WO2015143783A1 - 一种天然调理养生酵素及其制造方法 - Google Patents
一种天然调理养生酵素及其制造方法 Download PDFInfo
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Classifications
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/135—Bacteria or derivatives thereof, e.g. probiotics
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Definitions
- the present invention relates to the technical field of enzymes, and more particularly to a natural conditioning health enzyme and a method of producing the same.
- Enzyme is a kind of expression after commercialization of an enzyme product, and is a catalytically active protein. Regardless of animals or plants, the activities of all living things require the participation of enzymes. Not only does the human body not only digest and absorb the food ingested every day, the operation of various organs in the body, but also the metabolism of the cells are inseparable from enzymes, and any substance that breaks down into the body needs enzyme activity to complete. At present, with the continuous advancement of society, people's health awareness is getting stronger and stronger. It is very beneficial to take plant enzymes made from pure natural plants to supplement the enzymes necessary for the human body.
- the natural conditioning health enzyme comprises the following raw materials in parts by mass: 1 to 80 parts of the fermentation broth, 1 to 30 parts of probiotics, 1 to 30 parts of enzyme, 1 to 10 parts of Chinese herbal medicine, 1 to 30 parts of fruit, 1 to 30 servings of vegetables, 1 ⁇ 30 parts of edible fungi, 1 to 30 parts of edible algae, 1 to 30 parts of oligosaccharides and 1 to 30 parts of honey; wherein, the pre-fermentation liquid is determined by the mass fraction of the previously fermented raw material, 1 to 50 parts by mass of corn ear 1 to 15 parts by mass of wheat, 1 to 30 parts by mass of stalked rice, 1 to 50 parts by mass of brown rice, 1 to 50 parts by mass of rice bran, 1 to 50 parts by mass of black beans, 1 to 10 parts by mass of black glutinous rice and 1 to 10 parts by mass Black sesame is the main component obtained by fermentation extraction of fermenting bacteria.
- the natural conditioning health enzyme comprises the following raw material components: 1 to 80 parts of the fermentation broth, 1 to 30 parts of probiotics, 1 to 30 parts of enzyme, 1 to 10 parts of Chinese herbal medicine, 1 ⁇ 30 parts of fruit, 1 to 30 parts of vegetables, 1 to 30 parts of edible fungi, 1 to 30 parts of edible algae, 1 to 30 parts of maltose and 1 to 30 parts of honey, wherein the pre-fermentation liquid is a raw material of the previous fermentation liquid
- the mass fraction, 1 to 50 parts by mass of corn ear, 1 to 15 parts by mass of wheat, 1 to 30 parts by mass of stalked rice, 1 to 50 parts by mass of brown rice, 1 to 50 parts by mass of rice bran, 1 to 50 parts by mass of black beans, 1 to 10 parts by mass of black glutinous rice and 1 to 10 parts by mass of black sesame are mainly composed of fermentation bacteria.
- the enzyme is a saccharification enzyme, an acid amylase, an ex-amylase, a ⁇ -amylase, a ⁇ -amylase, an isoamylase, a lipase, a banana enzyme, a pineapple enzyme, a papain, a protease.
- pepsin pepsin, elastase, plasmin, calculus, phytase, xylanase, cellulase, hemicellulase, ⁇ -glucanase, oxalate decarboxylase, polygalactosaldehyde Acidase, pectin esterase, pectin hydrolase, lactase, maltase, beta-glucosidase, anthocyaninase, lysozyme, urokinase, streptokinase, nattokinase, superoxide dismutase, Hydrogenase, coenzyme, coenzyme Q-10, hyaluronidase, collagenase, chitosan hydrolase, alcohol dehydrogenase, acetaldehyde dehydrogenase, adenosine triphosphatase, methionine aden
- the probiotics are brewer's yeast, membrane acetic acid bacteria, cellulose acetate, lactic acid bacteria, Lactobacillus bulgaricus, Lactobacillus brevis, Streptococcus thermophilus, Lactobacillus acidophilus, Bifidobacterium, coagulation One or at least two of Bacillus lactic acid bacteria, Bifidobacterium lactis, and Monascus.
- the Chinese herbal medicine is Cordyceps, Polygonatum, Ganoderma Lucidum, Ginseng, Schisandra, Lotus Seed, Cassia, Angelica, Sanqi, Polygonum, Rhododendron, Pine Pollen, Rhodiola, Codonopsis, Pentaphyllum, White Flower Snake Grass, grass, Centella asiatica, motherwort, licorice, wheatgrass, pasture, plantain, alfalfa, Ginger, Chuanxiong, Poria, Northern Bupleurum, Rehmannia glutinosa, Atractylodes, Chenpi, Jujube, Peony, bamboo Leaf, Loquat Leaf, Chuanqiye, Lotus Leaf, Mulberry Leaf, Astragalus, Sea Cucumber, Tortoiseshell, Lily, Polygonatum, One or at least two of Gynostemma pentaphyllum, Coix seed, Prunus armenii, Radix Puerariae,
- the fruit is bayberry, ebony, hawthorn, persimmon, peach, plum, coconut, cantaloupe, watermelon, papaya, melon, cantaloupe, pineapple, carambola, cherry, lemon, alfalfa, sugar cane, lotus, One or at least two of longan, lychee, grape, jackfruit, mulberry, strawberry, grapefruit, orange, orange, kumquat, guava, dragon fruit, banana, mango, kiwi, apple, jujube, and chayote; Vegetables for potato, carrot, white radish, cabbage, bean sprouts, purslane, yam, lotus root, burdock, sweet potato, loofah, bitter gourd, winter melon, celery, okra, chrysanthemum, taro, water spinach, leeks, sage, kale , one or at least two of bitter herbs, broccoli, asparagus
- the pre-fermentation liquid refers to a fermentation obtained by fermentation of fermenting bacteria in an aqueous environment using corn ear, wheat, stem rice, brown rice, rice bran, black bean, black glutinous rice and black sesame as raw materials.
- the liquid component after extraction can carry out any of the fermenting bacteria well known to those skilled in the art, such as yeast, Aspergillus or Aspergillus oryzae.
- the amount of water to be added the principle of not affecting the propagation and metabolism of the fermenting bacteria can be obtained by a simple test or a known technique.
- the amount of water suitable for use in the formulation of this embodiment may be from 50 to 96 parts by mass.
- Another aspect of the present invention provides a method for producing the above enzyme, which has an integrated conditioning effect.
- a method of making an enzyme as described above comprising the steps of:
- the fermenting bacteria are yeast, Aspergillus or Aspergillus oryzae, and the inoculating amount of the fermenting bacteria is 5 to 35% ; the yeast is first inoculated into a 250 ml flask for cultivation, and then inoculated.
- Aspergillus or Aspergillus strains are inoculated into a 300ml flask for cultivation, then inoculated into a 1000ml flask for cultivation, and then transferred into the fermentation vessel; Before the cultivation, steam sterilization is carried out under the pressure of O.lMpa for 20 to 30 minutes, and the culture condition is: 25 ⁇ 33 °0 constant temperature culture 721.
- the step (1) includes the raw material of the pre-manufacturing fermentation liquid including water impregnation and steaming, and the water is impregnated with water at a temperature of 25 to 30 ° C, and the water has a pH of 7 to 8
- the water hardness is 2 ⁇ 6mmol/L, the iron ion content in the water is not more than 0.5mg/L, and the immersion time is 24 ⁇ 72 hours; the steaming material is steamed at atmospheric pressure for 15 ⁇ 60min.
- the temperature of the fermentation in the step (1) is 23 to 38 ° C, and the time is 30 to 60 days; the fermentation is carried out during the fermentation, and the specific operation mode is: the temperature at the start of fermentation is controlled at 23 At ⁇ 30 ° C, the first stirring is carried out after 10 hours of fermentation.
- the temperature of the fermentation after the first stirring is controlled at 37 to 38 ° C; after 4 to 6 hours, the second stirring is carried out, and the second fermentation is followed by fermentation.
- the temperature was controlled at 31 ⁇ 33 ° C; and after 3 to 4 hours with stirring and then for the third time, the third was stirred fermentation temperature was controlled at 29 ⁇ 31 ° C; before and after 3 to 4 hours for the first four stirring.
- the step (3) further includes disinfection, and the sterilization is performed in a coiled tube heat exchanger or a Bath sterilization tank, the sterilization temperature is 75 to 90 ° C, and the sterilization time is 4 to 4 10min.
- the inoculum amount of the fermenting bacteria refers to the ratio of the volume of the seed liquid to be transferred to the volume of the medium after inoculation.
- the fermenting bacteria can be cultured in multiple stages before inoculation into the fermentation vessel. As for the nutrient solution required for the above fermentation broth culture, it is common knowledge in the art, and details are not described herein.
- the enzyme of the present invention combines vegetables, fruits, edible fungi, edible algae, and Chinese medicinal materials with an enzyme, and is obtained by extracting by fermenting the beneficial bacteria.
- the compound Chinese herbal medicine can enhance the comprehensiveness of conditioning; compounding to maintain the body's normal essential enzymes and compounding natural and natural fruit and vegetable algae, no toxic side effects.
- the enzyme of the invention adopts enzymes and plants to ferment by beneficial bacteria, imparts extraordinary functions to ordinary plants, can be directly eaten, and can also be added to beverages and foods, thereby achieving the health and health effects of providing natural and comprehensive conditioning to people. Specific real
- Example 1 Fermentation consisting of 2 parts by mass of corn, 1 part by mass of wheat, 3 parts by mass of stalked rice, 1 part by mass of brown rice, 1 part by mass of rice bran, 2 parts by mass of black beans, 2 parts by mass of black glutinous rice, and 1 part by mass of black sesame
- the raw material is placed in a container, 50 to 96 parts by mass of water is added, and after thorough mixing, it is immersed at a temperature of 25 to 30 ° C for 24 to 72 hours to ensure a moisture absorption amount of 25 to 30%.
- the pH of the water used for the impregnation is 7 to 8, the hardness is 2 to 6 mmol/L, the iron ion content is not more than 0.5 mg/L, and the appearance is clear and transparent, colorless, and has no peculiar smell.
- the impregnated fermented raw material is placed in a steaming machine for steaming, and the raw material is steamed under normal pressure for 15 to 30 minutes, and rapidly cooled under a closed condition to obtain a fermented clinker.
- Pre-fermentation stage The fermented clinker is put into a large tank, and the yeast strain cultured twice in a 250 ml triangle bottle and a 500 ml triangle bottle is put into a large tank according to the inoculum amount of 5 to 35%, and will be successively passed through a 300 ml triangle bottle and 1000 ml.
- the Aspergillus or Aspergillus oryzae strain cultured twice in a triangular flask is put into a large tank according to the inoculum amount of 5 to 35%, and the yeast strain, the Aspergillus or Aspergillus strain and the fermented clinker are mixed and stirred uniformly. Enter the stage of pre-fermentation.
- the temperature at the start of fermentation is controlled at 23 to 30 ° C, and the first stirring is carried out after 10 hours of fermentation.
- the temperature of the fermentation after the first stirring is controlled at 37 to 38 ° C ; after 4 to 6 hours, the first is carried out.
- the temperature of the fermentation after the second stirring is controlled at 31 ⁇ 33 °C ; after 3 ⁇ 4 hours, the third stirring is performed, and the temperature of the fermentation after the third stirring is controlled at 29 ⁇ 31 °C ;
- the fourth stirring is carried out.
- the temperature of the fermentation after the fourth stirring is controlled at 27 to 30 ° C ; thereafter, each morning and evening is stirred once, and during the fermentation, the temperature is gradually lowered to 23 to 30. °C. Fermentation mash was obtained after 30 days of fermentation. The fermented mash is extracted to obtain a pre-fermentation liquid.
- Post-fermentation stage Fermentation raw material according to the following mass ratio, that is, 1 part by mass of the fermentation broth, 0.1 part by mass of Lactobacillus bulgaricus, 0.2 part by mass of Lactobacillus plantum, 0.2 part by mass of Streptococcus thermophilus, 0.3 part by mass of yeast 0.1 part by mass of Lactobacillus acidophilus, 0.5 part by mass of Bifidobacterium, 0.6 part by mass of superoxide dismutase, 0.15 part by mass of lysozyme, 0.1 part by mass of protease, 0.15 part by mass of coenzyme Q-10, 0.1 part by mass of sarcophagus, 0.2 The mass of Chuanxiong, 0.1 parts by mass of medlar, 0.1 parts by mass of Hedyotis diffusa, 0.1 parts by mass of Scutellaria barbata, 0.04 parts by mass of parsnip, 0.06 parts by mass of honeysuckle, 0.08 parts by mass
- the fermentation temperature is 10 to 30 ° C
- the fermentation time is 25 days or more
- the fermentation broth is extracted to obtain the fermentation broth.
- the post-fermentation liquid is placed in a jar or a large tank, and is aged for at least 80 days, and the temperature of the altar is 10 to 30 °C.
- the supernatant fermentation broth after the altar is sent to the coil tube heat exchanger or the Bass sterilization tank for low temperature sterilization; the sterilization temperature is controlled at 75 ⁇ 90 ° C, the sterilization time is 4 ⁇ 10 min ; Bottling, sealing, and obtaining enzyme products.
