PT1179600E - Monitorizacao da hibridacao durante a rcp - Google Patents

Monitorizacao da hibridacao durante a rcp

Info

Publication number
PT1179600E
PT1179600E PT01203467T PT01203467T PT1179600E PT 1179600 E PT1179600 E PT 1179600E PT 01203467 T PT01203467 T PT 01203467T PT 01203467 T PT01203467 T PT 01203467T PT 1179600 E PT1179600 E PT 1179600E
Authority
PT
Portugal
Prior art keywords
nucleic acid
target nucleic
acid sequence
biological sample
hibridation
Prior art date
Application number
PT01203467T
Other languages
English (en)
Portuguese (pt)
Inventor
Carl T Wittwer
Kirk M Ririe
Randy P Rasmussen
Original Assignee
Univ Utah Res Found
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=27097737&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=PT1179600(E) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Univ Utah Res Found filed Critical Univ Utah Res Found
Publication of PT1179600E publication Critical patent/PT1179600E/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
    • B01L3/5082—Test tubes per se
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L7/00—Heating or cooling apparatus; Heat insulating devices
    • B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6823—Release of bound markers
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
    • G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
    • G01N21/251—Colorimeters; Construction thereof
    • G01N21/253—Colorimeters; Construction thereof for batch operation, i.e. multisample apparatus
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/6452—Individual samples arranged in a regular 2D-array, e.g. multiwell plates
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0627—Sensor or part of a sensor is integrated
    • B01L2300/0654—Lenses; Optical fibres
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0832—Geometry, shape and general structure cylindrical, tube shaped
    • B01L2300/0838—Capillaries
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/18—Means for temperature control
    • B01L2300/1838—Means for temperature control using fluid heat transfer medium
    • B01L2300/1844—Means for temperature control using fluid heat transfer medium using fans
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N2021/6417—Spectrofluorimetric devices
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N2021/6482—Sample cells, cuvettes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N2035/00178—Special arrangements of analysers
    • G01N2035/00237—Handling microquantities of analyte, e.g. microvalves, capillary networks
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/01—Arrangements or apparatus for facilitating the optical investigation
    • G01N21/03—Cuvette constructions
    • G01N21/0303—Optical path conditioning in cuvettes, e.g. windows; adapted optical elements or systems; path modifying or adjustment
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/01—Arrangements or apparatus for facilitating the optical investigation
    • G01N21/03—Cuvette constructions
    • G01N21/07—Centrifugal type cuvettes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/02—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations
    • G01N35/025—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations having a carousel or turntable for reaction cells or cuvettes
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/14—Heterocyclic carbon compound [i.e., O, S, N, Se, Te, as only ring hetero atom]
    • Y10T436/142222—Hetero-O [e.g., ascorbic acid, etc.]
    • Y10T436/143333—Saccharide [e.g., DNA, etc.]

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Immunology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Molecular Biology (AREA)
  • General Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Pathology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Clinical Laboratory Science (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Hematology (AREA)
  • Optics & Photonics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Fats And Perfumes (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
PT01203467T 1996-06-04 1997-06-04 Monitorizacao da hibridacao durante a rcp PT1179600E (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US65899396A 1996-06-04 1996-06-04
US81826797A 1997-03-17 1997-03-17

Publications (1)

Publication Number Publication Date
PT1179600E true PT1179600E (pt) 2005-08-31

Family

ID=27097737

Family Applications (2)

Application Number Title Priority Date Filing Date
PT01203467T PT1179600E (pt) 1996-06-04 1997-06-04 Monitorizacao da hibridacao durante a rcp
PT97931089T PT912766E (pt) 1996-06-04 1997-06-04 Monitorização da hibridização durante a pcr

Family Applications After (1)

Application Number Title Priority Date Filing Date
PT97931089T PT912766E (pt) 1996-06-04 1997-06-04 Monitorização da hibridização durante a pcr

Country Status (12)

Country Link
US (9) US6174670B1 (cg-RX-API-DMAC7.html)
EP (3) EP1179600B1 (cg-RX-API-DMAC7.html)
JP (4) JP4540754B2 (cg-RX-API-DMAC7.html)
AT (3) ATE318327T1 (cg-RX-API-DMAC7.html)
AU (1) AU726501B2 (cg-RX-API-DMAC7.html)
CA (3) CA2257109C (cg-RX-API-DMAC7.html)
DE (3) DE69735313T2 (cg-RX-API-DMAC7.html)
DK (2) DK1179600T3 (cg-RX-API-DMAC7.html)
ES (2) ES2243393T3 (cg-RX-API-DMAC7.html)
NZ (2) NZ502323A (cg-RX-API-DMAC7.html)
PT (2) PT1179600E (cg-RX-API-DMAC7.html)
WO (1) WO1997046714A1 (cg-RX-API-DMAC7.html)

