BR0210610A - Métodos e oligonucleotìdeos para detecção de salmonella sp., e.coli 0157:h7 e listeria monocitogenes - Google Patents

Métodos e oligonucleotìdeos para detecção de salmonella sp., e.coli 0157:h7 e listeria monocitogenes

Info

Publication number
BR0210610A
BR0210610A BR0210610-8A BR0210610A BR0210610A BR 0210610 A BR0210610 A BR 0210610A BR 0210610 A BR0210610 A BR 0210610A BR 0210610 A BR0210610 A BR 0210610A
Authority
BR
Brazil
Prior art keywords
salmonella
coli
methods
oligonucleotides
detection
Prior art date
Application number
BR0210610-8A
Other languages
English (en)
Inventor
Jay L E Ellingson
Dirk Neuville Vevea
Original Assignee
Marshfield Clinic
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Marshfield Clinic filed Critical Marshfield Clinic
Publication of BR0210610A publication Critical patent/BR0210610A/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

"MéTODOS E OLIGONUCLEOTìDEOS PARA DETECçãO DE SALMONELLA SP., E.COLI 0157:H7 E LISTERIA MONOCITOGENES". Revela-se um método para detectar uma espécie Salmonella, E. coli 0157:H7 ou Listeria monocitogenes. O método envolve a ampliação de uma seq³ência de nucleotídeo genómica de uma espécie correspondente e detectar o produto de ampliação. Vários primers e sondas que podem ser usados no métodos também são apresentados. Em uma modalidade, a etapa de ampliação do método é realizada por PCR em tempo real e o produto de ampliação é detectado por transferência de energia de ressonância fluorescente usando um par de polinucleotídeos rotulados.
BR0210610-8A 2001-06-22 2002-06-21 Métodos e oligonucleotìdeos para detecção de salmonella sp., e.coli 0157:h7 e listeria monocitogenes BR0210610A (pt)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US30019901P 2001-06-22 2001-06-22
US37358802P 2002-04-18 2002-04-18
US37358902P 2002-04-18 2002-04-18
PCT/US2002/021181 WO2003000935A1 (en) 2001-06-22 2002-06-21 Methods and oligonucleotides for the detection of salmonella sp.,e.coli o157:h7,and listeria monocytogenes

Publications (1)

Publication Number Publication Date
BR0210610A true BR0210610A (pt) 2005-04-19

Family

ID=27404719

Family Applications (1)

Application Number Title Priority Date Filing Date
BR0210610-8A BR0210610A (pt) 2001-06-22 2002-06-21 Métodos e oligonucleotìdeos para detecção de salmonella sp., e.coli 0157:h7 e listeria monocitogenes

Country Status (7)

Country Link
US (1) US7241566B2 (pt)
EP (1) EP1409738A4 (pt)
BR (1) BR0210610A (pt)
CA (1) CA2451498A1 (pt)
MX (1) MXPA03012075A (pt)
NZ (1) NZ530343A (pt)
WO (1) WO2003000935A1 (pt)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2003050269A2 (en) * 2001-12-11 2003-06-19 Arizona Board Of Regents HIGH RESOLUTION TYPING SYSTEM FOR PATHOGENIC $i(E. COLI)
ES2223215B1 (es) * 2002-03-11 2006-04-16 Universidad De Sevilla Metodo para la deteccion e identificacion rapido de bacillus cereus mediante la reaccion en cadena de la polimerasa en tiempo real.
US20060177824A1 (en) * 2003-04-29 2006-08-10 Procop Gary W Salmonella detection identification
US7460223B2 (en) * 2003-09-19 2008-12-02 Applied Biosystems Inc. Inverted orientation for a microplate
CA2566949C (en) 2004-05-20 2015-03-24 Warnex Research Inc. Polynucleotides for the detection of escherichia coli o157:h7 and escherichia coli o157:nm verotoxin producers
US20060246463A1 (en) * 2005-04-20 2006-11-02 Vevea Dirk N Methods and oligonucleotides for the detection of Salmonella SP., E coli 0157:H7, and Listeria monocytogenes
US20060240442A1 (en) * 2005-04-20 2006-10-26 Vevea Dirk N Methods and oligonucleotides for the detection of Salmonella SP., E coli 0157:H7, and Listeria monocytogenes
DE102005037796A1 (de) * 2005-08-07 2007-02-08 Tgc Biomics Gmbh Testsystem zum Nachweis von Salmonellen
US7833740B2 (en) 2005-08-07 2010-11-16 Tgc Biomics Gmbh Test system for detecting salmonella
US20080148630A1 (en) * 2006-12-20 2008-06-26 Institute For Technology Development Methods and systems for growing plants using diffuse scattering illumination
EP2646569A1 (en) 2010-11-30 2013-10-09 Diagon Kft. Procedure for nucleic acid-based molecular diagnostic determination of bacterial germ counts and kit for this purpose