- Example 2 In addition to the fermentation raw material, 50 parts by mass of corn, 15 parts by mass of wheat, 30 parts by mass of stalked rice, 15 parts by mass of brown rice, 15 parts by mass of rice bran, 50 parts by mass of black beans, 10 parts by mass of black glutinous rice, and 10 parts by mass of black sesame
- the composition and the raw material in the post-fermentation stage are 80 parts by mass of the pre-fermentation liquid, 6 parts by mass of the coagulating lactic acid bacteria, 3 parts by mass of F. faecalis, 4 parts by mass of Lactobacillus acidophilus, 7 parts by mass of Bifidobacterium, 4 parts by mass.
- Amylase 5 parts by mass of lipase, 6 parts by mass of protease, 15 parts by mass of cellulase, 4 parts by mass of Chuanxiong, 4 parts by mass of parsnip, 6 parts by mass of honeysuckle, 8 parts by mass of rhubarb, 3 parts by mass of plantain, 5 Quality of Achyranthes, 1 part by mass of lemon, 2 parts by mass of pineapple, 2 parts by mass of grapes, 3 parts by mass of apple, 2 parts by mass of cantaloupe, 1 part by mass of burdock, 1 part by mass of potato, 1 part by mass of pumpkin, 1 part by mass of eggplant , 0.5 parts by mass of melon, 2 parts by mass of carrot, 0.5 part by mass of broccoli, 1 part by mass of celery, 0.2 parts by mass of spinach, 0.3 parts by mass of kale, 0.3 parts by mass of bitter gourd, 0.2 parts by mass of green pepper, 1 quality Parts of soybean sprouts, 3 parts by mass of shiitake mushrooms, 2 parts
- Example 3 In addition to the fermentation raw material, 40 parts by mass of corn, 7 parts by mass of wheat, 15 parts by mass of stalked rice, 8 parts by mass of brown rice, 7 parts by mass of rice bran, 25 parts by mass of black beans, 6 parts by mass of black glutinous rice, and 5 parts by mass of black sesame
- the composition and the raw material in the post-fermentation stage are 40 parts by mass of the pre-fermentation liquid, 2 parts by mass of Lactobacillus bulgaricus, 2 parts by mass of Streptococcus thermophilus, 1 part by mass of Bifidobacterium breve, 1 part by mass of Bifidobacterium, 1 part by mass.
- Example 4 In addition to the fermentation raw material, 20 parts by mass of corn, 3 parts by mass of wheat, 8 parts by mass of stalked rice, 4 parts by mass of brown rice, 3 parts by mass of rice bran, 12 parts by mass of black beans, 3 parts by mass of black glutinous rice, and 2 parts by mass of black sesame
- the composition and the raw material in the post-fermentation stage are 10 parts by mass of the pre-fermentation liquid, 3 parts by mass of Bifidobacterium lactis, 2 parts by mass of brewer's yeast, 0.5 parts by mass of Streptococcus thermophilus, 0.5 parts by mass of F. faecalis, and 1 part by mass.
- Soybean Germ Isoflavone Enzyme 0.6 parts by mass of lipase, 2 parts by mass of protease, 3 parts by mass of cellulase, 0.4 parts by mass of coenzyme Q10, 0.5 parts by mass of motherwort, 0.3 parts by mass of lily, 0.2 parts by mass of rose, 0.6 parts by mass of chrysanthemum 0.3 parts by mass of rose, 0.2 parts by mass of jasmine, 0.2 parts by mass of peony bark, 0.2 parts by mass of eucalyptus leaves, 0.4 parts by mass of angelica, 0.2 parts by mass of walnut, 0.5 parts by mass of lotus seeds, 0.3 parts by mass of enamel, 0.1 parts by mass of polygonatum, 0.3 Parts by mass of Huang Jing, 0.6 parts by mass of hawthorn, 0.8 parts by mass of medlar, 0.3 parts by mass of orange peel, 0.5 parts by mass of aloe vera, 0.2 parts by mass of jaundice,
- Example 5 In addition to the fermentation raw material, 30 parts by mass of corn, 4 parts by mass of wheat, 7 parts by mass of stalked rice, 3 parts by mass of brown rice, 2 parts by mass of rice bran, 20 parts by mass of black beans, 5 parts by mass of black glutinous rice, and 3 parts by mass of black sesame
- the composition, and the raw material in the post-fermentation stage are 15 parts by mass of the fermentation broth, 2 parts by mass of brewer's yeast, 0.1 a mass of Lactobacillus acidophilus, 0.2 parts by mass of S. thermophilus, 0.7 parts by mass of Monascus, 0.4 parts by mass of F.
- faecalis 0.6 parts by mass of Bifidobacterium, 0.5 parts by mass of lipase, 1 part by mass of protease, 0.2 parts by mass of rosemary, 0.4 parts by mass of aloe vera, 0.4 parts by mass of olean, 0.3 parts by mass of schisandra, 0.4 parts by mass of astragalus, 0.6 parts by mass of Sophora flavescens, 0.7 parts by mass of angelica, 0.4 parts by mass of perilla, 0.2 parts by mass of calamus 0.2 parts by mass of lotus leaf, 0.2 parts by mass of rose, 0.2 parts by mass of jasmine, 0.3 parts by mass of Chuanxiong, 0.3 parts by mass of atractylodes, 0.5 parts by mass of strontium, 0.2 parts by mass of Alisma, 0.2 parts by mass of plantain, 0.3 parts by mass of summer Withered grass, 0.4 parts by mass of asparagus, 0.6 parts by mass of medlar, 0.5
- Example 6 In addition to the fermentation raw material, 40 parts by mass of corn, 10 parts by mass of wheat, 22 parts by mass of stalked rice, 20 parts by mass of brown rice, 8 parts by mass of rice bran, 38 parts by mass of black beans, 8 parts by mass of black glutinous rice, and 6 parts by mass of black sesame
- the composition, and the raw material in the post-fermentation stage are 60 parts by mass of the preceding fermentation broth, 12 parts by mass of brewer's yeast, 8 parts by mass of Bifidobacterium lactis, 10 parts by mass of calcitonase, 7 parts by mass of oxalic acid decarboxylase, 1 part by mass of melon skin, 5 Quality of Chuanxiong, 3 parts by mass of walnut, 2 parts by mass of phoenix grass, 1 part by mass of sea gold sand, 1 part by mass of cucumber vine, 1 part by mass of pepper, 4 parts of plantain, 2 parts of rose, 2 parts of bergamot Melon, 3 parts
- Example 7 In addition to the fermentation raw material, 35 parts by mass of corn, 6 parts by mass of wheat, 10 parts by mass of stalked rice, 9 parts by mass of brown rice, 5 parts by mass of rice bran, 32 parts by mass of black beans, 7 parts by mass of black glutinous rice, and 4 parts by mass of black sesame
- the composition, and the raw material in the post-fermentation stage are 55 parts by mass of the preceding fermentation broth, 6 parts by mass of brewer's yeast, 5 parts by mass of cellulose acetate, 4 parts by mass of lactic acid bacteria, 8 parts by mass of glutamic acid protease, 6 parts by mass of amylase, 4 masses.
- Nattokinase 12 parts by mass of natto, 2 parts by mass of Rhodiola, 1 part by mass of hawthorn, 3 parts by mass of salvia miltiorrhiza, 1 part by mass of pine, 1 part by mass of phytosterol, 3 parts by mass of banana, 1 part by mass Mango, 2 parts by mass of apple, 0.3 parts by mass of lemon, 0.4 parts by mass of kiwi, 0.3 parts by mass of mulberry, 1 part by mass of carrot, 0.8 parts by mass of lettuce, 0.3 parts by mass of amaranth, 0.1 part by mass of cucumber, 0.2 parts by mass of tomato, 0.1 part by mass Sweet potato, 0.2 parts by mass of loofah, 0.1 parts by mass of melon, 0.2 parts by mass of celery, 1 part by mass of shiitake mushrooms, 0.6 parts by mass of Flammulina velutipes, 0.6 parts by mass of black fungus, 0.4 parts by mass of Coprinus comatus, 0. Except for 4
- Example 8 In addition to the fermentation raw material, 45 parts by mass of corn, 8 parts by mass of wheat, 28 parts by mass of stalked rice, 14 parts by mass of brown rice, 10 parts by mass of rice bran, 32 parts by mass of black beans, 8 parts by mass of black glutinous rice, and 7 parts by mass of black sesame
- the composition and the raw material in the post-fermentation stage are 70 parts by mass of the preceding fermentation broth, 6 parts by mass of Lactobacillus acidophilus, 8 parts by mass of Bifidobacterium, 8 parts by mass of protease, 7 parts by mass of cellulase, 0.1 parts by mass of bamboo shoots, 0.2 mass. ⁇ ⁇ , 0.1 parts by mass of ginger, 0.2 parts by mass of bismuth, 0.7 parts by mass of bismuth, 3 parts by mass of Ganoderma lucidum, 0.1 parts by mass of safflower, 0.2 parts by mass of notoginseng, 2 parts by mass of Chuanxiong, 0.2 parts by mass
- Example 9 In addition to the fermentation raw material, 30 parts by mass of corn, 5 parts by mass of wheat, 15 parts by mass of stalked rice, 5 parts by mass of brown rice, 3 parts by mass of rice bran, 25 parts by mass of black beans, 5 parts by mass of black glutinous rice, and 4 parts by mass of black sesame
- the composition and the raw material in the post-fermentation stage are 45 parts by mass of the pre-fermentation liquid, 7 parts by mass of lactic acid bacteria, 4 parts by mass of Monascus, 5 parts by mass of Saccharomyces cerevisiae, 8 parts by mass of glutamic acid decarboxylase, 2 parts by mass of lysozyme, 3 parts by mass of protease, 5 parts by mass of amylase, 4 parts by mass of lipase, 0.5 parts by mass of citrus, 0.1 parts by mass of ginger, 0.1 parts by mass of ruthenium, 0.7 parts by mass of ruthenium, 3 parts by mass of Ganoderma lucidum,
- Demyella 4 parts by mass of Lactobacillus acidophilus, 7 parts by mass of Bifidobacterium, 4 parts by mass of amylase, 5 parts by mass of lipase, 6 parts by mass of protease, 15 parts by mass of cellulase, 4 parts by mass of Chuanxiong, 4 masses Sesame grass, 6 parts by mass of honeysuckle, 8 parts by mass of rhubarb, 3 parts by mass of plantain, 5 parts of Sichuan achyranthes, 1 part by mass of lemon, 2 parts by mass of pineapple, 2 parts by mass of grape, 3 quality An apple, 2 parts by mass of cantaloupe, 1 part by mass of burdock, 1 part by mass of potato, 1 part by mass of pumpkin, 1 part by mass of eggplant, 0.5 part by mass of melon, 2 parts by mass of carrot, 0.5 part by mass of broccoli, 1 part by mass of celery, 0.2 parts by mass of spinach, 0.3 parts by mass of kale, 0.3 parts by mass
- Example 11 In addition to the fermentation raw material, 50 parts by mass of corn, 15 parts by mass of wheat, 30 parts by mass of stalked rice, 15 parts by mass of brown rice, 15 parts by mass of rice bran, 50 parts by mass of black beans, 10 parts by mass of black glutinous rice, and 10 parts by mass of black sesame
- the composition, and the raw material in the post-fermentation stage are 80 parts by mass of the pre-fermentation liquid, 6 parts by mass of the coagulating lactic acid bacteria, 3 parts by mass of F. faecalis, 4 parts by mass of Lactobacillus acidophilus, 7 parts by mass of Bifidobacterium, 4 parts by mass.
- Amylase 5 parts by mass of lipase, 6 parts by mass of protease, 15 parts by mass of cellulase, 4 parts by mass of Chuanxiong, 4 parts by mass of parsnip, 6 parts by mass of honeysuckle, 8 parts by mass of rhubarb, 3 parts by mass of plantain, 5 Quality of Achyranthes, 1 part by mass of lemon, 2 parts by mass of pineapple, 2 parts by mass of grapes, 3 parts by mass of apple, 2 parts by mass of cantaloupe, 1 part by mass of burdock, 1 part by mass of potato, 1 part by mass of pumpkin, 1 part by mass of eggplant 0.5 parts by mass of melon, 2 parts by mass of carrot, 0.5 part by mass of broccoli, 1 part by mass of celery, 0.2 parts by mass of spinach, 0.3 parts by mass of kale, 0.3 parts by mass of bitter gourd, 0.2 parts by mass of green pepper, 1 A portion of soybean sprouts, 3 parts by mass of shiitake mushrooms, 2 parts by mass of
- Example 12 In addition to the fermentation raw material, 50 parts by mass of corn, 15 parts by mass of wheat, 30 parts by mass of stalked rice, 15 parts by mass of brown rice, 15 parts by mass of rice bran, 50 parts by mass of black beans, 10 parts by mass of black glutinous rice, and 10 parts by mass of black sesame
- the composition, and the raw material in the post-fermentation stage are 80 parts by mass of the pre-fermentation liquid, 6 parts by mass of the coagulating lactic acid bacteria, 3 parts by mass of F. faecalis, 4 parts by mass of Lactobacillus acidophilus, 7 parts by mass of Bifidobacterium, 4 parts by mass.
