CN106071605A - 一种灵芝发酵制备酵素的方法 - Google Patents
一种灵芝发酵制备酵素的方法 Download PDFInfo
- Publication number
- CN106071605A CN106071605A CN201610521835.0A CN201610521835A CN106071605A CN 106071605 A CN106071605 A CN 106071605A CN 201610521835 A CN201610521835 A CN 201610521835A CN 106071605 A CN106071605 A CN 106071605A
- Authority
- CN
- China
- Prior art keywords
- strain
- ferment
- fermentation
- ganoderma
- glossy ganoderma
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 241000222336 Ganoderma Species 0.000 title claims abstract description 37
- 238000000855 fermentation Methods 0.000 title claims abstract description 28
- 238000000034 method Methods 0.000 title claims abstract description 21
- 230000004151 fermentation Effects 0.000 title claims description 18
- 239000003651 drinking water Substances 0.000 claims abstract description 17
- 235000020188 drinking water Nutrition 0.000 claims abstract description 17
- 239000002994 raw material Substances 0.000 claims abstract description 13
- 235000011194 food seasoning agent Nutrition 0.000 claims abstract description 10
- 240000001046 Lactobacillus acidophilus Species 0.000 claims abstract description 9
- 235000013956 Lactobacillus acidophilus Nutrition 0.000 claims abstract description 9
- 241000218588 Lactobacillus rhamnosus Species 0.000 claims abstract description 9
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims abstract description 6
- 229930006000 Sucrose Natural products 0.000 claims abstract description 6
- 239000004278 EU approved seasoning Substances 0.000 claims abstract description 5
- AYRXSINWFIIFAE-SCLMCMATSA-N Isomaltose Natural products OC[C@H]1O[C@H](OC[C@@H](O)[C@@H](O)[C@H](O)[C@@H](O)C=O)[C@@H](O)[C@@H](O)[C@@H]1O AYRXSINWFIIFAE-SCLMCMATSA-N 0.000 claims abstract description 5
- 244000199866 Lactobacillus casei Species 0.000 claims abstract description 5
- 235000013958 Lactobacillus casei Nutrition 0.000 claims abstract description 5
- DLRVVLDZNNYCBX-RTPHMHGBSA-N isomaltose Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1OC[C@@H]1[C@@H](O)[C@H](O)[C@@H](O)C(O)O1 DLRVVLDZNNYCBX-RTPHMHGBSA-N 0.000 claims abstract description 5
- 229940017800 lactobacillus casei Drugs 0.000 claims abstract description 5
- UOQHWNPVNXSDDO-UHFFFAOYSA-N 3-bromoimidazo[1,2-a]pyridine-6-carbonitrile Chemical compound C1=CC(C#N)=CN2C(Br)=CN=C21 UOQHWNPVNXSDDO-UHFFFAOYSA-N 0.000 claims abstract description 4
- 229940099563 lactobionic acid Drugs 0.000 claims abstract description 4
- 239000001963 growth medium Substances 0.000 claims description 11
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 8
- 238000012258 culturing Methods 0.000 claims description 8
- 238000011010 flushing procedure Methods 0.000 claims description 7