Family Cites Families (81)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4689295A (en) * 1983-01-20 1987-08-25 Integrated Genetics, Inc. Test for Salmonella
FR2567541B1 (fr) * 1984-07-13 1987-02-06 Pasteur Institut Sonde d'adn et procede pour la detection de " shigelles " et des souches entero-invasives de escherichia coli
US5541308A (en) * 1986-11-24 1996-07-30 Gen-Probe Incorporated Nucleic acid probes for detection and/or quantitation of non-viral organisms
US6150517A (en) * 1986-11-24 2000-11-21 Gen-Probe Methods for making oligonucleotide probes for the detection and/or quantitation of non-viral organisms
ATE133454T1 (de) * 1987-12-01 1996-02-15 Amoco Corp Nachweis von salmonella
WO1989006699A1 (en) 1988-01-13 1989-07-27 Institut Pasteur Dna probe specific for hemolytic listeria
CA1340807C (en) * 1988-02-24 1999-11-02 Lawrence T. Malek Nucleic acid amplification process
US5130238A (en) * 1988-06-24 1992-07-14 Cangene Corporation Enhanced nucleic acid amplification process
JPH0638759B2 (ja) * 1988-07-29 1994-05-25 株式会社島津製作所 サルモネラ菌検査に用いるｄｎａプローブ及びそれを用いたサルモネラ菌の検出法
US5523205A (en) * 1988-08-02 1996-06-04 Institut Pasteur DNA probes specific for hemolytic listeria
US5041372A (en) * 1988-11-02 1991-08-20 The United States Of America As Represented By The Department Of Health And Human Services Probe to identify enteroinvasive E. coli and Shigella species
CA2025236A1 (en) 1989-02-06 1990-08-07 Walter King Probes and methods for the detection of listeria
CA2009708A1 (en) 1989-02-13 1990-08-13 Jane D. Madonna Nucleic acid probe for the detection of salmonella human pathogens
IE81145B1 (en) * 1989-04-20 2000-05-03 Thomas Gerard Barry Generation of specific probes for target nucleotide sequences
US5043272A (en) * 1989-04-27 1991-08-27 Life Technologies, Incorporated Amplification of nucleic acid sequences using oligonucleotides of random sequence as primers
DE69022167T2 (de) 1989-05-23 1996-02-15 Amoco Corp Nukleinsäuresonden zum nachweis von staphylococcus aureus.
JPH0789958B2 (ja) 1989-07-18 1995-10-04 株式会社島津製作所 サルモネラ菌検出のためのオリゴヌクレオチドおよびそれを用いた検出法
EP0409159B1 (en) * 1989-07-18 1998-11-25 Shimadzu Corporation Method for testing causative microorganism of food poisoning and reagents therefor
CA2033718A1 (en) * 1990-01-19 1991-07-20 Ronald M. Atlas Process for detection of water-borne microbial pathogens and indicators of human fecal contamination in water samples and kits therefor
JPH04228097A (ja) * 1990-07-02 1992-08-18 Toyo Ink Mfg Co Ltd ミルク試料からの細胞分離および濃縮方法とそのキット
FR2664614B1 (fr) * 1990-07-11 1992-10-16 Pasteur Institut Sequences nucleiques issues du genome de salmonella typhi, et leurs utilisations notamment pour le diagnostic in vitro de la presence de bacteries du genre salmonella dans les produits alimentaires.
US5491068A (en) 1991-02-14 1996-02-13 Vicam, L.P. Assay method for detecting the presence of bacteria
US6028187A (en) * 1991-08-01 2000-02-22 Gen-Probe Incorporated Nucleic acid probes to Listeria monocytogenes
IL103935A0 (en) * 1991-12-04 1993-05-13 Du Pont Method for the identification of microorganisms by the utilization of directed and arbitrary dna amplification
US5468852A (en) * 1992-02-18 1995-11-21 Shimadzu Corporation Oligonucleotides for detecting bacteria
EP0576842B1 (de) * 1992-06-11 2002-10-16 MERCK PATENT GmbH Verfahren und Mittel zum Nachweis von Listerien
US6372424B1 (en) * 1995-08-30 2002-04-16 Third Wave Technologies, Inc Rapid detection and identification of pathogens
US5340728A (en) 1992-12-09 1994-08-23 E. I. Du Pont De Nemours And Company Method for amplification of targeted segments of nucleic acid using nested polymerase chain reaction
GB9312508D0 (en) * 1993-06-17 1993-08-04 Bioteknologisk Inst Compounds
DE4337295A1 (de) * 1993-11-02 1995-05-04 Merck Patent Gmbh Oligonukleotide für den Nachweis von Enterobacteriaceae
EP0669399B1 (en) * 1994-02-28 2003-10-01 Shimadzu Corporation Oligonucleotides and method for detecting bacteria