- Amylase 5 parts by mass of lipase, 6 parts by mass of protease, 15 parts by mass of cellulase, 4 parts by mass of Chuanxiong, 4 parts by mass of parsnip, 6 parts by mass of honeysuckle, 8 parts by mass of rhubarb, 3 parts by mass of plantain, 5 Quality of Achyranthes, 1 part by mass of lemon, 2 parts by mass of pineapple, 2 parts by mass of grapes, 3 parts by mass of apple, 2 parts by mass of cantaloupe, 1 part by mass of burdock, 1 part by mass of potato, 1 part by mass of pumpkin, 1 part by mass of eggplant , 0.5 parts by mass of melon, 2 parts by mass of carrot, 0.5 part by mass of broccoli, 1 part by mass of celery, 0.2 parts by mass of spinach, 0.3 parts by mass of kale, 0.3 parts by mass of bitter gourd, 0.2 parts by mass of green pepper, 1 part by mass of yellow Bud, 3 parts by mass of mushrooms, Flammulina parts by
- Example 13 In addition to the fermentation raw material, 50 parts by mass of corn, 15 parts by mass of wheat, 30 parts by mass of stalked rice, 15 parts by mass of brown rice, 15 parts by mass of rice bran, 50 parts by mass of black beans, 10 parts by mass of black glutinous rice, and 10 parts by mass of black sesame
- the composition, and the raw material in the post-fermentation stage are 80 parts by mass of the pre-fermentation liquid, 6 parts by mass of the coagulating lactic acid bacteria, 3 parts by mass of F. faecalis, 4 parts by mass of Lactobacillus acidophilus, 7 parts by mass of Bifidobacterium, 4 parts by mass.
- Amylase 5 parts by mass of lipase, 6 parts by mass of protease, 15 parts by mass of cellulase, 4 parts by mass of Chuanxiong, 4 parts by mass of parsnip, 6 parts by mass of honeysuckle, 8 parts by mass of rhubarb, 3 parts by mass of plantain, 5 Quality of Achyranthes, 1 part by mass of lemon, 2 parts by mass of pineapple, 2 parts by mass of grapes, 3 parts by mass of apple, 2 parts by mass of cantaloupe, 1 part by mass of burdock, 1 part by mass of potato, 1 part by mass of pumpkin, 1 part by mass of eggplant 0.5 parts by mass of melon, 2 parts by mass of carrot, 0.5 part by mass of broccoli, 1 part by mass of celery, 0.2 parts by mass of spinach, 0.3 parts by mass of kale, 0.3 parts by mass of bitter gourd, 0.2 parts by mass of green pepper, 1 A portion of soybean sprouts, 3 parts by mass of shiitake mushrooms, 2 parts by mass of
- Example 14 In addition to the fermentation raw material, 40 parts by mass of corn, 7 parts by mass of wheat, 15 parts by mass of stalked rice, 8 parts by mass of brown rice, 7 parts by mass of rice bran, 25 parts by mass of black beans, 6 parts by mass of black glutinous rice, and 5 parts by mass of black sesame
- the composition and the raw material in the post-fermentation stage are 40 parts by mass of the pre-fermentation liquid, 2 parts by mass of Lactobacillus bulgaricus, 2 parts by mass of Streptococcus thermophilus, 1 part by mass of Bifidobacterium breve, 1 part by mass of Bifidobacterium, 1 part by mass.
- Catalase 3 parts by mass of lipase, 4 parts by mass of protease, 6 parts by mass of cellulase, 1 part by mass of superoxide dismutase, 6 parts by mass of wheat grass, 3 parts by mass of stevia, 2 parts by mass of barley malt, 4 parts by mass of bismuth, 2 parts by mass of grapes, 1 part by mass of kumquat, 0.8 parts by mass of sweet orange, 0.6 parts by mass of citrus, 0.4 parts by mass of Shatian pomelo, 0.2 parts by mass of green papaya, 0.3 parts by mass of long bean, 0.2 parts by mass of green beans 0.2 parts by mass of loofah, 0.2 parts by mass of cucumber, 0.1 parts by mass of melon, 0.1 parts by mass of bitter gourd, 0.4 parts by mass of pumpkin, 0.3 parts by mass of sweet potato leaves, 0.2 parts by mass of burdock, 0.6 parts by mass of yam, 0.4 parts by mass of lettuce 0.7 parts by mass of tomatoes,
- Example 15 In addition to the fermentation raw material, 30 parts by mass of corn, 4 parts by mass of wheat, 7 parts by mass of stalked rice, 3 parts by mass of brown rice, 2 parts by mass of rice bran, 20 parts by mass of black beans, 5 parts by mass of black glutinous rice, and 3 parts by mass of black sesame
- the composition, and the raw material in the post-fermentation stage are 15 parts by mass of the preceding fermentation broth, 2 parts by mass of brewer's yeast, 0.1 parts by mass of Lactobacillus acidophilus, 0.2 parts by mass of Streptococcus thermophilus, 0.7 parts by mass of Monascus, 0.4 parts by mass.
- faecalis 0.6 parts by mass of Bifidobacterium, 0.5 parts by mass of lipase, 1 part by mass of protease, 0.2 parts by mass of rosemary, 0.4 parts by mass of aloe, 0.4 parts by mass of olean, 0.3 parts by mass of schisandra, 0.4 parts by mass Astragalus, 0.6 parts by mass of Sophora flavescens, 0.7 parts by mass of Angelica, 0.4 parts by mass of perilla, 0.2 parts by mass of calamus, 0.2 parts by mass of lotus leaf, 0.2 parts by mass of rose, 0.2 parts by mass of jasmine, 0.3 parts by mass of Chuanxiong, 0.3 parts by mass of Atractylodes , 0.5 parts by mass of bismuth, 0.2 parts by mass of Alisma, 0.2 parts by mass of plantain, 0.3 parts by mass of Prunella vulgaris, 0.4 parts by mass of asparagus, 0.6 parts by mass of glutinous rice, 0.5
- Example 16 In addition to the fermentation raw material, 40 parts by mass of corn, 10 parts by mass of wheat, 22 parts by mass of stalked rice, 20 parts by mass of brown rice, 8 parts by mass of rice bran, 38 parts by mass of black beans, 8 parts by mass of black glutinous rice, and 6 parts by mass of black sesame
- the composition, and the raw material in the post-fermentation stage are 60 parts by mass of the preceding fermentation broth, 12 parts by mass of brewer's yeast, 8 parts by mass of Bifidobacterium lactis, 10 parts by mass of calcitonase, 7 parts by mass of oxalic acid decarboxylase, 1 part by mass of melon skin, 5 Quality of Chuanxiong, 3 parts by mass of walnut, 2 parts by weight of phoenix, 1 part by mass of sea sand, 1 mass Cucumber vine, 1 part by mass of pepper, 4 parts of plantain, 2 parts by weight of rose, 2 parts by mass of chayote, 3
- Example 17 In addition to the fermentation raw material, 35 parts by mass of corn, 6 parts by mass of wheat, 10 parts by mass of stalked rice, 9 parts by mass of brown rice, 5 parts by mass of rice bran, 32 parts by mass of black beans, 7 parts by mass of black glutinous rice, and 4 parts by mass of black sesame
- the composition, and the raw material in the post-fermentation stage are 55 parts by mass of the preceding fermentation broth, 6 parts by mass of brewer's yeast, 5 parts by mass of cellulose acetate, 4 parts by mass of lactic acid bacteria, 8 parts by mass of glutamic acid protease, 6 parts by mass of amylase, 4 masses.
- Nattokinase 12 parts by mass of natto, 2 parts by mass of Rhodiola, 1 part by mass of hawthorn, 3 parts by mass of salvia miltiorrhiza, 1 part by mass of pine, 1 part by mass of phytosterol, 3 parts by mass of banana, 1 part by mass Mango, 2 parts by mass of apple, 0.3 parts by mass of lemon, 0.4 parts by mass of kiwi, 0.3 parts by mass of mulberry, 1 part by mass of carrot, 0.8 parts by mass of lettuce, 0.3 parts by mass of amaranth, 0.1 part by mass of cucumber, 0.2 parts by mass of tomato, 0.1 part by mass Sweet potato, 0.2 parts by mass of loofah, 0.1 parts by mass of melon, 0.2 parts by mass of celery, 1 part by mass of shiitake mushrooms, 0.6 parts by mass of Flammulina velutipes, 0.6 parts by mass of black fungus, 0.4 parts by mass of chicken Mushroom, 0.4 parts by mass of the
- Example 18 In addition to the fermentation raw material, 45 parts by mass of corn, 8 parts by mass of wheat, 28 parts by mass of stalked rice, 14 parts by mass of brown rice, 10 parts by mass of rice bran, 32 parts by mass of black beans, 8 parts by mass of black glutinous rice, and 7 parts by mass of black sesame
- the composition and the raw material in the post-fermentation stage are 70 parts by mass of the preceding fermentation broth, 6 parts by mass of Lactobacillus acidophilus, 8 parts by mass of Bifidobacterium, 8 parts by mass of protease, 7 parts by mass of cellulase, 0.1 parts by mass of bamboo shoots, 0.2 mass. ⁇ ⁇ , 0.1 parts by mass of ginger, 0.2 parts by mass of bismuth, 0.7 parts by mass of bismuth, 3 parts by mass of Ganoderma lucidum, 0.1 parts by mass of safflower, 0.2 parts by mass of notoginseng, 2 parts by mass of Chuanxiong, 0.2 parts by mass
- Example 19 In addition to the fermentation raw material, 30 parts by mass of corn, 5 parts by mass of wheat, 15 parts by mass of stalked rice, 5 parts by mass of brown rice, 3 parts by mass of rice bran, 25 parts by mass of black beans, 5 parts by mass of black glutinous rice, and 4 parts by mass of black sesame
- the composition and the raw material in the post-fermentation stage are 45 parts by mass of the pre-fermentation liquid, 7 parts by mass of lactic acid bacteria, 4 parts by mass of Monascus, 5 parts by mass of Saccharomyces cerevisiae, 8 parts by mass of glutamic acid decarboxylase, 2 parts by mass of lysozyme, 3 parts by mass of protease, 5 parts by mass of amylase, 4 parts by mass of lipase, 0.5 parts by mass of citrus, 0.1 parts by mass of ginger, 0.1 parts by mass of ruthenium, 0.7 parts by mass of ruthenium, 3 parts by mass of Ganoderma lucidum,
- Example 20 In addition to the fermentation raw material, 40 parts by mass of corn, 10 parts by mass of wheat, 22 parts by mass of stalked rice, 20 parts by mass of brown rice, 8 parts by mass of rice bran, 38 parts by mass of black beans, 8 parts by mass of black glutinous rice, and 6 parts by mass of black sesame
- the composition, and the raw material in the post-fermentation stage are 60 parts by mass of the preceding fermentation broth, 12 parts by mass of brewer's yeast, 8 parts by mass of Bifidobacterium lactis, 10 parts by mass of calcitonase, 7 parts by mass of oxalic acid decarboxylase, 1 part by mass of melon skin, 5 Quality of Chuanxiong, 3 parts by mass of walnut, 2 parts by mass of phoenix grass, 1 part by mass of sea gold sand, 1 part by mass of cucumber vine, 1 part by mass of pepper, 4 parts of plantain, 2 parts of rose, 2 parts of bergamot melon, 3
- Example 21 In addition to the fermentation raw material, 35 parts by mass of corn, 6 parts by mass of wheat, 10 parts by mass of stalked rice, 9 parts by mass of brown rice, 5 parts by mass of rice bran, 32 parts by mass of black beans, 7 parts by mass of black glutinous rice, and 4 parts by mass of black sesame
- the composition, and the raw material in the post-fermentation stage are 55 parts by mass of the preceding fermentation broth, 6 parts by mass of brewer's yeast, 5 parts by mass of cellulose acetate, 4 parts by mass of lactic acid bacteria, 8 parts by mass of glutamic acid protease, 6 parts by mass of amylase, 4 masses.