Families Citing this family (801)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20070269799A9 (en) * 1994-06-22 2007-11-22 Zhang David Y Nucleic acid amplification methods
ATE318327T1 (de) * 1996-06-04 2006-03-15 Univ Utah Res Found Fluoreszenz-donor-akzeptor paar
WO1998036096A1 (en) * 1997-02-14 1998-08-20 E.I. Du Pont De Nemours And Company Detection of double-stranded dna in a homogeneous solution
US6518017B1 (en) * 1997-10-02 2003-02-11 Oasis Biosciences Incorporated Combinatorial antisense library
US20030165888A1 (en) * 2001-07-18 2003-09-04 Brown Bob D. Oligonucleotide probes and primers comprising universal bases for diagnostic purposes
US6485901B1 (en) 1997-10-27 2002-11-26 Boston Probes, Inc. Methods, kits and compositions pertaining to linear beacons
AU1366299A (en) 1997-10-27 1999-05-17 Boston Probes, Inc. Methods, kits and compositions pertaining to pna molecular beacons
PT1040202E (pt) * 1997-10-28 2009-03-26 Los Alamos Nat Security Llc Determinação da identidade de polimorfismos de adn utilizando citometria de fluxo
WO1999023250A2 (de) * 1997-11-04 1999-05-14 Roche Diagnostics Gmbh Spezifisches und sensitives nukleinsäurenachweisverfahren
GB9725197D0 (en) * 1997-11-29 1998-01-28 Secr Defence Detection system
US6893877B2 (en) * 1998-01-12 2005-05-17 Massachusetts Institute Of Technology Methods for screening substances in a microwell array
WO1999040219A1 (en) * 1998-02-05 1999-08-12 Bavarian Nordic Research Institute A/S Quantification by inhibition of amplification
GB9803382D0 (en) 1998-02-19 1998-04-15 Secr Defence Detection system
US6270973B1 (en) * 1998-03-13 2001-08-07 Promega Corporation Multiplex method for nucleic acid detection
DE19811731A1 (de) * 1998-03-18 1999-09-23 November Ag Molekulare Medizin Verfahren zum Nachweis einer Nukleotidsequenz
DE19811729C2 (de) * 1998-03-18 2000-05-18 November Ag Molekulare Medizin Verfahren und Vorrichtung zum Nachweis einer Nukleotidsequenz
WO1999049293A2 (en) 1998-03-24 1999-09-30 Boston Probes, Inc. Methods, kits and compositions pertaining to detection complexes
US6132968A (en) * 1998-05-13 2000-10-17 University Of Alberta Methods for quantitating low level modifications of nucleotide sequences
GB9811483D0 (en) * 1998-05-29 1998-07-29 Photonic Research Systems Limi Luminescence assay using cyclical excitation wavelength sequence
US6780617B2 (en) 2000-12-29 2004-08-24 Chen & Chen, Llc Sample processing device and method
US7799521B2 (en) * 1998-06-24 2010-09-21 Chen & Chen, Llc Thermal cycling
GB9815799D0 (en) * 1998-07-21 1998-09-16 Pharmagene Lab Limited Quantitative analysis of gene expression
GB9819417D0 (en) 1998-09-07 1998-10-28 Secr Defence Reaction method
GB9819418D0 (en) 1998-09-07 1998-10-28 Secr Defence Amplification method
DE19840897A1 (de) * 1998-09-08 2000-03-09 Dirk Happich Faktor V-Leiden-Test
US6140054A (en) * 1998-09-30 2000-10-31 University Of Utah Research Foundation Multiplex genotyping using fluorescent hybridization probes
GB9821989D0 (en) * 1998-10-08 1998-12-02 Hybaid Ltd Detection of nucleic acid polymorphism
DE19850593A1 (de) * 1998-11-03 2000-05-04 Biochip Technologies Gmbh Verfahren zum Nachweis von Hybridisierungsereignissen bei DNA
US6216049B1 (en) * 1998-11-20 2001-04-10 Becton, Dickinson And Company Computerized method and apparatus for analyzing nucleic acid assay readings
EP1013775A1 (en) * 1998-12-21 2000-06-28 LUTZ, Hans Quantitative polymerase chain reaction using a fluorogenic real-time detection system
GB9901072D0 (en) * 1999-01-19 1999-03-10 Imp Cancer Res Tech Methods for detecting changes to a macromolecular component of a cell
BR0009164A (pt) * 1999-03-19 2001-12-26 Genencor Int Placa de teste de múltiplos furos vazados paraseparação de alto desempenho
US6303305B1 (en) 1999-03-30 2001-10-16 Roche Diagnostics, Gmbh Method for quantification of an analyte
WO2000061810A1 (en) * 1999-04-08 2000-10-19 Oasis Biosciences, Inc. Antisense oligonucleotides comprising universal and/or degenerate bases
GB9908437D0 (en) * 1999-04-13 1999-06-09 Minton Treharne & Davies Limit Methods of marking materials
DE60045059D1 (de) * 1999-04-20 2010-11-18 Nat Inst Of Advanced Ind Scien Verfahren und Sonden zur Bestimmung der Konzentration von Nukleinsäure-Molekülen und Verfahren zur Analyse der gewonnenen Daten
US6387621B1 (en) * 1999-04-27 2002-05-14 University Of Utah Research Foundation Automated analysis of real-time nucleic acid amplification
US6331393B1 (en) 1999-05-14 2001-12-18 University Of Southern California Process for high-throughput DNA methylation analysis
EP1190097A2 (en) * 1999-06-22 2002-03-27 Invitrogen Corporation Improved primers and methods for the detection and discrimination of nucleic acids
CA2380238A1 (en) * 1999-07-27 2001-02-01 University Of Utah Research Foundation Homogeneous fluorescence method for assaying structural modifications of biomolecules
US6197520B1 (en) * 1999-08-13 2001-03-06 University Of Utah Research Foundation Solution-based color compensation adjusted for temperature and electronic gains
US6927203B1 (en) * 1999-08-17 2005-08-09 Purdue Research Foundation Treatment of metastatic disease
US7192698B1 (en) * 1999-08-17 2007-03-20 Purdue Research Foundation EphA2 as a diagnostic target for metastatic cancer
AU1933701A (en) * 1999-11-29 2001-06-04 Glaxo Group Limited Continuous time-resolved resonance energy-transfer assay for polynucleic acid polymerases
GB9928232D0 (en) * 1999-12-01 2000-01-26 Skelton Stephen Detection system
DE10027113A1 (de) * 1999-12-23 2001-09-27 Andreas Hoeft Verfahren zur Bestimmung von mikrobieller DNS/RNS, Kit dafür und Verwendung des Verfahrens
US7700279B2 (en) * 1999-12-24 2010-04-20 Quest Diagnostics Investments Incorporated Assay for bcr/abl gene rearrangement
US7250252B2 (en) * 1999-12-30 2007-07-31 David Aaron Katz Amplification based polymorphism detection
US20020151040A1 (en) 2000-02-18 2002-10-17 Matthew O' Keefe Apparatus and methods for parallel processing of microvolume liquid reactions
GB0005281D0 (en) * 2000-03-07 2000-04-26 Secr Defence Analytical method
DE10045521A1 (de) * 2000-03-31 2001-10-04 Roche Diagnostics Gmbh Nukleinsäureamplifikationen
US6691041B2 (en) * 2000-03-31 2004-02-10 Roche Molecular Systems, Inc. Method for the efficiency-corrected real-time quantification of nucleic acids
DE10018465A1 (de) * 2000-04-14 2001-10-18 Aventis Res & Tech Gmbh & Co Testsystem zum Nachweis einer Spleißreaktion, sowie dessen Verwendung
US6783934B1 (en) * 2000-05-01 2004-08-31 Cepheid, Inc. Methods for quantitative analysis of nucleic acid amplification reaction
EP1158449A3 (en) * 2000-05-19 2004-12-15 Becton Dickinson and Company Computerized method and apparatus for analyzing readings of nucleic acid assays
GB0016516D0 (en) * 2000-07-06 2000-08-23 Owen Mumford Ltd Improvements relating to needle protection devices
BR0112261B1 (pt) * 2000-07-07 2012-10-02 processo para seqüenciamento de dna mediado por transposon, paralelo.
ATE332398T1 (de) 2000-08-11 2006-07-15 Univ Utah Res Found Einfach markierte oligonukleotidsonden
EP1360324A2 (en) 2000-08-31 2003-11-12 Mayo Foundation For Medical Education And Research Detection of varicella-zoster virus
US7101976B1 (en) 2000-09-12 2006-09-05 Purdue Research Foundation EphA2 monoclonal antibodies and methods of making and using same
US6558929B2 (en) * 2000-09-15 2003-05-06 Fraunhofer-Gesellschaft Zur Forderung Der Angewandten Forshung E.V. PCR reaction mixture for fluorescence-based gene expression and gene mutation analyses
US6346386B1 (en) * 2000-09-29 2002-02-12 Arup Institue Method of solution-based scanning for alterations in a DNA segment using a double-stranded DNA binding dye and fluorescence melting profiles
GB0025913D0 (en) * 2000-10-23 2000-12-06 Guldberg Per Materials and methods relating to nucleic acid amplification and profiling
ATE445023T1 (de) * 2000-10-23 2009-10-15 Cancer Rec Tech Ltd Auf nukleinsäurenamplifizierung basierendes verfahren zur bestimmung von methylierungsprofil und reagenzien dafür
US8900811B2 (en) 2000-11-16 2014-12-02 Caliper Life Sciences, Inc. Method and apparatus for generating thermal melting curves in a microfluidic device
ES2245995T3 (es) * 2000-11-16 2006-02-01 F. Hoffmann-La Roche Ag Pares de colorantes para mediciones de transferencia de energia de fluorescencia-resonancia (fret).
US6312929B1 (en) * 2000-12-22 2001-11-06 Cepheid Compositions and methods enabling a totally internally controlled amplification reaction
US7691571B2 (en) * 2001-01-31 2010-04-06 Mayo Foundation For Medical Education And Research Detection of bordetella
WO2002061141A1 (en) * 2001-01-31 2002-08-08 Mayo Foundation For Medical Education And Research Detection of bordetella
AU2002243751A1 (en) * 2001-01-31 2002-08-12 Mayo Foundation For Medical Education And Research Detection of herpes simplex virus
US7267945B2 (en) * 2001-03-26 2007-09-11 Applera Corporation Methods of determining the presence of polynucleotides employing amplification
US7559879B2 (en) * 2001-04-16 2009-07-14 Brunswick Corporation Stride adjustment mechanism
WO2002089844A1 (en) 2001-05-07 2002-11-14 Mayo Foundation For Medical Education And Research Legionella detection with fluorescent resonance energy transfer
US6979530B2 (en) 2001-05-21 2005-12-27 Applera Corporation Peptide conjugates and fluorescence detection methods for intracellular caspase assay
GB0112868D0 (en) 2001-05-25 2001-07-18 Secr Defence Detection system
WO2003002959A1 (en) * 2001-06-15 2003-01-09 Mj Research, Inc. Controller for a fluorometer
WO2003000935A1 (en) * 2001-06-22 2003-01-03 Marshfield Clinic Methods and oligonucleotides for the detection of salmonella sp.,e.coli o157:h7,and listeria monocytogenes
DE10132211A1 (de) * 2001-06-27 2003-01-16 Epigenomics Ag Nachweis spezifischer Dinukleotide in DNA-Proben durch Fluoreszenzresonanzenergietransfer (FRET)
US9261460B2 (en) 2002-03-12 2016-02-16 Enzo Life Sciences, Inc. Real-time nucleic acid detection processes and compositions
DE10132785A1 (de) * 2001-07-06 2003-01-16 Clondiag Chip Tech Gmbh Verfahren zum Nachweis von in einer Polymerase-Kettenreaktion amplifizierten Nukleinsäuremolekülen
EP1275735A1 (en) 2001-07-11 2003-01-15 Roche Diagnostics GmbH Composition and method for hot start nucleic acid amplification
US7226732B2 (en) * 2001-07-16 2007-06-05 Cepheid Methods, apparatus, and computer programs for verifying the integrity of a probe
CA2450343C (en) * 2001-07-16 2013-12-17 Idaho Technology, Inc. Thermal cycling system and method of use
US6872529B2 (en) * 2001-07-25 2005-03-29 Affymetrix, Inc. Complexity management of genomic DNA
US6979541B1 (en) 2001-07-26 2005-12-27 University Of Utah Research Foundation Methods for identifying chromosomal aneuploidy
US7188030B2 (en) * 2001-08-21 2007-03-06 Applera Corporation Automatic threshold setting for quantitative polymerase chain reaction
CA2457888A1 (en) * 2001-08-23 2003-03-06 Merck & Co., Inc. Fluorescent multiplex hpv pcr assays using multiple fluorophores
CA2400601A1 (en) * 2001-08-31 2003-02-28 University Of Utah Research Foundation Real-time gene quantification with internal standards
AU2002341644B2 (en) * 2001-09-11 2008-02-28 Iquum, Inc. Sample vessels
US20030082563A1 (en) * 2001-10-15 2003-05-01 Bell Constance A. Detection of bacillus anthracis
US20030165859A1 (en) 2001-10-23 2003-09-04 Invitrogen Corporation Primers and methods for the detection and discrimination of nucleic acids
DE60211829T2 (de) * 2001-11-02 2007-05-16 Roche Diagnostics Gmbh Nachweis von Variola Virus
EP1456409B1 (en) * 2001-11-28 2010-02-24 Bio-Rad Laboratories, Inc. Parallel polymorphism scoring by amplification and error correction
US7424013B1 (en) * 2001-12-20 2008-09-09 Applied Micro Circuits Corporation System and method for granting arbitrated bids in the switching of information
US8418129B1 (en) 2001-12-14 2013-04-09 Qualcomm Incorporated Method for automatically generating code to define a system of hardware elements
US7352694B1 (en) * 2001-12-14 2008-04-01 Applied Micro Circuits Corporation System and method for tolerating data link faults in a packet communications switch fabric
US20040018506A1 (en) 2002-01-25 2004-01-29 Koehler Ryan T. Methods for placing, accepting, and filling orders for products and services
US7228237B2 (en) 2002-02-07 2007-06-05 Applera Corporation Automatic threshold setting and baseline determination for real-time PCR
US7373253B2 (en) 2002-02-12 2008-05-13 Idaho Technology Multi-test analysis of real-time nucleic acid amplification
US6593093B1 (en) 2002-02-20 2003-07-15 Mayo Foundation For Medical Education And Research Detection of group a Streptococcus
US7166478B2 (en) * 2002-03-12 2007-01-23 Enzo Life Sciences, Inc., C/O Enzo Biochem, Inc. Labeling reagents and labeled targets, target labeling processes and other processes for using same in nucleic acid determinations and analyses
US9353405B2 (en) 2002-03-12 2016-05-31 Enzo Life Sciences, Inc. Optimized real time nucleic acid detection processes
US20050065330A1 (en) * 2002-04-24 2005-03-24 The Cleveland Clinic Foundation Identification of Histoplasma capsulatum using a PCR assay
AU2003231121B2 (en) * 2002-04-26 2008-08-07 University Of Utah Research Foundation Characterization of single stranded nucleic acids by melting analysis using a double strand specific nucleic acid dye
AUPS205802A0 (en) * 2002-05-01 2002-06-06 Bio-Molecular Holdings Pty Limited Improved cycling device and method
US7785776B2 (en) 2002-05-13 2010-08-31 Idaho Technology, Inc. Genotyping by amplicon melting curve analysis
US20030215814A1 (en) * 2002-05-17 2003-11-20 Cockerill Franklin R. Detection of Shiga toxin- or Shiga-like toxin-producing organisms
AUPS267802A0 (en) 2002-05-30 2002-06-20 Bio-Molecular Holdings Pty Limited Improved dna amplification apparatus and method
JP4457001B2 (ja) * 2002-05-31 2010-04-28 セクレタリー・デパートメント・オブ・アトミック・エナジー ドナー／アクセプター部分が相補鎖上となる標的核酸検出のｍｅｔ／ｆｒｅｔに基づく方法
EP2292801B1 (en) * 2002-06-14 2018-08-08 Gen-Probe Incorporated Compositions and methods for detecting hepatitis b virus
US6896727B2 (en) * 2002-06-28 2005-05-24 Seh America, Inc. Method of determining nitrogen concentration within a wafer
US7083974B2 (en) * 2002-07-12 2006-08-01 Applera Corporation Rotatable sample disk and method of loading a sample disk
US20040219557A1 (en) * 2002-08-01 2004-11-04 Dobrowolski Steven F. Real time PCR assays to detect mutations in the biotinidase gene for newborn screening
US8277753B2 (en) * 2002-08-23 2012-10-02 Life Technologies Corporation Microfluidic transfer pin
AU2003236461B2 (en) * 2002-08-29 2009-05-28 Epigenomics Ag Improved method for bisulfite treatment
WO2004021986A2 (en) * 2002-09-03 2004-03-18 Quanta Biosciences, Inc. Improved compositions and methods for cdna synthesis
AU2003270568A1 (en) * 2002-09-17 2004-04-08 Pharmacia Corporation Preservation of rna and reverse transcriptase during automated liquid handling
JP4601427B2 (ja) * 2002-09-18 2010-12-22 ザ・トラステイーズ・オブ・ザ・ユニバーシテイ・オブ・ペンシルベニア 細胞上および生物学的混合物中の抗原の検出のための組成物、方法およびキット
US7074598B2 (en) 2002-09-25 2006-07-11 Mayo Foundation For Medical Education And Research Detection of vancomycin-resistant enterococcus spp.
US7365176B2 (en) 2002-09-26 2008-04-29 Mayo Foundation For Medical Education And Research Detection of Epstein-Barr virus
US7074599B2 (en) 2002-09-27 2006-07-11 Mayo Foundation For Medical Education And Research Detection of mecA-containing Staphylococcus spp.
US7919235B2 (en) * 2002-09-30 2011-04-05 F. Hoffman-La Roche Ag Oligonucleotides for genotyping thymidylate synthase gene
US9850381B2 (en) * 2002-10-23 2017-12-26 University Of Utah Research Foundation Amplicon melting analysis with saturation dyes
EP1558762B1 (en) * 2002-10-23 2013-09-11 University of Utah Research Foundation Amplicon melting analysis with saturation dyes
US7718361B2 (en) 2002-12-06 2010-05-18 Roche Molecular Systems, Inc. Quantitative test for bacterial pathogens
WO2004053155A1 (en) 2002-12-06 2004-06-24 Roche Diagniostics Gmbh Multiplex assay detection of pathogenic organisms
ATE386141T1 (de) * 2002-12-20 2008-03-15 Stratagene Inc Zusammensetzungen und verfahren für den nachweis von polynukleotiden
US7560231B2 (en) * 2002-12-20 2009-07-14 Roche Molecular Systems, Inc. Mannitol and glucitol derivatives
CA2510166A1 (en) 2002-12-20 2004-09-30 Caliper Life Sciences, Inc. Single molecule amplification and detection of dna
US8275554B2 (en) * 2002-12-20 2012-09-25 Caliper Life Sciences, Inc. System for differentiating the lengths of nucleic acids of interest in a sample
US20060094108A1 (en) * 2002-12-20 2006-05-04 Karl Yoder Thermal cycler for microfluidic array assays
AU2003302264A1 (en) * 2002-12-20 2004-09-09 Biotrove, Inc. Assay apparatus and method using microfluidic arrays
US20050042639A1 (en) * 2002-12-20 2005-02-24 Caliper Life Sciences, Inc. Single molecule amplification and detection of DNA length
ATE373673T1 (de) * 2003-01-29 2007-10-15 Hoffmann La Roche Verbessertes verfahren zur behandlung durch bisulfit
EP1597602A4 (en) * 2003-02-05 2009-07-22 Ge Healthcare Bio Sciences SOLID PHASE SEQUENCING
AU2004220626B2 (en) * 2003-02-05 2010-07-29 Iquum Inc. Sample processing tubule
US20040229253A1 (en) * 2003-02-18 2004-11-18 Hyldig-Nielsen Jens J. Compositions and methods for multiplex analysis of polynucleotides
US7282331B2 (en) * 2003-03-18 2007-10-16 Advandx, Inc. Method for improved specificity in probe based assays
EP1606419A1 (en) 2003-03-18 2005-12-21 Quantum Genetics Ireland Limited Systems and methods for improving protein and milk production of dairy herds
US20040191776A1 (en) * 2003-03-21 2004-09-30 Pei-Jer Chen Method for genotyping and quantifying Hepatitis B Virus
GB2399776A (en) * 2003-03-24 2004-09-29 Pa Knowledge Ltd Cyclical heating and cooling device and associated methods
WO2004092412A2 (en) 2003-03-31 2004-10-28 Roche Diagnostics Gmbh Compositions and methods for detecting certain flaviviruses, including members of the japanese encephalitis virus serogroup
EP1613771B1 (en) 2003-04-04 2012-03-21 Roche Diagnostics GmbH Improved system for multi color real time pcr
CA2463719A1 (en) * 2003-04-05 2004-10-05 F. Hoffmann-La Roche Ag Nucleotide analogs with six membered rings
WO2004094986A2 (en) * 2003-04-16 2004-11-04 Handylab, Inc. System and method for electrochemical detection of biological compounds
US20060177824A1 (en) * 2003-04-29 2006-08-10 Procop Gary W Salmonella detection identification
US7148043B2 (en) 2003-05-08 2006-12-12 Bio-Rad Laboratories, Inc. Systems and methods for fluorescence detection with a movable detection module
JP4705035B2 (ja) 2003-05-23 2011-06-22 バイオ−ラッド ラボラトリーズ，インコーポレイティド 反応培地の空間配列に対し局部化した温度制御
US20070059701A1 (en) * 2003-06-30 2007-03-15 Astrazeneca Ab Fluorescence assays for nucleic acid polymerase activity
EP1493822A1 (de) * 2003-07-02 2005-01-05 Labor Becker, Olgemoeller & Kollegen GbR Detektionsverfahren für Nucleinsäuren mit interner Amplifikationskontrolle
GB0317343D0 (en) * 2003-07-24 2003-08-27 Medical Biosystems Ltd Polynucleotide sequencing
EP1502958A1 (en) * 2003-08-01 2005-02-02 Roche Diagnostics GmbH New detection format for hot start real time polymerase chain reaction
US20050053950A1 (en) * 2003-09-08 2005-03-10 Enrique Zudaire Ubani Protocol and software for multiplex real-time PCR quantification based on the different melting temperatures of amplicons
US7788039B2 (en) 2003-09-25 2010-08-31 Roche Molecular Systems, Inc. Quantitation of nucleic acids using growth curves
WO2005043168A2 (en) * 2003-11-04 2005-05-12 Roche Diagnostics Gmbh Method for distinguishing aml-specific flt3 length mutations from tkd mutations
US20070128607A1 (en) * 2003-11-04 2007-06-07 Martin Dugas Method for distinguishing aml subtypes with different gene dosages
EP1682902A2 (en) * 2003-11-04 2006-07-26 Roche Diagnostics GmbH Method for distinguishing mll-ptd-positive aml from other aml subtypes
EP1533618A1 (en) * 2003-11-04 2005-05-25 Ludwig-Maximilians-Universität München Method for distinguishing prognostically definable AML
EP1530046A1 (en) * 2003-11-04 2005-05-11 Ludwig-Maximilians-Universität München Method for distinguishing AML subtypes with aberrant and prognostically intermediate karyotypes
EP1682901A2 (en) * 2003-11-04 2006-07-26 Roche Diagnostics GmbH Method for distinguishing leukemia subtypes