US5610012A (en) * 1994-04-08 1997-03-11 Wisconsin Alumni Research Foundation DNA probes specific for virulent listeria monocytogenes
US5705332A (en) * 1994-04-25 1998-01-06 University Of Hawaii Detection and identification of Salmonella and Shigella
US5660981A (en) * 1994-06-06 1997-08-26 E. I. Du Pont De Nemours And Company Selection of diagnostic genetic markers in microorganisms and use of a specific marker for detection of salmonella
US5475098A (en) * 1994-06-14 1995-12-12 The United States Of America As Represented By The Department Of Health And Human Services Distinctive DNA sequence of E. coli 0157:H7 and its use for the rapid, sensitive and specific detection of 0157:H7 and other enterohemorrhagic E. coli
US20020055101A1 (en) * 1995-09-11 2002-05-09 Michel G. Bergeron Specific and universal probes and amplification primers to rapidly detect and identify common bacterial pathogens and antibiotic resistance genes from clinical specimens for routine diagnosis in microbiology laboratories
US6001564A (en) 1994-09-12 1999-12-14 Infectio Diagnostic, Inc. Species specific and universal DNA probes and amplification primers to rapidly detect and identify common bacterial pathogens and associated antibiotic resistance genes from clinical specimens for routine diagnosis in microbiology laboratories
US5786454A (en) * 1994-09-16 1998-07-28 Washington University School Of Medicine Modified SH2 domains
US5652102A (en) * 1994-12-05 1997-07-29 The United States Of America As Represented By The Secretary Of Agriculture Assay for enterohemorrhagic Escherichia coli 0157:H7 by the polymerase chain reaction
CA2139070C (en) * 1994-12-23 2010-03-30 Burton W. Blais Method for enhancing detection ability of nucleic acid assays employing polymerase chain reaction
FR2729392B1 (fr) * 1995-01-16 1997-03-28 Pasteur Institut Oligonucleotides pour la detection de salmonella
US5654417A (en) * 1995-04-14 1997-08-05 Children's Hospital And Medical Center Nucleic acid probes for detecting E. coli O157:H7
US5654144A (en) * 1995-04-24 1997-08-05 The United States Of America As Represented By The Secretary Of The Army Detection of Yersinia using the polymerase chain reaction
US5853987A (en) * 1995-04-24 1998-12-29 The Texas A & M University System Decorin binding protein compositions and methods of use
US5708160A (en) * 1995-04-26 1998-01-13 The National Research Council HSP-60 genomic locus and primers for species identification
JP2792462B2 (ja) * 1995-04-28 1998-09-03 株式会社島津製作所 サルモネラ属菌検出のためのオリゴヌクレオチドおよびそれを用いた検出法
US5843650A (en) * 1995-05-01 1998-12-01 Segev; David Nucleic acid detection and amplification by chemical linkage of oligonucleotides
US5994066A (en) * 1995-09-11 1999-11-30 Infectio Diagnostic, Inc. Species-specific and universal DNA probes and amplification primers to rapidly detect and identify common bacterial pathogens and associated antibiotic resistance genes from clinical specimens for routine diagnosis in microbiology laboratories
US5747256A (en) * 1995-12-19 1998-05-05 Beckman Instruments, Inc. Homogeneous DNA probe titration assay
US5612473A (en) * 1996-01-16 1997-03-18 Gull Laboratories Methods, kits and solutions for preparing sample material for nucleic acid amplification
US5747257A (en) 1996-02-29 1998-05-05 E. I. Du Pont De Nemours And Company Genetic markers and methods for the detection of escherichia coli serotype-0157:H7
US5723294A (en) * 1996-03-05 1998-03-03 Gull Laboratories Methods for detection and discrimination of multiple analytes using fluorescent technology
CA2255774C (en) * 1996-05-29 2008-03-18 Cornell Research Foundation, Inc. Detection of nucleic acid sequence differences using coupled ligase detection and polymerase chain reactions
CA2257109C (en) * 1996-06-04 2009-10-06 University Of Utah Research Foundation Monitoring hybridization during pcr