- Nattokinase 12 parts by mass of natto, 2 parts by mass of Rhodiola, 1 part by mass of hawthorn, 3 parts by mass of salvia miltiorrhiza, 1 part by mass of pine, 1 part by mass of phytosterol, 3 parts by mass of banana, 1 part by mass Mango, 2 parts by mass of apple, 0.3 parts by mass of lemon, 0.4 parts by mass of kiwi, 0.3 parts by mass of mulberry, 1 part by mass of carrot, 0.8 parts by mass of lettuce, 0.3 parts by mass of amaranth, 0.1 part by mass of cucumber, 0.2 parts by mass of tomato, 0.1 part by mass Sweet potato, 0.2 parts by mass of loofah, 0.1 parts by mass of melon, 0.2 parts by mass of celery, 1 part by mass of shiitake mushrooms, 0.6 parts by mass of Flammulina velutipes, 0.6 parts by mass of black fungus, 0.4 parts by mass of chicken Outer mushroom, 0.4 parts by mass of the
- Example 22 In addition to the fermentation raw material, 45 parts by mass of corn, 8 parts by mass of wheat, 28 parts by mass of stalked rice, 14 parts by mass of brown rice, 10 parts by mass of rice bran, 32 parts by mass of black beans, 8 parts by mass of black glutinous rice, and 7 parts by mass of black sesame
- the composition and the raw material in the post-fermentation stage are 70 parts by mass of the preceding fermentation broth, 6 parts by mass of Lactobacillus acidophilus, 8 parts by mass of Bifidobacterium, 8 parts by mass of protease, 7 parts by mass of cellulase, 0.1 parts by mass of bamboo shoots, 0.2 mass. ⁇ ⁇ , 0.1 parts by mass of ginger, 0.2 parts by mass of bismuth, 0.7 parts by mass of bismuth, 3 parts by mass of Ganoderma lucidum, 0.1 parts by mass of safflower, 0.2 parts by mass of notoginseng, 2 parts by mass of Chuanxiong, 0.2 parts by mass
- Example 23 In addition to the fermentation raw material, 30 parts by mass of corn, 5 parts by mass of wheat, 15 parts by mass of stalked rice, 5 parts by mass of brown rice, 3 parts by mass of rice bran, 25 parts by mass of black beans, 5 parts by mass of black glutinous rice, and 4 parts by mass of black sesame
- the composition and the raw material in the post-fermentation stage are 45 parts by mass of the pre-fermentation liquid, 7 parts by mass of lactic acid bacteria, 4 parts by mass of Monascus, 5 parts by mass of Saccharomyces cerevisiae, 8 parts by mass of glutamic acid decarboxylase, 2 parts by mass of lysozyme, 3 parts by mass of protease, 5 parts by mass of amylase, 4 parts by mass of lipase, 0.5 parts by mass of citrus, 0.1 parts by mass of ginger, 0.1 parts by mass of ruthenium, 0.7 parts by mass of ruthenium, 3 parts by mass of Ganoderma lucidum,
- the enzyme product of the invention can be directly used as a food, and can also be added to other foods and beverages for preventing or assisting in the treatment of various diseases, such as gout enzyme, fossil enzyme, relief enzyme, anti-cancer enzyme, liver-protecting enzyme. , prevention of three high enzymes, gastrointestinal conditioning enzymes, lung enzymes, skin anti-acne enzymes, slimming enzymes, menopausal enzymes, prostate enzymes, antidepressants, anti-aging enzymes, and immune enzymes, have a better overall conditioning effect, and Natural, no toxic side effects. It will be appreciated and appreciated that various modifications and improvements can be made to the present invention described in the Detailed Description without departing from the spirit and scope of the invention. Therefore, the scope of the claimed technical solutions is not limited by any particular exemplary teachings presented.
- the present invention illustrates the detailed process equipment and process flow of the present invention by the above embodiments, but the present invention is not limited to the above detailed process equipment and process flow, that is, it does not mean that the present invention must rely on the above detailed process equipment and The process can only be implemented. It should be apparent to those skilled in the art that any modifications of the present invention, equivalent substitution of the various materials of the products of the present invention, addition of auxiliary components, selection of specific means, and the like, are all within the scope of the present invention.
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Abstract
一种天然调理养生酵素,包含以下以质量计原料组分:1〜80份前发酵液、1〜30份益生菌、1〜30份酶、1〜10份中草药、1〜30份水果、1〜30份蔬菜、1〜30份食用菌、1〜30份食用藻、1〜30份麦芽糖和1〜30份蜂蜜;其中,所述前发酵液由以下以质量计发酵原料1〜50份玉米穗、1〜15份麦、1〜30份梗稻米、1〜50份糙米、1〜50份米糠、1〜50份黑豆、1〜10份黑糯米和1〜10份黑芝麻为主要组成经发酵菌发酵提取制得。
Description
一种天然调理养生酵素及其制造方法
技术领域 本发明涉及酵素的技术领域, 尤其涉及一种天然调理养生酵素及其制造方 法。 背景技术 酵素是酶的产品商品化后的一种说法, 是一种具有催化活性的蛋白质。 无 论动物还是植物, 一切生物的活动都需要酶的参与。 人体不仅每天所摄取食物 的消化吸收、 体内各器官的运作、 细胞的新陈代谢都离不开酶, 而且分解进入 体内的任何物质也需要酶的活动才能够完成。 目前, 随着社会的不断进步, 人 们的健康意识越来越强, 服用纯天然植物酿造的植物酵素来补充人体所必需的 酶是十分有益的。
中草药是中国人民几千年文明历史积累的瑰宝。 酶是维持人的机体生命的 正常功能以及消化食物、 修复组织等生命活动的必需物质。 将药食两用的中草 药与生命必须的酶结合, 将带给人们一种全新的全方位的预防疾病及帮助营养 代谢的理念。 现有技术的酵素难以达到综合调理养生的效果。 发明内容 有鉴于此, 本发明提供一种天然调理养生酵素, 该酵素经科学合理配方, 有效利用天然植物原料接入优良有益菌株进行两步发酵 (指前发酵和后发酵), 并给予充分陈坛, 很好地复合了果蔬、 中草药食材等多种植物的综合调理性, 无毒副作用, 具有较好的综合调理养生效果。
本天然调理养生酵素,包含以下质量份的原料组分: 1〜80份前发酵液、 1〜 30份益生菌、 1〜30份酶、 1〜10份中草药、 1〜30份水果、 1〜30份蔬菜、 1〜
30份食用菌、 1〜30份食用藻、 1〜30份低聚糖和 1〜30份蜂蜜; 其中, 所述前 发酵液由以前发酵原料的质量份数计, 1〜50质量份玉米穗、 1〜15质量份麦、 1〜30质量份梗稻米、 1〜50质量份糙米、 1〜50质量份米糠、 1〜50质量份黑 豆、 1〜10质量份黑糯米和 1〜10质量份黑芝麻为主要组成经发酵菌发酵提取制 得。