EP1682898A2 (en) * 2003-11-04 2006-07-26 Roche Diagnostics GmbH Method for distinguishing aml subtypes with recurring genetic aberrations
EP1682897A2 (en) * 2003-11-04 2006-07-26 Roche Diagnostics GmbH Method for distinguishing immunologically defined all subtypes
WO2005045435A2 (en) * 2003-11-04 2005-05-19 Roche Diagnostics Gmbh METHOD FOR DISTINGUISHING T(11q23)/MLL-POSITIVE LEUKEMIAS FROM T(11q23)MLL NEGATIVE LEUKEMIAS
US20070212688A1 (en) * 2003-11-04 2007-09-13 Martin Dugas Method For Distinguishing Cbf-Positive Aml Subtypes From Cbf-Negative Aml Subtypes
US7691570B2 (en) * 2003-11-06 2010-04-06 Dual Juerg Chemical analysis using dynamic viscometry
US7427475B2 (en) * 2003-11-18 2008-09-23 Mayo Foundation For Medical Education And Research Detection of group B streptococcus
WO2005051967A2 (en) * 2003-11-19 2005-06-09 Allelogic Biosciences Corp. Oligonucleotides labeled with a plurality of fluorophores
US7582452B2 (en) * 2003-11-24 2009-09-01 Life Genetics Lab, Llc Assay for species sources
CA2494571C (en) 2003-12-02 2010-02-09 F.Hoffmann-La Roche Ag Oligonucleotides containing molecular rods
WO2005054502A1 (en) 2003-12-02 2005-06-16 Roche Diagnostics Gmbh Improved method for bisulfite treatment
US20050130213A1 (en) * 2003-12-10 2005-06-16 Tom Morrison Selective ligation and amplification assay
US8697433B2 (en) * 2003-12-10 2014-04-15 Samsung Electronics Co., Ltd. Polymerase chain reaction (PCR) module and multiple PCR system using the same
EP1716257B8 (en) 2004-02-10 2012-11-07 Roche Diagnostics GmbH New primers and probes for the detection of parvovirus b19
US20050186606A1 (en) * 2004-02-11 2005-08-25 Schroeder Benjamin G. Methods and compositions for detecting nucleic acids
EP1718770B1 (en) 2004-02-19 2011-05-25 The Governors of the University of Alberta Leptin promoter polymorphisms and uses thereof
WO2005085474A2 (en) * 2004-03-02 2005-09-15 Clontech Laboratories Inc. Fluorescent energy transfer labeled nucleic acid substrates and methods of use thereof
US8304192B2 (en) 2004-03-05 2012-11-06 University Of Toledo Methods and compositions for assessing nucleic acids and alleles
CA2559171A1 (en) 2004-03-12 2005-09-29 Biotrove, Inc. Nanoliter array loading
JP4972541B2 (ja) 2004-04-01 2012-07-11 バイオ−ラッド ラボラトリーズ インコーポレーティッド 標識されたプローブおよび３’→５’エキソヌクレアーゼ活性を用いた定量的増幅方法
EP1745157A4 (en) * 2004-04-12 2008-06-11 Ohio Med College METHOD AND COMPOSITIONS FOR TESTING ANALYTES
EP1745120A4 (en) * 2004-04-16 2010-04-14 Spartan Bioscience Inc SYSTEM FOR RAPID AMPLIFICATION AND DETECTION OF NUCLEIC ACIDS
US9657347B2 (en) 2004-04-20 2017-05-23 University of Utah Research Foundation and BioFire Defense, LLC Nucleic acid melting analysis with saturation dyes
US7387887B2 (en) * 2004-04-20 2008-06-17 University Of Utah Research Foundation Nucleic acid melting analysis with saturation dyes
US7939251B2 (en) 2004-05-06 2011-05-10 Roche Molecular Systems, Inc. SENP1 as a marker for cancer
US20050250112A1 (en) * 2004-05-07 2005-11-10 Padmabandu Gothami A Nucleic acids, compositions, methods, and kits for detecting mycoplasma and acholeplasma species
NZ582558A (en) * 2004-05-13 2012-06-29 Anita Goel Nano-PCR: Methods and devices for nucleic acid amplification and detection
DE102004025538A1 (de) * 2004-05-25 2005-12-22 Advalytix Ag Temperierverfahren und -vorrichtung für die Temperaturbehandlung kleiner Flüssigkeitsmengen
EP2290071B1 (en) * 2004-05-28 2014-12-31 Asuragen, Inc. Methods and compositions involving microRNA
EP1605060A1 (en) * 2004-06-10 2005-12-14 Biodynamics S.R.L. Methods and reaction mixture reagent for increasing the specificity and fidelity of polymerase reactions
CA2566336C (en) * 2004-06-25 2014-05-13 E.I. Du Pont De Nemours And Company Dna sequences for the detection of and differentation amongst pathogenic e.coli
EP1610170A1 (en) * 2004-06-25 2005-12-28 Sony Deutschland GmbH A method of applying a particle film to create a surface having light-diffusive and/or reduced glare properties
GB0416944D0 (en) 2004-07-29 2004-09-01 Lumora Ltd Method for determining the amount of template nucleic acid present in a sample
TWI349704B (en) * 2004-08-10 2011-10-01 Food And Drug Administration Dept Of Health A method for rapidly detecting quinolone-resistant salmonella spp. and the probes and primers utilized therein
US7361469B2 (en) * 2004-08-13 2008-04-22 Stratagene California Dual labeled fluorescent probes
EP1632578A1 (en) * 2004-09-03 2006-03-08 Roche Diagnostics GmbH DNA decontamination method
CA2579349A1 (en) 2004-09-07 2006-03-16 Telethon Institute For Child Health Research Agents for treatment or prevention of an allergic disorder
EP1634965B1 (en) 2004-09-09 2010-01-20 Roche Diagnostics GmbH Real time PCR with the addition of pyrophosphatase
US20060051796A1 (en) * 2004-09-09 2006-03-09 Inga Boell Real time PCR with the addition of pyrophosphatase
CN101068937A (zh) * 2004-09-20 2007-11-07 匹兹堡大学 多重模式复合反应测序方法
ATE494392T1 (de) 2004-09-21 2011-01-15 Life Technologies Corp Zweifarbige echtzeit/endpunkt-quantifizierung von mikro-rnas (mirnas)
WO2006042136A2 (en) * 2004-10-07 2006-04-20 Chronix Biomedical Detection of nucleic acids to assess risk for creutzfeldt-jakob disease
US20060078894A1 (en) * 2004-10-12 2006-04-13 Winkler Matthew M Methods and compositions for analyzing nucleic acids
JP5543065B2 (ja) * 2004-10-18 2014-07-09 ブランデイズ ユニバーシティー 核酸増幅のためのプライマー、プローブおよび方法
US20060194222A1 (en) * 2004-10-20 2006-08-31 Stratagene California Triplex probe compositions and methods for polynucleotide detection
EP2316927B1 (en) 2004-10-21 2014-11-05 Helmholtz Zentrum München Deutsches Forschungszentrum für Gesundheit und Umwelt (GmbH) Kaspp (lrrk2) gene, its production and use for the detection and treatment of neurodegenerative disorders
CN101068932B (zh) * 2004-10-27 2013-02-13 塞弗德公司 封闭系统多阶段核酸扩增反应
WO2006048262A2 (en) * 2004-11-04 2006-05-11 Roche Diagnostics Gmbh Classification of acute myeloid leukemia
AU2005333165B2 (en) 2004-11-12 2012-07-19 Asuragen, Inc. Methods and compositions involving miRNA and miRNA inhibitor molecules
WO2008073922A2 (en) * 2006-12-08 2008-06-19 Asuragen, Inc. Functions and targets of let-7 micro rnas
CA2594223C (en) * 2005-01-03 2016-03-15 The Government Of The United States Of America, As Represented By The Secretary Of The Department Of Health And Human Services, Centers For Disease Control And Prevention Primer for nucleic acid detection
EP2208796B1 (en) 2005-01-06 2014-06-18 Applied Biosystems, LLC Polypeptides having nucleic acid binding activity and compositions and methods for nucleic acid amplification
US7315376B2 (en) * 2005-01-07 2008-01-01 Advanced Molecular Systems, Llc Fluorescence detection system
ATE359500T1 (de) 2005-01-18 2007-05-15 Hoffmann La Roche Fluoreszenzabbildung mittels telezentrizität
EP1681555B1 (en) 2005-01-18 2007-03-21 Roche Diagnostics GmbH Imaging of fluorescence signals using telecentric excitation and imaging optics
US8470608B2 (en) * 2008-05-20 2013-06-25 Rapid Pathogen Screening, Inc Combined visual/fluorescence analyte detection test
US8669052B2 (en) * 2008-06-10 2014-03-11 Rapid Pathogen Screening, Inc. Lateral flow nucleic acid detector
US20060246423A1 (en) * 2005-02-10 2006-11-02 Adelson Martin E Method and kit for the collection and maintenance of the detectability of a plurality of microbiological species in a single gynecological sample
US7393665B2 (en) 2005-02-10 2008-07-01 Population Genetics Technologies Ltd Methods and compositions for tagging and identifying polynucleotides
US20060178838A1 (en) * 2005-02-10 2006-08-10 Adelson Martin E Method of receiving and handling a plurality of clinical samples for reporting a sum of diagnostic results for each sample
US9464310B2 (en) * 2005-02-10 2016-10-11 Medical Diagnostic Laboratories, Llc Integrated method for collection and maintenance of detectability of a plurality of microbiological agents in a single clinical sample and for handling a plurality of samples for reporting a sum of diagnostic results for each sample
US20060188891A1 (en) * 2005-02-23 2006-08-24 Bickmore William D Jr Methods and apparatus for controlling DNA amplification
JP4398886B2 (ja) * 2005-03-07 2010-01-13 ソニー株式会社 通信端末装置、通信システム、通信方法、およびプログラム
US7759469B2 (en) 2005-03-10 2010-07-20 Roche Diagnostics Operations, Inc. Labeling reagent
EP1930730B1 (en) 2005-03-10 2019-08-14 Gen-Probe Incorporated Systems and methods to perform assays for detecting or quantifying analytes
EP1700922B1 (en) 2005-03-10 2016-08-24 Roche Diagnostics GmbH 3-Substituted 5-Nitroindole derivatives and labeled oligonucleotide probes containing them
CA2967430C (en) 2005-03-10 2018-05-08 Gen-Probe Incorporated Systems and methods to perform assays for detecting or quantifying analytes within samples
US7680604B2 (en) 2005-03-11 2010-03-16 Roche Molecular Systems, Inc. PCR elbow determination by rotational transform after zero slope alignment
US7601498B2 (en) * 2005-03-17 2009-10-13 Biotium, Inc. Methods of using dyes in association with nucleic acid staining or detection and associated technology
US7776567B2 (en) * 2005-03-17 2010-08-17 Biotium, Inc. Dimeric and trimeric nucleic acid dyes, and associated systems and methods
US7456281B2 (en) * 2005-04-20 2008-11-25 Idaho Technology, Inc. Nucleic acid melting analysis with saturation dyes
EP1877585A2 (en) * 2005-04-28 2008-01-16 Merck & Co., Inc. Real-time hpv pcr assays
US20070015180A1 (en) * 2005-05-02 2007-01-18 Stratagene California Oligonucleotide probe/primer compositions and methods for polynucleotide detection
US7569695B2 (en) * 2005-05-24 2009-08-04 Enzo Life Sciences, Inc. Dyes for the detection or quantification of desirable target molecules
US7737281B2 (en) * 2005-05-24 2010-06-15 Enzo Life Sciences, Inc. C/O Enzo Biochem, Inc. Purine based fluorescent dyes
US8362250B2 (en) 2005-05-24 2013-01-29 Enzo Biochem, Inc. Fluorescent dyes and compounds, methods and kits useful for identifying specific organelles and regions in cells of interest
US8357801B2 (en) 2005-05-24 2013-01-22 Enzo Life Sciences, Inc. Labeling of target molecules, identification of organelles and other applications, novel compositions, methods and kits
JP4879975B2 (ja) * 2005-05-31 2012-02-22 アプライド バイオシステムズ リミテッド ライアビリティー カンパニー 短い核酸のマルチプレックス増幅
US20060281099A1 (en) * 2005-06-14 2006-12-14 Bio-Rad Laboratories, Inc. Q-melt for polymorphism detection
EP2620510B2 (en) 2005-06-15 2020-02-19 Complete Genomics Inc. Single molecule arrays for genetic and chemical analysis
US20060286590A1 (en) * 2005-06-20 2006-12-21 Applera Corporation. Cis-regulatory modules
US20060292578A1 (en) 2005-06-28 2006-12-28 Weidong Zheng DNA polymerase blends and mutant DNA polymerases
GB0514889D0 (en) 2005-07-20 2005-08-24 Lgc Ltd Oligonucleotides
ES2494922T3 (es) * 2005-09-01 2014-09-16 Ausdiagnostics Pty Ltd. Métodos para la amplificación, cuantificación e identificación de ácidos nucleicos
US7569367B2 (en) 2005-09-07 2009-08-04 Roche Diagnostics Operations, Inc. Nucleic acid preparation from whole blood for use in diagnosis of transmissible spongiform encephalopathy
US20070059713A1 (en) 2005-09-09 2007-03-15 Lee Jun E SSB-DNA polymerase fusion proteins
US7991558B2 (en) 2005-09-29 2011-08-02 Roche Molecular Systems, Inc. Systems and methods for determining real-time PCR cycle thresholds using cluster analysis
US7991561B2 (en) 2005-09-29 2011-08-02 Roche Molecular Systems, Inc. Ct determination by cluster analysis with variable cluster endpoint
WO2007041201A2 (en) 2005-10-03 2007-04-12 Applera Corporation Compositions, methods, and kits for amplifying nucleic acids
EP2546360A1 (en) 2005-10-07 2013-01-16 Callida Genomics, Inc. Self-assembled single molecule arrays and uses thereof
WO2007047606A2 (en) * 2005-10-17 2007-04-26 Board Of Supervisors Of Louisiana State University And Agricultural And Mechanical College Electrokinetic thermal cycler and reactor
US7781165B2 (en) 2005-10-19 2010-08-24 Roche Diagnostics Operations, Inc. Benzimidazolium compounds and salts of benzimidazolium compounds for nucleic acid amplification
WO2007120208A2 (en) * 2005-11-14 2007-10-25 President And Fellows Of Harvard College Nanogrid rolling circle dna sequencing
DK2463388T3 (en) 2005-11-29 2018-02-26 Cambridge Entpr Ltd Markers for breast cancer
EP1798291B1 (en) * 2005-12-13 2011-05-18 Institut Curie Methods and compositions for assaying mutations and/or large scale alterations in nucleic acids and their uses in diagnosis of genetic diseases and cancers
JP5374679B2 (ja) * 2005-12-14 2013-12-25 エピゲノミクス アーゲー 重亜硫酸処理のための新規方法
EP1798650A1 (en) * 2005-12-19 2007-06-20 Roche Diagnostics GmbH Analytical method and instrument
EP1798542A1 (en) * 2005-12-19 2007-06-20 Roche Diagnostics GmbH Analytical method and instrument
US7668663B2 (en) 2005-12-20 2010-02-23 Roche Molecular Systems, Inc. Levenberg-Marquardt outlier spike removal method
US7844403B2 (en) 2005-12-20 2010-11-30 Roche Molecular Systems, Inc. Temperature step correction with double sigmoid Levenberg-Marquardt and robust linear regression
US7991562B2 (en) 2005-12-20 2011-08-02 Roche Molecular Systems, Inc. PCR elbow determination using curvature analysis of a double sigmoid
US7680868B2 (en) 2005-12-20 2010-03-16 Roche Molecular Systems, Inc. PCR elbow determination by use of a double sigmoid function curve fit with the Levenburg-Marquardt algorithm and normalization
US7981606B2 (en) 2005-12-21 2011-07-19 Roche Molecular Systems, Inc. Control for nucleic acid testing
EP2025762A3 (en) 2006-01-17 2009-09-30 Health Research Inc. Heteroduplex tracking assay
US7785786B2 (en) * 2006-01-23 2010-08-31 Quest Diagnostics Investments Incorporated Methods for detecting nucleic acids using multiple signals
US20090305238A1 (en) * 2006-01-23 2009-12-10 Applera Corporation Microarray Microcard
WO2007092538A2 (en) 2006-02-07 2007-08-16 President And Fellows Of Harvard College Methods for making nucleotide probes for sequencing and synthesis
GB0603190D0 (en) * 2006-02-16 2006-03-29 Enigma Diagnostics Ltd Detection system
EP1989324B1 (en) * 2006-02-27 2011-05-18 Roche Diagnostics GmbH Pcr hot start by magnesium sequestration
US20110143344A1 (en) * 2006-03-01 2011-06-16 The Washington University Genetic polymorphisms and substance dependence
BRPI0708439A2 (pt) * 2006-03-01 2011-05-31 Perlegen Sciences Inc marcadores para vìcio
RU2394915C2 (ru) * 2006-03-24 2010-07-20 Александр Борисович Четверин Бесконтактные способы обнаружения молекулярных колоний, наборы реагентов и устройство для их осуществления
US8778637B2 (en) 2006-03-28 2014-07-15 Canon U.S. Life Sciences, Inc. Method and apparatus for applying continuous flow and uniform temperature to generate thermal melting curves in a microfluidic device
US7455844B2 (en) 2006-03-29 2008-11-25 Merial Limited Vaccine against streptococci
EP2002013A4 (en) 2006-04-04 2010-05-05 Labonnet Ltd ASSESSMENT OF REACTION KINETICS COMPATIBILITY BETWEEN POLYMERASE CHAIN REACTIONS
US20070238108A1 (en) * 2006-04-07 2007-10-11 Agilent Technologies, Inc. Validation of comparative genomic hybridization
US20070238093A1 (en) * 2006-04-11 2007-10-11 Espy Mark J Detection of influenza A virus
US20070238095A1 (en) * 2006-04-11 2007-10-11 Mayo Foundation For Medical Education And Research , A Minnesota Corporation Detection of Influenza A Virus
CN101479605A (zh) 2006-04-21 2009-07-08 纳诺拜希姆公司 用于药物发现的单分子平台：用于包括抗癌和抗病毒剂的发现的药物发现的方法和装置
US8349556B2 (en) 2006-04-28 2013-01-08 Igor Kutyavin Use of base-modified deoxynucleoside triphosphates to improve nucleic acid detection
US8900828B2 (en) 2006-05-01 2014-12-02 Cepheid Methods and apparatus for sequential amplification reactions
US8232091B2 (en) * 2006-05-17 2012-07-31 California Institute Of Technology Thermal cycling system
EP2027251A4 (en) * 2006-05-17 2010-05-05 California Inst Of Techn Thermal cycling system
US9133504B2 (en) * 2006-06-05 2015-09-15 California Institute Of Technology Real time microarrays
US11001881B2 (en) 2006-08-24 2021-05-11 California Institute Of Technology Methods for detecting analytes
US8637436B2 (en) 2006-08-24 2014-01-28 California Institute Of Technology Integrated semiconductor bioarray
US8119352B2 (en) * 2006-06-20 2012-02-21 Cepheld Multi-stage amplification reactions by control of sequence replication times
US7629124B2 (en) 2006-06-30 2009-12-08 Canon U.S. Life Sciences, Inc. Real-time PCR in micro-channels
US8048626B2 (en) 2006-07-28 2011-11-01 California Institute Of Technology Multiplex Q-PCR arrays
US11525156B2 (en) 2006-07-28 2022-12-13 California Institute Of Technology Multiplex Q-PCR arrays
WO2008021010A2 (en) * 2006-08-07 2008-02-21 Stratagene California Methods for real-time quantitative pcr
US11560588B2 (en) 2006-08-24 2023-01-24 California Institute Of Technology Multiplex Q-PCR arrays
US9481912B2 (en) 2006-09-12 2016-11-01 Longhorn Vaccines And Diagnostics, Llc Compositions and methods for detecting and identifying nucleic acid sequences in biological samples
US8080645B2 (en) 2007-10-01 2011-12-20 Longhorn Vaccines & Diagnostics Llc Biological specimen collection/transport compositions and methods
US8097419B2 (en) 2006-09-12 2012-01-17 Longhorn Vaccines & Diagnostics Llc Compositions and method for rapid, real-time detection of influenza A virus (H1N1) swine 2009
EP1900824A1 (en) * 2006-09-14 2008-03-19 Deutsches Krebsforschungszentrum Stiftung Des Öffentlichen Rechts Gene expression signature for the prognosis, diagnosis and therapy of prostate cancer and uses thereof
CA2663962A1 (en) * 2006-09-19 2008-03-27 Asuragen, Inc. Mir-15, mir-26, mir-31,mir-145, mir-147, mir-188, mir-215, mir-216, mir-331, mmu-mir-292-3p regulated genes and pathways as targets for therapeutic intervention
US20100297026A1 (en) * 2006-11-02 2010-11-25 Doyle Gerald V Imaging of Activated Vascular Endothelium Using Immunomagnetic MRI Contrast Agents
US9114398B2 (en) 2006-11-29 2015-08-25 Canon U.S. Life Sciences, Inc. Device and method for digital multiplex PCR assays
US20080131878A1 (en) * 2006-12-05 2008-06-05 Asuragen, Inc. Compositions and Methods for the Detection of Small RNA
CA2671194A1 (en) * 2006-12-08 2008-06-19 Asuragen, Inc. Mir-20 regulated genes and pathways as targets for therapeutic intervention
CN101622349A (zh) * 2006-12-08 2010-01-06 奥斯瑞根公司 作为治疗性干预靶标的miR-21调节的基因和途径
AU2007333107A1 (en) * 2006-12-08 2008-06-19 Asuragen, Inc. miR-21 regulated genes and pathways as targets for therapeutic intervention
KR101433208B1 (ko) 2006-12-13 2014-08-22 루미넥스 코포레이션 실시간으로 ｐｃｒ의 다중 분석을 위한 시스템과 방법
ES2701872T3 (es) 2006-12-21 2019-02-26 Agriculture Victoria Serv Pty Métodos y reactivos de selección artificial
US8535888B2 (en) 2006-12-29 2013-09-17 Mayo Foundation For Medical Education And Research Compositions and methods for detecting methicillin-resistant S. aureus
CA2671270A1 (en) * 2006-12-29 2008-07-17 Asuragen, Inc. Mir-16 regulated genes and pathways as targets for therapeutic intervention
EP2126114B1 (en) * 2007-02-01 2016-09-28 Abacus Diagnostica OY Method for detection of presence of target polynucleotide in samples
AU2008210279B2 (en) 2007-02-02 2013-10-31 Genera Biosystems Limited Generation of nucleic acid molecules
US20100190167A1 (en) * 2007-02-14 2010-07-29 Genisphere Inc. Methods, Reagents and Kits for Detection of Nucleic Acid Molecules
JP4942508B2 (ja) * 2007-02-20 2012-05-30 アークレイ株式会社 ａｂｌ遺伝子変異の検出用プローブおよびその用途
US9475051B2 (en) * 2007-02-27 2016-10-25 Sony Corporation Nucleic acid amplifier
GB0703997D0 (en) * 2007-03-01 2007-04-11 Oxitec Ltd Methods for detecting nucleic sequences
GB0703996D0 (en) * 2007-03-01 2007-04-11 Oxitec Ltd Nucleic acid detection
CN103952470B (zh) * 2007-03-28 2017-11-10 信号诊断公司 高解析度分析核酸以检测序列变异的系统和方法
JP5191041B2 (ja) 2007-04-05 2013-04-24 エフ．ホフマン−ラ ロシュ アーゲー 急速ワンステップｒｔ−ｐｃｒ
EP2150625B1 (en) 2007-04-19 2013-03-20 Molecular Detection Inc. Methods, compositions and kits for detection and analysis of antibiotic-resistant bacteria
US20090232893A1 (en) * 2007-05-22 2009-09-17 Bader Andreas G miR-143 REGULATED GENES AND PATHWAYS AS TARGETS FOR THERAPEUTIC INTERVENTION
CN101801419A (zh) * 2007-06-08 2010-08-11 米尔纳疗法公司 作为治疗干预的靶标的miR-34调控的基因和路径
DE102007031137A1 (de) * 2007-06-13 2008-12-18 Attomol Gmbh Molekulare Diagnostika Verfahren und Sonden/Primärsystem zum "real time" Nachweis eines Nukleinsäuretargets
