac accgtgttaa gttaatatat 420

aactggtgct ttttattta 439

<210> 13

<211> 741

<212> DNA

<213> 双色高粱(Sorghum bicolor)

<220>

<221> 启动子

<222> (1)..(741)

<223> 4xRGCGR启动子

<400> 13

gaagcgagtg gcgcgctggc ggatgaggcg gcgagtggcc cggatgcacc ggcgcaggcg 60

agcgaagcga gtggcgcgct ggcggatgag gcggcgagtg gcccggatgc accggcgcag 120

gcgagcgaag cgagtggcgc gctggcggat gaggcggcga gtggcccgga tgcaccggcg 180

caggcgagcg aagcgagtgg cgcgctggcg gatgaggcgg cgagtggccc ggatgcaccg 240

gcgcaggcga gccgcacgcc gccgcccgcc gcggcgctcg cgcgcgcacc gctgccgcct 300

gccgccacac aatgcgagcg cgcgcgcaca cacacacaca ccacccgggc gggggggctg 360

tagtagtaac ggccttgtct tgtcggcacg cgcgcgtccg tgtgtataag gaggcaggcc 420

cgcgacaacg ataagcggca ctcgcacgat caatgtacac attgcccgtc cgcgccacca 480

catccagcat cgtcgccagc ctcgccaccc ccgcgccgtc ctcctcctcc ggctccggct 540

ccggccgccc caggcccagg ctcatccgga acgcccccgt cttcgccgcc cccgccaccg 600

tcgtgtaaac gggacggcgg gcagctgagg agtcaaacga gagagatcga gagaaagaaa 660

gggagggcat ccaccagccg ccggcgataa gaggggagga gagagaggcc agagaagagg 720

aggagaagaa gaagaaatcg a 741

<210> 14

<211> 287

<212> DNA

<213> 玉米(Zea mays)

<220>

<221> misc_feature

<222> (1)..(287)

<223> ZmCA1 3'UTR

<400> 14

gttcaaaact agggctacgg caattctacc ggcccgccga ctcctgcatc atcataaata 60

tatatactat actatactac tacgtaccta ccgatatgca cccgagcaat gtgaatgcgt 120

cgagtactat atatctgttt tctgcatcta catatatata ccggatcaat cgcccaatgt 180

gaatgtaata agcaatatca ttttctacca cttttcattc ctaacgctga gctttttatg 240

tactatatct tatatgatga ataataatat gaccgccttg tgatcta 287

<210> 15

<211> 295

<212> PRT

<213> 双色高粱(Sorghum bicolor)

<220>

<221> MISC_FEATURE

<222> (1)..(295)

<223> ABC转运体

<400> 15

Met Met Ala Gly Glu Gly Asn Gly Asp Glu Gly Trp Arg Arg Ser Gly

1 5 10 15

Ile Glu Val Ser Ala Leu Gln Phe Gly Tyr Asp Gly Gln Pro Pro Leu

20 25 30

Phe Ala Arg Phe Asn Leu Arg Val Ala Pro Gly Ser Arg Cys Leu Leu

35 40 45

Ile Gly Ala Asn Gly Ser Gly Lys Thr Thr Leu Leu Lys Ile Leu Ala

50 55 60

Gly Lys His Met Val Gly Gly Arg Asp Val Val Arg Val Leu Asn Gly

65 70 75 80

Ser Ala Phe His Asp Thr Gln Leu Val Cys Ser Gly Asp Leu Ser Tyr

85 90 95

Leu Gly Gly Ser Trp Ser Arg Thr Ile Gly Ser Ala Gly Asp Val Pro

100 105 110

Leu Gln Gly Asp Phe Ser Ala Glu His Met Ile Phe Gly Val Asp Gly

115 120 125

Val Asp Pro Val Arg Arg Glu Lys Leu Val Asp Leu Leu Asp Ile Asp

130 135 140

Leu Gln Trp Arg Met His Lys Val Ser Asp Gly Gln Arg Arg Arg Val

145 150 155 160

Gln Ile Cys Met Gly Leu Leu His Pro Tyr Lys Val Leu Leu Leu Asp

165 170 175

Glu Ile Thr Val Asp Leu Asp Val Val Thr Arg Met Asp Leu Leu Asp

180 185 190

Phe Phe Lys Glu Glu Cys Glu Gln Arg Glu Ala Thr Ile Val Tyr Ala

195 200 205

Thr His Ile Phe Asp Gly Leu Glu Thr Trp Ala Thr Asp Phe Ala Tyr

210 215 220

Ile Gln Glu Gly Glu Leu Arg Arg Ser Gly Arg Tyr Ser Asp Ile Glu

225 230 235 240

Glu Leu Lys Ser Ala Lys Asn Leu Leu Ser Val Val Glu Ser Trp Leu

245 250 255

Arg Ser Glu Thr Lys Leu Pro Lys Lys Glu Leu Pro Arg Pro Glu Thr

260 265 270

Gln Ala Arg Arg Ser Ser Pro Leu Asp Ala Ser Pro Phe Arg Ser Ser

275 280 285

Arg His Met Ala Tyr Tyr Arg

290 295

<210> 16

<211> 294

<212> PRT

<213> 小米(Setaria italica)

<220>

<221> MISC_FEATURE

<222> (1)..(294)

<223> ABC转运体

<400> 16

Met Thr Gly Glu Gly Asn Gly Asp Glu Gly Trp Arg Arg Ser Gly Ile

1 5 10 15

Glu Val Ser Ala Leu Gln Phe Gly Tyr Asp Gly Gln Ser Pro Leu Phe

20 25 30

Ala Arg Phe Asn Leu Arg Ile Ala Pro Gly Ser Arg Cys Leu Leu Val

35 40 45

Gly Ala Asn Gly Ser Gly Lys Thr Thr Leu Leu Lys Ile Leu Ala Gly

50 55 60

Lys His Met Val Gly Gly Arg Asp Val Val Arg Val Leu Asn Gly Ser

65 70 75 80

Ala Phe His Asp Thr Gln Leu Val Cys Asn Gly Glu Leu Ser Tyr Leu

85 90 95

Gly Gly Ser Trp Ser Gln Thr Ile Gly Ser Ala Gly Asp Val Pro Leu

100 105 110

Gln Gly Asp Phe Ser Ala Glu His Met Ile Phe Gly Val Asp Gly Val

115 120 125

Asp Pro Val Arg Arg Glu Lys Leu Val Asp Leu Leu Asp Ile Asp Leu

130 135 140

Gln Trp Arg Met His Lys Val Ser Asp Gly Gln Arg Arg Arg Val Gln

145 150 155 160

Ile Cys Met Gly Leu Leu His Pro Tyr Lys Val Leu Leu Leu Asp Glu

165 170 175

Ile Thr Val Asp Leu Asp Val Val Thr Arg Met Asp Leu Leu Asp Phe

180 185 190

Phe Lys Glu Glu Cys Glu Gln Arg Glu Ala Thr Ile Val Tyr Ala Thr

195 200 205

His Ile Phe Asp Gly Leu Glu Thr Trp Ala Thr Asp Ile Ala Tyr Ile

210 215 220

Gln Glu Gly Glu Leu Arg Lys Ser Ala Lys Tyr Ser Asp Ile Glu Glu

225 230 235 240

Leu Lys Ser Ala Glu Asn Leu Leu Ser Val Val Glu Ser Trp Leu Arg

245 250 255

Ser Glu Thr Lys Leu Pro Lys Lys Asp Pro Pro Arg Thr Glu Thr Gln

260 265 270

Pro Arg Arg Ser Ser Pro Phe Asp Ser Ser Pro Phe Arg Ser Ser Arg

275 280 285

His Met Ala Tyr Tyr Arg

290

<210> 17

<211> 292

<212> PRT

<213> 水稻(Oryza sativa)

<220>

<221> MISC_FEATURE

<222> (1)..(292)

<223> ABC转运体

<400> 17

Met Ala Gly Gly Glu Asp Glu Gly Trp Arg Arg Ser Gly Ile Glu Val

1 5 10 15

Ser Ala Leu Gln Phe Asp Tyr Asp Gly Gln Pro Pro Leu Phe Ala Arg

20 25 30

Phe Asn Leu Arg Ile Ala Pro Gly Ser Arg Cys Leu Leu Ile Gly Ala

35 40 45

Asn Gly Ser Gly Lys Thr Thr Leu Leu Lys Ile Leu Ala Gly Lys His

50 55 60

Met Val Gly Gly Arg Asp Val Val Arg Val Leu Asn Gly Ser Ala Phe

65 70 75 80

His Asp Thr Gln Leu Val Cys Asn Gly Asp Leu Ser Tyr Leu Gly Gly

85 90 95

Ser Trp Ser Arg Ala Ile Gly Ser Ala Gly Asp Val Pro Leu Gln Gly

100 105 110

Asp Phe Ser Ala Glu His Met Ile Phe Gly Val Asp Gly Val Asp Pro

115 120 125

Val Arg Arg Glu Lys Leu Val Asp Leu Leu Asp Ile Asp Leu Gln Trp

130 135 140

Arg Met His Lys Val Ser Asp Gly Gln Arg Arg Arg Val Gln Ile Cys

145 150 155 160

Met Gly Leu Leu His Pro Tyr Lys Val Leu Leu Leu Asp Glu Ile Thr

165 170 175

Val Asp Leu Asp Val Val Thr Arg Met Asp Leu Leu Asp Phe Phe Lys

180 185 190

Glu Glu Cys Glu Gln Arg Glu Ala Thr Ile Val Tyr Ala Thr His Ile

195 200 205

Phe Asp Gly Leu Glu Ser Trp Ala Thr Asp Ile Ala Tyr Ile Gln Glu

210 215 220

Gly Glu Leu Arg Lys Ser Ala Lys Tyr Ser Asp Val Glu Glu Leu Lys

225 230 235 240

Ser Ala Lys Asn Leu Leu Ser Val Val Glu Ser Trp Leu Arg Ser Glu

245 250 255

Thr Lys Leu Pro Lys Lys Glu His Pro Arg Pro Glu Thr Gln Pro Arg

260 265 270

Arg Ser Ser Pro Phe Asp Ala Ser Pro Phe Arg Ser Ser Arg His Met

275 280 285

Ala Tyr Tyr Arg

290

<210> 18

<211> 298

<212> PRT

<213> 短花稻(Oryza brachyantha)

<220>

<221> MISC_FEATURE

<222> (1)..(298)

<223> ABC转运体

<400> 18

Met Ala Gly Gly Gly Gly Gly Gly Gly Gly Gly Asp Glu Gly Trp Lys

1 5 10 15

Arg Ser Gly Ile Glu Val Ser Ala Leu Gln Phe Gly Tyr Asp Gly Gln

20 25 30

Pro Pro Leu Phe Ala Arg Phe Asp Leu Arg Val Ala Pro Gly Ser Arg

35 40 45

Cys Leu Leu Met Gly Ala Asn Gly Ser Gly Lys Thr Thr Leu Leu Lys

50 55 60

Ile Leu Ala Gly Lys His Met Val Gly Gly Arg Asp Val Val Arg Val

65 70 75 80

Leu Asn Gly Ser Ala Phe His Asp Thr Gln Leu Val Cys Asn Gly Asp

85 90 95

Leu Ser Tyr Leu Gly Gly Ser Trp Ser Arg Thr Ile Gly Ser Ala Gly

100 105 110

Asp Val Pro Leu Gln Gly Asp Phe Ser Ala Glu His Met Ile Phe Gly

115 120 125

Val Asp Gly Val Asp Pro Val Arg Arg Glu Lys Leu Val Asp Leu Leu

130 135 140

Asp Ile Asp Leu Gln Trp Arg Met His Lys Val Ser Asp Gly Gln Arg

145 150 155 160

Arg Arg Val Gln Ile Cys Met Gly Leu Leu His Pro Tyr Lys Val Leu

165 170 175

Leu Leu Asp Glu Ile Thr Val Asp Leu Asp Val Val Thr Arg Met Asp

180 185 190

Leu Leu Asp Phe Phe Lys Glu Glu Cys Glu Gln Arg Glu Ala Thr Ile

195 200 205

Val Tyr Ala Thr His Ile Phe Asp Gly Leu Glu Ser Trp Ala Thr Asp

210 215 220

Ile Ala Tyr Ile Gln Glu Gly Glu Leu Arg Lys Ser Ala Lys Tyr Ser

225 230 235 240

Asp Val Glu Glu Leu Lys Gly Ala Lys Asn Leu Leu Ser Val Val Glu

245 250 255

Ser Trp Leu Arg Ser Glu Thr Lys Leu Pro Lys Lys Glu His Pro Arg

260 265 270

Pro Glu Ile Gln Pro Arg Arg Ser Ser Pro Phe Asp Val Ser Pro Phe

275 280 285

Arg Ser Ser Arg His Met Ala Tyr Tyr Arg

290 295

<210> 19

<211> 294

<212> PRT

<213> 二穗短柄草(Brachypodium distachyon)

<220>

<221> MISC_FEATURE

<222> (1)..(294)

<223> ABC转运体

<400> 19

Met Ala Gly Gly Glu Gly Val Glu Glu Gly Trp Arg Lys Ser Gly Ile

1 5 10 15

Glu Val Ser Thr Leu Gln Phe Gly Tyr Asp Gly Gln Ala Pro Leu Phe

20 25 30

Ala Arg Phe Asn Leu Arg Val Ala Pro Gly Ser Arg Cys Leu Leu Val

35 40 45

Gly Ala Asn Gly Ser Gly Lys Thr Thr Leu Leu Lys Ile Leu Ala Gly

50 55 60

Lys His Met Val Gly Gly Arg Asp Val Val Arg Val Leu Asn Gly Ser

65 70 75 80

Ala Phe His Asp Thr Gln Phe Val Cys Ser Gly Asp Leu Ser Tyr Leu

85 90 95

Gly Gly Ser Trp Ser Arg Thr Ile Gly Ser Ala Gly Asp Val Pro Leu

100 105 110

Gln Gly Asp Phe Ser Ala Glu His Met Ile Phe Gly Val Asp Gly Val

115 120 125

Asp Pro Val Arg Arg Glu Lys Leu Ile Asp Leu Leu Asp Ile Asp Leu

130 135 140

Gln Trp Arg Met His Lys Val Ser Asp Gly Gln Arg Arg Arg Val Gln

145 150 155 160

Ile Cys Met Gly Leu Leu His Pro Tyr Lys Val Leu Leu Leu Asp Glu

165 170 175

Ile Thr Val Asp Leu Asp Val Val Thr Arg Met Asp Leu Leu Asp Phe

180 185 190

Phe Lys Glu Glu Cys Glu Gln Arg Glu Ala Thr Ile Val Tyr Ala Thr

195 200 205

His Ile Phe Asp Gly Leu Glu Thr Trp Ala Thr Asp Ile Ala Tyr Ile

210 215 220

Gln Glu Gly Glu Leu Arg Lys Ser Ala Lys Tyr Ser Asp Ile Glu Glu

225 230 235 240

Leu Lys Asn Ala Lys Asn Leu Leu Ser Val Val Glu Ser Trp Leu Lys

245 250 255

Ser Glu Thr Lys Leu Pro Lys Lys Glu Pro Ile Arg Ala Glu Ser Gln

260 265 270

Pro Arg Arg Ser Ser Pro Phe Asp Ala Ser Pro Phe Arg Ser Ser Arg

275 280 285

His Met Ala Tyr Tyr Arg

290

<210> 20

<211> 291

<212> PRT

<213> 乌拉尔图小麦(Triticum urartu)

<220>

<221> MISC_FEATURE

<222> (1)..(291)

<223> ABC转运体

<400> 20

Met Ala Gly Gly Glu Glu Gly Trp Arg Arg Ser Gly Ile Glu Val Ser

1 5 10 15

Thr Leu Gln Phe Gly Tyr Asp Gly Glu Ala Pro Leu Phe Ala Arg Phe

20 25 30

Asn Leu Arg Val Ala Pro Gly Ser Arg Cys Leu Leu Val Gly Ala Asn

35 40 45

Gly Ser Gly Lys Thr Thr Leu Leu Lys Ile Leu Ala Gly Lys His Met

50 55 60

Val Gly Gly Lys Asp Val Val Arg Val Leu Asn Gly Ser Ala Phe His

65 70 75 80

Asp Thr Gln Phe Val Cys Ser Gly Asp Leu Ser Tyr Leu Gly Gly Ser

85 90 95

Trp Ser Arg Asn Val Ser Ser Val Gly Asp Val Pro Leu Gln Gly Asp

100 105 110

Phe Ser Ala Glu His Met Ile Phe Gly Val Asp Gly Val Asp Pro Val

115 120 125

Arg Arg Glu Lys Leu Ile Asp Leu Leu Asp Ile Asp Leu Gln Trp Arg

130 135 140

Met His Lys Val Ser Asp Gly Gln Arg Arg Arg Val Gln Ile Cys Met

145 150 155 160

Gly Leu Leu His Pro Tyr Lys Val Leu Leu Leu Asp Glu Ile Thr Val

165 170 175

Asp Leu Asp Val Val Thr Arg Met Asp Leu Leu Asp Phe Phe Lys Glu

180 185 190

Glu Cys Glu Gln Arg Glu Ala Thr Ile Val Tyr Ala Thr His Ile Phe

195 200 205

Asp Gly Leu Glu Thr Trp Ala Thr Asp Ile Ala Tyr Ile Gln Glu Gly

210 215 220

Glu Leu Lys Lys Ser Ala Lys Tyr Ser Asp Ile Glu Glu Leu Lys Thr

225 230 235 240

Ala Lys Asn Leu Leu Thr Val Val Glu Ser Trp Leu Lys Ser Glu Thr

245 250 255

Lys Leu Pro Lys Lys Glu Pro Ala Arg Val Glu Ser Gln Pro Arg Arg

260 265 270

Ser Ser Pro Phe Asp Ala Ser Pro Phe Arg Ala Ser Arg His Met Ala

275 280 285

Tyr Tyr Arg

290

<210> 21

<211> 291

<212> PRT

<213> 大麦(Hordeum vulgare)

<220>

<221> MISC_FEATURE

<222> (1)..(291)

<223> ABC转运体

<400> 21

Met Ala Gly Gly Glu Glu Gly Trp Arg Arg Ser Gly Ile Glu Val Ser

1 5 10 15

Thr Leu Gln Phe Gly Tyr Asp Gly Glu Ala Pro Leu Phe Ala Arg Phe

20 25 30

Asn Leu Arg Val Ala Pro Gly Ser Arg Cys Leu Leu Val Gly Ala Asn

35 40 45

Gly Ser Gly Lys Thr Thr Leu Leu Lys Ile Leu Ala Gly Lys His Met

50 55 60

Val Gly Gly Lys Asp Val Val Arg Val Leu Asn Gly Ser Ala Phe His

65 70 75 80

Asp Thr Gln Phe Val Cys Ser Gly Asp Leu Ser Tyr Leu Gly Gly Ser

85 90 95

Trp Ser Arg Thr Val Ser Ser Val Gly Asp Val Pro Leu Gln Gly Asp

100 105 110

Phe Ser Ala Glu His Met Ile Phe Gly Val Asp Gly Val Asp Pro Val

115 120 125

Arg Arg Asp Lys Leu Ile Asp Leu Leu Asp Ile Asp Leu Gln Trp Arg

130 135 140

Met His Lys Val Ser Asp Gly Gln Arg Arg Arg Val Gln Ile Cys Met

145 150 155 160

Gly Leu Leu His Pro Tyr Lys Val Leu Leu Leu Asp Glu Ile Thr Val

165 170 175

Asp Leu Asp Val Val Thr Arg Met Asp Leu Leu Asp Phe Phe Lys Glu

180 185 190

Glu Cys Glu Gln Arg Glu Ala Thr Ile Val Tyr Ala Thr His Ile Phe

195 200 205

Asp Gly Leu Glu Thr Trp Ala Thr Asp Ile Ala Tyr Ile Gln Glu Gly

210 215 220

Glu Leu Lys Arg Ser Ala Lys Tyr Ser Asp Ile Glu Glu Leu Lys Thr

225 230 235 240

Ala Lys Asn Leu Leu Ser Val Val Glu Ser Trp Leu Lys Ser Glu Thr

245 250 255

Lys Leu Pro Lys Lys Glu Pro Ala Leu Val Glu Thr Gln Pro Arg Arg

260 265 270

Pro Ser Pro Phe Asp Ala Ser Pro Phe Arg Ala Ser Arg His Met Ala

275 280 285

Tyr Tyr Arg

290

<210> 22

<211> 291

<212> PRT

<213> 节节麦(Aegilops tauschii)

<220>

<221> MISC_FEATURE

<222> (1)..(291)

<223> ABC转运体

<400> 22

Met Ala Gly Gly Glu Glu Gly Trp Arg Arg Ser Gly Ile Glu Val Ser

1 5 10 15

Thr Leu Gln Phe Gly Tyr Asp Gly Glu Ala Pro Leu Phe Ala Arg Phe

20 25 30

Asn Leu Arg Val Ala Pro Gly Ser Arg Cys Leu Leu Val Gly Ala Asn

35 40 45

Gly Ser Gly Lys Thr Thr Leu Leu Lys Ile Leu Ala Gly Lys His Met

50 55 60

Val Gly Gly Lys Asp Val Val Arg Val Leu Asn Gly Ser Ala Phe His

65 70 75 80

Asp Thr Gln Phe Val Cys Ser Gly Asp Leu Ser Tyr Leu Gly Gly Ser

85 90 95

Trp Ser Arg Asn Val Ser Ser Val Gly Asp Val Pro Leu Gln Gly Asp

100 105 110

Phe Ser Ala Glu His Met Ile Phe Gly Val Asp Gly Val Asp Pro Val

115 120 125

Arg Arg Glu Lys Leu Ile Asp Leu Leu Asp Ile Asp Leu Gln Trp Arg

130 135 140

Met His Lys Val Ser Asp Gly Gln Arg Arg Arg Val Gln Ile Cys Met

145 150 155 160

Gly Leu Leu His Pro Tyr Lys Val Leu Leu Leu Asp Glu Ile Thr Val

165 170 175

Asp Leu Asp Val Val Thr Arg Met Asp Leu Leu Asp Phe Phe Lys Glu

180 185 190

Glu Cys Glu Gln Arg Glu Ala Thr Ile Val Tyr Ala Thr His Ile Phe

195 200 205

Asp Gly Leu Glu Thr Trp Ala Thr Asp Ile Ala Tyr Ile Gln Glu Gly

210 215 220

Glu Leu Lys Lys Ser Ala Lys Tyr Ser Asp Ile Glu Glu Leu Lys Thr

225 230 235 240

Ala Lys Asn Leu Leu Ser Val Val Glu Ser Trp Leu Lys Ser Glu Ala

245 250 255

Lys Leu Pro Lys Lys Glu Pro Ala Arg Val Glu Ser Gln Pro Arg Arg

260 265 270

Pro Ser Pro Phe Asp Ala Ser Pro Phe Arg Ala Ser Arg His Met Ala

275 280 285

Tyr Tyr Arg

290

<210> 23

<211> 286

<212> PRT

<213> 油棕(Elaeis guineensis)

<220>

<221> MISC_FEATURE

<222> (1)..(286)

<223> ABC转运体

<400> 23

Met Ala Glu Ser Thr Gly Gly Ile Glu Val Ser Gly Leu Gln Phe Ala

1 5 10 15

Tyr Glu Gly Gln Pro Leu Leu Phe Thr Lys Phe Asn Leu Glu Ile Ser

20 25 30

Pro Gly Ser Arg Cys Leu Leu Ile Gly Ala Asn Gly Ser Gly Lys Thr

35 40 45

Thr Leu Leu Lys Ile Leu Ala Gly Lys His Met Val Gly Gly Arg Asp

50 55 60

Val Val Arg Val Leu Asn Cys Ser Ala Phe His Asp Thr Gln Leu Val

65 70 75 80

Cys Ser Gly Asp Leu Ala Tyr Leu Gly Gly Ser Trp Ser Lys Thr Ile

85 90 95

Gly Ser Ala Gly Asp Val Pro Leu Gln Gly Asp Phe Ser Ala Glu His

100 105 110

Met Ile Phe Gly Val Glu Gly Ile Asp Pro Val Arg Arg Glu Lys Leu

115 120 125

Ile Asp Leu Leu Asp Ile Asp Leu Gln Trp Arg Met His Lys Val Ser

130 135 140

Asp Gly Gln Arg Arg Arg Val Gln Ile Cys Met Gly Leu Leu His Pro

145 150 155 160

Tyr Lys Val Leu Leu Leu Asp Glu Val Thr Val Asp Leu Asp Val Val

165 170 175

Thr Arg Leu Asp Leu Leu Asp Phe Phe Arg Glu Glu Cys Glu Glu Arg

180 185 190

Gly Ala Thr Ile Val Tyr Ala Thr His Ile Phe Asp Gly Leu Glu Thr

195 200 205

Trp Ala Thr Asp Val Ala Tyr Val Gln Asp Gly Glu Leu Arg Arg Ser

210 215 220

Gly Lys Leu Ser Asp Ile His Glu Leu Lys Gly Ser Asn Asn Leu Leu

225 230 235 240

Ser Val Val Glu Ser Trp Leu Arg Ser Glu Ser Lys Asn Pro Arg Lys

245 250 255

Gln Ser Ile Ile Ser Ser Val Gln Leu Ser Arg Gly Ser Pro Phe Asp

260 265 270

Ser Ser Pro Phe Arg Ser Ser Arg His Met Ala Tyr Tyr Arg

275 280 285

<210> 24

<211> 283

<212> PRT

<213> 芦笋(Asparagus officinalis)

<220>

<221> MISC_FEATURE

<222> (1)..(283)

<223> ABC转运体

<400> 24

Met Gly Gly Gly Ile Glu Val Ser Gly Leu Gln Phe Ala Tyr Asp Gly

1 5 10 15

Gln Pro Pro Ile Phe Ala Gln Phe Asn Leu Asn Ile Ala Pro Gly Ser

20 25 30

Arg Cys Leu Leu Ile Gly Ala Asn Gly Ser Gly Lys Thr Thr Leu Leu

35 40 45

Lys Ile Leu Ala Gly Lys His Met Val Gly Gly Arg Asp Val Val Gln

50 55 60

Val Leu Ser Cys Ser Ala Phe His Asp Thr Gln Leu Val Cys Ser Gly

65 70 75 80

Asp Leu Ala Tyr Leu Gly Glu Ser Trp Ser Lys Thr Val Ser Ala Ala

85 90 95

Gly Asp Val Pro Leu Gln Gly Asp Phe Ser Ala Glu His Met Ile Phe

100 105 110

Gly Val Glu Gly Ile Asp Pro Leu Arg Arg Glu Lys Leu Ile Asp Leu

115 120 125

Leu Asp Ile Asp Leu Gln Trp Arg Met His Lys Val Ser Asp Gly Gln

130 135 140

Arg Arg Arg Val Gln Ile Cys Met Gly Leu Leu His Pro Tyr Lys Val

145 150 155 160

Leu Leu Leu Asp Glu Val Thr Val Asp Leu Asp Val Val Thr Arg Met

165 170 175

Asp Leu Leu Glu Phe Phe Lys Glu Glu Cys Glu Gln Arg Gly Ala Thr

180 185 190

Ile Val Tyr Cys Thr His Ile Phe Asp Gly Leu Glu Thr Trp Ala Thr

195 200 205

His Leu Ala Tyr Ile Gln Glu Gly Glu Leu Arg Arg Cys Thr Lys Met

210 215 220

Glu Asp Ile His Glu Leu Glu Lys Ala Asp Asn Leu Leu Ser Val Val

225 230 235 240

Glu Ser Trp Leu Arg Ser Glu Thr Lys Leu Pro Lys Lys Glu Pro Ile

245 250 255

Lys Ser Ser Ile Leu Ser Arg Gly Ala Ser Pro Phe Asp Ser Ser Pro

260 265 270

Phe Lys Ser Ser Arg His Met Ala Tyr Tyr Arg

275 280

<210> 25

<211> 286

<212> PRT

<213> 海枣(Phoenix dactylifera)

<220>

<221> MISC_FEATURE

<222> (1)..(286)