- 238000007654 immersion Methods 0.000 claims description 7
- 238000005406 washing Methods 0.000 claims description 7
- 235000011389 fruit/vegetable juice Nutrition 0.000 claims description 5
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 claims description 4
- 239000000843 powder Substances 0.000 claims description 4
- 235000012907 honey Nutrition 0.000 claims description 3
- 239000000203 mixture Substances 0.000 claims description 3
- 102000004169 proteins and genes Human genes 0.000 claims description 3
- 108090000623 proteins and genes Proteins 0.000 claims description 3
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 2
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims description 2
- VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical compound [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 claims description 2
- 235000015278 beef Nutrition 0.000 claims description 2
- 239000000356 contaminant Substances 0.000 claims description 2
- YXVFQADLFFNVDS-UHFFFAOYSA-N diammonium citrate Chemical compound [NH4+].[NH4+].[O-]C(=O)CC(O)(C(=O)O)CC([O-])=O YXVFQADLFFNVDS-UHFFFAOYSA-N 0.000 claims description 2
- 238000009792 diffusion process Methods 0.000 claims description 2
- 239000008103 glucose Substances 0.000 claims description 2
- 239000007788 liquid Substances 0.000 claims description 2
- 229910052943 magnesium sulfate Inorganic materials 0.000 claims description 2
- 235000019341 magnesium sulphate Nutrition 0.000 claims description 2
- 229940099596 manganese sulfate Drugs 0.000 claims description 2
- 239000011702 manganese sulphate Substances 0.000 claims description 2
- 235000007079 manganese sulphate Nutrition 0.000 claims description 2
- SQQMAOCOWKFBNP-UHFFFAOYSA-L manganese(II) sulfate Chemical compound [Mn+2].[O-]S([O-])(=O)=O SQQMAOCOWKFBNP-UHFFFAOYSA-L 0.000 claims description 2
- 235000010482 polyoxyethylene sorbitan monooleate Nutrition 0.000 claims description 2
- 229920000053 polysorbate 80 Polymers 0.000 claims description 2
- 239000001632 sodium acetate Substances 0.000 claims description 2
- 235000017281 sodium acetate Nutrition 0.000 claims description 2
- 210000004243 sweat Anatomy 0.000 claims description 2
- 241000251468 Actinopterygii Species 0.000 claims 1
- 241001219085 Cyclopia Species 0.000 claims 1
- 235000013616 tea Nutrition 0.000 claims 1
- 108090000790 Enzymes Proteins 0.000 abstract description 10
- 102000004190 Enzymes Human genes 0.000 abstract description 10
- 235000013361 beverage Nutrition 0.000 abstract description 10
- 238000001914 filtration Methods 0.000 abstract description 5
- 238000005728 strengthening Methods 0.000 abstract description 4
- 206010020772 Hypertension Diseases 0.000 abstract description 2