作为本发明的优选方案, 所述天然调理养生酵素包含以下质量份的原料组 分: 1〜80份前发酵液、 1〜30份益生菌、 1〜30份酶、 1〜10份中草药、 1〜30 份水果、 1〜30份蔬菜、 1〜30份食用菌、 1〜30份食用藻、 1〜30份麦芽糖和 1〜 30份蜂蜜, 其中, 所述前发酵液为由以前发酵液原料的质量份数计, 1〜50质 量份玉米穗、 1〜15质量份麦、 1〜30质量份梗稻米、 1〜50质量份糙米、 1〜50 质量份米糠、 1〜50质量份黑豆、 1〜10质量份黑糯米和 1〜10质量份黑芝麻为 主要组成经发酵菌发酵制得。
作为本发明的优选方案, 所述酶为糖化酶、 酸性淀粉酶、 ex-淀粉酶、 β-淀粉 酶、 γ-淀粉酶、 异淀粉酶、 脂肪酶、 香蕉酶、 菠萝酶、 木瓜酶、 蛋白酶、 胃蛋白 酶、 弹性酶、 血纤维蛋白溶酶、 结石酶、 植酸酶、 木聚糖酶、 纤维素酶、 半纤 维素酶、 β-葡聚糖酶、草酸脱羧酶、多聚半乳糖醛酸酶、果胶酯酶、果胶水解酶、 乳糖酶、 麦芽糖酶、 β-葡糖苷酶、 花青素酶、 溶菌酶、 尿激酶、 链激酶、 纳豆激 酶、超氧化物歧化酶、过氧化氢酶、辅酶 Α、辅酶 Q-10、透明质酸酶、胶原酶、 壳聚糖水解酶、 乙醇脱氢酶、 乙醛脱氢酶、 三磷酸腺苷酶、 蛋氨酸腺苷酶、 L- 天冬氨酸酶和蔗糖酶中的一种或至少两种。
作为本发明的优选方案, 所述益生菌为啤酒酵母、 膜醋酸菌、 醋酸纤维杆 菌、 乳酸菌、 保加利亚乳酸杆菌、 胚芽乳酸杆菌、 嗜热链球菌、 嗜酸乳杆菌、 比菲德氏菌、 凝结芽孢乳酸菌、 乳双歧杆菌和红曲菌中的一种或至少两种。
作为本发明的优选方案, 所述中草药为虫草、黄精, 灵芝、 人参、 五味子、 莲子、 决明子、 当归、 三七、 何首乌、 石斛、 松花粉、 红景天、 党参、 半支莲、 白花蛇舌草、 仙草、 积雪草、 益母草、 甘草、 小麦草、 牧草、 车前草、 枸杞、
老姜、 川芎、 茯苓、 北柴胡、 熟地黄、 白术、 陈皮、 大枣、 芍药、 竹叶、 枇杷 叶、 川七叶、 荷叶、 桑叶、 黄芪、 海参、 龟甲、 百合、 玉竹、 绞股蓝、 薏苡仁、 益智仁、 葛根、 芦根、 罗汉果、 玫瑰花、 茉莉花、 金银花、 月季花和菊花中的 一种或至少两种。
作为本发明的优选方案, 所述水果为杨梅、 乌梅、 山楂、 柿子、 桃子、 李 子、 椰子、 香瓜、 西瓜、 木瓜、 甜瓜、 哈密瓜、 凤梨、 杨桃、 樱桃、 柠檬、 枇 杷、 甘蔗、 莲雾、 龙眼、 荔枝、 葡萄、 菠萝蜜、 桑葚、 草莓、 柚子、 橙子、 橘 子、 金桔、 番石榴、 火龙果、 香蕉、 芒果、 猕猴桃、 苹果、 枣和佛手瓜中的一 种或至少两种; 所述蔬菜为马铃薯、 胡萝卜、 白萝卜、 白菜、 豆芽、 马齿苋、 山药、 莲藕、 牛蒡、 地瓜、 丝瓜、 苦瓜、 冬瓜、 芹菜、 秋葵、 茼蒿、 菖头、 空 心菜、 韭菜、 上海青、 芥蓝、 苦菜、 椰菜、 龙须菜、 茄子、 青椒、 芦笋、 西红 柿、 黄瓜、 菠菜、 荸荠、 莴苣中的一种或至少两种; 所述食用菌为香菇、 金针 菇、 平菇、 草菇、 鸡腿菇、 木耳和银耳中的一种或至少两种; 所述食用藻为螺 旋藻、 蓝藻、 绿藻、 小球藻、 海带、 紫菜和青海苔中的一种或至少两种。
以上酵素的原料组分中, 前发酵液是指由玉米穗、 麦、 梗稻米、 糙米、 米 糠、 黑豆、 黑糯米和黑芝麻等为原料在含水的环境下经发酵菌的发酵作用得到 的发酵醪经提取后的液体成分。 值得说明的是, 本领域人员所熟知的任何一种 发酵菌均可实施本方案, 例如酵母菌、 黑曲霉菌或米曲霉菌等。 至于水的加入 量, 以不影响发酵菌的繁殖和代谢为原则可通过简单的试验或公知技术获得。 适用于本方案配方之水的加入量可以为 50〜96质量份。
本发明另一方面提供一种制造上述酵素的方法, 该方法得到的酵素具有综 合调理养生的效果。
一种制造如上述酵素的方法, 包括以下步骤:
( 1 )向包含 1〜50质量份玉米穗、 1〜15质量份麦、 1〜30质量份梗稻米、 1〜50质量份糙米、 1〜50质量份米糠、 1〜50质量份黑豆、 1〜10质量份黑糯 米和 1〜10质量份黑芝麻的原料中接种发酵菌并充分混合后, 使其充分发酵经
提取获得前发酵液;
(2)将 1〜80质量份前发酵液连同 1〜30质量份益生菌、 1〜30质量份酶、 1〜10质量份中草药、 1〜30质量份水果、 1〜30质量份蔬菜、 1〜30质量份食 用菌、 1〜30质量份食用藻、 1〜30质量份低聚糖和 1〜30质量份蜂蜜充分混合 后, 使其充分发酵获得后发酵液;
(3 ) 将后发酵液充分陈坛取上清液得到酵素。
作为本发明的优选方案, 所述发酵菌为酵母菌和黑曲霉菌或米曲霉菌, 所 述发酵菌的接种量为 5〜35%; 酵母菌先接种到 250ml三角瓶进行培养, 而后接 种转入 500ml三角瓶进行培养, 再移入发酵容器内; 黑曲霉菌或米曲霉菌菌种 接种到 300ml三角瓶进行培养, 而后接种转入 1000ml三角瓶进行培养, 再移入 发酵容器内; 每个阶段的培养之前要于 O.lMpa压力下蒸汽灭菌 20〜30min, 培 养的条件为: 25〜33 °0恒温培养721。
作为本发明的优选方案, 步骤 (1 ) 之前先后包括将制造前发酵液的原料包 括用水浸渍和蒸料,所述用水浸渍所用水的温度为 25〜30°C,水的 pH为 7〜8, 水硬度为 2〜6mmol/L, 水中铁离子含量不超过 0.5mg/L, 浸渍的时间为 24〜72 小时; 所述蒸料为常压蒸 15〜60min。
作为本发明的优选方案, 步骤 (1 ) 所述发酵的温度为 23〜38°C, 时间为 30〜60天; 发酵过程中要搅拌, 具体的操作方式为: 开始发酵时的温度控制在 23〜30°C,发酵 10小时后进行第一次搅拌,第一次搅拌后发酵的温度控制在 37〜 38°C ;经过 4〜6小时后再进行第二次搅拌,第二次搅拌后发酵的温度控制在 31〜 33 °C ; 又经过 3〜4小时后再进行第三次搅拌, 第三次搅拌后发酵的温度控制在 29〜31 °C ; 又经过 3〜4小时后再进行第四次搅拌, 第四次搅拌后发酵的温度控 制在 27〜30°C ; 此后每天早晚各搅拌一次, 在发酵过程中, 控制温度逐步下降 到 23〜30°C ; 搅拌的时间以达到使发酵料充分均匀; 步骤(2)所述发酵的温度 为 10〜30°C,发酵的时间为 20〜40天;步骤(3 )所述陈坛的温度为 10〜30°C, 陈坛的时间为 80〜600天。
作为本发明的优选方案, 步骤 (3 ) 之后还包括消毒, 所述消毒在蛇管式热 交换器或巴斯灭菌罐中进行,消毒的温度为 75〜90°C,消毒的时间为 4〜10min。
以上酵素的制造方法中, 发酵菌的接种量指移入种子液的体积和接种后培 养基体积的比例。 发酵菌接种到发酵容器之前可以分多个阶段进行菌种培养。 至于以上发酵菌培养所需的营养液属于本领域的公知常识, 在此不再赘述。
本发明的酵素将蔬菜、 水果、 食用菌、 食用藻和中药材与酶组合, 经过有 益菌发酵经提取而得到。 复配中药材可以增强调理的综合性; 复配为维持机体 正常必须的酶和复配纯天然的果蔬菌藻, 无毒副作用。 本发明的酵素采用酶与 植物通过有益菌发酵, 赋予了平凡植物以不平凡的功能, 可直接食用, 也可添 加至饮料、 食品中, 达到了向人们提供其天然综合调理的保健养生效果。 具体实鮮式
下面分别结合实施例对本发明作进一步的详细说明。
实施例 1 : 将由 2质量份玉米、 1质量份麦、 3质量份梗稻米、 1质量份糙 米、 1质量份米糠、 2质量份黑豆、 2质量份黑糯米和 1质量份黑芝麻组成的发 酵原料置入容器中, 加入 50〜96质量份水, 充分混合后在 25〜30°C的温度下浸 渍 24〜72小时, 保证水分吸收量达到 25〜30%。 浸渍所用水的 PH值为 7〜8, 硬度为 2〜6mmol/L, 铁离子含量不超过 0.5mg/L, 且外观清澈透明、 无色、 无 异味。将浸渍后的发酵原料置入蒸料机进行蒸料, 在常压下蒸料 15〜30min, 在 密闭条件下迅速冷却后获得发酵熟料。
前发酵阶段: 将发酵熟料投入大罐, 将先后经 250ml三角瓶和 500ml三角 瓶两次培养的酵母菌菌种按接种量 5〜35%投入至大罐,将先后经 300ml三角瓶 和 1000ml三角瓶两次培养的黑曲霉菌或米曲霉菌菌种按接种量 5〜35%投入大 罐, 将酵母菌菌种、 黑曲霉菌或米曲霉菌菌种和发酵熟料混合、 搅拌均匀, 进 入前发酵的阶段。发酵启动时的温度控制在 23〜30°C, 发酵 10小时后进行第一 次搅拌, 第一次搅拌后发酵的温度控制在 37〜38°C ; 经过 4〜6小时后再进行第
二次搅拌, 第二次搅拌后发酵的温度控制在 31〜33 °C ; 又经过 3〜4小时后再进 行第三次搅拌, 第三次搅拌后发酵的温度控制在 29〜31 °C ; 又经过 3〜4小时后 再进行第四次搅拌, 第四次搅拌后发酵的温度控制在 27〜30°C ; 此后每天早晚 各搅拌一次, 在发酵过程中, 控制温度逐步下降到 23〜30°C。 当经过 30天的发 酵后获得发酵醪。 将发酵醪经提取, 得到前发酵液。
后发酵阶段: 按照以下质量份配比后发酵原料, 即 1质量份前发酵液、 0.1 质量份保加利亚乳酸杆菌、 0.2质量份胚芽乳酸杆菌、 0.2质量份噬热多糖链球 菌、 0.3质量份酵母菌、 0.1质量份嗜酸乳杆菌、 0.5质量份雙歧桿菌、 0.6质量 份超氧化物歧化酶、 0.15 质量份溶菌酶、 0.1 质量份蛋白酶、 0.15 质量份辅酶 Q-10、 0.1质量份石斛、 0.2质量份川芎、 0.1质量份茯苓、 0.1质量份白花蛇舌 草、 0.1质量份半枝蓮、 0.04质量份仙草、 0.06质量份金银花、 0.08质量份大黄、 0.02质量份车前草、 0.2质量份泽泻、 0.2质量份川牛膝、 0.2质量份防己、 0.1 质量份柠檬、 0.1质量份菠萝、 0.2质量份葡萄、 0.1质量份番茄、 0.1质量份梅 子、 0.1质量份苹果、 0.1质量份青木瓜、 0.1质量份哈密瓜、 0.02质量份山药、 0.03质量份牛蒡、 0.03质量份地瓜、 0.05质量份马铃薯、 0.04质量份南瓜、 0.03 质量份红萝卜、 0.02质量份龙葵、 0.04质量份茄子、 0.01 质量份小黄瓜、 0.02 质量份冬瓜、 0.02质量份胡萝卜、 0.02质量份秋葵、 0.01质量份青花椰菜、 0.01 质量份空心菜、 0.03质量份西芹、 0.1质量份大白菜、 0.01质量份菠菜、 0.05质 量份芥蓝、 0.05质量份苦瓜、 0.06质量份小白菜、 0.02质量份上海青、 0.08质 量份甜椒、 0.06质量份青椒、 0.3质量份黄豆芽、 0.05质量份赤芝、 0.05质量份 紫芝、 0.07质量份松杉灵芝、 0.03质量份香菇、 0.04质量份质量份金针菇、 0.1 质量份黑木耳、 0.2质量份平菇、 质量份鸡腿菇、 质量份白木耳、 0.3 质量份虫 草、 0.02质量份质量蓝藻、 0.04质量份海藻、 1份质量麦芽糖和 1质量份蜂蜜进 行充分搅拌混合。 然后, 将上述原料投入大缸进行后发酵的阶段, 并在大缸内 加入 100g/L加入偏重亚硫酸钾防治杂菌污染。 发酵的温度为 10〜30°C, 发酵时 间为 25天以上, 发酵醪经提取获得后发酵液。
将后发酵液装入坛中或大缸中, 至少陈放 80天以上, 陈坛温度 10〜30°C。 将陈坛后的上清发酵液送入蛇管式热交换器或巴斯灭菌罐中低温杀菌; 杀菌的 温度控制在 75〜90°C, 杀菌时间 4〜10min; 消毒后立即在无菌条件下装瓶、 封 口, 获得酵素产品。
