DE602004030807D1 - Verfahren zur züchtung eines lipidproduzierenden pilzes - Google Patents

Verfahren zur züchtung eines lipidproduzierenden pilzes

Info

Publication number
DE602004030807D1
DE602004030807D1 DE602004030807T DE602004030807T DE602004030807D1 DE 602004030807 D1 DE602004030807 D1 DE 602004030807D1 DE 602004030807 T DE602004030807 T DE 602004030807T DE 602004030807 T DE602004030807 T DE 602004030807T DE 602004030807 D1 DE602004030807 D1 DE 602004030807D1
Authority
DE
Germany
Prior art keywords
lipid
breeding
auxotrophy
marker
host
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
DE602004030807T
Other languages
English (en)
Inventor
Misa Ochiai
Hiroshi Kawashima
Sakayu Shimizu
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Suntory Holdings Ltd
Original Assignee
Suntory Holdings Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Suntory Holdings Ltd filed Critical Suntory Holdings Ltd
Publication of DE602004030807D1 publication Critical patent/DE602004030807D1/de
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C12N1/205—Bacterial isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/02—Preparation of hybrid cells by fusion of two or more cells, e.g. protoplast fusion
    • C12N15/03—Bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8206—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by physical or chemical, i.e. non-biological, means, e.g. electroporation, PEG mediated
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6436—Fatty acid esters
    • C12P7/6445—Glycerides
    • C12P7/6463—Glycerides obtained from glyceride producing microorganisms, e.g. single cell oil

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Cell Biology (AREA)
  • Mycology (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Farming Of Fish And Shellfish (AREA)
DE602004030807T 2003-08-22 2004-08-20 Verfahren zur züchtung eines lipidproduzierenden pilzes Active DE602004030807D1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2003299345 2003-08-22
PCT/JP2004/012021 WO2005019437A1 (ja) 2003-08-22 2004-08-20 脂質生産菌の育種方法

Publications (1)

Publication Number Publication Date
DE602004030807D1 true DE602004030807D1 (de) 2011-02-10

Family

ID=34213753

Family Applications (1)

Application Number Title Priority Date Filing Date
DE602004030807T Active DE602004030807D1 (de) 2003-08-22 2004-08-20 Verfahren zur züchtung eines lipidproduzierenden pilzes

Country Status (14)

Country Link
US (1) US20070072275A1 (de)
EP (1) EP1666584B1 (de)
JP (1) JP4537319B2 (de)
KR (1) KR101187081B1 (de)
CN (1) CN100381558C (de)
AT (1) ATE493489T1 (de)
AU (1) AU2004267294B2 (de)
BR (1) BRPI0413830A (de)
CA (1) CA2536606C (de)
DE (1) DE602004030807D1 (de)
DK (1) DK1666584T3 (de)
MX (1) MXPA06002074A (de)
RU (1) RU2340665C2 (de)
WO (1) WO2005019437A1 (de)

Families Citing this family (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK2169055T3 (da) * 2007-05-25 2015-06-22 Suntory Holdings Ltd Nyt lysophosphatidat-acyltransferasegen.
US8247209B2 (en) 2007-06-18 2012-08-21 Suntory Holdings Limited Glycerol-3-phosphate acyltransferase (GPAT) homologs and use thereof
EP2172548B1 (de) 2007-07-11 2016-12-21 Suntory Holdings Limited Phosphatidsäure-phosphatase-homologe und ihre verwendung
EP2182071B1 (de) * 2007-07-23 2016-12-14 Suntory Holdings Limited Verfahren zur Herstellung einer Fettsäurezusammensetzung
CN103290035B (zh) 2007-10-26 2015-01-21 三得利控股株式会社 新型atp:柠檬酸裂解酶基因
BRPI1013611A8 (pt) 2009-03-18 2015-12-15 Suntory Holdings Ltd acetil-coa carboxilase
CA2755387C (en) 2009-03-26 2018-06-26 Suntory Holdings Limited Mortierella alpina lysophospholipid acyltransferase
KR101438981B1 (ko) 2009-12-21 2014-09-11 산토리 홀딩스 가부시키가이샤 디아실글리세롤아실기 전이 효소 유전자 및 그 용도
AU2011211291B2 (en) 2010-02-01 2014-05-15 Suntory Holdings Limited Polynucleotide encoding acyl-CoA synthetase homolog and use thereof
BR112012019566A2 (pt) 2010-02-03 2015-09-15 Suntory Holdings Ltd homólogo de glicerol 3-fosfato aciltransferase e uso do mesmo
US8951308B2 (en) 2011-03-17 2015-02-10 Solazyme, Inc. Pyrolysis oil and other combustible compositions from microbial biomass
AU2014244851A1 (en) 2013-03-27 2015-10-22 Suntory Holdings Limited Promoter exhibiting high expression activity in Mortierella microorganisms
CN103468581B (zh) * 2013-08-09 2015-09-16 江南大学 一种通过同源重组敲除ura5基因的高山被孢霉尿嘧啶营养缺陷型及其构建方法
CN103642698B (zh) * 2013-11-18 2015-11-04 嘉必优生物工程（武汉）有限公司 高山被孢霉突变株及其应用
CN105802952B (zh) * 2016-03-29 2018-11-20 上海市农业科学院 一种利用尿嘧啶营养缺陷型进行香菇菌种保护的方法
CN110747133B (zh) * 2019-11-18 2021-03-02 河南农业大学 一株头孢被孢霉、包括头孢被孢霉的菌剂及其制备方法和应用