<223> ABC转运体

<400> 25

Met Val Glu Ser Thr Gly Gly Ile Glu Val Ser Gly Leu Gln Phe Ala

1 5 10 15

Tyr Glu Gly Gln Pro Pro Leu Phe Thr Lys Phe Asn Leu Glu Ile Ser

20 25 30

Pro Gly Asp Arg Cys Leu Leu Ile Gly Ala Asn Gly Ser Gly Lys Thr

35 40 45

Thr Leu Leu Lys Ile Leu Ala Gly Lys His Met Val Gly Gly Arg Asp

50 55 60

Val Val Arg Val Leu Asn Arg Ser Ala Phe His Asp Thr Gln Leu Val

65 70 75 80

Cys Ser Gly Asp Leu Ala Tyr Leu Gly Gly Ser Trp Ser Lys Thr Ile

85 90 95

Gly Ser Ala Gly Asp Val Pro Leu Gln Gly Asp Phe Ser Ala Glu His

100 105 110

Met Ile Phe Gly Val Glu Gly Ile Asp Pro Ala Arg Arg Glu Lys Leu

115 120 125

Val Ser Leu Leu Asp Ile Asp Leu Gln Trp Arg Met His Arg Val Ser

130 135 140

Asp Gly Gln Arg Arg Arg Val Gln Ile Cys Met Gly Leu Leu His Pro

145 150 155 160

Tyr Lys Val Leu Leu Leu Asp Glu Val Thr Val Asp Leu Asp Val Val

165 170 175

Thr Arg Met Asp Leu Leu Asp Phe Phe Lys Glu Glu Cys Glu Gln Arg

180 185 190

Gly Ala Thr Ile Val Tyr Ala Thr His Ile Phe Asp Gly Leu Glu Thr

195 200 205

Trp Ala Thr Asp Val Ala Tyr Ile Gln Asp Gly Glu Leu Arg Arg Phe

210 215 220

Gly Lys Leu Ser Gly Ile His Glu Leu Lys Gly Ser Asn Asn Leu Leu

225 230 235 240

Ser Val Val Glu Ser Trp Leu Leu Ser Glu Ser Lys Asn Pro Arg Lys

245 250 255

Gln Pro Val Asn Ser Ser Val Gln Ser Ser Arg Gly Ser Pro Phe Asp

260 265 270

Ser Ser Pro Phe Arg Ser Ser Arg His Met Ala Tyr Tyr Arg

275 280 285

<210> 26

<211> 291

<212> PRT

<213> 菠萝(Ananas comosus)

<220>

<221> MISC_FEATURE

<222> (1)..(291)

<223> ABC转运体

<400> 26

Met Val Glu Glu Glu Gly Trp Arg Arg Ser Gly Ile Glu Val Arg Gly

1 5 10 15

Leu His Phe Ala Tyr Asp Gly Gln Pro Pro Leu Phe Val Ser Phe Asp

20 25 30

Leu Glu Val Ala Pro Gly Ser Arg Cys Leu Leu Ile Gly Ala Asn Gly

35 40 45

Ser Gly Lys Thr Thr Leu Leu Lys Ile Leu Ala Gly Lys His Met Val

50 55 60

Gly Gly Arg Asp Val Val Arg Val Leu Asn Ser Ser Ala Phe His Asp

65 70 75 80

Thr Glu Leu Val Cys Asn Gly Asp Leu Ala Tyr Leu Gly Gly Ser Trp

85 90 95

Ser Lys Thr Ile Gly Ser Ala Gly Asp Val Pro Leu Gln Gly Asp Phe

100 105 110

Ser Ala Glu His Met Ile Phe Gly Val Glu Gly Val Asp Pro Val Arg

115 120 125

Arg Glu Lys Leu Ile Asp Leu Leu Asp Ile Asp Leu Asp Trp Arg Met

130 135 140

His Lys Val Ser Asp Gly Gln Arg Arg Arg Val Gln Ile Cys Met Gly

145 150 155 160

Leu Leu His Pro Tyr Lys Val Leu Leu Leu Asp Glu Val Thr Val Asp

165 170 175

Leu Asp Val Val Thr Arg Met Asp Leu Leu Asp Phe Phe Lys Glu Glu

180 185 190

Cys Glu Gln Arg Glu Ala Thr Ile Val Tyr Ala Thr His Ile Phe Asp

195 200 205

Gly Leu Glu Ser Trp Ala Thr His Val Ala Tyr Ile Gln Asp Gly Glu

210 215 220

Leu Arg Arg Ser Ala Lys Met Ser Asp Val His Glu Leu Lys Ser Ala

225 230 235 240

Glu Asn Leu Leu Ser Val Val Glu Ser Trp Leu His Ser Glu Val Lys

245 250 255

Arg Pro Ile Glu Lys Glu Ser Val Asn Ser Thr Leu Gln Ser Leu Lys

260 265 270

Ser Ser Pro Phe Asp Gln Ser Pro Phe Arg Ser Ser Arg His Met Ala

275 280 285

Tyr Tyr Arg

290

<210> 27

<211> 287

<212> PRT

<213> 小果野蕉(Musa acuminate)

<220>

<221> MISC_FEATURE

<222> (1)..(287)

<223> ABC转运体

<400> 27

Met Glu Gly Gly Gly Ile Glu Val Ser Ser Leu Asn Phe Ala Tyr Glu

1 5 10 15

Gly Leu Pro Pro Leu Phe Thr Arg Phe Asn Leu Asp Val Ser Pro Gly

20 25 30

Ser Arg Cys Leu Leu Val Gly Ala Asn Gly Ser Gly Lys Thr Thr Leu

35 40 45

Leu Lys Ile Leu Ala Gly Lys His Met Val Gly Gly Arg Asp Val Val

50 55 60

Arg Val Leu Asn Ala Ser Ala Phe His Asp Thr Arg Leu Val Cys Ser

65 70 75 80

Gly Asp Leu Ala Tyr Leu Gly Gly Ser Trp Ser Lys Thr Val Gly Ser

85 90 95

Ala Gly Asp Ile Pro Leu Gln Gly Asp Phe Ser Ala Glu His Met Ile

100 105 110

Phe Gly Val Glu Gly Ile Asp Pro Ala Arg Arg Glu Lys Leu Ile Asp

115 120 125

Leu Leu Asp Ile Asn Leu Gln Trp Arg Met His Lys Val Ser Asp Gly

130 135 140

Gln Arg Arg Arg Val Gln Ile Cys Met Gly Leu Leu Tyr Pro Tyr Lys

145 150 155 160

Val Leu Leu Leu Asp Glu Val Thr Val Asp Leu Asp Val Val Thr Arg

165 170 175

Met Asp Leu Leu Glu Phe Phe Thr Glu Glu Cys Glu Gln Arg Gly Val

180 185 190

Ile Ile Val Tyr Ala Thr His Ile Phe Asp Gly Leu Glu Thr Trp Ala

195 200 205

Thr His Val Ala Tyr Ile Gln Asp Gly Asp Leu Lys Arg Ser Gly Lys

210 215 220

Leu Ser Asp Ile Ile Glu Leu Lys Ser Ala Asn Asn Leu Leu Ser Val

225 230 235 240

Val Glu Ser Trp Leu Arg Ser Glu Ser Lys Leu Pro Lys Lys Glu Leu

245 250 255

Ile Lys Pro Ile Ser Ala Val Ala Arg Ser Ser Thr Leu Ser Pro Phe

260 265 270

Asp Ser Ser Pro Phe Arg Ser Ser Arg His Met Ala Tyr Tyr Arg

275 280 285

<210> 28

<211> 286

<212> PRT

<213> 桃红蝴蝶兰(Phalaenopsis equestris)

<220>

<221> MISC_FEATURE

<222> (1)..(286)

<223> ABC转运体

<400> 28

Met Ala Glu Thr Gly Gly Gly Ile Glu Val Thr Gly Met Gln Phe Ala

1 5 10 15

Tyr Asp Gly Arg Gln Arg Ile Phe Ala Arg Phe Ser Leu Glu Val Ser

20 25 30

Pro Gly Ser Arg Cys Leu Leu Ile Gly Ala Asn Gly Ser Gly Lys Thr

35 40 45

Thr Leu Leu Arg Ile Leu Ala Gly Lys His Met Val Gly Gly Arg Asn

50 55 60

Val Val Arg Val Leu Asn Cys Ser Ala Phe His Asp Thr His Leu Val

65 70 75 80

Cys Ser Gly Asp Leu Ala Tyr Leu Gly Glu Ser Trp Ser Lys Thr Val

85 90 95

Gly Ser Val Gly Asp Val Pro Leu Gln Gly Asp Phe Ser Ala Glu His

100 105 110

Met Ile Phe Gly Val Glu Gly Ala Asp Pro Leu Arg Arg Glu Lys Leu

115 120 125

Ile Asp Leu Leu Asp Ile Asn Leu Gln Trp Arg Met His Lys Val Ser

130 135 140

Asp Gly Gln Arg Arg Arg Val Gln Ile Cys Met Gly Leu Leu Arg Pro

145 150 155 160

Tyr Lys Val Leu Leu Leu Asp Glu Val Thr Val Asp Leu Asp Val Val

165 170 175

Thr Arg Met Asp Leu Leu Asp Phe Phe Lys Glu Glu Cys Glu Gln Arg

180 185 190

Gly Ala Thr Ile Val Tyr Ala Thr His Ile Phe Asp Gly Leu Glu Thr

195 200 205

Trp Ala Thr Asp Leu Ala Tyr Ile Gln Glu Gly Glu Leu Lys Arg Leu

210 215 220

Ala Lys Leu Ala Glu Ile Lys Glu Leu Lys Glu Ala Lys Asn Leu Leu

225 230 235 240

Ser Val Val Glu Ser Trp Leu Arg Ser Glu Thr Lys Leu Pro Lys Lys

245 250 255

Glu Pro Phe Leu Ser Pro Ala Gln Thr Thr Val Ser Ser Pro Phe Asp

260 265 270

Ala Ser Pro Phe Arg Ser Ser Arg His Met Ala Tyr Tyr Arg

275 280 285

<210> 29

<211> 287

<212> PRT

<213> 铁皮石斛(Dendrobium catenatum)

<220>

<221> MISC_FEATURE

<222> (1)..(287)

<223> ABC转运体

<400> 29

Met Ala Glu Ser Gly Gly Gly Ile Glu Val Thr Ser Met Gln Phe Ala

1 5 10 15

Tyr Asp Gly Arg Gln Pro Ile Phe Ala Arg Phe Ser Leu Lys Val Ser

20 25 30

Pro Gly Ser Arg Cys Leu Leu Ile Gly Ala Asn Gly Ser Gly Lys Thr

35 40 45

Thr Leu Leu Arg Ile Leu Ala Gly Lys His Met Val Gly Gly Arg Asp

50 55 60

Val Val Arg Val Leu Asn Cys Ser Ala Phe His Asp Thr His Leu Val

65 70 75 80

Cys Ser Gly Asp Leu Ala Tyr Leu Gly Glu Ser Trp Ser Lys Thr Val

85 90 95

Gly Ser Thr Gly Asp Val Pro Leu Gln Gly Asp Phe Ser Ala Glu His

100 105 110

Met Ile Phe Gly Val Glu Gly Ala Asp Pro Leu Arg Arg Glu Lys Leu

115 120 125

Ile Asp Leu Leu Asp Ile Asn Leu Gln Trp Arg Met His Lys Val Ser

130 135 140

Asp Gly Gln Arg Arg Arg Val Gln Ile Cys Ile Gly Leu Leu Gln Pro

145 150 155 160

Tyr Lys Val Leu Leu Leu Asp Glu Val Thr Val Asp Leu Asp Val Val

165 170 175

Thr Arg Met Asp Leu Leu Asp Phe Phe Lys Glu Glu Cys Glu Gln Arg

180 185 190

Gly Ala Thr Ile Val Tyr Ala Thr His Ile Phe Asp Gly Leu Glu Ala

195 200 205

Trp Ala Thr Asp Leu Ala Tyr Ile Gln Glu Gly Glu Leu Arg Arg Leu

210 215 220

Ala Lys Val Ala Asp Ile Gln Glu Leu Lys Asp Ala Lys Asn Leu Leu

225 230 235 240

Ser Val Val Glu Ser Trp Leu Arg Ser Glu Thr Lys Leu Pro Lys Lys

245 250 255

Glu Leu Ile Gly Phe His Ala Lys Ser Thr Gly Ser Ser Ser Pro Phe

260 265 270

Asp Ala Ser Pro Phe Arg Ser Ser Arg His Met Ala Tyr Tyr Arg

275 280 285

<210> 30

<211> 289

<212> PRT

<213> 胡萝卜(Daucus carota)

<220>

<221> MISC_FEATURE

<222> (1)..(289)

<223> ABC转运体

<400> 30

Met Ala Arg Glu Gly Asp Glu Ser Asn Gly Ile Arg Val Lys Gly Met

1 5 10 15

Gln Phe Gly Tyr Asp Ile Gln Ile Pro Leu Phe Val Asp Phe Thr Leu

20 25 30

Asp Ile Ser Pro Gly Ser Arg Cys Leu Leu Val Gly Ala Asn Gly Ser

35 40 45

Gly Lys Thr Thr Leu Leu Lys Ile Leu Ala Gly Lys His Met Val Gly

50 55 60

Gly Arg Asp Val Val Gln Val Leu Asn Cys Ser Ala Phe His Asp Thr

65 70 75 80

His Leu Val Cys Ser Gly Asp Leu Ser Tyr Leu Gly Gly Ser Trp Thr

85 90 95

Lys Thr Val Gly Ser Ala Gly Glu Ile Pro Leu Gln Gly Asp Phe Ser

100 105 110

Ala Glu His Met Ile Phe Gly Val Glu Gly Ile Asp Pro Val Arg Arg

115 120 125

Glu Lys Leu Ile Asp Leu Leu Asp Ile Asp Leu Arg Trp Arg Met His

130 135 140

Lys Val Ser Asp Gly Gln Arg Arg Arg Val Gln Ile Cys Met Gly Leu

145 150 155 160

Leu His Pro Phe Gln Val Leu Leu Leu Asp Glu Val Thr Val Asp Leu

165 170 175

Asp Val Val Ala Arg Met Asp Leu Leu Asp Phe Phe Lys Glu Glu Cys

180 185 190

Glu Gln Arg Gly Ala Thr Ile Val Tyr Ala Thr His Ile Phe Asp Gly

195 200 205

Leu Glu Thr Trp Ala Thr Asp Leu Ala Tyr Ile Gln Asp Gly Glu Leu

210 215 220

Arg Arg Thr Asp Lys Leu Ser Glu Leu Ala Glu Leu Lys His Ala Ala

225 230 235 240

Asn Leu Leu Ser Val Val Glu Ser Trp Leu Arg Ser Glu Thr Lys Ile

245 250 255

Glu Lys Lys Lys Pro Val Ser Asn Ser Ser Gln Val Arg Lys Ser Ser

260 265 270

Pro Phe Asp Ser Ser Pro Phe Arg Ser Thr Arg His Met Ala Tyr Tyr

275 280 285

Arg

<210> 31

<211> 287

<212> PRT

<213> 茶(Camellia sinensis)

<220>

<221> MISC_FEATURE

<222> (1)..(287)

<223> ABC转运体

<400> 31

Met Glu Glu Lys Asn Trp Ser Ser Ile Glu Val Ser Gly Met Gln Phe

1 5 10 15

Gly Tyr Asp Gly Gln Ser Pro Val Phe Ile Asp Phe Thr Leu Lys Ile

20 25 30

Leu Pro Gly Ser Arg Cys Leu Leu Val Gly Ala Asn Gly Ser Gly Lys

35 40 45

Thr Thr Leu Leu Lys Ile Leu Ala Gly Lys His Met Val Gly Gly Arg

50 55 60

Asp Val Val Arg Val Leu Asn Cys Ser Ala Phe His Asp Thr His Leu

65 70 75 80

Val Cys Ser Gly Asp Leu Ser Tyr Leu Gly Gly Ser Trp Ser Lys Thr

85 90 95

Val Gly Ser Ala Gly Glu Ile Pro Leu Gln Gly Asp Phe Ser Ala Glu

100 105 110

His Met Ile Phe Gly Val Glu Gly Val Asp Pro Val Arg Arg Glu Lys

115 120 125

Leu Ile Glu Leu Leu Asp Ile Asp Leu Gln Trp Arg Met His Lys Val

130 135 140

Ser Asp Gly Gln Arg Arg Arg Val Gln Ile Cys Met Gly Leu Leu His

145 150 155 160

Pro Phe Gln Val Leu Leu Leu Asp Glu Val Thr Val Asp Leu Asp Val

165 170 175

Val Ala Arg Leu Asp Leu Leu Asp Phe Phe Lys Glu Glu Cys Glu Gln

180 185 190

Arg Gly Ala Thr Ile Val Tyr Ala Thr His Ile Phe Asp Gly Leu Glu

195 200 205

Thr Trp Ala Thr Asp Leu Ala Tyr Ile His Ala Gly Glu Leu Lys Arg

210 215 220

Thr Asp Lys Leu Ser Glu Leu Gly Glu Leu Lys Asn Ser Ala Asn Leu

225 230 235 240

Leu Ser Val Val Glu Ser Trp Leu Arg Ser Glu Thr Asn Thr Glu Lys

245 250 255

Lys Lys Pro Thr Asn Pro Pro Val Glu Asn Gln Lys Thr Ser Pro Phe

260 265 270

Asp Ser Ser Pro Phe Arg Ser Ser Arg His Met Ala Tyr Tyr Arg

275 280 285

<210> 32

<211> 291

<212> PRT

<213> 葡萄(Vitis vinifera)

<220>

<221> MISC_FEATURE

<222> (1)..(291)

<223> ABC转运体

<400> 32

Met Ala Ile Gln Ser Ser Ser Ser Ser Asn Ser Ile Lys Val Asn Gly

1 5 10 15

Leu Gln Phe Ala Tyr Glu Gly Gln Pro Pro Leu Phe Leu Asp Phe Asn

20 25 30

Leu Gln Ile Ser Pro Gly Ser Arg Cys Leu Leu Val Gly Ala Asn Gly

35 40 45

Ser Gly Lys Thr Thr Leu Leu Arg Ile Leu Ala Gly Lys His Met Val

50 55 60

Gly Gly Arg Asp Val Val Gln Val Leu Asn Cys Ser Ala Phe His Asp

65 70 75 80

Thr His Leu Val Cys Ser Gly Asp Leu Ser Tyr Leu Gly Gly Ser Trp

85 90 95

Ser Lys Asn Ile Gly Ser Ala Gly Glu Val Pro Leu Gln Gly Asp Phe

100 105 110

Ser Ala Glu His Met Ile Phe Gly Val Glu Gly Ile Asn Pro Val Arg

115 120 125

Arg Asp Lys Leu Val Asp Leu Leu Asp Ile Asp Leu Gln Trp Arg Met

130 135 140

His Lys Val Ser Asp Gly Gln Arg Arg Arg Val Gln Ile Cys Met Gly

145 150 155 160

Leu Leu His Pro Phe Gln Val Leu Leu Leu Asp Glu Val Thr Val Asp

165 170 175

Leu Asp Val Val Ala Arg Met Asp Leu Leu Glu Phe Phe Lys Glu Glu

180 185 190

Cys Asp Gln Arg Gly Ala Thr Ile Val Tyr Ala Thr His Ile Phe Asp

195 200 205

Gly Leu Glu Thr Trp Ala Thr Asp Leu Ala Tyr Val Gln Glu Gly Asp

210 215 220

Leu Arg Lys Ile Glu Lys Leu Ser Glu Leu Asp Glu Leu Lys Thr Ser

225 230 235 240

Ala Asn Leu Leu Ser Val Val Glu Ser Trp Leu Arg Ser Glu Thr Lys

245 250 255

Cys Glu Lys Lys Lys Pro Ile Asn Pro Pro Ala Gln Ile Gln Arg Thr

260 265 270

Pro Ser Pro Phe Asp Ser Ser Pro Phe Arg Ser Ser Arg His Met Ala

275 280 285

Tyr Tyr Arg

290

<210> 33

<211> 299

<212> PRT

<213> 可可(Theobroma cacao)

<220>

<221> MISC_FEATURE

<222> (1)..(299)

<223> ABC转运体

<400> 33

Met Ala Glu Gln Ala Cys Cys Lys Ser Arg Glu Glu Glu Gly Asn Gly

1 5 10 15

Glu Ser Lys Gly Ile Arg Val Cys Gly Met Gln Phe Ala Tyr Glu Leu

20 25 30

Gln Pro Pro Leu Phe Val Asp Phe Asp Val Asp Ile Ala Pro Gly Ser

35 40 45

Arg Cys Leu Leu Val Gly Ala Asn Gly Ser Gly Lys Thr Thr Leu Leu

50 55 60

Lys Ile Leu Ala Gly Lys His Met Val Gly Gly Arg Asp Val Val Arg

65 70 75 80

Val Leu Asn Arg Ser Ala Phe His Asp Thr Gln Leu Val Cys Ser Gly

85 90 95

Asp Leu Ala Tyr Leu Gly Gly Ser Trp Ser Lys Thr Ile Gly Ser Ala

100 105 110

Gly Glu Val Pro Leu Gln Gly Asp Phe Ser Ala Glu His Met Ile Phe

115 120 125

Gly Val Glu Gly Ile Asp Pro Val Arg Arg Gly Lys Leu Ile Glu Leu

130 135 140

Leu Asp Ile Asp Leu Gln Trp Arg Met His Lys Val Ser Asp Gly Gln

145 150 155 160

Arg Arg Arg Val Gln Ile Cys Met Gly Leu Leu His Pro Phe Gln Val

165 170 175

Leu Leu Leu Asp Glu Val Thr Val Asp Leu Asp Val Val Ala Arg Met

180 185 190

Asp Leu Leu Asp Phe Phe Lys Glu Glu Cys Glu Gln Arg Gly Ala Thr

195 200 205

Ile Val Tyr Ala Thr His Ile Phe Asp Gly Leu Glu Thr Trp Ala Thr

210 215 220

His Leu Ala Tyr Ile Gln Asp Gly Glu Leu Lys Arg Ser Glu Lys Leu

225 230 235 240

Thr Glu Val Asn Glu Leu Lys Ser Ser Glu Asn Leu Leu Ser Val Val

245 250 255

Glu Ala Trp Leu Arg Ser Glu Thr Lys Cys Glu Lys Lys Lys Pro Ile

260 265 270

Lys Thr Pro Ala Gln Val Gln Lys Ala Ser Pro Phe Gly Ile Ser Pro

275 280 285

Phe Met Ser Ser Arg His Met Ala Tyr Tyr Arg

290 295

<210> 34

<211> 299

<212> PRT

<213> 黄麻(Corchorus capsularis)

<220>

<221> MISC_FEATURE

<222> (1)..(299)

<223> ABC转运体

<400> 34

Met Ala Glu Gln Thr Phe Cys Lys Ser Arg Glu Gly Asp Glu Asn Ala

1 5 10 15

Glu Ser Lys Ser Ile Arg Val Cys Gly Met Gln Phe Ala Tyr Glu Leu

20 25 30

Gln His Pro Leu Phe Val Asp Phe Asn Leu Glu Ile Ala Pro Gly Ser

35 40 45

Arg Cys Leu Leu Val Gly Ala Asn Gly Ser Gly Lys Thr Thr Leu Leu

50 55 60

Lys Ile Leu Ala Gly Lys His Met Val Gly Gly Arg Asp Val Val Arg

65 70 75 80

Val Leu Asn Arg Ser Ala Phe His Asp Thr Gln Leu Val Cys Gly Gly

85 90 95

Asp Leu Ala Tyr Leu Gly Gly Ser Trp Ser Lys Thr Ile Gly Ser Ala

100 105 110

Gly Glu Val Pro Leu Gln Gly Asp Phe Ser Ala Glu His Met Ile Phe

115 120 125

Gly Val Glu Gly Ile Asp Pro Val Arg Arg Glu Lys Leu Ile Glu Leu

130 135 140

Leu Asp Ile Asp Leu Gln Trp Arg Met His Lys Val Ser Asp Gly Gln

145 150 155 160

Arg Arg Arg Val Gln Ile Cys Met Gly Leu Leu His Pro Phe Gln Val

165 170 175

Leu Leu Leu Asp Glu Val Thr Val Asp Leu Asp Val Val Ala Arg Met

180 185 190

Asp Leu Leu Asp Phe Phe Lys Glu Glu Cys Glu Gln Arg Gly Ala Thr

195 200 205

Val Val Tyr Ala Thr His Ile Phe Asp Gly Leu Glu Thr Trp Ala Thr

210 215 220

His Leu Ala Tyr Ile Gln Asp Gly Glu Leu Lys Lys Ser Glu Lys Leu

225 230 235 240

Thr Glu Ile Asn Glu Leu Lys Ser Ser Glu Asn Leu Leu Ser Val Val

245 250 255

Glu Ala Trp Leu Arg Ser Glu Thr Lys Cys Glu Lys Lys Lys Ala Val

260 265 270

Asn Asn Pro Ala Gln Val Gln Lys Thr Ser Pro Phe Gly Thr Ser Pro

275 280 285

Phe Met Ser Ser Arg His Met Ala Tyr Tyr Arg

290 295

<210> 35

<211> 295

<212> PRT

<213> 川桑(Morus notabilis)

<220>

<221> MISC_FEATURE

<222> (1)..(295)

<223> ABC转运体

<400> 35

Met Ala Asp Gly Glu Glu Asp Gln Arg Asp Glu Arg Ser Ser Gly Ile

1 5 10 15

Lys Val Tyr Gly Leu Gln Phe Val Tyr Asp Gly Gln Pro Pro Leu Phe

20 25 30

Ala Glu Phe Asn Leu Asp Ile Ser Pro Gly Ser Arg Cys Leu Leu Val

35 40 45

Gly Ala Asn Gly Ser Gly Lys Thr Thr Leu Leu Lys Ile Leu Ala Gly

50 55 60

Lys His Met Val Gly Gly Arg Asp Val Val Gln Val Leu Asn Gly Ser

65 70 75 80

Ala Phe His Asp Thr His Leu Val Cys Ser Gly Asp Leu Ala Tyr Leu

85 90 95

Gly Gly Ser Trp Ser Lys Thr Val Gly Ser Val Gly Glu Ile Pro Leu

100 105 110

Gln Gly Asp Phe Ser Ala Glu His Met Ile Phe Gly Val Glu Gly Ala

115 120 125

Asp Pro Val Arg Arg Glu Arg Leu Ile Glu Leu Leu Asp Ile Asp Leu

130 135 140

Arg Trp Arg Met His Lys Val Ser Asp Gly Gln Arg Arg Arg Val Gln

145 150 155 160

Ile Cys Met Gly Leu Leu Gln Pro Phe Lys Val Leu Leu Leu Asp Glu

165 170 175

Val Thr Val Asp Leu Asp Val Val Ala Arg Leu Asp Leu Leu Asn Phe

180 185 190

Phe Lys Glu Glu Cys Asp Glu Arg Gly Gly Thr Ile Val Tyr Ala Thr

195 200 205

His Ile Phe Asp Gly Leu Glu Thr Trp Ala Thr His Leu Ala Tyr Ile

210 215 220

Gln Asp Gly Glu Leu Arg Arg Ala Glu Lys Leu Ser Glu Ile Gly Glu

225 230 235 240

Leu Lys Asn Ser Ala Asn Leu Leu Ser Val Val Glu Ser Trp Leu Arg

245 250 255

Ser Glu Ile Lys Leu Glu Lys Lys Lys Pro Val Asn Pro Pro Ala Ala

260 265 270

Gln Ile Gln Lys Thr Ser Pro Phe Gly Thr Ser Pro Phe Val Ser Ser

275 280 285

Arg His Met Ala Tyr Tyr Arg

290 295

<210> 36

<211> 299

<212> PRT

<213> 胡杨(Populus euphratica)

<220>

<221> MISC_FEATURE

<222> (1)..(299)

<223> ABC转运体

<400> 36

Met Ala Glu Lys Ala Ser Gln Ser Ile Glu Lys Gly Gly Glu Asn Glu

1 5 10 15

Lys Ala Asn Ser Ile Ser Ile Trp Gly Met Gln Phe Ala Tyr Ala Gly

20 25 30

Gln His Pro Leu Phe Tyr Asp Phe Asn Leu Asn Ile Ser Pro Gly Ser

35 40 45

Arg Cys Leu Leu Val Gly Ala Asn Gly Ser Gly Lys Thr Thr Leu Leu

50 55 60

Lys Ile Met Ala Gly Lys His Met Val Gly Gly Arg Asp Val Val Arg

65 70 75 80

Val Ile Asn Gly Ser Ala Phe His Asp Thr Gln Leu Val Cys Ser Gly

85 90 95

Asp Leu Ala Tyr Leu Gly Gly Ser Trp Ser Lys Thr Val Gly Ser Ala

100 105 110

Gly Glu Ile Pro Leu Gln Gly Asp Phe Ser Ala Glu His Met Ile Phe

115 120 125

Gly Val Glu Gly Thr Asp Pro Val Arg Arg Glu Lys Leu Ile Asp Leu

130 135 140

Leu Asp Ile Asp Leu Gln Trp Arg Met His Lys Val Ser Asp Gly Gln

145 150 155 160

Arg Arg Arg Val Gln Ile Cys Met Gly Leu Leu His Pro Phe Lys Val

165 170 175

Leu Leu Leu Asp Glu Val Thr Val Asp Leu Asp Val Val Ala Arg Met

180 185 190

Asp Leu Leu Glu Phe Phe Lys Glu Glu Cys Asp Gln Arg Gly Ala Thr

195 200 205

Ile Val Tyr Ala Thr His Ile Phe Asp Gly Leu Glu Thr Trp Ala Thr

210 215 220

His Leu Ala Tyr Ile Gln Asp Gly Glu Leu Arg Arg Ala Glu Lys Leu

225 230 235 240

Thr Glu Val His Glu Leu Lys Ser Ser Ala Asn Leu Leu Ser Val Val

245 250 255

Glu Ser Trp Leu Arg His Glu Thr Lys Ser Glu Lys Lys Lys Pro Thr

260 265 270

Asn Pro Pro Ala Gln Asn Gln Lys Thr Ser Pro Leu Gly Ser Ser Pro

275 280 285

Phe Met Ser Ser Arg His Met Ala Tyr Tyr Arg

290 295

<210> 37

<211> 300

<212> PRT

<213> 木薯(Manihot esculenta)

