CO5290304A1 - Transformacion del sorgo mediante agrobacterium - Google Patents

Transformacion del sorgo mediante agrobacterium

Info

Publication number
CO5290304A1
CO5290304A1 CO98023424A CO98023424A CO5290304A1 CO 5290304 A1 CO5290304 A1 CO 5290304A1 CO 98023424 A CO98023424 A CO 98023424A CO 98023424 A CO98023424 A CO 98023424A CO 5290304 A1 CO5290304 A1 CO 5290304A1
Authority
CO
Colombia
Prior art keywords
agrobacterium
nucleotide sequence
tissue
transformation
sorghum
Prior art date
Application number
CO98023424A
Other languages
English (en)
Inventor
Dorothy A Pierce
Laura A Tagliani
Zuo-Yu Zhao
Original Assignee
Pierce Dorothy
Laura A Tagliani
Zuo-Yu Zhao
Cai Tishu
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Pierce Dorothy, Laura A Tagliani, Zuo-Yu Zhao, Cai Tishu filed Critical Pierce Dorothy
Publication of CO5290304A1 publication Critical patent/CO5290304A1/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8202—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by biological means, e.g. cell mediated or natural vector
    • C12N15/8205—Agrobacterium mediated transformation

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Cell Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

Un método para la transformación del sorgo con una secuencia de nucleótidos de interés, caracterizado por comprender las etapas de:Contactar un tejido de una planta de sorgo con Agrobacterium que incluye un vector que comprende dicha secuencia de nucleótidos, donde dicha secuencia de nucleótidos se caracteriza por comprender al menos una cassette de expresión con un gen que confiere resistencia a un agente de selección;Cultivar en forma conjunta el tejido con el Agrobacterium; Cultivar el tejido en un medio que comprende un antibiótico capaz de inhibir el crecimiento del Agrobacterium y el mencionado agente de selección;Regenerar las plantas de sorgo transformadas.<EMI FILE="98023424_1" ID="1" IMF=JPEG >
CO98023424A 1997-04-30 1998-04-29 Transformacion del sorgo mediante agrobacterium CO5290304A1 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US4512197P 1997-04-30 1997-04-30
US09/056,418 US6369298B1 (en) 1997-04-30 1998-04-07 Agrobacterium mediated transformation of sorghum

Publications (1)

Publication Number Publication Date
CO5290304A1 true CO5290304A1 (es) 2003-06-27

Family

ID=26722402

Family Applications (1)

Application Number Title Priority Date Filing Date
CO98023424A CO5290304A1 (es) 1997-04-30 1998-04-29 Transformacion del sorgo mediante agrobacterium

Country Status (5)

Country Link
US (2) US6369298B1 (es)
AR (1) AR015805A1 (es)
AU (1) AU743706B2 (es)
CO (1) CO5290304A1 (es)
WO (1) WO1998049332A1 (es)