US6312930B1 (en) * 1996-09-16 2001-11-06 E. I. Du Pont De Nemours And Company Method for detecting bacteria using PCR
US5922536A (en) * 1996-10-18 1999-07-13 Nivens; David E. Method for nucleic acid isolation using supercritical fluids
US5958686A (en) * 1996-10-28 1999-09-28 The United States Of America As Represented By The Secretary Of The Army Simple PCR technique for detecting and differentiating bacterial pathogens
WO1998020148A1 (en) 1996-11-04 1998-05-14 The Regents Of The University Of California Method for detection of pathogens in food
US5922538A (en) * 1996-11-08 1999-07-13 E.I. Du Pont De Nemours And Company Genetic markers and methods for the detection of Listeria monocytogenes and Listeria spp
GB9624165D0 (en) * 1996-11-19 1997-01-08 Amdex A S Use of nucleic acids bound to carrier macromolecules
AU6534898A (en) 1997-02-14 1998-09-08 E.I. Du Pont De Nemours And Company Detection of double-stranded dna in a homogeneous solution
US5925522A (en) * 1997-05-09 1999-07-20 Battelle Memorial Institute Salmonella nucleotide sequences, methods of detection of salmonella nucleotide sequences, and method of detection of salmonella
US6235480B1 (en) * 1998-03-13 2001-05-22 Promega Corporation Detection of nucleic acid hybrids
AU6750198A (en) 1998-04-03 1999-10-25 Seed Capital Investments-2 (Sci-2) B.V. A method of interstrain differentiation of bacteria
JPH11332599A (ja) * 1998-05-29 1999-12-07 Shimadzu Corp 腸管出血性大腸菌検出のためのオリゴヌクレオチドおよびそれを用いた検出法
US6503747B2 (en) * 1998-07-14 2003-01-07 University Of Hawaii Serotype-specific probes for Listeria monocytogenes
US6268143B1 (en) * 1998-08-05 2001-07-31 Kansas State University Research Foundation Automated high throughput E. coli o157:H7 PCR detection system and uses thereof
DE19840044A1 (de) 1998-09-02 2000-03-23 Biotecon Ges Fuer Biotechnologische Entwicklung & Consulting Mbh Oligonukleotide, Verfahren und Kit zur Detektion von Listeria monocytogenes durch Nukleinsäureamplifikation und/oder -hybridisierung
JP2000093191A (ja) 1998-09-18 2000-04-04 Nippon Zeon Co Ltd 光学活性化合物の製造方法
WO2000017399A2 (en) * 1998-09-18 2000-03-30 The Board Of Regents Of The University Of Nebraska High resolution genome scanning
US6291168B1 (en) * 1998-10-27 2001-09-18 Auburn University Nucleic acid sequences diagnostic for pathogenic E.coli O157:H7, methods of identification and kit therefore
US6365723B1 (en) * 1998-12-04 2002-04-02 Wisconsin Alumni Research Foundation Sequences of E. coli O157
US6060252A (en) * 1999-04-12 2000-05-09 Becton Dickinson And Company Amplification and detection of shigella spp. and enteroinvasive strains of Escherichia coli
US6165721A (en) * 1999-04-12 2000-12-26 Becton Dickinson And Company Amplification and detection of Salmonella spp
US20020086289A1 (en) * 1999-06-15 2002-07-04 Don Straus Genomic profiling: a rapid method for testing a complex biological sample for the presence of many types of organisms
WO2000077247A1 (en) 1999-06-15 2000-12-21 University Of Guelph A marker specific for escherichia coli serotypes o157:h7; o157:nm and o55:h7
US6723505B1 (en) 1999-08-13 2004-04-20 Nye Colifast As Method for identification of the indicators of contamination in liquid samples
US6251607B1 (en) * 1999-12-09 2001-06-26 National Science Council Of Republic Of China PCR primers for the rapid and specific detection of Salmonella typhimurium
US6322985B1 (en) * 1999-12-27 2001-11-27 Technion Research And Development Foundation Ltd. Abundant, well distributed and hyperpolymorphic simple sequence repeats in prokaryote genomes and use of same for prokaryote classification and typing
EP1349958B1 (en) * 2000-09-26 2009-06-03 Boston Probes, Inc. Probes, probe sets, methods and kits pertaining to the detection, identification and/or enumeration of bacteria
US9519254B2 (en) * 2014-12-17 2016-12-13 Lexmark International, Inc. Systems for configuring settings of an electronic device for customization thereof