- 239000008280 blood Substances 0.000 abstract description 2
- 210000004369 blood Anatomy 0.000 abstract description 2
- 210000000952 spleen Anatomy 0.000 abstract description 2
- 210000002784 stomach Anatomy 0.000 abstract description 2
- 230000002936 tranquilizing effect Effects 0.000 abstract description 2
- 230000003203 everyday effect Effects 0.000 abstract 1
- 230000000694 effects Effects 0.000 description 10
- 229940088598 enzyme Drugs 0.000 description 9
- 238000002156 mixing Methods 0.000 description 6
- 241000186605 Lactobacillus paracasei Species 0.000 description 4
- 239000000796 flavoring agent Substances 0.000 description 4
- 235000019634 flavors Nutrition 0.000 description 4
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Natural products OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 description 2
- 206010011224 Cough Diseases 0.000 description 2
- 206010003549 asthenia Diseases 0.000 description 2
- 208000006673 asthma Diseases 0.000 description 2
- WPYMKLBDIGXBTP-UHFFFAOYSA-N benzoic acid Chemical compound OC(=O)C1=CC=CC=C1 WPYMKLBDIGXBTP-UHFFFAOYSA-N 0.000 description 2
- 239000003814 drug Substances 0.000 description 2
- 235000013399 edible fruits Nutrition 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 235000018102 proteins Nutrition 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- HDTRYLNUVZCQOY-UHFFFAOYSA-N α-D-glucopyranosyl-α-D-glucopyranoside Natural products OC1C(O)C(O)C(CO)OC1OC1C(O)C(O)C(O)C(CO)O1 HDTRYLNUVZCQOY-UHFFFAOYSA-N 0.000 description 1
- OILXMJHPFNGGTO-UHFFFAOYSA-N (22E)-(24xi)-24-methylcholesta-5,22-dien-3beta-ol Natural products C1C=C2CC(O)CCC2(C)C2C1C1CCC(C(C)C=CC(C)C(C)C)C1(C)CC2 OILXMJHPFNGGTO-UHFFFAOYSA-N 0.000 description 1
- RQOCXCFLRBRBCS-UHFFFAOYSA-N (22E)-cholesta-5,7,22-trien-3beta-ol Natural products C1C(O)CCC2(C)C(CCC3(C(C(C)C=CCC(C)C)CCC33)C)C3=CC=C21 RQOCXCFLRBRBCS-UHFFFAOYSA-N 0.000 description 1
- OQMZNAMGEHIHNN-UHFFFAOYSA-N 7-Dehydrostigmasterol Natural products C1C(O)CCC2(C)C(CCC3(C(C(C)C=CC(CC)C(C)C)CCC33)C)C3=CC=C21 OQMZNAMGEHIHNN-UHFFFAOYSA-N 0.000 description 1
- GUBGYTABKSRVRQ-XLOQQCSPSA-N Alpha-Lactose Chemical compound O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@H]1O[C@@H]1[C@@H](CO)O[C@H](O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-XLOQQCSPSA-N 0.000 description 1
- 206010002383 Angina Pectoris Diseases 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- 239000005711 Benzoic acid Substances 0.000 description 1
- AUNGANRZJHBGPY-UHFFFAOYSA-N D-Lyxoflavin Natural products OCC(O)C(O)C(O)CN1C=2C=C(C)C(C)=CC=2N=C2C1=NC(=O)NC2=O AUNGANRZJHBGPY-UHFFFAOYSA-N 0.000 description 1
- FBPFZTCFMRRESA-KVTDHHQDSA-N D-Mannitol Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-KVTDHHQDSA-N 0.000 description 1