<220>

<221> MISC_FEATURE

<222> (1)..(300)

<223> ABC转运体

<400> 37

Met Ala Glu Lys Ala Trp Lys Ser Arg Glu Ser Glu Asp Asp Glu Asn

1 5 10 15

Gly Lys Gln Asn Ser Ile Ser Val Arg Gly Met Gln Phe Ala Tyr Glu

20 25 30

Gly Gln His Pro Leu Phe Tyr Asp Phe Asn Leu Asn Ile Pro Pro Gly

35 40 45

Ser Arg Cys Leu Leu Val Gly Ala Asn Gly Ser Gly Lys Thr Thr Leu

50 55 60

Leu Lys Ile Leu Ala Gly Lys His Met Val Gly Gly Arg Asp Val Val

65 70 75 80

Arg Val Leu Asn Arg Ser Ala Phe His Asp Thr Gln Leu Val Cys Gly

85 90 95

Gly Asp Leu Ala Tyr Leu Gly Gly Ser Trp Ser Lys Thr Val Gly Ser

100 105 110

Ala Gly Glu Ile Pro Leu Gln Gly Asp Phe Ser Ala Glu His Met Ile

115 120 125

Phe Gly Val Glu Gly Val Asp Pro Ala Arg Arg Glu Lys Leu Ile Asp

130 135 140

Leu Leu Asp Ile Asp Leu Glu Trp Arg Met His Lys Val Ser Asp Gly

145 150 155 160

Gln Arg Arg Arg Val Gln Ile Cys Met Gly Leu Leu Val Pro Phe Lys

165 170 175

Val Leu Leu Leu Asp Glu Val Thr Val Asp Leu Asp Val Val Ala Arg

180 185 190

Met Asp Leu Leu Glu Phe Phe Arg Glu Glu Cys Glu Gln Arg Gly Ala

195 200 205

Thr Leu Val Tyr Ala Thr His Ile Phe Asp Gly Leu Glu Thr Trp Ala

210 215 220

Thr His Leu Ala Tyr Ile Gln Asp Gly Glu Leu Arg Lys Ser Glu Lys

225 230 235 240

Leu Thr Asp Val His Glu Leu Lys Ser Ser Ala Asn Leu Leu Ser Val

245 250 255

Val Glu Ser Trp Leu Arg Ser Glu Thr Lys His Glu Lys Lys Lys Pro

260 265 270

Thr Asn Pro Pro Ala Gln Asn Gln Lys Thr Ser Pro Phe Gly Thr Ser

275 280 285

Pro Phe Met Ser Ser Arg His Met Ala Tyr Tyr Arg

290 295 300

<210> 38

<211> 294

<212> PRT

<213> 鹰嘴豆(Cicer arietinum)

<220>

<221> MISC_FEATURE

<222> (1)..(294)

<223> ABC转运体

<400> 38

Met Ala Ile Glu Gln Glu Gln Glu Asn Ala Asn Ser Asn Gly Ile Arg

1 5 10 15

Val Gln Gly Met Gln Phe Ser Tyr Asp Gly Leu Gln Gln Pro Pro Leu

20 25 30

Phe Leu Asp Phe Asn Leu Gln Val Ser Pro Gly Ser Arg Cys Leu Leu

35 40 45

Val Gly Ala Asn Gly Ser Gly Lys Thr Thr Leu Leu Lys Ile Leu Ala

50 55 60

Gly Lys His Met Val Gly Gly Lys Asp Val Val Arg Val Leu Asn Cys

65 70 75 80

Ser Ala Phe His Asp Thr Gln Leu Val Cys Ser Gly Asp Leu Ala Tyr

85 90 95

Leu Gly Gly Ser Trp Ser Lys Asn Val Gly Ser Ala Gly Asp Ile Pro

100 105 110

Leu Gln Gly Asp Phe Ser Ala Glu His Met Ile Phe Gly Val Glu Gly

115 120 125

Val Asn Pro Asp Arg Arg Asp Lys Leu Ile Glu Leu Leu Asp Ile Asp

130 135 140

Leu Gln Trp Arg Met His Lys Val Ser Asp Gly Gln Arg Arg Arg Val

145 150 155 160

Gln Ile Cys Leu Gly Leu Leu His Pro Tyr Lys Val Leu Leu Leu Asp

165 170 175

Glu Val Thr Val Asp Leu Asp Val Val Thr Arg Met Asp Leu Leu Glu

180 185 190

Phe Phe Lys Glu Glu Cys Glu Gln Arg Glu Ala Thr Ile Val Tyr Ala

195 200 205

Thr His Ile Phe Asp Gly Leu Glu Thr Trp Ala Thr His Leu Ala Tyr

210 215 220

Ile Gln Glu Gly Glu Leu Arg Arg Ala Glu Lys Leu Ser Asp Val Asn

225 230 235 240

Glu Leu Lys Ser Ser Ile Asn Leu Leu Ser Val Val Glu Ser Trp Leu

245 250 255

Arg Ala Glu Thr Lys Leu Glu Lys Lys Lys Pro Val Leu Asn Thr Ser

260 265 270

Gln Ser Gln Gly Asn Ser Phe Ala Ser Ser Pro Phe Phe Ala Ser Arg

275 280 285

His Met Ala Tyr Tyr Arg

290

<210> 39

<211> 299

<212> PRT

<213> 长蒴黄麻(Corchorus olitorius)

<220>

<221> MISC_FEATURE

<222> (1)..(299)

<223> ABC转运体

<400> 39

Met Ala Glu Gln Thr Phe Cys Lys Ser Arg Glu Gly Asp Glu Asn Ala

1 5 10 15

Glu Ser Lys Ser Ile Arg Val Cys Gly Met Gln Phe Ala Tyr Glu Leu

20 25 30

Gln His Pro Leu Phe Val Asp Phe Asn Leu Glu Ile Ala Pro Gly Ser

35 40 45

Arg Cys Leu Leu Val Gly Ala Asn Gly Ser Gly Lys Thr Thr Leu Leu

50 55 60

Lys Ile Leu Ala Gly Lys His Met Val Gly Gly Arg Asp Val Val Arg

65 70 75 80

Val Leu Asn Arg Ser Ala Phe His Asp Thr Gln Leu Val Cys Gly Gly

85 90 95

Asp Leu Ala Tyr Leu Gly Gly Ser Trp Ser Lys Thr Ile Gly Ser Ala

100 105 110

Gly Glu Val Pro Leu Gln Gly Asp Phe Ser Ala Glu His Met Ile Phe

115 120 125

Gly Val Glu Gly Ile Asp Pro Val Arg Arg Glu Lys Leu Ile Glu Leu

130 135 140

Leu Asp Ile Asp Leu Gln Trp Arg Met His Lys Val Ser Asp Gly Gln

145 150 155 160

Arg Arg Arg Val Gln Ile Cys Met Gly Leu Leu His Pro Phe Gln Val

165 170 175

Leu Leu Leu Asp Glu Val Thr Val Asp Leu Asp Val Val Ala Arg Met

180 185 190

Asp Leu Leu Asp Phe Phe Lys Glu Glu Cys Glu Gln Arg Gly Ala Thr

195 200 205

Val Val Tyr Ala Thr His Ile Phe Asp Gly Leu Glu Thr Trp Ala Thr

210 215 220

His Leu Ala Tyr Ile Gln Asp Gly Glu Leu Lys Lys Ser Glu Lys Ser

225 230 235 240

Thr Glu Ile Asn Glu Leu Lys Ser Ser Glu Asn Leu Leu Ser Val Val

245 250 255

Glu Ala Trp Leu Arg Ser Glu Thr Lys Cys Glu Lys Lys Lys Ala Ile

260 265 270

Asn Asn Ser Ala Gln Val Gln Lys Thr Ser Pro Phe Gly Thr Ser Pro

275 280 285

Phe Met Ser Ser Arg His Met Ala Tyr Tyr Arg

290 295

<210> 40

<211> 300

<212> PRT

<213> 橡胶树(Hevea brasiliensis)

<220>

<221> MISC_FEATURE

<222> (1)..(300)

<223> ABC转运体

<400> 40

Met Ala Glu Lys Arg Trp Lys Ser Arg Glu Ser Glu Glu Asp Glu Asn

1 5 10 15

Asp Lys Gln Tyr Ser Ile Ser Val Cys Gly Met Gln Phe Ala Tyr Glu

20 25 30

Gly Gln Pro Pro Leu Phe Tyr Asp Phe Asn Leu Asn Ile Pro Pro Gly

35 40 45

Ser Arg Cys Leu Leu Val Gly Ala Asn Gly Ser Gly Lys Thr Thr Leu

50 55 60

Leu Lys Ile Leu Ala Gly Lys His Met Val Gly Gly Arg Asp Val Val

65 70 75 80

Arg Val Leu Asn Leu Ser Ala Phe His Asp Thr Gln Leu Val Cys Gly

85 90 95

Gly Asp Leu Ala Tyr Leu Gly Gly Ser Trp Thr Lys Thr Val Gly Ser

100 105 110

Ala Gly Glu Ile Pro Leu Gln Gly Asp Phe Ser Ala Glu His Met Ile

115 120 125

Phe Gly Val Glu Gly Ile Asp Pro Ala Arg Arg Glu Lys Leu Ile Asp

130 135 140

Leu Leu Asp Ile Asp Leu Gln Trp Arg Met His Lys Val Ser Asp Gly

145 150 155 160

Gln Arg Arg Arg Val Gln Ile Cys Met Gly Leu Leu Val Pro Phe Lys

165 170 175

Val Leu Leu Leu Asp Glu Val Thr Val Asp Leu Asp Val Val Ala Arg

180 185 190

Leu Asp Leu Leu Glu Phe Phe Lys Glu Glu Cys Glu Gln Arg Gly Ala

195 200 205

Thr Leu Val Tyr Ala Thr His Ile Phe Asp Gly Leu Glu Thr Trp Ala

210 215 220

Thr His Leu Ala Tyr Ile Gln Asp Gly Glu Leu Arg Arg Ser Glu Lys

225 230 235 240

Leu Thr Glu Val His Glu Leu Lys Ser Ser Ala Asn Leu Leu Ser Val

245 250 255

Val Glu Ser Trp Leu Arg Ser Glu Thr Arg His Glu Lys Lys Lys Pro

260 265 270

Thr Asn Pro Pro Ala Gln Asn Gln Lys Thr Ser Pro Phe Gly Thr Ser

275 280 285

Pro Phe Met Ser Ser Arg His Met Ala Tyr Tyr Arg

290 295 300

<210> 41

<211> 298

<212> PRT

<213> 胡杨(Populus euphratica)

<220>

<221> MISC_FEATURE

<222> (1)..(298)

<223> ABC转运体

<400> 41

Met Ala Glu Lys Ala Ser Gln Ser Asp Gly Gly Gly Glu Asn Glu Lys

1 5 10 15

Glu Asn Ser Ile Ser Ile Cys Gly Met Gln Tyr Ala Tyr Pro Gly Gln

20 25 30

His Pro Leu Phe Tyr Glu Phe Asn Leu Asn Ile Ser Pro Gly Ser Arg

35 40 45

Cys Leu Leu Val Gly Ala Asn Gly Ser Gly Lys Thr Thr Leu Leu Lys

50 55 60

Ile Met Ala Gly Lys His Met Val Gly Gly Arg Asp Val Val Arg Val

65 70 75 80

Ile Asn Gly Ser Ala Phe His Asp Thr Gln Leu Val Cys Ser Gly Asp

85 90 95

Leu Ala Tyr Leu Gly Gly Ser Trp Ser Lys Thr Val Gly Ser Ala Gly

100 105 110

Glu Ile Pro Leu Gln Gly Asp Phe Ser Ala Glu His Met Ile Phe Gly

115 120 125

Val Glu Gly Thr Asp Pro Val Arg Arg Glu Lys Leu Ile Glu Leu Leu

130 135 140

Asp Ile Asp Leu Lys Trp Arg Met His Lys Val Ser Asp Gly Gln Arg

145 150 155 160

Arg Arg Val Gln Ile Cys Met Gly Leu Leu His Pro Phe Lys Val Leu

165 170 175

Leu Leu Asp Glu Val Thr Val Asp Leu Asp Val Val Ala Arg Met Asp

180 185 190

Leu Leu Glu Phe Phe Lys Glu Glu Cys Asp Gln Arg Gly Ala Thr Ile

195 200 205

Val Tyr Ala Thr His Ile Phe Asp Gly Leu Glu Thr Trp Ala Thr His

210 215 220

Leu Ala Tyr Ile Gln Asp Gly Glu Leu Lys Arg Ala Lys Lys Leu Thr

225 230 235 240

Glu Ile His Glu Leu Lys Asn Ser Ala Thr Leu Leu Ser Val Val Glu

245 250 255

Ser Trp Leu Arg Ser Glu Thr Lys Asn Glu Lys Lys Lys Pro Ala Asn

260 265 270

Pro Pro Ala Gln Asn Gln Lys Thr Ser Pro Phe Gly Ser Ser Pro Phe

275 280 285

Met Ser Ser Arg His Met Ala Phe Tyr Arg

290 295

<210> 42

<211> 301

<212> PRT

<213> 梅(Prunus mume)

<220>

<221> MISC_FEATURE

<222> (1)..(301)

<223> ABC转运体

<400> 42

Met Ala Gly Arg Val Ser Glu Thr Ser Asn Pro Arg Ala Glu Gly Glu

1 5 10 15

Gly Glu Thr Ser Ser Gly Ile Arg Val His Gly Met Gln Phe Ser Tyr

20 25 30

Asp Ala Gln Pro Pro Leu Phe Cys Asp Phe Asn Leu Asn Ile Ala Pro

35 40 45

Gly Ser Arg Cys Leu Leu Val Gly Ala Asn Gly Ser Gly Lys Thr Thr

50 55 60

Leu Leu Lys Ile Leu Ala Gly Lys Gln Met Val Gly Gly Arg Asp Val

65 70 75 80

Val Arg Val Leu Asn Cys Ser Ala Phe His Asp Thr His Leu Val Cys

85 90 95

Ser Gly Asp Leu Ala Tyr Leu Gly Gly Ser Trp Ser Lys Ser Val Gly

100 105 110

Ser Ala Gly Glu Leu Pro Leu Gln Gly Asp Phe Ser Ala Glu His Met

115 120 125

Ile Phe Gly Val Glu Gly Thr Asp Pro Val Arg Arg Asp Lys Leu Ile

130 135 140

Glu Leu Leu Asp Ile Asn Leu Gln Trp Arg Met His Lys Val Ser Asp

145 150 155 160

Gly Gln Arg Arg Arg Val Gln Ile Cys Met Gly Leu Leu His Pro Phe

165 170 175

Lys Val Leu Leu Leu Asp Glu Val Thr Val Asp Leu Asp Val Val Ala

180 185 190

Arg Leu Asp Leu Leu Asp Phe Phe Lys Glu Glu Cys Glu Gln Arg Gly

195 200 205

Ala Thr Ile Val Tyr Ala Thr His Ile Phe Asp Gly Leu Glu Thr Trp

210 215 220

Ala Thr His Leu Ala Tyr Ile Gln Asp Gly Glu Leu Lys Arg Ala Glu

225 230 235 240

Lys Leu Ser Glu Val Asn Glu Leu Lys Ser Ser Ala Asn Leu Leu Ser

245 250 255

Val Val Glu Ser Trp Leu Arg Ala Glu Thr Lys His Glu Lys Lys Arg

260 265 270

Ser Thr Asn Pro Pro Ala Gln Thr Gln Lys Thr Ser Pro Phe Ala Thr

275 280 285

Ser Pro Phe Met Ser Ser Arg His Met Ala Tyr Tyr Arg

290 295 300

<210> 43

<211> 300

<212> PRT

<213> 雷蒙德氏棉(Gossypium raimondii)

<220>

<221> MISC_FEATURE

<222> (1)..(300)

<223> ABC转运体

<400> 43

Met Ala Glu Gln Ala Cys Cys Arg Ser Gly Glu Gly Lys Glu Asn Gly

1 5 10 15

Gly Asp Ser Gln Gly Ile Thr Val Cys Gly Met Gln Phe Ala Tyr Glu

20 25 30

Leu Gln His Pro Leu Phe Phe Asp Phe Asn Leu Asp Ile Ala Arg Gly

35 40 45

Ser Arg Cys Leu Leu Val Gly Ala Asn Gly Ser Gly Lys Thr Thr Leu

50 55 60

Leu Lys Ile Leu Ala Gly Lys His Met Val Gly Gly Arg Glu Val Val

65 70 75 80

Arg Val Leu Asn Arg Ser Ala Phe His Asp Thr Gln Leu Val Cys Gly

85 90 95

Gly Asp Leu Ala Tyr Leu Gly Gly Ser Trp Ser Lys Thr Ile Gly Ser

100 105 110

Ala Gly Glu Val Pro Leu Gln Gly Asp Phe Ser Ala Glu His Met Ile

115 120 125

Phe Gly Val Glu Gly Ile Asp Pro Val Arg Arg Asp Lys Leu Ile Glu

130 135 140

Leu Leu Asp Ile Asp Leu Gln Trp Arg Met His Lys Val Ser Asp Gly

145 150 155 160

Gln Arg Arg Arg Val Gln Ile Cys Met Gly Leu Leu His Pro Phe Gln

165 170 175

Val Leu Leu Leu Asp Glu Val Thr Val Asp Leu Asp Val Val Ala Arg

180 185 190

Met Asp Leu Leu Asp Phe Phe Lys Glu Glu Cys Glu Gln Arg Gly Ala

195 200 205

Thr Val Val Tyr Ala Thr His Ile Phe Asp Gly Leu Glu Thr Trp Ala

210 215 220

Thr His Leu Ala Tyr Ile Gln Asp Gly Gln Leu Lys Arg Ser Glu Lys

225 230 235 240

Leu Thr Glu Ile Asn Glu Leu Lys Ser Ser Glu Asn Leu Leu Ser Val

245 250 255

Val Glu Ala Trp Leu Arg Ser Glu Thr Lys Cys Glu Lys Lys Lys Pro

260 265 270

Ser Asn Ser Pro Ala Gln Val Gln Lys Ser Ser Pro Phe Gly Thr Ser

275 280 285

Pro Phe Met Ser Ser Arg His Met Ala Tyr Tyr Arg

290 295 300

<210> 44

<211> 300

<212> PRT

<213> 枣(Ziziphus jujube)

<220>

<221> MISC_FEATURE

<222> (1)..(300)

<223> ABC转运体

<400> 44

Met Glu Asp Arg Gly Ser Leu Lys Pro Glu Glu Glu Gly Ser Lys Tyr

1 5 10 15

Glu Ser Ser Ser Ser Ile Lys Val Tyr Gly Met Gln Phe Ser Tyr Asp

20 25 30

Gly Gln Thr Pro Leu Phe Ala Asp Phe Asn Leu Asp Ile Ser Pro Gly

35 40 45

Ser Arg Cys Leu Leu Val Gly Ala Asn Gly Ser Gly Lys Thr Thr Leu

50 55 60

Leu Lys Ile Leu Ala Gly Lys His Met Val Gly Glu Arg Asp Val Val

65 70 75 80

Arg Val Leu Asn Tyr Ser Ala Phe His Asp Thr His Leu Val Cys Ser

85 90 95

Gly Asp Leu Ala Tyr Leu Gly Gly Ser Trp Ser Lys Thr Val Gly Ser

100 105 110

Ala Gly Glu Ile Pro Leu Gln Gly Asp Phe Ser Ala Glu His Met Ile

115 120 125

Phe Gly Val Glu Gly Ala Asp Pro Val Arg Arg Asp Lys Leu Ile Glu

130 135 140

Leu Leu Asp Ile Asp Leu Gln Trp Arg Met His Lys Val Ser Asp Gly

145 150 155 160

Gln Arg Arg Arg Val Gln Ile Cys Met Gly Leu Leu His Pro Phe Lys

165 170 175

Val Leu Leu Leu Asp Glu Val Thr Val Asp Leu Asp Val Val Ala Arg

180 185 190

Met Asp Leu Leu Glu Phe Phe Lys Glu Glu Cys Asp Gln Arg Gly Ala

195 200 205

Thr Ile Val Tyr Ala Thr His Ile Phe Asp Gly Leu Glu Thr Trp Ala

210 215 220

Thr His Leu Ala Tyr Ile Gln Asp Gly Asp Leu Arg Arg Ala Glu Lys

225 230 235 240

Leu Ser Glu Val Asn Glu Leu Lys Asn Ser Ala Asn Leu Leu Ser Val

245 250 255

Val Glu Ser Trp Leu Arg Ser Glu Ile Lys His Glu Lys Lys Lys Pro

260 265 270

Ile Asn Pro Pro Ser Gln Ile Arg Lys Thr Ser Pro Phe Gly Thr Ser

275 280 285

Pro Phe Val Ser Ser Arg His Met Ala Tyr Tyr Arg

290 295 300

<210> 45

<211> 294

<212> PRT

<213> 克莱门柚(Citrus clementina)

<220>

<221> MISC_FEATURE

<222> (1)..(294)

<223> ABC转运体

<400> 45

Met Ala Asp Gly Met Asn Glu Asn Glu Lys Leu Asn Ser Ile Lys Val

1 5 10 15

Cys Gly Met Gln Phe Ser Tyr Glu Gly Asn Asp Lys Pro Pro Leu Phe

20 25 30

Tyr Asp Phe Asn Leu Asp Ile Ser Pro Gly Ser Arg Cys Leu Leu Val

35 40 45

Gly Ala Asn Gly Ser Gly Lys Thr Thr Leu Leu Lys Ile Leu Ala Gly

50 55 60

Lys His Met Val Gly Gly Arg Asp Val Val Gln Val Leu Asn Arg Ser

65 70 75 80

Ser Phe His Asp Thr Gln Leu Val Cys Ser Gly Asp Leu Ser Tyr Leu

85 90 95

Gly Gly Ser Trp Ser Lys Thr Val Gly Ser Ala Gly Glu Ile Pro Leu

100 105 110

Gln Gly Asp Phe Ser Ala Glu His Met Ile Phe Gly Val Glu Gly Ser

115 120 125

Asp Pro Val Arg Arg Glu Arg Leu Ile Glu Leu Leu Asp Ile Asp Leu

130 135 140

Gln Trp Arg Met His Lys Val Ser Asp Gly Gln Arg Arg Arg Val Gln

145 150 155 160

Ile Cys Met Gly Leu Leu His Pro Phe Lys Val Leu Leu Leu Asp Glu

165 170 175

Ile Thr Val Asp Leu Asp Val Val Ala Arg Met Asp Leu Leu Asp Phe

180 185 190

Phe Lys Asp Glu Cys Glu Gln Arg Gly Ala Thr Ile Val Tyr Ala Thr

195 200 205

His Ile Phe Asp Gly Leu Glu Thr Trp Ala Thr His Leu Ala Tyr Ile

210 215 220

Gln Asp Gly Glu Leu Arg Arg Ala Glu Lys Leu Ala Glu Leu Asp Glu

225 230 235 240

Leu Arg Asn Ser Thr Asn Leu Leu Ser Val Val Glu Ser Trp Leu Arg

245 250 255

Ser Glu Thr Lys Leu Glu Lys Lys Arg Pro Val Asp Pro Pro Lys Gln

260 265 270

Val Gln Lys Thr Ser Pro Phe Gly Ser Ser Pro Phe Met Ser Ser Arg

275 280 285

His Met Ala Tyr Tyr Arg

290

<210> 46

<211> 336

<212> PRT

<213> 牵牛(Ipomoea nil)

<220>

<221> MISC_FEATURE

<222> (1)..(336)

<223> ABC转运体

<400> 46

Met Phe Ser Arg Arg Val Asn Ile Gln Ser Leu Glu Glu Val Val Glu

1 5 10 15

Val Val Arg Glu Glu Thr Leu Leu Arg Arg Leu Leu Tyr Asn Thr Pro

20 25 30

Pro Glu Thr Pro Leu Ile Glu Tyr Arg Arg Pro Arg Glu Lys Glu Thr

35 40 45

Lys Met Ala Ala Glu Ser Asn Ser Ile Gln Val Ser Gly Met Gln Phe

50 55 60

Gly Tyr Asp Phe Gln Thr Pro Leu Phe Phe Asp Phe Ser Leu Lys Val

65 70 75 80

Ser Pro Gly Ser Arg Cys Leu Leu Val Gly Ala Asn Gly Ser Gly Lys

85 90 95

Thr Thr Leu Leu Arg Ile Leu Ala Gly Lys His Leu Val Gly Gly Lys

100 105 110

Asp Val Val Arg Val Leu Asn Phe Ser Ala Phe His Asp Thr His Leu

115 120 125

Val Cys Ser Gly Asp Leu Ala Tyr Leu Gly Glu Ser Trp Ser Lys Asn

130 135 140

Val Gly Ser Ala Gly Glu Ile Pro Leu Gln Gly Asp Phe Ser Ala Glu

145 150 155 160

His Met Ile Phe Gly Val Asp Gly Val Asp Pro Ile Arg Arg Glu Lys

165 170 175

Leu Ile Glu Leu Leu Asp Ile Asp Leu Gln Trp Arg Met His Lys Val

180 185 190

Ser Asp Gly Gln Arg Arg Arg Val Gln Ile Cys Met Gly Leu Leu His

195 200 205

Pro Tyr Lys Val Leu Leu Leu Asp Glu Val Thr Val Asp Leu Asp Val

210 215 220

Val Ala Arg Met Asp Leu Leu Asp Phe Phe Lys Glu Glu Cys Glu Gln

225 230 235 240

Arg Gly Ala Thr Val Val Tyr Ala Thr His Ile Phe Asp Gly Leu Glu

245 250 255

Thr Trp Ala Thr Asp Leu Val Tyr Val Gln Asp Gly Val Leu Lys Arg

260 265 270

Ser Glu Lys Leu Ser Glu Leu Ala Glu Leu Lys Thr Lys Pro Asn Leu

275 280 285

Leu Ser Val Val Glu Thr Trp Leu Arg Ser Glu Thr Pro Ile Glu Lys

290 295 300

Lys Lys Ala Ser Lys Asn Pro Ser Ala Val Lys Thr Thr Ser Ser Pro

305 310 315 320

Phe Asp Ala Ser Pro Phe Arg Ser Ser Arg His Met Ala Tyr Tyr Arg

325 330 335

<210> 47

<211> 301

<212> PRT

<213> 桃(Prunus persica)

<220>

<221> MISC_FEATURE

<222> (1)..(301)

<223> ABC转运体

<400> 47

Met Ala Gly Arg Val Ser Glu Thr Ser Asn Pro Arg Ala Glu Gly Glu

1 5 10 15

Gly Glu Thr Ser Ser Gly Ile Arg Val His Gly Met Gln Phe Ser Tyr

20 25 30

Asp Ala Gln Pro Pro Leu Phe Cys Asp Phe Asn Leu Asn Ile Ala Pro

35 40 45

Gly Ser Arg Cys Leu Leu Val Gly Ala Asn Gly Ser Gly Lys Thr Thr

50 55 60

Leu Leu Lys Ile Leu Ala Gly Lys Gln Met Val Gly Gly Arg Asp Val

65 70 75 80

Val Arg Val Leu Asn Cys Ser Ala Phe His Asp Thr His Leu Val Cys

85 90 95

Ser Gly Asp Leu Ala Tyr Leu Gly Gly Ser Trp Ser Lys Ser Val Gly

100 105 110

Ser Ala Gly Glu Leu Pro Leu Gln Gly Asp Phe Ser Ala Glu His Met

115 120 125

Ile Phe Gly Val Glu Gly Thr Asp Pro Val Arg Arg Asp Lys Leu Ile

130 135 140

Glu Leu Leu Asp Ile Asn Leu Gln Trp Arg Met His Lys Val Ser Asp

145 150 155 160

Gly Gln Arg Arg Arg Val Gln Ile Cys Met Gly Leu Leu His Pro Phe

165 170 175

Lys Val Leu Leu Leu Asp Glu Val Thr Val Asp Leu Asp Val Val Ala

180 185 190

Arg Leu Asp Leu Leu Asp Phe Phe Lys Glu Glu Cys Glu Gln Arg Arg

195 200 205

Ala Thr Ile Val Tyr Ala Thr His Ile Phe Asp Gly Leu Glu Thr Trp

210 215 220

Ala Thr His Leu Ala Tyr Ile Gln Asp Gly Glu Leu Lys Arg Ala Glu

225 230 235 240

Lys Leu Ser Glu Val Asn Glu Leu Lys Ser Ser Ala Asn Leu Leu Ser

245 250 255

Val Val Glu Ser Trp Leu Arg Ala Glu Thr Lys His Glu Lys Lys Arg

260 265 270

Ser Thr Asn Pro Pro Ala Gln Thr Gln Lys Thr Ser Ala Phe Ala Thr

275 280 285

Ser Pro Phe Met Ser Ser Arg His Met Ala Tyr Tyr Arg

290 295 300

<210> 48

<211> 300

<212> PRT

<213> 陆地棉(Gossypium hirsutum)