ES2538100T3 (es) * 2007-06-13 2015-06-17 Attomol Gmbh Molekulare Diagnostika Procedimiento para la realización y evaluación de ensayos de mezclado y medición para la medición de cinéticas de reacción así como de concentraciones y afinidades de analitos en formato múltiplex
WO2008157801A2 (en) 2007-06-21 2008-12-24 Gen-Probe Incorporated Instrument and receptacles for performing processes
US7635566B2 (en) 2007-06-29 2009-12-22 Population Genetics Technologies Ltd. Methods and compositions for isolating nucleic acid sequence variants
US20090023138A1 (en) * 2007-07-17 2009-01-22 Zila Biotechnology, Inc. Oral cancer markers and their detection
US20090055243A1 (en) 2007-08-21 2009-02-26 Jayson Lee Lusk Systems and methods for predicting a livestock marketing method
US11041215B2 (en) 2007-08-24 2021-06-22 Longhorn Vaccines And Diagnostics, Llc PCR ready compositions and methods for detecting and identifying nucleic acid sequences
US8386184B2 (en) 2007-08-28 2013-02-26 Becton, Dickinson And Company Systems and methods for determining an amount of starting reagent using the polymerase chain reaction
US8380457B2 (en) 2007-08-29 2013-02-19 Canon U.S. Life Sciences, Inc. Microfluidic devices with integrated resistive heater electrodes including systems and methods for controlling and measuring the temperatures of such heater electrodes
DE102007041864B4 (de) 2007-09-04 2012-05-03 Sirs-Lab Gmbh Verfahren zum Nachweis von Bakterien und Pilzen
EP2198050A1 (en) * 2007-09-14 2010-06-23 Asuragen, INC. Micrornas differentially expressed in cervical cancer and uses thereof
US20090119020A1 (en) 2007-09-25 2009-05-07 Roche Molecular Systems, Inc. Pcr elbow determination using quadratic test for curvature analysis of a double sigmoid
CN101809170B (zh) 2007-09-28 2014-08-20 3M创新有限公司 用于检测核酸的双寡核苷酸方法
US20090186015A1 (en) * 2007-10-18 2009-07-23 Latham Gary J Micrornas differentially expressed in lung diseases and uses thereof
FR2922897B1 (fr) * 2007-10-25 2009-11-27 Bio Rad Pasteur Mesure d'une population d'acides nucleiques,en particulier par pcr en temps reel.
US8145433B2 (en) * 2007-10-25 2012-03-27 Canon U.S. Life Sciences, Inc. High-resolution melting analysis
US9382585B2 (en) * 2007-10-30 2016-07-05 Complete Genomics, Inc. Apparatus for high throughput sequencing of nucleic acids
US20090181391A1 (en) * 2007-11-01 2009-07-16 Zymo Research Corporation Methods for analysis of dna methylation percentage
US20090137058A1 (en) * 2007-11-28 2009-05-28 General Electric Company Chemical and biological detection method and device based on measurements of fluorescence and reflectivity
US8071562B2 (en) * 2007-12-01 2011-12-06 Mirna Therapeutics, Inc. MiR-124 regulated genes and pathways as targets for therapeutic intervention
US9551026B2 (en) 2007-12-03 2017-01-24 Complete Genomincs, Inc. Method for nucleic acid detection using voltage enhancement
US20090162863A1 (en) * 2007-12-13 2009-06-25 Hitachi High-Technologies Corporation Nucleic acid detection probe
WO2009086156A2 (en) * 2007-12-21 2009-07-09 Asuragen, Inc. Mir-10 regulated genes and pathways as targets for therapeutic intervention
US8364415B2 (en) * 2007-12-26 2013-01-29 Arkray, Inc. Melting curve analyzing method and melting curve analyzing device
US8306754B2 (en) * 2007-12-26 2012-11-06 Arkray, Inc. Nucleic acid amplification determining method and nucleic acid amplification determining device
DE102007063102B4 (de) 2007-12-28 2022-02-10 Robert Bosch Gmbh Verfahren zur Erfassung eines periodisch pulsierenden Betriebsparameters
WO2009092564A2 (en) * 2008-01-23 2009-07-30 Roche Diagnostics Gmbh Integrated instrument performing synthesis and amplification
WO2009100430A2 (en) * 2008-02-08 2009-08-13 Asuragen, Inc miRNAs DIFFERENTIALLY EXPRESSED IN LYMPH NODES FROM CANCER PATIENTS
WO2009100215A2 (en) * 2008-02-08 2009-08-13 Mayo Foundation For Medical Education And Research Detection of clostridium difficile
WO2009103003A2 (en) * 2008-02-15 2009-08-20 Bio-Rad Laboratories, Inc. Scanning fluorescent reader with diffuser system
US8153374B2 (en) * 2008-03-03 2012-04-10 Heatflow Technologies, Inc. Heat flow polymerase chain reaction methods
EP2291541A4 (en) * 2008-03-05 2011-06-22 Clean Earth Tech Llc MULTIPLEX PCR COUPLED FLOW CYTOMETRIC TEST WITH PNA-MARKED BEADS FOR THE SIMULTANEOUS DETECTION OF MULTIPLE BIOLOGICAL SUBSTANCES
CA2658520C (en) * 2008-03-19 2016-11-08 F. Hoffmann-La Roche Ag Nucleic acid amplification in the presence of modified randomers
EP2271757A2 (en) * 2008-03-26 2011-01-12 Asuragen, INC. Compositions and methods related to mir-16 and therapy of prostate cancer
WO2009126726A1 (en) * 2008-04-08 2009-10-15 Asuragen, Inc Methods and compositions for diagnosing and modulating human papillomavirus (hpv)
ATE537272T1 (de) * 2008-04-08 2011-12-15 Hoffmann La Roche Detektion von pcr-produkten in der gelelektrophorese
EP2108699B1 (en) 2008-04-08 2014-06-25 F.Hoffmann-La Roche Ag Analytical processing and detection device
EP2990487A1 (en) * 2008-05-08 2016-03-02 Asuragen, INC. Compositions and methods related to mirna modulation of neovascularization or angiogenesis
US8962260B2 (en) 2008-05-20 2015-02-24 Rapid Pathogen Screening, Inc. Method and device for combined detection of viral and bacterial infections
US9068981B2 (en) 2009-12-04 2015-06-30 Rapid Pathogen Screening, Inc. Lateral flow assays with time delayed components
US8609433B2 (en) 2009-12-04 2013-12-17 Rapid Pathogen Screening, Inc. Multiplanar lateral flow assay with sample compressor
US20130196310A1 (en) 2008-05-20 2013-08-01 Rapid Pathogen Screening, Inc. Method and Device for Combined Detection of Viral and Bacterial Infections
US8815609B2 (en) 2008-05-20 2014-08-26 Rapid Pathogen Screening, Inc. Multiplanar lateral flow assay with diverting zone
EP2130929B1 (en) 2008-06-06 2013-10-09 F. Hoffmann-La Roche AG Internally controlled multiplex detection and quantification of microbial nucleic acids
US20110086359A1 (en) 2008-06-10 2011-04-14 Rapid Pathogen Screening, Inc. Lateral flow assays
EP2138588A1 (en) * 2008-06-23 2009-12-30 Koninklijke Philips Electronics N.V. Melting curve measurement during amplification
US8097412B2 (en) 2008-07-12 2012-01-17 Biodiagnostics, Inc. DNA-based test for detection of annual and intermediate ryegrass
DE102008040513B4 (de) * 2008-07-17 2010-08-26 BAM Bundesanstalt für Materialforschung und -prüfung Verwendung einer langwellig emittierenden Cyaninverbindung als NIR-Fluoreszenzstandard und Kit zur Kalibrierung von Photolumineszenzmesssystemen
EP2148187A1 (de) 2008-07-25 2010-01-27 Roche Diagnostics GmbH Anregungs- und Abbildungsoptik für die Fluoreszenzdetektion
CA2638458A1 (en) * 2008-07-31 2010-01-31 Spartan Bioscience Inc. Thermal recycling by positioning relative to fixed-temperature source
GB0909333D0 (en) 2009-06-01 2009-07-15 Fu Guoliang Multiplex amplification and detection
WO2010012464A1 (en) 2008-08-01 2010-02-04 Roche Diagnostics Gmbh Improved lysis and reverse transcription for mrna quantification
US20100055733A1 (en) * 2008-09-04 2010-03-04 Lutolf Matthias P Manufacture and uses of reactive microcontact printing of biomolecules on soft hydrogels
US9334281B2 (en) * 2008-09-08 2016-05-10 Enzo Life Sciences, Inc. Fluorochromes for organelle tracing and multi-color imaging
US9250249B2 (en) 2008-09-08 2016-02-02 Enzo Biochem, Inc. Autophagy and phospholipidosis pathway assays
US7910720B2 (en) 2008-09-09 2011-03-22 Roche Diagnostics Operations, Inc. Polyanion for improved nucleic acid amplification
WO2010030686A1 (en) * 2008-09-09 2010-03-18 Bio-Rad Laboratories, Inc. Multi-stage, regression-based pcr analysis system
US8219324B2 (en) 2008-09-12 2012-07-10 Roche Molecular Systems, Inc. Real-time PCR elbow calling by equation-less algorithm
US9540686B2 (en) * 2008-09-18 2017-01-10 Canon U.S. Life Sciences, Inc. Systems and methods for the amplification of DNA
CA2682439A1 (en) 2008-10-17 2010-04-17 F. Hoffmann-La Roche Ag Cell monitoring and molecular analysis
US8170804B2 (en) * 2008-10-17 2012-05-01 Roche Molecular Systems, Inc. Determination of melting temperatures of DNA
US20100301398A1 (en) 2009-05-29 2010-12-02 Ion Torrent Systems Incorporated Methods and apparatus for measuring analytes
US20100112630A1 (en) * 2008-11-03 2010-05-06 Scott Martell Boyette Methods for measuring microbiological content in aqueous media
US8481302B2 (en) * 2008-11-03 2013-07-09 General Electric Company Total bacteria monitoring system
US20100119454A1 (en) * 2008-11-03 2010-05-13 Ping Shen Use of the conserved Drosophila NPFR1 system for uncovering interacting genes and pathways important in nociception and stress response
US8748103B2 (en) 2008-11-07 2014-06-10 Sequenta, Inc. Monitoring health and disease status using clonotype profiles
GB2483810B (en) 2008-11-07 2012-09-05 Sequenta Inc Methods for correlating clonotypes with diseases in a population
US9506119B2 (en) 2008-11-07 2016-11-29 Adaptive Biotechnologies Corp. Method of sequence determination using sequence tags
US9365901B2 (en) 2008-11-07 2016-06-14 Adaptive Biotechnologies Corp. Monitoring immunoglobulin heavy chain evolution in B-cell acute lymphoblastic leukemia
US8691510B2 (en) 2008-11-07 2014-04-08 Sequenta, Inc. Sequence analysis of complex amplicons
US8628927B2 (en) 2008-11-07 2014-01-14 Sequenta, Inc. Monitoring health and disease status using clonotype profiles
US9394567B2 (en) 2008-11-07 2016-07-19 Adaptive Biotechnologies Corporation Detection and quantification of sample contamination in immune repertoire analysis
US9528160B2 (en) 2008-11-07 2016-12-27 Adaptive Biotechnolgies Corp. Rare clonotypes and uses thereof
US20100179213A1 (en) * 2008-11-11 2010-07-15 Mirna Therapeutics, Inc. Methods and Compositions Involving miRNAs In Cancer Stem Cells
US10669574B2 (en) 2008-11-18 2020-06-02 XCR Diagnostics, Inc. DNA amplification technology
WO2010067055A1 (en) 2008-12-09 2010-06-17 Oxitec Ltd. Enhanced taqman probe based amplification
US20100285478A1 (en) 2009-03-27 2010-11-11 Life Technologies Corporation Methods, Compositions, and Kits for Detecting Allelic Variants
JP2012511927A (ja) 2008-12-17 2012-05-31 ライフ テクノロジーズ コーポレーション 対立遺伝子変種を検出するための方法、組成物、およびキット
CA2688174C (en) 2008-12-19 2018-08-07 F. Hoffmann-La Roche Ag Dry composition of reaction compounds with stabilized polymerase
US20100159452A1 (en) * 2008-12-22 2010-06-24 Roche Molecular Systems, Inc. Method For Detecting a Target Nucleic Acid in a Sample
US8206929B2 (en) 2009-01-07 2012-06-26 Roche Molecular Systems, Inc. Nucleic acid amplification with allele-specific suppression of sequence variants
WO2010080910A1 (en) * 2009-01-08 2010-07-15 Bio-Rad Laboratories, Inc. Methods and compositions for improving efficiency of nucleic acids amplification reactions
SG183029A1 (en) 2009-01-13 2012-08-30 Fluidigm Corp Single-cell nucleic acid analysis
EP2387627B1 (en) 2009-01-15 2016-03-30 Adaptive Biotechnologies Corporation Adaptive immunity profiling and methods for generation of monoclonal antibodies
SG173081A1 (en) 2009-01-30 2011-08-29 Kantonsspital Aarau Ag Gene dosage analysis
EP2391452B1 (en) 2009-01-30 2015-06-17 Gen-Probe Incorporated Systems and methods for detecting a signal and applying thermal energy to a signal transmission element
JP5457222B2 (ja) 2009-02-25 2014-04-02 エフ．ホフマン−ラ ロシュ アーゲー 小型化ハイスループット核酸分析
US9347092B2 (en) * 2009-02-25 2016-05-24 Roche Molecular System, Inc. Solid support for high-throughput nucleic acid analysis
US8606527B2 (en) 2009-02-27 2013-12-10 Bio-Rad Laboratories, Inc. SNP detection by melt curve clustering
US8039215B2 (en) * 2009-03-10 2011-10-18 Roche Molecular Systems, Inc. Multiplex quantitative nucleic acid amplification and melting assay
US9542526B2 (en) * 2009-03-10 2017-01-10 Canon U.S. Life Sciences, Inc. Method and system for temperature correction in thermal melt analysis
GB0905325D0 (en) 2009-03-30 2009-05-13 Selex Sensors & Airborne Sys Detection system
EP2414504A4 (en) * 2009-04-03 2013-08-28 Illumina Inc DEVICES AND METHOD FOR HEATING BIOLOGICAL SAMPLES
WO2010116290A1 (en) 2009-04-07 2010-10-14 Koninklijke Philips Electronics N.V. Method for the detection and characterization of a toxinogenic clostridium difficile strain
US20120036593A1 (en) 2009-04-17 2012-02-09 Basf Plant Science Company Gmbh Plant Promoter Operable in Endosperm and Uses Thereof
US20120122161A1 (en) 2009-05-22 2012-05-17 Esther Musgrave-Brown Sorting Asymmetrically Tagged Nucleic Acids by Selective Primer Extension
CN102449167B (zh) 2009-05-26 2014-12-17 厦门大学 一种检测核酸序列变异的方法
US20120261274A1 (en) 2009-05-29 2012-10-18 Life Technologies Corporation Methods and apparatus for measuring analytes
US8776573B2 (en) 2009-05-29 2014-07-15 Life Technologies Corporation Methods and apparatus for measuring analytes
EP3301104B1 (en) 2009-05-29 2019-10-30 Life Technologies Corporation Scaffolded nucleic acid polymer particles and methods of making and using
US20100330571A1 (en) 2009-06-25 2010-12-30 Robins Harlan S Method of measuring adaptive immunity
EP3018218B1 (en) 2009-07-21 2018-01-24 Gen-Probe Incorporated Methods for detection of nucleic acid sequences over an extended dynamic range
WO2011022589A2 (en) * 2009-08-20 2011-02-24 Mayo Foundation For Medical Education And Research Detection of enterovirus
WO2011021102A2 (en) 2009-08-20 2011-02-24 Population Genetics Technologies Ltd Compositions and methods for intramolecular nucleic acid rearrangement
US20110059453A1 (en) * 2009-08-23 2011-03-10 Affymetrix, Inc. Poly(A) Tail Length Measurement by PCR
FI20090306A0 (fi) 2009-08-25 2009-08-25 Qret Technologies Oy Erotusvapaa määritysmenetelmä
US8219366B2 (en) 2009-08-26 2012-07-10 Roche Molecular Sytems, Inc. Determination of elbow values for PCR for parabolic shaped curves
US8987685B2 (en) * 2009-09-09 2015-03-24 Pcr Max Limited Optical system for multiple reactions
US8815515B1 (en) 2009-09-24 2014-08-26 University Of Utah Research Foundation Methods, compositions, and kits for rare allele detection
US20110111403A1 (en) * 2009-10-30 2011-05-12 Life Technologies Corporation Multi-primer assay for mycoplasma detection
WO2011053845A2 (en) 2009-10-30 2011-05-05 Illumina, Inc. Microvessels, microparticles, and methods of manufacturing and using the same
US20120245041A1 (en) 2009-11-04 2012-09-27 Sydney Brenner Base-by-base mutation screening
US9133343B2 (en) * 2009-11-30 2015-09-15 Enzo Biochem, Inc. Dyes and compositions, and processes for using same in analysis of protein aggregation and other applications
US8614071B2 (en) 2009-12-11 2013-12-24 Roche Molecular Systems, Inc. Preferential amplification of mRNA over DNA using chemically modified primers
US9238832B2 (en) 2009-12-11 2016-01-19 Roche Molecular Systems, Inc. Allele-specific amplification of nucleic acids
WO2011087789A2 (en) 2009-12-22 2011-07-21 Becton, Dickinson And Company Methods for the detection of microorganisms
US8877437B1 (en) 2009-12-23 2014-11-04 Biotium, Inc. Methods of using dyes in association with nucleic acid staining or detection
EP2524053A1 (en) * 2010-01-15 2012-11-21 Steffen Mergemeier Method for detecting more than one target in a pcr-based approach applying an unspecific dye which is not interfering with the emission of fluorophore-labeled probes
US20110177512A1 (en) * 2010-01-19 2011-07-21 Predictive Biosciences, Inc. Method for assuring amplification of an abnormal nucleic acid in a sample
US20130053253A1 (en) 2010-02-22 2013-02-28 Population Genetics Technologies Ltd Region of Interest Extraction and Normalization Methods
WO2011107887A2 (en) 2010-03-02 2011-09-09 Population Genetic Technologies Ltd. Methods for replicating polynucleotides with secondary structure
GB201004339D0 (en) 2010-03-16 2010-04-28 Enigma Diagnostics Ltd Sequence detection assay
US20130137095A1 (en) * 2010-03-29 2013-05-30 Shiro Kitano Method for identifying target base sequence
DE102010003781B4 (de) 2010-04-08 2012-08-16 Aj Innuscreen Gmbh Verfahren zum Nachweis spezifischer Nukleinsäuresequenzen
US20110250598A1 (en) 2010-04-12 2011-10-13 Ulrike Fischer Detergent free polymerases
US8774494B2 (en) 2010-04-30 2014-07-08 Complete Genomics, Inc. Method and system for accurate alignment and registration of array for DNA sequencing
US20130065790A1 (en) 2010-05-05 2013-03-14 Check-Points Holding B.V. Assays, compositions and methods for detecting drug resistant micro-organisms
WO2011139371A1 (en) 2010-05-06 2011-11-10 Sequenta, Inc. Monitoring health and disease status using clonotype profiles
GB201007868D0 (en) 2010-05-11 2010-06-23 Enigma Diagnostics Ltd Sequence detection assay
GB201007867D0 (en) 2010-05-11 2010-06-23 Enigma Diagnostics Ltd Signalling system
US8828688B2 (en) 2010-05-27 2014-09-09 Affymetrix, Inc. Multiplex amplification methods
WO2011156707A2 (en) 2010-06-11 2011-12-15 Life Technologies Corporation Alternative nucleotide flows in sequencing-by-synthesis methods
EP2585593B1 (en) 2010-06-24 2017-03-01 Population Genetics Technologies Ltd. Methods for polynucleotide library production, immortalization and region of interest extraction
US9650629B2 (en) 2010-07-07 2017-05-16 Roche Molecular Systems, Inc. Clonal pre-amplification in emulsion
CN107190056A (zh) 2010-07-29 2017-09-22 霍夫曼-拉罗奇有限公司 微生物核酸的定性和定量检测
WO2012013732A1 (en) 2010-07-29 2012-02-02 F. Hoffmann - La Roche Ag Generic pcr
WO2012013733A1 (en) 2010-07-29 2012-02-02 F. Hoffmann - La Roche Ag Generic sample preparation
ES2606688T3 (es) 2010-07-29 2017-03-27 F. Hoffmann-La Roche Ag Ácidos nucleicos de control para parámetros múltiples
JP5887041B2 (ja) 2010-08-02 2016-03-16 ウェイト、ブレント、エル． ポリメラーゼ連鎖反応のための加圧可能なカートリッジ
EP2606343A4 (en) 2010-08-18 2017-08-16 Life Technologies Corporation Chemical coating of microwell for electrochemical detection device
US9051606B2 (en) 2010-09-10 2015-06-09 Qiagen Gaithersburg, Inc. Methods and compositions for nucleic acid detection
ES2715226T3 (es) 2010-09-13 2019-06-03 Clinical Genomics Pty Ltd Marcadores epigenéticos de cánceres colorrectales y métodos de diagnóstico que los usan
US8481292B2 (en) 2010-09-21 2013-07-09 Population Genetics Technologies Litd. Increasing confidence of allele calls with molecular counting
WO2012038049A2 (en) 2010-09-22 2012-03-29 Roche Diagnostics Gmbh Amplification of distant nucleic acid targets using engineered primers
CN103124796B (zh) 2010-10-04 2016-08-03 霍夫曼-拉罗奇有限公司 用于pcr反应缓冲液中的细胞裂解的方法
EP2625284B1 (en) 2010-10-04 2015-01-28 Roche Diagniostics GmbH Method for cell lysis in a rt-pcr reaction buffer
CA2810316A1 (en) 2010-10-04 2012-04-12 F. Hoffmann-La Roche Ag Method for cell lysis and pcr within the same reaction vessel
DK2625295T3 (da) 2010-10-08 2019-06-11 Harvard College High-throughput-immunsekvensering
ES2966040T3 (es) 2010-10-08 2024-04-18 Harvard College Alto rendimiento de células individuales con código de barra
GB201017011D0 (en) 2010-10-08 2010-11-24 Skova Services Ltd Assay
US8725422B2 (en) 2010-10-13 2014-05-13 Complete Genomics, Inc. Methods for estimating genome-wide copy number variations
GB201017978D0 (en) 2010-10-25 2010-12-08 Oxitec Ltd Multiplex amplification and detection
KR101420094B1 (ko) 2010-10-27 2014-07-17 (주)바이오니아 다양한 생체시료분석을 위한 전자동실시간정량증폭장비, 다양한 생체시료분석을 위한 자동정제 및 반응준비 장치, 전자동 핵산정제 및 실시간 정량 유전자증폭 방법, 전자동 핵산정제방법, 실시간정량ｐｃｒ을 이용한 병원균의 전자동 생균수검사방법, 정량면역ｐｃｒ을 이용한 전자동 항원농도획득방법 및 타겟항원에 라벨링된 부착용 타겟핵산의 정제방법
US20130224116A1 (en) 2010-11-05 2013-08-29 TransBio Ltd. Markers of Endothelial Progenitor Cells and Uses Thereof
RU2451086C1 (ru) * 2010-12-03 2012-05-20 Государственное образовательное учреждение высшего профессионального образования Смоленская государственная медицинская академия федерального агентства по здравоохранению и социальному развитию Способ детекции специфических нуклеотидных последовательностей и нуклеотидных замен с помощью пцр в режиме реального времени с эффектом гашения флуоресценции зонда праймером
US8569254B2 (en) 2010-12-10 2013-10-29 National Yang Ming University Methods for modulating the expression and aggregation of CAG-expanded gene product in cells and methods for identifying agents useful for doing the same
EP2465945A1 (en) 2010-12-17 2012-06-20 F. Hoffmann-La Roche AG Generic matrix for control nucleic acids
WO2012087135A1 (en) 2010-12-22 2012-06-28 Academisch Ziekenhuis Leiden H.O.D.N. Lumc Genetic markers specific for clostridium difficile ribotypes 027 (nap01/b1; rt 027) and 078 (nap7/8; rt 078) and their use
