US20200368307A1 - Composition containing plant extracts and the use thereof - Google Patents

Composition containing plant extracts and the use thereof Download PDF

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Publication number
US20200368307A1
US20200368307A1 US16/500,332 US201816500332A US2020368307A1 US 20200368307 A1 US20200368307 A1 US 20200368307A1 US 201816500332 A US201816500332 A US 201816500332A US 2020368307 A1 US2020368307 A1 US 2020368307A1
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Prior art keywords
extract
tea
blueberry
composition
collagen
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US16/500,332
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English (en)
Inventor
Yung-Hsiang Lin
I-Hui Chen
Kai-Wen KAN
Fu Chen LIU
Ciao-Ting Chen
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TCI Co Ltd
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TCI Co Ltd
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Priority to US16/500,332 priority Critical patent/US20200368307A1/en
Assigned to TCI CO., LTD reassignment TCI CO., LTD ASSIGNMENT OF ASSIGNORS INTEREST (SEE DOCUMENT FOR DETAILS). Assignors: CHEN, CIAO-TING, CHEN, I-HUI, KAN, Kai-Wen, LIN, YUNG-HSIANG, LIU, FU CHEN
Publication of US20200368307A1 publication Critical patent/US20200368307A1/en
Abandoned legal-status Critical Current

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    • A61K9/0053—Mouth and digestive tract, i.e. intraoral and peroral administration
    • A61K9/0056—Mouth soluble or dispersible forms; Suckable, eatable, chewable coherent forms; Forms rapidly disintegrating in the mouth; Lozenges; Lollipops; Bite capsules; Baked products; Baits or other oral forms for animals
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Definitions