<220>

<221> MISC_FEATURE

<222> (1)..(300)

<223> ABC转运体

<400> 48

Met Ala Glu Gln Ala Cys Cys Arg Ser Gly Glu Gly Lys Glu Asn Gly

1 5 10 15

Gly Asp Leu Gln Gly Ile Thr Val Cys Gly Met Gln Phe Ala Tyr Glu

20 25 30

Leu Gln His Pro Leu Phe Phe Asp Phe Asn Leu Asp Ile Ala Arg Gly

35 40 45

Ser Arg Cys Leu Leu Val Gly Ala Asn Gly Ser Gly Lys Thr Thr Leu

50 55 60

Leu Lys Ile Leu Ala Gly Lys His Met Val Gly Gly Arg Asp Val Val

65 70 75 80

Arg Val Leu Asn Arg Ser Ala Phe His Asp Thr Gln Leu Val Cys Gly

85 90 95

Gly Asp Leu Ala Tyr Leu Gly Gly Ser Trp Ser Lys Thr Ile Gly Ser

100 105 110

Ala Gly Glu Val Pro Leu Gln Gly Asp Phe Ser Ala Glu His Met Ile

115 120 125

Phe Gly Val Glu Gly Ile Asp Pro Val Arg Arg Asp Lys Leu Ile Glu

130 135 140

Leu Leu Asp Ile Asp Leu Gln Trp Arg Met His Lys Val Ser Asp Gly

145 150 155 160

Gln Arg Arg Arg Val Gln Ile Cys Met Gly Leu Leu His Pro Phe Gln

165 170 175

Val Leu Leu Leu Asp Glu Val Thr Val Asp Leu Asp Val Val Ala Arg

180 185 190

Met Asp Leu Leu Asp Phe Phe Lys Glu Glu Cys Glu Gln Arg Gly Ala

195 200 205

Thr Val Val Tyr Ala Thr His Ile Phe Asp Gly Leu Glu Thr Trp Ala

210 215 220

Thr His Leu Ala Tyr Ile Gln Asp Gly Gln Leu Lys Arg Ser Glu Lys

225 230 235 240

Leu Thr Glu Ile Asn Glu Leu Lys Ser Ser Glu Asn Leu Leu Ser Val

245 250 255

Val Glu Ala Trp Leu Arg Ser Glu Thr Lys Cys Glu Lys Lys Lys Ser

260 265 270

Ser Asn Ser Pro Ala Gln Val Gln Lys Ser Ser Pro Phe Gly Thr Ser

275 280 285

Pro Phe Met Ser Ser Arg His Met Ala Tyr Tyr Arg

290 295 300

<210> 49

<211> 274

<212> PRT

<213> 毛果杨(Populus trichocarpa)

<220>

<221> MISC_FEATURE

<222> (1)..(274)

<223> ABC转运体

<400> 49

Met Gln Phe Ala Tyr Ala Gly Gln His Pro Leu Phe Tyr Asp Phe Asn

1 5 10 15

Leu Asn Ile Ser Pro Gly Ser Arg Cys Leu Leu Val Gly Ala Asn Gly

20 25 30

Ser Gly Lys Thr Thr Leu Leu Lys Ile Met Ala Gly Lys His Met Val

35 40 45

Gly Gly Lys Asp Val Val Arg Val Ile Asn Gly Ser Ala Phe His Asp

50 55 60

Thr Gln Leu Val Cys Ser Gly Asp Leu Ala Tyr Leu Gly Gly Ser Trp

65 70 75 80

Ser Lys Thr Val Gly Ser Ala Gly Glu Ile Pro Leu Gln Gly Asp Phe

85 90 95

Ser Ala Glu His Met Ile Phe Gly Val Glu Gly Thr Asp Pro Val Arg

100 105 110

Arg Glu Lys Leu Ile Asp Leu Leu Asp Ile Asp Leu Gln Trp Arg Met

115 120 125

His Lys Val Ser Asp Gly Gln Arg Arg Arg Val Gln Ile Cys Met Gly

130 135 140

Leu Leu His Pro Phe Lys Val Leu Leu Leu Asp Glu Val Thr Val Asp

145 150 155 160

Leu Asp Val Val Ala Arg Met Asp Leu Leu Glu Phe Phe Lys Glu Glu

165 170 175

Cys Asp Gln Arg Gly Ala Thr Ile Val Tyr Ala Thr His Ile Phe Asp

180 185 190

Gly Leu Glu Thr Trp Ala Thr His Leu Ala Tyr Ile Gln Asp Gly Glu

195 200 205

Leu Arg Arg Ala Glu Lys Leu Thr Glu Val His Glu Leu Lys Ser Ser

210 215 220

Ala Asn Leu Leu Ser Val Val Glu Ser Trp Leu Arg His Glu Thr Lys

225 230 235 240

Ser Glu Lys Lys Lys Pro Thr Asn Pro Pro Ala Gln Asn Gln Lys Thr

245 250 255

Ser Pro Leu Gly Ser Ser Pro Phe Met Ser Ser Arg His Met Ala Tyr

260 265 270

Tyr Arg

<210> 50

<211> 287

<212> PRT

<213> 白梨(Pyrus x bretschneideri)

<220>

<221> MISC_FEATURE

<222> (1)..(287)

<223> ABC转运体

<400> 50

Met Ala Glu Glu Ala Ser Ser Gly Ile Arg Val Gln Gly Met Gln Phe

1 5 10 15

Ala Tyr Asp Ala Gln Pro Pro Leu Phe Cys Asp Phe Asn Leu Glu Ile

20 25 30

Thr Pro Gly Ser Arg Cys Leu Leu Ile Gly Ala Asn Gly Ser Gly Lys

35 40 45

Thr Thr Leu Leu Lys Ile Leu Ala Gly Lys Gln Met Val Gly Gly Arg

50 55 60

Asp Val Val Arg Val Leu Asn Cys Ser Ala Phe His Asp Thr His Leu

65 70 75 80

Val Cys Ser Gly Asp Leu Ala Tyr Leu Gly Gly Ser Trp Thr Lys Thr

85 90 95

Val Gly Ser Ala Gly Glu Leu Pro Leu Gln Gly Asp Phe Ser Ala Glu

100 105 110

His Met Ile Phe Gly Val Glu Gly Thr Asp Pro Val Arg Arg Asp Lys

115 120 125

Leu Ile Glu Leu Leu Asp Ile Asp Leu Arg Trp Arg Met His Lys Val

130 135 140

Ser Asp Gly Gln Arg Arg Arg Val Gln Ile Cys Met Gly Leu Leu His

145 150 155 160

Pro Phe Lys Val Leu Leu Leu Asp Glu Val Thr Val Asp Leu Asp Val

165 170 175

Val Ala Arg Met Asp Leu Leu Asp Phe Phe Lys Glu Glu Cys Glu Gln

180 185 190

Arg Gly Ala Thr Ile Val Tyr Ala Thr His Ile Phe Asp Gly Leu Glu

195 200 205

Thr Trp Ala Thr His Leu Ala Tyr Ile Gln Asp Gly Glu Leu Lys Arg

210 215 220

Ala Glu Lys Leu Ser Glu Val Asn Glu Leu Lys Ser Ser Val Asn Leu

225 230 235 240

Leu Ser Val Val Glu Ser Trp Leu Arg Ala Glu Thr Lys Ser Asp Lys

245 250 255

Lys Lys Ser Ser Asn Pro Pro Ala Gln Met Lys Lys Ala Ser Pro Phe

260 265 270

Ala Ser Ser Pro Phe Thr Ser Ser Arg His Met Ala Tyr Tyr Arg

275 280 285

<210> 51

<211> 286

<212> PRT

<213> 刺苞菜蓟(Cynara cardunculus)

<220>

<221> MISC_FEATURE

<222> (1)..(286)

<223> ABC转运体

<400> 51

Met Glu Val Glu Ser Ser Ser Ile Lys Val Asn Gly Met Gln Phe Gly

1 5 10 15

Tyr Ser Gly Glu Asn Pro Ile Phe Val Asp Phe Asn Leu Lys Ile Ser

20 25 30

Pro Arg Ser Arg Cys Leu Leu Val Gly Ala Asn Gly Ser Gly Lys Thr

35 40 45

Thr Leu Leu Lys Ile Leu Ala Gly Lys His Met Val Gly Gly Arg Asn

50 55 60

Val Val Arg Val Leu Asp Phe Ser Ser Phe His Asp Thr His Leu Val

65 70 75 80

Cys Ser Gly Asp Leu Ser Tyr Leu Gly Gly Ser Trp Thr Lys Thr Ile

85 90 95

Gly Ser Ala Gly Glu Val Pro Leu Gln Gly Asp Phe Ser Ala Glu His

100 105 110

Met Ile Phe Gly Val Glu Gly Ile Asp Pro Val Arg Arg Glu Lys Leu

115 120 125

Ile Asp Leu Leu Asp Ile Asp Leu Gln Trp Arg Met His Lys Val Ser

130 135 140

Asp Gly Gln Arg Arg Arg Val Gln Ile Cys Leu Gly Leu Leu His Pro

145 150 155 160

Phe Gln Val Leu Leu Leu Asp Glu Val Thr Val Asp Leu Asp Val Val

165 170 175

Ala Arg Met Asp Leu Leu Glu Phe Phe Lys Glu Glu Cys Glu Gln Arg

180 185 190

Gly Ala Thr Ile Val Tyr Ala Thr His Ile Phe Asp Gly Leu Glu Ser

195 200 205

Trp Ala Thr Asp Leu Ala Tyr Ile Gln Asp Gly Glu Leu Lys Arg Tyr

210 215 220

Glu Lys Leu Ala Asp Leu Pro Glu Met Lys Asn Ser Ser Asn Leu Leu

225 230 235 240

Ser Val Val Glu Ser Trp Leu Arg Ala Glu Thr Lys Asn Pro Lys Lys

245 250 255

Lys Pro Val Asn Pro Ser Pro Leu Ile Pro Lys Thr Ser Pro Phe Asp

260 265 270

Ser Ser Pro Phe Arg Ser Ser Arg His Met Ala Tyr Tyr Arg

275 280 285

<210> 52

<211> 286

<212> PRT

<213> 芝麻(Sesamum indicum)

<220>

<221> MISC_FEATURE

<222> (1)..(286)

<223> ABC转运体

<400> 52

Met Gly Glu Glu Ser His Ser Ile Gln Val Ser Gly Met Gln Phe Ser

1 5 10 15

Tyr Asp Ala Asn Ser Pro Leu Phe Phe Asp Phe Ser Leu Asn Ile Tyr

20 25 30

Pro Gly Ser Arg Cys Leu Leu Val Gly Ala Asn Gly Ser Gly Lys Thr

35 40 45

Thr Leu Leu Arg Ile Leu Ala Gly Lys His Met Val Gly Gly Arg Asp

50 55 60

Val Val Arg Val Leu Asn Cys Ser Ala Phe His Asp Thr Ser Leu Val

65 70 75 80

Cys Ser Gly Asp Leu Ala Tyr Leu Gly Glu Ser Trp Ser Lys Thr Val

85 90 95

Gly Ser Ala Gly Glu Leu Pro Leu Gln Gly Asp Phe Ser Ala Glu His

100 105 110

Met Ile Phe Gly Val Glu Gly Val Asp Pro Val Arg Arg Asp Lys Leu

115 120 125

Ile Glu Leu Leu Asp Ile Asp Leu His Trp Arg Met His Lys Val Ser

130 135 140

Asp Gly Gln Arg Arg Arg Val Gln Ile Cys Met Gly Leu Leu His Pro

145 150 155 160

Tyr Lys Val Leu Leu Leu Asp Glu Val Thr Val Asp Leu Asp Val Val

165 170 175

Ala Arg Met Asp Leu Leu Glu Phe Phe Lys Glu Glu Cys Glu Gln Arg

180 185 190

Arg Ala Thr Ile Val Tyr Ala Thr His Ile Phe Asp Gly Leu Glu Thr

195 200 205

Trp Ala Thr Asp Leu Val Tyr Val Gln Glu Gly Val Leu Lys Arg Ser

210 215 220

Asp Lys Leu Ala Asp Leu Lys Glu Leu Lys Asn Asn Met Asn Leu Leu

225 230 235 240

Ser Val Val Glu Ser Trp Leu Arg Thr Glu Thr Lys Ile Glu Lys Lys

245 250 255

Lys Pro Val Asn Ser Pro Ser Pro Leu Gln Lys Ser Ser Pro Phe Asp

260 265 270

Asn Ser Pro Phe Arg Ser Ser Arg His Met Ala Tyr Tyr Arg

275 280 285

<210> 53

<211> 300

<212> PRT

<213> 陆地棉(Gossypium hirsutum)

<220>

<221> MISC_FEATURE

<222> (1)..(300)

<223> ABC转运体

<400> 53

Met Ala Glu Gln Ala Cys Cys Arg Ser Gly Glu Gly Lys Glu Asn Gly

1 5 10 15

Gly Asp Ser Gln Gly Ile Thr Val Cys Asp Met Gln Phe Ala Tyr Glu

20 25 30

Leu Gln His Pro Leu Phe Phe Asp Phe Asn Leu Asp Ile Ala Arg Gly

35 40 45

Ser Arg Cys Leu Leu Val Gly Ala Asn Gly Ser Gly Lys Thr Thr Leu

50 55 60

Leu Lys Ile Leu Ala Gly Lys His Met Val Gly Gly Arg Glu Val Val

65 70 75 80

Arg Val Leu Asn Arg Ser Ala Phe His Asp Thr Gln Leu Val Cys Gly

85 90 95

Gly Asp Leu Ala Tyr Leu Gly Gly Ser Trp Ser Lys Thr Ile Gly Ser

100 105 110

Ala Gly Glu Val Pro Leu Gln Gly Asp Phe Ser Ala Glu His Met Ile

115 120 125

Phe Gly Val Glu Gly Ile Asp Pro Val Arg Arg Asp Lys Leu Ile Glu

130 135 140

Leu Leu Asp Ile Asp Leu Gln Trp Arg Met His Lys Val Ser Asp Gly

145 150 155 160

Gln Arg Arg Arg Val Gln Ile Cys Met Gly Leu Leu Gln Pro Phe Gln

165 170 175

Val Leu Leu Leu Asp Glu Val Thr Val Asp Leu Asp Val Val Ala Arg

180 185 190

Met Asp Leu Leu Asp Phe Phe Lys Glu Glu Cys Glu Gln Arg Gly Ala

195 200 205

Thr Val Val Tyr Ala Thr His Ile Phe Asp Gly Leu Glu Thr Trp Ala

210 215 220

Thr His Leu Ala Tyr Ile Gln Asp Gly Gln Leu Lys Arg Ser Ala Lys

225 230 235 240

Leu Thr Glu Ile Asn Glu Leu Lys Ser Ser Glu Asn Leu Leu Ser Val

245 250 255

Val Glu Ala Trp Leu Arg Ser Glu Thr Lys Cys Glu Lys Lys Lys Pro

260 265 270

Ser Asn Ser Pro Ala Gln Val Gln Lys Ser Ser Pro Phe Gly Thr Ser

275 280 285

Pro Phe Met Ser Ser Arg His Met Ala Tyr Tyr Arg

290 295 300

<210> 54

<211> 292

<212> PRT

<213> 甜菜(Beta vulgaris)

<220>

<221> MISC_FEATURE

<222> (1)..(292)

<223> ABC转运体

<400> 54

Met Val Gln Glu Met Lys Asn Val Glu Ser Ser Thr Ser Ile Lys Val

1 5 10 15

Gln Ala Met Gln Phe Ala Tyr Pro Gly Gln Pro Pro Ile Phe Ala Asp

20 25 30

Phe Ser Leu Asp Ile Ser Ala Gly Ser Arg Cys Leu Leu Val Gly Ala

35 40 45

Asn Gly Ser Gly Lys Thr Thr Leu Leu Lys Ile Leu Ala Gly Lys His

50 55 60

Met Val Gly Gly Arg Asp Val Val Gln Val Leu Asn Ser Ser Ala Phe

65 70 75 80

His Asp Thr Arg Leu Val Cys Ser Gly Asp Leu Ala Tyr Leu Gly Gly

85 90 95

Ser Trp Ser Lys Thr Ile Gly Ser Ala Gly Glu Val Pro Leu Gln Gly

100 105 110

Asp Phe Ser Ala Glu Gln Met Ile Phe Gly Val Glu Gly Ser Asp Pro

115 120 125

Asp Arg Arg Ala Lys Leu Ile Glu Leu Leu Asp Ile Asp Leu Gln Trp

130 135 140

Arg Met His Lys Val Ser Asp Gly Gln Arg Arg Arg Val Gln Ile Cys

145 150 155 160

Met Gly Leu Leu His Pro Phe Lys Ile Leu Leu Leu Asp Glu Val Thr

165 170 175

Val Asp Leu Asp Val Val Ala Arg Met Asp Leu Leu Glu Phe Leu Lys

180 185 190

Glu Glu Cys Asp Gln Arg Gly Ala Thr Ile Val Tyr Ala Thr His Ile

195 200 205

Phe Asp Gly Leu Glu Thr Trp Ala Thr His Leu Ala Tyr Ile Gln Glu

210 215 220

Gly Asp Leu Arg Lys Ile Glu Lys Leu Ser Glu Leu His Glu Leu Lys

225 230 235 240

Thr Ser Asn Asn Leu Leu Ser Val Val Glu Ser Trp Leu Arg Ser Glu

245 250 255

Thr Lys Ile Glu Lys Lys Lys Pro Ala Thr Thr Ala Thr Gln Asn Gln

260 265 270

Lys Ser Ser Gly Phe Asp Ala Ser Pro Phe Arg Ser Ser Arg His Met

275 280 285

Ala Tyr Tyr Arg

290

<210> 55

<211> 294

<212> PRT

<213> 橙(Citrus sinensis)

<220>

<221> MISC_FEATURE

<222> (1)..(294)

<223> ABC转运体

<400> 55

Met Ala Asp Gly Met Asn Glu Asn Glu Lys Leu Asn Ser Ile Lys Val

1 5 10 15

Cys Gly Met Gln Phe Ser Tyr Glu Gly Asn Asp Lys Pro Pro Leu Phe

20 25 30

Tyr Asp Phe Asn Leu Gly Ile Ser Pro Gly Ser Arg Cys Leu Leu Val

35 40 45

Gly Ala Asn Gly Ser Gly Lys Thr Thr Leu Leu Lys Ile Leu Ala Gly

50 55 60

Lys His Met Val Gly Gly Arg Asp Val Val Gln Val Leu Asn Arg Ser

65 70 75 80

Ser Phe His Asp Thr Gln Leu Val Cys Ser Gly Asp Leu Ser Tyr Leu

85 90 95

Gly Gly Ser Trp Ser Lys Thr Val Gly Ser Ala Gly Glu Ile Pro Leu

100 105 110

Gln Gly Asp Phe Ser Ala Glu His Met Ile Phe Gly Val Glu Gly Ser

115 120 125

Asp Pro Val Arg Arg Glu Arg Leu Ile Glu Leu Leu Asp Ile Asp Leu

130 135 140

Gln Trp Arg Met His Lys Val Ser Asp Gly Gln Arg Arg Arg Val Gln

145 150 155 160

Ile Cys Met Gly Leu Leu His Pro Phe Lys Val Leu Leu Leu Asp Glu

165 170 175

Ile Thr Val Asp Leu Asp Val Val Ala Arg Met Asp Leu Leu Asp Phe

180 185 190

Phe Lys Asp Glu Cys Glu Gln Arg Gly Ala Thr Ile Val Tyr Ala Thr

195 200 205

His Ile Phe Asp Gly Leu Glu Thr Trp Ala Thr His Leu Ala Tyr Ile

210 215 220

Gln Asp Gly Glu Leu Arg Arg Ala Glu Lys Leu Ala Glu Leu Asp Glu

225 230 235 240

Leu Arg Asn Ser Thr Asn Leu Leu Ser Val Val Glu Ser Trp Leu Arg

245 250 255

Ser Glu Thr Lys Leu Glu Lys Lys Arg Pro Val Asp Pro Pro Lys Gln

260 265 270

Val Gln Lys Thr Ser Pro Phe Gly Ser Ser Pro Phe Met Ser Ser Arg

275 280 285

His Met Ala Tyr Asn Arg

290

<210> 56

<211> 295

<212> PRT

<213> 无油樟(Amborella trichopoda)

<220>

<221> MISC_FEATURE

<222> (1)..(295)

<223> ABC转运体

<400> 56

Met Gly Phe Glu Lys Glu Ala Leu Gln Phe Gln Leu Thr Asn Ser Ile

1 5 10 15

Glu Val Ser Ala Leu Gln Phe Ser Tyr Asp Asp Gln Pro Pro Leu Phe

20 25 30

Ser Asn Phe Asn Leu His Ile Ser Pro Gly Ser Arg Cys Leu Leu Ile

35 40 45

Gly Ala Asn Gly Ser Gly Lys Thr Thr Leu Leu Arg Ile Leu Ala Gly

50 55 60

Lys His Met Val Gly Gly Arg Asp Val Val Arg Val Ile Gly His Ser

65 70 75 80

Ala Phe His Asp Thr Gln Leu Val Cys Ser Gly Glu Leu Ala Tyr Leu

85 90 95

Gly Gly Ser Trp Ser Lys Thr Val Gly Ser Ala Gly Glu Ile Pro Leu

100 105 110

Gln Gly Asp Phe Ser Ala Glu His Met Ile Phe Gly Val Glu Gly Val

115 120 125

Asp Pro Glu Arg Arg Glu Lys Leu Ile Asp Leu Leu Asp Ile Asp Leu

130 135 140

Gln Trp Arg Met His Lys Val Ser Asp Gly Gln Arg Arg Arg Val Gln

145 150 155 160

Ile Cys Met Gly Leu Leu His Pro Phe Gln Val Leu Leu Leu Asp Glu

165 170 175

Val Thr Val Asp Leu Asp Val Val Ala Arg Met Asp Leu Leu Asp Phe

180 185 190

Phe Lys Glu Glu Cys Glu Gly Arg Gly Ala Thr Ile Val Tyr Ala Thr

195 200 205

His Ile Phe Asp Gly Leu Glu Ser Trp Ala Thr His Val Ala Tyr Ile

210 215 220

Gln Asp Gly Glu Leu Arg Arg Ser Glu Lys Leu Ala Asp Val Asn Glu

225 230 235 240

Met Lys Ser Ala Lys Asn Leu Tyr Ser Val Val Glu Ser Trp Leu Arg

245 250 255

Ser Glu Thr Lys Thr Met Thr Lys Lys Ala Phe Asn Pro Ala Ser His

260 265 270

Val Arg Arg Val Pro Thr Val Asn Asp Ala Ser Pro Phe Arg Ser Ser

275 280 285

Arg His Met Ala Tyr Tyr Arg

290 295

<210> 57

<211> 301

<212> PRT

<213> 雷蒙德氏棉(Gossypium raimondii)

<220>

<221> MISC_FEATURE

<222> (1)..(301)

<223> ABC转运体

<400> 57

Met Ala Glu Gln Ala Cys Cys Arg Ser Gly Glu Gly Lys Glu Asn Gly

1 5 10 15

Gly Asp Ser Gln Gly Ile Thr Val Cys Gly Met Gln Phe Ala Tyr Glu

20 25 30

Leu Gln His Pro Leu Phe Phe Asp Phe Asn Leu Asp Ile Ala Arg Gly

35 40 45

Ser Arg Cys Leu Leu Val Gly Ala Asn Gly Ser Ala Gly Lys Thr Thr

50 55 60

Leu Leu Lys Ile Leu Ala Gly Lys His Met Val Gly Gly Arg Glu Val

65 70 75 80

Val Arg Val Leu Asn Arg Ser Ala Phe His Asp Thr Gln Leu Val Cys

85 90 95

Gly Gly Asp Leu Ala Tyr Leu Gly Gly Ser Trp Ser Lys Thr Ile Gly

100 105 110

Ser Ala Gly Glu Val Pro Leu Gln Gly Asp Phe Ser Ala Glu His Met

115 120 125

Ile Phe Gly Val Glu Gly Ile Asp Pro Val Arg Arg Asp Lys Leu Ile

130 135 140

Glu Leu Leu Asp Ile Asp Leu Gln Trp Arg Met His Lys Val Ser Asp

145 150 155 160

Gly Gln Arg Arg Arg Val Gln Ile Cys Met Gly Leu Leu His Pro Phe

165 170 175

Gln Val Leu Leu Leu Asp Glu Val Thr Val Asp Leu Asp Val Val Ala

180 185 190

Arg Met Asp Leu Leu Asp Phe Phe Lys Glu Glu Cys Glu Gln Arg Gly

195 200 205

Ala Thr Val Val Tyr Ala Thr His Ile Phe Asp Gly Leu Glu Thr Trp

210 215 220

Ala Thr His Leu Ala Tyr Ile Gln Asp Gly Gln Leu Lys Arg Ser Glu

225 230 235 240

Lys Leu Thr Glu Ile Asn Glu Leu Lys Ser Ser Glu Asn Leu Leu Ser

245 250 255

Val Val Glu Ala Trp Leu Arg Ser Glu Thr Lys Cys Glu Lys Lys Lys

260 265 270

Pro Ser Asn Ser Pro Ala Gln Val Gln Lys Ser Ser Pro Phe Gly Thr

275 280 285

Ser Pro Phe Met Ser Ser Arg His Met Ala Tyr Tyr Arg

290 295 300

<210> 58

<211> 300

<212> PRT

<213> 胡桃(Juglans regia)

<220>

<221> MISC_FEATURE

<222> (1)..(300)

<223> ABC转运体

<400> 58

Met Ala Asp Lys Glu Thr Ser Lys Pro Ile Val Glu Glu Val Glu Ser

1 5 10 15

Glu Asp Leu Asn Ser Ile Arg Val Cys Gly Met Gln Phe Ala Tyr Glu

20 25 30

Gly Glu Pro Pro Leu Phe Ala Asp Phe Asn Leu Lys Ile Pro Pro Gly

35 40 45

Ser Arg Cys Leu Leu Val Gly Ala Asn Gly Ser Gly Lys Thr Thr Leu

50 55 60

Leu Lys Ile Leu Ala Gly Lys His Met Val Gly Gly Arg Asp Val Val

65 70 75 80

Arg Val Leu Asn Asn Ser Ala Phe His Asp Thr Lys Leu Val Cys Ser

85 90 95

Gly Asp Leu Ala Tyr Leu Gly Gly Ser Trp Ser Lys Thr Val Gly Ser

100 105 110

Ala Gly Glu Ile Pro Leu Gln Gly Asp Phe Ser Ala Glu His Met Ile

115 120 125

Phe Gly Val Glu Gly Val Asp Pro Asp Arg Arg Leu Asn Leu Ile Glu

130 135 140

Leu Leu Asp Ile Asp Leu Gln Trp Arg Met His Lys Val Ser Asp Gly

145 150 155 160

Gln Arg Arg Arg Val Gln Ile Cys Met Gly Leu Leu His Pro Phe Lys

165 170 175

Val Leu Leu Leu Asp Glu Val Thr Val Asp Leu Asp Val Val Ala Arg

180 185 190

Leu Asp Leu Leu Asp Phe Phe Lys Glu Glu Cys Asp Gln Arg Gly Ala

195 200 205

Thr Ile Val Tyr Ala Thr His Ile Phe Asp Gly Leu Glu Thr Trp Ala

210 215 220

Thr His Leu Ala Tyr Ile Gln Asp Gly Glu Leu Arg Arg Val Gln Lys

225 230 235 240

Leu Ser Glu Val Asn Glu Leu Lys Asn Ser Ala Asn Leu Leu Ser Val

245 250 255

Val Glu Ser Trp Leu Arg Ala Glu Thr Lys Arg Glu Lys Lys Lys Pro

260 265 270

Thr Asn Pro Pro Ala Gln Ile Gln Lys Thr Ser Pro Phe Gly Ser Ser

275 280 285

Pro Phe Met Ser Ser Arg His Met Ala Tyr Phe Arg

290 295 300

<210> 59

<211> 274

<212> PRT

<213> 毛果杨(Populus trichocarpa)

<220>

<221> MISC_FEATURE

<222> (1)..(274)