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5204250A (en) * 1986-03-31 1993-04-20 Suntory Limited Process for production of arachidonic acid
JPH07143894A (ja) * 1993-11-24 1995-06-06 Mitsubishi Chem Corp タンパク質の生産方法
KR100222639B1 (ko) * 1997-07-29 1999-10-01 손경식 신규한 대장균 변이주 cj 181 및 그를 이용한 l-트립토판 제조방법
DE19814853A1 (de) * 1998-04-02 1999-10-21 Consortium Elektrochem Ind Expressionssystem zur Produktion von Proteinen
AU4213999A (en) * 1998-05-27 1999-12-13 Novo Nordisk Biotech, Inc. Methods for producing a polypeptide by modifying the copy number of a gene
JP2000069987A (ja) * 1998-08-28 2000-03-07 Suntory Ltd アラキドン酸含有脂質並びにジホモ−γ−リノレン酸含有脂質の製造方法
JP2000125859A (ja) * 1998-10-26 2000-05-09 Meito Sangyo Co Ltd 新規な選択マーカー遺伝子ならびにリゾムコール・プシルスの形質転換系
US6689601B2 (en) * 2000-09-01 2004-02-10 E. I. Du Pont De Nemours And Company High growth methanotropic bacterial strain
US7214491B2 (en) * 2003-05-07 2007-05-08 E. I. Du Pont De Nemours And Company Δ-12 desaturase gene suitable for altering levels of polyunsaturated fatty acids in oleaginous yeasts
BRPI0517095B1 (pt) * 2004-11-04 2018-05-29 Monsanto Technology Llc Processo para preparação de uma composição de óleo

Also Published As

Publication number Publication date
JP4537319B2 (ja) 2010-09-01
CA2536606A1 (en) 2005-03-03
MXPA06002074A (es) 2006-05-25
CN1839199A (zh) 2006-09-27
RU2006108790A (ru) 2007-10-10
KR101187081B1 (ko) 2012-09-27
ATE493489T1 (de) 2011-01-15
EP1666584A4 (de) 2007-01-10
WO2005019437A1 (ja) 2005-03-03
AU2004267294A1 (en) 2005-03-03
US20070072275A1 (en) 2007-03-29
DK1666584T3 (da) 2011-02-28
EP1666584B1 (de) 2010-12-29
BRPI0413830A (pt) 2006-10-24
RU2340665C2 (ru) 2008-12-10
AU2004267294B2 (en) 2008-08-28
CA2536606C (en) 2013-04-30
JPWO2005019437A1 (ja) 2007-11-01
CN100381558C (zh) 2008-04-16
KR20070031827A (ko) 2007-03-20
EP1666584A1 (de) 2006-06-07

Similar Documents

Publication Publication Date Title
DE602004030807D1 (de) Verfahren zur züchtung eines lipidproduzierenden pilzes
ATE550430T1 (de) Aus bakterien der gattung corynebacterium stammende promoternukleinsäure , den promoter umfassende expressionskassette , den vektor umfassende wirtszelle sowie verfahren zur expression eines gens unter verwendung der zelle .
EP4306116A3 (de) Verfahren und zusammensetzungen zur bearbeitung von rnas
DK1748068T3 (da) Fremgangsmåde til dyrkning af en lipidproducerende stamme og udnyttelse deraf
WO2003102214A3 (en) Method for efficient rna interference in mammalian cells
DE602004012881D1 (en) Cst (compartmentalised self tagging)
ATE370224T1 (de) Verfahren zur herstellung von l-cystein unter verwendung eines zur gattung escherichia gehörenden bakteriums
TW200643037A (en) Process for macroporous acrylic resins
EP1841871A4 (de) Neuer l-lysin-induzierbarer promotor
BR0309766A (pt) Promotores usp semente especìficos para expressar genes em vegetais
WO2005063991A3 (en) Live genetically attenuated malaria vaccine
AU2002314098A1 (en) Process for the preparation of d-pantothenic acid and/or salts thereof
WO2006133257A3 (en) Use of cis-epoxyeicosatrienoic acids and inhibitors of soluble epoxide hydrolase to reduce cardiomyopathy
ATE451452T1 (de) Verfahren zur enzymatischen reduktion von alkinderivaten
ATE470715T1 (de) Oxidoreduktase aus metschnikowia zobellii
DK1766036T3 (da) Fremgangsmåde til fjernelse af selekterbare markørgensekvenser
WO2008060602A3 (en) Polymorphisms in the urocortin 3 gene and their associations with marbling and subcutaneous fat depth in beef cattle
TW200602488A (en) Method of screening siRNA
TW200737767A (en) Method of data transferring for bluetooth mouse
Lino et al. Proeftuin: a Dutch model for participative co-creation in cultural urban development
Martin et al. Retrobiosyntethic” Design for the Microbial Production of an Organic Compound
WO2003060656A3 (en) Simulation of gene expression control using connectrons.
AU2002335257A1 (en) Chimeric mouse with regulated bradeion gene expression
AU2003900786A0 (en) Methods of regulating gene expression
AU2002953553A0 (en) Method of generating queries by manipulating existing query data