EP2659408B1 (en) 2010-12-29 2019-03-27 Life Technologies Corporation Time-warped background signal for sequencing-by-synthesis operations
US20130060482A1 (en) 2010-12-30 2013-03-07 Life Technologies Corporation Methods, systems, and computer readable media for making base calls in nucleic acid sequencing
US10241075B2 (en) 2010-12-30 2019-03-26 Life Technologies Corporation Methods, systems, and computer readable media for nucleic acid sequencing
WO2013025998A1 (en) 2011-08-18 2013-02-21 Life Technologies Corporation Methods, systems, and computer readable media for making base calls in nucleic acid sequencing
WO2012092455A2 (en) 2010-12-30 2012-07-05 Life Technologies Corporation Models for analyzing data from sequencing-by-synthesis operations
EP2663652B1 (en) 2011-01-11 2018-11-14 Roche Diagniostics GmbH High resolution melting analysis as a prescreening tool
MX340258B (es) 2011-01-11 2016-07-01 Seegene Inc Detección de secuencias de ácido nucleico objetivo mediante ensayo de escisión y extensión del pto.
US9096892B1 (en) 2011-02-04 2015-08-04 K2 Biomicrosystems, LLC Nucleic acid amplification and monitoring apparatus
WO2012108864A1 (en) 2011-02-08 2012-08-16 Illumina, Inc. Selective enrichment of nucleic acids
FR2971256B1 (fr) 2011-02-09 2024-09-27 Bio Rad Pasteur Combinaison de biomarqueurs pour la detection et l'evaluation d'une fibrose hepatique
FR2971258B1 (fr) 2011-02-09 2020-12-04 Bio Rad Pasteur Combinaison de biomarqueurs pour le pronostic d'une reponse ou non-reponse a un traitement anti-vhc
FR2971257B1 (fr) 2011-02-09 2020-12-04 Bio Rad Pasteur Combinaison de biomarqueurs pour le pronostic d'une reponse ou non-reponse a un traitement anti-vhc
SI3270141T1 (sl) 2011-03-08 2021-03-31 Universite Laval Fluidična centripetalna naprava
EP3878975A1 (en) 2011-04-08 2021-09-15 Life Technologies Corporation Phase-protecting reagent flow orderings for use in sequencing-by-synthesis
EP3709025A1 (en) 2011-04-15 2020-09-16 Becton, Dickinson and Company Scanning real-time microfluidic thermocycler and methods for synchronized thermocycling and scanning optical detection
US20140106354A1 (en) 2011-04-18 2014-04-17 Garvan Institute Of Medical Research Method of Diagnosing Cancer
CN103635593B (zh) * 2011-05-04 2018-03-20 生物概念股份有限公司 用于检测核酸序列变体的方法
EP2707507B1 (en) 2011-05-09 2017-11-01 Fluidigm Corporation Probe based nucleic acid detection
WO2012153153A1 (en) 2011-05-11 2012-11-15 Diagon Kft. Procedure for rapid determination of viruses using nucleic acid-based molecular diagnostics, and a kit for this purpose
US8663919B2 (en) 2011-05-18 2014-03-04 Life Technologies Corporation Chromosome conformation analysis
WO2013048583A2 (en) 2011-05-24 2013-04-04 Elitech Holding B.V. Detection of methicillin-resistant staphylococcus aureus
US9034581B2 (en) 2011-05-26 2015-05-19 Roche Molecular Systems, Inc. Compositions and methods for detection of Staphylococcus aureus
WO2012166913A1 (en) * 2011-06-01 2012-12-06 Streck, Inc. Rapid thermocycler system for rapid amplification of nucleic acids and related methods
US20140186845A1 (en) 2011-06-14 2014-07-03 Universite Paul Sabatier - Toulouse Iii Methods for determining the expression level of a gene of interest including correction of rt-qpcr data for genomic dna-derived signals
SG2014010276A (en) 2011-08-18 2014-03-28 Nestec Sa Compositions and methods for detecting allelic variants
US10704164B2 (en) 2011-08-31 2020-07-07 Life Technologies Corporation Methods, systems, computer readable media, and kits for sample identification
US20150087537A1 (en) 2011-08-31 2015-03-26 Life Technologies Corporation Methods, Systems, Computer Readable Media, and Kits for Sample Identification
US10385475B2 (en) 2011-09-12 2019-08-20 Adaptive Biotechnologies Corp. Random array sequencing of low-complexity libraries
US9644241B2 (en) 2011-09-13 2017-05-09 Interpace Diagnostics, Llc Methods and compositions involving miR-135B for distinguishing pancreatic cancer from benign pancreatic disease
EP2756101B1 (en) 2011-09-15 2018-05-23 David A. Shafer Probe: antiprobe compositions for high specificity dna or rna detection
KR102087062B1 (ko) 2011-09-16 2020-03-11 렉소겐 게엠베하 핵산 전사 방법
EP2570487A1 (en) 2011-09-16 2013-03-20 Lexogen GmbH Nucleic acid transcription method
EP2758546B1 (en) 2011-09-23 2017-11-22 Roche Diagnostics GmbH Use of g-clamp for improved allele-specific pcr
CN103945931B (zh) 2011-09-26 2017-03-22 基因技术股份公司 高效的小体积核酸合成
WO2013049613A1 (en) 2011-09-29 2013-04-04 Luminex Corporation Hydrolysis probes
US9416153B2 (en) 2011-10-11 2016-08-16 Enzo Life Sciences, Inc. Fluorescent dyes
JP2014531908A (ja) 2011-10-14 2014-12-04 プレジデント アンド フェローズ オブ ハーバード カレッジ 構造アッセンブリによる配列決定
JP6420148B2 (ja) 2011-10-14 2018-11-07 ベクトン・ディキンソン・アンド・カンパニーＢｅｃｔｏｎ， Ｄｉｃｋｉｎｓｏｎ Ａｎｄ Ｃｏｍｐａｎｙ 矩形波サーマルサイクリング
AU2012325791B2 (en) 2011-10-21 2018-04-05 Adaptive Biotechnologies Corporation Quantification of adaptive immune cell genomes in a complex mixture of cells
US10837879B2 (en) 2011-11-02 2020-11-17 Complete Genomics, Inc. Treatment for stabilizing nucleic acid arrays
CN104040238B (zh) 2011-11-04 2017-06-27 汉迪拉布公司 多核苷酸样品制备装置
US9447458B2 (en) 2011-11-16 2016-09-20 Canon U.S. Life Sciences, Inc. Detection of neighboring variants
US9260714B2 (en) 2011-12-02 2016-02-16 Roche Molecular Systems, Inc. Suppression of non-specific amplification with high-homology oligonucleotides
EP3904536B1 (en) 2011-12-09 2025-10-29 Adaptive Biotechnologies Corporation Diagnosis of lymphoid malignancies and minimal residual disease detection
US9499865B2 (en) 2011-12-13 2016-11-22 Adaptive Biotechnologies Corp. Detection and measurement of tissue-infiltrating lymphocytes
WO2013093432A1 (en) 2011-12-19 2013-06-27 David Kelly Method for enriching and detection of variant target nucleic acids
US8981318B1 (en) 2011-12-30 2015-03-17 Gene Capture, Inc. Multi-dimensional scanner for nano-second time scale signal detection
US9194840B2 (en) 2012-01-19 2015-11-24 Life Technologies Corporation Sensor arrays and methods for making same
WO2013112458A1 (en) 2012-01-24 2013-08-01 Beth Israel Deaconess Medical Center, Inc. Novel chrebp isoforms and methods using the same
US9864846B2 (en) 2012-01-31 2018-01-09 Life Technologies Corporation Methods and computer program products for compression of sequencing data
US9515676B2 (en) 2012-01-31 2016-12-06 Life Technologies Corporation Methods and computer program products for compression of sequencing data
WO2013113748A1 (en) * 2012-02-02 2013-08-08 Primer Design Ltd Method for detecting and genotyping target nucleic acid
CN107326085B (zh) 2012-02-03 2022-05-03 加州理工学院 多路生化测定中信号的编码和解码
WO2013123125A1 (en) 2012-02-17 2013-08-22 President And Fellows Of Harvard College Assembly of nucleic acid sequences in emulsions
WO2013124743A1 (en) 2012-02-22 2013-08-29 Population Genetics Technologies Ltd. Compositions and methods for intramolecular nucleic acid rearrangement ii
ES2741099T3 (es) 2012-02-28 2020-02-10 Agilent Technologies Inc Método de fijación de una secuencia de recuento para una muestra de ácido nucleico
JP5976849B2 (ja) 2012-03-05 2016-08-24 シージーン アイエヌシー ＰＴＯ切断及び伸長アッセイによるターゲット核酸配列でのヌクレオチド変異検出｛ＤｅｔｅｃｔｉｏｎｏｆＮｕｃｌｅｏｔｉｄｅＶａｒｉａｔｉｏｎｏｎＴａｒｇｅｔＮｕｃｌｅｉｃＡｃｉｄＳｅｑｕｅｎｃｅｂｙＰＴＯＣｌｅａｖａｇｅａｎｄＥｘｔｅｎｓｉｏｎＡｓｓａｙ｝
EP2823060B1 (en) 2012-03-05 2018-02-14 Adaptive Biotechnologies Corporation Determining paired immune receptor chains from frequency matched subunits
EP2828400B1 (en) 2012-03-21 2018-09-19 Roche Diagniostics GmbH Methods for assessing rna quality
US9803239B2 (en) 2012-03-29 2017-10-31 Complete Genomics, Inc. Flow cells for high density array chips
BR112014027309A2 (pt) 2012-05-08 2017-07-11 Adaptive Biotechnologies Corp composições e métodos para medir e calibrar a tendên-cia de amplificação em reações de pcr multiplexadas
DK3594366T3 (da) 2012-05-11 2021-09-13 Clinical Genomics Pty Ltd Diagnostisk genmarkørpanel
US9646132B2 (en) 2012-05-11 2017-05-09 Life Technologies Corporation Models for analyzing data from sequencing-by-synthesis operations
EP3608421B1 (en) 2012-05-18 2022-06-29 Clinical Genomics Pty. Ltd. A method of screening for colorectal cancer
EP2855704A4 (en) * 2012-05-25 2016-03-16 Accugenomics Inc NUCLEIC ACID AMPLIFICATION AND USE THEREOF
US9914967B2 (en) 2012-06-05 2018-03-13 President And Fellows Of Harvard College Spatial sequencing of nucleic acids using DNA origami probes
US9628676B2 (en) 2012-06-07 2017-04-18 Complete Genomics, Inc. Imaging systems with movable scan mirrors
US9488823B2 (en) 2012-06-07 2016-11-08 Complete Genomics, Inc. Techniques for scanned illumination
WO2013188839A1 (en) 2012-06-14 2013-12-19 Life Technologies Corporation Novel compositions, methods and kits for real time polymerase chain reaction (pcr)
US9384320B2 (en) 2012-07-19 2016-07-05 President And Fellows Of Harvard College Methods of storing information using nucleic acids
US9932628B2 (en) 2012-07-27 2018-04-03 Gen-Probe Incorporated Dual reference calibration method and system for quantifying polynucleotides
AU2013202808B2 (en) 2012-07-31 2014-11-13 Gen-Probe Incorporated System and method for performing multiplex thermal melt analysis
EP2880184B1 (en) 2012-08-03 2021-03-31 California Institute of Technology Multiplexing and quantification in pcr with reduced hardware and requirements
US9957557B2 (en) 2012-08-13 2018-05-01 Life Genetics Lab, Llc Development of a highly sensitive quantification system for assessing DNA degradation and quality in forensic samples
US10993418B2 (en) 2012-08-13 2021-05-04 Life Genetics Lab, Llc Method for measuring tumor burden in patient derived xenograft (PDX) mice
US9982313B2 (en) 2012-08-17 2018-05-29 Roche Molecular Systems, Inc. Compositions and methods for detection of herpes simplex virus 1 and 2
EP3967773A1 (en) 2012-09-10 2022-03-16 BioFire Diagnostics, LLC Multiple amplification cycle detection
US9914968B2 (en) 2012-09-26 2018-03-13 Cepheid Honeycomb tube
CN105189779B (zh) 2012-10-01 2018-05-11 适应生物技术公司 通过适应性免疫受体多样性和克隆性表征进行的免疫能力评估
US10176293B2 (en) 2012-10-02 2019-01-08 Roche Molecular Systems, Inc. Universal method to determine real-time PCR cycle threshold values
CA2887302C (en) 2012-10-09 2021-11-09 University Of Utah Research Foundation Monitoring temperature with fluorescence
US10329608B2 (en) 2012-10-10 2019-06-25 Life Technologies Corporation Methods, systems, and computer readable media for repeat sequencing
EP2722399A1 (en) 2012-10-18 2014-04-23 Roche Diagniostics GmbH Method for preventing high molecular weight products during amplification
US9476089B2 (en) 2012-10-18 2016-10-25 President And Fellows Of Harvard College Methods of making oligonucleotide probes
CN103114131B (zh) 2012-11-30 2018-10-02 珠海市坤元农业科技有限公司 一种引物中部序列干扰pcr技术
GB2519255B (en) 2013-02-01 2016-01-06 Univ California Methods for genome assembly and haplotype phasing
US9411930B2 (en) 2013-02-01 2016-08-09 The Regents Of The University Of California Methods for genome assembly and haplotype phasing
EP2770065B1 (en) 2013-02-25 2017-12-13 Seegene, Inc. Detection of nucleotide variation on target nucleic acid sequence
WO2014142575A1 (en) 2013-03-13 2014-09-18 Seegene, Inc. Quantification of target nucleic acid using melting peak analysis
US20140296080A1 (en) 2013-03-14 2014-10-02 Life Technologies Corporation Methods, Systems, and Computer Readable Media for Evaluating Variant Likelihood
AU2013202805B2 (en) 2013-03-14 2015-07-16 Gen-Probe Incorporated System and method for extending the capabilities of a diagnostic analyzer
AU2013202788B2 (en) * 2013-03-14 2015-10-01 Gen-Probe Incorporated Indexing signal detection module
US10563240B2 (en) 2013-03-14 2020-02-18 Life Technologies Corporation High efficiency, small volume nucleic acid synthesis
US20140273181A1 (en) 2013-03-15 2014-09-18 Biofire Diagnostics, Inc. Compact optical system for substantially simultaneous monitoring of samples in a sample array
WO2014144640A1 (en) 2013-03-15 2014-09-18 Abbott Laboratories Automated diagnostic analyzers having vertically arranged carousels and related methods
EP2972402B1 (en) 2013-03-15 2023-12-20 Abbott Laboratories Diagnostic analyzers with pretreatment carousels and related methods
EP2972404B1 (en) 2013-03-15 2021-11-24 Abbott Laboratories Automated diagnostic analyzers having rear accessible track systems and related methods
EP3486328B1 (en) 2013-03-15 2020-11-11 Avellino Lab USA, Inc. Methods for improved isolation of genomic dna templates for allele detection
WO2014144495A1 (en) 2013-03-15 2014-09-18 Abvitro, Inc. Single cell bar-coding for antibody discovery
US10933417B2 (en) 2013-03-15 2021-03-02 Nanobiosym, Inc. Systems and methods for mobile device analysis of nucleic acids and proteins
JP6441893B2 (ja) 2013-03-19 2018-12-19 ディレクティド・ジェノミクス・エル・エル・シー 標的配列の濃縮
CN105492624B (zh) 2013-04-23 2019-10-18 恩姆菲舍尔科技公司 熔解曲线分析
CA2912312C (en) 2013-05-20 2023-01-24 Board Of Trustees Of The University Of Arkansas Gep5 model for multiple myeloma
EP3957743A1 (en) 2013-06-19 2022-02-23 Luminex Corporation Real-time multiplexed hydrolysis probe assay
CN111349692B (zh) * 2013-06-25 2023-10-13 C.威特沃 进行聚合酶链式反应的方法及其相关应用
EP3014251A1 (en) 2013-06-28 2016-05-04 Streck Inc. Devices for real-time polymerase chain reaction
US9708657B2 (en) 2013-07-01 2017-07-18 Adaptive Biotechnologies Corp. Method for generating clonotype profiles using sequence tags
JP6050555B2 (ja) 2013-07-15 2016-12-21 シージーン アイエヌシー Ｐｔｏの切断及び延長−依存的固定化オリゴヌクレオチドハイブリダイゼーションを用いたターゲット核酸配列の検出
US9926597B2 (en) 2013-07-26 2018-03-27 Life Technologies Corporation Control nucleic acid sequences for use in sequencing-by-synthesis and methods for designing the same
EP4321867B1 (en) 2013-08-09 2025-10-01 Luminex Corporation Probes for improved melt discrimination and multiplexing in nucleic acid assays
WO2015022669A2 (en) 2013-08-15 2015-02-19 Centre Hospitalier Universitarie Vaudois Methods for typing hla alleles
CN105683980B (zh) 2013-10-04 2018-08-24 生命科技股份有限公司 在使用终止化学物质的测序中建立分阶段效应模型的方法和系统
ES2925313T3 (es) 2013-10-09 2022-10-14 Fluoresentric Inc Método para la detección de secuencias de ácido nucleico diana
US10072288B2 (en) 2013-11-11 2018-09-11 Roche Molecular Systems, Inc. Detecting single nucleotide polymorphism using overlapped primer and melting probe
JP6672147B2 (ja) 2013-11-15 2020-03-25 アベリノ ラボ ユーエスエー インコーポレイテッドＡｖｅｌｌｉｎｏ Ｌａｂ ＵＳＡ， Ｉｎｃ． 眼疾患に関連したアレルのマルチプレックス検出方法
DK3077539T3 (en) 2013-12-02 2018-11-19 Personal Genome Diagnostics Inc Procedure for evaluating minority variations in a sample
US9476853B2 (en) 2013-12-10 2016-10-25 Life Technologies Corporation System and method for forming microwells
EP3080605B1 (en) 2013-12-11 2019-02-20 The Regents of the University of California Method for labeling dna fragments to reconstruct physical linkage and phase
CN106232827A (zh) 2014-02-21 2016-12-14 哈佛学院董事及会员团体 变构蛋白的从头设计
ES2741740T3 (es) 2014-03-05 2020-02-12 Adaptive Biotechnologies Corp Métodos que usan moléculas sintéticas que contienen segmentos de nucleótidos aleatorios
WO2015138343A1 (en) 2014-03-10 2015-09-17 Click Diagnostics, Inc. Cartridge-based thermocycler
EP2921556A1 (en) 2014-03-21 2015-09-23 Lexogen GmbH Copy number preserving RNA analysis method
WO2015147370A1 (en) 2014-03-28 2015-10-01 Seegene, Inc. Detection of target nucleic acid sequences using different detection temperatures
US11390921B2 (en) 2014-04-01 2022-07-19 Adaptive Biotechnologies Corporation Determining WT-1 specific T cells and WT-1 specific T cell receptors (TCRs)
US10066265B2 (en) 2014-04-01 2018-09-04 Adaptive Biotechnologies Corp. Determining antigen-specific t-cells
EP3126524B1 (en) 2014-04-04 2020-07-22 Affymetrix, Inc. Improved compositions and methods for molecular inversion probe assays
BE1022048B1 (nl) * 2014-04-11 2016-02-10 Ugentec Bvba Methoden voor fluorescentie data correctie
ES2777529T3 (es) 2014-04-17 2020-08-05 Adaptive Biotechnologies Corp Cuantificación de genomas de células inmunitarias adaptativas en una mezcla compleja de células
WO2015191723A1 (en) 2014-06-10 2015-12-17 University Of Southern California Usc Discrete elements for 3d microfluidics
DE102014212657B4 (de) * 2014-06-30 2016-03-10 Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. System und Verfahren zur bedarfsgerechten Zuführung von Beleuchtungsenergie an Pflanzen
GB201411567D0 (en) * 2014-06-30 2014-08-13 Epistem Ltd Quantification methods
US10526641B2 (en) 2014-08-01 2020-01-07 Dovetail Genomics, Llc Tagging nucleic acids for sequence assembly
DE102014215352A1 (de) * 2014-08-04 2016-02-04 Berthold Technologies Gmbh & Co. Kg Messgerät und Verfahren zum Bestimmen einer Messgröße
KR20220155394A (ko) 2014-08-11 2022-11-22 루미넥스 코포레이션 핵산 검정에서 개선된 용융 판별 및 멀티플렉싱을 위한 프로브
SG10201911069WA (en) 2014-09-15 2020-01-30 Abvitro Llc High-throughput nucleotide library sequencing
AU2015318823A1 (en) 2014-09-15 2016-03-24 Garvan Institute Of Medical Research Methods for diagnosis, prognosis and monitoring of breast cancer and reagents therefor
CN113699113A (zh) * 2014-09-16 2021-11-26 桑格摩治疗股份有限公司 用于造血干细胞中核酸酶介导的基因组工程化和校正的方法和组合物
SG11201702416YA (en) 2014-09-30 2017-04-27 Genetic Technologies Ltd Methods for assessing risk of developing breast cancer
US10676787B2 (en) 2014-10-13 2020-06-09 Life Technologies Corporation Methods, systems, and computer-readable media for accelerated base calling
US10392663B2 (en) 2014-10-29 2019-08-27 Adaptive Biotechnologies Corp. Highly-multiplexed simultaneous detection of nucleic acids encoding paired adaptive immune receptor heterodimers from a large number of samples
WO2016077763A1 (en) 2014-11-13 2016-05-19 The Board Of Trustees Of The University Of Illinois Bio-engineered hyper-functional "super" helicases
US10246701B2 (en) 2014-11-14 2019-04-02 Adaptive Biotechnologies Corp. Multiplexed digital quantitation of rearranged lymphoid receptors in a complex mixture
US9745618B2 (en) 2014-11-19 2017-08-29 Roche Molecular Systems, Inc. Photoblocked probes and methods for sequential detection of nucleic acids
AU2015353581A1 (en) 2014-11-25 2017-06-15 Adaptive Biotechnologies Corporation Characterization of adaptive immune response to vaccination or infection using immune repertoire sequencing
US9920381B2 (en) 2014-12-02 2018-03-20 Roche Molecular Systems, Inc. Compositions and methods for detecting MECC-containing methicillin-resistant Staphylococcus aureus
EP3229959B1 (en) 2014-12-09 2019-05-15 Life Technologies Corporation High efficiency, small volume nucleic acid synthesis
JP2017537657A (ja) 2014-12-11 2017-12-21 ニユー・イングランド・バイオレイブス・インコーポレイテツド 標的配列の濃縮
US9909169B2 (en) 2014-12-17 2018-03-06 Roche Molecular Systems, Inc. Allele-specific amplification of nucleic acids using blocking oligonucleotides for wild type suppression
EP4029606A1 (en) 2014-12-31 2022-07-20 Visby Medical, Inc. Molecular diagnostic testing
US10421993B2 (en) 2015-02-11 2019-09-24 Paragon Genomics, Inc. Methods and compositions for reducing non-specific amplification products
KR102721363B1 (ko) 2015-02-17 2024-10-23 더브테일 제노믹스 엘엘씨 핵산 서열 어셈블리
ES2858306T3 (es) 2015-02-24 2021-09-30 Adaptive Biotechnologies Corp Método para determinar el estado de HLA mediante secuenciación del repertorio inmunitario
US9708647B2 (en) 2015-03-23 2017-07-18 Insilixa, Inc. Multiplexed analysis of nucleic acid hybridization thermodynamics using integrated arrays
WO2016154540A1 (en) 2015-03-26 2016-09-29 Dovetail Genomics Llc Physical linkage preservation in dna storage
US9957393B2 (en) 2015-03-30 2018-05-01 Enzo Biochem, Inc. Monoazo dyes with cyclic amine as fluorescence quenchers
AU2016242967B2 (en) 2015-04-01 2021-07-01 Adaptive Biotechnologies Corp. Method of identifying human compatible T cell receptors specific for an antigenic target
ES2961374T3 (es) 2015-04-24 2024-03-11 Atila Biosystems Incorporated Amplificación con cebadores de composición de nucleótidos limitada
WO2016179090A1 (en) * 2015-05-01 2016-11-10 Vanderbilt University Monitoring and analysis of nucleic acid hybridization state and amplification using l-dna
EP4220645A3 (en) 2015-05-14 2023-11-08 Life Technologies Corporation Barcode sequences, and related systems and methods
KR102077577B1 (ko) 2015-06-04 2020-02-17 가부시키가이샤 미즈호 메디 복수의 표적 핵산의 검출 키트 및 그것을 사용하는 검출 방법
JP2018525305A (ja) 2015-06-08 2018-09-06 ザ・ユニバーシティ・オブ・シカゴＴｈｅ Ｕｎｉｖｅｒｓｉｔｙ Ｏｆ Ｃｈｉｃａｇｏ 単分散貴金属ナノ結晶の双角錐鋳型合成