<223> ABC转运体

<400> 59

Met Gln Phe Ala Tyr Pro Gly Gln His Pro Leu Phe Tyr Glu Phe Asn

1 5 10 15

Leu Asn Ile Ser Pro Gly Ser Arg Cys Leu Leu Val Gly Ala Asn Gly

20 25 30

Ser Gly Lys Thr Thr Leu Leu Lys Ile Met Ala Gly Lys His Met Val

35 40 45

Gly Gly Arg Asp Val Val Arg Val Ile Asn Gly Ser Ala Phe His Asp

50 55 60

Thr Gln Leu Val Cys Ser Gly Asp Leu Ala Tyr Leu Gly Gly Ser Trp

65 70 75 80

Ser Lys Thr Val Gly Ser Ala Gly Glu Ile Pro Leu Gln Gly Asp Phe

85 90 95

Ser Ala Glu His Met Ile Phe Gly Val Glu Gly Thr Asp Pro Val Arg

100 105 110

Arg Glu Lys Leu Ile Glu Leu Leu Asp Ile Asp Leu Lys Trp Arg Met

115 120 125

His Lys Val Ser Asp Gly Gln Arg Arg Arg Val Gln Ile Cys Met Gly

130 135 140

Leu Leu His Pro Phe Lys Val Leu Leu Leu Asp Glu Val Thr Val Asp

145 150 155 160

Leu Asp Val Val Ala Arg Met Asp Leu Leu Glu Phe Phe Lys Glu Glu

165 170 175

Cys Asp Gln Arg Gly Ala Thr Ile Val Tyr Ala Thr His Ile Phe Asp

180 185 190

Gly Leu Glu Thr Trp Ala Thr His Leu Ala Tyr Ile Gln Asp Gly Glu

195 200 205

Leu Lys Arg Val Lys Lys Leu Thr Glu Val His Glu Leu Lys Asn Ser

210 215 220

Ala Thr Leu Leu Ser Val Val Glu Ser Trp Leu Arg Ser Glu Thr Lys

225 230 235 240

Asn Glu Lys Lys Lys Pro Thr Asn Pro Pro Ala Gln Asn Gln Lys Thr

245 250 255

Ser Pro Phe Gly Ser Ser Pro Phe Met Ser Ser Arg His Met Ala Tyr

260 265 270

Tyr Arg

<210> 60

<211> 299

<212> PRT

<213> 蒺藜苜蓿(Medicago truncatula)

<220>

<221> MISC_FEATURE

<222> (1)..(299)

<223> ABC转运体

<400> 60

Met Ala Ile Glu Gln Lys Gln Gln Glu Asn Glu Asp Ser Ser Asn Gly

1 5 10 15

Ile Arg Val Gln Gly Met Gln Phe Ser Tyr Asn Asp Ile Gln Gln Gln

20 25 30

Gln Pro Pro Leu Phe Val Asp Phe Asn Leu Gln Val Ser Pro Gly Ser

35 40 45

Arg Cys Leu Leu Val Gly Ala Asn Gly Ser Gly Lys Thr Thr Leu Leu

50 55 60

Lys Ile Leu Ala Gly Lys His Met Val Gly Gly Lys Asp Val Val Arg

65 70 75 80

Val Leu Asn Cys Ser Ala Phe His Asp Thr Gln Leu Val Cys Ser Gly

85 90 95

Asp Leu Ala Tyr Leu Gly Gly Ser Trp Ser Lys Asn Val Gly Cys Ala

100 105 110

Gly Asp Ile Pro Leu Gln Gly Asp Phe Ser Ala Glu His Met Ile Phe

115 120 125

Gly Val Glu Gly Val Asp Pro Glu Arg Arg Asp Lys Leu Ile Glu Leu

130 135 140

Leu Asp Ile Asp Leu Gln Trp Arg Met His Lys Val Ser Asp Gly Gln

145 150 155 160

Arg Arg Arg Val Gln Ile Cys Leu Gly Leu Leu His Pro Tyr Lys Val

165 170 175

Leu Leu Leu Asp Glu Val Thr Val Asp Leu Asp Val Val Thr Arg Met

180 185 190

Asp Leu Leu Glu Phe Phe Lys Glu Glu Cys Glu Gln Arg Glu Ala Thr

195 200 205

Val Val Tyr Ala Thr His Ile Phe Asp Gly Leu Glu Thr Trp Ala Thr

210 215 220

His Leu Ala Tyr Ile Gln Glu Gly Glu Leu Arg Arg Ala Glu Lys Ile

225 230 235 240

Ser Asp Val Asn Glu Leu Lys Ser Ser Ile Asn Leu Leu Ser Val Val

245 250 255

Glu Ser Trp Leu Arg Ala Glu Thr Lys Leu Glu Lys Lys Lys Pro Val

260 265 270

Gln Asn Thr Ser His Ala Gln Gly Asn Ser Phe Thr Asn Ser Pro Phe

275 280 285

Ser Ser Ser Ser Arg His Met Ala Tyr Tyr Arg

290 295

<210> 61

<211> 294

<212> PRT

<213> 菠菜(Spinacia oleracea)

<220>

<221> MISC_FEATURE

<222> (1)..(294)

<223> ABC转运体

<400> 61

Met Val Gln Glu Asn Gly Asn Val Ser Gly Asn Ser Ser Ser Ser Ile

1 5 10 15

Lys Val Gln Ala Met Gln Phe Ala Tyr Pro Gly Gln Pro Pro Ile Phe

20 25 30

Val Asp Phe Ser Leu Gln Ile Ala Pro Gly Ser Arg Cys Leu Leu Val

35 40 45

Gly Ala Asn Gly Ser Gly Lys Thr Thr Leu Leu Lys Ile Leu Ala Gly

50 55 60

Lys His Met Val Gly Gly Arg Asp Val Val Gln Val Leu Asn Ser Ser

65 70 75 80

Ala Phe His Asp Thr His Leu Val Cys Gly Gly Asp Leu Ala Tyr Leu

85 90 95

Gly Gly Ser Trp Ser Lys Thr Ile Gly Cys Ala Gly Asp Val Pro Leu

100 105 110

Gln Gly Asp Ile Ser Ala Glu Gln Met Leu Phe Gly Val Glu Gly Thr

115 120 125

Asp Pro Asp Arg Arg Asp Lys Leu Ile Glu Leu Leu Asp Ile Asp Leu

130 135 140

Gln Trp Arg Met His Lys Val Ser Asp Gly Gln Arg Arg Arg Val Gln

145 150 155 160

Ile Cys Met Gly Leu Leu His Pro Phe Lys Val Leu Leu Leu Asp Glu

165 170 175

Val Thr Val Asp Leu Asp Val Val Ala Arg Met Asp Leu Leu Glu Phe

180 185 190

Leu Lys Glu Glu Cys Asp Gln Arg Gly Ala Thr Ile Val Tyr Ala Thr

195 200 205

His Ile Phe Asp Gly Leu Glu Thr Trp Ala Thr His Leu Ala Tyr Ile

210 215 220

Gln Glu Gly Glu Leu Arg Lys Val Glu Lys Met Ser Glu Leu His Glu

225 230 235 240

Leu Lys Asn Ser Thr Asn Leu Leu Ser Val Val Glu Ala Trp Leu Arg

245 250 255

Ser Glu Thr Lys Leu Glu Lys Lys Lys Pro Val Ala Thr Ile Thr Asn

260 265 270

Asn Gln Lys Ser Ser Gly Phe Asp Ala Ser Pro Phe Arg Ser Ser Arg

275 280 285

His Met Ala Tyr Tyr Arg

290

<210> 62

<211> 296

<212> PRT

<213> 大豆(Glycine max)

<220>

<221> MISC_FEATURE

<222> (1)..(296)

<223> ABC转运体

<400> 62

Met Asn Ser Ser Leu Ser Gly Asn Gly Gly Ser Thr Thr Met Ala Ala

1 5 10 15

Glu Asn Glu Asp Ser Cys Gly Ile Arg Val Thr Gly Met Gln Phe Ser

20 25 30

Tyr Asp Val Gln Gln Pro Pro Leu Phe Leu Asp Phe Asn Leu Asn Val

35 40 45

Ser Pro Gly Ser Arg Cys Leu Leu Val Gly Ala Asn Gly Ser Gly Lys

50 55 60

Thr Thr Leu Leu Lys Ile Leu Ala Gly Lys His Met Val Gly Gly Arg

65 70 75 80

Asp Val Val Arg Val Leu Ser Gly Ser Ala Phe His Asp Thr Gln Leu

85 90 95

Val Cys Ser Gly Asp Leu Ala Tyr Leu Gly Gly Ser Trp Ser Lys Asn

100 105 110

Val Gly Ser Ala Gly Glu Ile Pro Leu Gln Gly Asp Phe Ser Ala Glu

115 120 125

His Met Ile Phe Gly Val Glu Gly Ala Asp Pro Glu Arg Arg Asp Lys

130 135 140

Leu Ile Glu Leu Leu Asp Ile Asp Leu Gln Trp Arg Met His Lys Val

145 150 155 160

Ser Asp Gly Gln Arg Arg Arg Val Gln Ile Cys Leu Gly Leu Leu His

165 170 175

Pro Tyr Lys Val Leu Leu Leu Asp Glu Val Thr Val Asp Leu Asp Val

180 185 190

Val Thr Arg Met Asp Leu Leu Asp Phe Phe Lys Glu Glu Cys Glu Gln

195 200 205

Arg Glu Ala Ile Ile Val Tyr Ala Thr His Ile Phe Asp Gly Leu Glu

210 215 220

Thr Trp Ala Thr His Leu Ala Tyr Ile Gln Asp Gly Glu Leu Arg Arg

225 230 235 240

Ala Glu Lys Ile Ser Asn Val Lys Glu Leu Lys Ser Ser Thr Asn Leu

245 250 255

Leu Ser Val Val Glu Ala Trp Leu Arg Ala Glu Thr Lys Leu Glu Lys

260 265 270

Lys Asn Pro Val Gln Lys Thr Ser Val Ala Ser Ser Pro Phe Phe Ser

275 280 285

Ser Arg His Met Ala Tyr Tyr Arg

290 295

<210> 63

<211> 287

<212> PRT

<213> 苹果(Malus domestica)

<220>

<221> MISC_FEATURE

<222> (1)..(287)

<223> ABC转运体

<400> 63

Met Ala Glu Glu Ala Ser Ser Gly Ile Arg Val Gln Gly Met Gln Phe

1 5 10 15

Ala Tyr Asp Ala Gln Pro Pro Leu Phe Cys Asp Phe Ser Leu Glu Ile

20 25 30

Thr Pro Gly Ser Arg Cys Leu Leu Ile Gly Ala Asn Gly Ser Gly Lys

35 40 45

Thr Thr Leu Leu Lys Ile Leu Ala Gly Lys Gln Met Val Gly Gly Arg

50 55 60

Asp Val Val Arg Val Leu Asn Cys Ser Ala Phe His Asp Thr His Leu

65 70 75 80

Val Cys Ser Gly Asp Leu Ala Tyr Leu Gly Gly Ser Trp Thr Lys Thr

85 90 95

Val Gly Ser Ala Gly Glu Leu Pro Leu Gln Gly Asp Phe Ser Ala Glu

100 105 110

His Met Ile Phe Gly Val Glu Gly Thr Asp Pro Val Arg Arg Asp Lys

115 120 125

Leu Ile Glu Leu Leu Asp Ile Asp Leu Arg Trp Arg Met His Lys Val

130 135 140

Ser Asp Gly Gln Arg Arg Arg Val Gln Ile Cys Met Gly Leu Leu His

145 150 155 160

Pro Phe Lys Val Leu Leu Leu Asp Glu Val Thr Val Asp Leu Asp Val

165 170 175

Val Ala Arg Met Asp Leu Leu Asp Phe Phe Lys Glu Glu Cys Glu Gln

180 185 190

Arg Gly Ala Thr Ile Val Tyr Ala Thr His Ile Phe Asp Gly Leu Glu

195 200 205

Thr Trp Ala Thr His Leu Ala Tyr Ile Gln Asp Gly Glu Leu Lys Arg

210 215 220

Ala Glu Lys Leu Ser Glu Val Asn Glu Leu Lys Xaa Ser Val Asn Leu

225 230 235 240

Leu Ser Val Val Glu Ser Trp Leu Arg Ala Glu Thr Lys Ser Asp Lys

245 250 255

Lys Lys Ser Ser Asn Pro Pro Ala Gln Ile Lys Lys Thr Ser Pro Phe

260 265 270

Ala Ser Ser Ala Phe Thr Ser Ser Arg His Met Ala Tyr Tyr Arg

275 280 285

<210> 64

<211> 296

<212> PRT

<213> 野大豆(Glycine soja)

<220>

<221> MISC_FEATURE

<222> (1)..(296)

<223> ABC转运体

<400> 64

Met Asn Ser Ser Leu Ser Gly Asn Gly Gly Ser Thr Thr Met Ala Ala

1 5 10 15

Glu Asn Glu Asp Ser Cys Gly Ile Arg Val Thr Gly Met Gln Phe Ser

20 25 30

Tyr Asp Val Gln Gln Pro Pro Leu Phe Leu Asp Phe Asn Leu Asn Val

35 40 45

Ser Pro Gly Ser Arg Cys Leu Leu Val Gly Ala Asn Gly Ser Gly Lys

50 55 60

Thr Thr Leu Leu Lys Ile Leu Ala Gly Lys His Met Val Gly Gly Arg

65 70 75 80

Asp Val Val Arg Val Leu Ser Gly Ser Ala Phe His Asp Thr Gln Leu

85 90 95

Val Cys Ser Gly Asp Leu Ala Tyr Leu Gly Gly Ser Trp Ser Lys Asn

100 105 110

Val Gly Ser Ala Gly Glu Ile Pro Leu Gln Gly Asp Phe Ser Ala Glu

115 120 125

His Met Ile Phe Gly Val Glu Gly Ala Asp Pro Glu Arg Arg Asp Lys

130 135 140

Leu Ile Glu Leu Leu Asp Ile Asp Leu Gln Trp Arg Met His Lys Val

145 150 155 160

Ser Asp Gly Gln Arg Arg Arg Val Gln Ile Cys Leu Gly Leu Leu His

165 170 175

Pro Tyr Lys Val Leu Leu Leu Asp Glu Val Thr Val Asp Leu Asp Val

180 185 190

Val Thr Arg Met Asp Leu Leu Asp Phe Phe Lys Glu Glu Cys Glu Gln

195 200 205

Arg Glu Ala Ile Ile Val Tyr Ala Thr His Ile Phe Asp Gly Leu Glu

210 215 220

Thr Trp Ala Thr His Leu Ala Tyr Ile Gln Asp Gly Glu Leu Arg Arg

225 230 235 240

Ala Glu Lys Ile Ser Asn Val Asn Glu Leu Lys Ser Ser Thr Asn Leu

245 250 255

Leu Ser Val Val Glu Ala Trp Leu Arg Ala Glu Thr Lys Leu Glu Lys

260 265 270

Lys Asn Pro Val Gln Lys Thr Ser Val Ala Ser Ser Pro Phe Phe Ser

275 280 285

Ser Arg His Met Ala Tyr Tyr Arg

290 295

<210> 65

<211> 286

<212> PRT

<213> 中粒咖啡(Coffea canephora)

<220>

<221> MISC_FEATURE

<222> (1)..(286)

<223> ABC转运体

<400> 65

Met Glu Glu Ser Ser Cys Ser Ile Gln Val Asn Gly Met Gln Phe Ala

1 5 10 15

Tyr Glu Ile Gln Thr Pro Leu Phe Phe Asp Phe Thr Ile Asn Ile Ser

20 25 30

Pro Gly Ser Arg Cys Leu Leu Val Gly Ser Asn Gly Ser Gly Lys Thr

35 40 45

Thr Leu Leu Lys Ile Leu Ala Gly Lys His Met Val Gly Gly Arg Asp

50 55 60

Val Val Arg Val Leu Asn Phe Ser Ala Phe His Asp Thr Asn Leu Val

65 70 75 80

Cys Gly Gly Asp Leu Ala Tyr Leu Gly Asp Ser Trp Ser Lys Asn Val

85 90 95

Gly Ser Ala Gly Glu Ile Pro Leu Gln Gly Asp Phe Ser Ala Glu His

100 105 110

Met Ile Phe Gly Val Glu Gly Val Asp Pro Val Arg Arg Glu Lys Leu

115 120 125

Ile Glu Leu Leu Asp Ile Asp Leu His Trp Arg Met His Lys Val Ser

130 135 140

Asp Gly Gln Arg Arg Arg Val Gln Ile Cys Met Gly Leu Leu His Pro

145 150 155 160

Phe Lys Val Leu Leu Leu Asp Glu Val Thr Val Asp Leu Asp Val Val

165 170 175

Ala Arg Met Asp Leu Leu Asp Phe Phe Arg Glu Glu Cys Glu Gln Arg

180 185 190

Gly Ala Thr Ile Val Tyr Ala Thr His Ile Phe Asp Gly Leu Glu Thr

195 200 205

Trp Ala Thr Asp Ile Val Tyr Ile Gln Glu Gly Ile Leu Lys Arg His

210 215 220

Glu Lys Leu Ser Asp Leu Ser Glu Leu Lys Ala Ser Pro Asn Leu Leu

225 230 235 240

Ser Val Val Glu Ser Trp Leu Arg Ser Glu Thr Lys Val Glu Lys Lys

245 250 255

Lys His Ile Thr Ala Gln Ser Pro Val Arg Lys Ser Ser Pro Phe Asp

260 265 270

Ala Ser Pro Phe Arg Ser Ser Arg His Met Ala Tyr Tyr Arg

275 280 285

<210> 66

<211> 297

<212> PRT

<213> 麻风树(Jatropha curcas)

<220>

<221> MISC_FEATURE

<222> (1)..(297)

<223> ABC转运体

<400> 66

Met Ala Glu Lys Ala Trp Lys Asn Glu Glu Ser Glu Asn Glu Lys Gln

1 5 10 15

Asn Ser Ile Ser Val Val Gly Met Gln Phe Ala Tyr Glu Gly Gln Ser

20 25 30

Pro Leu Phe Tyr Gly Phe Asn Leu Asn Thr Leu Pro Gly Ser Arg Tyr

35 40 45

Leu Leu Val Gly Ala Asn Gly Ser Gly Lys Thr Thr Leu Leu Arg Ile

50 55 60

Leu Ala Gly Lys His Met Val Gly Gly Arg Asp Val Val Gln Val Leu

65 70 75 80

Asn Arg Ser Ala Phe His Asp Thr Gln Leu Val Cys Ser Gly Glu Leu

85 90 95

Ala Tyr Leu Gly Gly Ser Trp Ser Lys Thr Val Gly Ser Ala Gly Glu

100 105 110

Ile Pro Leu Gln Gly Asp Phe Ser Ala Glu Gln Met Ile Phe Gly Val

115 120 125

Glu Gly Ile Asp Pro Ala Arg Arg Glu Lys Leu Ile Asp Leu Leu Asp

130 135 140

Ile Asp Leu Gln Trp Arg Met His Lys Val Ser Asp Gly Gln Arg Arg

145 150 155 160

Arg Val Gln Ile Cys Met Gly Leu Leu Val Pro Phe Lys Val Leu Leu

165 170 175

Leu Asp Glu Val Thr Val Asp Leu Asp Val Val Ala Arg Ile Asp Leu

180 185 190

Leu Glu Phe Phe Lys Gln Glu Cys Glu Gln Arg Gly Ala Thr Ile Val

195 200 205

Tyr Ala Thr His Ile Phe Asp Gly Leu Glu Thr Trp Ala Thr His Leu

210 215 220

Ala Tyr Ile Gln Asp Gly Glu Leu Arg Arg Ala Lys Lys Leu Thr Glu

225 230 235 240

Val Ser Glu Leu Arg Ser Ser Ala Asn Leu Leu Ser Val Val Glu Ser

245 250 255

Trp Leu Arg Ser Glu Thr Lys Ser Glu Lys Glu Lys Pro Thr Asn Pro

260 265 270

Pro Pro Glu Lys Gln Lys Thr Ser Pro Phe Gly Ala Ser Pro Phe Met

275 280 285

Ser Ser Arg His Met Ala Tyr Tyr Arg

290 295

<210> 67

<211> 290

<212> PRT

<213> 蔓花生(Arachis duranensis)

<220>

<221> MISC_FEATURE

<222> (1)..(290)

<223> ABC转运体

<400> 67

Met Ala Val Glu Glu Glu Lys Glu Gly Ser Met Gly Ile Arg Val His

1 5 10 15

Gly Met Gln Phe Ser Tyr Glu Trp Gln Ser Pro Leu Phe Val Asp Phe

20 25 30

Lys Leu Asn Val Ser Pro Gly Ser Arg Cys Leu Leu Leu Gly Ala Asn

35 40 45

Gly Ser Gly Lys Thr Thr Leu Leu Lys Ile Leu Ala Gly Lys His Met

50 55 60

Val Gly Gly Arg Asp Val Val Arg Val Leu Asn Gly Ser Ala Phe His

65 70 75 80

Asp Thr His Leu Val Cys Ser Gly Asp Leu Ala Tyr Leu Gly Gly Ser

85 90 95

Trp Ser Lys Thr Ile Gly Ser Ala Gly Asp Val Pro Leu Gln Gly Asp

100 105 110

Phe Ser Ala Glu His Met Ile Phe Gly Val Glu Gly Ala Asp Pro Asp

115 120 125

Arg Arg Asn Lys Leu Ile Glu Leu Leu Asp Ile Asp Leu Gln Trp Arg

130 135 140

Met His Lys Val Ser Asp Gly Gln Arg Arg Arg Val Gln Ile Cys Leu

145 150 155 160

Gly Leu Leu His Pro Phe Lys Val Leu Leu Leu Asp Glu Val Thr Val

165 170 175

Asp Leu Asp Val Val Thr Arg Met Asp Leu Leu Glu Phe Phe Lys Glu

180 185 190

Glu Cys Glu Gln Arg Gln Ala Thr Ile Val Tyr Ala Thr His Ile Phe

195 200 205

Asp Gly Leu Glu Thr Trp Ala Thr His Leu Ala Tyr Ile Gln Ala Gly

210 215 220

Glu Leu Arg Arg Ala Glu Lys Leu Ser Asp Val Asp Glu Leu Lys Ser

225 230 235 240

Ser Ala Asn Leu Leu Ser Val Val Glu Thr Trp Leu Arg Ala Glu Thr

245 250 255

Arg Asn Glu Lys Lys Lys Pro Val Gln Asn Thr Ala Gln Thr Gln Lys

260 265 270

Thr Ser Val Ala Ser Ser Pro Phe Phe Ser Ser Arg His Met Ala Tyr

275 280 285

Tyr Arg

290

<210> 68

<211> 290

<212> PRT

<213> 塔里哈花生(Arachis ipaensis)

<220>

<221> MISC_FEATURE

<222> (1)..(290)

<223> ABC转运体

<400> 68

Met Ala Val Glu Glu Glu Lys Glu Gly Ser Met Gly Ile Arg Val His

1 5 10 15

Gly Met Gln Phe Ser Tyr Glu Trp Gln Ser Pro Leu Phe Val Asp Phe

20 25 30

Lys Leu Asn Val Ser Pro Gly Ser Arg Cys Leu Leu Leu Gly Ala Asn

35 40 45

Gly Ser Gly Lys Thr Thr Leu Leu Lys Ile Leu Ala Gly Lys His Met

50 55 60

Val Gly Gly Arg Asp Val Val Arg Val Leu Asn Gly Ser Ala Phe His

65 70 75 80

Asp Thr His Leu Val Cys Ser Gly Asp Leu Ala Tyr Leu Gly Gly Ser

85 90 95

Trp Ser Lys Thr Ile Gly Ser Ala Gly Asp Val Pro Leu Gln Gly Asp

100 105 110

Phe Ser Ala Glu His Met Ile Phe Gly Val Glu Gly Ala Asp Pro Asp

115 120 125

Arg Arg Asn Lys Leu Ile Glu Leu Leu Asp Ile Asp Leu Gln Trp Arg

130 135 140

Met His Lys Val Ser Asp Gly Gln Arg Arg Arg Val Gln Ile Cys Leu

145 150 155 160

Gly Leu Leu His Pro Phe Lys Val Leu Leu Leu Asp Glu Val Thr Val

165 170 175

Asp Leu Asp Val Val Thr Arg Met Asp Leu Leu Glu Phe Phe Lys Glu

180 185 190

Glu Cys Glu Gln Arg Gln Ala Thr Ile Val Tyr Ala Thr His Ile Phe

195 200 205

Asp Gly Leu Glu Thr Trp Ala Thr His Leu Ala Tyr Ile Gln Ala Gly

210 215 220

Glu Leu Arg Lys Ala Glu Lys Leu Ser Asp Val Asp Glu Leu Lys Ser

225 230 235 240

Ser Ala Asn Leu Leu Ser Val Val Glu Thr Trp Leu Arg Ala Glu Thr

245 250 255

Arg Asn Glu Lys Lys Lys Pro Val Gln Asn Thr Ala Gln Thr Gln Lys

260 265 270

Thr Ser Val Ala Ser Ser Pro Phe Phe Ser Ser Arg His Met Ala Tyr

275 280 285

Tyr Arg

290

<210> 69

<211> 290

<212> PRT

<213> 甘蓝型油菜(Brassica napus)

<220>

<221> MISC_FEATURE

<222> (1)..(290)

<223> ABC转运体

<400> 69

Met Ala Ala Lys Asp Gln Ala Thr Thr Ser Asp Asp Ala Ile Arg Val

1 5 10 15

Ser Gly Met Glu Phe Ser Tyr Glu Ala Glu Asp Pro Ile Phe Phe Asp

20 25 30

Phe Asn Leu Asp Leu Pro Ala Gly Ser Arg Cys Leu Leu Val Gly Ala

35 40 45

Asn Gly Ser Gly Lys Thr Thr Leu Leu Arg Ile Leu Ala Gly Lys His

50 55 60

Met Val Gly Gly Lys Asn Val Val Gln Val Leu Ser Arg Ser Ala Phe

65 70 75 80

His Asp Thr Gln Leu Val Cys Ser Gly Asp Leu Ser Tyr Leu Gly Gly

85 90 95

Ser Trp Ser Lys Thr Val Gly Ser Ala Gly Glu Val Pro Leu Gln Gly

100 105 110

Asp Phe Ser Ala Glu His Met Ile Phe Gly Val Glu Gly Ile Asp Pro

115 120 125

Val Arg Arg Glu Lys Leu Ile Asp Leu Leu Asp Ile Asn Leu Gln Trp

130 135 140

Arg Met His Lys Val Ser Asp Gly Gln Lys Arg Arg Val Gln Ile Cys

145 150 155 160

Met Gly Leu Leu His Pro Phe Lys Val Leu Leu Leu Asp Glu Val Thr

165 170 175

Val Asp Leu Asp Val Val Ala Arg Met Asp Leu Leu Glu Phe Phe Lys

180 185 190

Glu Glu Cys Asp Gln Arg Gly Ala Thr Ile Val Tyr Ala Thr His Ile

195 200 205

Phe Asp Gly Leu Glu Thr Trp Ala Thr His Leu Ala Tyr Ile Gln Asn

210 215 220

Gly Glu Leu Asn Arg Ser Ser Lys Met Ala Asp Ile Ser Glu Met Lys

225 230 235 240

Thr Ser Pro Asn Leu Leu Ser Val Val Glu Ser Trp Leu Arg Ser Glu

245 250 255

Thr Lys Ile Asp Lys Lys Lys Lys Glu Pro Val Pro Ala Trp Lys Pro

260 265 270

Thr Pro Phe Asp Asn Ser Pro Phe Arg Ser Ser Arg His Met Ala Tyr

275 280 285

Tyr Arg

290

<210> 70

<211> 286

<212> PRT

<213> 林烟草(Nicotiana sylvestris)

<220>

<221> MISC_FEATURE

<222> (1)..(286)

<223> ABC转运体

<400> 70

Met Glu Gly Lys Ser Ser Ser Ile Gln Val Asn Gly Met Gln Phe Ser

1 5 10 15

Tyr Asp Phe Gln Ser Pro Ile Phe Phe Asp Phe Ser Leu Lys Ile Ser

20 25 30

Pro Gly Ser Arg Cys Leu Leu Val Gly Ala Asn Gly Ser Gly Lys Thr

35 40 45

Thr Leu Leu Arg Ile Leu Ala Gly Lys His Met Val Gly Gly Arg Asp

50 55 60

Val Val Lys Val Leu Asn Phe Ser Ala Phe His Asp Thr His Leu Val

65 70 75 80

Cys Asp Gly Asp Leu Ala Tyr Leu Gly Glu Ser Trp Ser Lys Asn Val

85 90 95

Gly Ser Ala Gly Glu Ile Pro Leu Gln Gly Asp Phe Ser Ala Glu His

100 105 110

Met Ile Phe Gly Val Glu Gly Val Asp Pro Val Arg Arg Glu Lys Leu

115 120 125

Ile Glu Leu Leu Asp Ile Asp Leu Gln Trp Arg Met His Lys Val Ser

130 135 140

Asp Gly Gln Arg Arg Arg Val Gln Ile Cys Met Gly Leu Leu His Pro

145 150 155 160

Tyr Lys Val Leu Leu Leu Asp Glu Val Thr Val Asp Leu Asp Val Val

165 170 175

Ala Arg Met Asp Leu Leu Asp Phe Phe Lys Glu Glu Cys Glu Gln Arg

180 185 190

Gly Ala Thr Ile Val Tyr Ala Thr His Ile Phe Asp Gly Leu Glu Ser

195 200 205

Trp Ala Thr Asp Leu Val Tyr Ile Gln Asp Gly Val Leu Lys Arg Ser

210 215 220

Glu Lys Leu Pro Glu Leu Pro Glu Leu Lys Ser Ser Pro Asn Leu Leu

225 230 235 240

Ser Val Val Glu Asn Trp Leu Arg Ser Glu Thr Thr Ile Glu Lys Lys

245 250 255

Lys Pro Val Ile Ala Pro Pro Lys Val Gln Lys Ser Ser Pro Phe Gly

260 265 270

Ser Ser Pro Phe Gln Ser Ser Arg His Met Ala Tyr Phe Arg

275 280 285

<210> 71

<211> 283

<212> PRT

<213> 大豆(Glycine max)