- ZZZCUOFIHGPKAK-UHFFFAOYSA-N D-erythro-ascorbic acid Natural products OCC1OC(=O)C(O)=C1O ZZZCUOFIHGPKAK-UHFFFAOYSA-N 0.000 description 1
- 208000000059 Dyspnea Diseases 0.000 description 1
- 206010013975 Dyspnoeas Diseases 0.000 description 1
- DNVPQKQSNYMLRS-NXVQYWJNSA-N Ergosterol Natural products CC(C)[C@@H](C)C=C[C@H](C)[C@H]1CC[C@H]2C3=CC=C4C[C@@H](O)CC[C@]4(C)[C@@H]3CC[C@]12C DNVPQKQSNYMLRS-NXVQYWJNSA-N 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- 241001149422 Ganoderma applanatum Species 0.000 description 1
- 240000008397 Ganoderma lucidum Species 0.000 description 1
- 235000001637 Ganoderma lucidum Nutrition 0.000 description 1
- 241001480597 Ganoderma tsugae Species 0.000 description 1
- 206010061192 Haemorrhagic fever Diseases 0.000 description 1
- 208000019926 Keshan disease Diseases 0.000 description 1
- GUBGYTABKSRVRQ-QKKXKWKRSA-N Lactose Natural products OC[C@H]1O[C@@H](O[C@H]2[C@H](O)[C@@H](O)C(O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@H]1O GUBGYTABKSRVRQ-QKKXKWKRSA-N 0.000 description 1
- 229930195725 Mannitol Natural products 0.000 description 1
- 102000016943 Muramidase Human genes 0.000 description 1
- 108010014251 Muramidase Proteins 0.000 description 1
- 108010062010 N-Acetylmuramoyl-L-alanine Amidase Proteins 0.000 description 1
- 208000007443 Neurasthenia Diseases 0.000 description 1
- 206010033557 Palpitations Diseases 0.000 description 1
- 238000012356 Product development Methods 0.000 description 1
- AUNGANRZJHBGPY-SCRDCRAPSA-N Riboflavin Chemical compound OC[C@@H](O)[C@@H](O)[C@@H](O)CN1C=2C=C(C)C(C)=CC=2N=C2C1=NC(=O)NC2=O AUNGANRZJHBGPY-SCRDCRAPSA-N 0.000 description 1
- 244000000231 Sesamum indicum Species 0.000 description 1
- 235000003434 Sesamum indicum Nutrition 0.000 description 1
- 208000013738 Sleep Initiation and Maintenance disease Diseases 0.000 description 1
- 235000021355 Stearic acid Nutrition 0.000 description 1
- 206010044302 Tracheitis Diseases 0.000 description 1
- HDTRYLNUVZCQOY-WSWWMNSNSA-N Trehalose Natural products O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-WSWWMNSNSA-N 0.000 description 1
- 229930003471 Vitamin B2 Natural products 0.000 description 1
- 229930003268 Vitamin C Natural products 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 229930013930 alkaloid Natural products 0.000 description 1
- 150000003797 alkaloid derivatives Chemical class 0.000 description 1
- HDTRYLNUVZCQOY-LIZSDCNHSA-N alpha,alpha-trehalose Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-LIZSDCNHSA-N 0.000 description 1
- 235000001014 amino acid Nutrition 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 206010003119 arrhythmia Diseases 0.000 description 1