实施例 2:除了发酵原料由 50质量份玉米、 15质量份麦、 30质量份梗稻米、 15质量份糙米、 15质量份米糠、 50质量份黑豆、 10质量份黑糯米和 10质量份 黑芝麻组成, 以及后发酵阶段的原料由 80质量份前发酵液、 6质量份凝结芽孢 乳酸菌、 3质量份比菲德氏菌、 4质量份嗜酸乳杆菌、 7质量份双歧杆菌、 4质 量份淀粉酶、 5质量份脂肪酶、 6质量份蛋白酶、 15质量份纤维素酶、 4质量份 川芎、 4质量份仙草、 6质量份金银花、 8质量份大黄、 3质量份车前草、 5质量 份川牛膝、 1质量份柠檬、 2质量份菠萝、 2质量份葡萄、 3质量份苹果、 2质量 份哈密瓜、 1质量份牛蒡、 1质量份马铃薯、 1质量份南瓜、 1质量份茄子、 0.5 质量份冬瓜、 2质量份胡萝卜、 0.5质量份青花椰菜、 1质量份西芹、 0.2质量份 菠菜、 0.3质量份芥蓝、 0.3质量份苦瓜、 0.2质量份青椒、 1 质量份黄豆芽、 3 质量份香菇、 2质量份质量份金针菇、 1质量份黑木耳、 1质量份平菇、 1质量 份鸡腿菇、 1质量份白木耳、 1质量份虫草、 4质量份质量蓝藻、 6质量份紫菜、 30份质量麦芽糖和 30质量份蜂蜜组成外, 其他条件同实施例 1。
实施例 3 :除了发酵原料由 40质量份玉米、 7质量份麦、 15质量份梗稻米、 8质量份糙米、 7质量份米糠、 25质量份黑豆、 6质量份黑糯米和 5质量份黑芝 麻组成, 以及后发酵阶段的原料由 40质量份前发酵液 2质量份保加利亚乳酸杆 菌、 2质量份嗜热链球菌、 1质量份短双歧杆菌、 1质量份比菲德氏菌、 1质量 份过氧化氢酶、 3质量份脂肪酶、 4质量份蛋白酶、 6质量份纤维素酶、 1质量 份超氧化物歧化酶、 6质量份小麦草、 3质量份甜叶菊、 2质量份大麦芽、 4质 量份枸杞、 2质量份葡萄、 1质量份金桔、 0.8质量份甜橙、 0.6质量份柑橘、 0.4 质量份沙田柚、 0.2质量份青木瓜、 0.3 质量份长豆角、 0.2质量份四季豆、 0.2 质量份丝瓜、 0.2质量份小黄瓜、 0.1质量份冬瓜、 0.1质量份苦瓜、 0.4质量份
南瓜、 0.3质量份地瓜叶、 0.2质量份牛蒡、 0.6质量份山药、 0.4质量份莴苣、 0.7质量份西红柿、 0.2质量份茄子、 0.1质量份青椒、 0.2质量份胡萝卜、 0.2质 量份上海青、 0.1质量份苋菜、 0.1质量份空心菜、 0.1质量份大白菜、 0.1质量 份包菜、 0.1质量份菠菜、 0.1质量份芥菜、 2质量份香菇、 0.6质量份质量份金 针菇、 1质量份黑木耳、 0.4质量份鸡腿菇、 0.2质量份虫草、 0.1质量份质量蓝 藻、 0.3质量份紫菜、 0.4质量份海带、 15份质量麦芽糖和 15质量份蜂蜜组成外, 其他条件同实施例 1。
实施例 4: 除了发酵原料由 20质量份玉米、 3质量份麦、 8质量份梗稻米、 4质量份糙米、 3质量份米糠、 12质量份黑豆、 3质量份黑糯米和 2质量份黑芝 麻组成, 以及后发酵阶段的原料由 10质量份前发酵液、 3质量份乳双歧杆菌、 2 质量份啤酒酵母、 0.5质量份嗜热链球菌、 0.5质量份比菲德氏菌、 1质量份大豆 胚芽异黄酮酵素、 0.6质量份脂肪酶、 2质量份蛋白酶、 3质量份纤维素酶、 0.4 质量份辅酶 Q10、 0.5质量份益母草、 0.3质量份百合、 0.2质量份月季花、 0.6 质量份菊花、 0.3质量份玫瑰、 0.2质量份茉莉花、 0.2质量份牡丹皮、 0.2质量 份枇杷叶、 0.4质量份当归、 0.2质量份核桃、 0.5质量份莲子、 0.3质量份枸杞、 0.1质量份玉竹、 0.3质量份黄精、 0.6质量份山茱萸、 0.8质量份茯苓、 0.3质量 份橘皮、 0.5质量份白果芦荟、 0.2质量份黄芪、 0.1质量份黄精、 0.1质量份生 姜、 0.1质量份白术、 1质量份太子参、 0.6质量份白芍、 0.5质量份香蕉、 0.2质 量份芒果、 0.6质量份苹果、 0.1质量份柠檬、 1质量份猕猴桃、 0.3质量份桑葚、 0.6胡萝卜、 0.2莴苣、 0.1苋菜、 0.1黄瓜、 0.2西红柿、 0.1地瓜、 0.1丝瓜、 0.1 冬瓜、 0.3芹菜、 0.2椰菜、 1质量份香菇、 0.4质量份质量份金针菇、 0.3质量份 黑木耳、 0.3质量份鸡腿菇、 0.4质量份质量蓝藻、 0.3质量份紫菜、 0.3质量份 海带、 6份质量麦芽糖和 5质量份蜂蜜组成外, 其他条件同实施例 1。
实施例 5: 除了发酵原料由 30质量份玉米、 4质量份麦、 7质量份梗稻米、 3质量份糙米、 2质量份米糠、 20质量份黑豆、 5质量份黑糯米和 3质量份黑芝 麻组成, 以及后发酵阶段的原料由 15质量份前发酵液、 2质量份啤酒酵母、 0.1
质量份嗜酸乳杆菌、 0.2质量份噬热多糖链球菌、 0.7质量份红曲菌、 0.4质量份 比菲德氏菌、 0.6质量份双歧桿菌、 0.5质量份脂肪酶、 1质量份蛋白酶、 0.2质 量份迷迭香、 0.4质量份芦荟、 0.4质量份齐墩果、 0.3质量份五味子、 0.4质量 份黄芩、 0.6质量份苦参、 0.7质量份当归、 0.4质量份紫苏、 0.2质量份菖蒲、 0.2质量份荷叶、 0.2质量份玫瑰花、 0.2质量份茉莉花、 0.3质量份川芎、 0.3质 量份苍术、 0.5质量份茯苓、 0.2质量份泽泻、 0.2质量份车前草、 0.3质量份夏 枯草、 0.4质量份芦笋、 0.6质量份枇杷、 0.5质量份山楂、 0.5质量份黄柏、 质 量份薏苡、 1质量份香蕉、 0.2质量份芒果、 1质量份苹果、 0.2质量份柠檬、 0.6 质量份猕猴桃、 0.4质量份桑葚、 0.6质量份胡萝卜、 0.1质量份辣椒、 0.1质量 份黄瓜、 0.2质量份西红柿、 0.5质量份洋葱、 0.3质量份芹菜、 0.2质量份椰菜、 1质量份香菇、 0.3质量份质量份金针菇、 0.3质量份平菇、 0.4质量份黑木耳、 0.8质量份质量蓝藻、 0.7质量份紫菜、 1质量份海带、 8份质量麦芽糖和 9质量 份蜂蜜组成外, 其他条件同实施例 1。
实施例 6:除了发酵原料由 40质量份玉米、 10质量份麦、 22质量份梗稻米、 20质量份糙米、 8质量份米糠、 38质量份黑豆、 8质量份黑糯米和 6质量份黑 芝麻组成, 以及后发酵阶段的原料由 60质量份前发酵液、 12质量份啤酒酵母、 8质量份乳双歧杆菌、 10质量份结石酶、 7质量份草酸脱羧酶、 1质量份冬瓜皮、 5质量份川芎、 3质量份核桃、 2质量份凤尾草、 1质量份海金沙、 1质量份黄瓜 藤、 1质量份花椒、 4质量份车前草、 2质量份玫瑰花、 2质量份佛手瓜、 3质量 份香蕉、 2质量份芒果、 4质量份苹果、 1质量份柠檬、 1质量份猕猴桃、 3质量 份胡萝卜、 0.4质量份莴苣、 0.3质量份苋菜、 0.3质量份黄瓜、 2质量份西红柿、 0.3质量份丝瓜、 0.3质量份南瓜、 0.7质量份芹菜、 0.2质量份椰菜、 1质量份香 菇、 0.4质量份质量份金针菇、 0.3质量份黑木耳、 0.3质量份鸡腿菇、 0.4质量 份质量蓝藻、 0.3质量份紫菜、 0.3质量份海带、 6份质量麦芽糖和 5质量份蜂蜜 组成外, 其他条件同实施例 1。
实施例 7:除了发酵原料由 35质量份玉米、 6质量份麦、 10质量份梗稻米、 9质量份糙米、 5质量份米糠、 32质量份黑豆、 7质量份黑糯米和 4质量份黑芝 麻组成, 以及后发酵阶段的原料由 55 质量份前发酵液、 6质量份啤酒酵母、 5 质量份醋酸纤维杆菌、 4质量份乳酸菌、 8质量份谷氨酸蛋白酶、 6质量份淀粉 酶、 4质量份纳豆激酶、 12质量份纳豆、 2质量份红景天、 1质量份山楂、 3质 量份丹参、 1质量份五叶松、 1质量份植物固醇、 3质量份香蕉、 1质量份芒果、 2质量份苹果、 0.3质量份柠檬、 0.4质量份猕猴桃、 0.3质量份桑葚、 1质量份 胡萝卜、 0.8质量份莴苣、 0.3质量份苋菜、 0.1质量份黄瓜、 0.2质量份西红柿、 0.1质量份地瓜、 0.2质量份丝瓜、 0.1质量份冬瓜、 0.2质量份芹菜、 1质量份香 菇、 0.6质量份质量份金针菇、 0.6质量份黑木耳、 0.4质量份鸡腿菇、 0.4质量 份质量蓝藻、 0.3质量份紫菜、 0.3质量份海带、 16份质量麦芽糖和 20质量份蜂 蜜组成外, 其他条件同实施例 1。
实施例 8:除了发酵原料由 45质量份玉米、 8质量份麦、 28质量份梗稻米、 14质量份糙米、 10质量份米糠、 32质量份黑豆、 8质量份黑糯米和 7质量份黑 芝麻组成,以及后发酵阶段的原料由 70质量份前发酵液、 6质量份嗜酸乳杆菌、 8质量份双歧杆菌、 8质量份蛋白酶、 7质量份纤维素酶、 0.1质量份竹笋、 0.2 质量份芡实、 0.1质量份生姜、 0.2质量份薏苡、 0.7质量份枸杞、 3质量份灵芝、 0.1质量份红花、 0.2质量份三七、 2质量份川芎、 0.2质量份黄精、 1质量份黄 芪、 0.3质量份仙草、 0.3质量份牛膝、 0.6质量份当归、 1质量份香蕉、 1质量 份芒果、 3质量份苹果、 0.3质量份柠檬、 0.6质量份猕猴桃、 0.1质量份桑葚、 1 质量份胡萝卜、 0.3质量份莴苣、 0.2质量份苋菜、 0.6质量份西红柿、 0.4质量 份丝瓜、 0.3质量份冬瓜、 2质量份芹菜、 0.2质量份椰菜、 2质量份香菇、 0.6 质量份质量份金针菇、 1质量份黑木耳、 0.4质量份平菇、 0.5质量份质量蓝藻、 1.5质量份紫菜、 1质量份海带、 8份质量麦芽糖和 9质量份蜂蜜组成外, 其他 条件同实施例 1。
实施例 9:除了发酵原料由 30质量份玉米、 5质量份麦、 15质量份梗稻米、 5质量份糙米、 3质量份米糠、 25质量份黑豆、 5质量份黑糯米和 4质量份黑芝 麻组成, 以及后发酵阶段的原料由 45质量份前发酵液、 7质量份乳酸菌、 4质 量份红曲霉菌、 5质量份啤酒酵母菌、 8质量份谷氨酸脱羧酶、 2质量份溶菌酶、 3质量份蛋白酶、 5质量份淀粉酶、 4质量份脂肪酶、 0.5质量份芡实、 0.1质量 份生姜、 0.1 质量份薏苡、 0.7质量份枸杞、 3质量份灵芝、 0.3质量份三七、 2 质量份川芎、 0.4质量份黄精、 1质量份黄芪、 0.3质量份仙草、 0.3质量份牛膝、 0.8质量份当归、 0.5质量份香蕉、 1.5质量份芒果、 3质量份苹果、 0.2质量份柠 檬、 1质量份猕猴桃、 0.3质量份桑葚、 质量份豇豆、 1.5质量份红薯、 质量份粟 米、 1质量份土豆、 0.5质量份山药、 1质量份西红柿、 0.7质量份南瓜、 质量份 0.3黄豆芽、 0.4质量份菠菜、 0.3质量份甘蓝、 1质量份香菇、 0.4质量份质量份 金针菇、 0.3质量份黑木耳、 0.3质量份鸡腿菇、 0.4质量份蓝藻、 0.3质量份紫 菜、 0.3质量份海带、 5质量份麦芽糖和 6质量份蜂蜜组成外, 其他条件同实施 实施例 10: 除了发酵原料由 50质量份玉米、 15质量份麦、 30质量份梗稻 米、 15质量份糙米、 15质量份米糠、 50质量份黑豆、 10质量份黑糯米和 10质 量份黑芝麻组成, 以及后发酵阶段的原料由 80质量份前发酵液、 6质量份凝结 芽孢乳酸菌、 3质量份比菲德氏菌、 4质量份嗜酸乳杆菌、 7质量份双歧杆菌、 4 质量份淀粉酶、 5质量份脂肪酶、 6质量份蛋白酶、 15质量份纤维素酶、 4质量 份川芎、 4质量份仙草、 6质量份金银花、 8质量份大黄、 3质量份车前草、 5质 量份川牛膝、 1质量份柠檬、 2质量份菠萝、 2质量份葡萄、 3质量份苹果、 2质 量份哈密瓜、 1质量份牛蒡、 1质量份马铃薯、 1质量份南瓜、 1质量份茄子、 0.5质量份冬瓜、 2质量份胡萝卜、 0.5质量份青花椰菜、 1质量份西芹、 0.2质 量份菠菜、 0.3质量份芥蓝、 0.3质量份苦瓜、 0.2质量份青椒、 1质量份黄豆芽、 3质量份香菇、 2质量份质量份金针菇、 1质量份黑木耳、 1质量份平菇、 1质量
份鸡腿菇、 1质量份白木耳、 1质量份虫草、 4质量份质量蓝藻、 6质量份紫菜、 30份质量异麦芽低聚糖和 30质量份蜂蜜组成外, 其他条件同实施例 1。