Families Citing this family (61)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2267014A1 (en) * 1999-03-26 2000-09-26 Brenda Rojas Monocot transformation using acrobacterium
US6875907B2 (en) 2000-09-13 2005-04-05 Pioneer Hi-Bred International, Inc. Antimicrobial peptides and methods of use
ATE509111T1 (de) 2002-03-20 2011-05-15 Simplot Co J R Verfeinerte pflanzentransformation
US20040133938A1 (en) * 2002-11-19 2004-07-08 Yinghui Dan Use of lipoic acid in plant culture media
EP2078753A3 (en) 2002-12-26 2010-12-15 Syngenta Participations AG Cell proliferation-related polypeptides and uses therefor
US7119255B2 (en) 2004-03-08 2006-10-10 Syngenta Participations, Ag Promoter from maize prolamin seed storage protein and uses thereof
CA2592894C (en) 2004-12-28 2013-08-27 Pioneer Hi-Bred International, Inc. Improved grain quality through altered expression of gamma-zein protein
US7611898B2 (en) 2005-05-09 2009-11-03 The Samuel Roberts Noble Foundation Agrobacterium transformation of stolons
ES2641088T3 (es) 2006-12-07 2017-11-07 Kansas State University Research Foundation Sorgo resistente a herbicidas de acetolactato sintetasa
ES2634795T3 (es) 2007-01-12 2017-09-29 Kansas State University Research Foundation Sorgo resistente a herbicidas inhibidores de acetil-CoA-carboxilasa
BRPI0816750A2 (pt) 2007-06-29 2015-09-29 Pioneer Hi Bred Int métodos para alterar o genoma de uma célula de planta monocotiledônea e para modificar uma sequência alvo genômica endógena específica e planta de milho
EP2036983A1 (de) * 2007-09-12 2009-03-18 Bayer CropScience AG Pflanzen, die erhöhte Mengen an Glucosaminglycanen synthetisieren
EP2222859A2 (en) 2007-12-21 2010-09-01 Keygene N.V. Trichome specific promoters
US8143484B2 (en) * 2008-01-23 2012-03-27 Pioneer Hi-Bred International, Inc. Method for regulating agrobacterium-mediated transformation
WO2010071418A1 (en) 2008-12-19 2010-06-24 Keygene N.V. Recql5 in homologous recombination
US8404930B2 (en) * 2009-01-26 2013-03-26 Pioneer Hi-Bred International, Inc. Methods for improving monocot transformation
US8373025B2 (en) 2009-02-09 2013-02-12 Chromatin Germplasm, Llc Herbicide resistant sorghum
EA025000B1 (ru) 2009-06-08 2016-11-30 Нунхемс Б.В. Засухоустойчивые растения
AU2010339404B2 (en) 2009-12-30 2016-01-28 Pioneer Hi-Bred International, Inc. Methods and compositions for the introduction and regulated expression of genes in plants
EA030460B1 (ru) 2010-05-28 2018-08-31 Нунхемс Б.Ф. Растения с увеличенным размером плода
EP2597943B1 (en) * 2010-07-29 2018-02-07 Dow AgroSciences LLC Strains of agrobacterium modified to increase plant transformation frequency
US9556448B2 (en) 2011-04-11 2017-01-31 Targeted Growth, Inc. Identification and the use of KRP mutants in plants
US20140206850A1 (en) 2011-04-29 2014-07-24 Keygene N.V. Glyphosate resistance enhancement
WO2012159891A1 (en) 2011-05-20 2012-11-29 Syngenta Participations Ag Endosperm-specific plant promoters and uses therefor
US9365862B1 (en) 2011-05-26 2016-06-14 Nutech Ventures Herbicide resistant sorghum mutants
JP6215196B2 (ja) 2011-05-31 2017-10-18 キージーン・エン・フェー 有害生物耐性植物
WO2013048254A1 (en) 2011-09-30 2013-04-04 Keygene N.V. Heat tolerance microrna
WO2013048255A2 (en) 2011-09-30 2013-04-04 Keygene N.V. Heat tolerance microrna
US20140123339A1 (en) 2012-10-31 2014-05-01 Pioneer Hi Bred International Inc Transformed Plants Having Increased Beta-Carotene Levels, Increased Half-Life and Bioavailability and Methods of Producing Such
LT3062606T (lt) 2013-10-29 2019-07-10 Biotech Institute, Llc Specializuotų kanapių selekcija, gamyba, perdirbimas ir panaudojimas
JP2018027020A (ja) * 2014-12-24 2018-02-22 日本たばこ産業株式会社 アグロバクテリウム菌を用いた、ソルガム属植物への遺伝子導入方法およびソルガム属植物の形質転換植物の作成方法
EP3344774A1 (en) 2015-09-04 2018-07-11 Keygene N.V. Diplospory gene
WO2018087301A1 (en) 2016-11-10 2018-05-17 Keygene N.V. Methods for improving drought resistance in plants - kgdr06, kgdr26, kgdr25, kgdr42 and kgdr37
