BRPI0413830A - método para a reprodução de fungos que produzem lipìdio - Google Patents

método para a reprodução de fungos que produzem lipìdio

Info

Publication number
BRPI0413830A
BRPI0413830A BRPI0413830-9A BRPI0413830A BRPI0413830A BR PI0413830 A BRPI0413830 A BR PI0413830A BR PI0413830 A BRPI0413830 A BR PI0413830A BR PI0413830 A BRPI0413830 A BR PI0413830A
Authority
BR
Brazil
Prior art keywords
lipid
reproduction
auxotrophy
marker
host
Prior art date
Application number
BRPI0413830-9A
Other languages
English (en)
Inventor
Misa Ochiai
Hiroshi Kawashima
Sakayu Shimizui
Original Assignee
Suntory Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Suntory Ltd filed Critical Suntory Ltd
Publication of BRPI0413830A publication Critical patent/BRPI0413830A/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C12N1/205—Bacterial isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/02—Preparation of hybrid cells by fusion of two or more cells, e.g. protoplast fusion
    • C12N15/03—Bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8206—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by physical or chemical, i.e. non-biological, means, e.g. electroporation, PEG mediated
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6436—Fatty acid esters
    • C12P7/6445—Glycerides
    • C12P7/6463—Glycerides obtained from glyceride producing microorganisms, e.g. single cell oil

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Cell Biology (AREA)
  • Mycology (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Farming Of Fish And Shellfish (AREA)

Abstract

"MéTODO PARA A REPRODUçãO DE FUNGOS QUE PRODUZEM LIPìDIO". Se pretende proporcionar um método de reprodução pelo qual um fungo que produz lipídio pertencendo ao gênero Mortierella pode ser efetiva e eficientemente reproduzido sem recorrer a antibióticos. A transformação é realizada com o uso de um auxótrofo (por exemplo, auxótrofo de uracila) de um fungo pertencendo à Mortierella como um hospedeiro. Mais especialmente falando, um gene que compensa a auxotrofia, o qual é empregado como um marcador, é transferido para o hospedeiro (a etapa de transferência de gene) . Em seguida, um transformante é selecionado através de uso de recuperação da auxotrofia como o marcador (a etapa de seleção). O fungo que produz lipídio pode ser M. aplina, por exemplo.
BRPI0413830-9A 2003-08-22 2004-08-20 método para a reprodução de fungos que produzem lipìdio BRPI0413830A (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2003299345 2003-08-22
PCT/JP2004/012021 WO2005019437A1 (ja) 2003-08-22 2004-08-20 脂質生産菌の育種方法

Publications (1)

Publication Number Publication Date
BRPI0413830A true BRPI0413830A (pt) 2006-10-24

Family

ID=34213753

Family Applications (1)

Application Number Title Priority Date Filing Date
BRPI0413830-9A BRPI0413830A (pt) 2003-08-22 2004-08-20 método para a reprodução de fungos que produzem lipìdio

Country Status (14)

Country Link
US (1) US20070072275A1 (pt)
EP (1) EP1666584B1 (pt)
JP (1) JP4537319B2 (pt)
KR (1) KR101187081B1 (pt)
CN (1) CN100381558C (pt)
AT (1) ATE493489T1 (pt)
AU (1) AU2004267294B2 (pt)
BR (1) BRPI0413830A (pt)
CA (1) CA2536606C (pt)
DE (1) DE602004030807D1 (pt)
DK (1) DK1666584T3 (pt)
MX (1) MXPA06002074A (pt)
RU (1) RU2340665C2 (pt)
WO (1) WO2005019437A1 (pt)