实施例 11 : 除了发酵原料由 50质量份玉米、 15质量份麦、 30质量份梗稻 米、 15质量份糙米、 15质量份米糠、 50质量份黑豆、 10质量份黑糯米和 10质 量份黑芝麻组成, 以及后发酵阶段的原料由 80质量份前发酵液、 6质量份凝结 芽孢乳酸菌、 3质量份比菲德氏菌、 4质量份嗜酸乳杆菌、 7质量份双歧杆菌、 4 质量份淀粉酶、 5质量份脂肪酶、 6质量份蛋白酶、 15质量份纤维素酶、 4质量 份川芎、 4质量份仙草、 6质量份金银花、 8质量份大黄、 3质量份车前草、 5质 量份川牛膝、 1质量份柠檬、 2质量份菠萝、 2质量份葡萄、 3质量份苹果、 2质 量份哈密瓜、 1质量份牛蒡、 1质量份马铃薯、 1质量份南瓜、 1质量份茄子、 0.5质量份冬瓜、 2质量份胡萝卜、 0.5质量份青花椰菜、 1质量份西芹、 0.2质 量份菠菜、 0.3质量份芥蓝、 0.3质量份苦瓜、 0.2质量份青椒、 1质量份黄豆芽、 3质量份香菇、 2质量份质量份金针菇、 1质量份黑木耳、 1质量份平菇、 1质量 份鸡腿菇、 1质量份白木耳、 1质量份虫草、 4质量份质量蓝藻、 6质量份紫菜、 30份质量环状糊精和 30质量份蜂蜜组成外, 其他条件同实施例 1。
实施例 12: 除了发酵原料由 50质量份玉米、 15质量份麦、 30质量份梗稻 米、 15质量份糙米、 15质量份米糠、 50质量份黑豆、 10质量份黑糯米和 10质 量份黑芝麻组成, 以及后发酵阶段的原料由 80质量份前发酵液、 6质量份凝结 芽孢乳酸菌、 3质量份比菲德氏菌、 4质量份嗜酸乳杆菌、 7质量份双歧杆菌、 4 质量份淀粉酶、 5质量份脂肪酶、 6质量份蛋白酶、 15质量份纤维素酶、 4质量 份川芎、 4质量份仙草、 6质量份金银花、 8质量份大黄、 3质量份车前草、 5质 量份川牛膝、 1质量份柠檬、 2质量份菠萝、 2质量份葡萄、 3质量份苹果、 2质 量份哈密瓜、 1质量份牛蒡、 1质量份马铃薯、 1质量份南瓜、 1质量份茄子、 0.5质量份冬瓜、 2质量份胡萝卜、 0.5质量份青花椰菜、 1质量份西芹、 0.2质 量份菠菜、 0.3质量份芥蓝、 0.3质量份苦瓜、 0.2质量份青椒、 1质量份黄豆芽、 3质量份香菇、 2质量份质量份金针菇、 1质量份黑木耳、 1质量份平菇、 1质量
份鸡腿菇、 1质量份白木耳、 1质量份虫草、 4质量份质量蓝藻、 6质量份紫菜、 30份质量龙胆二糖和 30质量份蜂蜜组成外, 其他条件同实施例 1。
实施例 13 : 除了发酵原料由 50质量份玉米、 15质量份麦、 30质量份梗稻 米、 15质量份糙米、 15质量份米糠、 50质量份黑豆、 10质量份黑糯米和 10质 量份黑芝麻组成, 以及后发酵阶段的原料由 80质量份前发酵液、 6质量份凝结 芽孢乳酸菌、 3质量份比菲德氏菌、 4质量份嗜酸乳杆菌、 7质量份双歧杆菌、 4 质量份淀粉酶、 5质量份脂肪酶、 6质量份蛋白酶、 15质量份纤维素酶、 4质量 份川芎、 4质量份仙草、 6质量份金银花、 8质量份大黄、 3质量份车前草、 5质 量份川牛膝、 1质量份柠檬、 2质量份菠萝、 2质量份葡萄、 3质量份苹果、 2质 量份哈密瓜、 1质量份牛蒡、 1质量份马铃薯、 1质量份南瓜、 1质量份茄子、 0.5质量份冬瓜、 2质量份胡萝卜、 0.5质量份青花椰菜、 1质量份西芹、 0.2质 量份菠菜、 0.3质量份芥蓝、 0.3质量份苦瓜、 0.2质量份青椒、 1质量份黄豆芽、 3质量份香菇、 2质量份质量份金针菇、 1质量份黑木耳、 1质量份平菇、 1质量 份鸡腿菇、 1质量份白木耳、 1质量份虫草、 4质量份质量蓝藻、 6质量份紫菜、 30份质量偶联糖和 30质量份蜂蜜组成外, 其他条件同实施例 1。
实施例 14:除了发酵原料由 40质量份玉米、 7质量份麦、 15质量份梗稻米、 8质量份糙米、 7质量份米糠、 25质量份黑豆、 6质量份黑糯米和 5质量份黑芝 麻组成, 以及后发酵阶段的原料由 40质量份前发酵液 2质量份保加利亚乳酸杆 菌、 2质量份嗜热链球菌、 1质量份短双歧杆菌、 1质量份比菲德氏菌、 1质量 份过氧化氢酶、 3质量份脂肪酶、 4质量份蛋白酶、 6质量份纤维素酶、 1质量 份超氧化物歧化酶、 6质量份小麦草、 3质量份甜叶菊、 2质量份大麦芽、 4质 量份枸杞、 2质量份葡萄、 1质量份金桔、 0.8质量份甜橙、 0.6质量份柑橘、 0.4 质量份沙田柚、 0.2质量份青木瓜、 0.3 质量份长豆角、 0.2质量份四季豆、 0.2 质量份丝瓜、 0.2质量份小黄瓜、 0.1质量份冬瓜、 0.1质量份苦瓜、 0.4质量份 南瓜、 0.3质量份地瓜叶、 0.2质量份牛蒡、 0.6质量份山药、 0.4质量份莴苣、 0.7质量份西红柿、 0.2质量份茄子、 0.1质量份青椒、 0.2质量份胡萝卜、 0.2质
量份上海青、 0.1质量份苋菜、 0.1质量份空心菜、 0.1质量份大白菜、 0.1质量 份包菜、 0.1质量份菠菜、 0.1质量份芥菜、 2质量份香菇、 0.6质量份质量份金 针菇、 1质量份黑木耳、 0.4质量份鸡腿菇、 0.2质量份虫草、 0.1质量份质量蓝 藻、 0.3质量份紫菜、 0.4质量份海带、 15份质量果糖低聚糖和 15质量份蜂蜜组 成外, 其他条件同实施例 1。
实施例 15:除了发酵原料由 30质量份玉米、 4质量份麦、 7质量份梗稻米、 3质量份糙米、 2质量份米糠、 20质量份黑豆、 5质量份黑糯米和 3质量份黑芝 麻组成, 以及后发酵阶段的原料由 15质量份前发酵液、 2质量份啤酒酵母、 0.1 质量份嗜酸乳杆菌、 0.2质量份噬热多糖链球菌、 0.7质量份红曲菌、 0.4质量份 比菲德氏菌、 0.6质量份双歧桿菌、 0.5质量份脂肪酶、 1质量份蛋白酶、 0.2质 量份迷迭香、 0.4质量份芦荟、 0.4质量份齐墩果、 0.3质量份五味子、 0.4质量 份黄芩、 0.6质量份苦参、 0.7质量份当归、 0.4质量份紫苏、 0.2质量份菖蒲、 0.2质量份荷叶、 0.2质量份玫瑰花、 0.2质量份茉莉花、 0.3质量份川芎、 0.3质 量份苍术、 0.5质量份茯苓、 0.2质量份泽泻、 0.2质量份车前草、 0.3质量份夏 枯草、 0.4质量份芦笋、 0.6质量份枇杷、 0.5质量份山楂、 0.5质量份黄柏、 质 量份薏苡、 1质量份香蕉、 0.2质量份芒果、 1质量份苹果、 0.2质量份柠檬、 0.6 质量份猕猴桃、 0.4质量份桑葚、 0.6质量份胡萝卜、 0.1质量份辣椒、 0.1质量 份黄瓜、 0.2质量份西红柿、 0.5质量份洋葱、 0.3质量份芹菜、 0.2质量份椰菜、 1质量份香菇、 0.3质量份质量份金针菇、 0.3质量份平菇、 0.4质量份黑木耳、 0.8质量份质量蓝藻、 0.7质量份紫菜、 1质量份海带、 8份质量潘糖和 9质量份 蜂蜜组成外, 其他条件同实施例 1。
实施例 16: 除了发酵原料由 40质量份玉米、 10质量份麦、 22质量份梗稻 米、 20质量份糙米、 8质量份米糠、 38质量份黑豆、 8质量份黑糯米和 6质量 份黑芝麻组成, 以及后发酵阶段的原料由 60质量份前发酵液、 12质量份啤酒酵 母、 8质量份乳双歧杆菌、 10质量份结石酶、 7质量份草酸脱羧酶、 1质量份冬 瓜皮、 5质量份川芎、 3质量份核桃、 2质量份凤尾草、 1质量份海金沙、 1质量
份黄瓜藤、 1质量份花椒、 4质量份车前草、 2质量份玫瑰花、 2质量份佛手瓜、 3质量份香蕉、 2质量份芒果、 4质量份苹果、 1质量份柠檬、 1质量份猕猴桃、 3质量份胡萝卜、 0.4质量份莴苣、 0.3质量份苋菜、 0.3质量份黄瓜、 2质量份 西红柿、 0.3质量份丝瓜、 0.3质量份南瓜、 0.7质量份芹菜、 0.2质量份椰菜、 1 质量份香菇、 0.4质量份质量份金针菇、 0.3质量份黑木耳、 0.3质量份鸡腿菇、 0.4质量份质量蓝藻、 0.3质量份紫菜、 0.3质量份海带、 6份质量海藻糖和 5质 量份蜂蜜组成外, 其他条件同实施例 1。
实施例 17:除了发酵原料由 35质量份玉米、 6质量份麦、 10质量份梗稻米、 9质量份糙米、 5质量份米糠、 32质量份黑豆、 7质量份黑糯米和 4质量份黑芝 麻组成, 以及后发酵阶段的原料由 55 质量份前发酵液、 6质量份啤酒酵母、 5 质量份醋酸纤维杆菌、 4质量份乳酸菌、 8质量份谷氨酸蛋白酶、 6质量份淀粉 酶、 4质量份纳豆激酶、 12质量份纳豆、 2质量份红景天、 1质量份山楂、 3质 量份丹参、 1质量份五叶松、 1质量份植物固醇、 3质量份香蕉、 1质量份芒果、 2质量份苹果、 0.3质量份柠檬、 0.4质量份猕猴桃、 0.3质量份桑葚、 1质量份 胡萝卜、 0.8质量份莴苣、 0.3质量份苋菜、 0.1质量份黄瓜、 0.2质量份西红柿、 0.1质量份地瓜、 0.2质量份丝瓜、 0.1质量份冬瓜、 0.2质量份芹菜、 1质量份香 菇、 0.6质量份质量份金针菇、 0.6质量份黑木耳、 0.4质量份鸡腿菇、 0.4质量 份质量蓝藻、 0.3质量份紫菜、 0.3质量份海带、 16份质量蔗糖低聚糖和 20质量 份蜂蜜组成外, 其他条件同实施例 1。
实施例 18:除了发酵原料由 45质量份玉米、 8质量份麦、 28质量份梗稻米、 14质量份糙米、 10质量份米糠、 32质量份黑豆、 8质量份黑糯米和 7质量份黑 芝麻组成,以及后发酵阶段的原料由 70质量份前发酵液、 6质量份嗜酸乳杆菌、 8质量份双歧杆菌、 8质量份蛋白酶、 7质量份纤维素酶、 0.1质量份竹笋、 0.2 质量份芡实、 0.1质量份生姜、 0.2质量份薏苡、 0.7质量份枸杞、 3质量份灵芝、 0.1质量份红花、 0.2质量份三七、 2质量份川芎、 0.2质量份黄精、 1质量份黄 芪、 0.3质量份仙草、 0.3质量份牛膝、 0.6质量份当归、 1质量份香蕉、 1质量
份芒果、 3质量份苹果、 0.3质量份柠檬、 0.6质量份猕猴桃、 0.1质量份桑葚、 1 质量份胡萝卜、 0.3质量份莴苣、 0.2质量份苋菜、 0.6质量份西红柿、 0.4质量 份丝瓜、 0.3质量份冬瓜、 2质量份芹菜、 0.2质量份椰菜、 2质量份香菇、 0.6 质量份质量份金针菇、 1质量份黑木耳、 0.4质量份平菇、 0.5质量份质量蓝藻、 1.5质量份紫菜、 1质量份海带、 8份质量牛乳低聚糖和 9质量份蜂蜜组成外, 其他条件同实施例 1。
实施例 19:除了发酵原料由 30质量份玉米、 5质量份麦、 15质量份梗稻米、 5质量份糙米、 3质量份米糠、 25质量份黑豆、 5质量份黑糯米和 4质量份黑芝 麻组成, 以及后发酵阶段的原料由 45质量份前发酵液、 7质量份乳酸菌、 4质 量份红曲霉菌、 5质量份啤酒酵母菌、 8质量份谷氨酸脱羧酶、 2质量份溶菌酶、 3质量份蛋白酶、 5质量份淀粉酶、 4质量份脂肪酶、 0.5质量份芡实、 0.1质量 份生姜、 0.1 质量份薏苡、 0.7质量份枸杞、 3质量份灵芝、 0.3质量份三七、 2 质量份川芎、 0.4质量份黄精、 1质量份黄芪、 0.3质量份仙草、 0.3质量份牛膝、 0.8质量份当归、 0.5质量份香蕉、 1.5质量份芒果、 3质量份苹果、 0.2质量份柠 檬、 1质量份猕猴桃、 0.3质量份桑葚、 质量份豇豆、 1.5质量份红薯、 质量份粟 米、 1质量份土豆、 0.5质量份山药、 1质量份西红柿、 0.7质量份南瓜、 质量份 0.3黄豆芽、 0.4质量份菠菜、 0.3质量份甘蓝、 1质量份香菇、 0.4质量份质量份 金针菇、 0.3质量份黑木耳、 0.3质量份鸡腿菇、 0.4质量份蓝藻、 0.3质量份紫 菜、 0.3质量份海带、 5质量份壳质低聚糖和 6质量份蜂蜜组成外, 其他条件同 实施例 1。
实施例 20: 除了发酵原料由 40质量份玉米、 10质量份麦、 22质量份梗稻 米、 20质量份糙米、 8质量份米糠、 38质量份黑豆、 8质量份黑糯米和 6质量 份黑芝麻组成, 以及后发酵阶段的原料由 60质量份前发酵液、 12质量份啤酒酵 母、 8质量份乳双歧杆菌、 10质量份结石酶、 7质量份草酸脱羧酶、 1质量份冬 瓜皮、 5质量份川芎、 3质量份核桃、 2质量份凤尾草、 1质量份海金沙、 1质量 份黄瓜藤、 1质量份花椒、 4质量份车前草、 2质量份玫瑰花、 2质量份佛手瓜、
3质量份香蕉、 2质量份芒果、 4质量份苹果、 1质量份柠檬、 1质量份猕猴桃、 3质量份胡萝卜、 0.4质量份莴苣、 0.3质量份苋菜、 0.3质量份黄瓜、 2质量份 西红柿、 0.3质量份丝瓜、 0.3质量份南瓜、 0.7质量份芹菜、 0.2质量份椰菜、 1 质量份香菇、 0.4质量份质量份金针菇、 0.3质量份黑木耳、 0.3质量份鸡腿菇、 0.4质量份质量蓝藻、 0.3质量份紫菜、 0.3质量份海带、 6份质量大豆低聚糖和 5质量份蜂蜜组成外, 其他条件同实施例 1。