WO2018101824A1 (en) 2016-11-30 2018-06-07 Universiteit Van Amsterdam Plants comprising pathogen effector constructs
WO2018146322A1 (en) 2017-02-13 2018-08-16 Keygene N.V. Method for altering ripening characteristics of fruit
WO2018146323A1 (en) 2017-02-13 2018-08-16 Keygene N.V. Method for altering ripening characteristics of fruit
MX2020010180A (es) 2018-03-30 2020-10-28 Keygene Nv Loci de rasgos cuantitativos (qtls) para la resistencia al mildiu polvoriento en el melon.
CN108504672B (zh) * 2018-03-30 2022-09-27 南京农业大学 青枯菌n477胞外蛋白phd及其编码基因和应用
WO2019193143A1 (en) 2018-04-05 2019-10-10 Keygene N.V. Improved shoot regeneration by overexpression of chk genes
NL2020823B1 (en) 2018-04-25 2019-11-05 Univ Delft Tech NAD+ dependent 7ß-hydroxysteroid dehydrogenase
EP3787395A1 (en) 2018-05-01 2021-03-10 Keygene N.V. Genes for hormone-free plant regeneration
JP7396770B2 (ja) 2018-07-12 2023-12-12 キージーン ナムローゼ フェンノートシャップ 植物細胞におけるゲノム編集のためのｖ型ｃｒｉｓｐｒ／ヌクレアーゼシステム
WO2020089448A1 (en) 2018-11-01 2020-05-07 Keygene N.V. Dual guide rna for crispr/cas genome editing in plants cells
WO2020169830A1 (en) 2019-02-21 2020-08-27 Keygene N.V. Genotyping of polyploids
NL2022905B1 (en) 2019-04-09 2020-10-20 Univ Delft Tech Yeast with engineered Molybdenum co-factor biosynthesis
NL2023169B1 (en) 2019-05-21 2020-12-01 Univ Delft Tech Biotin prototrophy
CA3138988A1 (en) 2019-05-29 2020-12-03 Keygene N.V. Gene for parthenogenesis
WO2021122982A1 (en) 2019-12-19 2021-06-24 Keygene N.V. Germacrene a synthase mutants
KR20230088741A (ko) 2020-10-13 2023-06-20 키진 엔.브이. 단위생식 유전자의 변형된 프로모터
AU2022220203A1 (en) 2021-02-11 2023-08-24 Koninklijke Nederlandse Akademie Van Wetenschappen Curing disease by transcription regulatory gene editing
WO2022219175A1 (en) 2021-04-15 2022-10-20 Keygene N.V. Mobile endonucleases for heritable mutations
BR112023021051A2 (pt) 2021-04-15 2023-12-26 Keygene Nv Plantas recalcitrantes de corregeneração
EP4186917A1 (en) 2021-11-29 2023-05-31 Keygene N.V. Tobamovirus resistant plants
WO2023111225A1 (en) 2021-12-17 2023-06-22 Keygene N.V. Double decapitation of plants
WO2024008763A2 (en) 2022-07-05 2024-01-11 Keygene N.V. Orobanche resistant plants
WO2024023067A1 (en) 2022-07-25 2024-02-01 Koninklijke Nederlandse Akademie Van Wetenschappen Curing disease by transcription regulatory gene editing
WO2024047210A1 (en) 2022-09-01 2024-03-07 Keygene N.V. Hydrogel-carriers for induction of plant morphogenesis
US20240093220A1 (en) 2022-09-09 2024-03-21 Friedrich Alexander Universität Erlangen-Nürnberg Plant regulatory elements and uses thereof
WO2024084025A1 (en) 2022-10-21 2024-04-25 Keygene N.V. Rna transfection in plant cells with modified rna
WO2024133851A1 (en) 2022-12-22 2024-06-27 Keygene N.V. Regeneration by protoplast callus grafting
WO2024146898A1 (en) 2023-01-03 2024-07-11 Keygene N.V. Nal1 in rice

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5773691A (en) * 1992-03-19 1998-06-30 E. I. Du Pont De Nemours And Company Chimeric genes and methods for increasing the lysine and threonine content of the seeds of plants
WO1994000977A1 (en) 1992-07-07 1994-01-20 Japan Tobacco Inc. Method of transforming monocotyledon
AU4469793A (en) 1992-08-19 1994-02-24 Monsanto Company Method for transforming monocotyledonous plants
PT672752E (pt) 1993-09-03 2004-10-29 Japan Tobacco Inc Processo de transformacao de uma monocotiledonea com a utilizacao de um escutelode um embriao imaturo
US5731179A (en) 1993-12-08 1998-03-24 Japan Tobacco Inc. Method for introducing two T-DNAS into plants and vectors therefor
US5631152A (en) 1994-10-26 1997-05-20 Monsanto Company Rapid and efficient regeneration of transgenic plants

Also Published As

Publication number Publication date
WO1998049332A1 (en) 1998-11-05
US6369298B1 (en) 2002-04-09
AU7134298A (en) 1998-11-24
AR015805A1 (es) 2001-05-30
AU743706B2 (en) 2002-01-31
US20020138879A1 (en) 2002-09-26
WO1998049332A8 (en) 1999-04-01

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