US9499861B1 (en) 2015-09-10 2016-11-22 Insilixa, Inc. Methods and systems for multiplex quantitative nucleic acid amplification
JP6869233B2 (ja) 2015-09-24 2021-05-12 アブビトロ， エルエルシー 親和性−オリゴヌクレオチドコンジュゲートおよびその使用
US10928392B2 (en) 2015-09-25 2021-02-23 Abvitro Llc High throughput process for T cell receptor target identification of natively-paired T cell receptor sequences
US11266730B2 (en) 2015-09-29 2022-03-08 The General Hospital Corporation Methods of treating and diagnosing disease using biomarkers for BCG therapy
AU2016341198B2 (en) 2015-10-19 2023-03-09 Dovetail Genomics, Llc Methods for genome assembly, haplotype phasing, and target independent nucleic acid detection
US10808287B2 (en) 2015-10-23 2020-10-20 Rapid Pathogen Screening, Inc. Methods and devices for accurate diagnosis of infections
USD799715S1 (en) 2015-10-23 2017-10-10 Gene POC, Inc. Fluidic centripetal device
WO2017083852A1 (en) 2015-11-13 2017-05-18 MOORE, Tara Methods for the treatment of corneal dystrophies
CN108350407B (zh) 2015-12-11 2022-07-08 杰诺玛迪克斯公司 用于核酸扩增的管密封系统和方法
EP3391029B1 (en) 2015-12-18 2023-11-08 BioFire Defense, LLC Solid fluorescence standard
ES2976360T3 (es) 2016-01-28 2024-07-30 Univ Melbourne Métodos para evaluar el riesgo de desarrollar cáncer colorrectal
US10975417B2 (en) 2016-02-23 2021-04-13 Dovetail Genomics, Llc Generation of phased read-sets for genome assembly and haplotype phasing
WO2017155858A1 (en) 2016-03-07 2017-09-14 Insilixa, Inc. Nucleic acid sequence identification using solid-phase cyclic single base extension
CA3009080C (en) 2016-03-10 2023-10-10 Pioneer Hi-Bred International, Inc. Light-mediated polymerase chain reaction amplification and product detection system and methods of use
CN108779500B (zh) 2016-03-11 2022-12-13 豪夫迈·罗氏有限公司 用于检测寨卡病毒的组合物和方法
WO2017185067A1 (en) 2016-04-22 2017-10-26 Click Diagnostics, Inc. Printed circuit board heater for an amplification module
US10619205B2 (en) 2016-05-06 2020-04-14 Life Technologies Corporation Combinatorial barcode sequences, and related systems and methods
WO2017197040A1 (en) 2016-05-11 2017-11-16 Click Diagnostics, Inc. Devices and methods for nucleic acid extraction
CN109477101B (zh) 2016-05-13 2022-11-18 多弗泰尔基因组学有限责任公司 从保存的样品中回收长范围连锁信息
EP3458597B1 (en) 2016-05-18 2022-09-07 Roche Diagnostics GmbH Quantitative real time pcr amplification using an electrowetting-based device
CN109154023B (zh) 2016-05-27 2022-09-20 豪夫迈·罗氏有限公司 用于检测阴道毛滴虫的组合物和方法
US10612101B2 (en) 2016-05-27 2020-04-07 Roche Molecular Systems, Inc. Compositions and methods for detection of Mycoplasma genitalium
US20170351807A1 (en) 2016-06-01 2017-12-07 Life Technologies Corporation Methods and systems for designing gene panels
US20170356025A1 (en) 2016-06-14 2017-12-14 Roche Molecular Systems, Inc. Internal control probes for improving pcr assay performance
CN109642252A (zh) 2016-06-17 2019-04-16 加州理工学院 核酸反应及相关方法和组合物
RU2691763C2 (ru) * 2016-06-21 2019-06-18 Федеральное государственное бюджетное учреждение науки Институт аналитического приборостроения Российской академии наук (ИАП РАН) Устройство для одновременного контроля в реальном масштабе времени множества амплификаций нуклеиновой кислоты
CN110325652A (zh) 2016-06-29 2019-10-11 易捷仪器诊断股份有限公司 使用流动池检测分子的装置和方法
US10626454B2 (en) 2016-07-27 2020-04-21 SeqMatic, LLC Compositions and methods for nucleic acid amplification and analysis
ES2926513T3 (es) 2016-07-29 2022-10-26 Juno Therapeutics Inc Métodos para evaluar la presencia o ausencia de virus competente en replicación
WO2018024562A1 (en) 2016-08-02 2018-02-08 Roche Diagnostics Gmbh Helper oligonucleotide for improving efficiency of amplification and detection/quantitation of nucleic acids
JP7221199B2 (ja) 2016-09-15 2023-02-20 エフ． ホフマン－ラ ロシュ エージー． マルチプレックスｐｃｒの実施方法
US10428325B1 (en) 2016-09-21 2019-10-01 Adaptive Biotechnologies Corporation Identification of antigen-specific B cell receptors
US10487358B2 (en) 2016-09-23 2019-11-26 Grail, Inc. Methods of preparing and analyzing cell-free nucleic acid sequencing libraries
BR112019005748A2 (pt) 2016-09-24 2019-06-18 Abvitro Llc afinidade-conjugados de oligonucleotídeo e usos destes
US9851345B1 (en) 2016-10-12 2017-12-26 Viasphere, Llc Compositions and methods for disease diagnosis using single cell analysis
JP6786618B2 (ja) 2016-10-17 2020-11-18 オリンパス株式会社 標的核酸分子の検出方法
CA3040930A1 (en) 2016-11-07 2018-05-11 Grail, Inc. Methods of identifying somatic mutational signatures for early cancer detection
US10793923B2 (en) 2016-11-09 2020-10-06 Roche Molecular Systems, Inc. Compositions and methods for detection of BK virus
CN110291205A (zh) 2016-12-12 2019-09-27 塞弗德公司 自动反应盒中的整合的免疫pcr和核酸分析
EP3551768B1 (en) 2016-12-12 2024-03-06 Grail, LLC Methods for tagging and amplifying rna template molecules for preparing sequencing libraries
US20190211377A1 (en) 2016-12-22 2019-07-11 Roche Molecular Systems, Inc. Cobra probes to detect a marker for epidemic ribotypes of clostridium difficile
WO2018119399A1 (en) 2016-12-23 2018-06-28 Grail, Inc. Methods for high efficiency library preparation using double-stranded adapters
US10793901B2 (en) 2016-12-28 2020-10-06 Roche Molecular Systems, Inc. Reversibly protected nucleotide reagents with high thermal stability
KR102349259B1 (ko) 2017-01-04 2022-01-07 엠쥐아이 테크 컴퍼니 엘티디. 친화성 시약을 이용한 핵산 시퀀싱
CN110382716A (zh) 2017-01-10 2019-10-25 佰隆基因公司 用于减少引物延伸反应中产生的冗余分子条形码的方法和组合物
WO2018175715A1 (en) 2017-03-24 2018-09-27 Board Of Supervisors Of Louisiana State University And Agricultural And Mechanical College Herpes simplex virus type-1(hsv-1) vaccine strain vc2 generating an anti-ehv-1 immune response
JP6994513B2 (ja) 2017-03-24 2022-03-04 ジェン－プローブ・インコーポレーテッド 試料中のウイルス病原体を検出するための組成物および方法
US11274344B2 (en) 2017-03-30 2022-03-15 Grail, Inc. Enhanced ligation in sequencing library preparation
WO2018183897A1 (en) 2017-03-31 2018-10-04 Grail, Inc. Higher target capture efficiency using probe extension
US11584958B2 (en) 2017-03-31 2023-02-21 Grail, Llc Library preparation and use thereof for sequencing based error correction and/or variant identification
CN110997940A (zh) 2017-04-28 2020-04-10 阿维利诺美国实验室股份有限公司 用于检测与圆锥角膜相关的等位基因的方法
WO2018213803A1 (en) 2017-05-19 2018-11-22 Neon Therapeutics, Inc. Immunogenic neoantigen identification
US20190093155A1 (en) 2017-05-25 2019-03-28 Roche Molecular Systems, Inc. Multiplex Nucleic Acid Amplification Assay
MA49352A (fr) 2017-05-26 2020-04-08 Abvitro Llc Séquençage de bibliothèque de polynucléotides à haut rendement et analyse de transcriptome
US20180363044A1 (en) 2017-06-14 2018-12-20 Roche Molecular Systems, Inc. Compositions and methods for improving the thermal stability of nucleic acid amplification reagents
US11450121B2 (en) 2017-06-27 2022-09-20 The Regents Of The University Of California Label-free digital brightfield analysis of nucleic acid amplification
WO2019006427A1 (en) 2017-06-29 2019-01-03 Juno Therapeutics, Inc. WALL MODEL FOR ASSESSING TOXICITIES ASSOCIATED WITH IMMUNOTHERAPIES
CN111032209A (zh) 2017-07-10 2020-04-17 简·探针公司 分析系统和方法
US10760137B2 (en) 2017-07-18 2020-09-01 Roche Molecular Systems, Inc. Compositions and methods for detection of Babesia
KR20200064060A (ko) 2017-07-29 2020-06-05 주노 쎄러퓨티크스 인코퍼레이티드 재조합 수용체를 발현하는 세포 증폭용 시약
EP3676400A4 (en) 2017-08-31 2021-06-02 Seegene, Inc. EVALUATING THE PERFORMANCE OF COMPONENTS USING A PAIR OF DIMERING PRIMERS
MA50057A (fr) 2017-09-01 2020-07-08 Juno Therapeutics Inc Expression génique et évaluation d'un risque de développement d'une toxicité suite à une thérapie cellulaire
US20200263170A1 (en) 2017-09-14 2020-08-20 Grail, Inc. Methods for preparing a sequencing library from single-stranded dna
EP4269583B1 (en) 2017-09-28 2025-12-31 Grail, Inc. ENRICHEMENT OF SHORT NUCLEIC ACID FRAGMENTS IN SEQUENCING BANK PREPARATION
WO2019063661A1 (en) 2017-09-29 2019-04-04 Roche Diagnostics Gmbh COMPOSITIONS AND METHODS FOR THE DETECTION OF TRICHOMONAS VAGINALIS
US11401546B2 (en) 2017-09-29 2022-08-02 Seegene, Inc. Detection of target nucleic acid sequences by PTO cleavage and extension-dependent extension assay
WO2019084055A1 (en) 2017-10-23 2019-05-02 Massachusetts Institute Of Technology CLASSIFICATION OF GENETIC VARIATION FROM UNICELLULAR TRANSCRIPTOMS
US20210254000A1 (en) 2017-11-01 2021-08-19 Juno Therapeutics, Inc. Process for producing a t cell composition
KR20200079264A (ko) 2017-11-09 2020-07-02 비스비 메디컬, 인코포레이티드 표적 바이러스 검출을 위한 휴대용 분자 진단 디바이스 및 방법
US11078534B2 (en) 2017-11-27 2021-08-03 Roche Molecular Systems, Inc. Photocleavable nucleotide reagents with high stability
US11254980B1 (en) 2017-11-29 2022-02-22 Adaptive Biotechnologies Corporation Methods of profiling targeted polynucleotides while mitigating sequencing depth requirements
WO2021130199A2 (en) 2019-12-27 2021-07-01 F. Hoffmann-La Roche Ag Compositions and methods for detecting methicillin-resistant staphylococcus aureus
WO2019118925A1 (en) 2017-12-15 2019-06-20 Grail, Inc. Methods for enriching for duplex reads in sequencing and error correction
WO2019126803A1 (en) 2017-12-22 2019-06-27 Grail, Inc. Error removal using improved library preparation methods
WO2019135567A1 (en) 2018-01-05 2019-07-11 Seegene, Inc. . Method for determining the presence or absence of m. tuberculosis, m. bovis and m. bovis bcg in a sample
CN111902213B (zh) 2018-01-29 2022-12-06 简·探针公司 分析系统和方法
WO2019152747A1 (en) 2018-01-31 2019-08-08 Juno Therapeutics, Inc. Methods and reagents for assessing the presence or absence of replication competent virus
WO2019152543A1 (en) 2018-01-31 2019-08-08 Dovetail Genomics, Llc Sample prep for dna linkage recovery
US20190292596A1 (en) 2018-03-21 2019-09-26 Roche Molecular Systems, Inc. Modified nucleoside phosphates with high thermal stability
CN112424377A (zh) 2018-04-17 2021-02-26 克罗玛科德公司 用于多重分析的方法和系统
US11384376B2 (en) 2018-05-31 2022-07-12 Roche Molecular Systems, Inc. Reagents and methods for post-synthetic modification of nucleic acids
US12203129B2 (en) 2018-07-03 2025-01-21 ChromaCode, Inc. Formulations and signal encoding and decoding methods for massively multiplexed biochemical assays
US20210317515A1 (en) 2018-07-10 2021-10-14 Gen-Probe Incorporated Methods and systems for detecting and quantifying nucleic acids
GB201812192D0 (en) 2018-07-26 2018-09-12 Ttp Plc Variable temperature reactor, heater and control circuit for the same
SG11202101934SA (en) 2018-07-30 2021-03-30 Readcoor Llc Methods and systems for sample processing or analysis
JP2021533769A (ja) 2018-08-16 2021-12-09 ライフ テクノロジーズ コーポレーション 核酸の増幅のための試薬、混合物、キット、および方法
WO2020051156A1 (en) 2018-09-03 2020-03-12 Visby Medical, Inc. Devices and methods for antibiotic susceptibility testing
JP7221491B2 (ja) * 2018-09-18 2023-02-14 株式会社ミズホメディー 複数の標的核酸を検出するキットを用いる検出方法
US20220195515A1 (en) 2018-10-29 2022-06-23 Koninklijke Nederlandse Akademie Van Wetenschappen Single cell full length RNA sequencing
CA3117720A1 (en) 2018-11-06 2020-05-14 Juno Therapeutics, Inc. Process for producing genetically engineered t cells
US12344905B2 (en) 2018-12-03 2025-07-01 Roche Molecular Systems, Inc. Compositions and methods for detection of Candida auris
KR20210104779A (ko) 2018-12-13 2021-08-25 디엔에이 스크립트 세포 및 생체분자 상의 직접 올리고뉴클레오타이드 합성
US11512349B2 (en) 2018-12-18 2022-11-29 Grail, Llc Methods for detecting disease using analysis of RNA
CA3124763A1 (en) 2019-01-03 2020-07-09 Dna Script One pot synthesis of sets of oligonucleotides
CN113924041B (zh) 2019-03-14 2024-12-03 因斯利克萨公司 基于时间门控的荧光检测的方法和系统
BR112021020447A2 (pt) 2019-04-17 2022-05-17 Alpine Immune Sciences Inc Métodos e usos de proteínas de fusão de ligante icos variante (icosl)
CA3135278A1 (en) 2019-04-28 2020-11-05 Visby Medical, Inc. Molecular diagnostic devices with digital detection capability and wireless connectivity
EP3962654A1 (en) * 2019-05-01 2022-03-09 Luminex Corporation Apparatus and methods for thermal cycling of sample
WO2020221915A1 (en) 2019-05-02 2020-11-05 F. Hoffmann-La Roche Ag UTILIZATION OF dITP FOR PREFERENTIAL/SELECTIVE AMPLIFICATION OF RNA VERSUS DNA TARGETS BASED ON STRAND-SEPARATION TEMPERATURE
JP7592027B2 (ja) 2019-05-07 2024-11-29 エフ． ホフマン－ラ ロシュ アーゲー ナイセリア・ゴノレア（Ｎｅｉｓｓｅｒｉａ Ｇｏｎｏｒｒｏｈｅａｅ）を検出するための組成物および方法
CN114269371A (zh) 2019-06-12 2022-04-01 朱诺治疗学股份有限公司 细胞介导的细胞毒性疗法与促存活bcl2家族蛋白的抑制剂的组合疗法
EP4545965A3 (en) 2019-06-27 2025-06-11 Dovetail Genomics, LLC Methods and compositions for proximity ligation
ES2988562T3 (es) 2019-07-25 2024-11-20 Hoffmann La Roche Composiciones y procedimientos para la detección del virus de Epstein-Barr (VEB)
CN114555112A (zh) 2019-08-22 2022-05-27 朱诺治疗学股份有限公司 T细胞疗法和zeste增强子同源物2(ezh2)抑制剂的组合疗法及相关方法
EP4022086B1 (en) 2019-08-27 2025-08-06 F. Hoffmann-La Roche AG Compositions and methods for amplification and detection of hepatitis b virus rna, including hbv rna transcribed from cccdna
US11441167B2 (en) 2019-11-20 2022-09-13 Roche Molecular Systems, Inc. Compositions and methods for rapid identification and phenotypic antimicrobial susceptibility testing of bacteria and fungi
EP4073175A1 (en) 2019-12-09 2022-10-19 F. Hoffmann-La Roche AG Dicationic fluorescent dyes
EP4085149A4 (en) 2020-01-03 2024-03-06 Visby Medical, Inc. Devices and methods for antibiotic susceptibility testing
WO2021140173A1 (en) 2020-01-10 2021-07-15 Biouniversa S.R.L. Methods and uses for treating fibrotic solid tumors with bags inhibitors
EP4090734B1 (en) 2020-01-13 2024-11-06 Aspen Neuroscience, Inc. Method of differentiating neural cells and related compositions and methods of use
US20210332448A1 (en) 2020-03-09 2021-10-28 Janssen Pharmaceutica Nv Compositions and methods for quantifying integration of recombinant vector nucleic acid
CN116171333A (zh) 2020-03-09 2023-05-26 豪夫迈·罗氏有限公司 用于检测严重急性呼吸综合征冠状病毒2(sars-cov-2)、甲型流感和乙型流感的组合物和方法
WO2021185320A1 (en) 2020-03-18 2021-09-23 Mgi Tech Co., Ltd. Restoring phase in massively parallel sequencing
KR20230022868A (ko) 2020-05-13 2023-02-16 주노 쎄러퓨티크스 인코퍼레이티드 재조합 수용체를 발현하는 공여자-배치 세포의 제조 방법
US10941453B1 (en) 2020-05-20 2021-03-09 Paragon Genomics, Inc. High throughput detection of pathogen RNA in clinical specimens
CA3179313A1 (en) 2020-05-27 2021-12-02 Gillian Sue Dite Methods of assessing risk of developing a severe response to coronavirus infection
WO2021260186A1 (en) 2020-06-26 2021-12-30 Juno Therapeutics Gmbh Engineered t cells conditionally expressing a recombinant receptor, related polynucleotides and methods
US11519861B2 (en) * 2020-07-20 2022-12-06 Photothermal Spectroscopy Corp Fluorescence enhanced photothermal infrared spectroscopy and confocal fluorescence imaging
EP4237573A1 (en) 2020-10-29 2023-09-06 DNA Script Enzymatic synthesis of polynucleotide probes
CN112461806B (zh) * 2020-11-18 2022-02-11 厦门大学 基于智能手机的荧光光谱检测方法
EP4256089A1 (en) 2020-12-04 2023-10-11 F. Hoffmann-La Roche AG Compositions and methods for detection of malaria
JP7209980B2 (ja) 2020-12-11 2023-01-23 東洋紡株式会社 Ｄｎａポリメラーゼの５’→３’エキソヌクレアーゼ活性ドメインに特異的に結合する抗体
JP2024500169A (ja) 2020-12-22 2024-01-04 エフ． ホフマン－ラ ロシュ アーゲー 大ストークスシフト蛍光色素を使用して多重化リアルタイムｐｃｒを行うための方法
US20220205020A1 (en) 2020-12-30 2022-06-30 Roche Molecular Systems, Inc. Compositions and methods for detection of bacteria and fungi associated with bacterial and candida vaginosis
EP4274907A4 (en) 2021-01-08 2025-01-15 Cellanome, Inc. DEVICES AND METHODS FOR THE ANALYSIS OF BIOLOGICAL SAMPLES
JP2024505686A (ja) 2021-02-05 2024-02-07 エフ． ホフマン－ラ ロシュ アーゲー ヒトパラインフルエンザウイルス１－４（ｈｐｉｖ１－４）の検出のための組成物及び方法
US20220290221A1 (en) 2021-03-15 2022-09-15 Roche Molecular Systems, Inc. Compositions and methods for detecting severe acute respiratory syndrome coronavirus 2 (sars-cov-2) variants having spike protein mutations
WO2022233930A1 (en) 2021-05-06 2022-11-10 F. Hoffmann-La Roche Ag Compositions and methods for detecting hepatitis delta virus by a dual-target assay
WO2023283452A2 (en) 2021-07-09 2023-01-12 Cepheid High-level multiplexing reaction vessel, reagent spotting device and associated methods
WO2023004371A1 (en) 2021-07-21 2023-01-26 Aspen Neuroscience, Inc. Methods of differentiating neural cells and predicting engraftment thereof and related compositions
WO2023043280A1 (ko) 2021-09-17 2023-03-23 주식회사 씨젠 합성 비자연 염기를 포함하는 태그 올리고뉴클레오타이드를 이용한 타겟 핵산 서열의 검출
US20230121442A1 (en) 2021-10-06 2023-04-20 Johnson & Johnson Consumer Inc. Method of Quantifying Product Impact on Human Microbiome
WO2023057338A1 (en) 2021-10-06 2023-04-13 Bayer Aktiengesellschaft Integrated system for chemical, biochemical or molecular biological reactions in a microplate
WO2023079032A1 (en) 2021-11-05 2023-05-11 F. Hoffmann-La Roche Ag Compositions and methods for detection of malaria
WO2023089186A1 (en) 2021-11-22 2023-05-25 F. Hoffmann-La Roche Ag Compositions and methods for detecting vana and/or vanb genes associated with multidrug resistance
AU2022402153A1 (en) 2021-12-03 2024-06-13 Medicinal Genomics Corporation Psilocybe assay
EP4471162A4 (en) 2022-01-26 2025-12-17 Seegene Inc METHOD FOR THE DETECTION OF TARGET NUCLEAN ACIDS IN A SAMPLE
CN119384700A (zh) 2022-04-15 2025-01-28 艾斯本神经科学公司 细胞分化状态的分类方法和分化细胞的相关组合物
US11680293B1 (en) 2022-04-21 2023-06-20 Paragon Genomics, Inc. Methods and compositions for amplifying DNA and generating DNA sequencing results from target-enriched DNA molecules
US12091715B2 (en) 2022-04-21 2024-09-17 Paragon Genomics, Inc. Methods and compositions for reducing base errors of massive parallel sequencing using triseq sequencing
EP4532695A1 (en) 2022-05-25 2025-04-09 Celgene Corporation Methods of manufacturing t cell therapies
CA3260523A1 (en) 2022-06-29 2024-01-04 Actome Gmbh DETECTION OF BIOMOLECULES IN SINGLE CELLS
JP2025521750A (ja) 2022-06-30 2025-07-10 エフ． ホフマン－ラ ロシュ アーゲー クラミジア・トラコマチス（ｃｈｌａｍｙｄｉａ ｔｒａｃｈｏｍａｔｉｓ）の鼠径リンパ肉芽腫症（ｌｇｖ）血清型を検出するための組成物および方法
US20250368983A1 (en) 2022-06-30 2025-12-04 Kapa Biosystems, Inc. Controlling for tagmentation sequencing library insert size using archaeal histone-like proteins
CN115181787A (zh) * 2022-07-01 2022-10-14 广州医科大学附属第一医院（广州呼吸中心） 一种pcr扩增后的产物快速检测方法
CN119968468A (zh) 2022-07-12 2025-05-09 拓扑基因股份有限公司 Dna芯片亚微米精度大规模复制方法
EP4577674A1 (en) 2022-08-24 2025-07-02 F. Hoffmann-La Roche AG Compositions and methods for detecting monkeypox virus
WO2024059493A1 (en) 2022-09-13 2024-03-21 Medicinal Genomics Corporation Psilocybe assay
AU2023345834A1 (en) 2022-09-20 2025-03-06 Cost-Bry Pty Ltd (trading as BiomeBank) Compositions and methods for reducing endogenous sulphide in inflammatory bowel diseases
KR20250110818A (ko) 2022-11-16 2025-07-21 주식회사 씨젠 퀀칭-pto 절단 및 연장(quenching-pto cleavage and extension；q-ptoce) 어세이에 의한 타겟 핵산의 검출
WO2024123122A1 (ko) 2022-12-08 2024-06-13 주식회사 씨젠 체외 진단용 시약 제품의 연구개발 관리 장치 및 방법
EP4632760A1 (en) 2022-12-08 2025-10-15 Seegene, Inc. Method for updating research and development module of in vitro diagnostic reagent product and research and development management device
EP4638791A1 (en) 2022-12-25 2025-10-29 F. Hoffmann-La Roche AG Compositions and methods for detecting carbapenem resistant acinetobacter calcoaceticus- baumannii(crab)
EP4655411A2 (en) 2023-01-26 2025-12-03 DNA Script Enzymatic synthesis of polynucleotide probes
WO2024175749A1 (en) 2023-02-25 2024-08-29 F. Hoffmann-La Roche Ag Compositions and methods for candida species detection
WO2024182219A1 (en) 2023-02-27 2024-09-06 Adaptive Biotechnologies Corp. Therapeutic t cell receptors targeting kras g12d
WO2024184165A1 (en) 2023-03-03 2024-09-12 F. Hoffmann-La Roche Ag Compositions and methods for the detection of group b streptococcus ( streptococcus agalactiae) and clindamycin resistance gene determinants
EP4455303A1 (en) * 2023-04-28 2024-10-30 Mobidiag Oy Nucleic acid amplification process controls
WO2024235904A1 (en) 2023-05-16 2024-11-21 Roche Molecular Systems, Inc. Detection of target analytes using multimodal signal probes
WO2025027080A1 (en) 2023-08-03 2025-02-06 F. Hoffmann-La Roche Ag Use of 5'-tailed long primers to improve amplification performance when targeting short primer-binding sites
WO2025072571A1 (en) 2023-09-27 2025-04-03 Cellanome, Inc. Cell culture within microfluidic structures
WO2025202488A1 (en) 2024-03-28 2025-10-02 Roche Diagnostics Gmbh Enhancer of nucleic acid amplification
WO2025245399A1 (en) 2024-05-23 2025-11-27 Indapta Therapeutics, Inc. Method of treating cancer with natural killer cells
WO2025257150A1 (en) 2024-06-11 2025-12-18 Roche Diagnostics International Ag Computer-implemented method for early-stage prediction of pcr result