- 230000006793 arrhythmia Effects 0.000 description 1
- 244000052616 bacterial pathogen Species 0.000 description 1
- 239000000688 bacterial toxin Substances 0.000 description 1
- 235000010233 benzoic acid Nutrition 0.000 description 1
- POIARNZEYGURDG-FNORWQNLSA-N beta-damascenone Chemical compound C\C=C\C(=O)C1=C(C)C=CCC1(C)C POIARNZEYGURDG-FNORWQNLSA-N 0.000 description 1
- 230000000975 bioactive effect Effects 0.000 description 1
- 230000002210 biocatalytic effect Effects 0.000 description 1
- 230000004071 biological effect Effects 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- 229930182483 coumarin glycoside Natural products 0.000 description 1
- 150000008140 coumarin glycosides Chemical class 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 208000002173 dizziness Diseases 0.000 description 1
- 201000006549 dyspepsia Diseases 0.000 description 1
- DNVPQKQSNYMLRS-SOWFXMKYSA-N ergosterol Chemical compound C1[C@@H](O)CC[C@]2(C)[C@H](CC[C@]3([C@H]([C@H](C)/C=C/[C@@H](C)C(C)C)CC[C@H]33)C)C3=CC=C21 DNVPQKQSNYMLRS-SOWFXMKYSA-N 0.000 description 1
- 201000005577 familial hyperlipidemia Diseases 0.000 description 1
- 238000009472 formulation Methods 0.000 description 1
- 235000021433 fructose syrup Nutrition 0.000 description 1
- 230000002538 fungal effect Effects 0.000 description 1
- 150000004676 glycans Chemical class 0.000 description 1
- 230000008821 health effect Effects 0.000 description 1
- 208000006454 hepatitis Diseases 0.000 description 1
- 231100000283 hepatitis Toxicity 0.000 description 1
- 206010022437 insomnia Diseases 0.000 description 1
- 210000003734 kidney Anatomy 0.000 description 1
- 239000008101 lactose Substances 0.000 description 1
- 229960000274 lysozyme Drugs 0.000 description 1
- 235000010335 lysozyme Nutrition 0.000 description 1
- 239000004325 lysozyme Substances 0.000 description 1
- 229920002521 macromolecule Polymers 0.000 description 1
- 239000000594 mannitol Substances 0.000 description 1
- 235000010355 mannitol Nutrition 0.000 description 1
- 239000008204 material by function Substances 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- QIQXTHQIDYTFRH-UHFFFAOYSA-N octadecanoic acid Chemical compound CCCCCCCCCCCCCCCCCC(O)=O QIQXTHQIDYTFRH-UHFFFAOYSA-N 0.000 description 1
- OQCDKBAXFALNLD-UHFFFAOYSA-N octadecanoic acid Natural products CCCCCCCC(C)CCCCCCCCC(O)=O OQCDKBAXFALNLD-UHFFFAOYSA-N 0.000 description 1
- 230000000144 pharmacologic effect Effects 0.000 description 1
- 229920001184 polypeptide Polymers 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 102000004196 processed proteins & peptides Human genes 0.000 description 1
- 108090000765 processed proteins & peptides Proteins 0.000 description 1