实施例 21 :除了发酵原料由 35质量份玉米、 6质量份麦、 10质量份梗稻米、 9质量份糙米、 5质量份米糠、 32质量份黑豆、 7质量份黑糯米和 4质量份黑芝 麻组成, 以及后发酵阶段的原料由 55 质量份前发酵液、 6质量份啤酒酵母、 5 质量份醋酸纤维杆菌、 4质量份乳酸菌、 8质量份谷氨酸蛋白酶、 6质量份淀粉 酶、 4质量份纳豆激酶、 12质量份纳豆、 2质量份红景天、 1质量份山楂、 3质 量份丹参、 1质量份五叶松、 1质量份植物固醇、 3质量份香蕉、 1质量份芒果、 2质量份苹果、 0.3质量份柠檬、 0.4质量份猕猴桃、 0.3质量份桑葚、 1质量份 胡萝卜、 0.8质量份莴苣、 0.3质量份苋菜、 0.1质量份黄瓜、 0.2质量份西红柿、 0.1质量份地瓜、 0.2质量份丝瓜、 0.1质量份冬瓜、 0.2质量份芹菜、 1质量份香 菇、 0.6质量份质量份金针菇、 0.6质量份黑木耳、 0.4质量份鸡腿菇、 0.4质量 份质量蓝藻、 0.3质量份紫菜、 0.3质量份海带、 16份质量半乳糖低聚糖和 20质 量份蜂蜜组成外, 其他条件同实施例 1。
实施例 22:除了发酵原料由 45质量份玉米、 8质量份麦、 28质量份梗稻米、 14质量份糙米、 10质量份米糠、 32质量份黑豆、 8质量份黑糯米和 7质量份黑 芝麻组成,以及后发酵阶段的原料由 70质量份前发酵液、 6质量份嗜酸乳杆菌、 8质量份双歧杆菌、 8质量份蛋白酶、 7质量份纤维素酶、 0.1质量份竹笋、 0.2 质量份芡实、 0.1质量份生姜、 0.2质量份薏苡、 0.7质量份枸杞、 3质量份灵芝、 0.1质量份红花、 0.2质量份三七、 2质量份川芎、 0.2质量份黄精、 1质量份黄 芪、 0.3质量份仙草、 0.3质量份牛膝、 0.6质量份当归、 1质量份香蕉、 1质量 份芒果、 3质量份苹果、 0.3质量份柠檬、 0.6质量份猕猴桃、 0.1质量份桑葚、 1
质量份胡萝卜、 0.3质量份莴苣、 0.2质量份苋菜、 0.6质量份西红柿、 0.4质量 份丝瓜、 0.3质量份冬瓜、 2质量份芹菜、 0.2质量份椰菜、 2质量份香菇、 0.6 质量份质量份金针菇、 1质量份黑木耳、 0.4质量份平菇、 0.5质量份质量蓝藻、 1.5质量份紫菜、 1质量份海带、 8份质量果糖型低聚糖和 9质量份蜂蜜组成外, 其他条件同实施例 1。
实施例 23 :除了发酵原料由 30质量份玉米、 5质量份麦、 15质量份梗稻米、 5质量份糙米、 3质量份米糠、 25质量份黑豆、 5质量份黑糯米和 4质量份黑芝 麻组成, 以及后发酵阶段的原料由 45质量份前发酵液、 7质量份乳酸菌、 4质 量份红曲霉菌、 5质量份啤酒酵母菌、 8质量份谷氨酸脱羧酶、 2质量份溶菌酶、 3质量份蛋白酶、 5质量份淀粉酶、 4质量份脂肪酶、 0.5质量份芡实、 0.1质量 份生姜、 0.1 质量份薏苡、 0.7质量份枸杞、 3质量份灵芝、 0.3质量份三七、 2 质量份川芎、 0.4质量份黄精、 1质量份黄芪、 0.3质量份仙草、 0.3质量份牛膝、 0.8质量份当归、 0.5质量份香蕉、 1.5质量份芒果、 3质量份苹果、 0.2质量份柠 檬、 1质量份猕猴桃、 0.3质量份桑葚、 质量份豇豆、 1.5质量份红薯、 质量份粟 米、 1质量份土豆、 0.5质量份山药、 1质量份西红柿、 0.7质量份南瓜、 质量份 0.3黄豆芽、 0.4质量份菠菜、 0.3质量份甘蓝、 1质量份香菇、 0.4质量份质量份 金针菇、 0.3质量份黑木耳、 0.3质量份鸡腿菇、 0.4质量份蓝藻、 0.3质量份紫 菜、 0.3质量份海带、 5质量份木低聚糖和 6质量份蜂蜜组成外, 其他条件同实 施例 1。
本发明的酵素产品可作为食品直接使用, 也可添加至其它食品和饮料中, 用于预防或辅助治疗多种疾病, 例如痛风酵素、 化石酵素、 安心酵素、 抗癌酵 素、 护肝解酒酵素、预防三高酵素、肠胃调理酵素、清肺酵素、美肤抗痘酵素、 减肥酵素、 更年期酵素、 前列腺酵素、 抗抑郁酵素、 抗衰老酵素、 提高免疫酵 素, 具有较好的综合调理作用, 并且纯天然, 无毒副作用。
应该注意到并理解, 在不脱离后附的权利要求所要求保护的本发明的精神 和范围的情况下, 能够对上述详细描述的本发明做出各种修改和改进。 因此, 要求保护的技术方案的范围不受所给出的任何特定示范教导的限制。
申请人声明, 本发明通过上述实施例来说明本发明的详细工艺设备和工艺 流程, 但本发明并不局限于上述详细工艺设备和工艺流程, 即不意味着本发明 必须依赖上述详细工艺设备和工艺流程才能实施。 所属技术领域的技术人员应 该明了, 对本发明的任何改进, 对本发明产品各原料的等效替换及辅助成分的 添加、 具体方式的选择等, 均落在本发明的保护范围和公开范围之内。
Claims
1.一种天然调理养生酵素,其特征在于,包含以下质量份的原料组分: 1〜 80份前发酵液、 1〜30份益生菌、 1〜30份酶、 1〜10份中草药、 1〜30份水果、 1〜30份蔬菜、 1〜30份食用菌、 1〜30份食用藻、 1〜30份低聚糖和 1〜30份 蜂蜜; 其中, 所述前发酵液由以前发酵原料的质量份数计, 1〜50质量份玉米 穗、 1〜15质量份麦、 1〜30质量份梗稻米、 1〜50质量份糙米、 1〜50质量份 米糠、 1〜50质量份黑豆、 1〜10质量份黑糯米和 1〜10质量份黑芝麻为主要组 成经发酵菌发酵提取制得。
2.根据权利要求 1所述的酵素,其特征在于,包含以下质量份的原料组分: 1〜80份前发酵液、 1〜30份益生菌、 1〜30份酶、 1〜10份中草药、 1〜30份 水果、 1〜30份蔬菜、 1〜30份食用菌、 1〜30份食用藻、 1〜30份麦芽糖和 1〜 30份蜂蜜; 其中, 所述前发酵液由以前发酵原料的质量份数计, 1〜50质量份 玉米穗、 1〜15质量份麦、 1〜30质量份梗稻米、 1〜50质量份糙米、 1〜50质 量份米糠、 1〜50质量份黑豆、 1〜10质量份黑糯米和 1〜10质量份黑芝麻为主 要组成经发酵菌发酵提取制得。
3. 根据权利要求 1或 2所述的酵素, 其特征在于, 所述酶为糖化酶、酸性 淀粉酶、 α-淀粉酶、 β-淀粉酶、 γ-淀粉酶、异淀粉酶、脂肪酶、香蕉酶、菠萝酶、 木瓜酶、 蛋白酶、 胃蛋白酶、 弹性酶、 血纤维蛋白溶酶、 结石酶、 植酸酶、 木 聚糖酶、 纤维素酶、 半纤维素酶、 β-葡聚糖酶、 草酸脱羧酶、 多聚半乳糖醛酸 酶、 果胶酯酶、 果胶水解酶、 乳糖酶、 麦芽糖酶、 β-葡糖苷酶、 花青素酶、 溶 菌酶、 尿激酶、 链激酶、 纳豆激酶、 超氧化物歧化酶、 过氧化氢酶、 辅酶 Α、 辅酶 Q-10、 透明质酸酶、 胶原酶、 壳聚糖水解酶、 乙醇脱氢酶、 乙醛脱氢酶、 三磷酸腺苷酶、 蛋氨酸腺苷酶、 L-天冬氨酸酶和蔗糖酶中的一种或至少两种。
4.根据权利要求 1或 2所述的酵素,其特征在于,所述益生菌为啤酒酵母、 膜醋酸菌、 醋酸纤维杆菌、 乳酸菌、 保加利亚乳酸杆菌、 胚芽乳酸杆菌、 嗜热 链球菌、 嗜酸乳杆菌、 比菲德氏菌、 凝结芽孢乳酸菌、 乳双歧杆菌和红曲菌中 的一种或至少两种。
5. 根据权利要求 1或 2所述的酵素, 其特征在于, 所述中草药为虫草、黄 精, 灵芝、 人参、 五味子、 莲子、 决明子 、 当归、 三七、 何首乌、 石斛、 松花 粉、 红景天、 党参、 半支莲、 白花蛇舌草、 仙草、 积雪草、 益母草、 甘草、 小 麦草、 牧草、 车前草、 枸杞、 老姜、 川芎、 茯苓、 北柴胡、 熟地黄、 白术、 陈 皮、 大枣、 芍药、 竹叶、 枇杷叶、 川七叶、 荷叶、 桑叶、 黄芪、 海参、 龟甲、 百合、 玉竹、 绞股蓝、 薏苡仁、 益智仁、 葛根、 芦根、 罗汉果、 玫瑰花、 茉莉 花、 金银花、 月季花和菊花中的一种或至少两种。
6.根据权利要求 1或 2所述的酵素,其特征在于,所述水果为杨梅、乌梅、 山楂、 柿子、 桃子、 李子、 椰子、 香瓜、 西瓜、 木瓜、 甜瓜、 哈密瓜、 凤梨、 杨桃、 樱桃、 柠檬、 枇杷、 甘蔗、 莲雾、 龙眼、 荔枝、 葡萄、 菠萝蜜、 桑葚、 草莓、 柚子、 橙子、 橘子、 金桔、 番石榴、 火龙果、 香蕉、 芒果、 猕猴桃、 苹 果、 枣和佛手瓜中的一种或至少两种; 所述蔬菜为马铃薯、 胡萝卜、 白萝卜、 白菜、 豆芽、 马齿苋、 山药、 莲藕、 牛蒡、 地瓜、 丝瓜、 苦瓜、 冬瓜、 芹菜、 秋葵、 茼蒿、 菖头、 空心菜、 韭菜、 上海青、 芥蓝、 苦菜、 椰菜、 龙须菜、 茄 子、 青椒、 芦笋、 西红柿、 黄瓜、 菠菜、 荸荠、 莴苣中的一种或至少两种; 所 述食用菌为香菇、 金针菇、 平菇、 草菇、 鸡腿菇、 木耳和银耳中的一种或至少 两种; 所述食用藻为螺旋藻、 蓝藻、 绿藻、 小球藻、 海带、 紫菜和青海苔中的 一种或至少两种。
7. —种制造如权利要求 1-6任一项所述酵素的方法, 其特征在于, 包括以 下步骤:
( 1 )向包含 1〜50质量份玉米穗、 1〜15质量份麦、 1〜30质量份梗稻米、 1〜50质量份糙米、 1〜50质量份米糠、 1〜50质量份黑豆、 1〜10质量份黑糯 米和 1〜10质量份黑芝麻的原料中接种发酵菌并充分混合后, 使其充分发酵经 提取获得前发酵液;
(2) 将 1〜80份前发酵液、 1〜30份益生菌、 1〜30份酶、 1〜10份中草 药、 1〜30份水果、 1〜30份蔬菜、 1〜30份食用菌、 1〜30份食用藻、 1〜30
份低聚糖和 1〜30份蜂蜜充分混合后, 使其充分发酵获得后发酵液; (3 ) 将后发酵液充分陈坛取上清液得到酵素。
8. 根据权利要求 7所述的方法, 其特征在于, 所述发酵菌为酵母菌、 黑曲 霉菌或米曲霉菌, 所述发酵菌的接种量为 5〜35%; 酵母菌先接种到 250ml三 角瓶进行培养, 而后接种转入 500ml三角瓶进行培养, 再移入发酵容器内; 黑 曲霉菌或米曲霉菌菌种接种到 300ml三角瓶进行培养, 而后接种转入 1000ml 三角瓶进行培养, 再移入发酵容器内; 每个阶段的培养之前要于 O.lMpa压力 下蒸汽灭菌 20〜30min, 培养的条件为: 25〜33°C恒温培养 72h。
9. 根据权利要求 7所述的方法, 其特征在于, 步骤 (1 ) 之前包括, 将制 造前发酵液的原料包括用水浸渍和蒸料, 所述用水浸渍所用水的温度为 25〜 30°C,水的 pH为 7〜8,水硬度为 2〜6mmol/L,水中铁离子含量不超过 0.5mg/L, 浸渍的时间为 24〜72小时; 所述蒸料为常压蒸 15〜60min。
10. 根据权利要求 7所述的方法, 其特征在于, 步骤(1 )所述发酵的温度 为 23〜38°C, 时间为 30〜60天; 发酵过程中要搅拌, 具体的操作方式为: 开 始发酵时的温度控制在 23〜30°C, 发酵 10小时后进行第一次搅拌, 第一次搅 拌后发酵的温度控制在 37〜38°C ; 经过 4〜6小时后再进行第二次搅拌, 第二 次搅拌后发酵的温度控制在 31〜33°C;又经过 3〜4小时后再进行第三次搅拌, 第三次搅拌后发酵的温度控制在 29〜31 °C ; 又经过 3〜4小时后再进行第四次 搅拌, 第四次搅拌后发酵的温度控制在 27〜30°C ; 此后每天早晚各搅拌一次, 在发酵过程中, 控制温度逐步下降到 23〜30°C ; 搅拌的时间以达到使发酵料充 分均匀; 步骤 (2)所述发酵的温度为 10〜30°C, 发酵的时间为 20〜40天; 步 骤 (3 ) 所述陈坛的温度为 10〜30°C, 陈坛的时间为 80〜600天。
11.根据权利要求 7所述的方法,其特征在于,步骤(3 )之后还包括消毒, 所述消毒在蛇管式热交换器或巴斯灭菌罐中进行, 消毒的温度为 75〜90°C, 消 毒的时间为 4〜10min。
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