Family Cites Families (116)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US1006767A (en) 1910-01-19 1911-10-24 Gen Electric Electric hair-drier.
US1456005A (en) 1922-07-24 1923-05-22 Harris Jack Incubator
US2379474A (en) 1943-11-10 1945-07-03 Bramson Maurice Heating cabinet, incubator, and the like
US3219416A (en) 1962-10-30 1965-11-23 Scientific Industries Apparatus for the automatic chemical sequential treatment and analysis of small quantities of material
US3556659A (en) 1966-02-03 1971-01-19 Applied Physics Corp Laser-excited raman spectrometer
US3616264A (en) 1969-06-30 1971-10-26 Beckman Instruments Inc Temperature-controlled discrete sample analyzer
US3718133A (en) 1971-01-12 1973-02-27 Damon Corp Container unit for liquid samples
US4038055A (en) 1975-10-10 1977-07-26 Block Engineering, Inc. Gas chromatograph for continuous operation with infrared spectrometer
US4168017A (en) 1977-04-05 1979-09-18 Anderwald Cecil E Container means preventing accidental use by children
AU528259B2 (en) 1978-09-14 1983-04-21 Union Carbide Corp Method and apparatus for assaying liquid materials
IT1101295B (it) 1978-11-22 1985-09-28 Erba Strumentazione Camera gas-cromatografica
US4257774A (en) * 1979-07-16 1981-03-24 Meloy Laboratories, Inc. Intercalation inhibition assay for compounds that interact with DNA or RNA
US4420679A (en) 1982-02-26 1983-12-13 Delta Associates, Inc. Gas chromatographic oven using symmetrical flow of preheated - premixed ambient air
US4468423A (en) 1982-11-17 1984-08-28 Arlie Hall Insulating cell element and structures composed thereof
US4468426A (en) * 1982-11-24 1984-08-28 E. I. Du Pont De Nemours And Company Adherable reinforced poly(ethylene terephthalate) composite sheet
US4481405A (en) 1983-04-27 1984-11-06 Malick Franklin S Cooking appliance
US4599169A (en) 1984-02-28 1986-07-08 Varian Associates, Inc. Heating and cooling apparatus for chromatography column
US4701415A (en) 1984-03-02 1987-10-20 Mallinckrodt, Inc. Controlled atmosphere enclosure
JPS60212986A (ja) 1984-04-06 1985-10-25 日本特殊陶業株式会社 直流電圧印加用セラミツクヒ−タ−
JPS60241884A (ja) 1984-05-15 1985-11-30 Tokyo Daigaku 自動サイクリング反応装置およびこれを用いる自動分析装置
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US5333675C1 (en) 1986-02-25 2001-05-01 Perkin Elmer Corp Apparatus and method for performing automated amplification of nucleic acid sequences and assays using heating and cooling steps
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US5038852A (en) 1986-02-25 1991-08-13 Cetus Corporation Apparatus and method for performing automated amplification of nucleic acid sequences and assays using heating and cooling steps
US4965188A (en) 1986-08-22 1990-10-23 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme
JPS6212986A (ja) 1985-07-10 1987-01-21 Hitachi Ltd 磁気バブルメモリデバイスのパツケ−ジ
US4740472A (en) 1985-08-05 1988-04-26 The United States Of America As Represented By The United States Department Of Energy Method and apparatus for automated processing and aliquoting of whole blood samples for analysis in a centrifugal fast analyzer
US4868104A (en) * 1985-09-06 1989-09-19 Syntex (U.S.A.) Inc. Homogeneous assay for specific polynucleotides
US4675300A (en) 1985-09-18 1987-06-23 The Board Of Trustees Of The Leland Stanford Junior University Laser-excitation fluorescence detection electrokinetic separation
CA1273552A (en) * 1985-12-23 1990-09-04 Michael J. Heller Fluorescent stokes shift probes for polynucleotide hybridization assays
ATE88761T1 (de) * 1986-01-10 1993-05-15 Amoco Corp Kompetitiver homogener test.
US4868103A (en) 1986-02-19 1989-09-19 Enzo Biochem, Inc. Analyte detection by means of energy transfer
CA1339653C (en) 1986-02-25 1998-02-03 Larry J. Johnson Appartus and method for performing automated amplification of nucleic acid sequences and assays using heating and cooling steps
US4889818A (en) 1986-08-22 1989-12-26 Cetus Corporation Purified thermostable enzyme
US4708782A (en) 1986-09-15 1987-11-24 Sepragen Corporation Chromatography column-electrophoresis system
US4684465A (en) 1986-10-10 1987-08-04 Combustion Engineering, Inc. Supercritical fluid chromatograph with pneumatically controlled pump
US4902624A (en) 1987-11-23 1990-02-20 Eastman Kodak Company Temperature cycling cuvette
DE3808942A1 (de) 1988-03-17 1989-09-28 Bio Med Gmbh Ges Fuer Biotechn Inkubator, insbes. fuer die polymerase-ketten-methode
GB8807297D0 (en) 1988-03-26 1988-04-27 Dean P D G Intelligent heating block
US4908112A (en) 1988-06-16 1990-03-13 E. I. Du Pont De Nemours & Co. Silicon semiconductor wafer for analyzing micronic biological samples
US4865986A (en) 1988-10-06 1989-09-12 Coy Corporation Temperature control apparatus
US5169511A (en) 1988-11-29 1992-12-08 Isco, Inc. Capillary electrophoresis technique
US5536648A (en) 1988-12-09 1996-07-16 Amrad Corporation Limited Amplified DNA assay using a double stranded DNA binding protein
JPH03505004A (ja) 1989-02-08 1991-10-31 シティ・オブ・ホープ 化学反応処理を連続して行う装置及びその方法
DE69012887D1 (de) 1989-06-20 1994-11-03 Claudio Bonini Prüfröhrchen mit einer linsenförmigen Aussenfläche, insbesondere für automatische klinische Analysen.
US5449621A (en) * 1989-07-31 1995-09-12 Biotope, Inc. Method for measuring specific binding assays
US5346672A (en) 1989-11-17 1994-09-13 Gene Tec Corporation Devices for containing biological specimens for thermal processing
US5075556A (en) * 1989-12-01 1991-12-24 Technicon Instruments Corporation Acridine orange derivatives and their use in the quantitation of reticulocytes in whole blood
US5169521A (en) 1989-12-13 1992-12-08 The United States Of America As Represented By The Department Of Health And Human Services Apparatus for countercurrent chromatography separations
US5173163A (en) 1990-01-11 1992-12-22 Isco, Inc. Capillary electrophoresis technique
US5141621A (en) 1990-01-26 1992-08-25 The Board Of Trustees Of The Leland Stanford Junior University Capillary electrophoresis injection device and method
US5207987A (en) * 1990-05-21 1993-05-04 Pb Diagnostic Systems Inc. Temperature controlled chamber for diagnostic analyzer
EP0459241B1 (en) 1990-05-29 1994-10-26 Waters Investments Limited Process and apparatus for effecting capillary electrophoresis
US5455175A (en) 1990-06-04 1995-10-03 University Of Utah Research Foundation Rapid thermal cycling device
US7081226B1 (en) 1996-06-04 2006-07-25 University Of Utah Research Foundation System and method for fluorescence monitoring
US5187084A (en) 1990-06-22 1993-02-16 The Dow Chemical Company Automatic air temperature cycler and method of use in polymerose chain reaction
US5210015A (en) 1990-08-06 1993-05-11 Hoffman-La Roche Inc. Homogeneous assay system using the nuclease activity of a nucleic acid polymerase
US5269900A (en) 1990-09-13 1993-12-14 University Of North Carolina At Chapel Hill Method and device for high speed separation of complex molecules
DE69128520T2 (de) * 1990-10-31 1998-07-09 Tosoh Corp Verfahren zum Nachweis oder Quantifizierung von Zielnukleinsäuren
US5131998A (en) 1990-11-13 1992-07-21 The University Of North Carolina At Chapel Hill Two-dimensional high-performance liquid chromatography/capillary electrophoresis
US5240577A (en) 1990-11-13 1993-08-31 University Of North Carolina At Chapel Hill Two-dimensional high-performance liquid chromatography/capillary electrophoresis
KR100236506B1 (ko) 1990-11-29 2000-01-15 퍼킨-엘머시터스인스트루먼츠 폴리머라제 연쇄 반응 수행 장치
US5380489A (en) 1992-02-18 1995-01-10 Eastman Kodak Company Element and method for nucleic acid amplification and detection using adhered probes
WO1992017259A1 (en) 1991-03-28 1992-10-15 Perseptive Biosystems, Inc. On-line product identification in a chromatography effluent by subtraction
US5994056A (en) 1991-05-02 1999-11-30 Roche Molecular Systems, Inc. Homogeneous methods for nucleic acid amplification and detection
WO1992020778A1 (en) 1991-05-24 1992-11-26 Kindconi Pty Limited Biochemical reaction control
US5340715A (en) 1991-06-07 1994-08-23 Ciba Corning Diagnostics Corp. Multiple surface evanescent wave sensor with a reference
US5316913A (en) 1991-09-06 1994-05-31 Stanford University Neutrophil LECAM-1 as indicator of neutrophil activation
US5114551A (en) 1991-09-30 1992-05-19 Bio-Rad Laboratories, Inc. Multi-point detection method for electrophoresis and chromatography in capillaries
US5116471A (en) 1991-10-04 1992-05-26 Varian Associates, Inc. System and method for improving sample concentration in capillary electrophoresis
US5565322A (en) * 1991-11-07 1996-10-15 Nanogen, Inc. Hybridization of polynucleotides conjugated with chromophores and fluorophores to generate donor-to donor energy transfer system
US5348853A (en) 1991-12-16 1994-09-20 Biotronics Corporation Method for reducing non-specific priming in DNA amplification
DE4234086A1 (de) * 1992-02-05 1993-08-12 Diagen Inst Molekularbio Verfahren zur bestimmung von in vitro amplifizierten nukleinsaeuresequenzen
EP0566751B1 (en) * 1992-03-23 1996-01-10 F. Hoffmann-La Roche Ag DNA detection method
CA2133643A1 (en) 1992-04-06 1993-10-14 Stanley R. Bouma Method and device for detection of nucleic acid or analyte using total internal reflectance
JPH0634546A (ja) 1992-07-17 1994-02-08 Tosoh Corp 蛍光検出器
WO1994004929A1 (en) 1992-08-24 1994-03-03 Baxter Diagnostics Inc. Sealable vessel for containing and processing analytical samples
US5340728A (en) * 1992-12-09 1994-08-23 E. I. Du Pont De Nemours And Company Method for amplification of targeted segments of nucleic acid using nested polymerase chain reaction
US5593840A (en) 1993-01-27 1997-01-14 Oncor, Inc. Amplification of nucleic acid sequences
US5364790A (en) 1993-02-16 1994-11-15 The Perkin-Elmer Corporation In situ PCR amplification system
US5798215A (en) 1993-02-18 1998-08-25 Biocircuits Corporation Device for use in analyte detection assays
US5436134A (en) 1993-04-13 1995-07-25 Molecular Probes, Inc. Cyclic-substituted unsymmetrical cyanine dyes
US6027884A (en) * 1993-06-17 2000-02-22 The Research Foundation Of The State University Of New York Thermodynamics, design, and use of nucleic acid sequences
JP3598123B2 (ja) 1993-07-15 2004-12-08 浜松ホトニクス株式会社 核酸の変性検出装置
ATE208658T1 (de) 1993-07-28 2001-11-15 Pe Corp Ny Vorrichtung und verfahren zur nukleinsäurevervielfältigung
CA2129787A1 (en) * 1993-08-27 1995-02-28 Russell G. Higuchi Monitoring multiple amplification reactions simultaneously and analyzing same
US5670315A (en) * 1993-09-13 1997-09-23 Canon Kabushiki Kaisha Nucleic acid determination employing pyryilium dye
US5415839A (en) 1993-10-21 1995-05-16 Abbott Laboratories Apparatus and method for amplifying and detecting target nucleic acids
US5863726A (en) 1993-11-12 1999-01-26 Geron Corporation Telomerase activity assays
US5804380A (en) 1993-11-12 1998-09-08 Geron Corporation Telomerase activity assays
ES2386007T3 (es) 1993-11-12 2012-08-07 Phri Properties, Inc. Sonda de hibridación para la detección de ácido nucléico, tallos universales, métodos y kits
US5925517A (en) * 1993-11-12 1999-07-20 The Public Health Research Institute Of The City Of New York, Inc. Detectably labeled dual conformation oligonucleotide probes, assays and kits
GB9401535D0 (en) 1994-01-27 1994-03-23 Steer Rupert P Timing devices
AU699986B2 (en) 1994-02-01 1998-12-17 Igene Inc. Molecular analyzer and method of use
US5654419A (en) 1994-02-01 1997-08-05 The Regents Of The University Of California Fluorescent labels and their use in separations
JP3265113B2 (ja) * 1994-03-04 2002-03-11 三菱製紙株式会社 インクジェット記録シート
CA2143365A1 (en) 1994-03-14 1995-09-15 Hugh V. Cottingham Nucleic acid amplification method and apparatus
CA2148140C (en) 1994-04-29 2010-08-10 John W. Sutherland Homogeneous method for assay of double-stranded nucleic acids using fluorescent dyes and kit useful therein
JP2909216B2 (ja) * 1994-04-29 1999-06-23 パーキン‐エルマー コーポレイション 核酸増幅生成物のリアルタイム検出装置
JPH10500572A (ja) * 1994-05-23 1998-01-20 バイオトロニクス コーポレーション 標的核酸を検出する方法
US5766889A (en) 1994-06-08 1998-06-16 The Perkin-Elmer Corporation Method for determining the characteristics of the concentration growth of target nucleic acid molecules in polymerase chain reaction sample
US5622821A (en) * 1994-06-29 1997-04-22 The Regents Of The University Of California Luminescent lanthanide chelates and methods of use
DE69527125T2 (de) * 1994-11-09 2003-02-27 Hitachi, Ltd. Verfahren und Vorrichtung zur DNS Analyse
US6008373A (en) * 1995-06-07 1999-12-28 Carnegie Mellon University Fluorescent labeling complexes with large stokes shift formed by coupling together cyanine and other fluorochromes capable of resonance energy transfer
EP0832280A2 (en) * 1995-06-07 1998-04-01 Abbott Laboratories Probe masking method of reducing background in an amplification reaction
WO1996041864A1 (en) 1995-06-13 1996-12-27 The Regents Of The University Of California Diode laser heated micro-reaction chamber with sample detection means
US5945526A (en) * 1996-05-03 1999-08-31 Perkin-Elmer Corporation Energy transfer dyes with enhanced fluorescence
US5863727A (en) * 1996-05-03 1999-01-26 The Perkin-Elmer Corporation Energy transfer dyes with enhanced fluorescence
US5800996A (en) * 1996-05-03 1998-09-01 The Perkin Elmer Corporation Energy transfer dyes with enchanced fluorescence
ATE318327T1 (de) * 1996-06-04 2006-03-15 Univ Utah Res Found Fluoreszenz-donor-akzeptor paar
US5846727A (en) 1996-06-06 1998-12-08 Board Of Supervisors Of Louisiana State University And Agricultural & Mechanical College Microsystem for rapid DNA sequencing
EP0822261B1 (en) * 1996-07-29 2002-07-03 Academia Sinica Methods for rapid antimicrobial susceptibility testing
US6482590B1 (en) 1996-12-20 2002-11-19 Aventis Behring Gmbh Method for polynucleotide amplification
US6143496A (en) * 1997-04-17 2000-11-07 Cytonix Corporation Method of sampling, amplifying and quantifying segment of nucleic acid, polymerase chain reaction assembly having nanoliter-sized sample chambers, and method of filling assembly
US6140054A (en) * 1998-09-30 2000-10-31 University Of Utah Research Foundation Multiplex genotyping using fluorescent hybridization probes
US6153411A (en) * 1998-10-30 2000-11-28 American Water Works Company, Inc. Methods and kits for detection of Cryptosporidium parvum using immunomagnetic separation and amplification