- 229960002477 riboflavin Drugs 0.000 description 1
- 208000013220 shortness of breath Diseases 0.000 description 1
- 150000003384 small molecules Chemical class 0.000 description 1
- 239000008117 stearic acid Substances 0.000 description 1
- 239000005720 sucrose Substances 0.000 description 1
- 239000006188 syrup Substances 0.000 description 1
- 235000020357 syrup Nutrition 0.000 description 1
- 235000015961 tonic Nutrition 0.000 description 1
- 230000001256 tonic effect Effects 0.000 description 1
- 229960000716 tonics Drugs 0.000 description 1
- 150000003648 triterpenes Chemical class 0.000 description 1
- 235000019164 vitamin B2 Nutrition 0.000 description 1
- 239000011716 vitamin B2 Substances 0.000 description 1
- 235000019154 vitamin C Nutrition 0.000 description 1
- 239000011718 vitamin C Substances 0.000 description 1
- 239000000341 volatile oil Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
- A23L2/38—Other non-alcoholic beverages
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
- A23L2/70—Clarifying or fining of non-alcoholic beverages; Removing unwanted matter
- A23L2/84—Clarifying or fining of non-alcoholic beverages; Removing unwanted matter using microorganisms or biological material, e.g. enzymes
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/113—Acidophilus
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/125—Casei
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/175—Rhamnosus
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Nutrition Science (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Zoology (AREA)
- Non-Alcoholic Beverages (AREA)
Abstract
本发明提供了一种用市售干酪乳杆菌、鼠李糖乳杆菌，嗜酸乳杆菌发酵制备灵芝酵素饮料的方法，其工艺特点是以灵芝主要原料，辅以蜂蜜和菌种。原料适当打碎后加蜂蜜和菌种各100毫升，饮用水10升。30℃厌氧发酵5‑10天。过滤后可加蜂蜜、低聚果糖、低聚异麦芽糖、白砂糖、乳糖酸等调味。每天早晚各饮用一次，每次50‑100毫升。可益气血、安心神、健脾胃，防治高血压。
Description
技术领域
本发明涉及灵芝酵素的生产方法，尤其是灵芝为原料的酵素保健饮料的发酵方法，属于生物工程技术领域。
背景技术
酵素一词是日本人对酶(enzyme)的一种叫法，指具有生物催化功能的大分子物质，后来把经微生物发酵得到的具有生物活性物质的发酵液或发酵制品统称为酵素。鉴于一些制作者只关注水果，尤其是加入不同种类的水果，不注意发酵过程中微生物的作用，也忽视了对一些功能性材料的应用，有必要应用传统和科学搭配制作酵素产品、拓展和保证酵素功能，为民众健康提供保障。酵素产品的开发和应用还易受到杂菌污染的影响，因此也有必要应用科学的发酵技术以保证产品质量。
灵芝，外形呈伞状，菌盖肾形、半圆形或近圆形，为多孔菌科真菌灵芝的子实体。具有补气安神、止咳平喘的功效，用于眩晕不眠、心悸气短、虚劳咳喘。灵芝已实现人工种植。灵芝药用在我国已有2000多年的历史，被历代医药家视为滋补强壮、扶正固本的神奇珍品。已知灵芝属真菌约100余种，分布最广的为赤芝，其次为紫芝，还有树舌)、松杉灵芝和簿树芝等均供药用。经过大量临床研究，灵芝对神经衰弱、高脂血症、冠心病心绞痛、心律失常、克山病、高原不适症、肝炎、出血热、消化不良、气管炎等各有不同程度的疗效。药理研究证明，灵芝有多方面的生物活性。主要含氨基酸、多肽、蛋白质、真菌溶菌酶，以及糖类(还原糖和多糖)、麦角甾醇、三萜类、香豆 精甙、挥发油、硬脂酸、苯甲酸、生物碱、维生素B2及C等；孢子还含甘露醇、海藻糖。
发明内容：
本发明配方及用法：灵芝600克，经0.9％食盐水浸泡3min，饮用水冲洗一遍后打碎，渣汁加蜂蜜100mL，菌种100mL，饮用水10L。30摄氏度厌氧发酵5-10d。过滤备用。菌种用市售干酪乳杆菌、鼠李糖乳杆菌，嗜酸乳杆菌，单独MRS培养基[培养基配方(g/L)：牛肉蛋白粉10,鱼肉汁10,酵母浸出汁粉5,葡萄糖20,醋酸钠5,柠檬酸二铵2,吐温80 0.1,硫酸镁0.58,硫酸锰0.28,蒸溜水1000ml,121℃高压灭菌15min，调节pH6.2～6.4]培养18h按1:1:1混合做种子。每次早晚各饮用50-100mL，可加蜂蜜、低聚果糖、低聚异麦芽糖、白砂糖、乳糖酸等调味。常服该酵素可益气血、安心神、健脾胃，防治高血压。
