AU4213999A - Methods for producing a polypeptide by modifying the copy number of a gene - Google Patents

Methods for producing a polypeptide by modifying the copy number of a gene

Info

Publication number
AU4213999A
AU4213999A AU42139/99A AU4213999A AU4213999A AU 4213999 A AU4213999 A AU 4213999A AU 42139/99 A AU42139/99 A AU 42139/99A AU 4213999 A AU4213999 A AU 4213999A AU 4213999 A AU4213999 A AU 4213999A
Authority
AU
Australia
Prior art keywords
polypeptide
gene
modifying
producing
methods
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
AU42139/99A
Inventor
Debbie S. Yaver
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Novozymes Inc
Original Assignee
Novo Nordisk Biotech Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novo Nordisk Biotech Inc filed Critical Novo Nordisk Biotech Inc
Publication of AU4213999A publication Critical patent/AU4213999A/en
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
    • C12N9/2437—Cellulases (3.2.1.4; 3.2.1.74; 3.2.1.91; 3.2.1.150)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C12N15/69—Increasing the copy number of the vector
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18—Carboxylic ester hydrolases (3.1.1)
    • C12N9/20—Triglyceride splitting, e.g. by means of lipase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01004—Cellulase (3.2.1.4), i.e. endo-1,4-beta-glucanase

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Mycology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
AU42139/99A 1998-05-27 1999-05-27 Methods for producing a polypeptide by modifying the copy number of a gene Abandoned AU4213999A (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US8550598A 1998-05-27 1998-05-27
US09085505 1998-05-27
PCT/US1999/011816 WO1999061651A2 (en) 1998-05-27 1999-05-27 Methods for producing a polypeptide by modifying the copy number of a gene

Publications (1)

Publication Number Publication Date
AU4213999A true AU4213999A (en) 1999-12-13

Family

ID=22192064

Family Applications (1)

Application Number Title Priority Date Filing Date
AU42139/99A Abandoned AU4213999A (en) 1998-05-27 1999-05-27 Methods for producing a polypeptide by modifying the copy number of a gene

Country Status (5)

Country Link
EP (1) EP1080210A2 (en)
JP (1) JP2002516116A (en)
CN (1) CN1307642A (en)
AU (1) AU4213999A (en)
WO (1) WO1999061651A2 (en)