Also Published As

Publication number Publication date
US7241566B2 (en) 2007-07-10
WO2003000935A1 (en) 2003-01-03
EP1409738A4 (en) 2005-03-30
MXPA03012075A (es) 2005-07-01
CA2451498A1 (en) 2003-01-03
US20030022214A1 (en) 2003-01-30
EP1409738A1 (en) 2004-04-21
NZ530343A (en) 2007-01-26

Similar Documents

Publication Publication Date Title
Isacsson et al. Rapid and specific detection of PCR products using light-up probes
JP4632788B2 (ja) 核酸のヘリカーゼ依存性増幅
HUP0101385A2 (hu) Eljárás nukleinsavak detektálására PCR-módszerrel
DE60121342D1 (de) Einfach markierte oligonukleotidsonden
CN107760762A (zh) 一种检测dna腺嘌呤甲基转移酶的荧光化学传感器及其检测方法
PT1633888E (pt) Meios e método para a detecção de sequências de nucleótidos alvo utilizando ensaios de ligação com pares de sondas de oligonucleótido melhoradas
ATE394508T1 (de) Late-pcr
WO2001096612A3 (en) Method of identifying and quantifying specific fungi and bacteria
BR0210610A (pt) Métodos e oligonucleotìdeos para detecção de salmonella sp., e.coli 0157:h7 e listeria monocitogenes
DK1159453T3 (da) Fremgangsmåde til direkte nukleinsyresekventering
KR970028548A (ko) 핵산의 형광 편광도 검출방법
WO2004059289A3 (en) Target-dependent transcription using deletion mutants of n4 rna polymerase
DE60132924D1 (de) Zusammensetzungen und methoden zur detektion von humanem immundefizienz virus 2 (hiv-2)
WO2004044164A3 (en) Method for identifying risk of melanoma and treatments thereof
DE60333480D1 (de) Verfahren zum nachweis von pathogenen organismen
ATE330031T1 (de) Reversibel inhibierende sonden
Kassabov et al. Site-directed histone–DNA contact mapping for analysis of nucleosome dynamics
ATE305051T1 (de) Genetische marker und methoden zum detektion von escherichia coli serotype-0157:h7
JP2007125011A (ja) ポリメラーゼ連鎖反応の助けによりｄｎａ又はｒｎａの特定のフラグメントを検出する方法
JP2018183065A (ja) Ｒｎａ検出方法
WO2002095066A3 (de) Nachweis und differenzierung von mikroorganismen der spezies yersinia pestis/yersinia pseudotuberculosis
JPH11512602A (ja) Ｐｃｒのための対照
US20120058515A1 (en) Method of regulating oligonucleotide functionality
ATE335849T1 (de) Verfahren und nukleinsäuren zum nachweis von brauereirelevanten mikroorganismen
Kol et al. Polymorphism of ISSR-PCR markers in Tuvinian population of reindeer Rangifer tarandus L.

Legal Events

Date Code Title Description
B08F Application dismissed because of non-payment of annual fees [chapter 8.6 patent gazette]

Free format text: REFERENTE 6A., 7A., E 8A. ANUIDADES.

B08K Patent lapsed as no evidence of payment of the annual fee has been furnished to inpi [chapter 8.11 patent gazette]

Free format text: REFERENTE AO DESPACHO 8.6 PUBLICADO NA RPI 2060 DE 29/06/2010.