  • the present invention relates to a composition containing plant extracts and the use thereof, and more particularly to the composition containing plant extracts and the use thereof for enhancing the synthesis of collagen.
  • Collagen is the most important structural protein in animals and the main component of extracellular matrix and connective tissue. Collagen is widely found in tissues such as tendons, bones, skin and cornea and is the most abundant protein in animals which accounts for 20-30% of the total protein content and 6% of the total body weight of the animal.
  • collagen can be synthesized by the body itself.
  • the synthesis of collagen decreases and the loss rate of collagen increases with age, and problems of loss of collagen would happen such as loose skin or joint wear.
  • the amount of collagen secretion in the body can be properly increased, the skin can be kept elastic, the formation of wrinkles can be slowed down, the strength and the flexibility of joints can be increased, and the wear and aging of joints can be slowed down.
  • the ingredients or drugs currently available for promoting the increase in the amount of collagen secretion are limited. If a certain plant extract is used to promote the increase in the amount of collagen secretion, it must be administered at a relatively high dose, and thus there are limitations in the cost of use and effect.
  • composition containing plurality of plant extracts with a small amount of each component can be administered to produce the similar effect when administered a high-dose of a single component, the use of the aforementioned effects can be greatly improved, and at the same time, the use cost can be reduced.
  • one objective of the present invention is to provide a composition comprising a plant extract, wherein the plant extract comprises at least one combination selected from the group consisting of a red wine extract and a blueberry extract, a citrus extract and a blueberry extract, a citrus extract and a Four Seasons Spring tea extract, a spinach extract and a black tea extract, a spinach extract and a green tea extract, a green coffee bean extract and a blueberry extract, and a green coffee bean extract and a Pu-erh tea extract.
  • the composition comprises any of the following combinations: at least 0.003906 mg/mL of the green coffee bean extract and at least 0.003906 mg/mL of the blueberry extract; at least 0.015625 mg/mL of the green coffee bean extract and at least 0.015625 mg/mL of the Pu-erh tea extract; at least 0.125 mg/mL of the blueberry extract and at least 0.125 mg/mL of the red wine extract; at least 0.25 mg/mL of the spinach extract and at least 0.25 mg/mL of the green tea extract; at least 0.5 mg/mL of the spinach extract and at least 0.5 mg/mL of the black tea extract; at least 0.5 mg/mL of the citrus extract and at least 0.5 mg/mL of the Four Seasons Spring tea extract, and at least 4 mg/mL of the citrus extract and at least 4 mg/mL of the blueberry extract.
  • the other objective of the present invention is to provide a pharmaceutical composition for enhancing the synthesis of collagen, comprising the aforementioned composition and a pharmaceutical acceptable carrier.
  • the pharmaceutical composition is in a form of a solution, a capsule or a tablet.
  • Another objective of the present invention is to provide a food composition for enhancing the synthesis of collagen, comprising the aforementioned composition and a food ingredient, wherein the food ingredient is a component of a general food, a health food, a dietary supplement, or a drink.
  • Another objective of the present invention is to provide a method for enhancing the synthesis of collagen, comprising administering to a subject in need thereof an effective amount of the aforementioned composition, wherein the composition is a food product.
  • composition comprising plant extracts of the present invention can effectively promote the synthesis of collagen, effectively enhance the synthesis rate of collagen, enhance the elasticity of the skin, reduce the wrinkle formation, and improve the strength and flexibility of the joint.
  • FIG. 1 is a diagram showing the effects of different plant extracts or combinations on the enhancement of synthesis rate of collagen.
  • the data provided represent experimental values that can vary within a range of ⁇ 20%, preferably within ⁇ 10%, and most preferably within ⁇ 5%.
  • the Four Seasons Spring tea extract was prepared as follows: first, the leaves of Four Seasons Spring tea plant were washed and dried, and then Four Seasons Spring tea were coarsely crushed by a pulverizer to be a crude material.
  • the crude material of Four Seasons Spring tea was extracted using water as the solvent.
  • the weight ratio of the solvent to the crude material was 5-20:1-5.
  • the extraction temperature was at 50-100° C., preferably 75-95° C., for 0.5-3 hours to obtain the crude extract of the Four Seasons Spring tea.
  • the crude extraction was cooled to room temperature.
  • the crude extraction was filtered through a 400 mesh filter to remove residual solids.
  • the filtered of the Four Seasons Spring tea was further concentrated under reduced pressure at 45-70° C. to obtain a concentrated product and the concentrated product was the Four Seasons Spring tea extract of the present invention.
  • the green tea extract was prepared as follows: first, the unfermented leaves of Camellia sinensis, i.e. the green tea, were washed and dried, and then were coarsely crushed by a pulverizer to be a crude material.
  • the crude material of green tea was extracted using water as the solvent.
  • the weight ratio of the solvent to the crude material was 5-20:1-5.
  • the extraction temperature was at 50-100° C., preferably 75-95° C., for 0.5-3 hours to obtain the crude extract of the green tea.
  • the crude extraction was cooled to room temperature.
  • the crude extraction was filtered through a 400 mesh filter to remove residual solids.
  • the filtered of the green tea was further concentrated under reduced pressure at 45-70° C. to obtain a concentrated product and the concentrated product was the green tea extract of the present invention.
  • the black tea extract was prepared as follows: first, the fermented leaves of Camellia sinensis, i.e. the black tea, were washed and dried, and then were coarsely crushed by a pulverizer to be a crude material.
  • the crude material of black tea was extracted using water as the solvent.
  • the weight ratio of the solvent to the crude material was 5-20:1-5.
  • the extraction temperature was at 50-100° C., preferably 75-95° C., for 0.5-3 hours to obtain the crude extract of the black tea.
  • the crude extraction was cooled to room temperature.
  • the crude extraction was filtered through a 400 mesh filter to remove residual solids.
  • the filtered of the black tea was further concentrated under reduced pressure at 45-70° C. to obtain a concentrated product and the concentrated product was the black tea extract of the present invention.
  • the spinach extract was obtained by extracting spinach ( Spinacia oleracea ), and the spinach extract used in the example was purchased from Honh Siang Farm Products Factory.
  • the red wine extract was obtained by extracting red wine, and the red wine extract used in the example was purchased from Shanghai Boyoutang Biotechnology Co., Ltd.
  • the citrus extract was obtained by extracting the fruits of Citrus reticulata, and the citrus extract used in the example was purchased from Roterm Trading Co., Ltd.
  • the green coffee bean extract was obtained by extracting the seeds of unroasted coffee ( Coffea spp.), and the green coffee bean extract used in the example was purchased from Arjuna Natural Extracts Ltd.
  • the blueberry extract was obtained by extracting the fruits of Vaccinium Cyanococcus, and the blueberry extract used in the example was purchased from BIOMED HERBAL RESEARCH CO., LTD.
  • the Pu-erh tea extract was obtained by extracting the post-fermented leaves of Camellia sinensis, and the Pu-erh tea extract used in the example was purchased from Nanjing Zelang Biotechnology Co., Ltd.
  • the grape seed extract was purchased from Guarante Biotech Co., Ltd.
  • the present invention analyzed the synthesis of collagen by human skin fibroblast (CCD-966sk).
  • the human skin fibroblast was purchased from the Food Industry Research and Development Institute, Taiwan, and the number is BCRC 60153.
  • the cells were cultured in the culture medium with 10% fetal bovine serum and 90% minimum essential medium (MEM, purchased from Gibco, USA) and the medium further contains 1 mM sodium pyruvate and 1% penicillin or streptomycin.
  • the synthesis rate of collagen in the groups of the combination of the green coffee bean extract and the blueberry extract, the combination of the green coffee bean extract and the Pu-erh tea extract, the combination of the red wine extract and the blueberry extract, the combination of the spinach extract and the green tea extract, the combination of the spinach extract and the black tea extract, the combination of the citrus extract and the Four Seasons Spring tea extract, and the combination of the citrus extract and the blueberry extract were all enhanced much more than that in each group of single plant extract with same concentration. The result indicates that the specific combinations described above unexpectedly exhibit a higher ability to enhance the synthesis of collagen than a single plant extract, and not a random combination can achieve such effect.
  • compositions of the present invention significantly enhance the synthesis collagen by mixing the specific plant extracts or mixing the specific plant extracts with the plant-derived compounds. Therefore, the composition can be utilized to prepare pharmaceutical or food compositions that enable to enhance the elasticity of the skin, reduce the wrinkle formation, and improve the strength and flexibility of the joint of the applicator or consumer.
  • composition can be further added to a food, a health food, a dietary supplement, or a drink.
  • a food a health food, a dietary supplement, or a drink.
  • the composition for enhancing the synthesis of collagen of the embodiment of the present invention is prepared to be a pharmaceutical composition
  • the pharmaceutical composition can be further added to a carrier or other adjuvant well known in the art.
  • the form of the pharmaceutical composition can be, but is not limited to, a solution, a capsule, or a tablet.

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