<220>

<221> MISC_FEATURE

<222> (1)..(283)

<223> ABC转运体

<400> 71

Met Ala Ala Glu Asn Glu Asp Ser Cys Gly Ile Arg Val Thr Gly Met

1 5 10 15

Gln Phe Ser Tyr Asp Val Gln Gln Pro Pro Leu Phe Leu Asp Phe Asn

20 25 30

Leu Asn Val Ser Pro Gly Ser Arg Cys Leu Leu Val Gly Ala Asn Gly

35 40 45

Ser Gly Lys Thr Thr Leu Leu Lys Ile Leu Ala Gly Lys His Met Val

50 55 60

Gly Gly Arg Asp Val Val Arg Val Leu Ser Gly Ser Ala Phe His Asp

65 70 75 80

Thr Gln Leu Val Cys Ser Gly Asp Leu Ala Tyr Leu Gly Gly Ser Trp

85 90 95

Ser Lys Asn Val Gly Ser Ala Gly Glu Ile Pro Leu Gln Gly Asp Ser

100 105 110

Ser Ala Glu His Met Ile Phe Gly Val Glu Gly Ala Asp Pro Glu Arg

115 120 125

Arg Asp Lys Leu Ile Glu Leu Leu Asp Ile Asp Leu Gln Trp Arg Met

130 135 140

His Lys Val Ser Asp Gly Gln Arg Arg Arg Val Gln Ile Cys Leu Gly

145 150 155 160

Leu Leu His Pro Tyr Lys Val Leu Leu Leu Asp Glu Val Thr Val Asp

165 170 175

Leu Asp Val Val Thr Arg Met Asp Leu Leu Asp Phe Phe Lys Glu Glu

180 185 190

Cys Glu Gln Arg Glu Ala Ile Ile Val Tyr Ala Thr His Ile Phe Asp

195 200 205

Gly Leu Glu Thr Trp Ala Thr His Leu Ala Tyr Ile Gln Asp Gly Glu

210 215 220

Leu Arg Arg Ala Glu Lys Ile Ser Asn Val Lys Glu Leu Lys Ser Ser

225 230 235 240

Thr Asn Leu Leu Ser Val Val Glu Ala Trp Leu Arg Ala Glu Thr Lys

245 250 255

Leu Glu Lys Lys Asn Pro Val Gln Lys Thr Ser Val Ala Ser Ser Pro

260 265 270

Phe Phe Ser Ser Arg His Met Ala Tyr Tyr Arg

275 280

<210> 72

<211> 287

<212> PRT

<213> 百脉根(Lotus japonicas)

<220>

<221> MISC_FEATURE

<222> (1)..(287)

<223> ABC转运体

<400> 72

Met Ala Ile Gln Asp Ser Glu Arg Ser Gly Gly Gly Gly Ile Arg Val

1 5 10 15

Gln Gly Met Gln Phe Thr Tyr Pro Gly Gln Gln Pro Pro Leu Phe Leu

20 25 30

Asp Phe His Leu Asp Val Ser Pro Gly Ser Arg Cys Leu Leu Leu Gly

35 40 45

Ala Asn Gly Ser Gly Lys Thr Thr Leu Leu Lys Ile Leu Thr Gly Lys

50 55 60

His Met Val Gly Gly Arg Asp Val Val Arg Val Leu Asn Gly Ser Ala

65 70 75 80

Phe His Asp Thr Gln Leu Val Cys Ser Gly Asp Leu Ser Tyr Leu Gly

85 90 95

Gly Ser Trp Ser Lys Thr Ile Gly Cys Ala Gly Glu Ile Pro Leu Gln

100 105 110

Gly Asp Phe Ser Ala Glu His Met Ile Phe Gly Val Glu Gly Ala Asp

115 120 125

Pro Asp Arg Arg Asp Arg Leu Ile Glu Leu Leu Asp Ile Asp Leu Gln

130 135 140

Trp Arg Met His Lys Val Ser Asp Gly Gln Arg Arg Arg Val Gln Ile

145 150 155 160

Cys Leu Gly Leu Leu His Pro Tyr Lys Ala Leu Leu Leu Asp Glu Val

165 170 175

Thr Val Asp Leu Asp Val Val Thr Arg Met Asp Leu Leu Asp Phe Phe

180 185 190

Lys Glu Glu Cys Glu Gln Arg Glu Ala Thr Ile Val Tyr Ala Thr His

195 200 205

Ile Phe Asp Gly Leu Glu Thr Trp Ala Thr His Leu Ala Tyr Ile Gln

210 215 220

Glu Gly Glu Leu Lys Arg Thr Glu Lys Ile Ser Asp Val Asn Glu Leu

225 230 235 240

Lys Ser Ser Ala Asn Leu Leu Ser Val Val Glu Ser Trp Leu Arg Ala

245 250 255

Glu Thr Lys Leu Glu Lys Lys Asn Pro Thr Ser Asn Val Gln Lys Thr

260 265 270

Ser Ser Ser Pro Phe Phe Ser Ser Arg His Met Ala Tyr Tyr Arg

275 280 285

<210> 73

<211> 296

<212> PRT

<213> 菜豆(Phaseolus vulgaris)

<220>

<221> MISC_FEATURE

<222> (1)..(296)

<223> ABC转运体

<400> 73

Met Asn Thr Ser Leu Ser Ser Met Ala Glu Tyr Ser Leu Thr Met Ala

1 5 10 15

Ala Ala Asp Glu Asp Ser Leu Gly Ile Arg Val Arg Gly Met Gln Phe

20 25 30

Ser Tyr Glu Ala Gln Gln Pro Pro Leu Phe Leu Asp Phe Lys Leu Asn

35 40 45

Val Glu Pro Gly Ser Arg Cys Leu Leu Val Gly Ala Asn Gly Ser Gly

50 55 60

Lys Thr Thr Leu Leu Lys Ile Leu Ala Gly Lys His Met Val Gly Gly

65 70 75 80

Arg Asp Val Val Arg Val Leu Asn Gly Ser Ala Phe His Asp Thr Gln

85 90 95

Leu Val Cys Ser Gly Asp Leu Ala Tyr Leu Gly Gly Ser Trp Ser Lys

100 105 110

Asn Val Gly Ser Ala Gly Glu Ile Pro Leu Gln Gly Asp Phe Ser Ala

115 120 125

Glu His Met Ile Phe Gly Val Glu Gly Ala Asp Pro Asp Arg Arg Asp

130 135 140

Lys Leu Ile Glu Leu Leu Asp Ile Asp Leu Gln Trp Arg Met His Lys

145 150 155 160

Val Ser Asp Gly Gln Arg Arg Arg Val Gln Ile Cys Leu Gly Leu Leu

165 170 175

Tyr Pro Tyr Lys Val Leu Leu Leu Asp Glu Val Thr Val Asp Leu Asp

180 185 190

Val Val Thr Arg Met Asp Leu Leu Asp Phe Phe Arg Glu Glu Cys Glu

195 200 205

Gln Arg Glu Ala Thr Ile Val Tyr Ala Thr His Ile Phe Asp Gly Leu

210 215 220

Glu Thr Trp Ala Thr His Leu Ala Tyr Ile Gln Asp Gly Glu Leu Arg

225 230 235 240

Arg Ala Glu Lys Ile Ser Asp Val Asn Glu Leu Lys Ser Ser Ile Asn

245 250 255

Leu Leu Ser Val Val Glu Ala Trp Leu Arg Ala Glu Thr Lys Val Val

260 265 270

Lys Lys Asn Pro Val Gln Lys Thr Phe Ala Ser Asn Pro Phe Phe Ser

275 280 285

Ser Arg His Met Ala Tyr Tyr Arg

290 295

<210> 74

<211> 286

<212> PRT

<213> 绒毛状烟草(Nicotiana tomentosiformis)

<220>

<221> MISC_FEATURE

<222> (1)..(286)

<223> ABC转运体

<400> 74

Met Glu Gly Glu Ser Asn Ser Ile Gln Val Asp Gly Met Gln Phe Ser

1 5 10 15

Tyr Asp Phe Gln Ser Pro Ile Phe Phe Asp Phe Ser Leu Lys Ile Ser

20 25 30

Pro Gly Ser Arg Cys Leu Leu Val Gly Ala Asn Gly Ser Gly Lys Thr

35 40 45

Thr Leu Leu Arg Ile Leu Ala Gly Lys His Met Val Gly Gly Arg Asp

50 55 60

Val Val Lys Val Leu Asn Phe Ser Ala Phe His Asp Thr His Leu Val

65 70 75 80

Cys Asp Gly Asp Leu Ala Tyr Leu Gly Glu Ser Trp Ser Lys Asn Val

85 90 95

Gly Ser Ala Gly Glu Ile Pro Leu Gln Gly Asp Phe Ser Ala Glu His

100 105 110

Met Ile Phe Gly Val Glu Gly Val Asp Pro Val Arg Arg Glu Lys Leu

115 120 125

Ile Glu Leu Leu Asp Ile Asp Leu Gln Trp Arg Met His Lys Val Ser

130 135 140

Asp Gly Gln Arg Arg Arg Val Gln Ile Cys Met Gly Leu Leu His Pro

145 150 155 160

Tyr Lys Val Leu Leu Leu Asp Glu Val Thr Val Asp Leu Asp Val Val

165 170 175

Ala Arg Met Asp Leu Leu Asp Phe Phe Lys Glu Glu Cys Glu Gln Arg

180 185 190

Gly Ala Thr Ile Val Tyr Ala Thr His Ile Phe Asp Gly Leu Glu Ser

195 200 205

Trp Ala Thr Asp Leu Val Tyr Ile Gln Asp Gly Val Leu Lys Arg Ser

210 215 220

Glu Lys Leu Pro Glu Leu Pro Glu Leu Lys Ser Ser Pro Asn Leu Leu

225 230 235 240

Ser Val Val Glu Asn Trp Leu Arg Ser Glu Thr Thr Ile Glu Lys Lys

245 250 255

Lys Pro Val Ile Ala Pro Pro Lys Val Gln Lys Ser Ser Pro Phe Gly

260 265 270

Ser Ser Pro Phe Gln Ser Ser Arg His Met Ala Tyr Phe Arg

275 280 285

<210> 75

<211> 291

<212> PRT

<213> 小花南芥(Arabis alpine)

<220>

<221> MISC_FEATURE

<222> (1)..(291)

<223> ABC转运体

<400> 75

Met Ala Glu Lys His Ala Arg Thr Ser Gly Asp Asp Asp Ala Ile Arg

1 5 10 15

Val Ser Gly Met Glu Phe Gly Tyr Glu Val Gln Asp Pro Ile Phe Phe

20 25 30

Asp Phe Asn Leu Asp Leu Pro Ala Gly Ser Arg Cys Leu Leu Val Gly

35 40 45

Ala Asn Gly Ser Gly Lys Thr Thr Leu Leu Lys Ile Met Ala Gly Lys

50 55 60

His Met Val Gly Gly Lys Asn Val Val Gln Val Leu Ser Arg Ser Ala

65 70 75 80

Phe His Asp Thr Gln Leu Val Cys Ser Gly Asp Leu Ser Tyr Leu Gly

85 90 95

Gly Ser Trp Ser Lys Thr Val Gly Ser Ala Gly Glu Val Pro Leu Gln

100 105 110

Gly Asp Phe Ser Ala Glu His Met Ile Phe Gly Val Glu Gly Thr Asp

115 120 125

Pro Val Arg Arg Glu Lys Leu Ile Asp Leu Leu Asp Ile Asn Leu Asn

130 135 140

Trp Arg Met His Lys Val Ser Asp Gly Gln Lys Arg Arg Val Gln Ile

145 150 155 160

Cys Met Gly Leu Leu His Pro Phe Lys Val Leu Leu Leu Asp Glu Val

165 170 175

Thr Val Asp Leu Asp Val Val Ala Arg Met Asp Leu Leu Glu Phe Phe

180 185 190

Lys Glu Glu Cys Asp Gln Arg Gly Ala Thr Ile Val Tyr Ala Thr His

195 200 205

Ile Phe Asp Gly Leu Glu Thr Trp Ala Thr His Leu Ala Tyr Ile Gln

210 215 220

Asp Gly Gln Leu Asn Arg Ser Ser Ser Met Ala Asp Ile Asn Glu Leu

225 230 235 240

Lys Thr Ala Pro Asn Leu Leu Ser Val Val Glu Ser Trp Leu Arg Ser

245 250 255

Glu Thr Lys Leu Glu Lys Lys Lys Arg Lys Pro Val Ala Ala Trp Lys

260 265 270

Pro Ser Gln Phe Asp Thr Ser Pro Phe Arg Ser Ser Arg His Met Ala

275 280 285

Tyr Tyr Arg

290

<210> 76

<211> 302

<212> PRT

<213> 土瓶草(Cephalotus follicularis)

<220>

<221> MISC_FEATURE

<222> (1)..(302)

<223> ABC转运体

<400> 76

Met Leu Gln Arg Arg Lys Lys Met Gly Glu Glu Glu Glu Asn Glu Lys

1 5 10 15

Pro Trp Ser Asn Ser Ser Ser Ser Ile Arg Val Cys Gly Met Gln Phe

20 25 30

Ala Tyr Asp Ala Gln Ser Pro Val Phe Val Asp Phe Asn Leu Asn Val

35 40 45

Ser Pro Gly Ser Arg Trp Leu Leu Val Gly Ala Asn Gly Ser Gly Lys

50 55 60

Thr Thr Leu Leu Lys Ile Leu Ala Gly Lys His Met Val Gly Gly Arg

65 70 75 80

Asp Val Val Ser Val Leu Asn Gly Ser Ala Phe His Asp Thr Gln Leu

85 90 95

Val Cys Ser Gly Asp Leu Ala Tyr Leu Gly Ala Ser Trp Thr Lys Thr

100 105 110

Leu Gly Ser Ala Gly Glu Ile Pro Leu Gln Gly Asp Phe Ser Ala Glu

115 120 125

His Met Ile Phe Gly Val Glu Gly Ile Asp Pro Val Arg Arg Glu Lys

130 135 140

Leu Ile Glu Leu Leu Asp Ile Asp Leu Arg Trp Arg Met His Lys Val

145 150 155 160

Ser Asp Gly Gln Arg Arg Arg Val Gln Ile Cys Met Gly Leu Leu His

165 170 175

Pro Phe Gln Val Leu Leu Leu Asp Glu Val Thr Val Asp Leu Asp Val

180 185 190

Val Ala Arg Met Asp Leu Leu Asp Phe Phe Lys Glu Glu Cys Asp Gln

195 200 205

Arg Gly Ala Thr Ile Val Tyr Ala Thr His Ile Phe Asp Gly Leu Glu

210 215 220

Thr Trp Ala Thr His Leu Ala Tyr Ile Gln Asp Gly Gln Leu Arg Arg

225 230 235 240

Ala Glu Lys Leu Leu Glu Ile Asp Glu Leu Lys Ser Ser Val Asn Leu

245 250 255

Leu Ser Val Val Glu Ser Trp Leu Arg Ser Glu Thr Lys Cys Glu Lys

260 265 270

Lys Lys Leu Ile Asn Pro Thr Pro Gln Ile Gln Val Ser Pro Phe Gly

275 280 285

Thr Ser Pro Phe Met Pro Ser Arg His Met Ala Tyr Tyr Arg

290 295 300

<210> 77

<211> 329

<212> PRT

<213> 雷蒙德氏棉(Gossypium raimondii)

<220>

<221> MISC_FEATURE

<222> (1)..(329)

<223> ABC转运体

<400> 77

Met Ala Glu Gln Ala Cys Cys Arg Ser Gly Glu Gly Lys Glu Asn Gly

1 5 10 15

Gly Asp Ser Gln Gly Ile Thr Val Cys Gly Met Gln Phe Ala Tyr Glu

20 25 30

Leu Gln His Pro Leu Phe Phe Asp Phe Asn Leu Asp Ile Ala Arg Gly

35 40 45

Ser Arg Cys Leu Leu Val Gly Ala Asn Gly Ser Gly Lys Thr Thr Leu

50 55 60

Leu Lys Ile Leu Ala Gly Lys His Met Val Gly Gly Arg Glu Val Val

65 70 75 80

Arg Val Leu Asn Arg Ser Ala Phe His Asp Thr Gln Leu Val Cys Gly

85 90 95

Gly Asp Leu Ala Tyr Leu Gly Gly Ser Trp Ser Lys Thr Ile Gly Ser

100 105 110

Ala Val Ser Lys Phe Ser Tyr Phe Phe Phe Lys Ile Asn Ile His His

115 120 125

Phe Arg Leu Lys Leu Gln Ile Tyr Cys Ala Lys His Met Gln Gly Glu

130 135 140

Val Pro Leu Gln Gly Asp Phe Ser Ala Glu His Met Ile Phe Gly Val

145 150 155 160

Glu Gly Ile Asp Pro Val Arg Arg Asp Lys Leu Ile Glu Leu Leu Asp

165 170 175

Ile Asp Leu Gln Trp Arg Met His Lys Val Ser Asp Gly Gln Arg Arg

180 185 190

Arg Val Gln Ile Cys Met Gly Leu Leu His Pro Phe Gln Val Leu Leu

195 200 205

Leu Asp Glu Val Thr Val Asp Leu Asp Val Val Ala Arg Met Asp Leu

210 215 220

Leu Asp Phe Phe Lys Glu Glu Cys Glu Gln Arg Gly Ala Thr Val Val

225 230 235 240

Tyr Ala Thr His Ile Phe Asp Gly Leu Glu Thr Trp Ala Thr His Leu

245 250 255

Ala Tyr Ile Gln Asp Gly Gln Leu Lys Arg Ser Glu Lys Leu Thr Glu

260 265 270

Ile Asn Glu Leu Lys Ser Ser Glu Asn Leu Leu Ser Val Val Glu Ala

275 280 285

Trp Leu Arg Ser Glu Thr Lys Cys Glu Lys Lys Lys Pro Ser Asn Ser

290 295 300

Pro Ala Gln Val Gln Lys Ser Ser Pro Phe Gly Thr Ser Pro Phe Met

305 310 315 320

Ser Ser Arg His Met Ala Tyr Tyr Arg

325

<210> 78

<211> 282

<212> PRT

<213> 小豆(Vigna angularis)

<220>

<221> MISC_FEATURE

<222> (1)..(282)

<223> ABC转运体

<400> 78

Met Ala Ala Ala Glu Glu Asp Phe Pro Gly Ile Arg Val Arg Gly Met

1 5 10 15

Gln Phe Ser Tyr Glu Ala Gln Gln Pro Pro Leu Phe Leu Asp Phe Asn

20 25 30

Leu Asn Val Glu Pro Gly Ser Arg Cys Leu Leu Val Gly Ala Asn Gly

35 40 45

Ser Gly Lys Thr Thr Leu Leu Lys Ile Leu Ala Gly Lys His Met Val

50 55 60

Gly Gly Arg Asp Val Val Arg Val Leu Asn Gly Ser Ala Phe His Asp

65 70 75 80

Thr Gln Leu Val Cys Ser Gly Asp Leu Ala Tyr Leu Gly Gly Ser Trp

85 90 95

Ser Lys Asn Val Gly Ser Ala Gly Glu Ile Pro Leu Gln Gly Asp Phe

100 105 110

Ser Ala Glu His Met Ile Phe Gly Val Glu Gly Ala Asp Pro Asp Arg

115 120 125

Arg Asp Lys Leu Ile Glu Leu Leu Asp Ile Asp Leu Gln Trp Arg Met

130 135 140

His Lys Val Ser Asp Gly Gln Arg Arg Arg Val Gln Ile Cys Leu Gly

145 150 155 160

Leu Leu Tyr Pro Tyr Lys Val Leu Leu Leu Asp Glu Val Thr Val Asp

165 170 175

Leu Asp Val Val Thr Arg Met Asp Leu Leu Glu Phe Phe Arg Glu Glu

180 185 190

Cys Glu Gln Arg Glu Ala Thr Ile Val Tyr Ala Thr His Ile Phe Asp

195 200 205

Gly Leu Glu Ala Trp Ala Thr His Leu Ala Tyr Ile Gln Asp Gly Glu

210 215 220

Leu Arg Arg Ala Glu Lys Ile Ser Asp Val Asn Glu Leu Lys Ser Ser

225 230 235 240

Thr Asn Leu Leu Ser Val Val Glu Asp Trp Leu Arg Ala Glu Thr Lys

245 250 255

Gly Val Lys Lys Lys Pro Val Gln Lys Asn Phe Ala Ser Ser Pro Phe

260 265 270

Phe Ser Ser Arg His Met Ala Tyr Tyr Arg

275 280

<210> 79

<211> 286

<212> PRT

<213> 渐狭叶烟草(Nicotiana attenuate)

<220>

<221> MISC_FEATURE

<222> (1)..(286)

<223> ABC转运体

<400> 79

Met Glu Gly Arg Ser Asn Ser Ile Leu Val Asn Gly Met Gln Phe Ser

1 5 10 15

Tyr Asp Phe Gln Ser Pro Ile Phe Phe Asp Phe Ser Leu Lys Ile Ser

20 25 30

Pro Gly Ser Arg Cys Leu Leu Val Gly Ala Asn Gly Ser Gly Lys Thr

35 40 45

Thr Leu Leu Arg Ile Leu Ala Gly Lys His Met Val Gly Gly Arg Asp

50 55 60

Val Val Lys Val Leu Asn Phe Ser Ala Phe His Asp Thr His Leu Val

65 70 75 80

Cys Asp Gly Asp Leu Ala Tyr Leu Gly Glu Ser Trp Ser Lys Asn Ala

85 90 95

Gly Ser Ala Gly Glu Ile Pro Leu Gln Gly Asp Phe Ser Ala Glu His

100 105 110

Met Ile Phe Gly Val Glu Gly Val Asp Pro Val Arg Arg Glu Lys Leu

115 120 125

Ile Glu Leu Leu Asp Ile Asn Leu Gln Trp Arg Met His Lys Val Ser

130 135 140

Asp Gly Gln Arg Arg Arg Val Gln Ile Cys Met Gly Leu Leu His Pro

145 150 155 160

Tyr Lys Val Leu Leu Leu Asp Glu Val Thr Val Asp Leu Asp Val Val

165 170 175

Ala Arg Met Asp Leu Leu Asp Phe Phe Lys Glu Glu Cys Glu Gln Arg

180 185 190

Gly Ala Thr Ile Val Tyr Ala Thr His Ile Phe Asp Gly Leu Glu Ser

195 200 205

Trp Ala Thr Asp Leu Val Tyr Ile Gln Asp Gly Val Leu Lys Arg Ser

210 215 220

Glu Lys Leu Pro Glu Leu Pro Glu Leu Lys Ser Ser Pro Asn Leu Leu

225 230 235 240

Ser Val Val Glu Asn Trp Leu Arg Ser Glu Thr Thr Ile Glu Lys Lys

245 250 255

Lys Pro Val Ile Ala Pro Pro Lys Val Gln Lys Ser Ser Pro Phe Gly

260 265 270

Ser Ser Pro Phe Gln Ser Ser Arg His Met Ala Tyr Phe Arg

275 280 285

<210> 80

<211> 307

<212> PRT

<213> 甜瓜(Cucumis melo)

<220>

<221> MISC_FEATURE

<222> (1)..(307)

<223> ABC转运体

<400> 80

Met Ala Asp Asn Gly Ile Ser Leu Val Gly Asn Asp Gly Ile Glu Val

1 5 10 15

Val Asp Glu Lys Gly Ser Pro Gly Ile Lys Val Gln Ala Met Gln Phe

20 25 30

Ser Tyr Glu Ser Asp Ser Pro Leu Phe Val Glu Phe Asn Leu Gln Ile

35 40 45

Gly Ser Gly Ser Arg Cys Leu Leu Val Gly Ala Asn Gly Ser Gly Lys

50 55 60

Thr Thr Leu Leu Lys Ile Leu Ala Gly Lys His Met Val Gly Gly Arg

65 70 75 80

Asp Val Val Gln Val Leu Asn Gly Ser Ala Phe His Asp Thr Gln Leu

85 90 95

Val Cys Ser Gly Asp Leu Ala Tyr Leu Gly Gly Ser Trp Ser Lys Thr

100 105 110

Val Ser Ser Ala Gly Glu Val Ala Leu Gln Gly Asp Phe Ser Ala Glu

115 120 125

His Met Ile Phe Gly Val Glu Gly Thr Asp Pro Glu Arg Arg Glu Arg

130 135 140

Leu Ile Asp Leu Leu Asp Ile Asp Leu Arg Trp Arg Met His Lys Val

145 150 155 160

Ser Asp Gly Gln Arg Arg Arg Val Gln Ile Cys Met Gly Leu Leu His

165 170 175

Pro Phe Lys Val Leu Leu Leu Asp Glu Val Thr Val Asp Leu Asp Val

180 185 190

Val Ala Arg Met Asp Leu Leu Asp Phe Leu Lys Glu Glu Cys Asp Gln

195 200 205

Arg Gly Ala Thr Ile Val Tyr Ala Thr His Ile Phe Asp Gly Leu Glu

210 215 220

Thr Trp Ala Thr His Leu Ala Tyr Ile Gln Asp Gly Glu Leu Arg Lys

225 230 235 240

Ser Glu Lys Leu Ser Ala Val Glu Glu Leu Lys Thr Cys Ala Asn Leu

245 250 255

Leu Ser Val Val Glu Thr Trp Leu Arg Ala Glu Thr Lys Leu Glu Lys

260 265 270

Lys Lys Lys Lys Gln Pro Ile Gln Pro Pro Ser Asn Asn Gln Lys Ile

275 280 285

Val Ser Pro Phe Gly Ser Ser Pro Phe Thr Ser Ser Arg His Met Ala

290 295 300

Tyr Tyr Arg

305

<210> 81

<211> 289

<212> PRT

<213> 狭叶羽扇豆(Lupinus angustifolius)

<220>

<221> MISC_FEATURE

<222> (1)..(289)

<223> ABC转运体

<400> 81

Met Ala Arg Glu Ser Glu Asn Gly Ile Val Val Lys Ala Met Gln Phe

1 5 10 15

Ser Tyr Glu Tyr Ser Glu Lys Glu Pro Pro Leu Phe Ile Asp Phe Asn

20 25 30

Leu Asn Val Ser Pro Gly Ser Arg Cys Leu Leu Leu Gly Ala Asn Gly

35 40 45

Ser Gly Lys Thr Thr Leu Leu Lys Ile Leu Ala Gly Lys His Met Val

50 55 60

Gly Gly Arg Asp Val Val Arg Val Leu Asn Gly Ser Ala Phe His Asp

65 70 75 80

Thr Lys Leu Val Cys Ser Gly Asp Leu Ala Tyr Leu Gly Gly Ser Trp

85 90 95

Ser Lys Asn Val Ser Ser Ala Gly Asp Ile Pro Leu Gln Gly Asp Phe

100 105 110

Ser Ala Glu His Met Ile Phe Gly Val Glu Gly Ala Asp Pro Asp Arg

115 120 125

Arg Asp Lys Leu Ile Asp Leu Leu Asp Ile Asp Leu Asn Trp Arg Met

130 135 140

His Lys Val Ser Asp Gly Gln Arg Arg Arg Val Gln Ile Cys Leu Gly

145 150 155 160

Leu Leu His Pro Tyr Lys Val Leu Leu Leu Asp Glu Val Thr Val Asp

165 170 175

Leu Asp Val Val Thr Arg Met Asp Leu Leu Asp Phe Phe Lys Glu Glu

180 185 190

Cys Glu Gln Arg Glu Ala Thr Ile Val Tyr Ala Thr His Ile Phe Asp

195 200 205

Gly Leu Glu Thr Trp Ala Thr His Leu Ala Tyr Ile Gln Glu Gly Glu

210 215 220

Leu Arg Arg Ala Glu Lys Ile Ser Asp Val Ser Glu Leu Lys Ser Ser

225 230 235 240

Thr Asn Leu Leu Ser Ile Val Glu Ser Trp Leu Arg Ala Glu Thr Lys

245 250 255

Leu Glu Lys Lys Lys Pro Val His Lys Asp Ser His Thr Gln Asn Thr

260 265 270

Ser Phe Val Ser Ser Pro Phe Phe Ser Ser Arg His Met Ala Tyr Tyr

275 280 285

Arg

<210> 82

<211> 291

<212> PRT

<213> 白菜型油菜(Brassica rapa)

