WO1997010328A3 - Compositions and method for modulation of gene expression in plants - Google Patents

Compositions and method for modulation of gene expression in plants Download PDF

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Publication number
WO1997010328A3
WO1997010328A3 PCT/US1996/011689 US9611689W WO9710328A3 WO 1997010328 A3 WO1997010328 A3 WO 1997010328A3 US 9611689 W US9611689 W US 9611689W WO 9710328 A3 WO9710328 A3 WO 9710328A3
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WO
WIPO (PCT)
Prior art keywords
plants
modulation
compositions
gene expression
acid molecule
Prior art date
Application number
PCT/US1996/011689
Other languages
French (fr)
Other versions
WO1997010328A2 (en
Inventor
Michael G Zwick
Brent E Edington
James A Mcswiggen
Patricia Ann Owens Merlo
Lining Guo
Thomas A Skokut
Scott A Young
Otto Folkerts
Donald J Merlo
Original Assignee
Ribozyme Pharm Inc
Dowelanco
Michael G Zwick
Brent E Edington
James A Mcswiggen
Patricia Ann Owens Merlo
Lining Guo
Thomas A Skokut
Scott A Young
Otto Folkerts
Donald J Merlo
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ribozyme Pharm Inc, Dowelanco, Michael G Zwick, Brent E Edington, James A Mcswiggen, Patricia Ann Owens Merlo, Lining Guo, Thomas A Skokut, Scott A Young, Otto Folkerts, Donald J Merlo filed Critical Ribozyme Pharm Inc
Priority to MX9800454A priority Critical patent/MX9800454A/en
Priority to JP9506989A priority patent/JPH11509733A/en
Priority to AU67617/96A priority patent/AU6761796A/en
Priority to BR9610402-3A priority patent/BR9610402A/en
Priority to EP96927999A priority patent/EP0842286A2/en
Publication of WO1997010328A2 publication Critical patent/WO1997010328A2/en
Publication of WO1997010328A3 publication Critical patent/WO1997010328A3/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C12N9/0083—Miscellaneous (1.14.99)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8218—Antisense, co-suppression, viral induced gene silencing [VIGS], post-transcriptional induced gene silencing [PTGS]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8245—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified carbohydrate or sugar alcohol metabolism, e.g. starch biosynthesis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8247—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified lipid metabolism, e.g. seed oil composition
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8255—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving lignin biosynthesis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1051—Hexosyltransferases (2.4.1)

Abstract

An enzymatic nucleic acid molecule with RNA cleaving activity, wherein said nucleic acid molecule modulates the expression of a gene in a plant. A transgenic plant comprising nucleic acids encoding for an enzymatic nucleic acid molecule with RNA cleaving activity, wherein said nucleic acid molecule modulates the expression of a gene in said plant.
PCT/US1996/011689 1995-07-13 1996-07-12 Compositions and method for modulation of gene expression in plants WO1997010328A2 (en)

Priority Applications (5)

Application Number Priority Date Filing Date Title
MX9800454A MX9800454A (en) 1995-07-13 1996-07-12 Compositions and method for modulation of gene expression in plants.
JP9506989A JPH11509733A (en) 1995-07-13 1996-07-12 Compositions and methods for regulating gene expression in plants
AU67617/96A AU6761796A (en) 1995-07-13 1996-07-12 Compositions and method for modulation of gene expression in plants
BR9610402-3A BR9610402A (en) 1995-07-13 1996-07-12 Compositions and method for modulating gene expression in plants.
EP96927999A EP0842286A2 (en) 1995-07-13 1996-07-12 Compositions and method for modulation of gene expression in plants

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US113595P 1995-07-13 1995-07-13
US60/001,135 1995-07-13

Publications (2)

Publication Number Publication Date
WO1997010328A2 WO1997010328A2 (en) 1997-03-20
WO1997010328A3 true WO1997010328A3 (en) 1997-05-15

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1996/011689 WO1997010328A2 (en) 1995-07-13 1996-07-12 Compositions and method for modulation of gene expression in plants

Country Status (8)

Country Link
EP (1) EP0842286A2 (en)
JP (1) JPH11509733A (en)
CN (1) CN1196091A (en)
AU (1) AU6761796A (en)
BR (1) BR9610402A (en)
CA (1) CA2226728A1 (en)
MX (1) MX9800454A (en)
WO (1) WO1997010328A2 (en)