Families Citing this family (34)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2002535990A (en) * 1999-02-02 2002-10-29 ノボザイムス バイオテック，インコーポレイティド Methods for producing polypeptides in fungal cells
CN1312260A (en) * 2000-03-07 2001-09-12 上海博德基因开发有限公司 Human proteinase regulating protein 10 as one new polypeptide and polynucleotides encoding this polypeptide
US20020164613A1 (en) * 2000-11-06 2002-11-07 Villarete Lorelie H. Method of determining gene copy number
ATE460488T1 (en) 2002-04-22 2010-03-15 Novozymes Inc METHOD FOR PRODUCING VARIANTS OF A DNA SEQUENCE IN FILAMENTOUS FUNGI
DK1622921T3 (en) 2003-05-02 2010-09-13 Novozymes Inc Variants of beta-glucosidases
WO2005019437A1 (en) * 2003-08-22 2005-03-03 Suntory Limited Method of breeding lipid-producing fungus
ES2538824T3 (en) 2003-08-25 2015-06-24 Novozymes, Inc. Variants of glycoside hydrolases
ES2437198T3 (en) 2003-10-28 2014-01-09 Novozymes Inc. Polypeptides with beta-glucosidase activity and isolated polynucleotides encoding the polypeptides
WO2005067531A2 (en) 2004-01-16 2005-07-28 Novozymes Inc. Methods for degrading lignocellulosic materials
DK2305702T3 (en) 2004-01-30 2014-06-16 Novozymes Inc Polypeptides with cellulolytic enhancing activity and polynucleotides encoding them
CN1993475A (en) 2004-05-27 2007-07-04 诺维信股份有限公司 Methods for transforming and expression screening of filamentous fungal cells with a DNA library
CN101253263B (en) 2005-04-27 2014-07-02 诺维信股份有限公司 Polypeptides having endoglucanase activity and polynucleotides encoding same
EP2385110A3 (en) 2005-09-30 2011-11-16 Novozymes, Inc. Methods for enhancing the degradation or conversion of cellulosic material
EP2374877A3 (en) 2006-03-30 2012-11-07 Novozymes A/S Polypeptides having endoglucanase activity and polynucleotides encoding same
FR2927089B1 (en) * 2008-02-05 2011-03-25 Inst Nat De La Rech Agronomique Inra METHOD OF TARGETED INTEGRATION OF MULTICOPIES OF A GENE OF INTEREST IN A YARROWIA STRAIN
EP2358878B1 (en) 2008-11-20 2014-10-15 Novozymes Inc. Polypeptides having amylolytic enhancing activity and polynucleotides encoding same
US8338121B2 (en) 2008-12-19 2012-12-25 Novozymes, Inc. Methods for determining cellulolytic enhancing activity of a polypeptide
WO2010088387A1 (en) 2009-01-28 2010-08-05 Novozymes, Inc. Polypeptides having beta-glucosidase activity and polynucleotides encoding same
EP2408314A1 (en) 2009-03-17 2012-01-25 Novozymes A/S Polypeptides having tyrosinase activity and polynucleotides encoding same
CN102597228A (en) 2009-10-23 2012-07-18 诺维信股份有限公司 Cellobiohydrolase variants and polynucleotides encoding same
CN106167796B (en) 2009-11-06 2019-06-04 诺维信股份有限公司 Composition for saccharified cellulosic material
WO2011057083A1 (en) 2009-11-06 2011-05-12 Novozymes, Inc. Polypeptides having xylanase activity and polynucleotides encoding same
WO2011057086A1 (en) 2009-11-06 2011-05-12 Novozymes, Inc. Polypeptides having cellobiohydrolase activity and polynucleotides encoding same
WO2012044836A1 (en) 2010-09-30 2012-04-05 Novozymes, Inc. Variants of polypeptides having cellulolytic enhancing activity and polynucleotides encoding same
BR112013007693B1 (en) 2010-09-30 2021-12-07 Novozymes, Inc VARIANT, ISOLATED POLYNUCLEOTIDE, RECOMBINANT MICROBIAL HOST CELL, METHODS FOR PRODUCING A VARIANT, TO PRODUCE A VARIANT HAVING CELLULOLYTIC ENHANCEMENT ACTIVITY, TO DEGRADE A CELLULOSIC MATERIAL, TO PRODUCE A FERMENTATION PRODUCT, TO FERMENT A CELLULOSIC, AND ELIGIBLE MATERIAL. COMPLETE BROTH OR CELL CULTURE COMPOSITION
DK2622069T3 (en) 2010-10-01 2016-02-22 Novozymes Inc Beta-glucosidasevarianter and polynucleotides encoding them
BR112013010129A2 (en) 2010-11-18 2016-07-05 Novozymes Inc isolated chimeric gh61 polypeptide, isolated polynucleotide, host cell, methods for producing a chimeric gh61 polypeptide, for degrading or converting a cellulostic material, for synthesizing a fermentation product, for fermenting a cellulosic material and for cleaning or washing a hard surface or clothing for washing, transgenic plant, part of plant or plant cell, use of chimeric gh61 polypeptide, detergent composition, and broth formulation or whole cell culture composition
WO2012103350A1 (en) 2011-01-26 2012-08-02 Novozymes A/S Polypeptides having cellobiohydrolase activity and polynucleotides encoding same
WO2012135719A1 (en) 2011-03-31 2012-10-04 Novozymes, Inc. Cellulose binding domain variants and polynucleotides encoding same
WO2012149192A1 (en) 2011-04-28 2012-11-01 Novozymes, Inc. Polypeptides having endoglucanase activity and polynucleotides encoding same
MX2013011827A (en) 2011-04-29 2014-01-08 Novozymes Inc Methods for enhancing the degradation or conversion of cellulosic material.
WO2013089889A2 (en) 2011-09-30 2013-06-20 Novozymes, Inc. Chimeric polypeptides having beta-glucosidase activity and polynucleotides encoding same
EP2794869B1 (en) 2011-12-20 2017-10-04 Novozymes, Inc. Cellobiohydrolase variants and polynucleotides encoding same
CN117716046A (en) * 2021-06-29 2024-03-15 诺维信公司 Process for producing fermentation products using fiber degrading enzymes in fermentation

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2563533B1 (en) * 1984-04-27 1986-08-22 Centre Nat Rech Scient METHOD FOR AMPLIFYING THE EXPRESSION OF A DETERMINED GENE IN SUBACTIVE BACILLUS AND STRAINS OBTAINED
AU8171087A (en) * 1986-10-27 1988-05-25 Whitehead Institute For Biomedical Research Gene amplification using retrotransposons
US4956288A (en) * 1988-04-22 1990-09-11 Biogen, Inc. Method for producing cells containing stably integrated foreign DNA at a high copy number, the cells produced by this method, and the use of these cells to produce the polypeptides coded for by the foreign DNA
DE69034135T3 (en) * 1989-12-22 2012-08-23 Laboratoires Serono S.A. DNA constructs to activate and alter the expression of endogenous genes
WO1992001800A1 (en) * 1990-07-20 1992-02-06 Chiron Corporation Method for integrative transformation of yeast using dispersed repetitive elements
US5641670A (en) * 1991-11-05 1997-06-24 Transkaryotic Therapies, Inc. Protein production and protein delivery
EP0966521A1 (en) * 1996-09-13 1999-12-29 Novo Nordisk Biotech, Inc. Cells having dna insertion mutations which produce altered amounts of a polypeptide

Also Published As

Publication number Publication date
EP1080210A2 (en) 2001-03-07
WO1999061651A3 (en) 2000-03-02
WO1999061651A2 (en) 1999-12-02
JP2002516116A (en) 2002-06-04
CN1307642A (en) 2001-08-08

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Legal Events

Date Code Title Description
MK6 Application lapsed section 142(2)(f)/reg. 8.3(3) - pct applic. not entering national phase