<220>

<221> MISC_FEATURE

<222> (1)..(291)

<223> ABC转运体

<400> 82

Met Ala Ala Lys Asp Gln Ala Thr Thr Ser Asp Asp Ala Ile Arg Val

1 5 10 15

Ser Gly Met Glu Phe Ser Tyr Glu Ala Glu Asp Pro Ile Phe Phe Asp

20 25 30

Phe Asn Leu Asp Leu Pro Ala Gly Ser Arg Cys Leu Leu Val Gly Ala

35 40 45

Asn Gly Ser Gly Lys Thr Thr Leu Leu Lys Ile Leu Ala Gly Lys His

50 55 60

Met Val Gly Gly Lys Asn Val Val Gln Val Leu Ser Arg Ser Ala Phe

65 70 75 80

His Asp Thr Gln Leu Val Cys Ser Gly Asp Leu Ser Tyr Leu Gly Gly

85 90 95

Ser Trp Ser Lys Thr Val Gly Ser Ala Gly Glu Val Pro Leu Gln Gly

100 105 110

Asp Phe Ser Ala Glu His Met Ile Phe Gly Val Glu Gly Ile Asp Pro

115 120 125

Val Arg Arg Glu Lys Leu Ile Asp Leu Leu Asp Ile Asn Leu Gln Trp

130 135 140

Arg Met His Lys Val Ser Asp Gly Gln Lys Arg Arg Val Gln Ile Cys

145 150 155 160

Met Gly Leu Leu His Pro Phe Lys Val Leu Leu Leu Asp Glu Val Thr

165 170 175

Val Asp Leu Asp Val Val Ala Arg Met Asp Leu Leu Glu Phe Phe Lys

180 185 190

Glu Glu Cys Asp Gln Arg Gly Ala Thr Ile Val Tyr Ala Thr His Ile

195 200 205

Phe Asp Gly Leu Glu Thr Trp Ala Thr His Leu Ala Tyr Ile Gln Asn

210 215 220

Gly Glu Leu Asn Arg Ser Ser Lys Met Ala Asp Ile Ser Glu Met Lys

225 230 235 240

Thr Ser Pro Asn Leu Leu Ser Val Val Glu Ser Trp Leu Arg Ser Glu

245 250 255

Thr Lys Ile Asp Lys Lys Lys Lys Lys Glu Pro Val Pro Ala Trp Lys

260 265 270

Pro Thr Pro Phe Asp Asn Ser Pro Phe Arg Ser Ser Arg His Met Ala

275 280 285

Tyr Tyr Arg

290

<210> 83

<211> 302

<212> PRT

<213> 莲(Nelumbo nucifera)

<220>

<221> MISC_FEATURE

<222> (1)..(302)

<223> ABC转运体

<400> 83

Met Lys Lys Gln Asn Met Val Met Gly Lys Glu Glu Leu Gln Ser Arg

1 5 10 15

Glu Val Glu Glu Ser Ser Cys Ile Glu Val Ser Ala Leu His Phe Ala

20 25 30

Tyr Asp Gly Gln Pro Pro Leu Phe Ala Asn Phe Asn Leu Arg Ile Ser

35 40 45

Arg Gly Ser Arg Cys Leu Leu Val Gly Ala Asn Gly Ser Gly Lys Thr

50 55 60

Thr Leu Leu Lys Ile Leu Ala Gly Lys His Met Val Gly Gly Arg Asp

65 70 75 80

Val Val Arg Val Leu Asn Cys Ser Ala Phe His Asp Thr His Leu Val

85 90 95

Cys Ser Gly Asp Leu Ala Tyr Leu Gly Gly Ser Trp Ser Lys Asn Ile

100 105 110

Gly Ser Ala Gly Glu Ile Pro Leu Gln Gly Asp Phe Ser Ala Glu His

115 120 125

Met Ile Phe Gly Val Glu Gly Thr Asp Pro Val Arg Arg Gln Lys Leu

130 135 140

Ile Glu Leu Leu Asp Ile Asp Leu Gln Trp Arg Met His Lys Val Ser

145 150 155 160

Asp Gly Gln Arg Arg Arg Val Gln Ile Cys Met Gly Leu Leu His Leu

165 170 175

Phe Gln Val Leu Leu Leu Asp Glu Val Thr Val Asp Leu Asp Val Val

180 185 190

Ala Arg Leu Asp Leu Leu Glu Phe Leu Lys Glu Glu Cys Asp Gln Arg

195 200 205

Gly Ala Thr Ile Val Tyr Ala Thr His Ile Phe Asp Gly Leu Glu Thr

210 215 220

Trp Ala Thr Asp Val Ala Tyr Val Gln Asp Gly Asp Leu Lys Lys Ala

225 230 235 240

Glu Lys Leu Ser Glu Leu Gln Glu Leu Lys Ser Ala Ser Asn Leu Leu

245 250 255

Ser Val Val Glu Ser Trp Leu Arg Ser Glu Thr Lys Cys Glu Cys Lys

260 265 270

Lys Val Leu Asn Pro Pro Ala Glu Thr Arg Lys Thr Ser Pro Phe Asp

275 280 285

Asn Ser Pro Phe Arg Ser Ser Arg His Met Ala Tyr Tyr Arg

290 295 300

<210> 84

<211> 292

<212> PRT

<213> 萝卜(Raphanus sativus)

<220>

<221> MISC_FEATURE

<222> (1)..(292)

<223> ABC转运体

<400> 84

Met Ala Ala Lys Asp Pro Ala Thr Thr Ser Asp Asp Ala Ile Arg Val

1 5 10 15

Ser Gly Met Glu Phe Cys Tyr Glu Ala Asp Asp Pro Ile Phe Phe Asp

20 25 30

Phe Asn Leu Glu Leu Pro Ala Gly Ser Arg Cys Leu Leu Val Gly Ala

35 40 45

Asn Gly Ser Gly Lys Thr Thr Leu Leu Lys Ile Leu Ala Gly Lys His

50 55 60

Met Val Gly Gly Lys Asn Val Val Gln Val Leu Ser Arg Ser Ala Phe

65 70 75 80

His Asp Thr Gln Leu Ile Cys Ser Gly Asp Leu Ser Tyr Leu Gly Gly

85 90 95

Ser Trp Ser Lys Thr Val Gly Ser Ala Gly Glu Val Pro Leu Gln Gly

100 105 110

Asp Phe Ser Ala Glu His Met Ile Phe Gly Val Glu Gly Ile Asp Pro

115 120 125

Val Arg Arg Glu Lys Leu Ile Asp Leu Leu Asp Ile Asn Leu Gln Trp

130 135 140

Arg Met His Lys Val Ser Asp Gly Gln Lys Arg Arg Val Gln Ile Cys

145 150 155 160

Met Gly Leu Leu His Pro Phe Lys Val Leu Leu Leu Asp Glu Val Thr

165 170 175

Val Asp Leu Asp Val Val Ala Arg Met Asp Leu Leu Glu Phe Phe Lys

180 185 190

Glu Glu Cys Asp Gln Arg Gly Ala Thr Ile Val Tyr Ala Thr His Ile

195 200 205

Phe Asp Gly Leu Glu Thr Trp Ala Thr His Leu Ala Tyr Ile Gln Asn

210 215 220

Gly Glu Leu Asn Arg Ser Ser Lys Met Ala Asp Ile Asn Glu Met Lys

225 230 235 240

Thr Ser Pro Asn Leu Leu Ser Val Val Glu Ser Trp Leu Arg Ser Glu

245 250 255

Thr Lys Leu Val Lys Lys Lys Lys Lys Lys Glu Pro Val Ala Ala Trp

260 265 270

Lys Pro Ser Pro Phe Asp Asn Ser Pro Phe Arg Ser Ser Arg His Met

275 280 285

Ala Tyr Tyr Arg

290

<210> 85

<211> 291

<212> PRT

<213> 大叶藻(Zostera marina)

<220>

<221> MISC_FEATURE

<222> (1)..(291)

<223> ABC转运体

<400> 85

Met Lys Ser Met Val Gly Asn Gly Gly Ile Glu Val Ser Cys Met Gln

1 5 10 15

Phe Gly Tyr Asp Gly Ser His Thr Pro Leu Phe Ala Lys Phe Asp Leu

20 25 30

Gln Ile Pro Pro Gly Ser Arg Cys Leu Leu Val Gly Ala Asn Gly Ser

35 40 45

Gly Lys Thr Thr Leu Leu Lys Ile Leu Ala Gly Lys His Met Val Gly

50 55 60

Gly Gln Asp Val Val Lys Ile Leu Asp Ser Ser Ala Phe His Asp Thr

65 70 75 80

Gln Leu Val Cys Ser Gly Asp Leu Ala Tyr Leu Gly His Ser Trp Ser

85 90 95

Arg Ser Ile Gly Ala Ala Gly Asp Val Pro Leu Gln Gly Asp Phe Ser

100 105 110

Ala Glu His Met Ile Phe Gly Val Gln Gly Ile Asp Asn Ser Arg Arg

115 120 125

Glu Lys Leu Ile Asp Leu Leu Asp Ile Asp Leu Gln Trp Arg Met His

130 135 140

Asn Val Ser Asp Gly Gln Arg Arg Arg Val Gln Ile Cys Leu Gly Leu

145 150 155 160

Leu His Pro Tyr Lys Val Leu Leu Leu Asp Glu Val Thr Val Asp Leu

165 170 175

Asp Val Val Thr Arg Met Asp Leu Leu Gly Phe Leu Lys Glu Glu Cys

180 185 190

Glu Glu Arg Gly Ala Ile Ile Val Tyr Ala Thr His Ile Phe Asp Gly

195 200 205

Leu Glu Thr Trp Ala Ser Asp Val Ala Tyr Ile Gln Gln Gly Val Leu

210 215 220

Lys Lys Cys Ala Lys Met Ser Gln Ile His Glu Leu Gly Thr Cys Lys

225 230 235 240

Asn Leu Leu Ser Val Val Glu Ser Trp Leu Arg Ala Glu Thr Lys Ile

245 250 255

Leu Lys Lys Glu Glu Ser Ser Thr Val Val His Leu Gln Leu Gln Thr

260 265 270

Pro Thr Val Val Ala Pro Ser Pro Phe Arg Ala Ser Arg His Met Ala

275 280 285

Tyr Tyr Arg

290

<210> 86

<211> 299

<212> PRT

<213> 木豆(Cajanus cajan)

<220>

<221> MISC_FEATURE

<222> (1)..(299)

<223> ABC转运体

<400> 86

Met Asn Lys Ser Leu Ser Asn Gly Gly Cys Thr Arg Thr Leu Thr Thr

1 5 10 15

Met Ala Pro Glu Gln Glu Asp Ser Arg Gly Ile Arg Val Asn Ala Met

20 25 30

Gln Phe Ser Tyr Asp Val Gln Gln Pro Pro Leu Phe Leu Asp Phe Ser

35 40 45

Leu Asp Val Glu Pro Gly Ser Arg Cys Leu Leu Val Gly Ala Asn Gly

50 55 60

Ser Gly Lys Thr Thr Leu Leu Lys Ile Leu Ala Gly Lys His Met Val

65 70 75 80

Gly Gly Arg Asp Val Val Arg Val Leu Asn Gly Ser Ala Phe His Asp

85 90 95

Thr Gln Leu Val Cys Ser Gly Asp Leu Ala Tyr Leu Gly Gly Ser Trp

100 105 110

Ser Lys Asn Val Gly Ser Ala Gly Glu Ile Pro Leu Gln Gly Asp Phe

115 120 125

Ser Ala Glu His Met Ile Phe Gly Val Glu Gly Ala Asp Pro Asp Arg

130 135 140

Arg Asp Lys Leu Ile Asp Leu Leu Asp Ile Asp Leu Gln Trp Arg Met

145 150 155 160

His Lys Val Ser Asp Gly Gln Arg Arg Arg Val Gln Ile Cys Leu Gly

165 170 175

Leu Leu Tyr Pro Tyr Lys Val Leu Leu Leu Asp Glu Val Thr Val Asp

180 185 190

Leu Asp Val Val Thr Arg Met Asp Leu Leu Asp Phe Phe Arg Glu Glu

195 200 205

Cys Glu Gln Arg Glu Ala Thr Ile Val Tyr Ala Thr His Ile Phe Asp

210 215 220

Gly Leu Glu Thr Trp Ala Thr His Leu Ala Tyr Ile Gln Asp Gly Glu

225 230 235 240

Leu Arg Arg Ala Glu Lys Leu Ser Asp Val Asn Glu Leu Lys Ser Thr

245 250 255

Thr Asn Leu Leu Ser Val Val Glu Ser Trp Leu Arg Ala Glu Thr Lys

260 265 270

Leu Glu Lys Lys Asn Pro Val Gln Lys Thr Ser Phe Ala Ser Ser Pro

275 280 285

Phe Phe Ser Ser Arg His Met Ala Tyr Tyr Arg

290 295

<210> 87

<211> 305

<212> PRT

<213> 大桉(Eucalyptus grandis)

<220>

<221> MISC_FEATURE

<222> (1)..(305)

<223> ABC转运体

<400> 87

Met Val Gly Val Gly Asp Val Ala Lys Pro Arg Gly Glu Ala Gly Gly

1 5 10 15

Gly Asp Gly Asp Gly Gly Glu Arg Asp Ser Val Arg Val Cys Gly Met

20 25 30

Gln Phe Ala Tyr Glu Gly Gln Pro Pro Leu Phe Leu Asp Phe Asn Leu

35 40 45

Ser Val Ser Pro Gly Ser Arg Cys Leu Leu Leu Gly Ala Asn Gly Ser

50 55 60

Gly Lys Thr Thr Leu Leu Lys Ile Leu Ala Gly Lys Asn Met Val Gly

65 70 75 80

Gly Gln Asn Val Val Arg Val Leu Asn Arg Ser Ala Phe His Asn Thr

85 90 95

Gln Leu Val Cys Ser Gly Asp Leu Ala Tyr Leu Gly Gly Ser Trp Ser

100 105 110

Lys Thr Val Gly Ser Ala Gly Glu Ile Pro Leu Gln Gly Asp Phe Ser

115 120 125

Ala Glu His Met Ile Phe Gly Val Glu Gly Ala Asp Pro Val Arg Arg

130 135 140

Glu Lys Leu Ile Glu Leu Leu Asp Ile Asp Leu Gln Trp Arg Met His

145 150 155 160

Lys Val Ser Asp Gly Gln Arg Arg Arg Val Gln Ile Cys Met Gly Leu

165 170 175

Leu His Pro Phe Gln Val Leu Leu Leu Asp Glu Val Thr Val Asp Leu

180 185 190

Asp Val Val Thr Arg Met Asp Phe Leu Asp Phe Leu Arg Glu Glu Cys

195 200 205

Asp Gln Arg Gly Ala Ala Ile Val Tyr Ala Thr His Ile Phe Asp Gly

210 215 220

Leu Glu Thr Trp Ala Thr His Leu Val Tyr Ile Gln Asp Gly Glu Leu

225 230 235 240

Arg Arg Ala Glu Arg Leu Thr Glu Val Asp Asp Leu Lys Ser Ser Ala

245 250 255

Asn Leu His Ser Val Val Glu Ala Trp Leu Arg Ser Glu Thr Lys Arg

260 265 270

Glu Lys Lys Lys Pro Val Thr Thr Ser Ser Gln Pro Gln Arg Thr Ser

275 280 285

Leu Ser Gly Ala Ser Pro Phe Thr Ser Ser Arg His Met Ala Tyr Tyr

290 295 300

Arg

305

<210> 88

<211> 291

<212> PRT

<213> 甘蓝(Brassica oleracea)

<220>

<221> MISC_FEATURE

<222> (1)..(291)

<223> ABC转运体

<400> 88

Met Ala Ala Lys Asp Gln Ala Thr Thr Ser Asp Asp Ala Ile Arg Val

1 5 10 15

Ser Gly Met Glu Phe Ser Tyr Glu Ala Glu Asp Pro Ile Phe Phe Asp

20 25 30

Phe Asn Leu Asp Leu Pro Ala Gly Ser Arg Cys Leu Leu Val Gly Ala

35 40 45

Asn Gly Ser Gly Lys Thr Thr Leu Leu Lys Ile Leu Ala Gly Lys His

50 55 60

Met Val Gly Gly Lys Asn Val Val Gln Val Leu Ser Arg Ser Ala Phe

65 70 75 80

His Asp Thr Gln Leu Val Cys Ser Gly Asp Leu Ser Tyr Leu Gly Gly

85 90 95

Ser Trp Ser Lys Thr Ile Gly Ser Ala Gly Glu Val Pro Leu Gln Gly

100 105 110

Asp Phe Ser Ala Glu His Met Ile Phe Gly Val Glu Gly Ile Asp Pro

115 120 125

Val Arg Arg Asp Lys Leu Ile Asp Leu Leu Asp Ile Asn Leu Gln Trp

130 135 140

Arg Met His Lys Val Ser Asp Gly Gln Lys Arg Arg Val Gln Ile Cys

145 150 155 160

Met Gly Leu Leu His Pro Phe Lys Val Leu Leu Leu Asp Glu Val Thr

165 170 175

Val Asp Leu Asp Val Val Ala Arg Met Asp Leu Leu Glu Phe Phe Lys

180 185 190

Glu Glu Cys Asp Gln Arg Gly Ala Thr Ile Val Tyr Ala Thr His Ile

195 200 205

Phe Asp Gly Leu Glu Thr Trp Ala Thr His Leu Ala Tyr Ile Gln Asn

210 215 220

Gly Glu Leu Asn Arg Ser Ser Lys Met Ala Asp Ile Ser Glu Met Lys

225 230 235 240

Thr Ser Pro Asn Leu Leu Ser Val Val Glu Ser Trp Leu Arg Ser Glu

245 250 255

Thr Lys Ile Tyr Lys Lys Lys Lys Lys Glu Pro Val Ala Ala Trp Lys

260 265 270

Pro Thr Pro Phe Asp Asn Ser Pro Phe Arg Ser Ser Arg His Met Ala

275 280 285

Tyr Tyr Arg

290

<210> 89

<211> 288

<212> PRT

<213> 猴面花(Erythranthe guttata)

<220>

<221> MISC_FEATURE

<222> (1)..(288)

<223> ABC转运体

<400> 89

Met Gly Glu Glu Ser His Ser Ile Gln Val Ser Gly Met Gln Phe Ser

1 5 10 15

Tyr Asp Ala Ile Arg Ser Pro Leu Phe Phe Asp Phe Asn Leu Asp Ile

20 25 30

Ser Pro Arg Ser Arg Cys Leu Leu Val Gly Ala Asn Gly Ser Gly Lys

35 40 45

Thr Thr Leu Leu Arg Ile Leu Ala Gly Lys His Met Val Gly Gly Arg

50 55 60

Asp Val Val Arg Val Leu Asn Cys Ser Ala Phe His Asp Thr Ser Leu

65 70 75 80

Val Cys Asn Gly Asp Leu Ala Tyr Leu Gly Glu Ser Trp Ser Lys Thr

85 90 95

Val Gly Ser Ala Gly Glu Leu Pro Leu Gln Gly Asp Phe Ser Ala Glu

100 105 110

His Met Ile Phe Gly Val Glu Gly Val Asp Pro Val Arg Arg Asp Lys

115 120 125

Leu Ile Glu Leu Leu Asp Ile Asp Leu Arg Trp Arg Met His Lys Val

130 135 140

Ser Asp Gly Gln Arg Arg Arg Val Gln Ile Cys Met Gly Leu Leu His

145 150 155 160

Pro Tyr Lys Val Leu Leu Leu Asp Glu Val Thr Val Asp Leu Asp Val

165 170 175

Val Ala Arg Met Asp Leu Leu Glu Phe Phe Lys Glu Glu Cys Glu Gln

180 185 190

Arg Gly Ala Thr Ile Val Tyr Ala Thr His Ile Phe Asp Gly Leu Glu

195 200 205

Thr Trp Ala Thr Asp Leu Val Tyr Val Gln Asp Gly Val Leu Asn Lys

210 215 220

Ser Gln Lys Leu Ser Asp Leu Asn Glu Leu Lys Asn Asn Leu Asn Leu

225 230 235 240

Leu Ser Val Val Glu Ser Trp Leu Arg Ser Glu Thr Lys Ile Glu Lys

245 250 255

Lys Lys Thr Gln Val Asn Ser Thr Pro Gln Pro Lys Lys Ser Ser Pro

260 265 270

Phe Asp Ser Ser Pro Phe Arg Ser Ser Arg His Met Ala Tyr Tyr Arg

275 280 285

<210> 90

<211> 282

<212> PRT

<213> 绿豆(Vigna radiata)

<220>

<221> MISC_FEATURE

<222> (1)..(282)

<223> ABC转运体

<400> 90

Met Ala Ala Ala Glu Glu Asp Ser Pro Gly Ile Arg Val Ser Gly Met

1 5 10 15

Gln Phe Ser Tyr Glu Ala Gln Gln Pro Pro Leu Phe Leu Asp Phe Asn

20 25 30

Leu Asn Val Lys Pro Gly Ser Arg Cys Leu Leu Val Gly Ala Asn Gly

35 40 45

Ser Gly Lys Thr Thr Leu Leu Lys Ile Leu Ala Gly Lys His Met Val

50 55 60

Gly Gly Arg Asp Val Val Arg Val Leu Asn Gly Ser Ala Phe His Asp

65 70 75 80

Thr Gln Leu Val Cys Ser Gly Asp Leu Ala Tyr Leu Gly Gly Ser Trp

85 90 95

Ser Lys Asn Val Gly Ser Ala Gly Glu Ile Pro Leu Gln Gly Asp Phe

100 105 110

Ser Ala Glu His Met Ile Phe Gly Val Glu Gly Ala Asp Pro Asp Arg

115 120 125

Arg Asp Lys Leu Ile Glu Leu Leu Asp Ile Asp Leu Gln Trp Arg Met

130 135 140

His Lys Val Ser Asp Gly Gln Arg Arg Arg Val Gln Ile Cys Leu Gly

145 150 155 160

Leu Leu Tyr Pro Tyr Lys Val Leu Leu Leu Asp Glu Val Thr Val Asp

165 170 175

Leu Asp Val Val Thr Arg Met Asp Leu Leu Glu Phe Phe Arg Glu Glu

180 185 190

Cys Glu Gln Arg Glu Ala Thr Ile Val Tyr Ala Thr His Ile Phe Asp

195 200 205

Gly Leu Glu Ala Trp Ala Thr His Leu Ala Tyr Ile Gln Asp Gly Glu

210 215 220

Leu Arg Arg Ala Glu Lys Ile Ser Asp Val Asn Glu Leu Lys Ser Ser

225 230 235 240

Thr Asn Leu Leu Ser Val Val Glu Thr Trp Leu Arg Ala Glu Thr Lys

245 250 255

Val Val Lys Lys Lys Pro Val Gln Lys Asn Phe Ala Ser Ser Pro Phe

260 265 270

Phe Ser Ser Arg His Met Ala Tyr Tyr Arg

275 280

<210> 91

<211> 286

<212> PRT

<213> 辣椒(Capsicum annuum)

<220>

<221> MISC_FEATURE

<222> (1)..(286)

<223> ABC转运体

<400> 91

Met Glu Gly Glu Ser Ser Ser Ile Arg Val Asn Gly Met Gln Phe Ser

1 5 10 15

Tyr Asp Phe Gln Ser Pro Ile Phe Phe Asp Phe Asn Leu Asn Ile Ser

20 25 30

Pro Arg Ser Arg Cys Leu Leu Leu Gly Ala Asn Gly Ser Gly Lys Thr

35 40 45

Thr Leu Leu Lys Ile Leu Ala Gly Lys His Met Val Gly Gly Lys Glu

50 55 60

Val Val Arg Val Leu Asn Phe Ser Ala Phe His Asp Thr His Leu Val

65 70 75 80

Cys Ser Gly Asp Leu Ala Tyr Leu Gly Glu Ser Trp Ser Lys Thr Val

85 90 95

Gly Thr Val Gly Glu Val Pro Leu Gln Gly Asp Phe Ser Ala Glu His

100 105 110

Met Ile Phe Gly Val Glu Gly Val Asp Pro Val Arg Arg Glu Lys Leu

115 120 125

Ile Glu Leu Leu Asp Ile Asp Leu Gln Trp Arg Met His Lys Val Ser

130 135 140

Asp Gly Gln Arg Arg Arg Val Gln Ile Cys Met Gly Leu Leu His Pro

145 150 155 160

Tyr Lys Val Leu Leu Leu Asp Glu Val Thr Val Asp Leu Asp Val Val

165 170 175

Ala Arg Met Asp Leu Leu Asp Phe Phe Lys Glu Glu Cys Glu Gln Arg

180 185 190

Gly Ala Thr Ile Val Tyr Ala Thr His Ile Phe Asp Gly Leu Glu Thr

195 200 205

Trp Ala Thr Asp Leu Val Tyr Ile Gln Asp Gly Val Leu Lys Arg Ser

210 215 220

Glu Lys Leu Pro Glu Leu Pro Glu Leu Lys Ser Ala Pro Asn Leu Leu

225 230 235 240

Ser Val Val Glu Asn Trp Leu Arg Ser Glu Thr Thr Ile Glu Lys Lys

245 250 255

Lys Pro Leu Pro Ala Pro Pro Lys Val Gln Lys Ser Ser Pro Phe Val

260 265 270

Ser Ser Pro Phe Gln Ser Ser Arg His Met Ala Tyr Phe Arg

275 280 285

<210> 92

<211> 286

<212> PRT

<213> 潘那利番茄(Solanum pennellii)

<220>

<221> MISC_FEATURE

<222> (1)..(286)

<223> ABC转运体

<400> 92

Met Ala Gly Glu Ser Ser Ser Ile Gln Val Asn Gly Met Gln Phe Ser

1 5 10 15

Tyr Asp Phe Gln Ser Pro Ile Tyr Phe Asp Phe Asn Leu Ser Val Ala

20 25 30

Pro Arg Ser Arg Cys Leu Leu Leu Gly Ala Asn Gly Ser Gly Lys Thr

35 40 45

Thr Leu Leu Lys Ile Leu Ala Gly Lys His Met Val Gly Gly Lys Asp

50 55 60

Val Val Arg Val Leu Asn Leu Ser Ala Phe His Asp Thr His Leu Val

65 70 75 80

Cys Ser Gly Asp Leu Ala Tyr Leu Gly Glu Ser Trp Ser Lys Asn Val

85 90 95

Gly Ala Val Gly Asp Ile Pro Leu Gln Gly Asp Phe Ser Ala Glu His

100 105 110

Met Ile Phe Gly Val Glu Gly Val Asp Pro Val Arg Arg Glu Lys Leu

115 120 125

Ile Glu Leu Leu Asp Ile Asp Leu Gln Trp Arg Met His Lys Val Ser

130 135 140

Asp Gly Gln Arg Arg Arg Val Gln Ile Cys Met Gly Leu Leu His Pro

145 150 155 160

Tyr Lys Val Leu Leu Met Asp Glu Val Thr Val Asp Leu Asp Val Val

165 170 175

Ala Arg Met Asp Leu Leu Asp Phe Phe Lys Glu Glu Cys Glu Gln Arg

180 185 190

Gly Ala Thr Ile Val Tyr Ala Thr His Ile Phe Asp Gly Leu Glu Thr

195 200 205

Trp Ala Thr Asp Leu Val Tyr Ile Gln Glu Gly Val Leu Lys Arg Thr

210 215 220

Glu Lys Leu Pro Glu Leu Pro Glu Leu Lys Ser Ser Pro Asn Leu Leu

225 230 235 240

Ser Val Val Glu Asn Trp Leu Arg Ser Glu Thr Pro Ile Glu Lys Lys

245 250 255

Lys Pro Ala Pro Ala Pro Ala Lys Ala Gln Lys Ser Ser Pro Phe Gly

260 265 270

Ser Ser Pro Phe Gln Ser Ser Arg His Met Ser His Tyr Arg

275 280 285

<210> 93

<211> 286

<212> PRT

<213> 番茄(Solanum lycopersicum)

<220>

<221> MISC_FEATURE

<222> (1)..(286)

