WO1999012950A3 - Improvements in or relating to stability of plant starches - Google Patents

Improvements in or relating to stability of plant starches Download PDF

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Publication number
WO1999012950A3
WO1999012950A3 PCT/GB1998/002665 GB9802665W WO9912950A3 WO 1999012950 A3 WO1999012950 A3 WO 1999012950A3 GB 9802665 W GB9802665 W GB 9802665W WO 9912950 A3 WO9912950 A3 WO 9912950A3
Authority
WO
WIPO (PCT)
Prior art keywords
relating
stability
nucleic acid
acid sequence
plant starches
Prior art date
Application number
PCT/GB1998/002665
Other languages
French (fr)
Other versions
WO1999012950A2 (en
WO1999012950A9 (en
Inventor
Stephen Alan Jobling
Gerhard Peter Schwall
Roger John Westcott
Original Assignee
Nat Starch Chem Invest
Stephen Alan Jobling
Gerhard Peter Schwall
Roger John Westcott
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Nat Starch Chem Invest, Stephen Alan Jobling, Gerhard Peter Schwall, Roger John Westcott filed Critical Nat Starch Chem Invest
Priority to CA002302639A priority Critical patent/CA2302639A1/en
Priority to EP98941593A priority patent/EP1009751A2/en
Priority to AU89911/98A priority patent/AU8991198A/en
Publication of WO1999012950A2 publication Critical patent/WO1999012950A2/en
Publication of WO1999012950A3 publication Critical patent/WO1999012950A3/en
Publication of WO1999012950A9 publication Critical patent/WO1999012950A9/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2451—Glucanases acting on alpha-1,6-glucosidic bonds
    • C12N9/2457—Pullulanase (3.2.1.41)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8245—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified carbohydrate or sugar alcohol metabolism, e.g. starch biosynthesis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2451—Glucanases acting on alpha-1,6-glucosidic bonds
    • C12N9/246—Isoamylase (3.2.1.68)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01041—Pullulanase (3.2.1.41)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01068—Isoamylase (3.2.1.68)

Abstract

Disclosed is a nucleic acid sequence obtainable from potato plants and carrying at least a portion of an isoamylase enzyme gene, together with constructs and host cells comprising the nucleic acid sequence, and methods of using the nucleic acid sequence.
PCT/GB1998/002665 1997-09-06 1998-09-04 Improvements in or relating to stability of plant starches WO1999012950A2 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
CA002302639A CA2302639A1 (en) 1997-09-06 1998-09-04 Improvements in or relating to stability of plant starches
EP98941593A EP1009751A2 (en) 1997-09-06 1998-09-04 Improvements in or relating to stability of plant starches
AU89911/98A AU8991198A (en) 1997-09-06 1998-09-04 Improvements in or relating to stability of plant starches

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB9718863.5 1997-09-06
GBGB9718863.5A GB9718863D0 (en) 1997-09-06 1997-09-06 Improvements in or relating to stability of plant starches

Publications (3)

Publication Number Publication Date
WO1999012950A2 WO1999012950A2 (en) 1999-03-18
WO1999012950A3 true WO1999012950A3 (en) 1999-05-06
WO1999012950A9 WO1999012950A9 (en) 1999-06-10

Family

ID=10818600

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB1998/002665 WO1999012950A2 (en) 1997-09-06 1998-09-04 Improvements in or relating to stability of plant starches

Country Status (5)

Country Link
EP (1) EP1009751A2 (en)
AU (1) AU8991198A (en)
CA (1) CA2302639A1 (en)
GB (1) GB9718863D0 (en)
WO (1) WO1999012950A2 (en)

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