本发明的技术方案：
本发明的目的是提出一种中药配方饮料制备方法，其无需昂贵设备，能有效避免真菌及致病菌毒素的产生，且能稳定持久具有良好的保健功效。
市售干酪乳杆菌、鼠李糖乳杆菌，嗜酸乳杆菌，单独MRS培养基培养18h按1:1:1混合为菌种，利用灵芝发酵制备酵素饮料。分为三个阶段，第一阶段是原料清洗，第二阶段为厌氧发酵，第三阶段为调味。实施工艺为：
1)取灵芝600克。水洗干净、经0.9％食盐水浸泡3分钟，饮用水冲洗一遍。
2)用市售干酪乳杆菌、鼠李糖乳杆菌，嗜酸乳杆菌，单独MRS培养基培养18h按1:1:1混合做种子。
3)适当打碎后加蜂蜜和菌种各100毫升，饮用水10升。30摄氏度厌氧发酵5-10天。过滤备用。
4)加适量蜂蜜、低聚果糖、低聚异麦芽糖、白砂糖、乳糖酸等调味。 本发明的有益效果：
本发明提供了一种具有保健功效的酵素饮料生产方法。本发明制备方法及过程中无污染安全可靠，发酵时间周期短，工艺环节少，简单易操作，可节省投资和运行费用。本发明可有效防止杂菌滋生，外加菌种可带来额外保健作用，使大分子物质迅速分解成人体容易吸收利用的小分子物质，有效提高酵素饮料的各种保健功效和高附加值。
具体实施方式：
下面结合实施例对本发明进行进一步描述。以下实施例仅为本发明的几个具体实施例，但本发明的设计构思并不局限于此，凡利用此构思对本发明进行非实质性的改动，均应属于侵犯本发明保护范围的行为。
实施例1
灵芝600克。水洗干净、经0.9％食盐水浸泡3分钟，饮用水冲洗一遍。菌种用市售干酪乳杆菌、鼠李糖乳杆菌，嗜酸乳杆菌，单独MRS培养基培养18h按1:1:1混合做种子。将原料适当打碎后加蜂蜜和菌种各100毫升，饮用水10升。30℃厌氧发酵8天。过滤后加蜂蜜、果葡糖浆各10g/L调味。得到口感风味具佳，保健作用良好的灵芝酵素饮料。
实施例2
灵芝400克。水洗干净、经0.9％食盐水浸泡3分钟，饮用水冲洗一遍。菌种用市售干酪乳杆菌、鼠李糖乳杆菌，嗜酸乳杆菌，单独MRS培养基培养18h按1:1:1混合做种子。将原料适当打碎后加蜂蜜和菌种各100毫升，饮用水10升。30℃厌氧发酵8天。过滤后加蜂蜜15g/L调味。得到口感风味具佳，保健作用良好的灵芝酵素饮料。
实施例3
灵芝400克。水洗干净、经0.9％食盐水浸泡3分钟，饮用水冲洗一遍。菌种用市售干酪乳杆菌、鼠李糖乳杆菌，嗜酸乳杆菌，单独MRS培养基培养18h按1:1:1混合做种子。将原料适当打碎后加蜂蜜和菌种各100毫升，饮用水10升。30℃厌氧发酵8天。过滤后加果葡糖浆各15g/L调味。得到口感风味具佳，保健作用良好的灵芝酵素饮料。
实施例4
灵芝600克。水洗干净、经0.9％食盐水浸泡3分钟，饮用水冲洗一遍。菌种用市售干酪乳杆菌、鼠李糖乳杆菌，嗜酸乳杆菌，单独MRS培养基培养18h按1:1:1混合做种子。将原料适当打碎后加蜂蜜和菌种各100毫升，饮用水10升。30℃厌氧发酵8天。过滤后加蔗糖10g/L调味。得到口感风味具佳，保健作用良好的灵芝酵素饮料。
Claims (8)
1.一种灵芝发酵制备酵素的方法，其特点是以灵芝为原料，添加菌种和蜂蜜发酵制备茶饮料，该方法包括以下步骤：
a)取灵芝600克。水洗干净、经0.9％食盐水浸泡3分钟，饮用水冲洗一遍。
b)用市售干酪乳杆菌、鼠李糖乳杆菌，嗜酸乳杆菌，单独MRS培养基培养18h按1:1:1混合做种子。
c)适当打碎后加蜂蜜和菌种各100毫升，饮用水10升。30摄氏度厌氧发酵5-10天。过滤备用。
d)加适量蜂蜜、低聚果糖、低聚异麦芽糖、白砂糖、乳糖酸等调味。
2.根据权利要求1所述的一种灵芝发酵制备酵素的方法，其特征是：所述菌种单独MRS培养基培养18h按1:1:1混合后做为酵素发酵用种子。
3.根据权利要求1所述的一种灵芝发酵制备酵素的方法，其特征是：菌种用MRS培养基配方为(g/L)：牛肉蛋白粉10,鱼肉汁10,酵母浸出汁粉5,葡萄糖20,醋酸钠5,柠檬酸二铵2,吐温80 0.1,硫酸镁0.58,硫酸锰0.28,蒸溜水1000ml,121℃高压灭菌15min，调节pH6.2～6.4。
4.根据权利要求1所述的一种灵芝发酵制备酵素的方法，其特征是：原料为灵芝，处理方法为：水洗干净、经0.9％食盐水浸泡3分钟，饮用水冲洗一遍。
5.根据权利要求1所述的一种灵芝发酵制备酵素的方法，其特征是：原料与蜂蜜、菌种的比例为每份原料(灵芝600克)适当打碎后加蜂蜜和菌种各100毫升，饮用水10升。
6.根据权利要求1所述的一种灵芝发酵制备酵素的方法，其特征是：原料及菌种、蜂蜜混合均匀30摄氏度厌氧发酵5-10天。过滤备用。
7.根据权利要求1所述的一种灵芝发酵制备酵素的方法，其特征是：过滤后发酵液添加适量蜂蜜、低聚果糖、低聚异麦芽糖、白砂糖、乳糖酸等调味。
8.根据权利要求1所述的一种灵芝发酵制备酵素的方法，其特征是：发酵过程中不会受杂菌污染，发酵后酵素品质良好，口感良好。
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610521835.0A CN106071605A (zh) | 2016-07-04 | 2016-07-04 | 一种灵芝发酵制备酵素的方法 |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610521835.0A CN106071605A (zh) | 2016-07-04 | 2016-07-04 | 一种灵芝发酵制备酵素的方法 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106071605A true CN106071605A (zh) | 2016-11-09 |
Family
ID=57213013
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610521835.0A Pending CN106071605A (zh) | 2016-07-04 | 2016-07-04 | 一种灵芝发酵制备酵素的方法 |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106071605A (zh) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106616973A (zh) * | 2016-11-10 | 2017-05-10 | 江苏丹枫神源生物科技有限公司 | 一种元宝枫翅果活菌酵素原液的制备方法 |