Families Citing this family (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2169055B1 (en) * 2007-05-25 2015-04-01 Suntory Holdings Limited Novel lysophosphatidate acyltransferase gene
RU2485179C2 (ru) 2007-06-18 2013-06-20 Сантори Холдингз Лимитед Гомологи глицерин-3-фосфатацилтрансферазы (гфат) и их использование
DK2172548T3 (en) 2007-07-11 2017-01-30 Suntory Holdings Ltd Phosphatidic acid phosphatase homologs and applications thereof
AU2008280929B2 (en) * 2007-07-23 2013-09-19 Suntory Holdings Limited Fatty acid compositions having novel fatty acid rate
BRPI0818389A2 (pt) 2007-10-26 2014-10-14 Suntory Holdings Ltd Genes atp:citrato liase
AU2010225738B2 (en) 2009-03-18 2015-01-29 Suntory Holdings Limited Novel acetyl-CoA carboxylase
WO2010110375A1 (ja) 2009-03-26 2010-09-30 サントリーホールディングス株式会社 新規なリゾリン脂質アシル基転移酵素
JP5475011B2 (ja) 2009-12-21 2014-04-16 サントリーホールディングス株式会社 ジアシルグリセロールアシル基転移酵素遺伝子及びその用途
CA2787832C (en) 2010-02-01 2016-01-05 Suntory Holdings Limited Polynucleotide encoding acyl-coa synthetase homolog and use thereof
JP5890687B2 (ja) 2010-02-03 2016-03-22 サントリーホールディングス株式会社 グリセロール−３−リン酸アシル基転移酵素ホモログとその利用
WO2012125959A2 (en) 2011-03-17 2012-09-20 Solazyme, Inc. Pyrolysis oil and other combustible compositions from microbial biomass
JP6392208B2 (ja) 2013-03-27 2018-09-19 サントリーホールディングス株式会社 モルティエレラ属微生物内で高発現活性を示すプロモーター
CN103468581B (zh) * 2013-08-09 2015-09-16 江南大学 一种通过同源重组敲除ura5基因的高山被孢霉尿嘧啶营养缺陷型及其构建方法
CN103642698B (zh) * 2013-11-18 2015-11-04 嘉必优生物工程（武汉）有限公司 高山被孢霉突变株及其应用
CN105802952B (zh) * 2016-03-29 2018-11-20 上海市农业科学院 一种利用尿嘧啶营养缺陷型进行香菇菌种保护的方法
CN110747133B (zh) * 2019-11-18 2021-03-02 河南农业大学 一株头孢被孢霉、包括头孢被孢霉的菌剂及其制备方法和应用

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5204250A (en) * 1986-03-31 1993-04-20 Suntory Limited Process for production of arachidonic acid
JPH07143894A (ja) * 1993-11-24 1995-06-06 Mitsubishi Chem Corp タンパク質の生産方法
KR100222639B1 (ko) * 1997-07-29 1999-10-01 손경식 신규한 대장균 변이주 cj 181 및 그를 이용한 l-트립토판 제조방법
DE19814853A1 (de) * 1998-04-02 1999-10-21 Consortium Elektrochem Ind Expressionssystem zur Produktion von Proteinen
EP1080210A2 (en) * 1998-05-27 2001-03-07 Novo Nordisk Biotech, Inc. Methods for producing a polypeptide by modifying the copy number of a gene
JP2000069987A (ja) * 1998-08-28 2000-03-07 Suntory Ltd アラキドン酸含有脂質並びにジホモ−γ−リノレン酸含有脂質の製造方法
JP2000125859A (ja) * 1998-10-26 2000-05-09 Meito Sangyo Co Ltd 新規な選択マーカー遺伝子ならびにリゾムコール・プシルスの形質転換系
US6689601B2 (en) * 2000-09-01 2004-02-10 E. I. Du Pont De Nemours And Company High growth methanotropic bacterial strain
US7214491B2 (en) * 2003-05-07 2007-05-08 E. I. Du Pont De Nemours And Company Δ-12 desaturase gene suitable for altering levels of polyunsaturated fatty acids in oleaginous yeasts
CA2586309C (en) * 2004-11-04 2014-05-27 Monsanto Technology Llc High pufa oil compositions

Also Published As

Publication number Publication date
RU2340665C2 (ru) 2008-12-10
RU2006108790A (ru) 2007-10-10
CA2536606A1 (en) 2005-03-03
JPWO2005019437A1 (ja) 2007-11-01
JP4537319B2 (ja) 2010-09-01
CA2536606C (en) 2013-04-30
US20070072275A1 (en) 2007-03-29
ATE493489T1 (de) 2011-01-15
EP1666584A1 (en) 2006-06-07
CN1839199A (zh) 2006-09-27
DE602004030807D1 (de) 2011-02-10
EP1666584B1 (en) 2010-12-29
AU2004267294B2 (en) 2008-08-28
MXPA06002074A (es) 2006-05-25
AU2004267294A1 (en) 2005-03-03
EP1666584A4 (en) 2007-01-10
WO2005019437A1 (ja) 2005-03-03
KR101187081B1 (ko) 2012-09-27
KR20070031827A (ko) 2007-03-20
CN100381558C (zh) 2008-04-16
DK1666584T3 (da) 2011-02-28

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B06F Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette]
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