<223> ABC转运体

<400> 93

Met Ala Gly Glu Ser Ser Ser Ile Gln Val Asn Gly Met Gln Phe Ser

1 5 10 15

Tyr Asp Phe Gln Ser Pro Ile Tyr Phe Asp Phe Asn Leu Asn Val Ala

20 25 30

Pro Arg Ser Arg Cys Leu Leu Leu Gly Ala Asn Gly Ser Gly Lys Thr

35 40 45

Thr Leu Leu Lys Ile Leu Ala Gly Lys His Met Val Gly Gly Lys Asp

50 55 60

Val Val Arg Val Leu Asn Leu Ser Ala Phe His Asp Thr His Leu Val

65 70 75 80

Cys Ser Gly Asp Leu Ala Tyr Leu Gly Glu Ser Trp Ser Lys Asn Val

85 90 95

Gly Ala Val Gly Asp Ile Pro Leu Gln Gly Asp Phe Ser Ala Glu His

100 105 110

Met Ile Phe Gly Val Glu Gly Val Asp Pro Val Arg Arg Glu Lys Leu

115 120 125

Ile Glu Leu Leu Asp Ile Asp Leu Gln Trp Arg Met His Lys Val Ser

130 135 140

Asp Gly Gln Arg Arg Arg Val Gln Ile Cys Met Gly Leu Leu His Pro

145 150 155 160

Tyr Lys Val Leu Leu Met Asp Glu Val Thr Val Asp Leu Asp Val Val

165 170 175

Ala Arg Met Asp Leu Leu Asp Phe Phe Lys Glu Glu Cys Glu Gln Arg

180 185 190

Gly Ala Thr Ile Val Tyr Ala Thr His Ile Phe Asp Gly Leu Glu Thr

195 200 205

Trp Ala Thr Asp Leu Val Tyr Ile Gln Glu Gly Val Leu Lys Arg Thr

210 215 220

Glu Lys Leu Pro Glu Leu Pro Glu Leu Lys Ser Ser Pro Asn Leu Leu

225 230 235 240

Ser Val Val Glu Asn Trp Leu Arg Ser Glu Thr Pro Ile Glu Lys Lys

245 250 255

Lys Pro Ala Pro Ala Pro Ala Lys Ala Gln Lys Ser Ser Pro Phe Gly

260 265 270

Ser Ser Pro Phe Gln Ser Ser Arg His Met Ser His Tyr Arg

275 280 285

<210> 94

<211> 260

<212> PRT

<213> 芝麻(Sesamum indicum)

<220>

<221> MISC_FEATURE

<222> (1)..(260)

<223> ABC转运体

<400> 94

Met Ser Leu Asn Ile Tyr Pro Gly Ser Arg Cys Leu Leu Val Gly Ala

1 5 10 15

Asn Gly Ser Gly Lys Thr Thr Leu Leu Arg Ile Leu Ala Gly Lys His

20 25 30

Met Val Gly Gly Arg Asp Val Val Arg Val Leu Asn Cys Ser Ala Phe

35 40 45

His Asp Thr Ser Leu Val Cys Ser Gly Asp Leu Ala Tyr Leu Gly Glu

50 55 60

Ser Trp Ser Lys Thr Val Gly Ser Ala Gly Glu Leu Pro Leu Gln Gly

65 70 75 80

Asp Phe Ser Ala Glu His Met Ile Phe Gly Val Glu Gly Val Asp Pro

85 90 95

Val Arg Arg Asp Lys Leu Ile Glu Leu Leu Asp Ile Asp Leu His Trp

100 105 110

Arg Met His Lys Val Ser Asp Gly Gln Arg Arg Arg Val Gln Ile Cys

115 120 125

Met Gly Leu Leu His Pro Tyr Lys Val Leu Leu Leu Asp Glu Val Thr

130 135 140

Val Asp Leu Asp Val Val Ala Arg Met Asp Leu Leu Glu Phe Phe Lys

145 150 155 160

Glu Glu Cys Glu Gln Arg Arg Ala Thr Ile Val Tyr Ala Thr His Ile

165 170 175

Phe Asp Gly Leu Glu Thr Trp Ala Thr Asp Leu Val Tyr Val Gln Glu

180 185 190

Gly Val Leu Lys Arg Ser Asp Lys Leu Ala Asp Leu Lys Glu Leu Lys

195 200 205

Asn Asn Met Asn Leu Leu Ser Val Val Glu Ser Trp Leu Arg Thr Glu

210 215 220

Thr Lys Ile Glu Lys Lys Lys Pro Val Asn Ser Pro Ser Pro Leu Gln

225 230 235 240

Lys Ser Ser Pro Phe Asp Asn Ser Pro Phe Arg Ser Ser Arg His Met

245 250 255

Ala Tyr Tyr Arg

260

<210> 95

<211> 286

<212> PRT

<213> 马铃薯(Solanum tuberosum)

<220>

<221> MISC_FEATURE

<222> (1)..(286)

<223> ABC转运体

<400> 95

Met Glu Gly Glu Ser Ser Ser Ile Gln Val Asn Gly Met Gln Phe Ser

1 5 10 15

Tyr Asp Phe Gln Ser Pro Ile Tyr Phe Asp Phe Asn Leu Asn Ile Ala

20 25 30

Pro Arg Ser Arg Cys Leu Leu Leu Gly Ala Asn Gly Ser Gly Lys Thr

35 40 45

Thr Leu Leu Lys Ile Leu Ala Gly Lys His Met Val Gly Gly Lys Asp

50 55 60

Val Val Arg Val Leu Asn Phe Ser Ala Phe His Asp Thr His Leu Val

65 70 75 80

Cys Ser Gly Asp Leu Ala Tyr Leu Gly Glu Ser Trp Ser Lys Asn Val

85 90 95

Gly Ala Val Gly Glu Ile Pro Leu Gln Gly Asp Phe Ser Ala Glu His

100 105 110

Met Ile Phe Gly Val Glu Gly Val Asp Pro Val Arg Arg Glu Lys Leu

115 120 125

Ile Glu Leu Leu Asp Ile Asp Leu Gln Trp Arg Met His Lys Val Ser

130 135 140

Asp Gly Gln Arg Arg Arg Val Gln Ile Cys Met Gly Leu Leu His Pro

145 150 155 160

Tyr Lys Val Leu Leu Leu Asp Glu Val Thr Val Asp Leu Asp Val Val

165 170 175

Ala Arg Met Asp Leu Leu Asp Phe Phe Lys Glu Glu Cys Glu Gln Arg

180 185 190

Gly Ala Thr Ile Val Tyr Ala Thr His Ile Phe Asp Gly Leu Glu Thr

195 200 205

Trp Ala Thr Asp Leu Val Tyr Ile Gln Glu Gly Val Leu Lys Arg Thr

210 215 220

Glu Lys Leu Pro Glu Leu Pro Glu Leu Lys Ser Ser Pro Asn Leu Leu

225 230 235 240

Ser Val Val Glu Asn Trp Leu Arg Ser Glu Thr Pro Ile Glu Lys Lys

245 250 255

Lys Pro Ala Pro Ser Pro Pro Lys Ala Gln Lys Ser Ser Pro Phe Gly

260 265 270

Ser Ser Pro Phe Gln Ser Ser Arg His Met Ala Tyr Phe Arg

275 280 285

<210> 96

<211> 291

<212> PRT

<213> 亚麻荠(Camelina sativa)

<220>

<221> MISC_FEATURE

<222> (1)..(291)

<223> ABC转运体

<400> 96

Met Ala Glu Glu Lys Asp Ala Thr Ala Ser Gly Asp Asp Ala Ile Lys

1 5 10 15

Val Ser Gly Met Gln Phe Ala Tyr Glu Val Glu Asp Pro Ile Phe Phe

20 25 30

Asp Phe Asn Leu Asp Leu Pro Ala Gly Ser Arg Cys Leu Leu Val Gly

35 40 45

Ala Asn Gly Ser Gly Lys Thr Thr Leu Leu Lys Ile Leu Ala Gly Lys

50 55 60

His Met Val Gly Gly Lys Asn Val Val Gln Val Leu Ser Arg Ser Ala

65 70 75 80

Phe His Asp Thr Gln Leu Val Cys Ser Gly Asp Leu Ser Tyr Leu Gly

85 90 95

Gly Ser Trp Ser Lys Thr Val Gly Ser Ala Gly Glu Val Pro Leu Gln

100 105 110

Gly Asp Phe Ser Ala Glu His Met Ile Phe Gly Val Glu Gly Ile Asp

115 120 125

Pro Val Arg Arg Glu Lys Leu Ile Asp Leu Leu Asp Ile Asn Leu Gln

130 135 140

Trp Arg Met His Lys Val Ser Asp Gly Gln Lys Arg Arg Val Gln Ile

145 150 155 160

Cys Met Gly Leu Leu His Pro Phe Lys Val Leu Leu Leu Asp Glu Val

165 170 175

Thr Val Asp Leu Asp Val Val Ala Arg Met Asp Leu Leu Lys Phe Phe

180 185 190

Val Glu Glu Cys Asp Gln Arg Gly Ala Thr Ile Val Tyr Ala Thr His

195 200 205

Ile Phe Asp Gly Leu Glu Thr Trp Ala Thr His Leu Ala Tyr Ile Gln

210 215 220

Asp Gly Glu Leu Asn Gly Leu Ser Lys Met Ala Asp Ile Glu Glu Leu

225 230 235 240

Lys Ser Ala Pro Asn Leu Leu Ser Val Val Glu Ser Trp Leu Arg Ser

245 250 255

Glu Ile Lys Val Val Lys Lys Lys Lys Lys Pro Val Ala Ala Trp Lys

260 265 270

Pro Ser Pro Leu Asp Asn Ser Pro Phe Arg Ser Ser Arg His Met Ala

275 280 285

Tyr Tyr Arg

290

<210> 97

<211> 290

<212> PRT

<213> 拟南芥(Arabidopsis thaliana)

<220>

<221> MISC_FEATURE

<222> (1)..(290)

<223> ABC转运体

<400> 97

Met Ala Glu Lys Asp Ala Thr Ala Ser Gly Asp Asp Ala Ile Arg Val

1 5 10 15

Ser Gly Met Gln Phe Ala Tyr Glu Val Glu Asp Pro Ile Phe Phe Asp

20 25 30

Phe Asn Leu Asp Leu Pro Ala Gly Ser Arg Cys Leu Leu Val Gly Ala

35 40 45

Asn Gly Ser Gly Lys Thr Thr Leu Leu Lys Ile Leu Ala Gly Lys His

50 55 60

Met Val Gly Gly Lys Asn Val Val Gln Val Leu Ser Arg Ser Ala Phe

65 70 75 80

His Asp Thr Gln Leu Val Cys Ser Gly Asp Leu Ser Tyr Leu Gly Gly

85 90 95

Ser Trp Ser Lys Thr Val Gly Ser Ala Gly Glu Val Pro Leu Gln Gly

100 105 110

Asp Phe Ser Ala Glu His Met Ile Phe Gly Val Glu Gly Thr Asp Pro

115 120 125

Val Arg Arg Glu Lys Leu Ile Asp Leu Leu Asp Ile Asn Leu Gln Trp

130 135 140

Arg Met His Lys Val Ser Asp Gly Gln Lys Arg Arg Val Gln Ile Cys

145 150 155 160

Met Gly Leu Leu His Pro Phe Lys Val Leu Leu Leu Asp Glu Val Thr

165 170 175

Val Asp Leu Asp Val Val Ala Arg Met Asp Leu Leu Glu Phe Phe Lys

180 185 190

Glu Glu Cys Asp Gln Arg Gly Ala Thr Ile Val Tyr Ala Thr His Ile

195 200 205

Phe Asp Gly Leu Glu Thr Trp Ala Thr His Leu Ala Tyr Ile Gln Asp

210 215 220

Gly Glu Leu Asn Arg Leu Ser Lys Met Thr Asp Ile Glu Glu Leu Lys

225 230 235 240

Thr Ser Pro Asn Leu Leu Ser Val Val Glu Ser Trp Leu Arg Ser Glu

245 250 255

Ile Lys Leu Val Lys Lys Lys Lys Lys Pro Val Ala Pro Trp Lys Pro

260 265 270

Ser Pro Phe Asp Asn Ser Pro Phe Arg Ser Ser Arg His Met Ala Tyr

275 280 285

Tyr Arg

290

<210> 98

<211> 306

<212> PRT

<213> 黄瓜(Cucumis sativus)

<220>

<221> MISC_FEATURE

<222> (1)..(306)

<223> ABC转运体

<400> 98

Met Ala Asp Asn Gly Ile Ser Ile Ala Gly Asn Asp Gly Ile Glu Val

1 5 10 15

Leu Asp Glu Lys Gly Ser Thr Ser Ile Lys Val Gln Ala Met Gln Phe

20 25 30

Ser Tyr Glu Ser Asp Ser Pro Leu Phe Val Glu Phe Asn Leu Gln Val

35 40 45

Gly Ser Gly Ser Arg Cys Leu Leu Val Gly Ala Asn Gly Ser Gly Lys

50 55 60

Thr Thr Leu Leu Lys Ile Leu Ala Gly Lys His Met Val Gly Gly Arg

65 70 75 80

Asp Val Val Gln Val Leu Asn Gly Ser Ala Phe His Asp Thr Gln Leu

85 90 95

Val Cys Ser Gly Asp Leu Ala Tyr Leu Gly Gly Ser Trp Ser Arg Thr

100 105 110

Ile Ser Cys Ala Gly Glu Val Ala Leu Gln Gly Asp Phe Ser Ala Glu

115 120 125

His Met Ile Phe Gly Val Glu Gly Thr Asp Pro Glu Arg Arg Asp Arg

130 135 140

Leu Ile Asp Leu Leu Asp Ile Asp Leu Arg Trp Arg Met His Lys Val

145 150 155 160

Ser Asp Gly Gln Arg Arg Arg Val Gln Ile Cys Met Gly Leu Leu His

165 170 175

Pro Phe Lys Val Leu Leu Leu Asp Glu Val Thr Val Asp Leu Asp Val

180 185 190

Val Ala Arg Met Asp Leu Leu Asp Phe Leu Lys Glu Glu Cys Asp Gln

195 200 205

Arg Gly Ala Thr Ile Val Tyr Ala Thr His Ile Phe Asp Gly Leu Glu

210 215 220

Thr Trp Ala Thr His Leu Ala Tyr Ile Gln Asp Gly Glu Leu Arg Lys

225 230 235 240

Ser Glu Lys Leu Ser Lys Val Glu Glu Leu Lys Thr Cys Ala Asn Leu

245 250 255

Leu Ser Val Val Glu Thr Trp Leu Arg Ala Glu Thr Lys Leu Glu Lys

260 265 270

Lys Lys Lys Leu Pro Ile Gln Pro Pro Ser Asn Asn Gln Lys Ile Val

275 280 285

Ser Pro Phe Gly Ser Ser Pro Phe Met Ser Ser Arg His Met Ala Tyr

290 295 300

Tyr Arg

305

<210> 99

<211> 289

<212> PRT

<213> 山嵛菜(Eutrema salsugineum)

<220>

<221> MISC_FEATURE

<222> (1)..(289)

<223> ABC转运体

<400> 99

Met Ala Thr Thr Ser Gly Asp Asp Ala Ile Arg Val Ser Gly Met Glu

1 5 10 15

Phe Ala Tyr Glu Val Asp Asp Pro Ile Phe Phe Asp Phe Asn Leu Asp

20 25 30

Leu Pro Ala Gly Ser Arg Cys Leu Leu Val Gly Ala Asn Gly Ser Gly

35 40 45

Lys Thr Thr Leu Leu Lys Ile Leu Ala Gly Lys His Met Val Gly Gly

50 55 60

Lys Asn Val Val Gln Val Leu Ser Arg Ser Ala Phe His Asp Thr Gln

65 70 75 80

Leu Val Cys Ser Gly Asp Leu Ser Tyr Leu Gly Gly Ser Trp Ser Lys

85 90 95

Thr Val Gly Ser Ala Gly Glu Val Pro Leu Gln Gly Asp Phe Ser Ala

100 105 110

Glu His Met Ile Phe Gly Val Glu Gly Ile Asp Pro Val Arg Arg Glu

115 120 125

Lys Leu Ile Asp Leu Leu Asp Ile Asn Leu His Trp Arg Met His Lys

130 135 140

Val Ser Asp Gly Gln Lys Arg Arg Val Gln Ile Cys Met Gly Leu Leu

145 150 155 160

His Pro Phe Lys Val Leu Leu Leu Asp Glu Val Thr Val Asp Leu Asp

165 170 175

Val Val Ala Arg Met Asp Leu Leu Glu Phe Phe Lys Glu Glu Cys Asp

180 185 190

Lys Arg Gly Ala Thr Ile Val Tyr Ala Thr His Ile Phe Asp Gly Leu

195 200 205

Glu Thr Trp Ala Thr His Leu Ala Tyr Ile Gln Asn Gly Glu Leu Ile

210 215 220

Arg Ser Ser Arg Met Ala Asp Ile Asn Glu Met Lys Ile Ser Pro Asn

225 230 235 240

Leu Leu Ser Val Val Glu Ser Trp Leu Arg Ser Glu Thr Lys Leu Glu

245 250 255

Lys Lys Lys Lys Lys Lys Lys Glu Pro Ala Ala Lys Trp Lys Pro Ser

260 265 270

Pro Phe Asp Asn Ser Pro Phe Arg Ser Ser Arg His Met Ala Tyr Tyr

275 280 285

Arg

<210> 100

<211> 284

<212> PRT

<213> 野草莓(Fragaria vesca)

<220>

<221> MISC_FEATURE

<222> (1)..(284)

<223> ABC转运体

<400> 100

Met Ala Glu Pro Gln Glu Ser Glu Ala Ser Ser Gly Ile Arg Val Ser

1 5 10 15

Gly Met Gln Phe Ala Tyr Asp Ala Gln Pro Pro Leu Phe Tyr Asp Phe

20 25 30

Ser Leu Asn Val Ala Pro Gly Ser Arg Cys Leu Leu Val Gly Ala Asn

35 40 45

Gly Ser Gly Lys Thr Thr Leu Leu Lys Ile Leu Ala Gly Lys Gln Met

50 55 60

Val Gly Gly Arg Asp Val Val Arg Val Leu Asn Cys Ser Ala Phe His

65 70 75 80

Asp Thr His Leu Val Cys Ser Gly Asp Leu Ala Tyr Leu Gly Gly Ser

85 90 95

Trp Ser Lys Thr Val Gly Ser Ala Gly Glu Val Pro Leu Gln Gly Asp

100 105 110

Phe Ser Ala Glu His Met Ile Phe Gly Val Glu Gly Thr Asp Pro Val

115 120 125

Arg Arg Glu Lys Leu Ile Glu Leu Leu Asp Ile Asp Leu Lys Trp Arg

130 135 140

Met His Lys Val Ser Asp Gly Gln Arg Arg Arg Val Gln Ile Cys Met

145 150 155 160

Gly Leu Leu His Pro Phe Lys Val Leu Leu Leu Asp Glu Val Thr Val

165 170 175

Asp Leu Asp Val Val Ala Arg Met Asp Leu Leu Glu Phe Phe Lys Glu

180 185 190

Glu Cys Glu Gln Arg Gly Ala Thr Ile Val Tyr Ala Thr His Ile Phe

195 200 205

Asp Gly Leu Glu Thr Trp Ala Thr His Leu Ala Tyr Ile Gln Asp Gly

210 215 220

Glu Leu Lys Lys Ala Gln Lys Leu Leu Glu Val Glu Glu Leu Lys Asn

225 230 235 240

Ser Val Asn Leu Leu Ser Val Val Glu Ser Trp Leu Arg Ala Glu Thr

245 250 255

Lys Gln Glu Lys Lys Lys Thr Lys Asn Pro Ala Gln Thr Ser Ser Pro

260 265 270

Phe Gly Met Ser Ser Arg Gln Met Ala Tyr Tyr Arg

275 280

<210> 101

<211> 290

<212> PRT

<213> 荠菜(Capsella rubella)

<220>

<221> MISC_FEATURE

<222> (1)..(290)

<223> ABC转运体

<400> 101

Met Ala Glu Lys Asp Ala Thr Ala Ser Gly Gly Asp Ala Ile Arg Val

1 5 10 15

Ser Gly Met Gln Phe Ala Tyr Glu Val Glu Asp Pro Ile Phe Phe Asp

20 25 30

Phe Asn Leu Asp Leu Pro Val Gly Ser Arg Cys Leu Leu Val Gly Ala

35 40 45

Asn Gly Ser Gly Lys Thr Thr Leu Leu Lys Ile Leu Ala Gly Lys His

50 55 60

Met Val Gly Gly Lys Asn Val Val Gln Val Leu Ser Arg Ser Ala Phe

65 70 75 80

His Asp Thr Gln Leu Val Cys Ser Gly Asp Leu Ser Tyr Leu Gly Gly

85 90 95

Ser Trp Ser Lys Thr Val Gly Ser Ala Gly Glu Val Pro Leu Gln Gly

100 105 110

Asp Phe Ser Ala Glu His Met Ile Phe Gly Val Glu Gly Ile Asp Pro

115 120 125

Val Arg Arg Glu Lys Leu Ile Asp Leu Leu Asp Ile Asn Leu Gln Trp

130 135 140

Arg Met His Lys Val Ser Asp Gly Gln Lys Arg Arg Val Gln Ile Cys

145 150 155 160

Met Gly Leu Leu His Pro Phe Lys Val Leu Leu Leu Asp Glu Val Thr

165 170 175

Val Asp Leu Asp Val Val Ala Arg Met Asp Leu Leu Glu Phe Phe Ile

180 185 190

Glu Glu Cys Asp Gln Arg Gly Ala Thr Ile Val Tyr Ala Thr His Ile

195 200 205

Phe Asp Gly Leu Glu Thr Trp Ala Thr His Leu Ala Tyr Ile Gln Asp

210 215 220

Gly Glu Leu Asn Arg Leu Ser Lys Met Thr Asp Ile Asp Glu Leu Lys

225 230 235 240

Thr Ser Pro Asn Leu Leu Ser Val Val Glu Ser Trp Leu Arg Ser Glu

245 250 255

Ile Lys Leu Val Lys Lys Lys Lys Ile Lys Pro Val Ala Ala Trp Lys

260 265 270

Pro Ser Pro Phe Asp Asn Ser Pro Phe Arg Ser Ser Arg His Met Ala

275 280 285

Tyr Tyr

290

<210> 102

<211> 291

<212> PRT

<213> 醉蝶花(Tarenaya hassleriana)

<220>

<221> MISC_FEATURE

<222> (1)..(291)

<223> ABC转运体

<400> 102

Met Ala Glu Arg Asp Ala Ala Ala Lys Gly Gly Gly Asp Gly Asp Asp

1 5 10 15

Ala Ile Arg Val Cys Gly Met Gln Phe Ala Tyr Asp Val Gln Asp Pro

20 25 30

Leu Phe Phe Asp Phe Asn Leu Glu Leu Pro Ala Gly Ser Arg Cys Leu

35 40 45

Leu Val Gly Ala Asn Gly Ser Gly Lys Thr Thr Leu Leu Lys Ile Leu

50 55 60

Ala Gly Lys His Met Val Gly Gly Lys Asn Val Val Gln Val Leu Asn

65 70 75 80

Arg Ser Ala Phe His Asp Thr Lys Leu Val Cys Ser Gly Asp Leu Ala

85 90 95

Tyr Leu Gly Gly Ser Trp Ser Lys Thr Val Gly Ser Ala Gly Glu Val

100 105 110

Pro Leu Gln Gly Asp Phe Ser Ala Glu His Met Ile Phe Gly Val Glu

115 120 125

Gly Ile Asp Pro Ala Arg Arg Glu Lys Leu Ile Glu Leu Leu Asp Ile

130 135 140

Asp Leu Gln Trp Arg Met His Lys Val Ser Asp Gly Gln Arg Arg Arg

145 150 155 160

Val Gln Ile Cys Met Gly Leu Leu His Pro Phe Lys Val Leu Leu Leu

165 170 175

Asp Glu Val Thr Val Asp Leu Asp Val Val Ala Arg Met Asp Leu Leu

180 185 190

Glu Phe Phe Lys Glu Glu Cys Glu Gln Arg Gly Ala Thr Ile Val Tyr

195 200 205

Ala Thr His Ile Phe Asp Gly Leu Glu Thr Trp Ala Thr His Leu Ala

210 215 220

Tyr Val His Asp Gly Glu Leu Lys Arg Ser Ala Glu Thr Lys Glu Ile

225 230 235 240

Glu Glu Leu Lys Thr Ser Ala Asn Leu Leu Ser Val Val Glu Asp Trp

245 250 255

Leu Arg Ser Glu Thr Lys Asp Glu Asn Lys Lys Lys Lys Lys Pro Ala

260 265 270

Gln Asn Thr Ser Gln Ser Pro Phe Gly Met Ser Ser Arg His Met Ala

275 280 285

Tyr Tyr Arg

290

<210> 103

<211> 291

<212> PRT

<213> 天蓝遏蓝菜(Noccaea caerulescens)

<220>

<221> MISC_FEATURE

<222> (1)..(291)

<223> ABC转运体

<400> 103

Met Ala Ala Lys Asp Pro Thr Thr Thr Asn Asp Asp Ala Ile Arg Val

1 5 10 15

Ser Gly Met Glu Phe Ala Tyr Glu Val Glu Asp Pro Ile Phe Phe Asp

20 25 30

Phe Ser Leu Asp Leu Pro Ser Gly Ser Arg Cys Leu Leu Val Gly Ala

35 40 45

Asn Gly Ser Gly Lys Thr Thr Leu Leu Lys Ile Leu Ala Gly Lys His

50 55 60

Leu Val Gly Gly Arg Asn Val Val Gln Val Leu Ser Arg Ser Ala Phe

65 70 75 80

His Asp Thr Gln Leu Val Cys Ser Gly Asp Leu Ser Tyr Leu Gly Gly

85 90 95

Ser Trp Ser Lys Thr Val Gly Ser Ala Gly Glu Val Pro Leu Gln Gly

100 105 110

Asp Phe Ser Ala Glu Leu Met Ile Phe Gly Val Glu Gly Val Asp Pro

115 120 125

Val Arg Arg Glu Lys Leu Ile Asp Leu Leu Asp Ile Asn Leu His Trp

130 135 140

Arg Met His Lys Val Ser Asp Gly Gln Lys Arg Arg Val Gln Ile Cys

145 150 155 160

Met Gly Leu Leu His Pro Phe Lys Val Leu Leu Leu Asp Glu Val Thr

165 170 175

Val Asp Leu Asp Val Val Ala Arg Met Asp Leu Leu Glu Phe Phe Gln

180 185 190

Glu Glu Cys Asp Gln Arg Gly Ala Thr Ile Val Tyr Ala Thr His Ile

195 200 205

Phe Asp Gly Leu Glu Ala Trp Gly Thr His Leu Ala Tyr Ile Gln Asp

210 215 220

Gly Glu Leu Asn Arg Ser Leu Lys Met Ala Asp Ile Asp Glu Leu Lys

225 230 235 240

Thr Ser Pro Asn Leu Leu Ser Val Val Glu Ser Trp Leu Arg Ser Glu

245 250 255

Thr Lys Pro Glu Lys Lys Lys Lys Lys Lys Pro Val Ala Ala Trp Lys

260 265 270

Pro Ser Pro Phe Asp Asn Ser Pro Phe Arg Ser Ser Lys His Met Ala

275 280 285

Tyr Tyr Arg

290

Claims

2.如权利要求1所述的方法，其中，所述ABC转运体蛋白编码序列包含SEQ ID NO:1，或者编码选自SEQ ID NO:2和15-103的蛋白质。

3.一种植物，在其基因组中稳定地引入了与ABC转运体蛋白编码序列操作性连接、驱动植物中的表达的启动子，其中，所述启动子对于所述ABC转运体蛋白编码序列而言是异源的。

4.如权利要求3所述的植物，其中，所述ABC转运体蛋白编码序列包含SEQ ID NO:1，或者编码选自SEQ ID NO:2和15-103的蛋白质。

5.一种转化的种子，其是权利要求3～4中任一项所述的植物的转化的种子。

6.如权利要求3或4所述的植物，其中，所述植物是单子叶植物。

7.如权利要求3或4所述的植物，其中，所述植物是双子叶植物。

8.如权利要求1或2所述的方法，其中，所述ABC转运体蛋白编码序列自发育调控启动子表达。

9.如权利要求8所述的方法，其中，所述发育调控启动子包含SEQ ID NO:3或5。

10.如权利要求3或4所述的植物，其中，驱动植物中的表达的所述启动子是发育调控启动子。

11.如权利要求10所述的植物，其中，所述发育调控启动子包含SEQ ID NO:3或5。

12.一种DNA构建体，以操作性连接的方式包括：

a.在植物细胞中起作用的启动子，以及

b.编码ABC转运体蛋白的核酸序列。

13.如权利要求12所述的DNA构建体，其中，所述编码ABC转运体蛋白的核酸序列包含SEQ ID NO:1，或者编码选自SEQ ID NO:2和15-103的蛋白质。

14.如权利要求12或13所述的DNA构建体，其中，所述在植物细胞中起作用的启动子包含SEQ ID NO:3和5。

15.如权利要求12～14中任一项所述的DNA构建体，其中，所述启动子对于所述编码ABC转运体蛋白的核酸序列而言是异源的。