| CN108077908A (zh) * | 2017-11-28 | 2018-05-29 | 昆明理工大学 | 一种清热消炎的三七茎叶酵素的制备方法及其应用 |
| CN111728201A (zh) * | 2020-07-03 | 2020-10-02 | 杭州雪域生物技术有限公司 | 一种灵芝酵素及其制备方法与应用 |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103844266A (zh) * | 2014-03-25 | 2014-06-11 | 容瑜 | 一种天然调理养生酵素及其制造方法 |
| CN104522557A (zh) * | 2014-12-15 | 2015-04-22 | 深圳先进技术研究院 | 一种无糖酵素及其制备方法 |
| CN104824548A (zh) * | 2015-04-09 | 2015-08-12 | 周学义 | 枸杞复合保健酵素及其制备方法 |
| CN105495244A (zh) * | 2016-01-08 | 2016-04-20 | 江西果果生物科技有限公司 | 柚子苹果番茄酵素保健饮料的制作方法 |
-
2016
- 2016-07-04 CN CN201610521835.0A patent/CN106071605A/zh active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103844266A (zh) * | 2014-03-25 | 2014-06-11 | 容瑜 | 一种天然调理养生酵素及其制造方法 |
| CN104522557A (zh) * | 2014-12-15 | 2015-04-22 | 深圳先进技术研究院 | 一种无糖酵素及其制备方法 |
| CN104824548A (zh) * | 2015-04-09 | 2015-08-12 | 周学义 | 枸杞复合保健酵素及其制备方法 |
| CN105495244A (zh) * | 2016-01-08 | 2016-04-20 | 江西果果生物科技有限公司 | 柚子苹果番茄酵素保健饮料的制作方法 |
Non-Patent Citations (1)
| Title |
|---|
| 倪娜主编: "《食品加工实验指导》", 30 June 2015, 中国标准出版社 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106616973A (zh) * | 2016-11-10 | 2017-05-10 | 江苏丹枫神源生物科技有限公司 | 一种元宝枫翅果活菌酵素原液的制备方法 |
| CN108077908A (zh) * | 2017-11-28 | 2018-05-29 | 昆明理工大学 | 一种清热消炎的三七茎叶酵素的制备方法及其应用 |
| CN111728201A (zh) * | 2020-07-03 | 2020-10-02 | 杭州雪域生物技术有限公司 | 一种灵芝酵素及其制备方法与应用 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN109717340B (zh) | 一种结合复合酶解的两步式蛹虫草酵素的发酵制备方法 | |
| CN104544086B (zh) | 一种可改善肠道菌群的保健食品及其制备方法 | |
| CN106498018B (zh) | 一种复合菌制备人参稀有抗癌皂苷Compound K的方法 | |
| CN104013657B (zh) | 一种西洋参药材提取皂苷后微生物发酵再提取方法 | |
| KR101370386B1 (ko) | 유익균을 이용한 발효홍삼의 제조방법 | |
| CN103263448B (zh) | 用于发酵预处理改进银杏叶黄酮提取的发酵菌种及应用 | |
| WO2015143783A1 (zh) | 一种天然调理养生酵素及其制造方法 | |
| CN108244432A (zh) | 一种发酵蛹虫草益生菌饮料及其制备方法 | |
| CN1298259C (zh) | 一种灵芝发芽糙米果液的生产方法及其产品 | |
| KR101753077B1 (ko) | 발효 인삼추출물의 제조방법 및 이를 함유하는 조성물 | |
| CN103907987A (zh) | 一种多菌种混合连续发酵制备山楂果醋饮料的方法 | |
| CN107753741B (zh) | 黄精精深加工方法及其制品 | |
| KR101823585B1 (ko) | 발효 인삼추출물의 제조방법 | |
| CN101603000B (zh) | 一种富含γ-氨基丁酸醋的制备方法 | |
| CN110507681A (zh) | 一种高值化利用人参花的工艺 | |
| CN109566933A (zh) | 一种发酵复方饮品及其制备方法 | |
| CN105919113A (zh) | 一种微生物发酵制备灵豆酵素的方法 | |
| CN106071605A (zh) | 一种灵芝发酵制备酵素的方法 | |
| CN106434755A (zh) | 桦褐孔菌深层发酵产物及用途 | |
| CN106174500A (zh) | 一种红枣复合酵素的制作方法 | |
| CN104357332B (zh) | 黑曲霉jh‑2及在生物转化合成积雪草酸中应用 | |
| CN105062834A (zh) | 食药用菌高酒度红曲黑米酒的制作方法 | |
| CN107488616A (zh) | 菌种、益生菌面膜、养生菌菌茶以及食用菌菌茶的制备方法 | |
| CN105998222A (zh) | 益生菌发酵生附子的组合物及其制备方法和应用 | |
| CN107668696A (zh) | 一种灵芝酵素的制备方法 |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20161109 |
|
| WD01 | Invention patent application deemed withdrawn after publication |