Also Published As

Publication number Publication date
EP0912766B2 (en) 2011-12-14
JP4401416B2 (ja) 2010-01-20
ATE428801T1 (de) 2009-05-15
EP1033411A3 (en) 2000-10-11
JP2000509608A (ja) 2000-08-02
US20060029965A1 (en) 2006-02-09
DK0912766T4 (da) 2012-04-02
US20090258414A1 (en) 2009-10-15
JP2008173127A (ja) 2008-07-31
EP1179600A1 (en) 2002-02-13
US7160998B2 (en) 2007-01-09
DE69739357D1 (de) 2009-05-28
US20090311673A1 (en) 2009-12-17
DE69735313T2 (de) 2006-11-02
CA2257109A1 (en) 1997-12-11
CA2257109C (en) 2009-10-06
NZ333136A (en) 2000-03-27
PT912766E (pt) 2009-07-16
WO1997046714A1 (en) 1997-12-11
DK1179600T3 (da) 2005-09-05
JP4540754B2 (ja) 2010-09-08
US8343754B2 (en) 2013-01-01
CA2658290C (en) 2012-04-10
EP1179600B1 (en) 2005-05-11
NZ502323A (en) 2001-09-28
US7670832B2 (en) 2010-03-02
US20040002098A1 (en) 2004-01-01
DE69735313D1 (de) 2006-04-27
ES2243393T3 (es) 2005-12-01
AU726501B2 (en) 2000-11-09
EP1033411B1 (en) 2006-02-22
EP0912766A1 (en) 1999-05-06
EP0912766B1 (en) 2009-04-15
DE69733282D1 (de) 2005-06-16
DE69733282T2 (de) 2006-01-19
AU3481297A (en) 1998-01-05
ES2326050T5 (es) 2012-04-26
US6245514B1 (en) 2001-06-12
US6569627B2 (en) 2003-05-27
DK0912766T3 (da) 2009-08-10
ATE318327T1 (de) 2006-03-15
EP1033411A2 (en) 2000-09-06
US6232079B1 (en) 2001-05-15
JP2007185188A (ja) 2007-07-26
ATE295427T1 (de) 2005-05-15
JP4118932B2 (ja) 2008-07-16
US20120258524A1 (en) 2012-10-11
CA2658290A1 (en) 1997-12-11
JP2010046063A (ja) 2010-03-04
US6174670B1 (en) 2001-01-16
CA2591550C (en) 2009-04-28
CA2591550A1 (en) 1997-12-11
US20020058258A1 (en) 2002-05-16
ES2326050T3 (es) 2009-09-29

Similar Documents

Publication Publication Date Title
PT1179600E (pt) Monitorizacao da hibridacao durante a rcp
EA200300284A1 (ru) Способ амплификации нуклеиновых кислот
DE69734283D1 (de) Met/fret markierte amplifikationsoligonukleotide und ihre vewendung in verfahren
DE69939902D1 (de) Wellenlängen verschiebende proben und primer
DK0699768T3 (da) Fremgangsmåder til quenching i opløsning af fluorescens-mærkede oligonnukleotidprober
EA200400329A1 (ru) Способы амплификации нуклеиновых кислот
CA2255531A1 (en) Labeled primer for use in and detection of target nucleic acids
PT702090E (pt) Co-amplificacao de acidos nucleicos alvo utilizando agentes de exclusao de volume na composicao da reaccao, kit de teste e respectivo dispositivo de teste
DK0776981T3 (da) Oligonucleotidprimere til amplifikation af HCV-nukleinsyre
SE8703675L (sv) Luminometrisk analys paa cellulaert atp
ES2094178T3 (es) Secuencias nucleotidicas utiles como sondas especificas del tipo cebadores pct y sondas para la amplificacion y deteccion del virus del papiloma humano, y kits y procedimientos utilizados con este fin.
ATE203775T1 (de) Methode zur detektion einer gezielten nukleinsäure
GB9805935D0 (en) Organic compounds
WO2001036683A3 (en) Method for rapid and accurate identification of microorganisms
JP6961700B2 (ja) 生体サンプル中の遺伝物質を検出する方法及びその実施のための装置
DE602004028993D1 (de) Neues Format zur Detektion von Heissstart- und Echtzeit-Polymerasekettenreaktion
EP0919633A3 (en) Detection of Neisseria Gonorrhoeae by amplification and detection of its nucleic acid
AU1514397A (en) Amplifying and detecting target nucleic acids using a post amplification incubation step
WO2022040443A3 (en) A rapid diagnostic test for lamp
DE60038029D1 (de) Methoden und reagenzien zur in situ amplifizierung
DE69910026D1 (de) Mehrfach fluoreszierende und energie übertragende hairpin-oligonukleotide
ATE455867T1 (de) Echtzeit-pcr unter zusatz von pyrophosphatase
DE60208299D1 (de) Detektion von amplifizierten Genprodukten zur Gendetektion und Reagenzkit
ES2188357B1 (es) Metodo para la amplificacion mediante transcripcion en reverso acoplada a una reaccion de nested o semi-nested pcr en un solo paso, de un arn diana, y sus aplicaciones en la identificacion de enterovirus y en la discriminacion entre poliovirus y enterovirus no-polio.
ES2121698B1 (es) Procedimiento para la obtencionn de fragmentos amplificados de acido nucleico de particulas virales y subvirales que contienen rna.