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DE19601365A1 (en) 1996-01-16 1997-07-17 Planttec Biotechnologie Gmbh Nucleic acid molecules from plants encoding enzymes involved in starch synthesis
AU5918398A (en) * 1997-01-28 1998-08-18 Ribozyme Pharmaceuticals, Inc. Compositions and method for modulation of alkaloid biosynthesis and flower formation in plants
AU6591798A (en) * 1997-03-31 1998-10-22 Yale University Nucleic acid catalysts
US6656731B1 (en) 1997-09-22 2003-12-02 Max Planck Gesellschaft Zur Forderung Der Wissenschaften E.V. Nucleic acid catalysts with endonuclease activity
AU750947C (en) * 1997-09-22 2003-05-22 Max-Planck-Gesellschaft Zur Forderung Der Wissenschaften E.V. Nucleic acid catalysts with endonuclease activity
US6426185B1 (en) 1998-01-16 2002-07-30 Large Scale Biology Corporation Method of compiling a functional gene profile in a plant by transfecting a nucleic acid sequence of a donor plant into a different host plant in an anti-sense orientation
US6303848B1 (en) 1998-01-16 2001-10-16 Large Scale Biology Corporation Method for conferring herbicide, pest, or disease resistance in plant hosts
NZ507093A (en) * 1998-04-08 2003-08-29 Commw Scient Ind Res Org Methods and means for reducing the phenotypic expression of a nucleic acid of interest in a plant
US7008664B1 (en) * 1998-06-11 2006-03-07 E. I. Du Pont De Nemours And Company Method for improving the carcass quality of an animal
JP2002520051A (en) * 1998-07-20 2002-07-09 リボザイム・ファーマシューティカルズ・インコーポレーテッド Use of nucleic acid molecules as antiviral agents
DE69938144T2 (en) * 1998-07-21 2009-02-12 Dow Agrosciences Llc, Indianapolis ANTIBODY-MEDIATED DERIVATION REGULATION OF PLANT PROTEINS
ATE375712T1 (en) * 1998-08-04 2007-11-15 Cargill Inc PROMOTERS OF FATTY ACID DESATURASE FROM PLANTS
GB9914209D0 (en) 1999-06-17 1999-08-18 Danisco Process
EP1908843A1 (en) 1999-08-26 2008-04-09 Calgene LLC Nucleic acid sequences and methods of use for the production of plants with modified polyunsaturated fatty acids
US7157619B1 (en) 1999-08-30 2007-01-02 Monsanto Technology, L.L.C. Plant sterol acyltransferases
ATE476514T1 (en) * 2000-04-18 2010-08-15 Commw Scient Ind Res Org METHOD FOR MODIFYING COTTON OIL CONTENT
AU2001279007A1 (en) 2000-07-25 2002-02-05 Calgene Llc Nucleic acid sequences encoding beta-ketoacyl-acp synthase and uses thereof
IL154596A0 (en) 2000-08-25 2003-09-17 Basf Plant Science Gmbh Plant polynucleotides encoding novel prenyl proteases
US7125660B2 (en) 2000-09-13 2006-10-24 Archemix Corp. Nucleic acid sensor molecules and methods of using same
WO2002026028A2 (en) 2000-09-26 2002-04-04 The Regents Of The University Of California Characterization of phenylalanine ammonia-lyase (pal) gene in wounded lettuce
AU2004254373B2 (en) * 2003-06-27 2009-11-19 Monsanto Technology, Llc Elevation of oil levels in plants
US7820883B2 (en) 2006-03-15 2010-10-26 Dow Agrosciences Llc Resistance to auxinic herbicides
EP2044206A2 (en) 2006-06-13 2009-04-08 Agrinomics, LLC Generation of plants with improved pathogen resistance
WO2008000511A2 (en) * 2006-06-30 2008-01-03 Freie Universität Berlin Pektin methyltransferases and their applications
BR102012008162B1 (en) * 2012-04-09 2018-08-14 Empresa Brasileira De Pesquisa Agropecuária - Embrapa compositions and methods for modifying the expression of genes of interest

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EP0360257A2 (en) * 1988-09-20 1990-03-28 The Board Of Regents For Northern Illinois University RNA catalyst for cleaving specific RNA sequences
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Also Published As

Publication number Publication date
AU6761796A (en) 1997-04-01
MX9800454A (en) 1998-04-30
WO1997010328A2 (en) 1997-03-20
EP0842286A2 (en) 1998-05-20
BR9610402A (en) 2000-01-11
CA2226728A1 (en) 1997-03-20
CN1196091A (en) 1998-10-14
JPH11509733A (en) 1999-08-31

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